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Simple, Label-Free And Non-Instrumented Analyte Quantitation By Flow Distance Measurement In Microfluidic Devices, Debolina Chatterjee

Theses and Dissertations

Rapid determination of the concentrations of molecules related to diseases can provide timely information for treatment options. However, most biomarker quantitation methods require costly and complex equipment. On the other hand, point-of-care systems have less complex instrumentation needs than laboratory-based equipment, but often provide less information; for example, biomarker presence or absence instead of concentration. A complete analysis setup addressing key limitations of both laboratory-based and portable systems is highly desirable. I developed microfluidic devices with visual inspection readout of a target’s concentration from microliter volumes of solution flowed into a microchannel. Microchannels are formed within polydimethylsiloxane (PDMS), and the …

The Microtubule-Associated Protein, Eb1, Links Aim2 Inflammasomes With Autophagy-Dependent Secretion, Li-Jie Wang, Hsin-Yi Huang, Meng-Pin Huang, Willisa Liou, Ya-Ting Chang, Chih-Ching Wu, David M. Ojcius, Yu-Sun Chang

All Dugoni School of Dentistry Faculty Articles

Inflammasomes are multi-protein complexes that regulate chronic inflammation-associated diseases by inducing interleukin-1 β (IL-1β) secretion. Numerous components involved in inflammasome activation have been identified, but the mechanisms of inflammasome-mediated IL-1β secretion have not yet been fully explored. Here, we demonstrate that end-binding protein 1 (EB1), which is required for activation of AIM2 inflammasome complex, links the AIM2 inflammasome to autophagy-dependent secretion. Imaging studies revealed that AIM2 inflammasomes colocalize with microtubule organizing centers and autophagosomes. Biochemical analyses showed that poly(dA-dT)-activated AIM2 inflammasomes induce autophagy and IL-1β secretion in an LC3-dependent fashion. Furthermore, depletion of EB1 decreases autophagic shedding and intracellular trafficking. …

Water, Behavior, And Health In Alaska, Troy L. Ritter

Biological Sciences

This dissertation addresses the need for a better understanding of how water and sanitation infrastructure and water use behaviors come together to influence health. The ultimate aim is to inform water infrastructure designs and behavior change programming for the prevention of acute respiratory infections (ARIs), skin infections, and diarrhea. All three diseases are of public health significance in Alaska, and all three can be prevented by proper access and use of water and sanitation services. I begin the dissertation by illustrating that some residents who have access to treated water continue to consume untreated river water and rain. In fact, …

Animal Health Matters, Russ Daly, Jane Hennings

Animal Health MATTERS Newsletter

[Page] 1- ADRDL Welcomes Pathologist Pillatzki [Page] 1- More New Hires to Start Soon at VBS Department [Page] 2- Director's Message: Addressing Critical Needs of the ADRDL—A White Paper [Page] 3- Reporting Requirements for Swine Enteric Coronaviruses: What They Mean for Swine Producers and their Veterinarians [Page] 4- Histopathology and the Histology Laboratory at SDSU’s ADRDL [Page] 5- Pre-Veterinary Students Honored by SDVMA and SDSU Through Stethoscope Ceremony [Page] 6- New OPP and CAE Tests to be Offered “One Health” Workshop Group Meeting [Page] 6- New Hires [Page] 6- ADRDL Bacteriologist Serves as President [Page] 6- ADRDL Case Reports at …

Incidence And Trends Of Blastomycosis-Associated Hospitalizations In The United States, Amy E. Seitz, Naji Younes, Claudia A. Steiner, Rebecca Prevots

Epidemiology Faculty Publications

We used the State Inpatient Databases from the United States Agency for Healthcare Research and Quality to provide state-specific age-adjusted blastomycosis-associated hospitalization incidence throughout the entire United States. Among the 46 states studied, states within the Mississippi and Ohio River valleys had the highest age-adjusted hospitalization incidence. Specifically, Wisconsin had the highest age-adjusted hospitalization incidence (2.9 hospitalizations per 100,000 person-years). Trends were studied in the five highest hospitalization incidence states. From 2000 to 2011, blastomycosis-associated hospitalizations increased significantly in Illinois and Kentucky with an average annual increase of 4.4% and 8.4%, respectively. Trends varied significantly by state. Overall, 64% of …

Big Data In Science And Healthcare: A Review Of Recent Literature And Perspectives, Margaret M. Hansen Edd, Msn, Rn, T Miron-Shatz, Ays Lau, C Paton

Nursing and Health Professions Faculty Research and Publications

Objectives: As technology continues to evolve and rise in various industries, such as healthcare, science, education, and gaming, a sophisticated concept known as Big Data is surfacing. The concept of analytics aims to understand data. We set out to portray and discuss perspectives of the evolving use of Big Data in science and healthcare and, to examine some of the opportunities and challenges.

