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Development And Implementation Of Assessment Methods For Tissue-Engineered Blood Vessel Mimics, Tanner Stevenson

Biomedical Engineering

Coronary Artery Disease (CAD), the most prevalent form of heart disease, is the result of clogged or damaged coronary arteries and claims around 380,000 Americans annually. A common treatment for CAD involves placing a stent into the artery in order to open the lumen and support the native tissue—a procedure that drastically reduces patient recovery times in comparison to heart bypass surgery. However, stents do not always interact well with the body and require additions such as surface coatings or drug elution in order for additional biocompatibility. These additions necessitate extensive in vitro and in vivo testing which are expensive …

A Multi-Compartment Model Of The Normal Menstrual Cycle: Integrating Hormonal, Ovarian, And Endometrial Elements, Victoria Lea Wolf

Theses and Dissertations

The uterine endometrium undergoes cyclical phases of cell proliferation, cell differentiation, and menstruation under the influence of the ovarian hormones, estrogen and progesterone. Since the data necessary to create a classical kinetic model of these signaling pathways is lacking, we used a Boolean network approach that includes the influences of various growth factors and the differential expression of their receptors under the influence of estrogen and progesterone. Results show a gain in endometrial tissue and loss of tissue during menstruation that mirrors what can be expected over the course of a normal menstrual cycle in women, where the endometrium typically …

Innovative Tertiary Prevention For Women Who Seek Shelter Services Greenhouse 17, Diane Fleet, Diane R. Follingstad, Claire M. Renzetti

NSF-NIJ IPV Prevention Workshop

No abstract provided.

Towards Structural Systems Pharmacology To Study Complex Diseases And Personalized Medicine, Lei Xie, Xiaoxia Ge, Hepan Tan, Li Xie, Yinliang Zhang, Thomas Hart, Xiaowei Yang, Philip E. Bourne

Publications and Research

Genome-Wide Association Studies (GWAS), whole genome sequencing, and high-throughput omics techniques have generated vast amounts of genotypic and molecular phenotypic data. However, these data have not yet been fully explored to improve the effectiveness and efficiency of drug discovery, which continues along a one-drug-one-target-one-disease paradigm. As a partial consequence, both the cost to launch a new drug and the attrition rate are increasing. Systems pharmacology and pharmacogenomics are emerging to exploit the available data and potentially reverse this trend, but, as we argue here, more is needed. To understand the impact of genetic, epigenetic, and environmental factors on drug action, …

Identification Of Microrna Biomarkers In Her2-Positive Breast Cancer, Hossam Tashkandi

Theses and Dissertations

Human epidermal growth factor receptor 2 (HER2) is overexpressed in approximately 30% of breast cancer patients with poor prognosis. In addition, microRNAs are small non-coding RNA that have been linked to many cancers. Here we investigate which miRNAs are differentially regulated by HER2 overexpression. Using quantitative reverse-transcription prolymerase chain reaction (QRT-PCR) and matching it with the clinical data acquired from Dvinge, we find five candidate miRNAs. When comparing the miRNAs’ effect on patient survival, only three miRNAs stand as good predictors of patient survival outcome. These miRNAs are miR-146a-5p, miR-181d, and miR-195-5p. When miR-146a-5p is up-regulated, which is the case …

D-Aspartic Acid As A Nutritional Aid To Increase Serum Testosterone, Logan Joseph Rodgers

Electronic Theses and Dissertations Archive

The purpose of this proposed study was to determine the potential changes in testosterone (free and total) and estradiol over a four-week intervention period using D-aspartic acid (DAA) in men. Twenty-four physically active men, aged 18-39 years, were randomly assigned to two groups (conditions) using a placebo-controlled, double-blind design. Baseline values for all hormones were compared to values measured at the end of two and four weeks of DAA administration, provided at a dosage of 3.12g, compared to the placebo. Data were analyzed using a 2 (condition) x 3 (time) analysis of variance. No interaction or main effects were noted …

Effect Of Whole Beetroot On Muscular Endurance And Power In Powerlifters And Physique Competitors, John H. Jewett

