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A Protein (Orf2) Encoded By The Latency-Related Gene Of Bovine Herpesvirus 1 Interacts With Dna, Daraporn Pittayakhajonwut, Devis Sinani, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1), like other members of the Alphaherpesvirinae subfamily, establishes latency in sensory neurons. The virally encoded latency-related RNA (LR-RNA) is expressed abundantly in latently infected sensory neurons and encodes several proteins, including ORF2. An LR mutant virus with stop codons at the amino terminus of ORF2 does not reactivate from latency after treatment with the synthetic corticosteroid dexamethasone, in part because it induces higher levels of apoptosis during the establishment of latency. ORF2 inhibits apoptosis, interacts with three cellular transcription factors (Notch1, Notch3, and C/EBP-α), and interferes with Notch-mediated signaling. Consequently, we predict that ORF2 expression is …

A Bovine Herpesvirus 1 Protein Expressed In Latently Infected Neurons (Orf2) Promotes Neurite Sprouting In The Presence Of Activated Notch1 Or Notch3, Devis Sinani, Leticia Frizzo Da Silva, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1) infection induces clinical symptoms in the upper respiratory tract, inhibits immune responses, and can lead to life-threatening secondary bacterial infections. Following acute infection, BHV-1 establishes latency in sensory neurons within trigeminal ganglia, but stress can induce reactivation from latency. The latency-related (LR) RNA is the only viral transcript abundantly expressed in latently infected sensory neurons. An LR mutant virus with stop codons at the amino terminus of the first open reading frame (ORF) in the LR gene (ORF2) is not reactivated from latency, in part because it induces higher levels of apoptosis in infected neurons. ORF2 …

Deep Sequencing And Genome-Wide Analysis Reveals The Expansion Of Microrna Genes In The Gall Midge Mayetiola Destructor, Chitvan Khajuria, Christie E. Williams, Mustapha El Bouhssini, R. Jeff Whitworth, Stephen Richards, Jeffrey J. Stuart, Ming-Shun Chen

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect – host plant interactions.

Results: In this study, we systematically analyzed miRNAs from the Hessian fly. Deep-sequencing a Hessian fly larval transcriptome led to the identification of 89 miRNA species that are either identical or very …

Development And Evaluation Of A Replicon Particle Vaccine Expressing The E2 Glycoprotein Of Bovine Viral Diarrhea Virus (Bvdv) In Cattle, John Dustin Loy, Jill Gander, Mark Mogler, Ryan Vander Veen, Julia Ridpath, Delbert Hank Harris, Kurt Kamrud

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine viral diarrhea virus is one of the most significant and costly viral pathogens of cattle worldwide. Alphavirus-derived replicon particles have been shown to be safe and highly effective vaccine vectors against a variety of human and veterinary pathogens. Replicon particles are non-propagating, DIVA compatible, and can induce both humoral and cell mediated immune responses. This is the first experiment to demonstrate that Alphavirus-based replicon particles can be utilized in a standard prime/boost vaccination strategy in calves against a commercially significant bovine pathogen.

Findings: Replicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were …

Cultivation Conditions And The Diffusion Of Oxygen Into Culture Media: The Rationale For The Flask-To-Medium Ratio In Microbiology, Greg A. Somerville, Richard A. Proctor

School of Veterinary and Biomedical Sciences: Faculty Publications

Bacterial cultivation requires consideration of three things: The bacterial strain, cultivation medium, and cultivation conditions. Most microbiologists dutifully report their choice of strains and cultivation media in manuscripts; however, these same microbiologists often overlook reporting cultivation conditions. Without this information, it is difficult to determine if cultures were grown aerobically, microaerobically, or anaerobically. To cultivate bacteria aerobically, it is necessary to understand that oxygen does not readily diffuse into culture media; it needs help to get in. Microbiologists can do this by altering the flask-to-medium ratio, rpm of agitation, and/or the concentration of atmospheric oxygen, or by using baffled flasks.

