Digital Commons Network^™

Split Inteins: From Mechanistic Studies To Novel Protein Engineering Technologies, Neel H. Shah

Student Theses and Dissertations

Inteins are auto-processing protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( external protein) regions through a native peptide bond. Remarkably, a small subset of all inteins are naturally transcribed and translated as two fragments that efficiently associate and carry out the same biochemical process in trans, and these split inteins are potentially powerful tools for protein engineering. Recently, a split intein from the cyanobacterium Nostoc punctiforme (Npu) was discovered that …

Transcriptome-Wide Characterization Of Apobec1-Catalyzed Rna Editing Events In Macrophages, Claire Ellen Hamilton

Student Theses and Dissertations

RNA editing refers to the process by which the sequence of RNA is altered through the insertion, deletion or modification of specific nucleotides. Editing of mRNA transcripts can increase the informational complexity encoded by the genome by producing alternative protein isoforms through specific posttranscriptional RNA editing events. Additionally, RNA editing in non-coding regions of mRNA transcripts has been shown to influence gene expression in a tissue-specific manner. In mammals, mRNA editing serves a diverse set of biological roles in neuronal function, host defense and lipid metabolism. The major mRNA editors acting in mammals include the adenosine deaminases acting on RNA …

Forces Generated By Cell Intercalculation Tow Epidermal Sheets In Mammalian Tissue Morphogenesis, Evan Heller

Student Theses and Dissertations

Underlying the dramatic tissue movements of development—the bending, folding, squeezing, pushing, pulling, mass movements, and individual movements—are processes of cell migration. Throughout our lives, cell migration plays a role in the maintenance and regeneration of our tissues, and even in the development and progression of disease. To carry out the complex tasks of development and tissue morphogenesis, cells must coordinate their behaviors and migrate collectively. Insights into these collective behaviors have come from elegant studies of gastrulation movements in model organisms such as flies, frogs, and fish, uncovering conserved cellular and molecular mechanisms. However, the extent to which these mechanisms …

Identification Of A Microrna Network That Regulates Melanoma Metastasis And Angiogenesis By Targeting Apoe, Nora Pencheva

Student Theses and Dissertations

Metastatic melanoma, a prevalent and deadly form of skin cancer, remains an aggressive clinical outcome that lacks effective preventative and/or curative therapeutics. Patients diagnosed with advanced metastatic melanoma face a dreaded 10-year survival rate of less than 10%. By performing in vivo selection for highly metastatic melanoma cells, we systematically identified three microRNAs (miRNAs) that promoted metastasis across multiple mutationally diverse human melanoma subtypes. These miRNAs were found to promote metastasis by enhancing the invasive capacity of melanoma cells and also by allowing melanoma cells to recruit endothelial cells into the metastatic niche, leading to enhanced metastatic angiogenesis. Importantly, the …

What Is A 'Religious Institution'?, Zoe Robinson

College of Law Faculty

Change in the First Amendment landscape tends towards the incremental, but the Supreme Court’s opinion two terms ago in Hosanna-Tabor v. EEOC — holding that religious institutions enjoy a range of First Amendment protections that do not extend to other individuals or organizations — is better understood as a jurisprudential earthquake. The suddenness and scale of the shift helps to explain the turmoil that has ensued in the lower courts and law journals. And yet, it could be that the biggest aftershock has yet to be felt. The Court left open the most important functional question that exists in scenarios …

Physical Environment And Women's Physical Activity Behaviors, Beth Ann Brisky

All Graduate Theses, Dissertations, and Other Capstone Projects

This study was developed using the Ecological Model (Stokols, 1992). The purpose of this study was to determine women's perceptions of the physical environment and the relationship between perceptions and physical activity behaviors. The study also analyzed women's non-motorized transportation behaviors, including walking and cycling to get from place to place. An email was sent to 526 female Minnesota State University, Mankato employees to ask them to participate in an electronic survey. The survey was developed using two of Sallis's (2013a, 2013b) instruments, Neighborhood Quality of Life Study survey and Neighborhood Environment Walkability Scale-Confirmatory Factor Analysis survey. The survey consisted …

