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2013

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Nutrient Limitation Of Bioluminescent Dinoflagellates In Mangrove Lagoon, Salt River Bay, St. Croix, Usvi, Michelle Zimberlin Jan 2013

Nutrient Limitation Of Bioluminescent Dinoflagellates In Mangrove Lagoon, Salt River Bay, St. Croix, Usvi, Michelle Zimberlin

Theses and Dissertations

Bioluminescent bays resulting from dense concentrations of bioluminescent dinoflagellates are rare, with only 14 documented systems worldwide. One of these bays, Mangrove Lagoon, is located in Salt River Bay National Park in St. Croix, USVI. Very little is known about the environmental factors responsible for maintaining the high dinoflagellate densities in this lagoon. In order to assess the dynamics of the dinoflagellates, in situ nutrient addition bioassays were conducted to determine which nutrients regulate the phytoplankton community, which includes the bioluminescent dinoflagellate Pyrodinium bahamense. Bioassays were conducted in two different seasons, (January 2013 and May 2013) to examine seasonal responses …

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Uncovering The Structures In Ecological Networks: Multiple Techniques For Multiple Purposes, Peng Gao Jan 2013

Uncovering The Structures In Ecological Networks: Multiple Techniques For Multiple Purposes, Peng Gao

Theses and Dissertations

Ecosystem structure and function are the product of biological and ecological elements and their connections and interactions. Understanding structure and process in ecosystems is critical to ecological studies. Ecological networks, based on simple concepts in which biological and ecological elements are depicted as nodes with relationships between them described as links, have been recognized as a valuable means of clarifying the relationship between structures and process in ecosystems. Ecological network analysis has benefited from the advancement of techniques in social science, computer science, and mathematics, but attention must be paid to whether the designs of these techniques follow ecological principles …

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Understanding The Early Stages Of Development Of A Global Health Partnership, Wendy Raquel Gonzalez Navarrete Jan 2013

Understanding The Early Stages Of Development Of A Global Health Partnership, Wendy Raquel Gonzalez Navarrete

Theses and Dissertations

Maternal and child undernutrition contributes to more than one-third of child deaths. Global Health Partnerships (GHP) have emerged as a response to undernutrition and other pressing health problems. GHPs promote joint decision-making among donors, multilateral agencies, and country partners. Despite their positive impact on health problems, GHPs have generated unintended negative effects on country partners. This study aimed to understand the factors, strategies, and processes conducive to the establishment of an effective GHP in the context of a cooperative regional effort to reduce undernutrition and improve maternal and child health in eight countries in Latin America, Regional Health Initiative (RHI). …

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Active 6 After-School Physical Activity Program: A Process Evaluation, William David Reamer Jan 2013

Active 6 After-School Physical Activity Program: A Process Evaluation, William David Reamer

Graduate Student Theses, Dissertations, & Professional Papers

The purpose of this study was to identify barriers to participation in the Active 6 After-School Physical Activity (A6) program in Missoula, MT. Barriers were explored by identifying factors that influence participation in after-school programs. Those barriers identified in the literature were compared with current needs and existing resources of the target population. To evaluate the barriers to the A6 program, primary qualitative data was collected from two sets of three focus groups; one high socio-economic status (SES) and one low. The results of this assessment identified barriers to participation in the A6 program from both parents/guardians and kids. Barriers …

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A Bitter Policy Shoved Down Our Throats: How A Once Admirable And Necessary Agricultural Program Has Resulted In Major Profits For Big Business And Major Frustration For Others., David Ryan Quintanilla Jan 2013

A Bitter Policy Shoved Down Our Throats: How A Once Admirable And Necessary Agricultural Program Has Resulted In Major Profits For Big Business And Major Frustration For Others., David Ryan Quintanilla

The Scholar: St. Mary's Law Review on Race and Social Justice

Abstract Forthcoming.

