Digital Commons Network^™

Phosphatase-Inert Glucosamine 6‑Phosphate Mimics Serve As Actuators Of The Glms Riboswitch, Xiang Fei, Thomas Holmes, Julianna Diddle, Lauren Hintz, Dan Delaney, Alex Stock, Danielle Renner, Molly Mcdevitt, David B. Berkowitz, Juliane K. Soukup

David Berkowitz Publications

The glmS riboswitch is unique among generegulating riboswitches and catalytic RNAs. This is because its own metabolite, glucosamine-6-phosphate (GlcN6P), binds to the riboswitch and catalytically participates in the RNA selfcleavage reaction, thereby providing a novel negative feedback mechanism. Given that a number of pathogens harbor the glmS riboswitch, artificial actuators of this potential RNA target are of great interest. Structural/kinetic studies point to the 2- amino and 6-phosphate ester functionalities in GlcN6P as being crucial for this actuation. As a first step toward developing artificial actuators, we have synthesized a series of nine GlcN6P analogs bearing phosphatase-inert surrogates in place …

Highly Selective Gas Sensor Arrays Based On Thermally Reduced Graphene Oxide, Alexey Lipatov, Alexey Varezhnikov, Peter Wilson, Victor Sysoev, Andrei Kolmakov, Alexander Sinitskii

Department of Chemistry: Faculty Publications

The electrical properties of reduced graphene oxide (rGO) have been previously shown to be very sensitive to surface adsorbates, thus making rGO a very promising platform for highly sensitive gas sensors. However, poor selectivity of rGO-based gas sensors remains a major problem for their practical use. In this paper, we address the selectivity problem by employing an array of rGO-based integrated sensors instead of focusing on the performance of a single sensing element. Each rGO-based device in such an array has a unique sensor response due to the irregular structure of rGO films at different levels of organization, ranging from …

Analogues Of (-)-Picropodophyllin, Synthesis And Uses Thereof, David B. Berkowitz, Sylvain Broussy

Department of Chemistry: Faculty Publications

Analogs of (-)-Picropodophyllin, synthesis thereof, and uses in pharmaceuticals as inhibitors of IGF1RK.

Β-Keto And Β-Hydroxyphosphonate Analogs Of Biotin-5’-Amp Are Inhibitors Of Holocarboxylase Synthetase, Wantanee Sittiwong, Elizabeth Cordonier, Janos Zempleni, Patrick Dussault

Patrick Dussault Publications

Holocarboxylase synthetase (HLCS) catalyzes the covalent attachment of biotin to cytoplasmic and mitochondrial carboxylases, nuclear histones, and over a hundred human proteins. Nonhydrolyzable ketophosphonate (β-ketoP) and hydroxyphosphonate (β-hydroxyP) analogs of biotin-5′-AMP inhibit holocarboxylase synthetase (HLCS) with IC₅₀ values of 39.7 μM and 203.7 μM. By comparison, an IC₅₀ value of 7 μM was observed with the previously reported biotinol-5'-AMP. The K_i values, 3.4 μM and 17.3 μM, respectively, are consistent with the IC50 results, and close to the K_i obtained for biotinol-5'-AMP (7 μM). The β-ketoP and β-hydroxyP molecules are competitive inhibitors of HLCS while biotinol-5'-AMP …

Oxacycle Synthesis Via Intramolecular Reaction Of Carbanions And Peroxides, Rachel Willand-Charnley, Benjamin W. Puffer, Patrick Dussault

Patrick Dussault Publications

The intramolecular reaction of dialkyl peroxides with carbanions, generated via chemoselective metal-heteroatom exchange or deprotonation, provides a new approach to cyclic ethers. Applied in tandem with C–C bond formation, the strategy enables a one-step annelation to form oxaospirocycles.

