Digital Commons Network^™

Near-Infrared Imaging Of Adoptive Immune Cell Therapy In Breast Cancer Model Using Cell Membrane Labeling, Fatma M. Youniss, Gobalakrishnan Sundaresan, Laura J. Graham, Li Wang, Collin R. Berry, Gajanan K. Dewkar, Purnima Jose, Harry D. Bear, Jamal Zweit

Radiology Publications

The overall objective of this study is to non-invasively image and assess tumor targeting and retention of directly labeled T-lymphocytes following their adoptive transfer in mice. T-lymphocytes obtained from draining lymph nodes of 4T1 (murine breast cancer cell) sensitized BALB/C mice were activated in-vitro with Bryostatin/Ionomycin for 18 hours, and were grown in the presence of Interleukin-2 for 6 days. T-lymphocytes were then directly labeled with 1,1-dioctadecyltetramethyl indotricarbocyanine Iodide (DiR), a lipophilic near infrared fluorescent dye that labels the cell membrane. Assays for viability, proliferation, and function of labeled T-lymphocytes showed that they were unaffected by DiR labeling. The DiR …

Art In The Age Of Networks - Networks As A Way Of Thinking, Rabeya Jalil

Higher Education Resources

The theme-based and material-based units (with lessons and lesson sequences) propose a curriculum for one academic semester in an undergraduate visual arts school (for sophomore, 2nd year, or junior, 3rd year students). However the lessons could be modified and tailored to any age group developmentally. This curricular framework aims to foster collaboration (within individuals, materials and disciplines), explore networked pedagogy and networks in pedagogy as a collaborating force through and with the visual arts and explore the materiality of the code and the digital media. The course also engages with new media theory and literature, investigates the materiality of the …

Rock Inhibitor Is Not Required For Embryoid Body Formation From Singularized Human Embryonic Stem Cells, Giuseppe Pettinato, Wendy S. Vanden Berg-Foels, Ning Zhang, Xuejun Wen

Chemical and Life Science Engineering Publications

We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCKi/+spin, and -ROCKi/-spin. Cell suspensions of BG01V/hOG and H9 hESC lines were pipetted into non-adherent hydrogel substrates containing defined microwell arrays. hEBs of consistent size and spherical geometry can be formed in each of the four conditions, including the -ROCKi/-spin condition. The hEBs formed under the -ROCKi/-spin condition differentiated to develop the three embryonic germ layers and tissues derived from …

Preliminary Study Of Kleptoplasty In Foraminifera Of South Carolina, Shawnee Lechliter

Bridges: A Journal of Student Research

Recent studies of living foraminifera, microscopic aquatic protists, indicate that some species have the ability to steal photosynthetic plastids from other microorganism and keep them viable through a process called kleptoplasty. Studying the symbiotic relationships within these diverse protists gives insight not only into evolutionary history, but also their importance to the ecosystem. We determined the presence of these kleptoplastic species and identified presence and origin of sequestered plastids based on morphological identification and molecular data from samples collected at Waties Island, South Carolina. We identified two kleptoplastic genera (Elphidium and Haynesina) and two non-kleptoplastic genera (Ammonia and Quinqueloculina) present …

2014 Celebration Of Inquiry Program, Coastal Carolina University

Celebration of Inquiry

11th Celebration of Inquiry, March 31-April 3, 2014. Theme: The Human Spirit: Overcoming Adversity to Accomplish Greatness. 6th Annual Undergraduate Research Competition abstracts and schedule are included in the program.

Relationship Between Canopy Location And Tensile Strength Of Leave Of Heteromeles Arbutifolia In Malibu, Ca, Elena Witham, Melinda Marchiano, Danielle Reynolds

Featured Research

Our hypothesis is that canopy location has an effect on the characteristic tensile strength of leaves, with the leaves on the top of the canopy having greater strength than the leaves on the bottom. We tested our experiment by sampling leaves from the top and bottom of three H. arbutifolia specimens, obtaining raw measurements of size, and testing the tensile strength of each leaf using the Instron. We performed t-tests on the data to determine if the average length, width, thickness, and tensile strength were statistically different depending on if our p values were < 0.05 when top and bottom leaves of the same tree were compared. We chose a chapparral stand removed from heavy human traffic and influence, eliminating as many extraneous variables as possible by taking leaves from the same tree of the same age from the same position on the branch and chose three trial specimen of similar height in the same area to reduce the possibility of varying sunlight exposure or water supply from affecting leaf morphology in a way that canopy position would not. The results show that blade length between top and bottom is statistically significantly different, while blade thickness and lateral strength at midsection were not statistically different. From our analysis of the data, it is clear that leaves on the bottom of the plant were longer and broader than leaves on the top, but we determined that canopy location does not have a major effect on tensile strength or thickness, supporting a null hypothesis.

