Digital Commons Network^™

Resistive-Pulse Measurements With Nanopipettes: Detection Of Au Nanoparticles And Nanoparticle-Bound Anti-Peanut Igy†, Vigneshwaran Mani, Naimish Sardesai, James F. Rusling

UCHC Articles - Research

Solid-state nanopores have been widely employed in sensing applications from Coulter counters to DNA sequencing devices. The analytical signal in such experiments is the change in ionic current flowing through the orifice caused by the large molecule or nanoparticle translocation through the pore. Conceptually similar nanopipette-based sensors can offer several advantages including the ease of fabrication and small physical size essential for local measurements and experiments in small spaces. This paper describes the first evaluation of nanopipettes with well characterized geometry for resistive-pulse sensing of Au nanoparticles (AuNP), nanoparticles coated with an allergen epitope peptide layer, and AuNP–peptide particles with …

Transient Receptor Potential (Trp) Channels And Cardiac Fibrosis, Zhichao Yue, Yanhui Zhang, Jia Xie, Jianmin Jiang, Lixia Yue

UCHC Articles - Research

Cardiac fibrosis is associated with most cardiac diseases. Fibrosis is an accumulation of excessive extracellular matrix proteins (ECM) synthesized by cardiac fibroblasts and myofibroblasts. Fibroblasts are the most prevalent cell type in the heart, comprising 75% of cardiac cells. Myofibroblasts are hardly present in healthy normal heart tissue, but appear abundantly in diseased hearts. Cardiac fibroblasts are activated by a variety of pathological stimuli, such as myocardial injury, oxidative stress, mechanical stretch, and elevated autocrine-paracrine mediators, thereby undergoing proliferation, differentiation to myofibroblasts, and production of various cytokines and ECM proteins. A number of signaling pathways and bioactive molecules are involved …

Coordination Of Peroxide To The Cum Center Of Peptidylglycine Α-Hydroxylating Monooxygenase (Phm): Structural And Computational Study, Betty A. Eipper, Richard E. Mains

UCHC Articles - Research

Many bioactive peptides, such as hormones and neuropeptides, require amidation at the C terminus for their full biological activity. Peptidylglycine α-hydroxylating monooxygenase (PHM) performs the first step of the amidation reaction—the hydroxylation of peptidylglycine substrates at the Cα position of the terminal glycine. The hydroxylation reaction is copper- and O₂-dependent and requires 2 equiv of exogenous reductant. The proposed mechanism suggests that O₂ is reduced by two electrons, each provided by one of two nonequivalent copper sites in PHM (Cu_H and Cu_M). The characteristics of the reduced oxygen species in the PHM reaction and …

Volatility In Mrna Secondary Structure As A Design Principle For Antisense, Erik Johnson, Ranjan Srivastava

Open Access Author Fund Awardees' Articles

Designing effective antisense sequences is a formidable problem. A method for predicting efficacious antisense holds the potential to provide fundamental insight into this biophysical process. More practically, such an understanding increases the chance of successful antisense design as well as saving considerable time, money and labor. The secondary structure of an mRNA molecule is believed to be in a constant state of flux, sampling several different suboptimal states. We hypothesized that particularly volatile regions might provide better accessibility for antisense targeting. A computational framework, GenAVERT was developed to evaluate this hypothesis. GenAVERT used UNAFold and RNAforester to generate and compare …

Behavioural Salinity Preferences Of Juvenile Green Sturgeon Acipenser Medirostris Acclimated To Fresh Water And Full-Strength Salt Water, J. B. Poletto, D. E. Cocherell, A. P. Klimley, J. J. Cech Jr., N. A. Fangue

School of Natural Resources: Faculty Publications

To quantify the salinity preference of juvenile green sturgeon Acipenser medirostris, two groups of A. medirostris [140 days post hatch (dph); total length (L_T) 38.0–52.5 cm] were acclimated to either near fresh water (mean ± S.E. salinity = 3.2 ± 0.6) or full-strength salt water (34.1 ± 1.2) over 8 weeks. Following acclimation, the two groups were divided into experimental and control groups, where experimental A. medirostris from both freshwater and saltwater acclimations were individually introduced (200–220 dph) into a rectangular salinity-preference flume (maximum salinity gradient: 5–33). Control A. medirostris were presented with only their acclimation …