Methods: A literature review was conducted to highlight the implications associated with the use of Big Data in scientific research and healthcare innovations, both on a large and small scale.

Results: Scientists and health-care providers may learn …

Efficacy Of Gras Antimicrobial Compounds And Modified Atmosphere Packaging In Reducing Salmonella, Campylobacter And Spoilage Organisms On Poultry Meat, Divek V T Nair

Theses and Dissertations

The antimicrobial efficacy of carvacrol, trans-cinnamaldehyde, eugenol and thyme oil (0.5, 1, 2 and 5% v/v) was evaluated against Salmonella on turkey breast cutlets as 2 min dip treatments at 4°C. Carvacrol revealed maximum efficacy against Salmonella on turkey cutlets and was selected for 30s and 60s dip treatments at 4°C. Only 5% carvacrol was effective and exhibited 1.0 and 1.8 log CFU/g reduction of Salmonella with 30s and 60s dip treatments, respectively. However, carvacrol showed synergistic activity with carbon dioxide in modified atmosphere packaging (95% CO2/5% O2) against Salmonella and Campylobacter and caused 1.0-2.0 log CFU/g reductions with lower …

A Longitudinal Analysis Of Body Mass And Morbidity: Evidence From The Health And Retirement Study, Jamie Boydstun

Theses and Dissertations

This dissertation aimed to investigate the direct and indirect pathways between body mass and three indicators of morbidity – health conditions, disability, and self-rated health status – and how these associations differed across birth cohorts. Using data from the Health and Retirement Study, a nationally representative longitudinal sample of adults age 51 or older, I used latent growth curve modeling to investigate the association of body mass on morbidity for five cohorts. Latent growth curve models allow for changes in body mass, health conditions, disability, and self-rated health over several waves of data to be assessed. To determine if the …

Missed Clinic Visits Predictor Of Mortality In Hiv, Staff

Browse All News

No abstract provided.

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• HIV Conference to Bring New Strategies to Rural Health Care

Calibration Of Neon's Airborne Imaging Spectrometers, Nathan Leisso, Thomas Kampe, Bryan Karpowicz

The Characterization and Radiometric Calibration for Remote Sensing Annual Meeting (CALCON)

The National Ecological Observatory Network (NEON) is a continental-scale ecological observation facility currently under construction by the National Science Foundation (NSF). NEON’s mission is to enable understanding and forecasting of the impacts of land-use change and invasive species by providing the infrastructure and consistent methodologies for the collection of continental-scale ecological data. The Airborne Observation Platform (AOP) will play a unique role in scaling individual in-situ measurements collected by NEON to those collected by external satellite-based remote sensing systems. The airborne payload consists of the NEON Imaging Spectrometer, a waveform LIDAR, and a high-resolution digital camera integrated into a Twin …

Abq Free Press, August 13, 2014, Abq Free Press

ABQ Free Press

No abstract provided.

Coexistence Of Multi-Allelic Polymorphism With Migration And Selection, Andrew Flick

Journal of Undergraduate Research at Minnesota State University, Mankato

Population ecology is concerned with the growth patterns of populations. This field has many applications, ranging from survival at the environmental level, to the spread of infectious diseases at the cellular level. Mathematical modeling and computer simulation can be powerful tools in researching this area. I will be investigating the spatial patterns in populations (or gene frequencies) due to migration and selection. My research conditions are for the maintenance of polymorphism under a variety of migration schemes in discrete-space and continuous-time mathematical models. The results will be applicable from the ecological level to the molecular level. Some species are better …

Materials And Methods For Prevention And Treatment Of Rna Viral Diseases, Shyam S. Mohapatra, Aruna K. Behera

USF Patents

The subject invention concerns a method of inhibiting an RNA virus infection within a patient by increasing the amount of 2-5 oligoadenylate synthetase (2-5 AS) activity within the patient. Preferably, the preventative and therapeutic methods of the present invention involve administering a nucleotide encoding 2-5 AS, or at least one catalytically active fragment thereof, such as the p40, p69, p100 subunits, to a patient in need thereof. The present inventors have determined that overexpression of 2-5AS causes a reduction in epithelial cell damage, reduction in infiltration of mononuclear cells in the peribronchiolar and perivascular regions, and reduction in thickening of …

Trophic Cascade Effects Of Deer Overabundance On Connecticut's Native Vegetation And Small Mammal Populations, Megan A. Floyd

Master's Theses

No abstract provided.

Co-Infection With Hpv Types From The Same Species Provides Natural Cross-Protection From Progression To Cervical Cancer, Rafal S. Sobota, Doreen Ramogola-Masire, Scott M. Williams, Nicola M. Zetola

Dartmouth Scholarship

The worldwide administration of bivalent and quadrivalent HPV vaccines has resulted in cross-protection against non-vaccine HPV types. Infection with multiple HPV types may offer similar cross-protection in the natural setting. We hypothesized that infections with two or more HPV types from the same species, and independently, infections with two or more HPV types from different species, associate with protection from high-grade lesions.

