Digital Commons Network^™

Developing A Multistage Model For Treponemal Disease Susceptibility, Sarah A (Sarah Ann) Mathena

Theses and Dissertations

Despite numerous studies, little is known about the influential factors impacting treponemal disease susceptibility. Yet, forms of treponemal disease, specifically yaws, are a major source of morbidity in the developing world. Bioarchaeological materials may assist with deficit by testing full ranges of disease expression. This study explores the relationship between treponemal disease ecology, specifically yaws, and susceptibility through six southeastern United States archaeological samples. Results show early life health experiences have a relationship to later life disease expression. However, influences of climate may only be seen at a cross-regional level. Finally, although nutrition, as evidenced by subsistence strategy, may play …

Understanding Binding-Induced Conformational Change In The Pin1 Prolyl Isomerase, Hawa Gyamfi

Theses and Dissertations

Pin1 is a Prolyl Isomerase that catalyzes cis-trans isomerization of peptides with pSer/Thr-Pro motifs in many cell signaling proteins. This conformational switch is implicated in diseases. Pin1 activity is considered a target for therapeutic applications. Pin1 targets motifs by its N-terminal WW-binding domain. A C-terminal PPIase domain is responsible for catalysis. To understand how Pin1 coordinates its enzymatic activities, it is necessary to probe how the domains behave in the presence of substrates. Here, we used novel (Histone H1 and Sic1) and other existing peptides to characterize the dynamics of Pin1 and impact of substrate binding on inter-domain interactions. Pin1- …

Urban Flow-Through Facilities' Media Compositions For Stormwater Quality And Quantity Improvements, Emily Gwynne Overbey

Theses and Dissertations

Urban stormwater management is evolving toward sustainable approaches which rely on dispersed small-scale bioretention BMPs. One such BMP is the flow-through planter, commonly applied in areas where infiltration into in situ soil is restricted or not possible. A project was developed to evaluate 18, vertically scaled flow-through mesocosms. Three replicates of six treatments, including four soil mixtures containing varied percentages of sand, compost and topsoil, were tested for orthophosphate and nitrate removal, volume reduction capabilities, and peak flow attenuation through the application of a synthetic solution over a simulated 2-inch, Type II storm event. Runoff volume was significantly (p < 0.05) reduced compared to controls. Nutrient levels observed along the hydrograph at different time-steps and flow rates revealed patterns not apparent in cumulative results. The observation of preferential flow patterns along with variability in nutrient removal across treatments highlights the need for design modifications of flow-through facilities.

Waterbird And Seed Abundances In Migratory Bird Habitat Initiative And Non-Managed Wetlands In Mississippi And Louisiana, Matthew Moraco Weegman

Theses and Dissertations

The USDA Natural Resources Conservation Service (NRCS) implemented the Migratory Bird Habitat Initiative (MBHI) in summer 2010 to provide wetlands for waterbirds inland from the Deepwater Horizon Oil Spill. To evaluate MBHI and associated wetland management practices, I estimated seed and waterbird densities in MBHI and non-managed wetlands in the Mississippi Alluvial Valley (MAV) of Mississippi and Louisiana. Although not statistically different, wetlands enrolled in MBHI contained 1.26 and 1.53 times more seed biomass and seeds consumed by waterfowl than non-managed wetlands, respectively. I also detected 3 times more dabbling ducks and all ducks combined on MBHI wetlands. When I …

Synthesis, Characterization, And Catalytic Activity Of Silica Supported Bimetallic Copper Catalysts For Organic Oxidation Reactions And The Study Of Benzylation Of Triketones, Nuwan Dileepa De Silva

Theses and Dissertations

The dissertation describes research work on development of bimetallic heterogeneous catalysts for oxidation of organic compounds. Binuclear metal complexes are an interesting class of compounds due to their catalytic activity. The approach involves anchoring a triketone ligand to Cab-O-Sil via a linker. Specifically, silica gel was allowed to react with p-chloromethylphenyltrimethoxysilane followed by coupling with deprotonated triketone compounds. The viability of this approach was verified by performing the benzylation reaction of triketones with benzyl halides under basic conditions. The benzylation of 2,4,6-heptanetrione and 1,5-diphenyl-1,3,5-pentanetrione is achieved with benzyl bromide using n-tetrabutylammonium fluoride as base. These benzylation reaction conditions were used …

Association Between Stink Bug Damage And The Incidence Of Phomopsis Longicolla In Mississippi Soybean Production, Joshua Lunn Jones

Theses and Dissertations

Stink bugs (Hemiptera: Pentatomidae) are key pests of soybean, Glycine max (L.), in Mississippi. Historically, yield loss derived from direct feeding by stink bugs has been considered the greatest threat to producers. However, quality reductions resulting from seed infections caused by microorganisms including Phomopsis longicolla are also a concern. Experiments were conducted in 2010 and 2011 to determine if stink bugs are associated with the incidence of P. longicolla in Mississippi soybean production. Data from experiments suggest that stink bugs are capable of transporting P. longicolla between two points. Data further suggest stink bugs and P. longicolla have the potential …