Removal Of Pb(Ii) From Aqueous Solution By A Zeolite–Nanoscale Zero-Valent Iron Composite, Seol Ah Kim, Seralathan Kamala-Kannan, Kui-Jae Lee, Yool-Jin Park, Patrick J. Shea, Wang-Hyu Lee, Hyung-Moo Kim, Byung-Taek Oh

School of Natural Resources: Faculty Publications

The effectiveness of nanoscale zero-valent iron (nZVI) to remove heavy metals from water is reduced by its low durability, poor mechanical strength, and tendency to form aggregates. A composite of zeolite and nanoscale zero-valent iron (Z–nZVI) overcomes these problems and shows good potential to remove Pb from water. FTIR spectra support nZVI loading onto the zeolite and reduced Fe⁰ oxidation in the Z–nZVI composite. Scanning electron micrographs show aggregation was eliminated and transmission electron micrographs show well-dispersed nZVI in chain-like structures within the zeolite matrix. The mean surface area of the composite was 80.37 m²/g, much greater …

Ethanol Injection Of Ornamental Trees Facilitates Testing Insecticide Efficacy Against Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae), Michael E. Reding, Jason B. Oliver, Peter B. Schultz, Christopher M. Ranger, Nadeer N. Youssef

Agricultural and Environmental Sciences Faculty Research

Exotic ambrosia beetles are damaging pests in ornamental tree nurseries in North America. The species Xylosandrus crassiusculus (Motshulsky) and Xylosandrus germanus (Blandford) are especially problematic. Management of these pests relies on preventive treatments of insecticides. However, field tests of recommended materials on nursery trees have been limited because of unreliable attacks by ambrosia beetles on experimental trees. Ethanol-injection of trees was used to induce colonization by ambrosia beetles to evaluate insecticides and botanical formulations for preventing attacks by ambrosia beetles. Experiments were conducted in Ohio, Tennessee, and Virginia. Experimental trees injected with ethanol had more attacks by ambrosia beetles than …

A Positive Feedback Loop Regulates The Expression Of Polycomb Group Protein Bmi1 Via Wnt Signaling Pathway, Joon-Ho Cho, Manjari Dimri, Goberdhan P. Dimri

Biochemistry and Molecular Medicine Faculty Publications

Polycomb group protein BMI1 plays an important role in cellular homeostasis by maintaining a balance between proliferation and senescence. It is often overexpressed in cancer cells and is required for self-renewal of stem cells. At present, very little is known about the signaling pathways that regulate the expression of BMI1. Here, we report that BMI1 autoactivates its own promoter via an E-box present in its promoter. We show that BMI1 acts as an activator of the WNT pathway by repressing Dickkopf (DKK) family of WNT inhibitors. BMI1 mediated repression of DKK proteins; in particular, DKK1 led to up-regulation of WNT …

Crowdsourcing With Arcgis Online For Natural Resources Management, Yanli Zhang, Matthew W. Mcbroom, Jason Grogan, Paul R. Blackwell

Faculty Publications

No abstract provided.

Isolation Of A Novel Swine Influenza Virus From Oklahoma In 2011 Which Is Distantly Related To Human Influenza C Viruses, Ben Hause, Mariette Ducatez, Emily A. Collin, Zhiguang Ran, Runxia Liu, Zizhang Sheng, Anibal Armien, Bryan Kaplan, Suvobrata Chakravarty, Adam D. Hoppe, Richard J. Webby, Randy R. Simonson, Feng Li

Chemistry and Biochemistry Faculty Publications

Of the Orthomyxoviridae family of viruses, only influenza A viruses are thought to exist as multiple subtypes and has nonhuman maintenance hosts. In April 2011, nasal swabs were collected for virus isolation from pigs exhibiting influenza-like illness. Subsequent electron microscopic, biochemical, and genetic studies identified an orthomyxovirus with seven RNA segments exhibiting approximately 50% overall amino acid identity to human influenza C virus. Based on its genetic organizational similarities to influenza C viruses this virus has been provisionally designated C/Oklahoma/1334/2011 (C/OK). Phylogenetic analysis of the predicted viral proteins found that the divergence between C/OK and human influenza C viruses was …

Progress Notes, Lehigh Valley Health Network

Progress Notes

No abstract provided.

