Digital Commons Network^™

After Prometheus, Are Human Genes Patentable Subject Matter?, Douglas L. Rogers

Duke Law & Technology Review

On April 15, 2013, the U.S. Supreme Court heard oral arguments in Association for Molecular Pathology v. Myriad Genetics, Inc. on the question, “Are human genes patentable?” This article argues that human genes are not patentable and that isolating a gene from its surroundings in a human body—or creating synthetically what exists in nature as DNA—does not cause the DNA to become patentable subject matter. The isolated DNA segments of claim 1 have the identical nucleotide sequence and the same function as native DNA, and the isolated DNA of claim 1 do not reflect the marked changes required under Chakrabarty, …

Using Pcr To Target Misconceptions About Gene Expression, Leslie Kate Wright, Dina L. Newman

Articles

We present a PCR-based laboratory exercise that can be used with first- or second-year biology students to help overcome common misconceptions about gene expression. Biology students typically do not have a clear understanding of the difference between genes (DNA) and gene expression (mRNA/protein) and often believe that genes exist in an organism or cell only when they are expressed. This laboratory exercise allows students to carry out a PCR-based experiment designed to challenge their misunderstanding of the difference between genes and gene expression. Students first transform E. coli with an inducible GFP gene containing plasmid and observe induced and un-induced …

Awards Will Help A&S Students Pursue Research Projects, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Reaching Out To Faculty: Leveraging Professional And Social Interactions To Meet Information Needs, Valerie E. Perry

Library Presentations

Objectives: To apply the subject expertise of a life sciences librarian in serving the One Health information needs of the University of Kentucky College of Agriculture faculty and other researchers employing animal research protocols through professional involvement, education, and social activities.

Methods Setting: A large research university with diverse use of animal research across the disciplinary faculties. Population: Researchers in the University of Kentucky College of Agriculture and those in the medical, social, and life sciences--such as dentistry, medicine, pharmacy, biology and psychology--who use animals in their research. Exposure: Provide and promote information resources and services during 2010-2012 through a …

Early Influences On The Development Of Food Preferences, Alison K. Ventura, John Worobey

Kinesiology and Public Health

The ability to perceive flavors begins in utero with the development and early functioning of the gustatory and olfactory systems. Because both amniotic fluid and breast milk contain molecules derived from the mother’s diet, learning about flavors in foods begins in the womb and during early infancy. This early experience serves as the foundation for the continuing development of food preferences across the lifespan, and is shaped by the interplay of biological, social, and environmental factors. Shortly after birth, young infants show characteristic taste preferences: sweet and umami elicit positive responses; bitter and sour elicit negative responses. These taste preferences …

Application Carrier Volume: A Comprehensive Evaluation Of An Ultra-Low Volume Sprayer Compared To A Conventional Sprayer For Row-Crop And Turfgrass Production Systems, Jason Connor Ferguson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

An Ultra-Low Volume (ULV) sprayer was developed to decrease carrier volume required for pesticide applications in row and turfgrass cropping systems. The ULV sprayer can make spray applications at or below 19 L ha^-1, which is far lower than a conventional sprayer in row crop or turfgrass production systems. Field studies were conducted at the University of Nebraska West Central Research and Extension Center Dryland Farm near North Platte, NE and the John Seaton Anderson Turfgrass Research Facility near Mead, NE to compare the ULV sprayer to a conventional sprayer. Studies were conducted to compare the two sprayers …

Oral Transmission Of Listeria Monocytogenes In Mice Via Ingestion Of Contaminated Food, Elsa N. Bou Ghanem, Tanya Myers-Morales, Grant S. Jones, Sarah E. F. D'Orazio

Microbiology, Immunology, and Molecular Genetics Faculty Publications

L. monocytogenes are facultative intracellular bacterial pathogens that cause food borne infections in humans. Very little is known about the gastrointestinal phase of listeriosis due to the lack of a small animal model that closely mimics human disease. This paper describes a novel mouse model for oral transmission of L. monocytogenes. Using this model, mice fed L. monocytogenes-contaminated bread have a discrete phase of gastrointestinal infection, followed by varying degrees of systemic spread in susceptible (BALB/c/By/J) or resistant (C57BL/6) mouse strains. During the later stages of the infection, dissemination to the gall bladder and brain is observed. The …

