Digital Commons Network^™

Novel 3d Head And Neck Cancer Model To Evaluate Chemotherapeutic Efficacy, Kelly Morgan

Theses and Dissertations

HNSCC accounts for 7 percent of all new cancer occurrences. Despite currently available treatments, there continues to be a high mortality and recurrence rate in HNSCC. Well over 50 percent of all cancer patients receive chemotherapy as a standard treatment. However, only 5 percent of these cases have been shown to help with treatment of the disease. Formerly, two options were available for drug testing: in vivo animal models, and in vitro two-dimensional models. While in vivo models remain the most representative, their use is burdened by high costs, time constraints, and ethical concerns. 2D models are simple to use …

Therapeutic Efficacy Of Combination Of Mtor Inhibitors And Ampk Activators In Non-Small Cell Lung Cancer., Grinal Corriea

Theses and Dissertations

Pemetrexed (PTX), an antifolate drug, has been approved by the US FDA for first line therapy of mesothelioma and non-small cell lung cancer. In addition to its primary site of action on thymidylate synthase (TS), PTX also inhibits the second folate-dependent enzyme of purine biosynthesis aminoimidazolecarboxamide ribonucleotide formyltransferase (AICART). The accumulation of the substrate for AICART, ZMP, in PTX-inhibited cancer cells leads to activation of AMP-activated protein kinase (AMPK) with subsequent inhibition of mammalian target of rapamycin (mTOR) and hypophosphorylation of its downstream targets responsible for protein synthesis and cell proliferation. Inhibitors of mTORC1 like Rapamycin and its analogs (rapalogs) …

Investigating The Role Of The Synaptic Transcriptome In Ethanol-Responsive Behaviors, Megan A. O'Brien

Theses and Dissertations

Alcoholism is a complex neurological disorder characterized by loss of control in limiting intake, compulsion to seek and imbibe ethanol, and chronic craving and relapse. It is suggested that the characteristic behaviors associated with the escalation of drug use are caused by long-term molecular adaptations precipitated by the drug’s continual administration. These lasting activity-dependent changes that underlie addiction-associated behavior are thought, in part, to depend on new protein synthesis and remodeling at the synapses. It is well established that mRNA can be transported to neuronal distal processes, where it can undergo localized translation that is regulated in a spatially restricted …

Structural Studies Of Interferon Regulatory Factor 4: A Molecular Perspective Of Its Regulatory Mechanism, Soumya Govinda Remesh

Theses and Dissertations

Interferon (IFN) regulatory factor family member 4 (IRF4) is a transcription factor that serves specific roles in transcriptional regulation of IFN responsive genes and is indispensable in B- & T-cell differentiation. IRF4 like the other members of the family has two major domains- the N-terminal DNA binding domain (DBD) essential for its recognition and binding to the Interferon Stimulated Response Element DNA sequence and a C-terminal Interferon activation domain (IAD) thought to maintain IRF4 in an auto-inhibited inactive state and is also critical in its activation. A putative unstructured linker connects the DBD and IAD. Activation in most members of …

Studies Of The Middle Class, Scott S. Stanard

Theses and Dissertations

I create oil paintings and drypoints that bring attention to scenes of American middle class life. I present crowds of people engaged in community activities and festivals. This enables me to depict a cross section of the residents of a given region. I also create a counterpoint to these peopled gatherings by painting and drawing the exteriors of middle class homes. In my work, I attempt to imbue a sense of intrigue and pathos.

I work from candid photos that I take of people and their living spaces. In these photos, I modify and delete elements to optimize narrative and …

Sildenafil And Celecoxib Interact To Kill Breast Cancer Cells, Brittany Binion

Theses and Dissertations

Breast cancer is the second most commonly diagnosed cancer among American women and is responsible for the second highest number of cancer-related deaths. Targeted therapeutic agents sildenafil, a phosphodiesterase type 5 inhibitor, and celecoxib, a cyclooxygenase-2 inhibitor, have been used individually in conjunction with other chemotherapeutic agents to enhance cell killing in a variety of cancers. Sildenafil when combined with traditional chemotherapeutic drugs, such as the taxanes and anthracyclines, or celecoxib combined with traditional hormone therapies have been used to increase cytotoxicity and cell killing. The data presented here demonstrates that the novel combination of sildenafil and celecoxib work together …

Seeing The Sausage Made: How Compromise Works In Large Groups And Representative Bodies, James E. Crawford Jr.

