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Articles 16111 - 16140 of 20604
Full-Text Articles in Entire DC Network
Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao
Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao
Turkish Journal of Biology
The objective of this study was to obtain the complete coding sequences (CDSs) of 3 buffalo genes (AKT1, AKT2, and AKT3) using reverse-transcriptase polymerase chain reaction and to depict their molecular characterizations and tissue expression patterns in buffalo. The buffalo AKT1, AKT2, and AKT3 CDSs were 1443 bp, 1446 bp, and 1440 bp in length and encoded 480, 481, and 479 amino acids, respectively. Nine, 13, and 3 nucleotide differences were found in the CDSs between buffalo and other bovine species. Phylogenetic analyses showed that buffalo AKT1 and AKT2 have close genetic relationships with other species in the family Bovidae, …
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Turkish Journal of Biology
Beta-galactosidases (\BETAgals) (EC 3.2.1.23) have been detected in a wide range of plant organs and tissues and are described by their ability to hydrolyze terminal nonreducing \BETA-D-galactosyl residues from \BETA-D-galactosides. In this study, 92 \BETAgal protein sequences from different plants, 7 animal samples including human and mouse, 3 samples from bacteria including Escherichia coli, and 4 samples from insects including Drosophila melanogaster were aligned. Sequences were analyzed by computational tools to predict the protein properties, such as molecular mass, isoelectric point, signal peptide, motifs, transmembrane domain, and secondary and spatial structure. Protein structure analysis revealed there is a high identity …
Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. A highly active antibacterial substance produced by Sm-Mm3 was purified by ammonium sulfate precipitation and high-performance liquid chromatography, respectively, and identified by spectrometric analysis (TOF-MS). Finally, we showed that this molecule is heat-stable, stable in the pH range of 5-9, and insensitive to organic solvents and proteinase K. The molecular mass was determined as 479 Da. Plasmid elimination demonstrated …
Serum Leptin Profiles, Food Intake, And Body Weight In Melatonin-Implanted Syrian Hamsters (Mesocricetus Auratus) Exposed To Long And Short Photoperiods, Bülent Gündüz
Turkish Journal of Biology
We have shown that circadian changes in leptin concentrations are inversely linked to circulating melatonin levels in the Syrian hamster. The present study aimed to assess the effects of subcutaneous melatonin implants under different photoperiodic conditions: did the implants affect serum leptin levels, body weight, food consumption, and/or testicular weight? Male hamsters were exposed to long and short photoperiods for 10 weeks and received subcutaneous melatonin implants (1 mg in 24 mg beeswax) every 2 weeks. Blood samples were collected every week at midday (1200 hours) or midnight (0000 hours) to assess leptin and melatonin concentrations. Testes were measured to …
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US$100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1^{pro1}, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1^{pro1} has a broad target pathogen range. In this study, we transformed …
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Turkish Journal of Biology
The aim of this study was to introduce antimicrobial activity to stainless steel orthodontic arch wires by coating them with TiO_2 in anatase form. Stainless steel (0.016 × 0.022 inch), D-rect (0.016 × 0.022 inch), and multistranded hammered retainer wires (0.014 × 0.018 inch) were coated with TiO_2 anatase by the sol-gel dip coating method. The wires were assessed for their photocatalytic antimicrobial activity against Streptococcus mutans, Candida albicans, and Enterococcus faecalis. After illumination under UVA (315-400 nm) at 1.0 mW/cm^2 for 1 h, the reduction efficiencies of the anatase-coated arch wires were calculated by using colony-forming unit counts. All …
Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao
Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao
Turkish Journal of Biology
Akirin2 is a recently discovered gene related to immune responses that also plays an important role in skeletal myogenesis. In this study, in order to scale up the production of recombinant porcine Akirin2 (pAkirin2), we report the expression and purification of a His-tagged version of recombinant pAkirin2 in Escherichia coli. The pAkirin2 is a polypeptide of 203 amino acids containing 42 rare codons. The recombinant pAkirin2 was expressed as an N-terminal fusion protein with His6 tag in E. coli Rosetta (DE3) host strain. The protein was purified by Ni-IDA affinity chromatography, yielding over 90% highly purified recombinant pAkirin2, with a …