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Cultivation Of Auricularia Polytricha Mont. Sacc (Black Jelly Mushroom) Using Oil Palm Wastes / Dang Lelamurni Abd. Razak, Dang Lelamurni Abd. Razak Jan 2013

Cultivation Of Auricularia Polytricha Mont. Sacc (Black Jelly Mushroom) Using Oil Palm Wastes / Dang Lelamurni Abd. Razak, Dang Lelamurni Abd. Razak

Student Works (2010-2019)

The increasing demand for continuous and quality supply of many types of mushrooms has drawn much attention and research to find potential substrate and domesticating the cultivation several species of edible mushrooms to suit local environmental conditions and resources. One of the popular mushrooms among Malaysians due to its nutritional and medicinal values, Auricularia polytricha or Black Jelly, offers high income to local growers due to its ability to grow and fruit in tropical region such as Malaysia. The availability of a wide variety of agro-industrial byproducts rich in lignocellulose and other materials required for mushroom growth should be explored …

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Peptides Inhibiting Angiotensin I-Converting Enzyme Derived From Edible Mushrooms / Lau Ching Ching, Ching Ching Lau Jan 2013

Peptides Inhibiting Angiotensin I-Converting Enzyme Derived From Edible Mushrooms / Lau Ching Ching, Ching Ching Lau

Student Works (2010-2019)

Hypertension is one of the major health problems worldwide. Bioactive peptides that inhibit angiotensin I-converting enzyme (ACE) in the blood pressure regulation system (renin-angiotensin system) can contribute to the prevention and treatment of hypertension. Mushroom species studied are high in protein content, which makes them potentially a good source of antihypertensive peptides. Among the nine edible mushrooms evaluated, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) exhibited high levels of ACE inhibitory activity. The protein extracts were fractionated by reverse-phase high performance liquid chromatography (RPHPLC) into six fractions each. Fraction 3 from E5Pc (E5PcF3) …

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Production Of Goat Offspring From In Vivo-Derived Embryos Through Embryo Transfer Technique / Xiao Zhi Chao, Zhi Chao Xiao Jan 2013

Production Of Goat Offspring From In Vivo-Derived Embryos Through Embryo Transfer Technique / Xiao Zhi Chao, Zhi Chao Xiao

Student Works (2010-2019)

Goat emAbryo transfer is an important tool to improve the genetics and to increase rapidly the number of economically superior goat for the industry. The objective of this study was to develop a suitable protocol for the collection and transfer of goat embryos as well as subsequently to produce goat kids through embryo transfer. Two different hormones (PMSG, FSH-V), two different localities (Kuala Lumpur, Malaysia and Kunming, China) and the embryo recovery cycle were designed to evaluate the ovarian responses and embryo transfer performance. For donor goats, the treatment was 14 or 17 days CIDR and before CIDR withdrawal, 1200 …

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Enzymatic Assisted Aqueous Oil Extraction From Jatropha Curcas / Hazrati Wazir, Hazrati Wazir Jan 2013

Enzymatic Assisted Aqueous Oil Extraction From Jatropha Curcas / Hazrati Wazir, Hazrati Wazir

Student Works (2010-2019)

Enzymatic assisted aqueous oil extraction (EAAOE) of Jatropha curcas was investigated in this research. The presence of Alcalase® 2.4 L and Celluclast® 1.5 L facilitates the recovery of the oil up to 58%. Parameters involved in EAAOE such as selection and concentration of enzymes, ratio of Jatropha curcas seeds: water (mass/volume), pH, temperature, duration of incubation time, effect of ultrasonication, and oil recovery from oil-water mixture were optimised. The highest oil recovery (58%, m/m) was obtained with 6 mL/1g water/oil, 6% (m/m) enzyme, temperature at 50°C, seed particle size less than 1 mm, pH 5.0 and pH 8.0 for Celluclast® …

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Ecology Of Egrets (Ardeidae) At The Palm Oil Mill Effluent Ponds In Carey Island, Selangor, Peninsular Malaysia / Abdoul Baset Hassen Aboushiba, Abdoul Baset Hassen Aboushiba Jan 2013

Ecology Of Egrets (Ardeidae) At The Palm Oil Mill Effluent Ponds In Carey Island, Selangor, Peninsular Malaysia / Abdoul Baset Hassen Aboushiba, Abdoul Baset Hassen Aboushiba

Student Works (2010-2019)

