Digital Commons Network^™

The Valley-Of-Death: Reciprocal Sign Epistasis Constrains Adaptive Trajectories In A Constant, Nutrient Limiting Environment, Kami E. Chiotti, Daniel J. Kvitek, Karen H. Schmidt, Gregory Koniges, Katja Schwartz, Elizabeth A. Donckels, Frank Rosenzweig, Gavin Sherlock

Biological Sciences Faculty Publications

The fitness landscape is a powerful metaphor for describing the relationship between genotype and phenotype for a population under selection. However, empirical data as to the topography of fitness landscapes are limited, owing to difficulties in measuring fitness for large numbers of genotypes under any condition. We previously reported a case of reciprocal sign epistasis (RSE), where two mutations individually increased yeast fitness in a glucose-limited environment, but reduced fitness when combined, suggesting the existence of two peaks on the fitness landscape. We sought to determine whether a ridge connected these peaks so that populations founded by one mutant could …

Hand Hygiene Promotion: An Essential Strategy For Preventing Foodborne Disease In Elementary Schools, Zhangqi Wang

All Theses

Student absenteeism is a pervasive problem in the United States, causing a number of educational, economic, and institutional problems. Hand-hygiene interventions have been reported to be a method for reducing illness-associated student absenteeism. As an increasing number of schools offer self-service, including salad bars and bowls of whole fresh fruit, opportunities for the transmission of foodborne pathogens via hands could possibly increase illness-associated student absenteeism. To address this problem, we conducted a two-phase study. First, we conducted a systematic literature review to evaluate peer-reviewed studies (N=24) that reported an association between hand hygiene interventions and illness-associated absenteeism reduction in elementary …

Characterization Of A Novel Fungal-Specific Gene, Fug1, In Fusarium Verticillioides, John Byron Ridenour

Graduate Theses and Dissertations

Filamentous fungi are responsible for numerous plant and animal diseases. The filamentous ascomycete Fusarium verticillioides is a globally important pathogen of maize, capable of causing severe yield reductions and economic losses. Also of substantial concern is the contamination of infected kernels with fumonisins, toxic secondary metabolites linked to toxicoses in humans and livestock. The number of sequenced fungal genomes is rapidly increasing. However, functional characterization of fungal genes has not progressed at a comparable rate. In pathogenic fungi, uncharacterized genes represent a source for novel virulence factors or anti-fungal targets. Therefore, to fully understand the genetic mechanisms underlying fungal pathogenesis, …

Dissection Of Gray Leaf Spot Of Maize Through Functional Genomics, Robert Louis Hirsch

Graduate Theses and Dissertations

Gray leaf spot, caused by Cercospora zeae-maydis, is a devastating disease of maize that reduces yields and increases management costs. C. zeae-maydis penetrates maize leaves through stomata, but the biological and molecular bases of this process are poorly understood. The goal of this research was to elucidate the biological parameters of stomatal infection in C. zeae-maydis, and to identify and characterize novel genetic pathways involved in stomatal sensing and pathogenesis. Histopathological observations of a GFP-expressing strain of C. zeae-maydis during infection of maize indicated that the fungus responded to host-derived stomatal cues during the infection process. C. zeae-maydis was observed …

A Large-Scale, Rapid Public Health Response To Rabies In An Organ Recipient And The Previously Undiagnosed Organ Donor, R. M. Wallace, Danielle Stanek, S. Griese, D. Krulak, Neil M. Vora, Virginia Kan, +14 Additional Authors

Medicine Faculty Publications

This article describes and contrasts the public health response to two human rabies cases: one organ recipient diagnosed within days of symptom onset and the transplant donor who was diagnosed 18 months post-symptom onset. In response to an organ-transplant-related rabies case diagnosed in 2013, organ donor and recipient investigations were conducted by multiple public health agencies. Persons with potential exposure to infectious patient materials were assessed for rabies virus exposure. An exposure investigation was conducted to determine the source of the organ donor's infection. Over 100 persons from more than 20 agencies spent over 2700 h conducting contact investigations in …

Whole Genome Sequencing Of A Methicillin-Resistant Staphylococcus Aureus Pseudo-Outbreak In A Professional Football Team., Deverick J. Anderson, Simon R. Harris, Eliot Godofsky, Todd Toriscelli, Thomas H. Rude, Kevin Elder, Daniel J. Sexton, Elliot J. Pellman, Thom Mayer, Vance G. Fowler, Sharon J. Peacock

Medicine Faculty Publications

Two American football players on the same team were diagnosed with methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infections on the same day. Our investigation, including whole genome sequencing, confirmed that players did not transmit MRSA to one another nor did they acquire the MRSA from a single source within the training facility.