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Turkish Journal of Biology
The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosome-specific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method. PCR products were separated by capillary electrophoresis and marker genotypes were determined. A total of 41 different alleles were observed. The mean allele number was 5.86 and mean F_{IS} value was 0.427 for all populations. The INRA189 locus was monomorphic for all populations except for South Anatolian Red. UMN0307 …
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Turkish Journal of Biology
Malva sylvestris is traditionally used for the treatment of liver diseases, but sufficient pharmacological-based scientific literature is not available online to authenticate its use in liver ailments. We aimed to assess the hepatoprotective effects of Malva sylvestris against paracetamol-induced hepatotoxicity in mice. The extract was concentrated using a rotary evaporator and then desired concentrations of extracts were made by dissolving in normal saline. The standard drug silymarin (100 mg/kg) was used as a reference drug to compare the therapeutic effects of Malva sylvestris. Two different doses of Malva sylvestris (300 and 600 mg/kg) were administered intraperitoneally for 7 consecutive days …
Development Of Durable Antimicrobial Surfaces Containing Silver- And Zinc-Ion-Exchanged Zeolites, Zeynep İyi̇gündoğdu, Selami̇ Demi̇rci̇, Nurcan Baç, Fi̇kretti̇n Şahi̇n
Development Of Durable Antimicrobial Surfaces Containing Silver- And Zinc-Ion-Exchanged Zeolites, Zeynep İyi̇gündoğdu, Selami̇ Demi̇rci̇, Nurcan Baç, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
The present work involves development of stable antimicrobial materials containing silver- and zinc-ion-exchanged zeolites. Faujasite X and Linde type A zeolites were synthesized, and following ion exchange with Ag^+ and Zn^{++} ions they were found to exhibit antimicrobial effects against bacteria (E. coli, P. aeruginosa, and S. aureus), yeast (C. albicans and C. glabrata), and fungi (A. niger and P. expansum). Zeolites-X and -A containing silver and zinc ions were then mixed with various coating materials, including paints and polypropylene, to develop antimicrobial composites. The long-term antimicrobial characteristics of zeolite-containing composite materials were investigated by inoculating selected microorganisms onto the …
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Turkish Journal of Biology
Kisspeptin signaling is a potent regulator of the reproductive axis and an elicitor of gonadotropin-releasing hormone secretion. The present study assessed the effect of the peripheral administration of a kisspeptin antagonist (P234) on testosterone secretion in the adult male rhesus monkey. Five monkeys were administered with either P234 (38.8 µg/kg body weight) or a vehicle (0.9% saline). Plasma testosterone and adiponectin levels, as a positive control, were then measured using specific ELISA assays from blood samples collected sequentially at 15-min intervals from 60 min prior to until 360 min after the P234/vehicle injection. In 3 monkeys, the experiment was repeated …
Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan
Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan
Turkish Journal of Biology
Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L^{-1} thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA) and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. The results showed that preconditioning followed by postconditioning on Murashige and Skoog (MS) medium with 0.25, 0.50, 0.75, and 1.0 mg L^{-1} 2iP or TDZ combined with 0.10 mg L^{-1} IBA …
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Turkish Journal of Biology
In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, …
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Turkish Journal of Biology
Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further. This study was initiated to develop an optimal in vitro germination medium for styrian oil pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) pollen, which would enable accurate germination testing. Different pH values and the addition of sucrose, mannitol, and polyethylene glycol to the Brewbaker and Kwack germination medium were tested. The …
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Turkish Journal of Biology
In human and animal organisms, lead can cause reproductive problems beginning with pregnant females. The reproductive axis is particularly sensitive to lead, its influence resulting in a delayed sexual maturity due to biosynthesis suppression of the sexual steroids. An animal model study was carried out on 28 white Wistar adult female rats, divided into 3 experimental (E) groups that were exposed for 12 months to lead acetate in drinking water as follows: 50 ppb Pb (E_1), 100 ppb Pb (E_2), and 150 ppb Pb (E_3), with a control group (M) that received unleaded tap water. Levels of FSH, LH, estradiol, …
