Digital Commons Network^™

Pathology Associated With Aav Mediated Expression Of Beta Amyloid Or C100 In Adult Mouse Hippocampus And Cerebellum, Eleanor S. Drummond, Jill Muhling, Ralph Martins, Linda K. Wijaya, Erich M. Ehlert, Alan R. Harvey

Research outputs 2013

Accumulation of beta amyloid (Ab) in the brain is a primary feature of Alzheimer’s disease (AD) but the exact molecular mechanisms by which Ab exerts its toxic actions are not yet entirely clear. We documented pathological changes 3 and 6 months after localised injection of recombinant, bi-cistronic adeno-associated viral vectors (rAAV2) expressing human Ab40- GFP, Ab42-GFP, C100-GFP or C100V717F-GFP into the hippocampus and cerebellum of 8 week old male mice. Injection of all rAAV2 vectors resulted in wide-spread transduction within the hippocampus and cerebellum, as shown by expression of transgene mRNA and GFP protein. Despite the lack of accumulation of …

The Guinea Pig As A Model For Sporadic Alzheimer's Disease (Ad): The Impact Of Cholesterol Intake On Expression Of Ad-Related Genes, Mathew Sharman, Seyyed H.M. Nik, Mengqi Chen, Daniel Ong, Linda K. Wijaya, Simon Laws, Kevin Taddei, Morgan Newman, Michael Lardelli, Ralph Martins, Guiseppe Verdile

Research outputs 2013

We investigated the guinea pig, Cavia porcellus, as a model for Alzheimer’s disease (AD), both in terms of the conservation of genes involved in AD and the regulatory responses of these to a known AD risk factor - high cholesterol intake. Unlike rats and mice, guinea pigs possess an Ab peptide sequence identical to human Ab. Consistent with the commonality between cardiovascular and AD risk factors in humans, we saw that a high cholesterol diet leads to up-regulation of BACE1 (b-secretase) transcription and down-regulation of ADAM10 (a-secretase) transcription which should increase release of Ab from APP. Significantly, guinea pigs possess …

Environmental And Conservation Volunteering As Workplace Integrated Learning For University Students, Rowena H. Scott, Eddie Van Etten

Research outputs 2013

This research paper introduces the concept and practice of tertiary sciences students doing environmental volunteering, also known as conservation volunteering, as a core part of their course. First year Natural Sciences students at Edith Cowan University do five days environmental volunteer work with community groups as a practicum, currently known as Workplace Integrated Learning (WIL). Initial research data displays the number of volunteer hours done by students in various types of activities, locations and organisations. Preliminary quantitative evaluations and qualitative comments demonstrate students’ positive attitudes and outcomes from their volunteering experiences. Definitions and classifications of volunteering and WIL from current …

What Bothers Australian Kids Online? Children Comment On Bullies, Porn And Violence, Lelia Green, Danielle Brady, Donell Holloway, Elisabeth Staksrud, Kjartan Ólafsson

Research outputs 2013

This briefing on what bothers Australian kids online builds upon a short report from the EU Kids Online network: In their own words: What bothers children online? Based upon research across 25 European nations, with 25,142 children (aged 9-16) and the parent or caregiver most involved in supporting the child’s internet use, the In their own words report addresses children’s answers to the question: ‘What things on the internet would bother people about your age?’ Children had not been asked about troubling content at this stage in the research, so their open-ended answers to this question represent the issues and …

Variability In The Carbon Storage Of Seagrass Habitats And Its Implications For Global Estimates Of Blue Carbon Ecosystem Service, Paul Lavery, Miguel-Angel Mateo, Oscar Serrano, Mohammad Rozaimi

Research outputs 2013

The recent focus on carbon trading has intensified interest in ‘Blue Carbon’–carbon sequestered by coastal vegetated ecosystems, particularly seagrasses. Most information on seagrass carbon storage is derived from studies of a single species, Posidonia oceanica, from the Mediterranean Sea. We surveyed 17 Australian seagrass habitats to assess the variability in their sedimentary organic carbon (Corg) stocks. The habitats encompassed 10 species, in mono-specific or mixed meadows, depositional to exposed habitats and temperate to tropical habitats. There was an 18-fold difference in the Corg stock (1.09– 20.14 mg Corg cm23 for a temperate Posidonia sinuosa and a temperate, estuarine P. australis …