Factors Affecting Survival And Cause-Specific Mortality Of Saiga Calves In Mongolia, Bayarbaatar Buuveibaatar, Julie K. Young, Joel Berger, Amanda E. Fine, Badamjav Lkhagvasuren, Peter Zahler, Todd K. Fuller

United States Department of Agriculture Wildlife Services: Staff Publications

Factors affecting juvenile survival are poorly known in the world’s most northern antelope, the endangered saiga (Saiga tatarica), yet these factors are fundamental for understanding what drives population change. We monitored Mongolia saiga (S. tatarica mongolica) calves in Sharga Nature Reserve, western Mongolia, during 2008–2010. Our results showed that male and single calves were heavier than females and twins, respectively. However, we identified no significant differences in seasonal or annual survival rates between sexes or between singletons and twins. Litter size and birth mass varied among years, and there was a negative relationship between these variables. …

Multidisciplinary Approach To Epizootiology And Pathogenesis Of Bat Rabies Viruses In The United States, J. A. Ellison, S. R. Johnson, N. Kuzmina, A. T. Gilbert, W. C. Carson, Kurt C. Vercauteren, C. E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Zoonotic disease surveillance is typically initiated after an animal pathogen has caused disease in humans. Early detection of potentially high-risk pathogens within animal hosts may facilitate medical interventions to cope with an emerging disease. To effectively spillover to a novel host, a pathogen may undergo genetic changes resulting in varying transmission potential in the new host and potentially to humans. Rabies virus (RABV) is one model pathogen to consider for studying the dynamics of emerging infectious diseases under both laboratory and field conditions. The evolutionary history of RABV is characterized by regularly documented spillover infections and a series of notable …

Immunodetection Of Triticum Mosaic Virus By Das- And Dac-Elisa Using Antibodies Produced Against Coat Protein Expressed In Escherichia Coli: Potential For High-Throughput Diagnostic Methods, Satyanarayana Tatineni, Gautam Sarath, Dallas Seifers, Roy C. French

Department of Agronomy and Horticulture: Faculty Publications

Triticum mosaic virus (TriMV), an economically important virus infecting wheat in the Great Plains region of the USA, is the type species of the Poacevirus genus in the family Potyviridae. Sensitive and high-throughput serology-based detection methods are crucial for the management of TriMV and germplasm screening in wheat breeding programs. In this study, TriMV coat protein (CP) was expressed in Escherichia coli, and polyclonal antibodies were generated against purified soluble native form recombinant CP (rCP) in rabbits. Specificity and sensitivity of resulting antibodies were tested in Western immuno-blot and enzyme-linked immunosorbent assays (ELISA). In direct antigen coating (DAC)-ELISA, …

Soil Sorptivity Enhancement With Crop Residue Accumulation In Semiarid Dryland No-Till Agroecosystems, T.M. Shaver, G. A. Peterson, Laj R. Ahuja, D.G. Westfall

Department of Agronomy and Horticulture: Faculty Publications

Water capture and precipitation use efficiency are of great importance in dryland cropping systems because the world's dependence on food produced in dryland areas continues to increase. Growing season evapotranspiration potential greatly exceeds growing season precipitation rates in dryland areas, creating a water deficit for crops. Management practices that positively impact soil physical properties increase the potential for soils to capture water. One way to assess the ability of soils to capture water is through the measurement of sorptivity. Sorptivity is defined as the cumulative infiltration proportionality constant and is governed by surface soil physical properties such as texture, degree …

Enzyme Activity In Wheat Breeding Lines Derived From Matings Of Low Polyphenol Oxidase Parents, Somrudee Nilthong, Robert A. Graybosch, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Polyphenol oxidase (PPO) in grain plays a major role in time-dependent discoloration of wheat (Triticum aestivum L.) products, especially fresh noodles. Breeding wheat cultivars with low or nil PPO activity can reduce undesirable product darkening. The low PPO line PI 117635 was crossed to two low PPO wheats, IDO580 and ‘IDO377s’, to determine whether matings between wheats with low levels of grain PPO would result in complementation, such that lines with still lower or nil PPO would be generated. Progeny in a population derived from PI 117635/ IDO580 displayed no variation in PPO activity. In the F_3:4 populations …

Evaluation Of Public Sweet Sorghum A-Lines For Use In Hybrid Production, Jeffrey F. Pedersen, Scott E. Sattler, William F. Anderson