Analysis Of Humeral And Femoral Cross-Sectional Properties At Morton Shell Mound (16ib3), Sarah Marie Zaleski

Theses and Dissertations

Using the concept of bone functional adaptation, this study analyzes femoral and humeral cross-sectional properties of human skeletal materials from Morton Shell Mound on the Louisiana coast. This work helps fill a gap in such analyses in the southern U.S. and contributes to an understanding of the functional adaptation of the human skeleton. Properties were compared to those of other prehistoric Southeastern fisher-hunter-gatherers from Gold Mine, Plash Island, and several Georgia coast sites to assess mobility and activity patterns among inland and coastal groups. Less sexual dimorphism of femoral midshaft shape among coastal Morton and Plash, compared to inland Gold …

Bioluminescence Imaging Of Transgene Expression In Intact Porcine Ovarian Follicles In Vitro, Song-Yi Jung

Theses and Dissertations

The porcine antral follicle, which consists of an oocyte and surrounding follicular components, including theca, granulosa, and cumulus cells and follicular fluid, is an essential microenvironment for oocyte development and maturation. Investigating cellular and molecular events in the context of the whole follicle will aid in our understanding of interactions between the oocyte and the follicular components. The objective of this dissertation was to develop a novel bioluminescent imaging model to visualize and measure cellular and molecular events in living intact ovarian follicles in vitro. Bioluminescence imaging was employed to facilitate noninvasive, dynamic, and real-time transgene analysis in living intact …

Characterization And Monitoring Of On-Farm Water Storage Systems In Porter Bayou Watershed, Mississippi, Richard Ludwig Kirmeyer

Theses and Dissertations

The onarm water storage (OFWS) systems at Metcalf and Pitts farm in Porter Bayou Watershed were monitored for changes in nutrient levels and water withdrawal for irrigation from March 2012 to April 2013. Nitrogen and phosphorus levels were generally higher during the early part of the growing season. The OFWS systems can reduce nitrate and phosphorus in runoff up to 81% and 85%, respectively. However, the systems did not consistently reduce sediment and nutrients especially after significant rainfall and runoff events. The systems provided a total of 130 and 233 acreeet of recycled water for irrigating crops at Metcalf and …

Lightweight Moral Realism: Objectivity And Reasoning Without Heavyweight Facts, Mark Douglas Warren

Open Access Dissertations

In this dissertation, I develop a non-representational approach to metaethics that avoids the ontological and epistemic problems that traditional realist views face: accounting for the nature of moral facts and our knowledge of these facts in a way that comports with naturalism. According to expressivism—the most popular non-representational account in contemporary metaethics—moral claims don’t aim to represent moral facts or properties, but instead function to express our non-cognitive attitudes like approval or disapproval. This is a promising approach, but the view has substantial difficulties accounting for important features of moral discourse, like objectivity and complicated moral reasoning. I develop an …

A Study Of Internal Solitary Waves In Satellite Images Of The Mid-Atlantic Bight, Jingshuang Xue

Open Access Dissertations

Satellite imagery has been widely used to study internal solitary wave (ISW) generation mechanisms, evolution, and properties. The Nonlinear Internal Wave Initiative/Shallow Water 2006 (SW06) experiment in the Mid-Atlantic Bight (MAB) provided a unique dataset to study the vertical structure from their surface manifestation in the images, which can improve our understanding of ISW evolution, energy dissipation, and mixing processes. This thesis has two parts. First, the applicability of various weakly nonlinear theories including the Korteweg-de Vries (KdV), Intermediate Long Wave (ILW), Benjamin-Ono (BO), and higher-order KdV models is tested to describe large amplitude ISWs in the MAB. Based on …

The Mechanisms Of Synaptic Maintenance, Shaoyun Zang

Open Access Dissertations

Synapses are specialized cell-cell contacts where signals are reliably transduced from a neuron to its target cell in a regulated manner. Structurally, synapses are comprised of the pre- and post-synaptic terminals, and the synaptic cleft. Within each of these compartments is a complex network of molecular machinery, the components of which require constant turnover and “maintenance” to ensure a consistent relay of information. In neurodegenerative diseases, such as Alzheimer’s (AD), Parkinson’s (PD), and prion-related diseases, synaptic degeneration is often an early event associated with disease severity, which is evinced by the degree of cognitive decline. A dysfunction of synaptic maintenance …

An Exploratory Study: The Use Of Privately Owned Vessels As Mobile Research Platforms, Brittany L. Stockman