Egrets (Aves: Ardeidae) are gregarious and cosmopolitan wading birds, widely distributed throughout the world and associated with wetland habitat particularly with shallow water covered with short vegetation or without vegetation for foraging. A wetland habitats are facing overwhelming pressure due to anthropogenic activities such as urbanization and conversion into agricultural fields which causes habitat loss and degradation that ultimately affects the population of different egret species. Egrets employ different foraging behaviours to exploit the wide range of prey items for their survival and reproduction. Study on egrets’ relative abundance, foraging strategies, food diversity, and its relationships to the quality of …

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Antioxidant And Insulin-Like Properties Of Extracts From Wheat Grains Fermented By Selected Indigenous Ganoderma Spp. / Sarasvathy Subramaniam, Sarasvathy Subramaniam Jan 2013

Antioxidant And Insulin-Like Properties Of Extracts From Wheat Grains Fermented By Selected Indigenous Ganoderma Spp. / Sarasvathy Subramaniam, Sarasvathy Subramaniam

Student Works (2010-2019)

Ganoderma are cosmopolitan wood decaying white rot basidiomycete fungi,which have been used for centuries for medicinal purposes, particularly in China, Japan and Korea. It is believed to possess many health benefits and has been reported to be effective against various diseases by virtue of their antioxidant potential. In the present study, solid-substrate fermentation of wheat grains with indigenous Ganoderma spp. selected based on ethnomycological knowledge was carried out. The species included Ganoderma australe (Fr.) Pat. (KUM60813), Ganoderma neo-japonicum Imazeki(KUM61076) and Ganoderma lucidum (Fr.) Karst. (VITA GL) (a commercial strain). Antioxidant activities of the crude aqueous and ethanol extracts of the …

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Wastewater Treatment Using Microalgae / Azna Binti Abdul Aziz, Azna Abdul Aziz Jan 2013

Wastewater Treatment Using Microalgae / Azna Binti Abdul Aziz, Azna Abdul Aziz

Student Works (2010-2019)

Three species of selected microalgae isolated from Tasik Taman Jaya, Oscillatoria limnosa, Scenedesmus quadricauda and Pediastrum duplex, were cultured in Chu no. 10 defined medium. After the microalgae reached their optimum growth they were grown in wastewater to observe their capability to remove inorganic nutrients in wastewater. The microalgae showed steady increase in cell density for the duration of laboratory observation of eighteen days. As the cell density of the microalgae increased, both nitrate and phosphate concentration in wastewater decreased. This study showed that all the three species of microalgae have the ability to reduce the nutrients. Among them, Pediastrum …

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An Investigation Of The Roles Of Myb Transcription Factors In Orchid Dendrobium Hybrida Flower Development / Lau Su Ee, Su Ee Lau Jan 2013

An Investigation Of The Roles Of Myb Transcription Factors In Orchid Dendrobium Hybrida Flower Development / Lau Su Ee, Su Ee Lau

Student Works (2010-2019)

MYB genes encode a large family of transcription regulators in eukaryotes. Most plant MYB genes belong to the R2R3-MYB subfamily. MYB gene family members have been studied in various plant species and play roles in the regulation of plant development, hormone signalling, defence response and secondary metabolism. Orchid plants play an important role in the floral industry owing to their unique flower shapes, fragrance, colours and keeping quality. Flower colours are influenced by flower pigmentation, vacuolar compounds, pH, metal ions and shape of epidermal cells. R2R3-MYB genes regulate flower pigmentation, flower shape and cell shape therefore have importance in the …

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Isolation And Characterization Of Angiotensin Converting Enzyme (Ace) Inhibitory Compounds And Antioxidant Activities Of Tacca Integrifolia / Farah Liyana Binti Jamaludin, Farah Liyana Jamaludin Jan 2013

Isolation And Characterization Of Angiotensin Converting Enzyme (Ace) Inhibitory Compounds And Antioxidant Activities Of Tacca Integrifolia / Farah Liyana Binti Jamaludin, Farah Liyana Jamaludin

Student Works (2010-2019)