Department of Agronomy and Horticulture: Faculty Publications

A fundamental need for commercialization of sweet sorghum [Sorghum bicolor (L.) Moench] as a bioenergy crop is an adequate seed supply, which will require development of hybrid varieties using dwarf seed-parent lines. A set of six public sweet sorghum A-lines (Dwarf Kansas Sourless, KS9, N36, N38, N39, and N4692) were crossed with a set of six public sweet sorghum cultivars (Brawley, Kansas Collier, Dale, Sugar Drip, Waconia, and Wray). Grain, fiber, and sugar yields were determined, and conversion formulas were applied to estimate ethanol yields. Hybrids were grown in fields at Ithaca, NE, USA, in 1983– 1984 fertilized with …

Functional Characterization Of Cinnamyl Alcohol Dehydrogenase And Caffeic Acid O-Methyltransferase In Brachypodium Distachyon, Gina M. Trabucco, Dominick A. Matos, Scott J. Lee, Aaron J. Saathoff, Henry D. Priest, Todd C. Mockler, Gautam Sarath, Samuel P. Hazen

Department of Agronomy and Horticulture: Faculty Publications

Background: Lignin is a significant barrier in the conversion of plant biomass to bioethanol. Cinnamyl alcohol dehydrogenase (CAD) and caffeic acid O-methyltransferase (COMT) catalyze key steps in the pathway of lignin monomer biosynthesis. Brown midrib mutants in Zea mays and Sorghum bicolor with impaired CAD or COMT activity have attracted considerable agronomic interest for their altered lignin composition and improved digestibility. Here, we identified and functionally characterized candidate genes encoding CAD and COMT enzymes in the grass model species Brachypodium distachyon with the aim of improving crops for efficient biofuel production.

Results: We developed transgenic plants overexpressing artificial microRNA designed …

Characterization Of Fluorescent Pseudomonas Spp. Associated With Roots And Soil Of Two Sorghum Genotypes, Deanna L. Funnell-Harris, Scott E. Sattler, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Sorghum is used as bioenergy feedstock, animal feed, and food. Economical methods for disease prevention and control are valuable for producers. Fluorescent Pseudomonas spp. were isolated from sorghum roots and surrounding soil with the goal of finding isolates that significantly inhibited sorghum fungal pathogens. Fluorescent pseudomonads were collected from seedlings of sorghum cultivars RTx433 and Redlan and wheat cultivar Lewjain, grown in two soils. Lewjain is known to support growth of producers of the antibiotic, 2,4-diacetylphloroglucinol (2,4-DAPG). Isolates from all three plants were assessed for hydrogen cyanide (HCN) and extracellular protease production, and for a 2,4-DAPG gene, phlD. Both …

Transcriptome Analysis Of Two Buffalograss Cultivars, Michael Wachholtz, Tiffany Heng-Moss, Paul Twigg, Lisa Baird, Guoqing Lu, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

Background: Buffalograss [Buchloë dactyloides (Nutt.) Engel. syn. Bouteloua dactyloides (Nutt.) Columbus] is a United States native turfgrass species that requires less irrigation, fungicides and pesticides compared to more commonly used turfgrass species. In areas where water is limited, interest in this grass species for lawns is increasing. While several buffalograss cultivars have been developed through buffalograss breeding, the timeframe for new cultivar development is long and is limited by a lack of useful genetic resources. Two high throughput next-generation sequencing techniques were used to increase the genomic resources available for buffalograss.

Results: Total RNA was extracted and …

Complete Plastid Genomes From Ophioglossum Californicum, Psilotum Nudum, And Equisetum Hyemale Reveal An Ancestral Land Plant Genome Structure And Resolve The Position Of Equisetales Among Monilophytes, Felix Grewe, Wenhu Guo, Emily A. Gubbels, A Katie Hansen, Jeffrey P. Mower

Department of Agronomy and Horticulture: Faculty Publications

Background: Plastid genome structure and content is remarkably conserved in land plants. This widespread conservation has facilitated taxon-rich phylogenetic analyses that have resolved organismal relationships among many land plant groups. However, the relationships among major fern lineages, especially the placement of Equisetales, remain enigmatic.