Theses and Dissertations

Inspired by the lack of Congressional compromise during the 2013 federal shutdown, I explore how compromise works in large groups and representative bodies. An on-line survey, personal interviews, and a discourse analysis of the Congressional Record yield a diverse collection of data, including personal and public stories of compromise. I examine the stories and other data through an eclectic mix of contemporary scholarship, borrowing literary theory from the Russian critic Mikhail Bakhtin, socio-linguistic concepts from American linguist James Paul Gee, and moral philosophy from Israeli thinker Avishai Margalit. I also incorporate the work of political scientists Amy Gutmann and Dennis …

Repetition, Betsy J. Yost

Theses and Dissertations

Artist Statement

My work is about memory, and my awareness of the repetition of large and small events in my life. In my paintings, drawings, and mixed media I create rhythmic patterns with images and colors to imply a sense of recurrence. I often repeat imagery such as paper dolls or geometric and organic shapes in my work. I choose and repeat shapes and colors that remind me of events in my childhood and in my life as an adult. I see these multiple images as symbolic self-portraits which prompt memories of recurring events, such as waking up each morning …

Regulation Of Pancreatic Α And Β Cell Function By The Bile Acid Receptor Tgr5, Divya Prasanna Kumar

Theses and Dissertations

The discovery that bile acids act as endogenous ligands of the membrane receptor TGR5 and the nuclear receptor FXR increased their significance as regulators of cholesterol, glucose and energy metabolism. Activation of TGR5, expressed on enteroendocrine L cells, by bile acids caused secretion of GLP-1, which stimulates insulin secretion from pancreatic β cells. Expression of TGR5 on pancreatic islet cells and the direct effect of bile acids on the endocrine functions of pancreas, however, are not fully understood. The aim of this study was to identify expression of TGR5 in pancreatic islet cells and determine the effect of bile acids …

Nonfiction, Documentary And Family Narrative: An Intersection Of Representational Discourses And Creative Practices, Kristine T. Weatherston

Theses and Dissertations

Nonfiction, Documentary, and Family Narrative:
 An Intersection of Representational Discourses and Creative Practices explores the role of personal memory, family history, and inter-generational storytelling as the basis for making a nonfiction film. The film, American Boy, tells the story of my mother’s immigration to the United States after the failed Hungarian Revolution of 1956, opening a discussion of four generations of my family life in the context of historical events, exile, self re-invention, and identity formation. As a media producer and nonfiction author, I narrate my understanding of these events to my infant son, as a way of communicating …

Barriers And Facilitators To Infant Feeding Among Low-Income African American Women, Cecilia E. Barbosa

Theses and Dissertations

Objective The purpose of the mixed methods study was to compare barriers and facilitators encountered by low-income African American women who engaged in different infant feeding practices. Methods Using the positive deviance approach and modified Integrated Model as theoretical frameworks, the research began with a qualitative study that informed a survey examining intention, skills, and environmental factors differentiating women’s infant feeding practices. Results Twenty-eight and 190 low-income African American adult women participated in focus groups or interviews and a survey, respectively. In the qualitative study, positive deviants, who breastfed for at least four months, seemed to have stronger breastfeeding intentions, …

Video Games: Their Effect On Society And How We Must Modernize Our Pedagogy For Students Of The Digital Age, Christopher J. Baker

Theses and Dissertations

This thesis aims to explore how video games have become an extremely beneficial tool in regards to education, art, medicine, psychology, economics, and beyond. Chapter 1 focuses on how ubiquitous video games have become in America, and what makes video games such a uniquely enjoyable experience to warrant such popularity. Chapter 2 explores how video games have become instrumental in various fields. Chapter 3 discusses the role that video games now play in the world of education; specifically how we, as educators, must adapt a modern pedagogy best suited to students who have grown up with video games, which have …

Vanadium Complexes Are In Vitro Inhibitors Of Leishmania Secreted Acid Phosphatases, Richard Mendez, Benjamin M. Dorsey, Craig C. Mclauchlan, Matt Beio, Timothy L. Turner, Victoria H. Nguyen, Alexander Su, William Beynon, Jon A. Friesen, Marjorie A. Jones

Faculty Publications – Chemistry

Leishmaniasis is a parasitic disease caused by the protozoa Leishmania. These organisms secrete acid phosphatases during their growth cycle as an important part of cell targeting to host macrophage cells thus allowing for a successful infection. Secreted acid phosphatases (SAP) are reported to play a significant role in the survival of Leishmania cells, thus evaluation of these enzymes is of interest. The inhibition of SAP can be the focus of a new drug therapy. We tested for SAP activity from Leishmania tarentolae following the addition of a series of vanadium complexes including decavanadate. Cell cultures at different stages in their …

Experimental Development Of Advanced Air Filtration Media Based On Electrospun Polymer Fibers, Negar Ghochaghi

Theses and Dissertations

Electrospinning is a process by which polymer fibers can be produced using an electrostatically driven fluid jet. Electrospun fibers can be produced at the micro- or nano-scale and are, therefore, very promising for air filtration applications. However, because electrospun fibers are electrically charged, it is difficult to control the morphology of filtration media. Fiber size, alignment and uniformity are very important factors that affect filter performance. The focus of this project is to understand the relationship between filter morphology and performance and to develop new methods to create filtration media with optimum morphology.