Self-Assessment Of Employability Skill Outcomes Among Undergraduates And Alignment With Academic Ratings, Denise Jackson

Research outputs 2013

Despite acknowledgement of the benefits of self-assessment in higher education, disparity between student and academic assessments, with associated trends in over- and underrating, plagues its meaningful use, particularly as a tool for formal assessment. This study examines self-assessment of capabilities in certain employability skills in more than 1000 Australian business undergraduates. It evaluates the extent to which student self-assessments differ from academics, in what ways and the influence of certain individual and background characteristics - such as stage of degree, gender and academic ability - on rating accuracy. Explanations for documented disparities are presented, in addition to implications and strategies …

The Contribution Of Work-Integrated Learning To Undergraduate Employability Skill Outcomes, Denise Jackson

Research outputs 2013

WIL has attracted considerable attention as an instrument for enhancing professional practice and developing work-readiness in new graduates. It is widely considered as a point of difference in developing graduate employability by enhancing skill outcomes, such as team-work, communication, self-management and problem solving, employment prospects and student understanding of the world-of-work. This paper investigates the role of WIL in improving undergraduate employability skills; gauging its impact on a range of skills; and identifying variations in outcomes for certain demographic, study background and placement characteristics using survey data from 131 WIL students in an Australian university. Results indicate a significant improvement …

Student Perceptions Of The Importance Of Employability Skill Provision In Business Undergraduate Programs, Denise Jackson

Research outputs 2013

Studies examining student perceptions of employability skill development in business undergraduate programs are limited. Assurance of student buy-in is important to ensure learners engage with skill provision; to enable them to articulate their capabilities to potential employers and to facilitate the transfer of acquired skills. This study examines 1019 students’ perceptions of the importance of employability skill development, the relative importance of skills and the influence of certain demographic/background characteristics. Findings indicate undergraduates value skill development, most particularly communication and team-working, and some significant variations in importance ratings. Alignment with other stakeholder perceptions and the influence of context are discussed.

The Acceleration Of Aging And Alzheimer’S Disease Through The Biological Mechanisms Behind Obesity And Type Ii Diabetes, Ian J. Martins, Wei Ling F. Lim, Andrea C. Wilson, Simon M. Laws, Ralph Martins

Research outputs 2013

The incidence of diabetes is predicted to increase to 21% by 2050. Currently, one third of US adults are obese and over 11% of these individuals have diabetes. Due to the growing need for therapeutic intervention to control and/or stabilize this increase in the incidence of diabetes in Western communities, gaining a comprehensive understanding of the association between obesity and Type 2 diabetes has become increasingly important to diabetes research. The increased cell senescence associated with diabetes has been associated with the limited ability of cells to divide, with indication of telomere shortening and genomic instability of the cells. Obese …

Increased Risk For Obesity And Diabetes With Neurodegeneration In Developing Countries [Journal Article], Ian J. Martins

Research outputs 2013

The incidence of global obesity and Type 2 diabetes has increased and is predicted to rise to 30% of the global population. Diet and lifestyle factors are incapable to resolve the increased incidence for obesity and diabetes in various populations of the world. Developing countries have come to the forefront because of the higher diabetic epidemic. The urbanization may possibly provide an explanation for the global diabetic epidemic. In Western countries the metabolic syndrome and non alcoholic fatty liver disease (NAFLD) have reached 30 % of the population and now at present NAFLD afflicts 20% of developing populations. Western diets …

Exploring Ways To Improve Online Health News Stories, Trevor A. Cullen

Research outputs 2013

For a long time, reporting health consisted largely of statistics on the number of deaths and cases of disease, or reporting on epidemiological data that affects people we do not know. While this is important for health officials, it is of little interest to audiences who are increasingly demanding information that is useful to their daily lives. And conserving one’s health is perhaps the most useful of all topics. Many have now added the internet to their personal health toolbox, helping them to gain a better understanding of an illness or medical condition. But how accurate and balanced is the …

Identity On The Line: The Meaning Of Aboriginal And Non-Aboriginal Youth Hanging Out On The Streets And Train Stations In The South-East Corridor Of Perth, Andrew Guilfoyle, Sasha Botsis

Research outputs 2013

Executive Summary

We performed a critical analysis on data originally collected from young people, service providers, and parents, contained in Save the Children’s Photovoice and Participatory Action Research projects.