Theses & Dissertations

Nitrate ingestion improves aerobic exercise performance, but the efficacy in resistance training has not been established. The purpose of this study was to investigate whether consuming whole beetroot, as a means to ingest nitrate, had an ergogenic benefit in muscular power and endurance in resistance training in powerlifters and physique competitors. Thirteen men and women (mean ± SE age, 21.2 ± 0.8 y; mass, 83.1 ± 5.9 kg) that had competed in a powerlifting or a physique competition in the previous year were studied in a randomized double blind controlled crossover trial with a seven day washout period. Participants ingested …

Consequences Of Endogenous Mitochondrial Oxidative Stress In The Heart, Kristin Alisha Koenig

Dissertations, Masters Theses, Capstones, and Culminating Projects

For many years, it has been proposed that oxidative stress within the mitochondria causes mutations to the mitochondrial genome, resulting in changes in copy number per single cell. Ultimately, this compensatory increase in mtDNA, coupled with an increase in mutations, has been suggested to play a major role in the pathobiology of aging. However, evidence for this phenomenon is somewhat controversial. Importantly, oxidative stress can vary between individual cells; therefore, the overall goal of this project is to determine if oxidative stress causes both an altered copy number for mtDNA in singlecardiomyocytes and develop a methodology for sequencing mtDNA from …

Detection Methods For Small Analytes: Improvement Of Novel Spr Technology Detection Of Toxins And Toxicants, William B. Ollayos

Honors Scholar Theses

Humans are exposed to a variety of toxic substances in their day-to-day activities that may have adverse health effects if not properly identified. An integrated dual-mode instrument that combines grating-coupled surface plasmon resonance (GCSPR) and grating-coupled surface plasmon coupled emissions (GCSPCE) should allow for the efficient detection of toxic analytes in a high-content system using gold-coated superparamagnetic nanobeads. The goal of this study is to validate the binding characteristics of the reagents intrinsic to this innovative assay methodology. Competition enzyme-linked immunosorbent assays (ELISA) and surface plasmon resonance (SPR) assays were conducted on NNK toxicants and SEB toxins, which were the …

Stability Of Nano-Encapsulated Rice Bran Derived Bioactive Pentapeptide In Apple Juice, Fatima Mohammed Alessa

Graduate Theses and Dissertations

Cereal grains and their components derived Bioactive compounds such as rice bran can promote health and can be derived from Rice bran contains 12-20 % protein and could be a good source for extracting bioactive peptides. A pentapeptide with a secqunce of amino acids Glu-Gln-Arg-Pro-Arg (EQRPR) has been prepared from heat stablized defatted rice bran (HDRB) and has demonstrated anti-cancer proprerties in-vitro. This bioactive pentapeptide can thus be used as a nutraceutical by incorporating it into a suitable food system. Fruit juces can be vehicles to incorporate this pentapeptide. Fruit juices contribute to about 60% of the consumed beverages in …

Exertional Rhabdomyolysis: Studying The Perfect Storm, Nicholas B. Holtgrieve

All-Inclusive List of Electronic Theses and Dissertations

Background: Rhabdomyolysis is a potentially life-threatening syndrome characterized by the breakdown of skeletal muscle fibers resulting in the release of proteins into general circulation. Common practice for diagnosing exertional rhabdomyolysis (ER) is using biochemical markers for muscle damage; creatine kinase (CK) and myoglobin (Mb) are proteins detected in the bloodstream after muscle injury or trauma. Serum creatinine is the best biochemical marker for calculating renal function. Measuring GFR is not common when assessing ER. Purpose: The aim of this study is to help predict ER. Methods: Ninety-three athletes volunteered for the research study. Participants were asked to give blood three …

Reducing "Failure To Rescue" Occurrences: A Pilot Project Incorporating High-Fidelity Simulation During Mock Codes, To Enhance Pediatric Nurses' Clinical Knowledge And Skills, Denise Dawkins

Doctoral Projects

Thanks to advances in technology, the survival rate of infants and children with critical illnesses has improved. One consequence has been an increase in the acuity levels of hospitalized pediatric patients, which may heighten the risk of in-hospital cardiopulmonary arrest. Patient safety can be compromised by nurses who do not detect subtle clinical changes in a patient's condition, do not perform accurate interventions, or do not apply critical thinking (failure to rescue). The Institute of Medicine (10M) in 1999 estimated that approximately 100,000 people died each year as the result of poor care.