Identifikasi Populasi Bakteri Dalam Spons Pencuci Piring Dengan Metode Pcr-Rflp, Shabarni Gaffar, Iman Permana Maksum, Euis Julaeha

Chimica et Natura Acta

Spons pencuci piring 200.000 kali lebih kotor dibanding dudukan toilet. Berbagai bakteri penyebab penyakit seperti Eschericia coli, Pseudomonas dan Staphylococcus, berkembang biak di permukaan yang basah. Selain itu, 500 ribu bakteri hidup di dalam saluran pembuangan bak cuci piring. Jika spons dalam kondisi tidak kering (lembab karena direndam), maka akan menjadi markas semua bakteri. Penelitian ini bertujuan untuk menentukan populasi bakteri yang terdapat pada spons cuci piring yang basah. Identifikasi dilakukan dengan metoda molekular berbasis DNA yaitu teknik PCR-RFLP gen 16s rDNA. Metoda PCR-RFLP merupakan genetik fingerprinting yang akurat, cepat dan sederhana. Koloni bakteri yang ditumbuhkan dari spons pencuci piring …

What's Happening: August 11, 2014, Maine Medical Center

What's Happening

No abstract provided.

Epoxide-Mediated Differential Packaging Of Cif And Other Virulence Factors Into Outer Membrane Vesicles, A. E. Ballok, L. M. Filkins, J.. M. Bomberger, B. A. Stanton, George A. O'Toole

Dartmouth Scholarship

Pseudomonas aeruginosa produces outer membrane vesicles (OMVs) that contain a number of secreted bacterial proteins, including phospholipases, alkaline phosphatase, and the CFTR inhibitory factor (Cif). Previously, Cif, an epoxide hydrolase, was shown to be regulated at the transcriptional level by epoxides, which serve as ligands of the repressor, CifR. Here, we tested whether epoxides have an effect on Cif levels in OMVs. We showed that growth of P. aeruginosa in the presence of specific epoxi

Commencement Convocation Program, Usf, August 9, 2014, University Of South Florida

USF Graduation and Convocation Programs

King O'Neal Scholars - Robert Ackerman, Sarah Bowermaster, Dariya Dolgolova, and Jennifer Le; President's Fellow Medallion - John Ramil; President's Global Leadership Award - Dr. Jorge Motta; Outstanding Graduate - Denis Marton Menendez; Thomas R. Pickering Graduate Fellow - Denise Marton; James Madison Fellowship - Elizabeth Rasmussen; Benjamin A. Gilman International Scholarship - Adely Castaneda and Najja Pemberton

The Development, Implementation, And Testing Of An Interactive Sexual Health Web-Based Application Intervention To Reduce Sexual Risk Behaviors Among College Students, Dawnyéa Dominique Jackson

Theses and Dissertations

College life is often regarded as a time for exploration and growth. One major area of exploration and growth for college students is sex and sexuality. Growth and exploration involves not only learning how to master these life situations, but also experiencing some negative consequences. Negative consequences for college students engaging in sexual risk-taking behaviors often lead to a high prevalence of sexually transmitted infections (STIs), unintended pregnancies, emotional distress, and large healthcare costs. Given the high prevalence of risky sexual behavior among young adults and the highly negative consequences of risky sexual behavior for this population, it is critical …

Using Comparative Metagenomics To Determine The Role Of Atlantic Bottlenose Dolphins (Tursiops Truncatus) As Sentinels For Human Respiratory Health, Sarah H. Stellick Seepaulsingh

Theses and Dissertations

Cetaceans are proposed as sentinel species for assessing the health of marine ecosystems and human coastal populations. This relationship has been based on similarities in the bioaccumulation of pollutants by cetaceans and humans, as well as their susceptibility to infectious disease. Respiratory disease represents a major cause of mortality in humans and cetaceans, and culture-dependent studies and 16S rDNA gene sequencing have identified known human respiratory pathogens from immunocompromised and healthy dolphins.