We employed an interpretative phenomenological approach with thematic analysis examining participants’ ascribed meaning, personal experiences, and observations of youth ‘hanging out’ on the streets or trains in the South-East corridor of Perth.

Criminology and Identity themes emerged, and we drew attention to the merits of a positive, holistic view of youth, growing up in today’s society; for the development of social programs, future interventions, and participatory action research.

We identify several …

Information Behavior And Japanese Students: How Can An Understanding Of The Research Process Lead To Better Information Literacy?, Yusuke Ishimura

Research outputs 2013

Academic librarians are striving to better serve international students as this emerging population grows on university campuses. Past studies of international students generally focus on linguistic and cultural differences in relation to information literacy skills development. However, it is necessary to go beyond these factors to better serve the population. By using a framework based in information behavior research, the present study investigates the factors that mediate students' behavior and information literacy skills. Practical recommendations based on the findings of an in-depth qualitative study of students' research process are provided.

Roles Of Compressive Residual Stress In Enhancing The Corrosion Resistance Of Nano Nitride Composite Coatings On Steel, Xiaoli Zhao, Paul Munroe, Daryoush Habibi, Zonghan Xie

Research outputs 2013

Nanocomposite coatings were synthesized, and subsequently annealed, in an attempt to understand the influence of residual stress upon their resistance to corrosion under acidic attack. Pitting corrosion, originating from microscopic coating defects, was commonly identified in the coatings subject to annealing. Finite element analysis was used to map the residual stress distribution adjacent to these defects and quantify resulting geometrical changes. The results show that with the presence of compressive residual stress in the coatings, corrosion-initiated cracking along weak interfaces was suppressed, and structural integrity maintained. Moreover, compressive residual stress helped constrict pre-existing structural defects in columnar-structured coatings and, in …

Investigation Of Mercury Use, Release, Deposition, And Exposures In The Tampa Bay Area, Ryan Algernon Michael

USF Tampa Graduate Theses and Dissertations

I investigate the links between mercury use, release, deposition, and population exposure in Tampa Bay, with the focus of identifying levers for reducing population mercury exposures. To achieve this, I investigated the trends in mercury use and release by products and processes in the Tampa Bay area using a Material Flow Analysis. Analysis of USEPA National Emissions Inventory data over time (1999 - 2008) identified relevant air source emission categories, and explored and compared state and regional trends in mercury emissions. To understand source contributions to wet deposited mercury in the Tampa Bay area, I analyzed trends in mercury deposition …

Unearthing Augusta: Landscapes Of Royalization On Roatan Island, Honduras, Lorena Diane Mihok

USF Tampa Graduate Theses and Dissertations

In 1742, the settlement of Augusta was established as an outpost of English royalization on Roatán Island, Honduras. This military camp housed a mix of English soldiers, English colonists, and local indigenous Miskitu peoples. While the settlement was occupied for only a brief span of seven years, the material record of the community provides insight into Miskitu-English interactions during the royalization process. Royalization encompassed strategies deployed by the English Crown to bring about loyalty to the state. In this dissertation, I discuss the concept of royalization from an agent-centered perspective to consider the intentions behind the occupants' usage of objects …

Translation, Adaptation And Invariance Testing Of The Teaching Perspectives Inventory: Comparing Faculty Of Malaysia And The United States, Jecky Misieng

USF Tampa Graduate Theses and Dissertations

As a result of growing attention in cross-cultural research, existing measurement instruments developed in one language are being translated and adapted for use in other languages and cultural contexts. The benefits of having the same instrument across cultures can only be realized if the process of translation and adaptation of the measurement instruments produces measurement operations that function similarly across national and cultural boundaries. Producing invariant measurement instruments that assess educational and psychological constructs provide a way of testing the cross-cultural generality of theories that include these constructs.

