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2013

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Antioxidant And Cytotoxicity Activities Of Veitchia Merrillii Fruits / Ali Vafaei, Ali Vafaei Jan 2013

Antioxidant And Cytotoxicity Activities Of Veitchia Merrillii Fruits / Ali Vafaei, Ali Vafaei

Student Works (2010-2019)

Veitchia merrillii (Arecaceae family) is commonly known as the "Christmas Palm" because its fruits become bright scarlet and tend to be that color in winter. A study was conducted to evaluate Veitchia merrillii fruits for the presence of total phenolic and flavonoid contents and determine antioxidant activity as well as cytotoxicity effects of extracts with solvents methanol, ethyl acetate and water. Further more, qualitative and quantitative composition of phenolics and flavonoid compounds in all extracts were also analyzed using RP-HPLC. The results of the study showed that methanol extract gave the highest yield compared to the other solvents used. The …

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Application Of Polypyrrole And Polypyrrole/Polyethylenimine For The Removal Of Some Heavy Metal Ions From Aqueous Solution / Samira Hosseini, Samira Hosseini Jan 2013

Application Of Polypyrrole And Polypyrrole/Polyethylenimine For The Removal Of Some Heavy Metal Ions From Aqueous Solution / Samira Hosseini, Samira Hosseini

Student Works (2010-2019)

Polypyrrole (Ppy) prepared by chemical oxidation using FeCl3 as the oxidant has exhibited 100% sorption capacity for the removal of heavy metal ions (Pb, Ni, Cu, Cd and Zn) from aqueous solutions. Batch adsorption results showed that solution pH had a major impact on metal adsorption. 100% heavy metal absorption was found at pH 7. At both acidic and alkaline solutions beyond pH 7, the adsorption capacity of Ppy was substantially lowered. The effects of other important parameters such as initial concentration of lead stock solution, contact time and the sorbent dosage on the uptake of metallic ions were also …

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Determination Of Hydroxyproline In Kalanchoe Pinnata By Hplc / Nurul Nadia Binti Zainal Abidin, Nurul Nadia Zainal Abidin Jan 2013

Determination Of Hydroxyproline In Kalanchoe Pinnata By Hplc / Nurul Nadia Binti Zainal Abidin, Nurul Nadia Zainal Abidin

Student Works (2010-2019)

Kalanchoe pinnata is an important plant which has many traditional medicinal uses. In this work, hydroxyproline was extracted from Kalanchoe pinnata. Determination of hydroxyproline in this plant was done using HPLC. Hydroxyproline is one of the element for the formation of collagen. There are three solvent composition used for extraction; 100% of water, 100% of ethanol and 50% of ethanol. It was found that combination of two solvent, ethanol and water can optimize the extraction as when comparing the concentration of hydroxyproline detected in the same extraction time, this system showed the highest concentration of hydroxyproline detected. Another parameter is …

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Molecular Strategies For Control Of Cymbidium Mosaic Virus / Adriya Binti Dzulkurnain @ Hamidi, Adriya Dzulkurnain @ Hamidi Jan 2013

Molecular Strategies For Control Of Cymbidium Mosaic Virus / Adriya Binti Dzulkurnain @ Hamidi, Adriya Dzulkurnain @ Hamidi

Student Works (2010-2019)

Orchid, amongst the most popular flowers in the world, play a major role in the economy of many Asian countries. However, detrimental effects by various viruses can reduce the economic value of this horticultural crop. There have been a number of viruses known to occur in orchids globally with one of the most common being Cymbidium mosaic virus (CymMV). Plant genetic engineering approaches have been successful in producing crops with viral resistance, however there has been a concern expressed that resistance resulting from single gene constructs might be rather narrow (specific to only certain virus sub-type) could easily break down. …

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Adsorption Of Parabens In Aqueous Solution Onto Β-Cyclodextrin Cross-Linked Polymer / Chin Yuk Ping, Yuk Ping Chin Jan 2013

Adsorption Of Parabens In Aqueous Solution Onto Β-Cyclodextrin Cross-Linked Polymer / Chin Yuk Ping, Yuk Ping Chin

Student Works (2010-2019)

