Digital Commons Network^™

Between Two Fern Genomes, E. B. Sessa, J. A. Banks, M. S. Barker, J. P. Der, A. M. Duffy, S. W. Graham, M. Hasebe, J. Langdale, F. W. Li, D. B. Marchant, K. M. Pryer, C. J. Rothfels, S. J. Roux, M. L. Salmi, E. M. Sigel, D. E. Soltis, P. S. Soltis, D. W. Stevenson, Paul G. Wolf

Biology Faculty Publications

Ferns are the only major lineage of vascular plants not represented by a sequenced nuclear genome. This lack of genome sequence information significantly impedes our ability to understand and reconstruct genome evolution not only in ferns, but across all land plants. Azolla and Ceratopteris are ideal and complementary candidates to be the first ferns to have their nuclear genomes sequenced. They differ dramatically in genome size, life history, and habit, and thus represent the immense diversity of extant ferns. Together, this pair of genomes will facilitate myriad large-scale comparative analyses across ferns and all land plants. Here we review the …

Production Of Destruxins From Metarhizium Spp. Fungi In Artificial Medium And In Endophytically Colonized Cowpea Plants, P. S. Golo, D. R. Gardner, Michelle M. Grilley, Jon Y. Takemoto, S. B. Krasnoff, M. S. Pires, E. K. K. Férnandes, V. R. Bittencourt, Donald W. Roberts

Biology Faculty Publications

Destruxins (DTXs) are cyclic depsipeptides produced by many Metarhizium isolates that have long been assumed to contribute to virulence of these entomopathogenic fungi. We evaluated the virulence of 20 Metarhizium isolates against insect larvae and measured the concentration of DTXs A, B, and E produced by these same isolates in submerged (shaken) cultures. Eight of the isolates (ARSEF 324, 724, 760, 1448, 1882, 1883, 3479, and 3918) did not produce DTXs A, B, or E during the five days of submerged culture. DTXs were first detected in culture medium at 2–3 days in submerged culture. Galleria mellonella and Tenebrio molitor …

Size Dependent Translocation And Fetal Accumulation Of Gold Nanoparticles From Maternal Blood In The Rat, Manuela Semmler-Behnke, Jens Lipka, Alexander Wenk, Martin Schaeffer, Furong Tian, Gunter Schmid, Gunter Oberdorster, Wolfgang Kreyling

Articles

There is evidence that nanoparticles (NP) cross epithelial and endothelial body barriers. We hypothesized that gold (Au) NP, once in the blood circulation of pregnant rats, will cross the placental barrier during pregnancy size-dependently and accumulate in the fetal organism by 1. transcellular transport across the hemochorial placenta, 2. transcellular transport across amniotic membranes 3. transport through ~20 nm wide transtrophoblastic channels in a size dependent manner. The three AuNP sizes used to test this hypothesis are either well below, or of similar size or well above the diameters of the transtrophoblastic channels.

Commitment Types And Influence Of Daily Deals Among Boot Camp Participants, Linda Lund

Master's Theses

The sedentary lifestyle that many Americans exhibit reinforces the need for continued research into the factors associated with the adoption and maintenance of regular physical activity. Internet-based daily deal websites, such as Groupon® and LivingSocial®, feature deals and discounts on many products and services, including fitness programs. The purpose of this study was to determine the relationship between "daily deals" usage and the types of commitment exhibited by fitness program participants. The targeted program studied was a fitness boot camp. Boot camp participants (N=150), recruited from an indoor boot camp company, completed a questionnaire that included basic demographic information, membership …

Vortioxetine Dose-Dependently Reverses 5-Ht Depletion-Induced Deficits In Spatial Working And Object Recognition Memorya Potential Role For 5-Ht1a Receptor Agonism And 5-Ht3 Receptor Antagonism, Kristian Gaarn Du Jardin, Jesper Bornø Jensen, Connie Sanchez, Alan Pehrson

Department of Psychology Faculty Scholarship and Creative Works

We previously reported that the investigational multimodal antidepressant, vortioxetine, reversed 5-HT depletion-induced memory deficits while escitalopram and duloxetine did not. The present report studied the effects of vortioxetine and the potential impact of its 5-HT1A receptor agonist and 5-HT3 receptor antagonist properties on 5-HT depletion-induced memory deficits. Recognition and spatial working memory were assessed in the object recognition (OR) and Y-maze spontaneous alternation (SA) tests, respectively. 5-HT depletion was induced in female Long-Evans rats using 4-cholro-DL-phenylalanine methyl ester HCl (PCPA) and receptor occupancies were determined by ex vivo autoradiography. Rats were acutely dosed with vortioxetine, ondansetron (5-HT3 receptor antagonist) or …