Results: In order to understand the evolution of plastid genomes and to establish phylogenetic relationships among ferns, we sequenced the plastid genomes from three early diverging species: Equisetum hyemale (Equisetales), Ophioglossum californicum (Ophioglossales), and Psilotum nudum (Psilotales). A comparison of fern plastid genomes showed that some lineages have retained inverted repeat (IR) boundaries originating …

Indoor Application Of Attractive Toxic Sugar Bait (Atsb) In Combination With Mosquito Nets For Control Of Pyrethroid-Resistant Mosquitoes, Zachary P. Stewart, Richard M. Oxborough, Patrick K. Tungu, Matthew J. Kirby, Mark W. Rowland, Seth R. Irish

Department of Agronomy and Horticulture: Faculty Publications

Background: Attractive toxic sugar bait (ATSB) sprayed onto vegetation has been successful in controlling Anopheles mosquitoes outdoors. Indoor application of ATSB has yet to be explored. The purpose of this study was to determine whether ATSB stations positioned indoors have the potential to kill host-seeking mosquitoes and constitute a new approach to control of mosquito-borne diseases.

Methods: Insecticides were mixed with dyed sugar solution and tested as toxic baits against Anopheles arabiensis, An. Gambiae s.s. and Culex quinquefasciatus in feeding bioassay tests to identify suitable attractant-insecticide combinations. The most promising ATSB candidates were then trialed in experimental …

Sorghum Transformation: Overview And Utility, Tejinder Kumar, Arlene Howe, Shirley Sato, Ismail M. Dweikat, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Over the past decade genomics resources available for sorghum have rapidly expanded (Paterson Int J Plant Genomics 2008:6, 2008), these resources, coupled with the recent completion of the genome sequence which is relatively small in size (730 Mb) (Paterson et al. Nature 457:551–556, 2009) makes sorghum a rather attractive species to study. Moreover, the USDA germplasm system maintains 42,614 accessions, of which more than 800 exotic landraces have been converted to day length-insensitive lines to facilitate their use in breeding programs. In addition, a set of EMS mutation stocks developed by the USDA Plant Stress and Germplasm Development Unit in …

Self-Assembling Of Gold Nanoparticles Array For Electro-Sensing Applications, Islam M. Al-Akraa Dr, Ahmad M. Mohammad Prof, Mohamed S. El-Deab Prof, Bahgat E. El-Anadouli Prof

Chemical Engineering

A colloidal solution of citrate-stabilized gold nanoparticles (AuNPs) with an average size of ca. 2.6 nm has been prepared, characterized and further implemented in electro-sensing applications. This colloidal solution of AuNPs has been prepared via the reduction of NaAuCl4 with sodium tetrahydroborate (NaBH4) using trisodium citrate as a stabilizer. The optical properties of this solution have been studied with UV–Vis spectroscopy. Next, these AuNPs have been immobilized onto a polycrystalline Au (poly-Au) electrode with the assistance of benzenedimethanethiol (BDMT), which served as a binder. Attention has been taken to ensure the formation of a compact impermeable layer of BDMT on …

A Fully Defined And Scalable 3d Culture System For Human Pluripotent Stem Cell Expansion And Differentiation, Yuguo Lei, David V. Schaffer

Department of Chemical and Biomolecular Engineering: Faculty Publications

Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are promising for numerous biomedical applications, such as cell replacement therapies, tissue and whole-organ engineering, and high-throughput pharmacology and toxicology screening. Each of these applications requires large numbers of cells of high quality; however, the scalable expansion and differentiation of hPSCs, especially for clinical utilization, remains a challenge. We report a simple, defined, efficient, scalable, and good manufacturing practice-compatible

3D culture system for hPSC expansion and differentiation. It employs a thermoresponsive hydrogel that combines easy manipulation and completely defined conditions, free of any human- or …

Mechanotransduction Of Ultrasound Is Frequency Dependent Below The Cavitation Threshold, Tobias M. Louw, Gaurav Budhiraja, Hendrik J. Viljoen, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

