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The Science (And Pseudoscience) Of Dna Profiling, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Nf1 Loss And Ras Hyperactivation In Oligodendrocytes Induce Nos-Driven Defects In Myelin And Vasculature, Debra A. Mayes, Tilat A. Rizvi, Haley E. Titus-Mitchell, Rachel Oberst, Georgianne M. Ciraolo, Charles V. Vorhees, Andrew P. Robinson, Stephen D. Miller, Jose A. Cancelas, Anat O. Stemmer-Rachamimov, Nancy Ratner

Neuroscience, Cell Biology & Physiology Faculty Publications

Patients with neurofibromatosis type 1 (NF1) and Costello syndrome Rasopathy have behavioral deficits. In NF1 patients, these may correlate with white matter enlargement and aberrant myelin. To model these features, we induced Nf1 loss or HRas hyperactivation in mouse oligodendrocytes. Enlarged brain white matter tracts correlated with myelin decompaction, downregulation of claudin-11, and mislocalization of connexin-32. Surprisingly, non-cell-autonomous defects in perivascular astrocytes and the blood-brain barrier (BBB) developed, implicating a soluble mediator. Nitric oxide (NO) can disrupt tight junctions and gap junctions, and NO and NO synthases (NOS1-NOS3) were upregulated in mutant white matter. Treating mice with the NOS inhibitor …

Impact Of Postpartum Anxiety And Depression On Child’S Mental Development From Two Peri-Urban Communities Of Karachi, Pakistan: A Quasi-Experimental Study, Niloufer Sultan Ali, Sadia Mahmud, Asia Khan, Badar Sabir Ali

Department of Family Medicine

Background

Postpartum anxiety and depression has detrimental effects on the overall mental development of children. This study aims to assess the impact of postpartum anxiety and depression on children’s mental development on all sub-scales in a Pakistani population.

Methods

A quasi-experimental study was conducted in two peri-urban communities of Karachi, a mega city of Pakistan, to assess the impact of postpartum anxiety and depression on children’s growth and mental development. A total of 420 women were enrolled, who had given consent out of 651 pregnant women identified, during February 2004 to December 2005. Data for socio-demographic, home environment and family …

Finding Common Feminist Ground: The Role Of The Next Generation In Shaping Feminist Legal Theory, Kathleen Kelly Janus

Duke Journal of Gender Law & Policy

This article explores the ways in which current feminist frameworks are dividing the women’s movement along generational lines, thereby inhibiting progress in the struggle for gender equality. Third-wave feminists, or the generation of feminists that came of age in the 1990s and continues today, have been criticized for focusing on personal stories of oppression and failing to influence feminist legal theory. Yet this critique presupposes that third-wave feminism is fundamentally different from the feminism of past generations. In contrast, this article argues that third-wave feminism is rooted in the feminist legal theory developed in the prior generation.

This article demonstrates …

A Simple And Low-Cost Technique Of Dna Extraction From Edible Mushrooms Examined By Molecular Phylogenetics, F.A. Avin, S. Bhassu, V. Sabaratnam

Research Publications (2011 to 2015)

The first and most important step of molecular techniques is to isolate the high quality and standard quantity of DNA. The DNA extracted using the recommended method could successfully amplify the regions of interest and demonstrated reliable results can be applied in other molecular assays. Moreover, the designed primers of ITS1-UM2 and ITS4-UM2 were perfectly matched with the species of Basidiomycetes, can be used in phylogenetic studies of other mushrooms. We here evaluated the quality and quantity of DNA using a spectrophotometer, showed reliable OD260/280, and concentration. The protocol is efficient, rapid, low-cost, and simple, needs low amount of sample, …

Streptomyces Strain P42 As A Potent Biological Control Against Chilli Anthracnose Disease Caused By Colletotrichum Spp., P. Shahbazi, M.Y. Musa, G.Y.A. Tan, F.A. Avin, W.F.A. Teo, S. Vikineswary

Research Publications (2011 to 2015)

A total of 130 actinomycetes was isolated from rhizospheres of healthy and diseased (Colletotrichum-infected) chilli plants and tested for their inhibitory activity against Colletotrichum acutatum, Colletotrichum gloeosporioides and Colletotrichum truncatum. Twenty-six isolates were active against at least one of the Colletotrichum species and 12 were active against all three anthracnose fungi. Four bioactive isolates, P8, P39, P42 and P115, that exerted high inhibitory activity against all three anthracnose fungi species, were identified using molecular methods. Phylogenetic analysis of the 16S rRNA gene sequences revealed that all four isolates belonged to the genus Streptomyces. Strain P42, which showed the highest inhibitory …