Review Of "The Pku Paradox: A Short History Of A Genetic Disease" By D. B. Paul And J. P. Brosco, John B. Jenkins

Biology Faculty Works

No abstract provided.

Hiv Drug Resistance Among Infants And Children In South Africa: How Efficient Is Genotypic Testing?, Ellen Hendrix

Independent Study Project (ISP) Collection

Background: South Africa has the largest prevalence of HIV infection. This epidemic impacts adults as well as the pediatric population. The presence of drug-resistant mutations to antiretroviral therapies among infants and children is on the rise. Few studies have been conducted on this topic. Objective: The study aims to determine whether drug resistance testing in the form of genotypic testing is cost-effective when deciding whether to switch to a new HIV antiretroviral therapy following drug failure. Method: An interactive research approach is taken by collecting primary data from experts in this field. Secondary sources including guidelines from the World Health …

The Resistance Of A North American Bat Species (Eptesicus Fuscus) To White-Nose Syndrome (Wns), Craig L. Frank, Andrew Michalski, Anne A. Mcdonough, Marjon Rahimian, Robert J. Rudd, Carl Herzog

KIP Articles

No abstract provided.

Design, Synthesis, And Biological Evaluation Of Novel Taxoid-Based Drug Conjugates And Theranostic Imaging Agents Towards Tumor-Targeted Chemotherapy, Jacob G. Vineberg

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Cancer remains the second leading cause of death in the United States, accounting for nearly a quarter of all deaths, and is exceeded only by heart disease. Traditional chemotherapy uses highly potent cytotoxic agents to kill rapidly dividing cancer cells, relying on the premise that these fast-growing cancer cells are more likely to be killed by these drugs. Despite the profound impact of traditional chemotherapy, lack of tumor-specificity continues to be a serious issue for cancer treatment. Paclitaxel and docetaxel are among the most widely used chemotherapeutic agents, in particular against cancers such as ovarian, breast, lung, and Kaposi's sarcoma; …

The Influence Of Drought On The Ecological And Evolutionary Genomics Of Fungal Pathogenesis In An Annual Plant, Brassica Rapa, Niamh Breda O'Hara

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

There is growing evidence that populations are evolving rapidly in response to climate change. However, the potential cost associated with adapting, and the genetic basis of both rapid evolution and costs of adaptation remain largely unexplored. With climate change, drought is increasing in frequency and severity, widely affecting plant yield and fitness in agricultural and natural populations. For my dissertation, I integrated multiple approaches (field, greenhouse, lab and bioinformatic) to explore the ecological and evolutionary effects of drought on an agriculturally important plant-pathogen system: the annual herbaceous plant Brassica rapa (field mustard), and its fungal pathogen Alternaria brassicae. In a …

Part I. Design, Synthesis And Biological Evaluation Of Novel 2,5,6-Trisubstituted Benzimidazoles Targeting Ftsz As Antitubercular Agents., Part Ii. Development Of Novel Taxoid-Based Drug Conjugates And Theranostic Imaging Agents Towards Tumor-Targeted Chemotherapy, Bora Park

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Part I. Design, synthesis and biological evaluation of novel 2,5,6-trisubstituted benzimidazoles targeting FtsZ as antitubercular agents Filamenting temperature-sensitive protein Z (FtsZ), an essential cell division protein, is a promising target for the drug discovery of new-generation antibacterial agents against various bacterial pathogens. As a part of SAR studies on benzimidazoles, we have synthesized a library of 376 novel 2,5,6-trisubstituted benzimidazoles, bearing ether or thioether linkage at the 6-position. In a preliminary HTP screening against Mtb H37Rv, 108 compounds were identified as hits at a cut off values of 5 ìg/mL. Among those hits, 10 compounds exhibited MIC values in the …