Open Access Theses

Due to limited public funding for scientific studies and data collection, private sector entities increasingly fill the gap by facilitating research inquiries. Specifically, coastal, oceanic and marine conservation nonprofit organizations (NPOs) play a direct role in research by both funding and orchestrating research missions across the globe. Through the examination of existing organizations and vessels involved in such efforts, this study examined the practicality of a NPO managed program that provides marine scientists with suitable mobile research platforms and evaluated the overall benefits of such a program on the ocean research community. One such organization, the International SeaKeepers Society (SeaKeepers), …

Mangrove Mitigation: A Case Study Of The Cocoplum Development, A History Of Land Use Changes And Trends In Mitigation Efforts, Stephanie J. Chaston

Open Access Theses

The residential community of the Islands of Cocoplum is located in Coral Gables, Florida along Biscayne Bay. It has served as a case study for mangrove mitigation techniques employed by Miami-Dade County's Regulatory and Economic Resources (RER) Coastal Resources division as a way of combating mangrove destruction. A database was created using Microsoft Excel and ArcGIS to identify and map all Class I coastal construction permits and mangrove violations since the onset of the Cocoplum Development in the 1980's through 2011. By identifying the mitigation strategies used by the County, it can be determined if mangrove mitigation effort has lessened, …

Engineering Analysis Of Minimally Invasive Mitral Valve Repair, Thuy Pham

Doctoral Dissertations

Surgical treatment for severe functional MR often involves mitral annuloplasty to improve leaflet apposition and ultimately downsize the dilated mitral valve. However, the high rate of operative mortalities of up to 6 ~12% have limited the more expanded use of this procedure. Recently, minimally invasive percutaneous transvenous mitral annuloplasty (PTMA) approaches using entirely catheter-based methods have been developed to reduce procedural morbidity and mortality. One of the approaches is to utilize the proximal location of the coronary sinus (CS) to the mitral annulus (MA) to percutaneously deploy a PTMA device within CS vessel. When the device contracts, it indirectly reshapes …

Insights Into Distinct Modulation Of α7 And α7β2 Nicotinic Acetylcholine Receptors By The Volatile Anesthetic, David D. Mowrey, Qiang Liu, Vasyl Bondarenko, Qiang Chen, Edom Seyoum, Yan Xu, Jie Wu, Pei Tang

Translational Neuroscience

Nicotinic acetylcholine receptors (nAChRs) are targets of general anesthetics, but functional sensitivity to anesthetic inhibition varies dramatically among different subtypes of nAChRs. Potential causes underlying different functional responses to anesthetics remain elusive. Here we show that in contrast to the α7 nAChR, the α7β2 nAChR is highly susceptible to inhibition by the volatile anesthetic isoflurane in electrophysiology measurements. Isoflurane-binding sites in β2 and α7 were found at the extracellular and intracellular end of their respective transmembrane domains using NMR. Functional relevance of the identified β2 site was validated via point mutations and subsequent functional measurements. Consistent with their functional responses …

Genetic Analysis Of The Replication Protein A Large Subunit Family In Arabidopsis Reveals Unique And Overlapping Roles In Dna Repair, Meiosis And Dna Replication, Behailu B. Aklilu, Ryan S. Soderquist, Kevin M. Culligan

New Hampshire Agricultural Experiment Station Publications

Replication Protein A (RPA) is a heterotrimeric protein complex that binds single-stranded DNA. In plants, multiple genes encode the three RPA subunits (RPA1, RPA2 and RPA3), including five RPA1-like genes in Arabidopsis. Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group). ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation. Analysis of rpa1 T-DNA insertion mutants demonstrates that although each mutant line is likely null, all mutant lines are viable and display normal vegetative growth. The rpa1c and …

[2013-12-13] Meeting Of The Academic And Health Affairs Policy Committee, Virginia Commonwealth University. Board Of Visitors

VCU Board of Visitors Minutes

Official minutes of the VCU Board of Visitors and its Executive Committee.

[2013-12-13] Meeting Of The Board Of Visitors Of Virginia Commonwealth University, Virginia Commonwealth University. Board Of Visitors

VCU Board of Visitors Minutes

Official minutes of the VCU Board of Visitors and its Executive Committee.

Lines, Sines, And Wallabies: The Dynamic Landscape Of A Regional Centromere, Laura E. Hall

Doctoral Dissertations

Archival abstract submitted

Outstanding Educational Performance Awards: Highlighting High-Achieving Arkansas Schools, 2013, Vera R. Deberg, Sarah M. Burks, Prairey Walkling, Gary W. Ritter

Arkansas Education Reports

The GPA measure, we believe, is a better representation of student achievement on statewide standardized exams. In this report, we are presenting a list of the top 25 schools in each area. In some cases, these "top 25 lists" will contain more than 25 schools as some schools' GPA scores will be identical. This is not a new phenomenon, as we also exceeded 25 schools in previous reports when using the percent proficient and advanced metric as an indicator for student achievement; however, there are fewer ties using the more precise GPA measure.