The Diagnostic Performance Of A Single Genexpert Mtb/Rif Assay In An Intensified Tuberculosis Case Finding Survey Among Hiv-Infected Prisoners In Malaysia, H.A.A. Al-Darraji, H.A. Razak, K.P. Ng, F.L. Altice, A. Kamarulzaman

Research Publications (2011 to 2015)

The new GeneXpert MTB/RIF assay (Xpert) offers accurate and rapid diagnosis of active TB, but its performance in improving case detection in high-transmission congregate settings has yet to be evaluated. We assessed the diagnostic accuracy of a single Xpert assay in an intensified case finding survey among HIV-infected prisoners in Malaysia. Methods: HIV-infected prisoners at a single site provided two early-morning sputum specimens to be examined using fluorescence smear microscopy, BACTEC MGIT 960 liquid culture and a single Xpert. The sensitivity,specificity, negative and positive predictive values of Xpert were calculated relative to gold-standard results using MGIT 960 liquid culture. Relevant …

Federal Education Laws And The Fine Arts, Kim R. Cavener

Dissertations

Due to federal laws requiring standardized testing of only a select few of the core subjects, many students have been divested of fine arts instruction (Chen, 2008; Garcia, 2010; Jacobsen & Rothstein, 2009; Maxwell, 2008; Suzuki, 2009). Moreover, school officials have reduced funding allocated to non-tested content areas as one means of balancing district budgets in a poor economy (Chen, 2008; Garcia, 2010). This mixed method study examined music educators’ and curriculum directors’ perceptions of how federal education laws have affected public school fine arts. Analysis of data from interviews of six music educators and six curriculum directors were conducted …

Unconscious Priming Requires Early Visual Cortex At Specific Temporal Phases Of Processing, Marjan Persuh, Tony Ro

Publications and Research

Although examples of unconscious shape priming have been well documented, whether such priming requires early visual cortex (V1/V2) has not been established. In the current study, we used TMS of V1/V2 at varying temporal intervals to suppress the visibility of preceding shape primes while the interval between primes and targets was kept constant. Our results show that, although conscious perception requires V1/V2, unconscious priming can occur without V1/V2 at an intermediate temporal interval but not at early (5–25 msec) or later (65–125 msec) stages of processing. Because the later time window of unconscious priming suppression has been proposed to interfere …

Automated Methods To Determine Electrospun Fiber Alignment And Diameter Using The Radon Transform, Nicholas J. Schaub, Sean J. Kirkpatrick, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

Recent evidence demonstrates the ability to change cell function by altering the physical properties of electro spun scaffolds, but many studies still do not characterize electro spun fiber alignment and diameter. To aid in the reporting of these crucial properties, we demonstrate two methods of quantifying electro spun fiber alignment with one method capable of determining electro spun fiber diameter. The first method assesses fiber alignment in a scanning electron microscopy image using the Radon Transform (RT) to calculate the entropy of the fibers in the image. The RT entropy method was more sensitive than a commonly used Fast Fourier …

Clinical And Dosimetric Implications Of Air Gaps Between Bolus And Skin Surface During Radiation Therapy, Yousaf Khan, J Eduardo Villarreal-Barajas, Mona Udowicz, Richie Sinha, Wazir Muhammad, Ahmed N. Abbasi, Amjad Hussain

Department of Radiation Oncology

Purpose: The main objective of the study was to evaluate the effect of air gaps of 0 - 5.0 cm between bolus and skin for 1.0 cm Superflab bolus on surface dose (DSurf) and depth of maximum dose (dmax) in solid water and Rando® phantoms.

Methods: In this work, the effects of bolus to surface distance on DSurf and variation in dmax were analyzed in a solid water phantom and in an anthropomorphic Rando® phantom for different field sizes, using Gafchromic® EBT films and farmer chamber.

Results: For field sizes of 5 × 5 cm2 the DSurf …

Au Revoir, Will Contests: Comparative Lessons For Preventing Will Contests , Margaret Ryznar, Angelique Devaux

Nevada Law Journal

No abstract provided.

Testifying Minors: Pre-Trial Strategies To Reduce Anxiety In Child Witnesses, Dawn Hathaway Thoman

Nevada Law Journal

No abstract provided.

Wanted: Forever Home – Achieving Permanent Outcomes For Nevada's Foster Children, Miriam C. Meyer-Thompson

Nevada Law Journal

No abstract provided.