Urban Ecology: Advancing Science And Society, Colby J. Tanner, Frederick R. Adler, Nancy B. Grimm, Peter M. Groffman, Simon A. Levin, Jason Munshi-South, Diane E. Pataki, Mitchell Pavao-Zuckerman, William G. Wilson

School of Biological Sciences: Faculty Publications

Urban ecology has quickly become established as a central part of ecological thinking. As cities continue to grow in size and number, two questions serve to unify this broad and multidisciplinary research landscape: (1) how can urban ecology contribute to the science of ecology, and (2) how can urban ecology be applied to make cities more livable and sustainable? In spite of the advances made thus far, there are many unexplored ways of integrating the science and application of urban ecology. Although scientists assess and make predictions regarding the connections between environmental and socioeconomic processes, practitioners involved in real-world application …

Integrating Evolutionary And Functional Tests Of Adaptive Hypotheses: A Case Study Of Altitudinal Differentiation In Hemoglobin Function In An Andean Sparrow, Zonotrichia Capensis, Zachary A. Cheviron, Chandrasekhar Natarajan, Joana Projecto-Garcia, Douglas K. Eddy, Jennifer Jones, Matthew D. Carling, Christopher C. Witt, Hideaki Moriyama, Roy E. Weber, Angela Fago, Jay F. Storz

School of Biological Sciences: Faculty Publications

In air-breathing vertebrates, the physiologically optimal blood-O_² affinity is jointly determined by the prevailing partial pressure of atmospheric O₂, the efficacy of pulmonary O₂ transfer, and internal metabolic demands. Consequently, genetic variation in the oxygenation properties of hemoglobin (Hb) may be subject to spatially varying selection in species with broad elevational distributions. Here we report the results of a combined functional and evolutionary analysis of Hb polymorphism in the rufouscollared sparrow (Zonotrichia capensis), a species that is continuously distributed across a steep elevational gradient on the Pacific slope of the Peruvian Andes. We integrated …

The Pseudomonas Syringae Type Iii Effector Hopd1 Suppresses Effector-Triggered Immunity, Localizes To The Endoplasmic Reticulum, And Targets The Arabidopsis Transcription Factor Ntl9, Anna Block, Tania Y. Toruno, Christian G. Elowsky, Chi Zhang, Jens Steinbrenner, Jim Beynon, James R. Alfano

Nebraska Center for Biotechnology: Faculty and Staff Publications

Pseudomonas syringae type III effectors are known to suppress plant immunity to promote bacterial virulence. However, the activities and targets of these effectors are not well understood. We used genetic, molecular, and cell biology methods to characterize the activities, localization, and target of the HopD1 type III effector in Arabidopsis. HopD1 contributes to P. syringae virulence in Arabidopsis and reduces effector-triggered immunity (ETI) responses but not pathogen-associated molecular pattern-triggered immunity (PTI) responses. Plants expressing HopD1 supported increased growth of ETI-inducing P. syringae strains compared with wild-type Arabidopsis. We show that HopD1 interacts with the membrane-tethered Arabidopsis transcription factor NTL9 and …

The Role Of Mindfulness And Psychological Capital On The Well-Being Of Leaders, Maree Roche, Jarrod M. Haar, Fred Luthans

Department of Management: Faculty Publications

In today’s highly competitive and extremely complex global economy, organizational leaders at all levels are facing unprecedented challenges. Yet, some seem to be handling the pressure better than others. Utilizing 4 samples of CEOs/presidents/top (n = 205), middle (n = 183), and junior (n = 202) managers, as well as 107 entrepreneurs, using Structural Equation Modeling we tested the direct effect that their level of mindfulness (heightened awareness) and the mediating effect of their psych logical capital (i.e., hope, efficacy, resilience, and optimism) may have on their mental well-being. In all 4 samples, mindfulness was found to be negatively related …

Interprofessional Curriculum On Environmental And Social Determinants Of Health In Rural Kenya: Aga Khan University East Africa University Of California San Francisco Integrated Primary Health Care Program, M. R. Mahoney, K. Baltzell, Esther Nderitu, R. Dhanani, S. Macfarlane

School of Nursing & Midwifery, East Africa

The Aga Khan University East Africa (AKU)-University of California San Francisco (UCSF) Integrated Primary Health Care Program (IPHC) is a public–private partnership with community and government to strengthen the primary health care (PHC) system in Kenya. IPHC provides opportunities for health professions students to work and learn together in the rural and underserved district of Kaloleni.