After we present our overall snapshot of …

Cellular Roles Of Dna Polymerase Beta, Sreerupa Ray, Miriam-Rose Menezes, Ali Senejani, Joann Balazs Sweasy

Biology and Environmental Science Faculty Publications

Since its discovery and purification in 1971, DNA polymerase ß (Pol ߆) is one of the most well-studied DNA polymerases. Pol ß is a key enzyme in the base excision repair (BER) pathway that functions in gap filling DNA synthesis subsequent to the excision of damaged DNA bases. A major focus of our studies is on the cellular roles of Pol ß. We have shown that germline and tumor-associated variants of Pol ß catalyze aberrant BER that leads to genomic instability and cellular transformation. Our studies suggest that Pol ß is critical for the maintenance of genomic stability and that …

Agenda And Minutes, University Of South Florida St. Petersburg General Education Committee Meeting, December 13, 2013, University Of South Florida St. Petersburg. Faculty Senate. General Education Committee

USF St. Petersburg General Education Committee Meetings

Agenda and minutes from the December 13, 2013, meeting of the General Education Committee of the University of South Florida St. Petersburg's Faculty Senate.

Ucc Minutes Dec 13, 2013, Undergraduate Curriculum Council

Undergraduate Currirculum Committee Minutes

No abstract provided.

Molecular Evolution Of Protein-Rna Mimicry As A Mechanism For Translational Control, Assaf Katz, Lindsey Solden, S. Betty Zou, William Wiley Navarre, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Elongation factor P (EF-P) is a conserved ribosome-binding protein that structurally mimics tRNA to enable the synthesis of peptides containing motifs that otherwise would induce translational stalling, including polyproline. In many bacteria, EF-P function requires post-translational modification with (R)-β-lysine by the lysyl-tRNA synthetase paralog PoxA. To investigate how recognition of EF-P by PoxA evolved from tRNA recognition by aminoacyl-tRNA synthetases, we compared the roles of EF-P/PoxA polar contacts with analogous interactions in a closely related tRNA/synthetase complex. PoxA was found to recognize EF-P solely via identity elements in the acceptor loop, the domain of the protein that interacts with the …

Adult Learning And Development Theories And Principles Represented In Theater, Jeffrey Miele

Theses and Dissertations

The purpose of this study was to investigate how adult learning and development theories and principles are represented in theater. The study also investigated if there was significant evidence to support the idea of using dramatic works as an example to illustrate adult learning and development theories and principles as an educational tool. Ten plays were analyzed along with an accompanying rubric that the evaluator completed while reading the plays. The rubric searched for information if adults and/or educators were present in the text, how andragogy and barriers were presented, along with other theories and principles. The findings suggested that …

Certain Agave Species Exhibit The Capability To Be Moderately Productive Under Conditions Of High Salt And Drought Stress, Steven J. Bergsten

Theses and Dissertations

Water availability and arable lands are increasingly limiting resources in many parts of the U.S., particularly in semi-arid and arid regions. As a means of addressing food and fuel demands associated with burgeoning population growth, highly productive and water-use efficient crops need to be identified. One potential crop, Agave, merits consideration and evaluation due to its putative capability to provide sustenance and energy despite growing in water-limited regions and on marginal soils. However, little is known regarding the productivity these succulent plants will have under growing conditions of the Southwest, where high concentrated saline soils are abundant, and water is …

Toward Target 2 Of The Global Strategy For Plant Conservation: An Expert Analysis Of The Puerto Rican Flora To Validate New Streamlined Methods For Assessing Conservation Status, James S. Miller, Gary A. Krupnick, Hannah Stevens, Holly Porter-Morgan, Brian Boom, Pedro Acevedo-Rodríguez, James Ackerman, Duane Kolterman, Eugenio Santiago, Christian Torres, Jeanine Velez

Publications and Research

Target 2 of the 2020 Global Strategy for Plant Conservation (GSPC) calls for a comprehensive list of the world's threatened plant species. The lack of such a list is one of the greatest impediments to protecting the full complement of the world's plant species, and work to achieve this has been slow. An efficient system for identifying those species that are at risk of extinction could help to achieve this goal in a time frame sensitive to today's conservation needs. Two systems that efficiently use available data to assess conservation status were tested against a provisional International Union for Conservation …

2013 Fall Commencement Program: Winona State University, Winona State University

WSU Commencement Programs

This is the Winona State University Fall 2013 commencement program. Commencement was on Friday, December 13, 2013.

Luciana Ranelli '14 Looks To El Paso, University Relations

Campus News Archive

The biology and secondary education major is preparing to student teach in El Paso’s Ysleta Independent School District.