Synthesis, Structural Studies Of Some New Transition Metals Complexes Of Semicarbazide Hydro Chloride Schiff Base Derivatives, Abbas Asalih Al-Hamdani, Muhannd A Mahmoud, Shaimaa.R Bakir

Baghdad Science Journal

A new series of transition metal complexes of Cu(II), Ni(II), Co(II) and Fe(III) have been synthesized from the Schiff base (L1) and (L2) derived from Semicarbazide hydro chloride and 4-chlorobenzaldehyde or 4-bromobenzaldehyde. The structural features have been arrived from their elemental analyses, magnetic susceptibility, molar conductivity, IR, UV-Vis. and 1H NMR spectral studies. The data show that the complexes have composition of [M(L)2](NO3)2 and [Fe(L)2 (NO3)2](NO3) where the M=Co(II),Ni(II) and Cu(II) ;L=L1and L2 type. The magnetic susceptibility and UV-Vis spectral data of the complexes suggest a square planer geometry for Co(II) and Cu(II) but Fe(III) octahedral geometry and Ni(II) tetrahedral …

Spectroscopic Characterization And Antimicrobial Activities Of Some Heavy Metals Complexes Of 2-Hydroxy Phenyl Piperonalidene, Mahasin Faisal Alias, Israa Qais Yousif, Sura Kalil Ibrahim

Baghdad Science Journal

An antibacterial and antifungal piperonal-derived compound and its Rh(III), Pd(II), Pt(IV), and Cd(II) metal complexes were synthesized and characterized by spectroscopic methods, conductivity, metal analyses and magnetic moment measurements. The nature of the complexes formed in ethanolic solution was studied following the molar ratio method. From the spectral studies, octahedral geometry was suggested for rhodium (III) and platinum (IV) complexes, while a square planer structure was suggested for palladium (II) complex and a tetrahedral geometry for cadmium (II) complex. Structural geometries of these compounds were also suggested in gas phase by using hyperchem-8 program for the molecular mechanics and semi-empirical …

Synthesis, Spectral Studies, And Theoretical Treatment Of New Ni(Ii),And Co(Ii) Complexes Of Bidenetate Ligands 2-Benzamido Benzothiazole ,And 2-Actamido Benzothiazole, Mahasin F. Alias, Ameena N. Seewan

Baghdad Science Journal

New metal complexes of the ligands 2-benzamido benzothiazole(B1), and 2-actamido benzothiazole(B2) with metal ions Ni(II),and Co(II) were prepared in alcoholic medium. The prepared complexes were characterized by FT-IR and electronic spectroscopy, Magnetic susceptibility, Flame Atomic Absorption technique as well as elemental analysis and conductivity measurement.Fromthe spectral studies, an octahedral monomer structure proposed for Ni(II) complexes, and a tetrahedral monomer structure for Co(II)complexes.Semi-empirical methods (PM3,and ZINDO/1)were carried out to evaluate the heat formation( ∆H ̊f)binding energy(∆Eb) and dipole moment(μ)for all metal complexes. Also vibration frequencies, Electrostatic potential, HOMO and LUMO energies for ligands were calculated.

Synthesis And Characterization Of New Complexes Of Co+2 , Ni+2 , Cu+2 And Zn+2 With (Sodium Acetate Thio ) Ethylene Ligands, N.H Buttrus, H.A Mohammed

Baghdad Science Journal

This research involves the preparation of new ligands 1,1,2,2- tetrakis (sodium acetate thio)ethylene(L1) and 1,1,2- tris(sodiumacetatethio) ethylene(L2), through the reaction of disodium thioglycolate) with tetra chloro ethylene or tri chloro ethylene in (1:4) or (1:3) moler ratio . Homodinucliar complexes of general formlu [M2(L1)] and [M2(L2)ClH2O] , when M= Co(II), Ni(II), Cu (II) and Zn(II) also mono nuclear complexes of general formula [M(L2)] . The prepared complexes were characterized using spectral method (UV/Visible/ IR) , metal content analysis , magnetic and atomic measurements . The spectral and magnetic measurement indicats that some complexes have tetrahedral or square planar complexes environtment …

Synthesis Of N-Benzothiazole Derivative Imide On Polymeric Chain, Have Possible Biological Activity, Entesar O Al-Tamimi, Mohmoud A-Al-Issa, Hind F. Al-Gburi