Sociodemographic Risk Factors Of Glycemic Control For Youth With T1d: Cross-Sectional And Longitudinal Patterns Of Hba1c, Priscilla Powell

Theses and Dissertations

Individual growth curve (IGC) modeling evaluated longitudinal trajectories of glycemic control and diabetes care of youth with Type 1 Diabetes (T1D) over three years. IGC modeling allowed comparison of confounded sociodemographic predictors of disease outcomes that included ethnicity, SES, parent marital status, family structure, as well as disease duration, to determine the relative impact of these factors in the evolution of HbA1c and diabetes care throughout adolescence. At baseline, participants recruited from two pediatric endocrinology clinics included 198 youth, ages 9-15 (M age = 12.65, 77% Caucasian, 74% lived with married biological parents, M SES = 45.70) with average HbA1c …

Identification Of The Microbial Life On The Bonneville Salt Flats, Laura Wolf, Betsy Kleba

Utah Space Grant Consortium

No abstract provided.

Mercury Biogeochemistry In Great Salt Lake: Delineating The Microorganisms Involved In Methylation And Ecosystem Detoxification, Austin Wood, Bonnie Baxter

Utah Space Grant Consortium

No abstract provided.

All-Possible-Couplings Approach To Measuring Probabilistic Context., Ehtibar Dzhafarov, Janne V. Kujala

Department of Psychological Sciences Faculty Publications

From behavioral sciences to biology to quantum mechanics, one encounters situations where (i) a system outputs several random variables in response to several inputs, (ii) for each of these responses only some of the inputs may “directly” influence them, but (iii) other inputs provide a “context” for this response by influencing its probabilistic relations to other responses. These contextual influences are very different, say, in classical kinetic theory and in the entanglement paradigm of quantum mechanics, which are traditionally interpreted as representing different forms of physical determinism. One can mathematically construct systems with other types of contextuality, whether or not …

Development And Evaluation Of The Assessment Of Opioid Taking Behaviors And Adherence Scale (Aotba) In Patients With Sickle Cell Disease, Abdulkhaliq Jassem Alsalman

Theses and Dissertations

The rapid growth in opioid therapy for non-cancer pain has occurred without an adequate appreciation of the consequences of this growth. Few studies provide patient-centered evidence that can be used to inform the current proposed standards for efficacious (safe and effective) opioid prescribing in non-cancer pain. Furthermore, different terms may be used interchangeably in the literature to refer to opioid-taking behaviors, resulting in imprecise or vague interpretation of existing evidence. We therefore sought to explore patterns of opioid-taking behavior and their biopsychosocial-spiritual determinants in African-American adults with sickle cell disease (SCD). Many questions surround opioid use for non-cancer pain, but …

Elemental Concentrations In The Seed Of Mutants And Natural Variants Of Arabidopsis Thaliana Grown Under Varying Soil Conditions, Stephen C. Mcdowell, Garo Akmakjian, Chris Sladek, David Mendoza-Cozatl, Joe B. Morrissey, Nick Saini, Ron Mittler, Ivan Baxter, David E. Salt, John M. Ward, Julian I. Schroeder, Mary Lou Guerinot, Jeffrey F. Harper

Dartmouth Scholarship

The concentrations of mineral nutrients in seeds are critical to both the life cycle of plants as well as human nutrition. These concentrations are strongly influenced by soil conditions, as shown here by quantifying the concentration of 14 elements in seeds from Arabidopsis thaliana plants grown under four different soil conditions: standard, or modified with NaCl, heavy metals, or alkali. Each of the modified soils resulted in a unique change to the seed ionome (the mineral nutrient content of the seeds). To help identify the genetic networks regulating the seed ionome, changes in elemental concentrations were evaluated using mutants corresponding …

Predictors Of Agitation In The Critically Ill, Ruth Burk

Theses and Dissertations

BACKGROUND: Agitation is a common complication in the intensive care unit (ICU) manifested in behavior and actions that range from simple apprehension or anxiety to frankly combative behavior.5 Agitation is associated with significant adverse outcomes.1-3 Studies report up to 71% of ICU patients have some degree of agitation during their ICU stay and that agitation is observed 32% of the time.3;4 Potential causes of agitation in critically ill patients are numerous; however, data about factors that predict agitation are limited. OBJECTIVE: The specific aim of this study was to identify predictors of agitation on admission to the ICU as well …