Project Shine: A Family-Based Intervention For Improving Physical Activity, Sedentary Behavior, And Diet In African American Adolescents, Sara M. St. George

Theses and Dissertations

This study examined the effects of a family-based intervention for improving moderate-to-vigorous physical activity (MVPA), sedentary behavior (SB), and fruit and vegetable (F&V) intake in African American adolescents. The intervention (Project SHINE: Supporting Health Interactively through Nutrition and Exercise) integrated Social Cognitive (SCT), Self Determination (SDT), and Family Systems Theories (FST) to improve healthy physical activity and dietary behaviors. Behavioral strategies from SCT (i.e., self-monitoring, goal-setting, self-regulatory skill-building), elements involved in facilitating intrinsic motivation for health behavior change from SDT (i.e., autonomy, competence, belongingness), and positive parenting practices from FST for integrating parent and peer systems (e.g., parental monitoring, parent-adolescent …

Does Growth In Childhood Adhd And Depression Symptoms Predict Binge-Eating During Adolescence, Kathryn Van Eck

Theses and Dissertations

Binge-eating disorder (BED) refers to experiencing a loss of control while eating high quantities of food in a short period of time. A serious public health concern, BED is highly comorbid with other psychological disorders and increases risk for obesity and other health concerns, such as metabolic disorder and diabetes. Little is known about what mental health symptoms contribute to the development of BED for children and adolescents. Research with adults indicates that two strong predictors of binge-eating behavior include impulsivity and depression, and these symptom areas may contribute to BED for youth, as well. In the current study, I …

The Regulation Of Skeletal Muscle Mass And Mitochondrial Biogenesis By Gp130/Stat3 Signaling During Cancer Cachexia, Melissa Puppa

Theses and Dissertations

Cachexia affects nearly 70% of all cancer patients depending on the cancer, and decreases cancer survival. Cachexia is associated with muscle mass loss that is accompanied by a loss in muscle oxidative capacity, a decrease in protein synthesis and an increase in protein degradation. While progress has been made in understanding some of the mechanisms underlying the cachectic condition, there are currently no approved pharmaceutical interventions to slow or stop cachexia progression. The purpose this study was to determine the role of skeletal muscle gp130 and STAT3 signaling in the regulation of cachexia induced muscle atrophy and mitochondrial loss. Specific …

Gestational Weight Gain And The Risk Of Obesity Among Preschool Children: Is This Mediated Through Birth Weight?, Nomathamsanqa Louise Mgutshini

Theses and Dissertations

Data from the 2001 Early Childhood Longitudinal Study Birth- Cohort were used to examine the association between maternal gestational weight gain (GWG) and the risk of obesity in the offspring, and the possible mediating role of birth weight. The Centers for Disease Control growth 2000 reference charts and child's height and weight measured at age 4 or 5 years old were used to determine child body mass index (BMI) Z-scores or obesity status (greater than or equal to the 95th percentile). Multiple linear or logistic regression models were used to adjust for maternal age, race, education, smoking status and prepregnancy …

John Pecham On Life And Mind, Caleb G. Colley

Theses and Dissertations

No abstract provided.

The Impact Of Insulin Management On Quality Of Life In Type 2 Diabetes, Sandra Ellen Mccormick

Dissertations

Complications of type 2 diabetes (T2DM) are severe but can be minimized through excellent glycemic control, optimally achieved by using intensive, or basal-bolus, insulin management. The quality of life (QOL) effects of basal-bolus insulin management are not fully known. This cross-sectional, observational study was based on the Revised Wilson and Cleary Model for Health Related Quality of Life, which measures five QOL domains (biological function, symptoms, functional status, general health perceptions, and overall QOL). The study aims were to describe, compare, and predict QOL in persons with T2DM based on type of insulin management (oral meds only, basal insulin only, …

Predicting Emotion Regulation In Early Childhood: The Impact Of Maternal Well-Being, Infant Crying, And Dyadic Mutuality, Tiffany Burkhardt

Dissertations

Learning adaptive emotion regulation skills in early childhood has been identified as fundamental to social competence, academic success, and psychological well-being. Because children learn to regulate their emotions through interactions with their caregivers, dyadic mutuality between the mother and infant may influence child emotion regulation capacity more than maternal behavior alone. To better understand the impact of maternal well-being and infant crying on the development of emotion regulation, parenting stress, maternal self-efficacy, maternal depression, and infant crying were examined with dyadic mutuality in the parent-child interaction to predict emotion regulation capacity.