Variation In Soil C And Microbial Functions Across Tree Canopy Projection And Open Grassland Microenvironments, Roberto Lai, Alessandra Lagomarsino, Luigi Ledda, Pier Paolo Roggero
Variation In Soil C And Microbial Functions Across Tree Canopy Projection And Open Grassland Microenvironments, Roberto Lai, Alessandra Lagomarsino, Luigi Ledda, Pier Paolo Roggero
Turkish Journal of Agriculture and Forestry
Mixtures of grasslands and perennial woody crops or vineyards represent a major source of potential carbon storage or release. Understanding the spatial variability of soil properties in these ecosystems is important in determining soil constraints related to the management of soil resources. The aims of the study were 1) to explore the spatial variability associated to the trees for soil C storage and its components and reactivity; and 2) to assess the similarities between microenvironments in terms of microbial functional diversity. Eight microenvironments characterized by different long-term soil management practices and different positions with respect to woody plant canopy soil …
Using Plant Extracts To Reduce Asexual Reproduction Of Apple Scab (Venturia Inaequalis), János Bálint, Szilveszter Nagy, Rezso Thiesz, Imre-István Nyárádi, Adalbert Balog
Using Plant Extracts To Reduce Asexual Reproduction Of Apple Scab (Venturia Inaequalis), János Bálint, Szilveszter Nagy, Rezso Thiesz, Imre-István Nyárádi, Adalbert Balog
Turkish Journal of Agriculture and Forestry
The aim our research was to develop effective methods for organic apple farming, based on the use of plant extracts to control the asexual reproduction of Venturia inaequalis. Plant extracts of artemisinin, chelidonine, thymol, and populin tested in vitro under laboratory conditions significantly slowed down the germination of V. inaequalis conidia. During a 2-year study under field conditions, the effect of 1% populin, an extract from black poplar buds, on apple scab was tested in parallel with synthetic fungicides and a no-pesticide regime on Golden Delicious and Jonathan apples. Populin significantly reduced the number of scabbed leaves and fruits on …
Aging And Cancer: Molecular Facts And Awareness For Turkey, Gülgün Gündüz, Kayahan Fişkin
Aging And Cancer: Molecular Facts And Awareness For Turkey, Gülgün Gündüz, Kayahan Fişkin
Turkish Journal of Biology
Two important subjects concerning extension of the human life-span are biological aging processes and their relationship to diseases, and especially to the formation of cancer. It is clear that an aging population poses some challenges for developing countries. We set out to show the demographic structure of Turkey and to discuss the feasibility of decreasing the prevalence of cancer. We overviewed the literature on the relationship between biological aging and cancer formation, the environmental causes of cancer, and age-related characteristics of cancer in the elderly. In addition, some statistical data on the changing demography of Turkey were reviewed. It is …
Autophagy And Cancer, Hacer Ezgi̇ Karakaş, Devri̇m Gözüaçik
Autophagy And Cancer, Hacer Ezgi̇ Karakaş, Devri̇m Gözüaçik
Turkish Journal of Biology
Autophagy is an evolutionary conserved intracellular degradation and stress response mechanism that is mainly responsible for the breakdown and recycling of cytoplasmic materials, including long-lived proteins, protein aggregates, and damaged organelles. In this way, autophagy provides the cell with building blocks and allows the maintenance of homeostasis under stress conditions such as growth factor deficiency, nutrient deprivation, hypoxia, and toxins. Consequently, abnormalities of autophagy contribute to a number of pathologies ranging from neurodegenerative diseases to cancer. Autophagy was reported to have a dual role in cancer. Depending on cancer stage, autophagy seems to act as tumor suppressor or as a …
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Turkish Journal of Biology
The leading cause of death in cancer patients is metastasis. Invasion is an integral part of metastasis and is carried out by proteolytic structures called invadopodia at the cellular level. In this introductory review, we start by evaluating the definition of invadopodia. While presenting the upstream signaling events involved, we integrate current models on invadopodia. In addition, we discuss the significance of invadopodia in 2D and 3D and in vivo. We finally point out technical challenges and conclude with open questions in the field.