β-cyclodextrin cross-linked polymers were prepared from hexamethylene diisocyanate (HMDI) and toluene-2,6-diisocyanate (TDI) as a cross-linker. Two types of polymers were synthesized, namely β-CD-HMDI and β-CD-TDI. The polymers were characterized and β-CD-TDI with the molar ratio of 1:7 for β-CD:TDI was chosen for further studies as it gave the highest adsorption of parabens. The adsorption mechanisms were studied with adsorption kinetic models and adsorption isotherm models. Kinetic study was done for single paraben solutions and a mixture of four parabens solution. For the kinetic study, the experimental data were analysed using pseudo-first-order, pseudo-second-order, and intraparticle diffusion kinetic models. Adsorptions follow pseudo-second-order …

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Acid Denaturation Of Aspergillus Niger Glucoamylase And Structural Transitions Induced By Various Alcohols And Polyols / Mohammed Suleiman Mohammed Zaroog, Mohammed Suleiman Mohammed Zaroog Jan 2013

Acid Denaturation Of Aspergillus Niger Glucoamylase And Structural Transitions Induced By Various Alcohols And Polyols / Mohammed Suleiman Mohammed Zaroog, Mohammed Suleiman Mohammed Zaroog

Student Works (2010-2019)

Acid denaturation of Aspergillus niger glucoamylase was studied using different conformational probes. Both far-UV CD spectral signal (MRE222nm) and tryptophan fluorescence remained unchanged in the pH range, 7.0–3.0 but decreased significantly below pH 3.0, whereas the ANS fluorescence showed a marked increase below pH 1.5. Maximal changes in the MRE222nm value and the ANS fluorescence were noticed at pH 1.0. The acid-denatured state of glucoamylase at pH 1.0 retained a significant amount of secondary structure as reflected from the far-UV CD spectra but showed a deformed tertiary structure with significant exposure of the nonpolar groups as well as the tryptophan …

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Overlap Pcr Strategy And Cloning Of Prostate Specific Membrane Antigen (Psma) Gene In Pichia Pastoris Yeast Recombinant Expression System / Nassrin Abduljalil Badroon, Nassrin Abduljalil Badroon Jan 2013

Overlap Pcr Strategy And Cloning Of Prostate Specific Membrane Antigen (Psma) Gene In Pichia Pastoris Yeast Recombinant Expression System / Nassrin Abduljalil Badroon, Nassrin Abduljalil Badroon

Student Works (2010-2019)

In this study, PSMA was divided into 3 fragments, G1, G2 and G3. Two-step PCR was done to constructed G1 sub-divided fragments (a, b, c, d, e, and f). G2 and G3 were amplified using specific primers and the obtained PCR products were subsequently purified and proceed for cloning. In order to clone each PSMA fragment, each PCR amplified fragment were ligated into pGEM-T vector and transformed into JM109 competent cells, which provided the complete sequences of G2 and G3; with some minimum mutations in G1.Overlapping extension PCR was conducted to combine the fragments; so the G1 fragment was amplified …

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Effect Of Superovulation On Ovarian Response And Subsquent Embryo Transfer In Goats / Shariffah Binti Nazari, Shariffah Nazari Jan 2013

Effect Of Superovulation On Ovarian Response And Subsquent Embryo Transfer In Goats / Shariffah Binti Nazari, Shariffah Nazari

Student Works (2010-2019)

This study was conducted to evaluate the effects of oFSH dosages on ovarian responses during superovulation treatment. A total of 35 mature crossbred goats were synchronised for oestrus with CIDR devices for a period of 13 days. The does were divided into 3 groups, namely groups M1, M2 and S receiving 8.8 mg and 14.1 mg of oFSH through multiple intramuscular (i.m.) injection starting from 2 days before the CIDR removal and 8.8 mg of oFSH through single i.m. injection upon CIDR removal, respectively. Ovarian responses of all treatments were evaluated during laparotomy session on days 3 or 7 after …

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Identification, Characterization And Degradation Studies Of Polycyclic Aromatic Hydrocarbons In Recreational Urban Lakes, Road Dusts And Road Side Soils Of Kuala Lumpur City, Malaysia / Nur Aainaa Syafini Binti Mohd Radzi, Nur Aainaa Syafini Mohd Radzi Jan 2013