Using Pcr To Target Misconceptions About Gene Expression, Leslie Kate Wright, Dina L. Newman

Articles

We present a PCR-based laboratory exercise that can be used with first- or second-year biology students to help overcome common misconceptions about gene expression. Biology students typically do not have a clear understanding of the difference between genes (DNA) and gene expression (mRNA/protein) and often believe that genes exist in an organism or cell only when they are expressed. This laboratory exercise allows students to carry out a PCR-based experiment designed to challenge their misunderstanding of the difference between genes and gene expression. Students first transform E. coli with an inducible GFP gene containing plasmid and observe induced and un-induced …

Underlying Conditions, Mariam Eqbal

Theses and Dissertations

The following is a collection of lines, curves, and dots on a plane, representing words fashioned to communicate my ideas about relationships between the thing and the nothing. This thesis is like a map, a contour drawing, an assortment of lines, speaking my thoughts about things in relation to space and time. As something moving, developing into another thing, as a thing sentient, I am viewing, performing, and processing incremental acts. I am looking at water and I can see my reflection break into a thousand pieces. It is like watching time.

Habitat And Host Indicate Lineage Identity In Colletotrichum Gloeosporioides S.L. From Wild And Agricultural Landscapes In North America, Vinson P. Doyle, Peter V. Oudemans, Stephen A. Rehner, Amy Litt

Publications and Research

Understanding the factors that drive the evolution of pathogenic fungi is central to revealing the mechanisms of virulence and host preference, as well as developing effective disease control measures. Prerequisite to these pursuits is the accurate delimitation of species boundaries. Colletotrichum gloeosporioides s.l. is a species complex of plant pathogens and endophytic fungi for which reliable species recognition has only recently become possible through a multi-locus phylogenetic approach. By adopting an intensive regional sampling strategy encompassing multiple hosts within and beyond agricultural zones associated with cranberry (Vaccinium macrocarpon Aiton), we have integrated North America strains of Colletotrichum gloeosporioides s.l. from …

Can Theories Of Animal Discrimination Explain Perceptual Learning In Humans?, Chris Mitchell, Geoffrey Hall

School of Psychology

We present a review of recent studies of perceptual learning conducted with nonhuman animals. The focus of this research has been to elucidate the mechanisms by which mere exposure to a pair of similar stimuli can increase the ease with which those stimuli are discriminated. These studies establish an important role for 2 mechanisms, one involving inhibitory associations between the unique features of the stimuli, the other involving a long-term habituation process that enhances the relative salience of these features. We then examine recent work investigating equivalent perceptual learning procedures with human participants. Our aim is to determine the extent …

Female Paramilitary Experience In Northern Ireland, Emily Artalejo

Honors Program Theses and Research Projects

"Peace is not what our people fought for. They fought for independence." Declared Bernadette Sands-McKevitt in response to the Provisional Irish Republican Army's acceptance of the Belfast Agreement. A former member of the PIRA, Sands-McKevitt is now an active member of the "Real IRA," a splinter group formed by republicans who were disillusioned by Gerry Adams' acceptance of the Mitchell Principles during the Northern Ireland Peace Process. She is the founder and current Vice Chair Person of the 32-County Sovereignty Committee, a single-issue pressure group designed to eliminate the partitionist articles of the Agreement and unite Northern Ireland with the …

Commencement Convocation Program, St. Petersburg Campus, May 5, 2013, University Of South Florida

USF Graduation and Convocation Programs

King O'Neal Scholar - Sean D. Ericson; Outstanding Graduate - Aaron G. Kawaja

Commencement Convocation Program, Sarasota - Manatee Campus, May 5, 2013, University Of South Florida

USF Graduation and Convocation Programs

Outstanding Professor Award - Richard Reich; Golden Bull Service & Outstanding Leadership Award - Ryan Page; Outstanding Graduate - Dat Vu; King O'Neal Scholar - Michaela Cerce

Perception, Expression And Management Of The Pain Of Childbirth, Mary H. Christenson