Modeling Colony Collapse Disorder In Honeybees As A Contagion, Christopher Kribs, Christopher Mitchell

Mathematics Faculty Publications - Archive

Honeybee pollination accounts annually for over $14 billion in United States agriculture alone. Within the past decade there has been a mysterious mass die-off of honeybees, an estimated 10 million beehives and sometimes as much as 90% of an apiary. There is still no consensus on what causes this phenomenon, called Colony Collapse Disorder, or CCD. Several mathematical models have studied CCD by only focusing on infection dynamics. We created a model to account for both healthy hive dynamics and hive extinction due to CCD, modeling CCD via a transmissible infection brought to the hive by foragers. The system of …

Uk Ag Equine Programs 2015 Calendar: A 12-Month Planning Calendar For The Care And Use Of Your Horses, Jason Phillips, Glenn Mackie, Don Sorrell, Nicholas L. Carter, Paula A. Jerrell, Brian Newman, Daniel Wilson, Christopher Jeffcoat, Karen Douglas, Katheryn L. Cerny, Fernanda Camargo, Robert Coleman, Essie Rodgers, Jim Smith

Agriculture and Natural Resources Publications

The information in this calendar is provided to aid owners in planning for the care and use of their horses. When necessary, information is discussed in the month prior to application to allow horse owners adequate time to plan for activities such as weed control, soil testing, and vaccinations.

Contact your local veterinarian for health-related issues and your county extension agent for further information. Phone numbers are listed at the end of the calendar.

Rnase L Contributes To Experimentally Induced Type 1 Diabetes Onset In Mice, Chun Zeng, Xin Yi, Danny Zipris, Hongli Liu, Lin Zhang, Qiaoyun Zheng, Krishnamurthy Malathi, Ge Jin, Aimin Zhou

Chemistry Faculty Publications

The cause of type 1 diabetes continues to be a focus of investigation. Studies have revealed that interferon α (IFNα) in pancreatic islets after viral infection or treatment with double-stranded RNA (dsRNA), a mimic of viral infection, is associated with the onset of type 1 diabetes. However, how IFNα contributes to the onset of type 1 diabetes is obscure. In this study, we found that 2-5A-dependent RNase L (RNase L), an IFNα-inducible enzyme that functions in the antiviral and antiproliferative activities of IFN, played an important role in dsRNA-induced onset of type 1 diabetes. Using RNase L-deficient, rat insulin promoter-B7.1 …

Surface Modification Of Adenovirus Type 5 Vectors Via Two-Step Metabolic Labeling For Pet Imaging, Jai Inder Pal

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Surface modification of viruses is of great interest for therapeutic gene delivery. Numerous viruses have been, and continue to be engineered in an attempt to optimize them for targeted in vivo gene therapy. Adenovirus has been shown by a myriad of publications to be the most efficient delivery vehicle in different cell types. But while it is the preferred delivery agent in many gene therapy studies, drawbacks such as off-targeting, immune response and toxicity still hinder it from being used in clinical studies. In an attempt to modify the surface of Adenovirus efficiently, our lab developed a simple two-step labeling …

Regulation Of C-Reactive Protein Gene Expression And Function, Avinash N. Thirumalai

Electronic Theses and Dissertations

Human C-reactive protein (CRP) is the prototypic acute phase protein whose serum concentration increases rapidly during inflammation. CRP is also associated with atherosclerosis; it is deposited at lesion sites where it may interact with modified lipoproteins. There are 2 major questions regarding CRP: 1. How is the serum concentration of CRP regulated? 2. What are the functions of CRP in atherosclerosis?