Benchmarks 2014, January 31, The Rockefeller University

BenchMarks 2014

BenchMarks. Friday, January 31, 2014

The community newsletter of The Rockefeller University

BenchMarks is published monthly and is distributed on the campus of The Rockefeller University. It produced by the Office of Communications and Public Affairs

Utah Shrubland Management, Thomas A. Monaco, Kari E. Veblen, Justin Williams

Wildland Resources Faculty Publications

This Winter 2014-2015 Newsletter discusses accomplishments over the past year for the Shrubland Management project. We highlight the Ecological Sites and targeted shrubs at the ranch owned by Mr. Rial Berry in Cedar Fort, Utah.

Influence Of Group Size On The Success Of Wolves Hunting Bison, Daniel R. Macnulty, Aimee G. Tallian, Daniel R. Stahler, Douglas W. Smith

Wildland Resources Faculty Publications

An intriguing aspect of social foraging behavior is that large groups are often no better at capturing prey than are small groups, a pattern that has been attributed to diminished cooperation (i.e., free riding) in large groups. Although this suggests the formation of large groups is unrelated to prey capture, little is known about cooperation in large groups that hunt hard-to-catch prey. Here, we used direct observations of Yellowstone wolves (Canis lupus) hunting their most formidable prey, bison (Bison bison), to test the hypothesis that large groups are more cooperative when hunting difficult prey. We quantified the relationship between capture …

Effects Of Climatic Variation And Reproductive Trade-Offs Vary By Measure Of Reproductive Effort In Greater Sage-Grouse, Danny Caudill, Michael R. Guttery, Brent Bibles, Terry A. Messmer, Gretchen Caudill, Erin Leone, David K. Dahlgren, Renee Chi

Wildland Resources Faculty Publications

Research on long-lived iteroparous species has shown that reproductive success may increase with age, until the onset of senescence, and that prior reproductive success may influence current reproductive success. Such complex reproductive dynamics can complicate conservation strategies, especially for harvested species. Further complicating the matter is the fact that most studies of reproductive costs are only able to evaluate a single measure of reproductive effort. We systematically evaluated the effects of climatic variation and reproductive trade-offs on multiple reproductive vital rates for greater sage-grouse (Centrocercus urophasianus; sage-grouse), a relatively long-lived galliforme of conservation concern throughout western North America. Based on …

When Enough Is Enough: Location Tracking, Machine Learning And The Mosaic Theory, Renee Mcdonald Hutchins, Steve Bellovin, Tony Jebara, Sebastian Zimmeck

Journal Articles

Since 1967, when it decided Katz v. United States, the Supreme Court has tied the right to be free of unwanted government scrutiny to the concept of reasonable expectations of privacy.1 An evaluation of reasonable expectations depends, among other factors, upon an assessment of the intrusiveness of government action. When making such assessment historically the Court considered police conduct with clear temporal, geographic, or substantive limits. However, in an era where new technologies permit the storage and compilation of vast amounts of personal data, things are becoming more complicated. A school of thought known as “mosaic theory” has stepped into …

Medicine@Yale December 2014 Volume 10, Issue 3, Yale School Of Medicine Office Of Communications

Medicine@Yale - development newsletter

Medicine@Yale : advancing biomedical science, education and health care was a print and online newsletter which published news about academics, research and philanthropy for alumni, donors, faculty, students and the general public. It was initially published 6 times a year and then changed to a quarterly schedule. The first issue came out June/Jul. 2005. The final issue was published in May/June 2022, vol. 17, issue 1. Published by Yale School of Medicine, Office of Communications.