The major purposes of the study were to translate and adapt the …

Development Of Improved Models For Gas Sorption Simulation, Keith Mclaughlin

USF Tampa Graduate Theses and Dissertations

Computational chemistry offers one the ability to develop a better understanding of the complex physical and chemical interactions that are fundamental to macro- and mesoscopic processes that are seen in laboratory experiments, industrial processes, and ordinary, everyday life. For many systems, the physics of interest occur at the molecular or atomistic levels, and in these cases, computational modeling and two well refined simulation techniques become invaluable: Monte Carlo (MC) and molecular dynamics (MD). In this work, two well established problems were tackled. First, models and potentials for various gas molecules were produced and refined from first principles. These models, although …

Evaluating Habitat Vulnerability And Sustainability Of Urban Seagrass Resources To Sea Level Rise, Cynthia A. Meyer

USF Tampa Graduate Theses and Dissertations

The seagrass resource provides essential ecosystem functions for many marine species. This research evaluated the vulnerability and sustainability of the seagrass resource in an urbanized area to the effects of sea level rise. The assessment required analysis of information regarding the biogeography of the seagrass resource, and developing a method to model the spatial extent of the suitable habitat for seagrass, and applying the model to predict the implications of simulated sea level rise scenarios on the seagrass resource.

Examining the biogeography of the seagrass resource required the development of a seagrass monitoring and assessment field survey and a comprehensive …

Synthesis Of Titanium Dioxide Photocatalyst With The Aid Of Supercritical Fluids, Haitao Li

USF Tampa Graduate Theses and Dissertations

Titanium Dioxide (TiO2) emerged as one of the most popular photocatalysts since 1970's. However, its photocatalytic activity requires UV irradiation due to its large band gap unless further functionalization or modifications are performed. Furthermore, recovery issue has always been a major drawback, if the more effective form nano particles are utilized.

The key objectives of this research were synthesizing new TiO2 based photocatalyst systems that are effective with both the UV and the visible light while utilizing novel superior environmentally friendly techniques enabling development of nano-structured photocatalysts that can be easily recovered. In this dissertation research, highly porous nano-structured TiO2/WO3/Fe3+ …

A Case Study Of Jamaican Children's Lived Play Experiences, Carol Ann Long

USF Tampa Graduate Theses and Dissertations

Although research on children's play is abundant and considerable advances have been made in young children's play, the majority of these studies have been based in western developed countries and written from adults' perspectives rather than with children. Additionally, very little research has been done on children's play with active participants from smaller developing countries. The voices of society's youngest members have been lost or are only marginally represented.

The purpose of this qualitative research is to explore, understand, and describe young Jamaican children's lived play experiences as related through their eyes. The theoretical frameworks used to guide this study …

Growth And Herbivory Of The Black Mangrove, Avicennia Germinans, Along A Salinity Gradient, Danielle Neveu

USF Tampa Graduate Theses and Dissertations

Coastal communities will be most affected by global climate change and are important to study to understand current and future ecological processes. The current model for global climate change predicts a change in rainfall, which will alter the salinity of coastal systems. Given the presence of eutrophication in many coastal waters, it is important to understand the effects that this increase in nutrients, coupled with changes in salinity, will have on these communities. This study was conducted to understand the effect of salinity increase on the growth and herbivory of the black mangrove, Avicennia germinans, in the presence of …

Constructing A Healthcare Assets Map In Rural Appalachia: An Analysis Of Healthcare Services And Perceived Health Threats, Catherine Myers

USF Tampa Graduate Theses and Dissertations

Using data gathered over the course of two months through participant observation and semi-structured interviews with health providers (n=19) and community members (n=20), this research analyzes patient access to health care resources and describes community members' and health care providers' perceptions of pressing health concerns in their area. The results of this research show the types of health care resources in the county, the similarities and differences between health providers' and community members' perceptions, and how the unique characteristics of this community influence health care access and health disparity.