Patients and families place great trust in …

Acute Effects Of Long Distance Running On C-Reactive Protein And Arterial Stiffness, Bridget E. Durocher

All NMU Master's Theses

Abstract

Central arterial stiffness, indicated by pulse wave velocity (PWV), is increased during the hour post ultramarathon (ULT), but it's currently unknown if PWV remains elevated 24 hours after ULT or marathon (MAR). PURPOSE: To determine the effects of ULT and MAR on C-reactive protein (CRP), aortic blood pressure (BP), aortic augmentation index (AIx), PWV, and LF:HF ratio. We hypothesized that CRP, AIx, PWV and LF:HF ratio would be elevated 24 hours post-race. Our secondary hypothesis was that changes in PWV would be inversely correlated to the percentage of moderate activity (3-6 METs) during competition. METHODS: Applanation tonometry was used …

Imbalance Between Neutrophil Elastase And Elafin Promotes Breast Cancer Growth And Progression, Joseph Anthony Caruso

Dissertations and Theses (Open Access)

Elafin, an endogenous serine protease inhibitor, is a critical component of the epithelial barrier against neutrophil elastase (NE) activity. The central hypothesis examined in this dissertation was that elafin has tumor suppressive properties in breast cancer. In support of this hypothesis, immunohistochemical (IHC) analysis revealed that elafin was downregulated in the majority of invasive breast tumors and a subset of pre-invasive ductal carcinoma in situ (DCIS) compared to elafin expression in the normal mammary epithelium. To understand the role of elafin in the mammary epithelium and the impetus for its downregulation during breast tumorigenesis, primary and immortalized human mammary epithelial …

Computational Models Of Intracellular And Intercellular Processes In Developmental Biology, Ahmadreza Ghaffarizadeh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Systems biology takes a holistic approach to biological questions as it applies mathematical modeling to link and understand the interaction of components in complex biological systems. Multiscale modeling is the only method that can fully accomplish this aim. Mutliscale models consider processes at different levels that are coupled within the modeling framework. A first requirement in creating such models is a clear understanding of processes that operate at each level. This research focuses on modeling aspects of biological development as a complex process that occurs at many scales. Two of these scales were considered in this work: cellular differentiation, the …

Measuring The Effects Of An American Physiological Society’S Phun Week Activity On 2nd Grade Students, James Edelman

All-Inclusive List of Electronic Theses and Dissertations

In the last few years the United States has been on the decline in the fields of science and technology. The American Physiological Society (APS) has developed a program to engage young minds with scientists. Physiology Understanding Week (PhUn) is the APS initiative to use interactive experiments to engage school-age children with science and physiology. Even though PhUn Week has been going on since 2005, systematic assessment of the activities effects is rare. Here, we sought to assess if an APS PhUn Week activity enhances physiology understanding and interest in science in second grade children form a rural area. The …

N, N-Dimethyltryptamine And Biological Reductive Accounts For Religious And Spiritual Experiences, Shaun Smith

2014-2016 Graduate

There is unquestionably a plethora of details and mysteries regarding the mind and the body. However, with the advent of psychopharmacology (the study of how psychedelics inform or alter brain states), there are more issues at hand. Do psychedelics allow us to access deeper areas of our consciousness? Are we having a spiritual experience under the influence of psychedelics? Dr. Rick Strassman does not want to continue asking these rather conspiratorial-like questions. Instead, Dr. Strassman believes that there is one special, endogenous psychedelic, synthesized within the human physiological framework: N, N-Dymthethyltryptamine. Dr. Strassman concludes that this chemical is produced within …