Identification, Characterization And Degradation Studies Of Polycyclic Aromatic Hydrocarbons In Recreational Urban Lakes, Road Dusts And Road Side Soils Of Kuala Lumpur City, Malaysia / Nur Aainaa Syafini Binti Mohd Radzi, Nur Aainaa Syafini Mohd Radzi

Student Works (2010-2019)

This study is on the distributions and concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) in eleven surface water, suspended particulate matter (SPM) and surface sediments samples collected from two urban recreational lakes namely, Taman Jaya and Lake Perdanas in Kuala Lumpur city. The total PAH concentrations of Lake Taman Jaya and Lake Perdana ranged from 0.56- 1.19 μg L-1 and 0.32- 1.30 μg L-1 in surface water, 0.93- 2.80 μg g-1 and 1.21-2.92 μg g-1 in SPM, and 0.15– 0.23 μg g-1 and 0.01– 0.27 μg g-1 in surface sediments respectively. High molecular weight (HMW) PAHs dominates the distribution of …

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Biological Activities Of Hibiscus Sabdariffa Linn. / Md Hoirul Azri Bin Ponari, Hoirul Azri Ponari Jan 2013

Biological Activities Of Hibiscus Sabdariffa Linn. / Md Hoirul Azri Bin Ponari, Hoirul Azri Ponari

Student Works (2010-2019)

Hibiscus sabdariffa Linn (Roselle) is a member of the Malvaceae family. The origin of H. sabdariffa is not fully known, but in Malaysia it was introduce by University of Malaya in early 1990s and commercially planted by the Department of Agriculture in Rhu Tapai, Terengganu. The present study was designed to investigate the pharmacological activities and phytochemical constituents of three different varieties of H. sabdariffa i.e. Arab, UKMR-1 and UKMR-2 variety. Pharmacological activities studied include determination of antioxidant activities in plant samples using DPPH radical scavenging assay, reducing power assay and metal chelating assay; the toxicity of plant samples in …

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Diversity Of Polyporales In The Malay Peninsular And The Application Of Ganoderma Australe (Fr.) Pat. In Biopulping Of Empty Fruit Bunches Of Elaeis Guineensis / Mohamad Hasnul Bin Bolhassan, Mohamad Hasnul Bolhassan Jan 2013

Diversity Of Polyporales In The Malay Peninsular And The Application Of Ganoderma Australe (Fr.) Pat. In Biopulping Of Empty Fruit Bunches Of Elaeis Guineensis / Mohamad Hasnul Bin Bolhassan, Mohamad Hasnul Bolhassan

Student Works (2010-2019)

Diversity and distribution of Polyporales in Malaysia was investigated by collecting basidiocarps from trunks, branches, exposed roots and soil from six states (Johore, Kedah, Kelantan, Negeri Sembilan, Pahang and Selangor) in Peninsular Malaysia and Federal Territory Kuala Lumpur. The morphological study of 99 basidiomata collected from 2006 till 2007 and 241 herbarium specimens collected from 2003 - 2005 were undertaken. Sixty species belonging to five families: Fomitopsidaceae, Ganodermataceae, Meruliaceae, Meripilaceae and Polyporaceae were recorded. Polyporaceae was the dominant family with 46 species identified. The common species encountered based on the number of basidiocarps collected were Ganoderma australe followed by Lentinus …

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Kinetics Of Protein Expression Pattern In Kesum Leaves Or Persicaria Minus / Sharida Binti Shoib, Sharida Shoib Jan 2013

Kinetics Of Protein Expression Pattern In Kesum Leaves Or Persicaria Minus / Sharida Binti Shoib, Sharida Shoib

Student Works (2010-2019)

Proteomics has been applied in various fields such as in medicine and pharmaceutical. Although proteomics has been introduced for almost a decade, the application of proteomics to protein expression in plants can still be considered as new. This present study is aimed at proteins that are involved in leaves senescence. Proteins were extracted from the leaves of the kesum plants at different age. The extracted protein samples were then subjected to separation on one-dimensional electrophoresis (1-DE) and 2-DE. Separation was performed on broad range pH (pH 3-10) gels of 7 cm and narrow range pH (pH 4-7) gels of 7 …