Scholarworks Statistics - February 2013, Scholarworks

ScholarWorks Reports

For the month of February 2013, ScholarWorks recorded a total of 24,199 full-text downloads and 14,122 page views.

The Future Of Legal Education Reform, James E. Moliterno

Scholarly Articles

The article discusses the criticism raised against legal education including high cost, disconnection between law schools and profession, and lack of employment opportunities. It examines the role of the bar examinations and reflects that the model in place is dysfunctional. It suggests that modern law school should teach students not only legal analysis but also business aspect of law practice such as project management and creative resolutions of disputes.

Examination Of Ulva Bloom Species Richness And Relative Abundance Reveals Two Cryptically Co- Occurring Bloom Species In Narragansett Bay, Rhode Island, Michele Guidone, Carol S. Thornber

Biological Sciences Faculty Publications

Blooms caused by the green macroalga Ulva pose a serious threat to coastal ecosystem 20 around the world. Despite numerous studies of the causes and consequences of these blooms, we still have a limited understanding of Ulva bloom species richness and abundance due to difficulties in identifying Ulva species using morphological features. Along the northeastern U.S. coastline, all blooms of distromatic Ulva blades were previously identified as U. lactuca. Recent molecular sequencing, however, discovered the presence of additional distromatic Ulva species. Therefore, in order to determine the relative abundance of Ulva species within blooms, we conducted monthly surveys at four …

E-Learning In Medical Education In Resource Constrained Low- And Middle-Income Countries, Seble Frehywot, Yianna Vovides, Zohray Talib, Nadia Mikhail, Heather Ross, Hannah Wohltjen, Selam Bedada, Kristine Korhumel, Abdel Karim Koumare, James Scott

Health Policy and Management Faculty Publications

Background

In the face of severe faculty shortages in resource-constrained countries, medical schools look to e-learning for improved access to medical education. This paper summarizes the literature on e-learning in low- and middle-income countries (LMIC), and presents the spectrum of tools and strategies used.

Methods

Researchers reviewed literature using terms related to e-learning and pre-service education of health professionals in LMIC. Search terms were connected using the Boolean Operators “AND” and “OR” to capture all relevant article suggestions. Using standard decision criteria, reviewers narrowed the article suggestions to a final 124 relevant articles.

Results

Of the relevant articles found, most …

The Crater Special Issue Of Space Weather: Building The Observational Foundation To Deduce Biological Effects Of Space Radiation, Nathan A. Schwadron, Sonya S. Smith, Harlan E. Spence

Physics & Astronomy

[1] The United States is preparing for exploration beyond low-Earth Orbit (LEO). However, the space radiation environment poses significant risks. The radiation hazard is potentially severe but not sufficiently well characterized to determine if long missions outside LEO can be accomplished with acceptable risk [Cucinotta et al., 2001; Schwadron et al., 2010; Cucinotta et al., 2010]. Radiation hazards may be over- or under-stated through incomplete characterization in terms of net quantities such as accumulated dose. Time-dependent characterization often changes acute risk estimates [NCRP, 1989; Cucinotta, 1999; Cucinotta et al., 2000; George et al., 2002]. For example, events with high accumulated …

Blood Oxidative-Stress Markers During A High-Altitude Trek, Lindsey E. Miller, Graham R. Mcginnis, Brian Kliszczewicz, Dustin Slivka, Walter Hailes, John Cuddy, Charles Dumke, Brent Ruby, John C. Quindry

Integrative Physiology and Athletic Training Faculty Publications

Oxidative stress occurs as a result of altitude-induced hypobaric hypoxia and physical exercise. The effect of exercise on oxidative stress under hypobaric hypoxia is not well understood. Purpose: To determine the effect of high-altitude exercise on blood oxidative stress. Nine male participants completed a 2-d trek up and down Mt Rainer, in North America, at a peak altitude of 4,393 m. Day 1 consisted of steady-pace climbing for 6.25 hr to a final elevation of 3,000 m. The 4,393-m summit was reached on Day 2 in approximately 5 hr. Climb rest intervals varied but were consistent between participants, with approximately …