Tacca integrifolia Ker-Gawl is belongs to the family of Taccaceae and locally know as “Belimbing Tanah”. It has been used traditionally for the treatment of hypertension, hemorrhoids, heart failure and kidney disease. The thin layer chromatography (TLC) of hexane, petroleum ether, chloroform, methanol and water extract of leaves and rhizome showed the presence of phenols, flavonoids, terpenoids, essential oil and alkaloid compounds. The analysis of the leaves and rhizome extract with LCMS/MS showed that it contained proanthocyanidin, proanthocyanidin trimer, p-hydroxybenzoic acid, phenolic acid conjugate, protocatechuic acid, quinic acid and dicaffeolquinic acid conjugate. The total phenol contents w e re highest …

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Selected Malaysian Honey And Its Efficacy Towards Excisional Wound Healing / Tan Mui Koon, Mui Koon Tan Jan 2013

Selected Malaysian Honey And Its Efficacy Towards Excisional Wound Healing / Tan Mui Koon, Mui Koon Tan

Student Works (2010-2019)

Skin is involved in protection, sensory transduction, thermoregulation and metabolic functions. Thus, wound on the skin needs to be effectively addressed and treated. Wound healing process is a complex, systematic and overlapping process that leads to restoration of tissue integrity and also its functions. This complicated wound healing process involves several phases including inflammatory phase, proliferative phase and remodelling phase. Fundamental to wound healing research is the search for best wound healing experimental model and for the substance that best treats wounds. Honey, which in natural state is mildly acidic, is one of the potential candidates for wound treatment. It …

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Isolation And Characterization Of Indigenous Microorganisms From Agricultural Soil / Mahsa Ansari, Mahsa Ansari Jan 2013

Isolation And Characterization Of Indigenous Microorganisms From Agricultural Soil / Mahsa Ansari, Mahsa Ansari

Student Works (2010-2019)

Microorganisms have many properties include the ability to degrade waste products. In this study Haemolysis was used as an initial selection criterion for the primary isolation of surfactant-producing bacteria. Only β and γ Haemolytic activity were detected in this study which as the presence of a definite clear zone around a colony. From this study, Out of 139 (49 %) hemolytic colonies were observed in this study had shown strong haemolytic activity. The Gram stain results had showed: cocci and bacilli shapes. The colony morphology identification had shown rough, smooth and mucoid appearance. 120 of 139 (86%) isolates possessed biocleaner …

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Evaluation Of Potential Role Of Catharanthus Roseus And Acanthus Ilicifolius In The Prevention Of Azoxymethane-Induced Aberrant Crypt Foci In Rat's Colon / Amel Abdullah Almagrami, Amel Abdullah Almagrami Jan 2013

Evaluation Of Potential Role Of Catharanthus Roseus And Acanthus Ilicifolius In The Prevention Of Azoxymethane-Induced Aberrant Crypt Foci In Rat's Colon / Amel Abdullah Almagrami, Amel Abdullah Almagrami

Student Works (2010-2019)

Catharanthus roseus extract is traditionally used to treat different diseases including cancer in Southeast Asia. Acanthus ilicifolius, is a mangrove medicinal plant, and is widely used by the local inhabitants of the Sundarbans (India) to treat a variety of diseases. The aim of current research is to assess the chemoprotective outcomes of ethanolic extracts of C. roseus and A. ilicifolius against azoxymethane (AOM) induced colonic aberrant crypt foci (ACF) in rats. The bioactivities of the crude ethanol extracts of C. roseus and A. ilicifolius were investigated in both of their in vitro antioxidant and in vivo oral toxicity tests. Antioxidant …

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Molecular Epidemiology Of Blastocystis Isolated From Malaysia And Libya / Awatif Mohamed Abdulsalam, Abdulsalam Awatif Mohamed Jan 2013

Molecular Epidemiology Of Blastocystis Isolated From Malaysia And Libya / Awatif Mohamed Abdulsalam, Abdulsalam Awatif Mohamed

Student Works (2010-2019)

Blastocystis is a common intestinal parasite infecting humans and different animal species. The purpose of this study was to investigate the molecular epidemiology of Blastocystis infection in Malaysia and Libya, and to investigate the occurrence of Blastocystis sp. in water sources from Malaysia. Two groups of stool samples were collected from 300 primary schoolchildren in Pahang, Malaysia and 380 outpatients attending the Central Laboratory in Sebha, Libya. While for water, a total of 65 samples were collected from different water sources in the study areas of Pahang, Malaysia. The stools and water samples were processed accordingly and were subjected to …