This study provides evidence that low-intensity ultrasound directly affects nuclear processes, and the magnitude of the effect varies with frequency. In particular, we show that the transcriptional induction of first load-inducible genes, which is independent of new protein synthesis, is frequency dependent. Bovine chondrocytes were exposed to low-intensity below the cavitational threshold) ultrasound at 2,5 and 8 MHz. Ultrasound elevated the expression of early response genes c-Fos, c-Jun and c-Myc, maximized at 5 MHz. The phosphorylated ERK inhibitor PD98059 abrogated any increase in c-series gene expression, suggesting that signaling occurs via the MAPPK/ERK pathway. However, phosphorylated ERK levels did …

New Potential Therapeutic Approach For The Treatment Of B-Cell Malignancies Using Chlorambucil/ Hydroxychloroquine-Loaded Anti-Cd20 Nanoparticles, Nelly Mezzaroba, Sonia Zorzet, Erika Secco, Stefania Biffi, Claudio Tripodo, Marco Calvaruso, Ramiro Mendoza-Maldonado, Sara Capolla, Marilena Granzotto, Ruben Spretz, Gustavo F. Larsen, Sandra Noriega, Marianna Lucafó, Eduardo Mansilla, Chiara Garrovo, Gustavo H. Marín, Gabriele Bai, Valter Gattei, Gabriele Pozzato, Luis Núñez, Paolo Macor

Department of Chemical and Biomolecular Engineering: Faculty Publications

Current B-cell disorder treatments take advantage of dose-intensive chemotherapy regimens and immunotherapy via use of monoclonal antibodies. Unfortunately, they may lead to insufficient tumor distribution of therapeutic agents, and often cause adverse effects on patients. In this contribution, we propose a novel therapeutic approach in which relatively high doses of Hydroxychloroquine and Chlorambucil were loaded into biodegradable nanoparticles coated with an anti-CD20 antibody. We demonstrate their ability to effectively target and internalize in tumor B-cells. Moreover, these nanoparticles were able to kill not only p53 mutated/deleted lymphoma cell lines expressing a low amount of CD20, but also circulating primary cells …

Ultrasonic Bioreactor As A Platform For Studying Cellular Response, Anuradha Subramanian, Joseph A. Turner, Gaurav Budhiraja, Sanjukta Guha Thakurta, Nicholas P. Whitney, Sai Siddhartha Nudurupati

Department of Chemical and Biomolecular Engineering: Faculty Publications

The need for tissue-engineered constructs as replacement tissue continues to grow as the average age of the world’s population increases. However, additional research is required before the efficient production of laboratory-created tissue can be realized. The multitude of parameters that affect cell growth and proliferation is particularly daunting considering that optimized conditions are likely to change as a function of growth. Thus, a generalized research platform is needed in order for quantitative studies to be conducted. In this article, an ultrasonic bioreactor is described for use in studying the response of cells to ultrasonic stimulation. The work is focused on …

Refining Thresholds In Coupled Fire–Vegetation Models To Improve Management Of Encroaching Woody Plants In Grasslands, Dirac L. Twidwell Jr, Samuel D. Fuhlendorf, Charles A. Taylor Jr, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

1. Restoration priorities are typically established without quantitative information on how to overcome the thresholds that preclude successful restoration of desirable ecosystem properties and services. We seek to demonstrate that quantifying ecological thresholds and incorporating them into management-oriented frameworks provide a more comprehensive perspective on how the threshold concept can be applied to achieve restoration goals.

2. As an example, restoration actions have been largely unsuccessful when based on prevailing ecological knowledge of fire-based thresholds in nonresprouting Juniperus woodland. We build on previous threshold-based research and link well-established models from applied fire physics with a widely applied ecological positive feedback …

Nutrient Partitioning And Grain Yield Of Tanam-Rnai Wheat Under Abiotic Stress, Mary J. Guttieri, Ricardo J. Stein, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Aims — Decreased expression of TaNAM genes by RNAi results in delayed senescence and decreased grain protein, iron, and zinc concentrations. Here, we determined whether NAM expression level alters onset of senescence under stress conditions, whether delayed senescence in the TaNAM-RNAi line resulted in improved tolerance to post-anthesis abiotic stress, and determined the effects of post-anthesis abiotic stress on N and mineral remobilization and partitioning to grain.