A racially and socioeconomically diverse community sample of 149 …

Unveiling The Impact Of Human Influence On Species Distributions In Vietnam: A Case Study Using Babblers (Aves: Timaliidae), Laurel R. Yohe, Johnathan Flanders, Hoang Minh Duc, Long Vu, Sushma Reddy

Biology: Faculty Publications and Other Works

As developing countries give priority to economic growth, the effects of development threaten natural habitats and species distributions. Over the course of two decades, Vietnam has rapidly developed, especially in the expansion of agricultural production. However, no study has quantitatively measured the effects of recent human impact on the effects of past species distributions in Vietnam. We use locality data collected from multiple natural history collections, including several in Vietnam, to infer past species distributions. We assess habitat availability of five common babbler species (Aves: Timaliidae) using distribution models with data prior to rapid development that followed political reform. Overlaying …

The Effect Of Dietary Fat Intake On Hepatic Gene Expression In Lg/J And Sm/J Mice, Charlyn G. Partridge, Gloria L. Fawcett, Bing Wang, Clay F. Semenkovich, James M. Cheverud

Biology: Faculty Publications and Other Works

Background

The liver plays a major role in regulating metabolic homeostasis and is vital for nutrient metabolism. Identifying the genetic factors regulating these processes could lead to a greater understanding of how liver function responds to a high-fat diet and how that response may influence susceptibilities to obesity and metabolic syndrome. In this study we examine differences in hepatic gene expression between the LG/J and SM/J inbred mouse strains and how gene expression in these strains is affected by high-fat diet. LG/J and SM/J are known to differ in their responses to a high-fat diet for a variety of obesity- …

Structured Voluntary Youth Activities And Positive Outcomes In Adulthood: An Exploratory Study Of Involvement In Scouting And Subjective Well-Being, Sung Joon Jang, Byron R. Johnson, Young-Il Kim, Edward C. Polson, Buster G. Smith

Faculty Publications - Department of Sociology

This study explores whether youth involvement in Scouting has positive consequences later in life. We examine whether the number of years of participation in Scouting is positively associated with human and social capital and recreational lifestyles in adulthood, and whether these are linked to subjective well-being: relational, emotional, and physical health. To explore this potential relationship, we estimated a structural equation model, analyzing data from a national sample of adult males. We found that youth involvement in Scouting is positively related to subjective well-being indirectly via the positive adult outcomes.

Effectiveness Of A Closed System Device In Reducing Occupational Exposure And Environmental Concentrations Of Anticancer Drugs, Nitin Vyas

School of Geography, Earth and Environmental Sciences Theses

Owing to their non-selective nature, anti-cancer drugs affect both cancerous and non-cancerous cells and present a major health risk to healthcare staff working with them. This project was conducted at Derriford Hospital, Plymouth, to investigate the extent of contamination with anti-cancer drugs on work surfaces and the environmental emissions of these drugs. In the Isolator study, surface contamination arising from the preparation of five anticancer drug infusions (epirubicin, fluorouracil, cisplatin, oxaliplatin and carboplatin) in a pharmaceutical isolator and external surfaces of infusion bags and syringes using a conventional syringe and needle technique was investigated and compared with that obtained using …

Application Of Polychlorinated Biphenyl Signatures For Environmental Fingerprinting, David Peter Megson

School of Geography, Earth and Environmental Sciences Theses

Polychlorinated Biphenyls (PCBs) are a group of 209 ‘man-made’ chlorinated organic compounds that were widely used in the 20th century for a variety of industrial uses. PCBs were first commercially produced in the 1929 and were manufactured until the 1980s when their use was phased out due to environmental and human health risks. However, due to their widespread use and persistence they are ubiquitous in the environment and remain a contaminant of concern. The structural properties that determine the persistence of PCBs in humans were therefore elucidated by statistical analysis of data from the National Health and Nutrition Examination Survey …

Foundations In Wisconsin: A Directory [33rd Ed. 2014], Mary C. Frenn, Katie M. Barnhart, Brittany Carloni, Hilary Dabney, Alyssa Gruber, Jakob K. Rinderknecht

Foundations in Wisconsin: A Directory

The 2014 release of Foundations in Wisconsin marks the 33^rd edition of the print directory and the 14^th year of the online version. The directory is designed as a research tool for grantseekers interested in locating information on private, corporate, and community foundations registered in Wisconsin. Each entry in this new edition has been updated or reviewed to provide the most current information available. Most of the data was drawn from IRS 990-PF tax returns filed by the foundations. Additional information was obtained from surveys, foundation websites, annual reports, and newsletters.