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The Implementation Of Esl Curriculum Policy And Its Impact On Practice In Selected Primary Schools Of Madurai District, India / Latha D/O Ravindran, Latha Ravindran Jan 2013

The Implementation Of Esl Curriculum Policy And Its Impact On Practice In Selected Primary Schools Of Madurai District, India / Latha D/O Ravindran, Latha Ravindran

Student Works (2010-2019)

This study is driven by the need to examine the implementation of ESL curriculum policy and its implications for practice. Implementation of the ESL policy is an evolutionary process which is not always linear or predictable. As policy is transformed and adapted to conditions of the implementing unit, effective implementation may have different meanings in different settings. Gaps between policy planning and implementation often exist, despite careful planning of the educational policy. Drawing on a qualitative design, various approaches such as interviews with the key stakeholders, interviews with students, document analysis, field notes and classroom observations helped the researcher to …

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A Qualitative Research On Patient Care Management In Malaysian Hospitals; Patients And Doctors Experiences Of Bedside Care / Susan Thomas, Thomas Susan Jan 2013

A Qualitative Research On Patient Care Management In Malaysian Hospitals; Patients And Doctors Experiences Of Bedside Care / Susan Thomas, Thomas Susan

Student Works (2010-2019)

Over the past decade there has been an exponential increase of tertiary level specialized care to meet the needs of the population in Malaysia. The organisation structure of MOH which encompasses the three levels of the health care delivery system, which are Federal, State and District levels, demonstrates decentralization and efficiency. This system is changing towards wellness service that emphasizes a lifetime health plan in keeping the child and family well. For centuries the traditional practice demonstrated by doctors has been a system of inquiry which consists of close-ended questions that fulfil the doctors’ need in collecting information. This process …

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Efficacy Of Switching To Aripiprazole And Ziprasidone On Clinical And Metabolic Profile Among Schizophrenic Patients With Metabolic Syndrome / Mas Ayu Binti Said, Said Mas Ayu Jan 2013

Efficacy Of Switching To Aripiprazole And Ziprasidone On Clinical And Metabolic Profile Among Schizophrenic Patients With Metabolic Syndrome / Mas Ayu Binti Said, Said Mas Ayu

Student Works (2010-2019)

Introduction: Schizophrenia is a devastating mental illness that impairs mental and social functioning and often leads to the development of co-morbid diseases. They are at greater risk for metabolic dysfunctions than other individuals due to a number of reasons, including inactive lifestyle, poor dietary choices, and side effects of antipsychotic medications. Atypical antipsychotics were reported to be associated with increased risk of hyperglycaemia and hyperlipidemia, and subsequently increase the risk of metabolic syndrome. However, ziprasidone and aripiprazole have a favourable metabolic profile. Objectives: i) To determine the prevalence of metabolic syndrome and its components among schizophrenia patients. ii) To determine …

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Phyllanthus Inhibits Proliferation, Metastasis, Angiogenesis And Induces Apoptosis Of Human Melanoma (Mewo) And Prostate Adenocarcinoma (Pc-3) Cells Through Modulation Of Multiple Cell Signalling Pathways / Tang Yin Quan, Yin Quan Tang Jan 2013

Phyllanthus Inhibits Proliferation, Metastasis, Angiogenesis And Induces Apoptosis Of Human Melanoma (Mewo) And Prostate Adenocarcinoma (Pc-3) Cells Through Modulation Of Multiple Cell Signalling Pathways / Tang Yin Quan, Yin Quan Tang

Student Works (2010-2019)

Modern cancer treatment therapies such as surgery, chemotherapy and immunotherapy are deemed relatively unsuccessful due to their ineffectiveness, safety issues and costliness. As not all cancer patients respond positively to current anticancer agents, mortality rates of cancer are on a continuous rise including melanoma and prostate cancer. Natural product-based traditional medicine, often overshadowed by modern medicine, has returned to be a holistic approach for health care in many societies. This “back to basic” approach is due to its long history of usage in disease treatment and the pharmacological/nutritional value of these products which are believed to be able to halt/delay …