Relationship Between Canopy Location And Tensile Strength Of Leaves Of Heteromeles Arbutifolia In Malibu, Ca, Elena Witham, Melinda Marchiano, Danielle Reynolds

Featured Research

Our hypothesis is that canopy location has an effect on the characteristic tensile strength of leaves, with the leaves on the top of the canopy having greater strength than the leaves on the bottom. We tested our experiment by sampling leaves from the top and bottom of three H. arbutifolia specimens, obtaining raw measurements of size, and testing the tensile strength of each leaf using the Instron. We performed t-tests on the data to determine if the average length, width, thickness, and tensile strength were statistically different depending on if our p values were < 0.05 when top and bottom leaves of the same tree were compared. We chose a chapparral stand removed from heavy human traffic and influence, eliminating as many extraneous variables as possible by taking leaves from the same tree of the same age from the same position on the branch and chose three trial specimen of similar height in the same area to reduce the possibility of varying sunlight exposure or water supply from affecting leaf morphology in a way that canopy position would not. The results show that blade length between top and bottom is statistically significantly different, while blade thickness and lateral strength at midsection were not statistically different. From our analysis of the data, it is clear that leaves on the bottom of the plant were longer and broader than leaves on the top, but we determined that canopy location does not have a major effect on tensile strength or thickness, supporting a null hypothesis.

Enzymatic Activity In The Chorion For Hatching In The California Grunion, Emily R. Pierce, Vince V. Quach, Karen L. Martin

Featured Research

Leuresthes tenuis is a small, silverside fish that spawns on the beaches during some of the highest tides of the summer months. Of the many unique traits to the species including that the eggs develop fully out of water to the point of hatching competence but will not hatch until presented with an environmental cue, which causes them to hatch in less than a minute. The purpose of this study is to better understand the role of enzymes called chorionases, which act to break down the chorion (egg membrane). I hypothesize that the chorion begins to weaken in this species …

Practice Patterns Of Equine Assisted Psychotherapists, Haley Gresham

Theses and Dissertations--Family Sciences

Modeled after Doherty & Simmons’ (1996) study on the practice patterns of marriage and family therapists, this project explored similar questions about equine assisted/facilitated psychotherapists who are members of two main certification organizations, EAGALA and PATH Intl. An Internet survey distributed to equine assisted/facilitated practitioners across the country explored demographic information of the clinician, their clients, and their typical work setting. Equine assisted/facilitated psychotherapy is a growing field and gaining an understanding of the current position of the field allows for growth in areas that are currently underdeveloped within this sector of the mental health field.

Renal Dysplasia, Halana V. Whitehead

Kidneycentric

No abstract provided.

Morquio A Syndrome-Associated Mutations: A Review Of Alterations In The Galns Gene And A New Locus-Specific Database, A Morrone, A Caciotti, R Atwood, K Davidson, C Du, Patricia Francis-Lyon, P Harmatz, M Mealiffe, S Mooney, T R. Oron, A Ryles, K A. Zawadzki, N Miller

Nursing and Health Professions Faculty Research and Publications

Morquio A syndrome (mucopolysaccharidosis IVA) is an autosomal recessive disorder that results from deficient activity of the enzyme N-acetylgalactosamine-6-sulfatase (GALNS) due to alterations in the GALNS gene, which causes major skeletal and connective tissue abnormalities and effects on multiple organ systems. The GALNS alterations associated with Morquio A are numerous and heterogeneous, and new alterations are continuously identified. To aid detection and interpretation of GALNS alterations, from previously published research, we provide a comprehensive and up-to-date listing of 277 unique GALNS alterations associated with Morquio A identified from 1,091 published GALNS alleles. In agreement with previous findings, most reported GALNS …

Naturalizing Immigration Imprisonment, César Cuauhtémoc García Hernández

Sturm College of Law: Faculty Scholarship

Only recently has imprisonment become a central feature of both t across every level of government and involving civil and criminal law enforcement tools.

Examining the population as a whole provides crucial insights as to how we arrived at this state of mass immigration imprisonment. While political motivations — parallel to those that fueled the rapid expansion of criminal mass incarceration — may have started the trend, this Article demonstrates that key legal and policy choices explain how imprisonment has become an entrenched feature of immigration law enforcement. In fact, legislators and immigration officials have locked themselves into this choice, …

Complement Expression In The Retina Is Not Influenced By Short-Term Pressure Elevation, Konstantin Astafurov, Cecilia Q. Dong, Lampros Panagis, Gautam Kamtham, Lizhen Ren, Anna Rozenboym, Tarique D. Perera, Jeremy D. Coplan, John Danias

Publications and Research

Purpose: To determine whether short-term pressure elevation affects complement gene expression in the retina in vitro and in vivo.