Key Roles Of Sub-Cellular Membranes And Co-Chaperone In Tombusvirus Replication, Kai Xu

Theses and Dissertations--Plant Pathology

Positive strand RNA viruses, inculding tombusviruses, are known to utilize cellular membranes to assemble their replicase complexes (VRCs). Two tombusviruses , Tomato bushy stunt virus (TBSV) and Carnation Italian ringspot virus (CIRV), replicate on different organellar membranes, peroxisomes or endoplasmic reticulum (ER) for TBSV and mitochodria outer membranes in case of CIRV. I showed that both TBSV and CIRV replicase proteins could assemble VRCs and replicate viral RNA on purified microsomes (ER) and mitochondria. Different efficiencies of assembly was shown determined by multiple domains on TBSV or CIRV replication proteins.

To study why VRC assembly could occur on an alternative …

Book Review: Court-Packing And Legal Creation - Symposium: History And Meaning Of The Constitution, Sheldon Gelman

Cleveland State Law Review

Review of FDR and Chief Justice Hughes: The President, the Supreme Court, and the Epic Battle over the New Deal by James F. Simon. New York: Simon & Schuster. 2012

Hurd Farm Llc: Resilience Through Redefined Production, Ruby Woodside

The Sustainability Institute Publications

No abstract provided.

Lobster Fishing: Shifting Cycles And Changing Traditions, Ruby Woodside

The Sustainability Institute Publications

No abstract provided.

Martha’S Vineyard Shellfish Group, Inc. Healthy Ecosystems For Coastal Resilience, Ruby Woodside

The Sustainability Institute Publications

No abstract provided.

Getting People To Make The Right Choice Under The Aca: The Most Important “Sales Pitch” Of Obama’S Presidency, Brietta R. Clark

Journal of Health Care Law and Policy

No abstract provided.

Mind The Gap: Basic Health Along The Aca’S Coverage Continuum, Sallie Thieme Sanford

Journal of Health Care Law and Policy

No abstract provided.

A Visit To The Kilns: A Formative Experience For Two Christian Teacher Educators, Karen Buchanan, Thomas D. Buchanan

Faculty Publications - College of Education

The C.S. Lewis Study Centre at the Kilns is located near Oxford, England in the previous home of C.S. Lewis, the highly influential 20th century Christian intellectual and apologist. Two professors, who coupled a brief stay at the Kilns with an inquiry into the life of Lewis and his writings, present insights into their work as teacher educators. Three main areas are explored. The first area is the nature of our work, including a discussion of the distinct roles associated with Christian teacher educators and the importance of professional community. Next is a discussion on the quality of our work, …

Kenyon College Alumni Bulletin - Winter 2014

Kenyon College Alumni Bulletin

No abstract provided.

Antipredator Behavior In Desmognathus Ochrophaeus: Threat-Specific Responses To Chemical Stimuli In A Foraging Context, Elyse C. Johnson, Aaron M. Sullivan

Faculty Publications - Department of Biological & Molecular Science

Prey species may reduce the likelihood of injury or death by engaging in defensive behavior but often incur costs related to decreased foraging success or efficiency. To lessen these costs, prey may adjust the intensity or type of antipredator behavior according to the nature of the perceived threat. We evaluated the potential for threat-sensitive responses by Allegheny Mountain dusky salamanders (Desmognathus ochrophaeus) exposed to chemical stimuli associated with predation by asking three questions:(1) Do individual D. ochrophaeus respond to chemical cues in a threat-sensitive manner? (2) Do salamanders exhibit the same pattern of behavioral response while foraging? and (3) Is …

The Decline Of Design Immunity: Impacts Of Martinez V. New Mexico Department Of Transportation On Traffic Control Device Analysis And Constrictive Notice Of A Dangerous Condition Of Public Roadways, Matthew A. Zidovsky

New Mexico Law Review

No abstract provided.