This study is divided into three focus …

Fabrication And Characterization Of High Surface Area Gold Electrodes, Madhura S. Damle

Theses and Dissertations

High surface area gold electrodes are very good substrates for biosensors, catalysis and drug delivery. Their performance is characterized by good sensitivity, low detection limit and high signal. As a result, extensive research is being carried out in this field using different approaches of fabrication to generate high surface area porous electrodes of different morphology, pore size and structure. The morphology of the electrodes can be changed based on whether the approach involves a template or not, types of metal deposition, method and time of dealloying etc. The deposition of metal can be carried out using various approaches such as …

Visualization, Viewer And Emotion: An Empirical Study Of Cognition And Affective Responses To Infographics Used For Crisis Communication, Sean M. Stewart

Theses and Dissertations

A 3 (crisis response strategy) x 3 (medium) factorial design experiment was conducted to determine if information graphics conveyed through online news sources may be more beneficial for organizational reputations during some crisis situations than the use of news videos and text-based news stories. Variables examined include cognitive and affective appraisal, third-person effect, behavioral response and crisis response strategy.

Recent research in organizational crisis communications has pointed to the fact that more information is needed regarding how individuals react cognitively and affectively to crisis communications. Current crisis communications literature is also sparse concerning the behavioral aspects of crisis message reception …

Nf-Κb P50: The Role Of Redox Signaling And Loss Of Function In Dysregulated Microglial Activation, Thomas Taetzsch

Theses and Dissertations

Abstract

NF-κB p50: THE ROLE OF REDOX SIGNALING AND LOSS OF FUNCTION IN DYSREGULATED MICROGLIAL ACTIVATION

By Thomas J Taetzsch B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.

Virginia Commonwealth University, 2014

Major Director: Michelle L Block PhD,

IUPUI Associate Professor

Department of Anatomy and Neurobiology

Oxidative stress and chronic microglia-mediated inflammation are well characterized underlying factors in neurodegenerative disease. While it is clear that increased levels of inflammation and augmented oxidative stress in the aged brain create an environment that is particularly harmful to neurons, little …

Capillarity And Two-Phase Fluid Transport In Media With Fibers Of Dissimilar Properties, Thomas M. Bucher Jr.

Theses and Dissertations

Capillarity is a physical phenomenon that acts as a driving force in the displacement of one fluid by another within a porous medium. This mechanism operates on the micro and nanoscale, and is responsible for countless observable events. This can include applications such as absorption in various hygiene products, self-cleaning surfaces such as water beading up and rolling off a specially-coated windshield, anti-icing, and water management in fuel cells, among many others.

The most significant research into capillarity has occurred within the last century or so. Traditional formulations for fluid absorption include the Lucas–Washburn model for porous media, which is …

Lucky Strike House: The Space Between, Monika Shreves

Theses and Dissertations

With the onset of one of the worst economic downturns in recent history, the face of poverty is changing. This project seeks to explore a design solution aimed at helping a newly emerging segment of the population: the “half homeless”, formerly middle class individuals/families who lost their jobs and homes during the recession.

Set in the building known as the Lucky Strike Power Plant, the project contains two distinct, but overlapping, programs: 1) multi-family housing, and 2) a continuum of supportive services accessible to both the residents and the community at large. The main focus of this project is the …

Transportation Safety In Virginia: Positive Changes And Future Prospects, Nouran Hakami

Theses and Dissertations

Measured by the level of transportation safety, the Commonwealth of Virginia stands out from all the States because despite increased need for mobility, it manages to maintain its safety indices at exceptionally good levels. In many respects we can attribute this success to the comprehensive Strategic Highway Safety Plan of Virginia (SHSP), which is, as concluded from the analysis of its analogues, among the best in the US. The programs and policies described in this document embrace all aspects of transportation safety and create a harmonious system. To assess the effectiveness of the SHSP, this thesis used correlation and regression …

Where Does Electronic Aggression Fit?: A Comparison Of Dimensional And Categorical Models Of Adolescent Aggression, Krista R. Mehari