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Evaluation Of Hepatoprotective Effects And Bioactivities Of Phyllanthus Niruri And Melastoma Malabathricum / Zahra Abdulqader Amin, Abdulqader Amin Zahra Jan 2013

Evaluation Of Hepatoprotective Effects And Bioactivities Of Phyllanthus Niruri And Melastoma Malabathricum / Zahra Abdulqader Amin, Abdulqader Amin Zahra

Student Works (2010-2019)

The liver plays an essential role in the body by regulating several important metabolic functions. Liver injury is associated with distortion of these functions causing many health problems. Pharmaceutical drugs treat liver disorders but carry the risk of causing further damage to the liver. Hence, herbal drugs have been becoming increasingly popular worldwide as both patients and doctors strive to find safer alternatives. The bioactivities of the crude ethanol extracts of Phyllnthus niruri L. (PN) and Melastoma malabathricum L. (MM) were investigated in virtue of their in vitro antioxidant, immunomodulatory effects and in vivo oral toxicity tests. The hepatoprotective activity …

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Mechanisms Of Wound Healing And Gastro Protective Effects Of Ethanol Leaf Extract Of Jasminum Sambac And Hemigraphis Colorata On Hcl/Ethanol-Induced Gastric Injury In Experimental Animals / Ahmed Salim Ahmed Al Rashdi, Ahmed Al Rashdi Ahmed Salim Jan 2013

Mechanisms Of Wound Healing And Gastro Protective Effects Of Ethanol Leaf Extract Of Jasminum Sambac And Hemigraphis Colorata On Hcl/Ethanol-Induced Gastric Injury In Experimental Animals / Ahmed Salim Ahmed Al Rashdi, Ahmed Al Rashdi Ahmed Salim

Student Works (2010-2019)

Sprague Dawley rats weighing 225-250 g were used for evaluating wound healing and gastroprotective activities. The plant extracts of J.sambac and H.colorata were assessed in-vivo for toxicity study. Antioxidant properties of the crude extracts were determined using DPPH radical scavenging assay and total phenolic content. Β-sitosterol and octyl gallate compounds were isolated from J. sambac and H. Colorata petroleum ether crude extracts by CC, TLC and HPLC and were evaluated for their gastroprotective activities.Macroscopic finding showed that H.colorata and J.sambac significantly accelerated wound healing compared to vehicle. Post wounding tissues collected on day 10th for oxidative damage evaluation revealed significant …

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Development Of A Novel Diagnostic Technique And Geospatial Database For Soil-Transmitted Helminthiasis In Malaysia / Romano Ngui, Ngui Romano Jan 2013

Development Of A Novel Diagnostic Technique And Geospatial Database For Soil-Transmitted Helminthiasis In Malaysia / Romano Ngui, Ngui Romano

Student Works (2010-2019)

The main objectives of this thesis are two pronged which include the development of a novel diagnostic technique and the creation a geospatial database for soil-transmitted helminthiasis in Malaysia. These aims have been successfully accomplished and the outcomes are detailed out in Chapters 5 and 7. Nonetheless in order to achieve these good outcomes, prior work in getting appropriate samples for high resolution melting (HRM) analysis and current data for geographical information system (GIS) had to be conducted in some epidemiological studies in the human and animal populations and these are illustrated in Chapters 3, 4 and 6. Therefore, this …

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Experience Of Patients And Carers With Traditional Healing And Psychiatric Services, And The Outcome Of Psychosis For Patients Who Attend Traditional Services In Sudan / Ehab Ali Sorketti Koriana, Ehab Ali Sorketti Koriana Jan 2013

Experience Of Patients And Carers With Traditional Healing And Psychiatric Services, And The Outcome Of Psychosis For Patients Who Attend Traditional Services In Sudan / Ehab Ali Sorketti Koriana, Ehab Ali Sorketti Koriana

Student Works (2010-2019)

The use of traditional healers is common worldwide, especially in developing countries. Few studies, though, have focused on the use of traditional healers by people with mental disorders. This study made an attempt to understand the practice of traditional healers in relation to mental health and psychiatric services in Sudan. The study was framed to understand: (a) the socio-demographic characteristics of people with mental disorders who seek treatment in the traditional healer centres in central Sudan; (b) the clinical presentations, diagnoses and outcomes of people with psychotic disorders attending traditional healers, as well as the healing methods and interventions used; …