Swahili Word Order Choices: Insights From Information Structure, Mohamed Yusuf Mwamzandi

Linguistics & TESOL Dissertations - Archive

Cross-linguistic pragmatic studies have shown non-canonical word order can often be explained if information structure is taken into consideration. This dissertation explores word order variation in Swahili (SVO, Bantu) adnominal demonstratives and reciprocal constructions under the auspices of information structure. I investigate how the notion of topic may explain word order variation in these two structures.The term `adnominal demonstrative' refers to demonstratives that co-occur with nouns such as huyu mtu `this person' as distinguished from pronominal demonstratives such as huyu `this'. The issue to be determined is what influences the demonstrative use in pre or postnominal position. The data consists …

Trade Usage In The Courts: The Flawed Conceptual And Evidentiary Basis Of Article 2’S Incorporation Strategy, Lisa Bernstein

Public Law and Legal Theory Working Papers

No abstract provided.

Threats And Bullying By Prosecutors, Bennett L. Gershman

Elisabeth Haub School of Law Faculty Publications

Part I of this Essay describes ten contexts in which prosecutors make threats and behave like bullies. Some of these contexts are familiar, such as grand jury proceedings or plea discussions, where threats are generally upheld. Threats in other contexts are not as easy to justify, such as threats to obtain testimony from prosecution witnesses, retaliating for the exercise of constitutional rights, forcing a waiver of civil rights claims, and publicly humiliating people. Other threats clearly are illegitimate and unethical, such as threats that drive defense witnesses off the stand, bringing criminal charges against outspoken critics and defense experts, and …

Meat Animals, Humane Standards And Other Legal Fictions, David N. Cassuto

Elisabeth Haub School of Law Faculty Publications

Law and food are distinct concepts, though the discipline (Law and Food) implies a relationship worthy of study. The conjunction (“and”) creates meaning. However, its absence also conveys meaning. For example, “meat animal” suggests that animals can be both meat and animal. This conflation has powerful legal implications. National Meat Association v. Harris (2012) makes chillingly plain the law’s indifference to whether a meat animal is alive or dead. This essay examines the way supposedly humane federal practices ignore the systematic brutalization of “food animals” as those animals get processed into marketable flesh. It concludes with some observations about why …

The Charter Of The Forest: Evolving Human Rights In Nature, Nicholas A. Robinson

Elisabeth Haub School of Law Faculty Publications

Carta de Foresta, the Charter of the Forest of 1217, is among the first statutes in environmental law of any nation. Crafted to reform patently unjust governance of natural resources in 13th century England, the Charter of the Forest became a framework through which to reconcile competing environmental claims, then and into the future. The Charter confirmed the rights of “free men.” Kings resisted conceding these rights. When confronted with violation of the Charter, barons and royal councils obliged kings repeatedly to reissue the Forest Charter and pledge anew to obey its terms.

Setting The Table For Urban Agriculture, Margot J. Pollans

Elisabeth Haub School of Law Faculty Publications

This article provides context for the various roles that law plays in the cultivation of urban agriculture. This article first reflects on how popular support for the development of a legal framework that promotes urban agriculture is rooted deeply in American agrarian traditions. The article then notes the palatable tension between the rhetoric in support of urban agriculture and the modes of urban law and planning that dominated the twentieth century. It considers how various approaches to urban planning have facilitated or thwarted urban agriculture and surveys recent legal developments designed to accommodate and encourage urban agriculture projects as alternatives …

Winter 2014

Metro Denver Dental Society Articulator Magazine

The MDDS Articulator magazine has been serving as the voice for Denver's dental community for over 100 years.

Distributed to approximately 2,000 dental professionals quarterly, the Articulator features local news, industry trends, practice management guidance, data-driven research and more. The award-winning publication is available to member dentists in both print and digital forms.

Issues include cover and feature articles alongside regular columns with regional news impacting the Metro Denver membership including editorial and perspectives columns, legal, association activities, component news, continuing education opportunities, and classifieds.