Our first aim was to determine the role of the constitutively expressed transcription factor Oct-1 in regulating CRP gene expression. We found that Oct-1 overexpression inhibited (IL-6+IL-1β)- induced CRP gene expression; maximal inhibition required the binding of Oct-1 to …

Top 10 Medicinal Plants And Their Groundbreaking Impact On Modern Medicine, Jordan N. Patterson, Joan Marie Lausier

Senior Honors Projects

This paper describes ten plants that having been groundbreaking drugs in the past century that were all derived from plants. I chose to do my project on this topic because of my family’s history with natural medicine. In 1999 my mother was diagnosed with Bile Duct cancer and the doctors gave her only a couple of weeks to live. The doctors recommended chemotherapy and radiation, but the results seemed negative for the situation. After some extensive research, my parents found St. Georges Medicinal Wellness Center located in the tiny village of Bad Aibling, Germany. This hospital along with offering traditional …

Making Dioramas Of Women Scientists Help Elementary Students Recognize Their Contributions, Jolene K. Teske, Phyllis Gray, Julie L. Klein, Audrey C. Rule

Curriculum & Instruction Faculty Publications

The STEM movement encourages girls to consider careers in science; however, for success, common misconceptions and biases need to be dispelled, while females’ spatial thinking skills are developed. All students, both girls and boys, need exposure to the accomplishments of women scientists to appreciate their contributions and to envision females as successful scientists. This one-week study conducted during a summer day camp examined upper elementary student (n = 15; 7 females, 8 males) attitudes toward science, women in science, and the possibility of a science career before and after participation in learning about diverse accomplished women scientists and making a …

Utilizing Landsat Tm And Oli In Predicting Oncomelania Hupensis Habitats Around Poyang Lake Before And After Three Gorges Dam Completion, Stephanie L. Mccracken

Honors Theses

Schistosomiasis is a parasitic disease caused by the Schistomsoma japonicum flatworm that utilizes the Oncomelania hupensis snail as an intermediate agent. In the People’s Republic of China, these amphibious snails contaminate freshwater systems infecting humans, bovines, and other mammals and have caused significant morbidity for over two thousand years (Wertheim, et al. 2012; Zhang, et al. 2012.) The gravity of this disease prompted the national government to initiate sizable public health programs, such as the World Bank Loan Project (WBLP.) In spite of WBLP's achievements, in 2004, after this program ended, a national survey acknowledged a resurgence of schistosomiasis in …

Nurse Knowledge Of Clostridium Difficile, Ashley E. Frosch

Honors Theses

The purpose of this study is to determine the amount of knowledge nurses have about Clostridium difficile. Seventy-five nurses participated in this study. A 25-question true/false survey was distributed via email to graduates of The University of Southern Mississippi. The survey consisted of a brief demographic section and 25 questions addressing Clostridium difficile knowledge. A total of 582 surveys were sent out with a total of 75 completed. Out of 25 questions in the survey, the majority of respondents (>50%) only answered 7 questions incorrectly. Overall, the nurses in this study possessed accurate knowledge about C. difficile; although, there …

Obesity Among Young American Indian Adults In North Dakota, Abby Omahen

All-Inclusive List of Electronic Theses and Dissertations

Obesity among American Indian young adults is a significant health problem. There is an increase in the prevalence rate of diseases associated with obesity which tend to have high medical costs and high mortality rates. Fruit and vegetable consumption, exercise, gender and education are factors which could affect obesity among American Indians. Therefore, the primary purpose of the current study was to determine if there was an association between certain factors (fruit and vegetable consumption, exercise, gender and education level) and obesity among young American Indian adults in North Dakota. The secondary purpose was to determine if there was a …

Development Of A Turkey Coccidiosis Vaccine Candidate: An Attenuated Line Of The Protozoan Parasite, Eimeria Meleagrimitis, Thilakar Rathinam

Graduate Theses and Dissertations

Coccidiosis is an economically important enteric disease of poultry caused by protozoan parasites belonging to the genus Eimeria. Drugs are most commonly used to control coccidiosis. Widespread usage of anticoccidial drugs in the field has resulted in development of drug resistant strains and consequently decreased drug efficacy. An alternative control strategy using attenuated vaccines is desired. A series of experiments was designed to identify, isolate and attenuate a strain of a common and pathogenic species of Eimeria of turkeys, E. meleagrimitis.