Methods — Greenhouse-grown WT and TaNAM-RNAi wheat were characterized in two studies: three levels of N fertility or water limitation during grain fill. Studies were conducted under both optimal and heat stress …

The Essentials Of Economic Sustainability By John Ikerd [Book Review], Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Essentials of Economic Sustainability is the latest challenging statement on innovative economic philosophy from an alternative opinion leader John Ikerd, retired agricultural economist from University of Missouri, Columbia (Columbia, Missouri, United States). His perspective is that accelerating changes surrounding us require more than small adaptations, but rather a thoughtful reconsideration of basic principles about how the world works and our role in this dynamic reality. Ikerd paraphrases the challenge of the 1988 Brundtland Report of the United Nations that we must design strategies ‘to meet the economic needs of the present without diminishing economic opportunities for the future’ (page 1). …

The Challenges Of Growing Strawberries In The Greenhouse, Ellen T. Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

As a floriculturist, when I first decided to grow strawberries (Fragaria x ananassa) in the greenhouse, I thought it would be a snap. After all, I could practice what I preach to my classes in that I would use all the sustainable growing tricks from floriculture, create a production time line and it would be ready, set grow. However, moving a field-grown summer crop into a greenhouse as a winter crop was not the same as moving a winter greenhouse-grown crop outside for the summer. Not only were the plants typically grown in lush field soil, but also …

Genetic Mapping And Confirmation Of Quantitative Trait Loci For Seed Protein And Oil Contents And Seed Weight In Soybean, Safiullah M. Pathan, Tri Vuong, Kerry Clark, Jeong-Dong Lee, J. Grover Shannon, Craig A. Roberts, Mark R. Ellersieck, Joseph W. Burton, P. B. Cregan, David L. Hyten, Henry T. Nguyen, David A. Sleper

Department of Agronomy and Horticulture: Faculty Publications

Demand for soybean [Glycine max (L.) Merr.] meal has increased worldwide and soybean importers often offer premiums for soybean containing higher contents of protein and oil. Objectives were to detect quantitative trait loci (QTL) associated with soybean seed protein, oil, and seed weight in a soybean mapping population and confirm detected QTL across genetic backgrounds and environments. Two populations of 216 and 156 recombinant inbred lines were developed from Magellan × PI 438489B and Magellan × PI 567516C crosses, respectively, and grown in two Missouri environments in 2008. More than 900 simple sequence repeat (SSR) and single nucleotide polymorphism …

Soybean Seed Amino Acid Content Qtl Detected Using The Universal Soy Linkage Panel 1.0 With 1,536 Snps, Benjamin D. Fallen, Catherine N. Hatcher, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is the primary source of meal used in animal feed in the U.S. However, few studies have been conducted to evaluate genomic regions controlling amino acid composition is soybean. Designing soybean seed compositions that will benefit animal production is essential. The objective of this study was to identify genomic regions controlling essential and non-essential amino acid composition in soybean seed proteins. To achieve this objective, 282 F_5:9 recombinant inbred lines (RILs) developed from a cross of Essex × Williams 82 were used. Ground soybean seed samples were analyzed for amino acids and statistically …

Development And Evaluation Of Soysnp50k, A High-Density Genotyping Array For Soybean, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The objective of this research was to identify single nucleotide polymorphisms (SNPs) and to develop an Illumina Infinium BeadChip that contained over 50,000 SNPs from soybean (Glycine max L. Merr.). A total of 498,921,777 reads 35–45 bp in length were obtained from DNA sequence analysis of reduced representation libraries from several soybean accessions which included six cultivated and two wild soybean (G. soja Sieb. et Zucc.) genotypes. These reads were mapped to the soybean whole genome sequence and 209,903 SNPs were identified. After applying several filters, a total of 146,161 of the 209,903 SNPs were determined to be …

The Plant Science Decadal Vision: Response To The Martin Commentary, David Stern, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

The plant science community is being called upon as never before to carry out research that addresses challenges in food, climate, and health, to educate a populace inundated with science misinformation, and to excite and recruit a new generation of scientists from among that populace, all while investments in basic research are stagnant or even declining in many countries. The Decadal Vision (Plant Science Research Summit, 2013) represents a response from that community, one that launches a meaningful and ongoing dialogue about how best to accomplish these goals. In that spirit, we welcome this thoughtful commentary by Cathie Martin (Martin, …