Tree-Ring Stable Isotopes Record The Impact Of A Foliar Fungal Pathogen On Co2 Assimilation And Growth In Douglas-Fir, Brandy J. Saffell, Frederick C. Meinzer, Steven L. Voelker, David C. Shaw, J. Renée Brooks, Barbara Lachenbruch, Jennifer Mckay

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Swiss needle cast (SNC) is a fungal disease of Douglas-fir (Pseudotsuga menziesii) that has recently become prevalent in coastal areas of the Pacific Northwest. We used growth measurements and stable isotopes of carbon and oxygen in tree-rings of Douglas-fir and a non-susceptible reference species (western hemlock, Tsuga heterophylla) to evaluate their use as proxies for variation in past SNC infection, particularly in relation to potential explanatory climate factors. We sampled trees from an Oregon site where a fungicide trial took place from 1996 to 2000, which enabled the comparison of stable isotope values between trees with and …

Faktor Yang Mempengaruhi Kesejahteraan Psikologi Anggota Tentera Darat Malaysia, Wan Norhayati Wan Othman

Student Works (2010-2019)

Dengan melihatkan keadaan semasa anggota Tentera Darat Malaysia di mana banyak masalah yang berlaku, ramai yang berhenti awal dan niat untuk berhenti awal, jadi tujuan kajian ini secara umumnya ialah untuk melihat keadaan kesejahteraan psikologi anggota Tentera Darat Malaysia yang masih berkhidmat. Manakala secara khususnya tujuan kajian ini dilakukan adalah untuk mengukur tahap kesejahteraan psikologi anggota Tentera Darat Malaysia, mengenalpasti profil kesejahteraan psikologi anggota Tentera Darat Malaysia berdasarkan enam dimensi mengikut teori Ryff, melihat perbezaan min indeks kesejahteraan psikologi anggota Tentera Darat Malaysia berdasarkan faktor demografi, melihat hubungan pembolehubah bebas iaitu penghayatan agama, kecerdasan emosi, penghargaan kendiri, kepuasan kerja dan …

Rural Latino Adolescent Health: Preliminary Examination Of Health Risks And Cultural Correlates, Timothy D. Nelson, Katherine M. Kidwell, Brian E. Armenta, Lisa J. Crockett, Gustavo Carlo, Les B. Whitbeck

Department of Psychology: Faculty Publications

Latino adolescents living in rural settings may be at increased risk of health problems; however, data describing the health status of this population are limited. This study examined 60 rural Latino adolescents and found high rates of health risk, including at-risk/clinical results for hemoglobin A1C (23.3%), high-density lipoprotein cholesterol (55%), systolic blood pressure (21.7%), and overweight/obesity (55%). Time in sedentary behaviors was high and physical activity was limited. Adolescent language use was associated with health risk status, with greater use of English associated with lower risk. Health psychologists could promote improved health by providing health behavior interventions to this underserved …

Contribution Of Reactive And Proactive Control To Children’S Working Memory Performance: Insight From Item Recall Durations In Response Sequence Planning, Nicolas Chevalier, Tiffany D. James, Sandra A. Wiebe, Jennifer Mize Nelson, Kimberly Espy

Department of Psychology: Faculty Publications

The present study addressed whether developmental improvement in working memory span task performance relies upon a growing ability to proactively plan response sequences during childhood. Two hundred thirteen children completed a working memory span task in which they used a touchscreen to reproduce orally presented sequences of animal names. Children were assessed longitudinally at 7 time points between 3 and 10 years of age. Twenty-one young adults also completed the same task. Proactive response sequence planning was assessed by comparing recall durations for the 1st item (preparatory interval) and subsequent items. At preschool age, the preparatory interval was generally shorter …

The Longitudinal Impact Of Intimate Partner Aggression And Relationship Status On Women’S Physical Health And Depression Symptoms, Laura E. Watkins, Anna E. Jaffe, Lesa Hoffman, Kim L. Gratz, Terri L. Messman-Moore, David Dilillo

Department of Psychology: Faculty Publications

Intimate partner aggression (IPA) has many detrimental effects, particularly among young women. The present study examined the longitudinal effects of IPA victimization and relationship status on physical health and depression symptoms in a sample of 375 community women between the ages of 18 and 25 years. All variables were assessed at 4 occasions over a 12-month period (i.e., 1 assess-ment every 4 months). Multilevel modeling revealed that IPA victimization had both between- and within-person effects on women’s health outcomes, and relationship status had within-person effects when women did not report current IPA. Although IPA was generally related to greater physical …

Effect Of Head Impacts On Diffusivity Measures In A Cohort Of Collegiate Contact Sport Athletes, Thomas W. Mcallister, James C. Ford, Laura A. Flashman, Arthur C. Maerlender, Richard M. Greenwald, Jonathan G. Beckwith, Richard P. Bolander, Tor D. Tosteson, John H. Turco, Rema Raman, Sonia Jain

Department of Psychology: Faculty Publications

Objective: To determine whether exposure to repetitive head impacts over a single season affects white matter diffusion measures in collegiate contact sport athletes.