The Biogeochemistry Of Iron, Zinc And Cobalt In The Atlantic Ocean: The Atlantic Meridional Transect And Uk Geotraces Sections, Neil Wyatt

School of Geography, Earth and Environmental Sciences Theses

Between 40 % and 50 % of the Earth’s primary production occurs in marine environments, primarily by phytoplankton. The trace metal micronutrients iron, zinc and cobalt are known to exert a significant biological control on phytoplankton productivity by serving as essential active centres in enzymatic processes such as inorganic carbon, nitrogen and phosphorus acquisition. The distributions and biogeochemistries of iron, zinc and cobalt therefore, have the potential to impact upon the global carbon cycle and hence climate. This research involves investigations into the biogeochemical cycling of iron, zinc and cobalt in the Atlantic Ocean. Iron measurements were conducted during October …

Exploring The Patient Experience Of Changes In Appetite And Diet With Incretin Analogue Therapy, Rosamund May Paisey

Other Faculty of Health Theses

Incretin analogue therapies are a valuable recent treatment option for type 2 diabetes (T2D) as they can improve blood glucose control and aid weight loss. The way in which an individual recognises and responds to changes in satiety signals induced by these treatments may explain why individual response is variable. Purposive sampling of individuals with successful outcome of incretin analogue therapy (GLP-1) for T2D gathered a participant cohort 15 people with 37 years (448 months) combined experience of this treatment and 149 years of living with diabetes. The focus group data reported in this thesis, explores their experiences, the differing …

Development Of A Nigerian Fermented Maize Food “Akamu” As A Functional Food, Patience Chisa Obinna-Echem

Other Faculty of Science and Engineering Theses

Akamu is a lactic acid bacteria fermented cereal-based food that complements infant diets in most African countries. Uncontrolled fermentation increases the variability in quality and safety of akamu. This study was aimed at the controlled fermentation of akamu with selected lactic acid bacteria (LAB), investigation of the probiotic potential of the LAB and the effect of variation in production method on the product quality and sensory properties. PCR-DGGE analysis of traditional akamu samples revealed LAB community dominated by Lactobacillus fermentum, L. plantarum, L. delbrueckii subsp. bulgaricus and L. helveticus. Isolated yeasts were Candida tropicalis, C. albicans, Clavispora lusitaniae and Saccharomyces …

Quality And Shelf Life Of Pita And Tandoori Breads Supplemented With Three Novel Functional Ingredients, Yaseen Galali

Other Faculty of Science and Engineering Theses

Abstract: The interest for functional foods rich in dietary fibre (DF) and low in glycaemic response (GR) is steadily increasing. This is because DF could reduce GR of food products and protect and restrict diet-associated diseases which have become common in society. But DF rich products are not always high in quality and palatability. Therefore, more fibre-rich functional products need to be designed and developed with good quality, palatability and longer shelf life. A response surface methodology (RSM) was used to develop both pita and Tandoori breads rich in DF with good quality, palatability and longer shelf life. So, a …

An Investigation Of The Coherence Of Instrumental Accumulation, Using A Sculptural Methodology, Nicola Ann Gilmour

Other Faculty of Arts, Humanities and Business Theses

This thesis questions the coherence of modes of fabrication that introduce materials to a living context, in forms that resist the processes of biological change. In doing so this project explores the ideologies embedded in fabrication that have led to this current position. The implications of an accumulation of materials being recognized as an autonomous object, and treated as if they are detached from their environment are also expanded. The sculptural methodology used to undertake this investigation has used the feature of materiality and it’s behaviours, of both human fabrication and the living environment, as a means to explore processes …

The Bioavailability And Assimilation Of Dietary Zinc In Rainbow Trout (Oncorhynchus Mykiss), Daniel James Leeming

Other Faculty of Science and Engineering Theses

This study examines three possible methods for improving the digestibility and bioavailability of zinc to rainbow trout (Oncorhynchus mikiss). The first method was to examine the availability of the zinc utilisation from commonly used protein sources; the second was to assess the efficacy of the upstream use of an enzyme treatment of the raw materials; the third was to assess the use of organically complexed mineral supplements as opposed to the inorganic salts widely used at present. The first section indicated that the zinc from the soyabean meal was the most available (49.4%). The zinc digestibilities of the animal based …

The Effect Of Probiotics On Bacterial Human Skin Pathogens, Jasim Hussein Al-Attwani