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Determination Of Multiclass Pesticide Residues In Fruits And Vegetables With Headspace Solid Phase Microextraction Gas Chromatography Mass Spectrometry Using Experimental Factorial Design / Abdulra’Uf Lukman Bola, Abdulra’Uf Lukman Bola Jan 2013

Determination Of Multiclass Pesticide Residues In Fruits And Vegetables With Headspace Solid Phase Microextraction Gas Chromatography Mass Spectrometry Using Experimental Factorial Design / Abdulra’Uf Lukman Bola, Abdulra’Uf Lukman Bola

Student Works (2010-2019)

The focus of this study is the development and validation of headspace solid phase microextraction and chromatographic techniques for the analysis of pesticide residues in fruit and vegetable samples. The developed method is based on the comparison of the optimized HS-SPME parameters using univariate and multivariate experimental designs. Modern trends in sample preparation and subsequent instrumental analysis are aimed towards reliable and accurate analysis of pesticide residues and other contaminants from food. It is focused on the simplifications, miniaturization, improvement and reduction in volume of sample and solvents used while maintaining high throughput performance. The microextraction techniques have been developed …

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Investigating Gdf5-Induced Tenogenesis In Mesenchymal Stem Cells For The Potential Improvement Of Tendon Repair / Tan Sik Loo, Sik Loo Tan Jan 2013

Investigating Gdf5-Induced Tenogenesis In Mesenchymal Stem Cells For The Potential Improvement Of Tendon Repair / Tan Sik Loo, Sik Loo Tan

Student Works (2010-2019)

Mesenchymal stem cell (MSC) has an immense potential for use in clinical applications, however, there is still paucity in the fundamental knowledge in the techniques and processes involved in MSCs differentiation, especially tenogenic differentiation. Growth and differentiation factor 5 (GDF5) is a potential inducer which may induce tenogenic differentiation response in MSC. The aims of this current study were: (1) to investigate the tenogenic differentiation in human and rabbit MSCs (hMSCs and rbMSCs respectively) in response to GDF5 treatment; (2) to investigate the potential of GDF5-induced rbMSCs in tendon repair in in vivo rabbit model; and (3) to investigate the …

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Antioxidant And Cytotoxicity Activities Of Veitchia Merrillii Fruits / Ali Vafaei, Ali Vafaei Jan 2013

Antioxidant And Cytotoxicity Activities Of Veitchia Merrillii Fruits / Ali Vafaei, Ali Vafaei

Student Works (2010-2019)

Veitchia merrillii (Arecaceae family) is commonly known as the "Christmas Palm" because its fruits become bright scarlet and tend to be that color in winter. A study was conducted to evaluate Veitchia merrillii fruits for the presence of total phenolic and flavonoid contents and determine antioxidant activity as well as cytotoxicity effects of extracts with solvents methanol, ethyl acetate and water. Further more, qualitative and quantitative composition of phenolics and flavonoid compounds in all extracts were also analyzed using RP-HPLC. The results of the study showed that methanol extract gave the highest yield compared to the other solvents used. The …

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Determination Of Hydroxyproline In Kalanchoe Pinnata By Hplc / Nurul Nadia Binti Zainal Abidin, Nurul Nadia Zainal Abidin Jan 2013

Determination Of Hydroxyproline In Kalanchoe Pinnata By Hplc / Nurul Nadia Binti Zainal Abidin, Nurul Nadia Zainal Abidin

Student Works (2010-2019)

Kalanchoe pinnata is an important plant which has many traditional medicinal uses. In this work, hydroxyproline was extracted from Kalanchoe pinnata. Determination of hydroxyproline in this plant was done using HPLC. Hydroxyproline is one of the element for the formation of collagen. There are three solvent composition used for extraction; 100% of water, 100% of ethanol and 50% of ethanol. It was found that combination of two solvent, ethanol and water can optimize the extraction as when comparing the concentration of hydroxyproline detected in the same extraction time, this system showed the highest concentration of hydroxyproline detected. Another parameter is …

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