Sensitivity of field isolates of turkey coccidia to the anticoccidials drugs amprolium, clopidol, diclazuril, and monensin was investigated. A …

Antibacterial Activity Of Commercially Available Plant Extracts On Selected Campylobacter Jejuni Strains, Divya Oppath Sivasankaran

Graduate Theses and Dissertations

Campylobacter jejuni is recognized as a leading cause of foodborne illness worldwide with approximately 850,000 cases per year in the United Sates. A total of 18 species of Campylobacter have been identified worldwide to date. Foodborne transmission of C. jejuni is mainly through the consumption of unpasteurized milk, contaminated poultry meat and water. C. jejuni can survive in very diverse environments under stressful conditions in human and animals which is different from the specific conditions and media required in laboratory experiments. There has been increased research in recent years to identify naturally occurring antibacterial agents to control and eliminate C. …

Development And Characterization Of An Autologous Whole Cell Breast Cancer Vaccine, Samantha Leigh Kurtz

Graduate Theses and Dissertations

Approximately 40,000 women will die from breast cancer in the United States in 2014. About 90% of these deaths will be due to metastases, rather than the primary tumor and majority of metastases are due to the recurrence and progression of non-metastatic disease. Current adjuvant treatments, such as chemotherapy and radiation, have severe side effects and may result in overtreatment and drug resistance.

Since greater than 90% of patients are diagnosed between stages I-III and have minimal residual disease after treatment, there is an opportunity to treat patients with an autologous breast cancer vaccine. Autologous vaccines under development have a …

Acanthamoeba Spp. As Reservoirs For Transmission Of Norovirus, Tun-Yun Hsueh

Graduate Theses and Dissertations

Human noroviruses (HuNoV) are the most common cause of foodborne disease outbreaks in the United States and the most common food commodities implicated in HuNoV outbreaks are leafy greens; however, the vehicle of transmission and point of contamination are often unknown. Here, we hypothesize that common free-living amoebae (FLA) ubiquitous in the environment may act as reservoirs of HuNoV and facilitate the transmission of these pathogens to fresh produce. The objective of this research was to first evaluate the interaction/association between HuNoV surrogates and Acanthamoeba by incubating them together and analyzing virus titer associated with amoeba through an 8 day …

The Current Prevalence Of Caprine Arthritis-Encephalitis Virus In Midwestern Goat Herds, Bradley Todd Jones

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Caprine Arthritis-Encephalitis Virus (CAEV) is an incurable disease of goats that has both social and economic impacts. Clinical disease in goats includes encephalitis in kids; chronic arthritis, inflammatory mastitis and progressive respiratory disease in adults. In the last 25 years there have been significant changes in the United States goat industry with rapid growth in the meat and dairy industries. Recent prevalence studies are lacking and historic studies may not reflect changes in the industry. The purpose of this study was to establish the prevalence of CAEV in Midwestern herds that are not routinely acquiring new animals from known negative …

Issues Of Social Data Analytics With A New Method For Sentiment Analysis Of Social Media Data, Zhaoxia Wang, Victor J. C. Tong, David Chan

Research Collection School of Social Sciences

Social media data consists of feedback, critiques and other comments that are posted online by internet users. Collectively, these comments may reflect sentiments that are sometimes not captured in traditional data collection methods such as administering a survey questionnaire. Thus, social media data offers a rich source of information, which can be adequately analyzed and understood. In this paper, we survey the extant research literature on sentiment analysis and discuss various limitations of the existing analytical methods. A major limitation in the large majority of existing research is the exclusive focus on social media data in the English language. There …

Compulsory Water Fluoridation: Justifiable Public Health Benefit Or Human Experimental Research Without Informed Consent?, Rita Barnett-Rose

William & Mary Environmental Law and Policy Review

No abstract provided.

Rethinking Ddt: The Misguided Goals Of The Stockholm Convention On Persistent Organic Pollutants And A Plan To Fight Malaria Worldwide, Eva Zelson

William & Mary Environmental Law and Policy Review

No abstract provided.