Other Faculty of Science and Engineering Theses

Probiotic bacteria have been investigated in the prevention and treatment of various diseases and allergies. The current study was undertaken to determine the effect of eight probiotic Lactobacillus species against bacterial human skin pathogens using several techniques. Antimicrobial activity of lactobacilli against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Propionibacterium acnes was evaluated using lactobacilli broth cultures (BCB) and cell free supernatant (CFS). Antimicrobial activity was significantly greater with BCB compared with CFS especially for Lactobacillus plantarum and Lactobacillus acidophilus. Lactobacilli and pathogen aggregation, biofilm formation and adhesion to keratin were assessed. L. casei and L. plantarum were selected for …

Perceived Stress, Coping And Eating Behaviours In Maltese Adolescents: Developing An Effective Online Intervention, Daniela Cassola

School of Health Professions Theses

Prevalence rates of overweight and obesity in Maltese adolescents are amongst the highest in the world. Stress-induced eating and dysfunctional coping skills have been linked to overeating and obesity. This study was undertaken in two phases. Phase 1 examined the relationship between perceived stress, coping and eating behaviours in Maltese adolescents and devised a model to guide the development of an effective Internet-based intervention. Based on the findings, Phase 2 developed ACES – a novel online intervention for the reduction of perceived stress and emotional eating in Maltese adolescents – and assessed its feasibility. In Phase 1, cross-sectional data were …

Somatic Embryogenesis And Cryopreservation Of Cauliflower (Brassica Oleracea Var. Botrytis), Magda Al Shamari

Other Faculty of Science and Engineering Theses

Abstract Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg L-1 and Kinetin at 0.1 mg L-1 and 3% sucrose. The response of different explant types (cotyledon, hypocotyls and root) through callus induction and subsequent culture was determined. The best period for subsequent callus culture was 21 days. Continuous immersion in …

Effect Of Dietary Β-Glucan Supplementation On Growth, Intestinal Functionality And Disease Progression In Selected Cyprinids (Cyprinus Carpio And Danio Rerio), Holger Kühlwein

Other Faculty of Science and Engineering Theses

Three experiments were conducted to investigate the effects of dietary supplementation of a ß-(1,3)(1,6)-D-glucan derived from the yeast Saccharomyces cerevisiae, on growth performance parameters and intestinal functionality in mirror carp (Cyprinus carpio L.) and on progression of a chronic, pre-existing mycobacteriosis in zebrafish (Danio rerio). The 1st experiment revealed that mirror carp fed diets containing 1% and 2% ß-glucan showed significant improvements in growth performance compared to fish fed both the control and the 0.1% ß-glucan containing diet. Equally, fish fed diets supplemented with 1% and 2% ß-glucan displayed significantly higher infiltration of leukocytes into the epithelial layer of in …

Attractiveness Privilege : The Unearned Advantages Of Physical Attractivenesss, Kelsey P. Yonce

Theses, Dissertations, and Projects

In this theoretical investigation, I explore level of physical attractiveness as a characteristic that privileges people who are more attractive and oppresses those who are less attractive. I discuss the concept of privilege, societal standards of physical attractiveness, and the ways in which people who are more physically attractive are treated and perceived more positively by others. To examine this phenomenon more deeply, I introduce the theoretical perspectives of intersectionality and conflict theory. Intersectionality refers to the idea that different areas of privilege and oppression interact with each other in ways that create unique experiences of privilege and oppression for …

Using Verbal Autopsy To Measure Causes Of Death: The Comparative Performance Of Existing Methods, C.J.L. Mahende, R. Lozano, A.D. Flaxman, P. Serina, D. Phillips, A. Stewart, S.L. James, A. Vahdatpour, C. Atkinson, M.K. Freeman, S.L. Ohno, R. Black, S.M. Ali, A.H. Baqui, L. Dandona, E. Dantzer, G.L. Darmstadt, V. Das, U. Dhingra, A. Dutta, W. Fawzi, S. Gomez, B. Hernandez, R. Joshi, H.D. Kalter, A. Kumar, V. Kumar, M. Lucero, S. Mehta, Zul Premji, D. Ramirez-Villalobos, H. Remolador, I. Riley, M. Romero, M. Said, D. Sanvictores, S. Sazawal, V. Tallo, A. D. Lopez

Pathology, East Africa

Background: Monitoring progress with disease and injury reduction in many populations will require widespread use of verbal autopsy (VA). Multiple methods have been developed for assigning cause of death from a VA but their application is restricted by uncertainty about their reliability.

Methods: We investigated the validity of five automated VA methods for assigning cause of death: InterVA-4, Random Forest (RF), Simplified Symptom Pattern (SSP), Tariff method (Tariff), and King-Lu (KL), in addition to physician review of VA forms (PCVA), based on 12,535 cases from diverse populations for which the true cause of death had been reliably established. For adults, …