Methods: Muller cell (TR-MUL5) cultures and organotypic retinal cultures from adult mice and monkeys were sub- jected to either 24-h or 72-h of pressure at 0, 15, 30, and 45 mmHg above ambient. C57BL/6 mice were subjected to microbead-induced intraocular pressure (IOP) elevation for 7 days. RNA and protein were extracted and used for analysis of expression levels of complement component genes and complement component 1, q subcomponent (C1q) and comple- ment factor H (CFH) immunoblotting. …

Suffolk Journal, Vol. 74, No. 16, 2/12/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Annual Report Of The Town Officers For The Fiscal Year Ending December 31, 2013., Alexandria Town Representatives

Alexandria, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Suffolk Journal, Vol. 74, No. 19, 3/5/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Suffolk Journal, Vol. 75, No. 4, 9/24/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Suffolk Journal, Vol. 75, No. 5, 10/1/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Suffolk Journal, Vol. 75, No. 9, 10/29/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Suffolk Journal, Vol. 75, No. 11, 11/12/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Suffolk Journal, Vol. 75, No. 7, 10/15/2014, Suffolk Journal

Suffolk Journal

No abstract provided.

Parnassus 2014

Parnassus

The 2014 edition of the student literary journal, Parnassus, published by Taylor University in Upland, Indiana.

Donut Hole Summer 2014, Medicare Donations & Design Committee

Donut Hole Newsletter

No abstract provided.

Fy 2014 Annual Report, Tennessee. Division Of Forestry, Tennessee. Department Of Agriculture

Forestry Annual Reports

This annual report presents an overview of Tennessee Forestry Commission programs and management.

Dana Harel At The Palo Alto Art Center, John Zarobell

Global Studies Faculty Publications

No abstract provided.

Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to …

Interlinking Of Hypoxia And Estrogen In Thyroid Cancer Progression, S Rajoria, E Hanly, A Nicolini, A L. George, J Geliebter, E J. Shin, R Suriano, A Carpi, R K. Tiwari

NYMC Faculty Publications

Estrogen aids in neo-vascularization of various tumors during hypoxic conditions, however the role of estrogen within the hypoxic environment of thyroid cancer is not known. In a series of experimentations, using human thyroid cancer cells, we observed that estrogen and hypoxia modulate the hypoxia inducible factor-1 (HIF-1) signaling which is abrogated by the anti-estrogen fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole). Furthermore, we found that the conditioned medium from estrogen treated thyroid cancer cells lead to enhanced migration and tubulogenesis of human umbilical vein endothelial cells (HUVECs) which is abrogated by HIF-1 inhibitor. These findings, in addition to our previous …

Preliminary Report On The 2013 Field Season Of The American Excavations At Morgantina: Contrada Agnese Project (Cap), Alex Walthall, Randall Souza, Jared Benton, James F. Huemoeller

Art Faculty Publications

This article provides a preliminary report on the 2013 excavations carried out by the American Excavations at Morgantina (Sicily): Contrada Agnese Project (CAP). The 2013 season marked the start of this multiyear research and excavation project aimed at investigating both the urban planning of the city and the lives of its residents, with a specific focus on the periods of occupation and cultural transformation from the third to first century BCE. During the first season, three trenches were excavated in two parts of the ancient city. Their locations were chosen, in part, based on the results of a geophysical survey, …

Beneficial Assessment Outcomes From Frequent Testing, Abdulrazaq Imam

Psychology

When faced with deadlines, people tend to procrastinate. Students do this by delaying study time until examinations are so close the only option left is cramming. This procrastination scallop is a well-established behavioral phenomenon in both human and infrahuman species. Distributed practice also has been demonstrated to be superior to massed practice in the cognitive literature. Frequent testing provides opportunities for distributed practice and rehearsals that fill the gap between acquisition and the big test, creating its own mini-scallops. In sections of Introductory Psychology, Research Design, and Learning and Behavior courses, standard pre-post testing was conducted at the start and …

The Helminthological Society Of Washington 2013 Anniversary Award: Larry S. Roberts, Sherman S. Hendrix

Center for Brain, Biology, and Behavior: Faculty Publications

Dr. Roberts was born in the great state of Texas, and much of his early life is murky, but snakes are often mentioned. Larry received his Bachelor’s degree at Southern Methodist University; his Master of Science at the University of Illinois; and his Doctorate in the Department of Pathobiology at the Johns Hopkins School of Hygiene and Public Health. Larry’s doctoral research, published in Experimental Parasitology, documented the early development and crowding effect of the tapeworm, Hymenolepis diminuta, in the rat small intestine. His publication on this subject opened the door to the golden age of cestode physiology and biochemistry …