It Takes 2 Antioxidants To Tango: The Interaction Between Manganese Superoxide Dismutase And Glutathione Peroxidase-1, Dede N. Ekoue, Alan M. Diamond
It Takes 2 Antioxidants To Tango: The Interaction Between Manganese Superoxide Dismutase And Glutathione Peroxidase-1, Dede N. Ekoue, Alan M. Diamond
Turkish Journal of Biology
Reactive oxygen species (ROS) are generated during mitochondrial oxidative metabolism. Accumulation of ROS without an effective antioxidant response can lead to oxidative stress, resulting in macromolecular damage that is implicated in the etiology of various diseases including cancer. ROS detoxification is regulated by various antioxidant proteins, specifically manganese superoxide dismutase (MnSOD), which catalyzes the conversion of superoxide into H_{2}O_{2}, and the subsequent conversion of H_{2}O_{2} into water is catalyzed by glutathione peroxidase 1 (GPx-1). In vitro and in vivo evidence supports a conflicting role of MnSOD in tumor biology and indicates that an interaction between MnSOD and GPx-1 can modulate …
Assessment Of Density-Dependent Feeding Damage By The Cotton Dusky Bug, Oxycarenus Laetus Kirby (Hemiptera: Lygaeidae), In Cotton, Muhammad Ahsan Khan, Muhammad Dildar Gogi, Muhammad Hamid Bashir, Mubasher Hussain, Zain-Ul Abdin, Muhammad Aamir Rashid
Assessment Of Density-Dependent Feeding Damage By The Cotton Dusky Bug, Oxycarenus Laetus Kirby (Hemiptera: Lygaeidae), In Cotton, Muhammad Ahsan Khan, Muhammad Dildar Gogi, Muhammad Hamid Bashir, Mubasher Hussain, Zain-Ul Abdin, Muhammad Aamir Rashid
Turkish Journal of Agriculture and Forestry
The dusky cotton bug, Oxycarenus laetus Kirby (Hemiptera: Lygaeidae), is a serious threat to cotton due to early cultivation of Bt cotton. These studies were carried out under laboratory and field conditions to estimate the feeding damage caused by O. laetus to seed weight, seed germination, and lint quality of cotton cultivar Bt-121 at 6 bug densities (0, 5, 10, 15, 20, and 25 adult pairs). The results revealed that seed germination and seed weight decreased, whereas percent reduction in seed germination and seed weight over control increased with increasing bug density. Bug density in the range of 5-25 pairs …
Antioxidant And Radical Scavenging Activities In Fruits Of 6 Sea Buckthorn (Hippophae Rhamnoides L.) Cultivars, Otakar Rop, Sezai̇ Erci̇şli̇, Jiri Mlcek, Tunde Jurikova, Ignac Hoza
Antioxidant And Radical Scavenging Activities In Fruits Of 6 Sea Buckthorn (Hippophae Rhamnoides L.) Cultivars, Otakar Rop, Sezai̇ Erci̇şli̇, Jiri Mlcek, Tunde Jurikova, Ignac Hoza
Turkish Journal of Agriculture and Forestry
Sea buckthorn (Hippophae rhamnoides L.) is a minor fruit crop. The most important breeding activities focused on the production of sea buckthorn fruits as a top-quality food product have been performed in Russia, Germany, and the Czech Republic. Over the past several decades, plant breeders have selected a number of cultivars that show excellent physical and nutritional properties. The aim of this study was to monitor and determine the total contents of phenols and flavonoids as well as antioxidant capacity and scavenging activity of methanolic extracts on reactive oxygen species and lipid peroxidation in some cultivars of sea buckthorn suitable …