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The Application Of Irap Markers In The Breeding Of Papaya / Bathusha Shareef Bin Kader Shaik Ali, Bathusha Shareef Kader Shaik Ali Jan 2013

The Application Of Irap Markers In The Breeding Of Papaya / Bathusha Shareef Bin Kader Shaik Ali, Bathusha Shareef Kader Shaik Ali

Student Works (2010-2019)

In Malaysia, a papaya breeding program was initiated by the Malaysian Agricultural Research and Development Institute (MARDI) to produce resistant papayas. Nevertheless, selection of resistant papayas from field trials is laborious and time-consuming. In order to facilitate selection of resistant papayas in breeding programs, potential of DNA fingerprinting method was analysed in this study for future applications. Materials from leaves of Carica papaya L. from varieties Tainung 5, Eksotika 6 and their hybrids were used in the assessment of molecular markers. From an analysis of Inter-Retrotransposons Amplified Polymorphism Reaction (IRAP) molecular markers, five combinations out of 45 possible primers combinations …

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Copper Removal By Cellulose Xanthogenate Derived From Imperata Cylindrica L. Leaf Powder / Zubir Bin Othman, Zubir Othman Jan 2013

Copper Removal By Cellulose Xanthogenate Derived From Imperata Cylindrica L. Leaf Powder / Zubir Bin Othman, Zubir Othman

Student Works (2010-2019)

The efficiency of cellulose xanthogenate derived from Imperata cylindrica L. leaf powder (CXIC) for removing Cu(II) from aqueous solutions has been investigated. The effects of physicochemical parameters on biosorption capacities such as biosorbent dosage, pH, initial concentration of Cu(II) and contact time were studied. The biosorption capacities of CXIC increased with the rise in pH and Cu(II) concentration but decreased with the increase in biosorbent dose. Biosorption of Cu(II) was considered fast as the time to reach equilibrium was 60 min. Two kinetic models; pseudo-first order and pseudo-second order were applied to analyse the Cu(II) biosorption process, and it was …

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Application Of Forensic Entomology In Crime Scene Investigations In Malaysia / Kavitha Rajagopal, Kavitha Rajagopal Jan 2013

Application Of Forensic Entomology In Crime Scene Investigations In Malaysia / Kavitha Rajagopal, Kavitha Rajagopal

Student Works (2010-2019)

Forensic entomology is the application and study of insect biology to criminal matters. One of the most important aspects of forensic entomology is the usage of maggot found in dead human body to determine the post-mortem interval (PMI). Based on the number of maggot sent for PMI determination, the application of forensic entomology in crime scene investigation is still unsatisfactory in Malaysia. Hence the present study was the first to conduct a questionnaire survey to determine the degree of knowledge and awareness of forensic entomology in Malaysia. A total of 402 participants comprising of the crime scene police officers, pathologists …

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Identification Of Chemical Constituents In The Extracts Of Pleurotus Giganteus (Berk.) Karunarathna & K. D. Hyde / Seamus Moroney, Seamus Moroney Jan 2013

Identification Of Chemical Constituents In The Extracts Of Pleurotus Giganteus (Berk.) Karunarathna & K. D. Hyde / Seamus Moroney, Seamus Moroney

Student Works (2010-2019)

Pleurotus giganteus mushroom is used for culinary purposes by the indigenous people (Orang Asli) in Malaysia for food. The present study was undertaken to extract and profile the bioactive metabolites in the fruiting bodies of the cultivated mushroom. Various chemical solvents were used in this investigation because different compounds were extracted by different solvents based on the solvent’s polarity. The solvents employed were methanol, hexane, ethyl acetate, distilled water and butanol. The ethyl acetate and hexane extracts were then subjected to flash column chromatography with stepwise addition of hexane and acetone. The identification of compounds in the sub-fractions from ethyl …

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An Exploratory Study On Utilization Of Organic Farming For Sustainable Agriculture At Selected Farms In Malaysia And India / Prasad A/L Vasudevon, Prasad Vasudevon Jan 2013