Methods: A prospective cohort study at a Division I NCAA athletic program of 80 nonconcussed varsity football and ice hockey players who wore instrumented helmets that recorded the acceleration time history of the head following impact, and 79 non–contact sport athletes. Assessment occurred preseason and shortly after the season with diffusion tensor imaging and neurocognitive measures.

Results: There was a significant (p 5 0.011) athlete-group difference for mean diffusivity (MD) in the corpus callosum. Postseason fractional anisotropy …

3d Dynamic Visualization Of Swallowing From Multi-Slice Computed Tomography, Andrew K. Ho, Mark A. Nicosia, Angela Dietsch, William Pearson, Jana Rieger, Nancy Pearl Solomon, Maureen Stone, Yoko Inamoto, Eiichi Saitoh, Sheldon Green, Sidney Fels

Department of Special Education and Communication Disorders: Faculty Publications

Human swallowing and its disorders (dysphagia) are still poorly understood, and yet many speech-language pathologists (SLPs) need to be trained to recognize correct, incorrect, and potentially dangerous swallows. The anatomy of the head and neck region is notoriously complex and difficult to visualize and study. Currently, training programs that teach SLPs to recognize swallowing disorders use artistically derived animations of swallowing, rendered at fixed viewpoints, to help students visualize the anatomy of the head and neck region.

This work improves on these animations by using state-of-the-art medical images to create a dynamic, interactive, 3D simulation of human swallowing. Images of …

The Effect Of Barium On Perceptions Of Taste Intensity And Palatability, Angela M. Dietsch, Nancy Pearl Solomon, Catriona M. Steele, Cathy A. Pelletier

Department of Special Education and Communication Disorders: Faculty Publications

Barium may affect the perception of taste intensity and palatability. Such differences are important considerations in the selection of dysphagia assessment strategies and interpretation of results. Eighty healthy women grouped by age (younger, older) and genetic taste status (supertaster, nontaster) rated intensity and palatability for seven tastants prepared in deionized water with and without 40 % w/v barium: noncarbonated and carbonated water, diluted ethanol, and high concentrations of citric acid (sour), sodium chloride (salty), caffeine (bitter), and sucrose (sweet). Mixed-model analyses explored the effects of barium, taster status, and age on perceived taste intensity and acceptability of stimuli. Barium was …

Characterization Of Sex-Based Differences In The Mechanical Properties Of Human Finger Glabrous Tissue Using A Fiberoptic Sensor, Lalit Venkatesan, Steven M. Barlow

Department of Special Education and Communication Disorders: Faculty Publications

TAC-Cell is a custom-built somatosensory stimulator that delivers pneumatic cutaneous tactile inputs to virtually any skin target on the body and by virtue of its non-ferrous materials is compatible with functional magnetic resonance imaging (fMRI) and magnetoencephalography (MEG) brain scanners. In this study, we describe the method to measure apparent skin displacement induced by TAC-Cell stimulation of the glabrous surface of the distal phalanx of the index finger. Specifically, we studied the effect of four servo controller input voltages (0.4V to 1.0V) on resultant skin displacement among eighteen, neurotypical adult male and female participants. A fiberoptic displacement sensor, commonly used …

Amplitude-Integrated Eeg And Range-Eeg Modulation Associated With Pneumatic Orocutaneous Stimulation In Preterm Infants, Steven M. Barlow, Priya Jegatheesan, Sunshine Weiss, Balaji Govindaswami, Jaehoon Lee, Austin Oder, Dongli Song

Department of Special Education and Communication Disorders: Faculty Publications

Background—Controlled somatosensory stimulation strategies have demonstrated merit in developing oral feeding skills in premature infants who lack a functional suck, however, the effects of orosensory entrainment stimulation on electrocortical dynamics is unknown.