La Dictadura Desde La Escritura Femenina De Carmen Martín Gaite, Julia Álvarez E Isabel Allende, Mariella Orama

USF Tampa Graduate Theses and Dissertations

bold ABSTRACT

Understanding Transport Variability Of The Antarctic Circumpolar Current Using Ocean Bottom Pressure, Jessica Makowski

USF Tampa Graduate Theses and Dissertations

Previous studies have suggested that ocean bottom pressure (OBP) can be used to measure the transport variability of the Antarctic Circumpolar Current (ACC). The OBP observations from the Gravity Recovery and Climate Experiment (GRACE) are used to calculate transport along the choke point between Antarctica and Australia. Statistical analysis will be conducted to determine the uncertainty of the GRACE observations using a simulated data set.

There has been some evidence to suggest that Southern Hemisphere winds and the Southern Annular Mode (SAM) or the Antarctic Oscillation (AAO) play a significant role in accelerating/decelerating ACC transport, along with some contribution from …

Efficacy Of A Dissonance-Based Intervention For Self-Objectification: A Randomized Controlled Trial, Jessie Erin Menzel

USF Tampa Graduate Theses and Dissertations

Self-objectification is the process by which women take on a third-person perspective in evaluating their physical appearance and sexual attributes. Objectification theory states that self-objectification may lead to negative mental health outcomes in women; a growing body of cross-sectional and experimental research supports the connection between self-objectification and the experience of shame, disordered eating, depression, and sexual dysfunction. This study sought to evaluate an intervention designed to reduce self-objectification behaviors and beliefs in order to prevent the development of disordered eating, depression, and sexual dysfunction. An efficacious prevention program using cognitive dissonance induction was adapted to target self-objectification. The efficacy …

Persistent Infection With Human Herpesvirus-6 In Patients With An Inherited Form Of The Virus: A Newly Described Disease, Shara Pantry

USF Tampa Graduate Theses and Dissertations

Human Herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are ubiquitous betaherpesviruses. Both viruses are associated with a variety of adult disorders including neurological disorder, such as multiple sclerosis and chronic fatigue syndrome. HHV-6 viruses are capable of establishing latency by integration into the telomeres of the host chromosome and are transmitted in a Mendelian manner in approximately one percent of the population. To date little is known about the immunological and neurological consequences of HHV-6 inheritance. This study focused on a unique population of individuals that inherited HHV-6 and present with chronic fatigue-like symptoms, including hypersomnia, generalized fatigue, headache, and short …

Dead Man's Switch: Disaster Rhetorics In A Posthuman Age, Daniel Patrick Richards

USF Tampa Graduate Theses and Dissertations

When a disaster the magnitude of the Deepwater Horizon blowout and oil spill takes place, is it natural for the news media stories, investigative reports, and public deliberation to focus almost exclusively on finding the person or group responsible for such a horrendous scene. Rhetorically speaking, the discourse surrounding the event can be characterized as a reductive form of praise and blame rhetoric (epideixis). However, these efforts, while well-intentioned, are troublesome because searches for the one technical cause and the sole personal culpability are thwarted by the sheer complexity of the ecological, technological, scientific, institutional, and communicative network required for …

Control Of Smart Building Using Advanced Scada, Vivin Thomas Samuel

USF Tampa Graduate Theses and Dissertations

ABSTRACT

For complete control of the building, a proper SCADA implementation and the optimization strategy has to be build. For better communication and efficiency a proper channel between the Communication protocol and SCADA has to be designed.

This paper concentrate mainly between the communication protocol, and the SCADA implementation, for a better optimization and energy savings is derived to large scale industrial buildings. The communication channel used in order to completely control the building remotely from a distant place. For an efficient result we consider the temperature values and the power ratings of the equipment so that while controlling the …

Ecology Of The Late Neogene Extinctions: Perspectives From The Plio-Pleistocene Of Florida, Shubhabrata Paul

USF Tampa Graduate Theses and Dissertations

The ecological impact of past extinction events is one of the central issues in paleobiology. In face of present environmental changes, a better understanding of past extinctions will enable us to identify the magnitude of biodiversity crises and their underlying processes. The Late Neogene was a time of extraordinary climatic reorganization, including Northern Hemisphere glaciation, the rise of the Central American Isthmus, and associated changes in environmental conditions. Therefore, the Late Neogene extinctions of marine molluscs of South Florida present an ideal platform to examine the interaction between environmental changes and biotic response. In the present study, three different aspects …