The Β-Cyanoalanine Pathway Is Involved In The Response To Water Deficit In Arabidopsis Thaliana., Marylou Machingura, Aissatou Sidibe, Andrew J Wood, Stephen Ebbs

Publications

The β-cyanoalanine pathway is primarily responsible for detoxification of excess cyanide produced by plants. Recent evidence suggests that cyanide detoxification via this pathway may be involved in the response and tolerance to water deficit in plants. The aim of this study was to explore this role in Arabidopsis thaliana in greater detail. The first objective was to establish responsiveness of the pathway to the magnitude and duration of water deficit. The second objective was to examine how interruption of single genes (AtCysA1, AtCysC1 and AtNIT4) encoding enzymes of the pathway influenced the ability to metabolize cyanide and withstand water deficit. …

Graduate News, Office Of Graduate Studies, Old Dominion University

Graduate News

February 2013 issue of Graduate News from the Office of Graduate Studies (now, ODU Graduate School).

Landscape, History And The Media: An Introduction, Christina E. Dando

Geography and Geology Faculty Publications

Writing from Nebraska’s eastern edge, my mind’s eye drawn to the Platte River (west and south of me), I consider landscape, history, and the media. The Oto called the river “Nebraskier” which means flat or shallow, giving us the name of the state.1 Early accounts describe the Platte as “a mile wide and an inch deep” and “too thick to drink, too thin to plow”; Washington Irving described it as “the most magnificent and useless of rivers” (Allin 1982, 1). But to dismiss this river is to judge too quickly. As the river gains momentum, growing in size, it is …

S-Adenosyl-L-Methionine Protects The Probiotic Yest, Saccharomyces Boulardii, From Acid-Induced Cell Death, Nicanor Austriaco, Vincent Cascio, Daniel Gittings, Kristen Merloni, David Laprade

Biology Faculty Publications

Background

Saccharomyces boulardii is a probiotic yeast routinely used to prevent and to treat gastrointestinal disorders, including the antibiotic-associated diarrhea caused by Clostridium difficile infections. However, only 1-3% of the yeast administered orally is recovered alive in the feces suggesting that this yeast is unable to survive the acidic environment of the gastrointestinal tract.

Results

We provide evidence that suggests that S. boulardii undergoes programmed cell death (PCD) in acidic environments, which is accompanied by the generation of reactive oxygen species and the appearance of caspase-like activity. To better understand the mechanism of cell death at the molecular level, we …

A Tale Of Two Compadres: Teaching International Trade And Development Across Cultures, Marjorie Florestal

Global Business & Development Law Journal

No abstract provided.

Honokiol Blocks Store Operated Calcium Entry In Cho Cells Expressing The M3 Muscarinic Receptor: Honokiol And Muscarinic Signaling, Hsiu-Jen Wang, Alexis Martin, Po-Kuan Chao, Rhett A. Reichard, Adam L. Martin, Yue-Wern Huang, Ming-Huan Chan, Robert Aronstam

Biological Sciences Faculty Research & Creative Works

Background: Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees and present in Asian herbal teas, has a unique array of pharmacological actions, including the inhibition of multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol; cytotoxicity was determined using a dye reduction assay.

Results: Honokiol had a potent (EC50 ≈ 5 μmol/l) inhibitory effect on store …

Poised To Prosper? A Cross-System Comparison Of Climate Change Effects On Native And Non-Native Species Performance, Cascade J. B. Sorte, Ines Ibáñez, Dana M. Blumenthal, Nicole A. Molinari, Luke P. Miller, Edwin D. Grosholz, Jeffrey M. Diez, Carla M. D'Antonio, Julian D. Olden, Sierra J. Jones, Jeffrey S. Dukes

Faculty Publications, Biological Sciences

Climate change and biological invasions are primary threats to global biodiversity that may interact in the future. To date, the hypothesis that climate change will favour non-native species has been examined exclusively through local comparisons of single or few species. Here, we take a meta-analytical approach to broadly evaluate whether non-native species are poised to respond more positively than native species to future climatic conditions. We compiled a database of studies in aquatic and terrestrial ecosystems that reported performance measures of non-native (157 species) and co-occurring native species (204 species) under different temperature, CO2 and precipitation conditions. Our analyses revealed …

A Socratic Method For Surveying Students’ Readiness To Study Evolution, William D. Stansfield

Biological Sciences

Before beginning a series of presentations on evolution, it would be prudent to survey the general level of students’ understanding of prerequisite basic concepts of reproduction, heredity, ontology, and phenotypic diversity so that teachers can avoid devoting time to well-known subjects of general knowledge and can spend more time on subjects that are unknown, forgotten, or misunderstood by most students. This article outlines a Socratic method for surveying and teaching to address these concerns.