Measuring And Predicting Total Energy Expenditure Among Highly Active Humans In Natural Environments, Cara Ocobock

All Theses and Dissertations (ETDs)

The current model for predicting human total energy expenditure (TEE), the Factorial Method, significantly underestimates actual TEE, particularly among highly active populations. In this study, the Allocation Model is presented for predicting TEE. Unlike the Factorial Method, the Allocation Model includes metabolic cost terms for both thermoregulation and the thermic effect of food, as well as using more accurate basal metabolic rate and activity cost estimations. The Allocation Model was tested using doubly labeled water and flex-heart rate measured TEEs of healthy, highly active adults (N=56) participating in National Outdoor Leadership School semester long courses. Two of the semester-long courses …

A Novel Post-Electrospinning Treatment To Improve The Fibrous Structure Of The Chitosan Membrane, Hengjie Su

Electronic Theses and Dissertations Archive

Guided bone regeneration (GBR) membranes function as barriers in preventing soft tissue migration into dental osteogenic spaces. Chitosan is a biocompatible and degradable natural polysaccharide, which has shown potential in tissue regeneration and GBR applications. Trifluoroacetic acid (TFA) is a common solvent used to make the chitosan polymer solution for electrospinning process. However, the TFA salts remaining in the electrospun nano-sized fibers induce the chitosan fiber swelling and dissolution when placed in aqueous solutions leading to loss of nano-fiber structure. The aim of this work was to evaluate an innovative method using triethylamine (TEA)/ di-tert-butyl dicarbonate (tBoc) to treat the …

Cerebellar Purkinje Cell Loss In Developing Lurcher Mice Influences Respiratory Compensatory Responses To Acute Hypercapnia And Hypoxia, Michele Ann Calton

Electronic Theses and Dissertations Archive

Impaired responsivity to hypercapnia or hypoxia is commonly considered a mechanism of failure in Sudden Infant Death Syndrome (SIDS). We used a model of developmental cerebellar Purkinje cell loss to determine if such loss influenced compensatory ventilatory responses to hypercapnic and hypoxic challenges. Twenty-four Lurcher mutant mice and wildtype controls were sequentially exposed to 2% increases in CO2 (0%-8%) or 2% reductions in O2 (21%-13%) for four-minutes, with return to room air between each exposure. Whole-body plethysmography was used to continuously monitor tidal volume and breath frequency. Tidal volume increases to CO2 elevation were reduced in Mutants, and slower to …

Walk-Training Increases Expression Of Gdnf In Pectoralis Muscle But Not Diaphragm From Mouse, Erin Donovan

Honors Theses

Neurotrophic factors are proteins that play an important role in the development and maintenance of neurons. Recent studies have shown that neurotrophic factors may hold promise for treating damage to the nervous system caused by trauma or diseases, such as amyotrophic lateral sclerosis or Parkinson’s disease. Glial cell line-derived neurotrophic factor (GDNF) is expressed in skeletal muscles and affects peripheral motor neurons. Results of previous studies have demonstrated that exercise can increase GDNF content in skeletal muscle of rat. The goal of the current study was to examine whether expression of GDNF in skeletal muscle of mouse is also regulated …

The Relationship Between Body Composition And Thermal Responses To Hot And Cold Water Immersion, Jessica M. Stephens, Christos Argus, Matthew W. Driller

Journal of Human Performance in Extreme Environments

The current study aimed to examine the influence of body fat percentage on thermal responses to water immersion at the two temperature extremes used in athletic recovery, by comparing the thermal responses of low- and high-fat individuals to immersion in hot water (HWI) or cold water (CWI). Thirty-nine subjects completed a body composition assessment (to determine low-fat and high-fat groups), and 15 minutes of either CWI (n = 20) or HWI (n = 19), followed by 30 minutes of passive rest. Core temperature (Tc), skin temperature (Tsk), and thermal sensation (TSS) were recorded at various time points during …

Comparative Analysis Of Ancient Ritual Sacrifice, Alexandra Price

UROP Posters

I am studying ritual human and animal sacrifice using Winkleman’s 1998 Standard Cross Cultural Sample method because I want to analyze similarities between cultures with sacrifice in order to help my reader understand why ritual sacrifice developed in civilization’s that had little to no contact with each other.