Objective—To determine the effects of servo-controlled pneumatic orocutaneous stimulation presented during gavage feedings on the modulation of aEEG and rEEG activity.

Methods—Two-channel EEG recordings were collected during 180 sessions that included orocutaneous stimulation and non-stimulation epochs among 22 preterm infants (mean gestational age = 28.56 weeks) who were randomized to treatment and control ‘sham’ conditions. The study was initiated at around 32 weeks post-menstrual age (PMA). The raw …

Modulation Of Eeg Spectral Edge Frequency During Patterned Pneumatic Oral Stimulation In Preterm Infants, Dongli Song, Priya Jegatheesan, Sunshine Weiss, Balaji Govindaswami, Jingyan Wang, Jaehoon Lee, Austin Oder, Steven M. Barlow

Department of Special Education and Communication Disorders: Faculty Publications

Background—Stimulation of the nervous system plays a central role in brain development and neurodevelopmental outcome. Thalamocortical and corticocortical development is diminished in premature infants and correlated to electroencephalography (EEG) progression. The purpose of this study was to determine the effects of orocutaneous stimulation on the modulation of spectral edge frequency, f_c=90% (SEF-90) derived from EEG recordings in preterm infants.

Methods—Twenty two preterm infants were randomized to experimental and control conditions. Pulsed orocutaneous stimulation was presented during gavage feedings begun at around 32 weeks postmenstrual age (PMA). The SEF-90 was derived from 2-channel EEG recordings.

Results—Compared to the control …

The Impact Of Interface Design During An Initial High-Technology Aac Experience: A Collective Case Study Of People With Aphasia, Aimee R. Dietz, Kristy S.E. Weissling, Julie Griffith, Miechelle L. Mckelvey, Devan Macke

Department of Special Education and Communication Disorders: Faculty Publications

The purpose of this collective case study was to describe the communication behaviors of five people with chronic aphasia when they retold personal narratives to an unfamiliar communication partner using four variants of a visual scene display (VSD) interface. The results revealed that spoken language comprised roughly 70% of expressive modality units; variable patterns of use for other modalities emerged. Although inconsistent across participants, several people with aphasia experienced no trouble sources during the retells using VSDs with personally relevant photographs and text boxes. Overall, participants perceived the personally relevant photographs and the text as helpful during the retells. These …

Treating Myofunctional Disorders: A Multiple-Baseline Study Of A New Treatment Using Electropalatography, Alana Mantie-Kozlowski, Kevin M. Pitt

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: This study assessed the benefit of using electropalatography (EPG) in treatment aimed at habilitating individuals with nonspeech orofacial myofunctional disorders (NSOMD).

Method: The study used a multiple-baseline design across 3 female participants who were referred for an evaluation and possible treatment of their NSOMD. Treatment sessions were 30 min and provided twice weekly. Participant 1 received 8 treatments, Participant 2 received 6 treatments, and Participant 3 received 4 treatments. The patterns of sensor activation produced when participants’ tongues made contact with the electropalate during saliva swallows were compared with the patterns of age-matched peers. Individualized goals were developed on …

Phonological And Lexical Influences On Phonological Awareness In Children With Specific Language Impairment And Dyslexia, Kelly Farquharson, Tracy M. Centanni, Chelsea E. Franzluebbers, Tiffany Hogan

Department of Special Education and Communication Disorders: Faculty Publications

Children with dyslexia and/or specific language impairment have marked deficits in phonological processing, putting them at an increased risk for reading deficits. The current study sought to examine the influence of word-level phonological and lexical characteristics on phonological awareness. Children with dyslexia and/or specific language impairment were tested using a phoneme deletion task in which stimuli differed orthogonally by sound similarity and neighborhood density. Phonological and lexical factors influenced performance differently across groups. Children with dyslexia appeared to have a more immature and aberrant pattern of phonological and lexical influence (e.g., favoring sparse and similar features). Children with SLI performed …

A Longitudinal Examination Of The Measurement Properties And Predictive Utility Of The Center For Epidemiologic Studies Depression Scale Among North American Indigenous Adolescents, Brian E. Armenta, Kelley J. Sittner Hartshorn, Les B. Whitbeck, Devan M. Crawford, Dan R. Hoyt