Research Challenges And Opportunities In Knowledge Representation, Natasha Noy, Deborah Mcguinness, Eyal Amir, Chitta Baral, Michael Beetz, Sean Bechhofer, Craig Boutilier, Anthony Cohn, Johan De Kleer, Michel Dumontier, Tim Finin, Kenneth Forbus, Lise Getoor, Yolanda Gil, Jeff Heflin, Pascal Hitzler, Craig Knoblock, Henry Kautz, Yuliya Lierler, Vladimir Lifschitz, Peter F. Patel-Schneider, Christine Piatko, Doug Riecken, Mark Schildhauer

Computer Science and Engineering Faculty Publications

Modern intelligent systems in every area of science rely critically on knowledge representation and reasoning (KR). The techniques and methods developed by the researchers in knowledge representation and reasoning are key drivers of innovation in computer science; they have led to significant advances in practical applications in a wide range of areas from natural-­‐language processing to robotics to software engineering. Emerging fields such as the semantic web, computational biology, social computing, and many others rely on and contribute to advances in knowledge representation. As the era of “Big Data” evolves, scientists in a broad range of disciplines are increasingly relying …

Prism #69, February 2013, Office Of The Provost

Prism: Western Michigan University's Newsletter for Academic Affairs

No abstract provided.

Bridges : Newsletter Of The Stony Brook Health Sciences Chapter Of United University Professions, Stony Brook University

Bridges Newsletter (UUP HSC Chapter)

Newsletter

Influence Of Upper-Body Exercise Order On Hormonal Responses In Trained Men, Roberto Simao, Richard Diego Leite, Guilherme Fleury Fina Speretta, Alex Souto Maior, Belmiro Freitas De Salles, Tacito Pessoa De Souza Jr, Jakob L. Vingren, Jeffrey Willardson

Faculty Research and Creative Activity

The aim of this study was to examine acute hormonal responses after different sequences of an upper-body resistance-exercise session. Twenty men completed 2 sessions (3 sets; 70% 1-repetition maximum; 2 min passive rest between sets) of the same exercises in opposite sequences (larger to smaller vs. smaller to larger muscle-group exercises). Total testosterone (TT), free testosterone (FT), testosterone/cortisol (T/C) ratio, sex-hormone-binding globulin (SHBG), growth hormone (GH), and cortisol (C) concentrations were measured before and immediately after each sequence. The results indicate that the GH concentration increased after both sessions, but the increase was significantly greater (p < 0.05) after the sequence in which larger muscle-group exercises were performed prior to the smaller muscle-group exercises. No differences were observed between sessions for TT, FT, SHBG, C, or the T/C ratio at baseline or immediately after resistance exercise. These results indicate that performing larger muscle-group exercises first in an upper-body resistance-exercise session leads to a significantly greater GH response. This may have been due to the significantly greater exercise volume accomplished. In summary, the findings of this investigation support the common prescriptive recommendation to perform larger-muscle group exercises first during a resistance-exercise session.

A Rational Free Energy-Based Approach To Understanding And Targeting Disease-Causing Missense Mutations., Zhe Zhang, Shawn Witham, Marharita Petukh, Gautier Moroy, Maria Miteva, Yoshihiko Ikeguchi, Emil Alexov

Publications

BACKGROUND AND SIGNIFICANCE:

Intellectual disability is a condition characterized by significant limitations in cognitive abilities and social/behavioral adaptive skills and is an important reason for pediatric, neurologic, and genetic referrals. Approximately 10% of protein-encoding genes on the X chromosome are implicated in intellectual disability, and the corresponding intellectual disability is termed X-linked ID (XLID). Although few mutations and a small number of families have been identified and XLID is rare, collectively the impact of XLID is significant because patients usually are unable to fully participate in society.

OBJECTIVE:

To reveal the molecular mechanisms of various intellectual disabilities and to suggest …