Senior Honors Theses

In order to be a competent nurse on an American labor and delivery unit it is important to have an understanding of the pain of childbirth. This includes a thorough understanding of pain as a sensation and its manifestation during the birthing experience. An understanding of pain is useless, however, unless standardized pain assessment practices are used. The most accurate pain assessment is associated with a general understanding of cultural trends in pain perception and expression. Along with culture, other factors also influence how a woman senses and copes with the pain of labor. Anxiety is one of these influential …

A Pilot Test On The Role Of Power In Mate Choice, Addison Zhao

Honors Scholar Theses

To test two competing theories, social role and sexual strategies, a study was proposed to have participants evaluate dating profiles that varied in agency, communion, and status. Power was also manipulated to test for effects on likelihood to date an individual. To test methods used for the proposed study, a pilot study was conducted to evaluate the effectiveness of a power prime task, a manipulation check for the power prime task, the attractiveness of 24 pictures, and an analysis of 12 profiles. The study found that power priming with analogies did not significantly lead to participants feeling powerful. This finding …

Molecular Characterization Of A Patient Presumed To Have Prader-Willi Syndrome, Marina Falaleeva, Carlos R. Sulsona, Horst R. Zielke, Kathleen M. Currey, Pierre De La Grange, Vahid Aslanzadeh, Daniel J. Driscoll, Stefan Stamm

Molecular and Cellular Biochemistry Faculty Publications

Prader-Willi syndrome (PWS) is caused by the loss of RNA expression from an imprinted region on chromosome 15 that includes SNRPN, SNORD115, and SNORD116. Currently, there are no mouse models that faithfully reflect the human phenotype and investigations rely on human post-mortem material. During molecular characterization of tissue deposited in a public brain bank from a patient diagnosed with Prader-Willi syndrome, we found RNA expression from SNRPN, SNORD115, and SNORD116 which does not support a genetic diagnosis of Prader-Willi syndrome. The patient was a female, Caucasian nursing home resident with history of morbid obesity (BMI 56.3) and mental retardation. She …

Relating Air Quality In The Work Environment To Occupational Health Disease, Hussien Awad Alumar

Doctoral Dissertations and Master's Theses

Comprehensive knowledge of the air pollutants effects on the human health generally is a crucial requirement in developing effective policies for reducing such adverse effects related to ambient air pollution. Such knowledge would be essential in helping various affected countries, including the less developed countries (LDCs), to develop effective regulatory frameworks for assessing and managing air quality in the workplaces. Developed and developing countries therefore need to develop effective techniques or strategies focused towards improving the workplace monitoring competence in diverse industrial environments. Such initiatives are essential in reducing or eliminating the industrial pollutants within the workplaces while also helping …

Illinois Wesleyan Celebrates Class Of 2013 At Commencement, Kim Hill

News and Events (Discontinued Series)

No abstract provided.

Commencement 2013 (Program And Video), Illinois Wesleyan University

Commencement Ceremonies

No abstract provided.

Finding History In The Future, Al-Sowaidi Aisha

Theses and Dissertations

Change and development over an extremely fast period of time in Qatar have shifted the atmospheric sense of the country. The distance created by the skyscrapers and their scale to people has a great impact on the behavior and interaction between the people and the city. In my research, I aim to incorporate the old experiences and behaviors with contemporary design in objects used within the house to maintain the feeling of being home through reliving the fading behaviors and traditions as well as bringing closer the modern city into the home through the use of materials. Through experimentation with …

Celebration Schedule 2013 (Saturday), Provost's Office

Celebration

Full presentation schedule for Celebration, Saturday, May 4, 2013

Sea Turtle Protection In The Eastern Equatorial Pacific: A Strategy To Reduce Incidental Take And Promote Conservation, Brittany Bartlett

Open Access Theses

Five species of sea turtle inhabit the Eastern Tropical Pacific Seascape. Based on the International Union for the Conservation of Nature, all five of these populations are threatened with extinction. Bycatch, the incidental take of non-target species in fisheries, is the leading cause of sea turtle decline within the region. To address this issue in the region, methods were used to analyze policy and management in attempts to better determine those gaps, which hinder sea turtle protection. Geographic Information Systems, specifically, ArcGIS, was used determine areas of primary importance to sea turtles. The Inter American Tropical Tuna Commission (IATTC) provided …