Theses and Dissertations

Electronic aggression is a rapidly growing focus of research, but it lacks a unifying theoretical framework that is necessary to advance the field. The lack of a theoretical framework has led to inconsistencies in measurement of electronic aggression, making it difficult to draw conclusions across studies. In general, researchers have assumed that electronic aggression constitutes a new form of aggression, a counterpart to physical, verbal, and relational aggression, due to unique features surrounding the perpetration of electronic aggression. Furthermore, researchers have treated electronic aggression as a categorical variable based on the assumption that “cyberbullies” constitute a distinct group of adolescents. …

Test Development To Determine The Feasibility Of Rubber Tractor Treads For Use As Blasting Mats, Matthew Kurtis Coy

Masters Theses

"The objective of this thesis was to determine if rubber tractor treads could be used as an alternative material for blasting mats. These treads are made from steel cable and vulcanized rubber and are very tough. However, once they reach the end of their life on mobile equipment, they are destined for a landfill, or dump site because they are too tough to recycle. A potential alternative use for them is as blasting mats.

An examination of existing mat designs was conducted to better understand the matting methods used by the blasting industry today, to learn from those designs allow …

Eugene L. Barnwell Papers - Accession 1011, Eugene Lindsay Barnwell

Manuscript Collection

The Eugene L. Barnwell Papers consist of correspondence, newspaper clippings, speeches, training materials, memorabilia, and personal reflections concerning his life and careers in music and public service including his service as mayor of York, SC from 1982-1991. Of particular interest among the Barnwell Papers is his study of municipal management relative to a council/manager form of government.

Unloosed: Designing Participatory Objects, Laura Schlifer

Theses and Dissertations

The ubiquity of technology has mediated the means of receiving content through digital networks; users have complete control over receiving, shaping, and sharing information. In contrast to the inherent elasticity of these networks, physical pieces of communication often manifest through a closed and highly controlled process. However, the increased prominence of user-interaction with media provides an opportunity to evaluate the design process as it applies to the creation of physical objects. Throughout much of my work, I investigate the potential for unloosening the control of designed objects by inviting others into the design process. By considering the audience as active …

Side A, Side B, Hong Zhang

Theses and Dissertations

I believe that aural and visual language can contribute and inspire one another. Sound evokes the emotions and the senses. It is a catalyst for our imagination. It can be combined with imagery for the purpose of communication, harmoniously or contrastively. Both have corresponding characteristics—color/timbre, brightness/pitch, length/duration, scale/volume, composition/rhythm—and thus provide ways of transforming each other back and forth as input and output. Computer technology has increased the ease of transforming and manipulating the two. In my thesis project, I explore sound visualization, sound/image transformation and combination.

Designing Connection, Laurie K. Culshaw

Theses and Dissertations

Social connection is an essential human need. Personal connections exist at a variety of depths and within different types of relationships. Small daily choices determine the strength of those connections and their impact on our well-being as individuals and as a community. Modern society and technology have altered the speed and channels of connection, increasing communication but decreasing meaningful connection. It is critical to understand how the methods of communication affect the depth of connections. Through a series of participatory graphic design projects, I analyze the strengths and weaknesses of one-on-one, small group and community connections within an established taxonomy …

Epigenetic Alterations Of Toll-Like Receptors By Tet2 In Spontaneous Preterm Labor, Anuja Chumble

Theses and Dissertations

Increasing evidence implicates the presence of bacteria in intrauterine tissues as an important risk factor for spontaneous preterm labor. Epigenetic alterations of innate immunity genes may increase the mother’s sensitivity to subclinical levels of bacteria. This study examined the presence of TET2, TLR-2, and TLR-9 in intrauterine tissue, and evaluated whether epigenetic alterations of these genes, as well as IL-8, changed their expression in human decidual tissue and a macrophage cell culture. Immunohistochemicalstaining was used to detect the presence of these proteins in intrauterine tissue. Gene expression changes were evaluated in stimulated monocytes and macrophages. Fluorescence immunohistochemistry was used to …

The Copyright/Patent Boundary, Viva R. Moffat

University of Richmond Law Review

No abstract provided.

Lighting A Fire Under Free Speech: The Fda's Graphic Attempts To Reduce Smoking Rates, Ashley Peterson

University of Richmond Law Review

No abstract provided.

The Law And Economics Of Corporate Social Responsibility And Greenwashing, Miriam A. Cherry

Faculty Publications

In this symposium article, I explore the link between corporate social responsibility (CSR) and the threat of greenwashing. In the first part of the article, I start with first principles, examining the origins of greenwashing, structuring its definitions, and identifying the economic incentives that lead firms into the practice. The second part of this article examines the legal structure that allows greenwashing to occur, and with it, explores the pervasiveness and extent of greenwashing. The third part of this article articulates the harms of greenwashing. Intuitively, greenwashing involves deception, falsity, and hypocrisy that reflexively seem problematic. Identifying the actual harm …