Department of Sociology: Faculty Publications

We examined the longitudinal measurement properties and predictive utility of the Center for Epidemiologic Studies Depression Scale (CES-D) from early to late adolescence among a sample of North American Indigenous youths. Participants were 632 North American Indigenous adolescents (n = 632; 50.3% girls; M age at baseline = 11.11 years) participating in an 8-year, 8-wave longitudinal study. Via in-person interviews, participants completed the CES-D at Waves 1, 3, 5, and 7, and the major depressive disorder (MDD) module of the Diagnostic Interview Schedule for Children at Waves 1, 4, 6, and 8. Confirmatory factor analyses indicated that responses to the …

Tiers 2 & 3 Intervention: Corporal Punishment, A Traditional Discipline Consequence, Reece L. Peterson, Ann O'Connor

Department of Special Education and Communication Disorders: Faculty Publications

Conclusion

Corporal punishment is a controversial form of discipline that can evoke strong emotions from parents and the general public based on ethical and moral issues. In states that allow corporal punishment, it is important to make sure policies and procedures are in place to administer corporal punishment in order to let parents know what to expect with this form of discipline and to maintain legality of the procedures. Corporal punishment does in fact offer an alternative to exclusionary disciplinary consequences. As a result, schools where it is employed might have lower numbers of suspensions compared to schools where it …

Tier 3 Intervention: Discipline Recovery, Reece L. Peterson, Ann O'Connor, Jenna Strawhun

Department of Special Education and Communication Disorders: Faculty Publications

Conclusion

Clearly, further research on discipline recovery and education programs for students that have been expelled is warranted. Several states and programs appear to be making strides in providing students opportunities to accumulate credits and/or prepare for the workforce while expelled from public school, and clearly this is better than no such programs. However, there is little formal evidence to support or guide specific implementation or programming details for these types programs.

Animal Attendance At M-44 Sodium Cyanide Ejector Sites For Coyotes, John A. Shivik, Lauren Mastro, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Sodium cyanide (NaCN) ejectors for coyotes (Canis latrans), known as M-44s, are used in many parts of the United States for lethally removing coyotes to protect livestock or other resources. Quantifying selectivity of current devices in killing target and non-target species is important to users and provides a baseline for future development of more effective and selective techniques. We used motion-activated cameras to monitor M-44 locations for coyote and other species visitations to the sites. Because camera presence potentially influences coyote behavior, we first compared activation of M-44s at paired sites where cameras were and were not present. Coyotes activated …

Biology And Impacts Of Pacific Island Invasive Species. 11. Rattus Rattus, The Black Rat (Rodentia: Muridae), Aaron B. Shiels, William C. Pitt, Robert T. Sugihara, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

The black rat, roof rat, or ship rat (Rattus rattus L.) is among the most widespread invasive vertebrates on islands and continents, and it is nearly ubiquitous on Pacific islands from the equatorial tropics to approximately 55 degrees latitude north and south. It survives well in human-dominated environments, natural areas, and islands where humans are not present. Rattus rattus is typically the most common invasive rodent in insular forests. Few vertebrates are more problematic to island biota and human livelihoods than R. rattus; it is well known to damage crops and stored foods, kill native species, and serve as a …

Potential Citric Acid Exposure And Toxicity To Hawaiian Hoary Bats (Lasiurus Cinereus Semotus) Associated With Eleutherodactylus Frog Control, William C. Pitt, Gary W. Witmer, Susan M. Jojola, Hans Sin

United States Department of Agriculture Wildlife Services: Staff Publications

We examined potential exposure of Hawaiian hoary bats (Lasiurus cinereus semotus) to citric acid, a minimum risk pesticide registered for control of invasive Eleutherodactylus frog populations. Hoary bats are nocturnal insectivores that roost solitarily in foliage, federally listed as endangered, and are endemic to Hawaii. Oral ingestion during grooming of contaminated fur appears to be the principal route by which these bats might be exposed to citric acid. We made assessments of oral toxicity, citric acid consumption, retention of material on fur, and grooming using big brown bats (Eptesicus fuscus) as a surrogate species. We evaluated both ground application and …