Baghdad Science Journal

In this reserch Some new substituted and unsubstituted poly imides compounds. were synthesized by reaction of acrylol chloride with different amides (aliphatic and aromatic) in a suitable solvent in the presence amount triethyl amine (Et3N) with heating. The Structure confirmation of all polymers were confirmed using FT-IR,1H-NMR,13C-NMR and UV spectroscopy. Thermal analysis (TG) for some polymers showed their thermal stabilities. Other physical properties including softening points, melting point and solubility of the polymers were also measured

Synthesis And Characterization Of New Thioxanthone Derivatives, Souad. M. Al-Araji, Rafid. S. Dawood

Baghdad Science Journal

This work comprises the synthesis of new thioxanthone derivatives containing C-substituted thioxanthone. To obtain these derivatives, the o-mercapto benzoic acid was chosen as the starting material, which was reacted with dry benzene in sulfuric acid (98 %) to produce the thioxanthone (1). The 2,7-(disulfonyl phosphine imine) thioxanthone (4-8) were prepared from reaction of compound (1) with chlorosulfonic acid gave 2,7-(disulfonyl chloride) thioxanthone (2). Treatment of (2) with sodium azide to produce 2,7-(disulfonyl azide) thioxanthone (3). Condensation of (3) with phosphorus compounds afforded compounds (4-8). The 2,7-(disulfonamide) thioxanthone (9-21) was obtained when compound (2) condensed with different aromatic amines, it gave …

Synthesis And Biological Studies For Some Heterocyclic Compounds Derived From 2-Morpholino-1,8- Naphthyridine-4-Carboxylic Acid, Ala I. Ayoob

Baghdad Science Journal

New heterocyclic compounds derived from 2-Morpholino-1,8-naphthyridine-4-carboxylic acid such as oxadiazolo, thiadiazolo – thione and triazolo-thione have been prepared and characterized on the basis of IR and 1H NMR spectra data. The hydrizide compound was utilized as a starting material for preparing of these compounds. The second part of this study involves the biological studies of some of these naphthyridine derivatives by using three different kinds of bacteria namely: Staphylococcus aureus, Pseudomonas aeruglnosa and Escherichia coli. The data indicated that some of these compounds have a good activity against the tested bacteria in comparison to antibiotics.

Synthesis Of Some Heterocyclic Compounds Derived From 2-Mercapto Benzoxazole, Firyal W Askar, Huda A. Hassan, Nahida A Jinzeel

Baghdad Science Journal

New series of 2-mecapto benzoxazole derivatives (1-20) incorporated into fused to different nitrogen and suphur containing heterocyclic were prepared from 2-meracpto benzoxazole, when treated with hydrazine hydrate to afford 2-hydrazino benzoxazol (1). Compound (1) converted to a variety of pyridazinone andphthalazinone derivatives (2-4) by reaction with different carboxylic anhydride. Also, reaction of (1) with phenyl isothiocyanate and ethyl chloro acetate afforded 3-phenyl-1,3-thiazolidin-2,4-dione-2-(benzoxazole-2-yl-hydrazone) (6). Azomethines (7-10) were prepared through reaction of (1) with aromatic aldehyde, then (7, 8) converted to thaizolidinone derivatives (11, 12). Treatment of (1) with active methylene compounds afforded derivative (13). Reaction of (1) with CS2 and NaOH …

Induction Of Antioxidant Enzymes In Wheat (Triticum Spp.) Grown Under Salt Stress, Ismail K. Al-Samerria, Saadi M. Al-Ghrairi, Hamad Allah S. Rahi

Baghdad Science Journal

A pot culture experiment was conducted at the greenhouse of soil and water resources department in College of Agriculture, University of Baghdad in Abo-Ghraib at season 2009-2010 to investigate the effects of using foliar application of some macro and micronutrients in induce antioxidant enzymes in wheat grown under salt stress . Doar85 planted under three levels of salt stress, and three combinations of foliar application were used from nutrients (K+ Ca) at 3000 and 1500 mg.L-1 respectively, and (Fe + Zn + Mn) at 30, 20, and 10 mg.L-1 respectively , and ( K+ Ca) + (Fe+ Zn + Mn). …

Purification And Characterization Of Endoglucanase From Local Isolate Of Aspergillus Flavus, Essam F. Al-Jumaily, Fayhaa Muqdad Khaleel

Baghdad Science Journal

Endoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chromatography (Sepharose -6B), when a value of specific activity was 400 U/ mg protein, with a yield of 48 %. Certain properties of this purified enzyme were investigated, the optimum pH of activity was 7 and the pH of its stability was 4.5, while the temperature stability was 40 °C for 60 min. The enzyme retained …