Professor Lin Aanonsen Wins Thomas Jefferson Award, Macalester College

College Press Releases

No abstract provided.

Optimization And Standardization Of The Oxygen Radical Absorbance Capacity (Orac) Assay, Richard Low, Dr. Tory Parker

Journal of Undergraduate Research

Antioxidants, when consumed, have the ability to neutralize free radicals, suggesting their presence may protect human physiology from destructive oxidative processes1. Several assays have been developed that quantify the antioxidant capacity of compounds, and one assay that is commonly utilized by researchers and industry is the Oxygen Radical Absorbance Capacity (ORAC) assay2. Despite its popularity, significant differences in reported ORAC values occur between laboratories, leading to difficulties in comparing research. Dr. Parker and his lab conducted a series of tests to attempt to standardize and optimize the ORAC assay.

Teacher Internship Report - Anderson Union High School, Kathryn L. Teixeira

Agricultural Education: Graduate Internship Reports

This internship/project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Anderson Union High School conducted by representatives of the California Department of Education. The internship included the practical application of occupational skills related to the horticulture industry and modification to the Greenhouse facilities at Anderson Union High School by improving ergonomics of greenhouse tables, replacing the existing siding of storage facilities and installing concrete walkways within the greenhouse. Students in the Agricultural Department were integral in all parts …

9th Annual Research In The Capitol [Program], April 1, 2014, University Of Northern Iowa. University Honors Program., Iowa State University. Honors Program., University Of Iowa. Honors Program.

Research in the Capitol Programs

Program of research presentations given at the Capitol by students from the University of Northern Iowa, Iowa State University, and the University of Iowa.

Program And Proceedings: Nebraska Academy Of Sciences 1880–2014, 134th Anniversary Year, One Hundred-Twenty-Fourth Annual Meeting, April 11, 2014

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Collegiate Academy: Biology

Chemistry and Physics

Collegiate Academy: Chemistry and Physics

Applied Science and Technology

Biological and Medical Sciences

Aeronautics and Space Science, Poster Session

Anthropology

Maiben Memorial Lecture: "Education, Careers and Opportunities in Engineering and Metallurgy in Nebraska," Terry Rasmussen, Nucor Steel, Norfolk, Nebraska

Teaching of Science and Mathematics

Anthropology

Earth Science

The Roles Of Phosducin-Like Protein 1 And Programmed Cell Death Protein 5 As Molecular Co-Chaperones Of The Cytosolic Chaperonin Complex, Christopher M. Tracy

Theses and Dissertations

A fundamental question in biology is how proteins, which are synthesized by the ribosome as a linear sequence of amino acids, fold into their native functional state. Many proteins require the assistance of molecular chaperones to maneuver through the folding process to protect them from aggregation and to help them reach their native state in the very concentrated protein environment of the cell. This study focuses on the roles of Phosducin-like Protein 1 (PhLP1) and Programmed Cell Death Protein 5 (PDCD5) as molecular co-chaperones of the Cytosolic Chaperonin Complex (CCT).Signaling in retinal photoreceptors is mediated by canonical G protein pathways. …

A Rat Model Of Mild Blast-Induced Head Trauma With Structural And Biochemical Abnormalities In The Absence Of Motor Deficits, Michael Kelso Walls

Open Access Theses

Blast injury has been coined the "signature injury of the Iraq war" resulting in a surge of interest in understanding and addressing the corresponding pathological sequelae. As modern warfare develops into a distinctly guerrilla style combat by smaller terrorist groups against larger, regimented forces, tactics like suicide bombing and roadside bombing with Improvised Explosive Devices (IEDs) become more prevalent. 1,2 This increased use of IEDs countered with improved body armor and protective technologies has significantly increased the survival rate of such attacks. 1.2 However, as survival rates have improved with body armor and other protective technologies, the incidence of reported …