To broaden our understanding of the microbial community associated with dolphin upper respiratory tracts (URT), we characterized metagenomes generated from the URT of Atlantic bottlenose dolphins (Tursiops truncatus) …

Analysis Of Risk Factors Contributing To Home–Based Direct Care Workers (Dcws) Occupational Injury In Long–Term Care, Hanadi Y. Hamadi

Theses and Dissertations

Home–based direct care workers (DCWs) provide care in a unique workplace environment: the patient’s home. The high rate of injuries experienced by this subcategory of healthcare workers compared to other industries and occupations make the need to understand the risk factors for these injuries vital. This study builds on prior research and specifically profiles occupational injury patterns among home–based DCWs who deliver care primarily in patient homes and the association of individual, perceived environmental and ergonomic characteristics on predicting occupational injury. The study used a cross–sectional analysis of secondary data from the 2007 National Home Health Aide Survey (NHHAS), sampling …

Women’S Hiv Prevention Study (Whips): A Proposal To Pilot Test An Hiv Intervention For Older African American Women Living With Hiv, Charsey Cherry

Theses and Dissertations

Despite improvements in treatments over the past 30 years, HIV /AIDS continues to be a major public health threat, particularly among sub-populations such as African American women. Midlife and older adults (those aged 40 years and over) are fast becoming a growing concern for HIV/AIDS infections, particularly older African American women. There has been limited research targeting midlife and older African American women living with HIV that aimed to reduce their transmission of HIV and promote them becoming prevention advocates. In response to this gap in the literature, a culturally tailored intervention guided by the IMB Model of behavior change …

Decreasing The Incidence Of Tuberculosis In Renal Transplant Patients: A Single Center Review, Heidi Dauter Msn, Fnp-Bc, Cctc, Brandi Kolokas Ccma, Lynsey S. Biondi Md, Facs

Department of Surgery

No abstract provided.

Increasing Patients’ Knowledge In Transmission Of Bacterial Vaginosis And Its Treatment Regimens, Debra Garner

Dissertations

Research has shown bacterial vaginosis to be one of the most common vaginal disorders in women of child bearing age (Chen, Tian, & Beigi, 2009; Centers for Disease Control, 2012; Menard, 2011). Each year many women are seen by their gynecologist, sexually transmitted infection (STI) clinics, and emergency rooms (ER) for complaints of having malodorous discharge (Bradshaw, et al., 2005). Several STI’s and adverse pregnancy outcomes have been attributed to women having bacterial vaginosis (Bradshaw, et al, 2005; Chen, et al.2009; Elkins, & Mayeaux, 2010). Women are predisposed to bacterial vaginosis by the use of wearing non cotton underwear, douching, …

Fieldwork Summary Report-World Health Organization, Anna Cross

Master's Projects and Capstones

My fieldwork experience took place at the World Health Organization in Geneva, Switzerland, where I completed over 420 hours of practical experience. My fieldwork focused in the field of mental health, working on disorders management (psychosis, depression and suicide) and evidence-based research, but more specifically focusing on schizophrenia, premature death in severe mental disorders, and maternal mental health. Although my fieldwork focused within these areas, for the purpose of this final report, I have opted to focus on my contributions to the research around schizophrenia and premature death within severe mental disorders. Specifically, I contributed to multiple fact/information sheets for …

Safety And Immunogenicity Of Human Serum Albumin-Free Mmr Vaccine In Us Children Aged 12–15 Months, Maurice A. Mufson, Clemente Diaz, Michael Leonardi, Christopher J. Harrison, Stanley Grogg, Antonio Carbayo, Simon Carlo-Torres, Robert Jean Freau, Ana Quintero-Del-Rio, Gisele Bautista, Michael Povey, Christopher Da Costa, Ouzama Nicholson, Bruce L. Innis

Internal Medicine

Background: M-M-RTMII (MMRII; Merck & Co) is currently the only measles-mumps-rubella (MMR) vaccine licensed in the United States. Another licensed vaccine would reinforce MMR supply. This study assessed the immunogenicity of a candidate vaccine (PriorixTM, GlaxoSmithKline Vaccines [MMR-RIT]) when used as a first dose among eligible children in the United States.

Methods: In this exploratory Phase-2, multicenter, observer-blind study, 1220 healthy subjects aged 12–15 months were randomized (3:3:3:3) and received 1 dose of 1 of 3 MMR-RIT lots with differing mumps virus titers (MMR-RIT-1 [4.8 log10]; MMR-RIT-2 [4.1 log10]; MMR-RIT-3 [3.7 log10] CCID50) or MMRII co-administered with hepatitis Avaccine (HAV), …

10 Essential Facts About Ebola, Staff

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No abstract provided.

Phenotypic Signatures Arising From Unbalanced Bacterial Growth, Cheemang Tan, Robert P. Smith, Ming-Chi Tsai, Russell Schwartz, Lingchong You

Biology Faculty Articles

Fluctuations in the growth rate of a bacterial culture during unbalanced growth are generally considered undesirable in quantitative studies of bacterial physiology. Under well-controlled experimental conditions, however, these fluctuations are not random but instead reflect the interplay between intra-cellular networks underlying bacterial growth and the growth environment. Therefore, these fluctuations could be considered quantitative phenotypes of the bacteria under a specific growth condition. Here, we present a method to identify “phenotypic signatures” by time-frequency analysis of unbalanced growth curves measured with high temporal resolution. The signatures are then applied to differentiate amongst different bacterial strains or the same strain under …