Antioxidant And Free Radical Scavenging Effects Of Iraqi Sumac ( Rhus Coriaria L), Khadejah Y. Al-Muwaly, Khawola A. Al-Flayeh, Asmaaa. Ali

Baghdad Science Journal

This study was carried out to evaluate the antioxidant activity of Iraqi sumac seeds (Rhus coriaria. L) (Anacardiaceae). Total phenolic compounds and flavoniods were determined in three different sumac seed extracts (SSE) (aqueous,ethanolic and methanolic extract). For extraction Antioxidant activity of SSE were evaluated by various antioxidant assays, including total antioxidant capacity, reducing power,by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, nitric oxide scavenging, Hydroxyl radical scavenging, and metal ion chelating activities. These various antioxidant activities were compared with ascorbic acid as a standard antioxidant.The results showed that the three(SSE), contained large amounts of phenolic and flavoniods compounds. The results also …

In Vitro Study Of Mefenamatestarch As Drug Delivery System, Firyal M. Ali, Firas A. Rahi, Aseel Q. Q. M, Huda M. A

Baghdad Science Journal

Mefenamic acid was esterified with starchwith[1:1] Molar ratio, as drug substituted with natural polymer, to prolongthe period of hydrolysis of drug polymer with other advantages. The new prodrug starch was characterized by FT-IR and UV-Visible and 1H-NMR spectroscopies. The physical properties were studied and controlled drug release was studied in different pH values at 37oC. The stability of drug was carried out by measuring the absorbance of mefenamic starch which hydrolyzed in HCl solution of pH 1.1 (artificial gastric fluid) and phosphate buffer of pH 7.4 (simulating intestinal fluid SIF) at 37oC for several days. The thermal analysis such as …

Synthesis ,Spectroscopic Study Of Antipyryl Azo 2-Naphthol And Use It As New Reagent For Determination Of Co(Ii) And Cu(Ii), Hussain.J. Mohammed, Azhar.Y. Muhi, Fatema. Abbas

Baghdad Science Journal

A simple ,accurate and sensitive spectrophotometric method has been developed the determination of Cobalt(II) and Cupper (II) .The method is based on the chelation of Co(II) and Cu(II) ions with 4-(4´-pyrazolon azo) -2-Naphthol(APAN) in aqueous medium . The complexes have a maximum absorption at (513) and (506) nm and ɛ max 0.531×10 4 and 0.12×10 5 L.mol -1.cm -1 for Co(II) and Cu(II) respectively .The reagent and two complexes have been prepared in ethanolic solution.The stoichiometry of both complexes were found to be 1:2 (metal :legend) .The effects of various cations and anions on Co(II) and Cu(II) determination have been …

Cmc Determination And Thermodynamic Micellisation Of Npe Surfactant In Aqueous And Ch3oh – H2o Solvents, Sameer H. Kareem, Lamees Abid Zead

Baghdad Science Journal

The critical micelle concentration (CMC) of nonylphenolethoxylate (NPE) surfactant has been determined by measuring the surface tension as a function of the molar concentration of the surfactant in aqueous and binary mixture of water + methanol solutions at a temperature range from 20°C to 35°C. The interfacial parameters Γmax, Amin, Πcmc and ΔG ̊ads were calculated. The results indicate that the CMC increases as the temperature increases and that the addition of methanol the CMC decreases. The thermodynamic parameters such as standard Gibbs free energy (ΔG°), enthalpy (ΔH ̊), and entropy (ΔS ̊) of micellization were estimated using the change …

Kinetic Study Of The Hydrolysis Of Synthesized Ibuprofen Ester And Its Biological Activity, Noha Mohammad Yahya, Sabah G. Al-Dabbagh

Baghdad Science Journal

It is known that the oral administration of ibuprofen caused an irritation of stomach as a side effect due to its carboxylic moiety. Ibuprofen ester was synthesized by linking the carboxylic moiety of ibuprofen and the hydroxylic group of paracetamol to reduce its side effect. Study the kinetic hydrolysis of prepared ester was examined at different values of physiological pH (1.0, 5.8, 6.4 and 7.4) at 37 ± 0.1 of 1 hour period. Measurements of absorbance were carried out by UV-Visible spectrophotometer to follow the stability of ester, it showed Pseudo first order hydrolysis. The pH- apparent rate profiles of …

Strategic Landpower In The Indo-Asia-Pacific, John R. Deni

The US Army War College Quarterly: Parameters

No abstract provided.