An Exploratory Study On Utilization Of Organic Farming For Sustainable Agriculture At Selected Farms In Malaysia And India / Prasad A/L Vasudevon, Prasad Vasudevon

Student Works (2010-2019)

Industrial agriculture has become the prominent agriculture system worldwide. In this system, farmers have become less dependent to environmental processes in shaping food production systems. They begin to adapt monoculture farming and rely on increasing quantities of agrochemicals, automated machineries, hybrid seeds and, more recently, Genetically modified crops. However, these practices have caused environmental consequences such as depletion of biodiversity, soil degradation, pollution and global warming. Furthermore concentration and vertical integration of corporate undermines farmer’s participation in the agrifood system. Farmers merely act as technology adopters and passive producers. In this system, farmers’ knowledge and innovations are impeded. In addition …

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Vertebrate Palaeontology From Selected Pleistocene Cave Sites In Perak And Selangor, Peninsular Malaysia / Yasamin Kh. Ibrahim, Yasamin Kh. Ibrahim Jan 2013

Vertebrate Palaeontology From Selected Pleistocene Cave Sites In Perak And Selangor, Peninsular Malaysia / Yasamin Kh. Ibrahim, Yasamin Kh. Ibrahim

Student Works (2010-2019)

Vertebrate fossils consisting of teeth and bones of large mammals from six limestone caves in Lenggong Valley, Perak and Batu Caves, Selangor, in Peninsular Malaysia are reported. This is the first detailed systematic study of a large number of vertebrate fossils found in this country. Samples of the matrix hosting the fossils indicated a Middle Pleistocene age of 500 ka for the Lenggong Valley site and a Late Pleistocene range of 66 - 33 ka for the Batu Caves site by using both the thermoluminescence (TL) and optically-stimulated luminescence (OSL) methods that are applied for the first time in Peninsular …

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University Of Malaya's Low Carbon Approach : Evaluation Of Clean Development Mechanism (Cdm) Potential / Okere Kelechi Justin, Okere Kelechi Justin Jan 2013

University Of Malaya's Low Carbon Approach : Evaluation Of Clean Development Mechanism (Cdm) Potential / Okere Kelechi Justin, Okere Kelechi Justin

Student Works (2010-2019)

Current human energy use and waste management practices have been associated with massive greenhouse gases emission resulting in climate change. Degradation of the already indebted environment and potential threat to sustainability due to the impacts is on the rise and has led to a unique synergy by world leaders to find antidotes. The United Nations Framework Convention on Climate Change (UNFCCC) through Kyoto Protocol in 1997 practically began championing the remedial effort mainly through one of the flexible mechanisms, Clean Development Mechanism (CDM), by which industrialized nations invest in green developments in developing nations and invariably earn carbon credits for …

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Determination Of Heavy Metals In Effluent / Wan Mohd Tarmizi Bin Wan Mohd Yusuf, Wan Mohd Tarmizi Wan Mohd Yusuf Jan 2013

Determination Of Heavy Metals In Effluent / Wan Mohd Tarmizi Bin Wan Mohd Yusuf, Wan Mohd Tarmizi Wan Mohd Yusuf

Student Works (2010-2019)

The objective of this research project is to determine the concentration of 5 toxic metals from industrial effluent.The five metal elements are cadmium (Cd), lead (Pb), copper (Cu), nickel (Ni), and iron(Fe).Two sources of effluent have been taken for the analysis of heavy metals concentration. It is very important to monitor the concentration of toxic metals effluent whether the concentrations are safe to be released to the environment or need to be treated. A serious contamination to human life and environment can occur if there is no observation on the effluent that will be released to the environment. The sample …

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Application Of Hybrid Evolutionary Algorithm And Thematic Map For Rule Set Generation And Visualization Of Chlorophyta Abundance At Putrajaya Lake / Lau Chia Fong, Chia Fong Lau Jan 2013

Application Of Hybrid Evolutionary Algorithm And Thematic Map For Rule Set Generation And Visualization Of Chlorophyta Abundance At Putrajaya Lake / Lau Chia Fong, Chia Fong Lau

Student Works (2010-2019)

This study describes application of a hybrid combination of hybrid evolutionary algorithm (HEA) and thematic map visualization technique in modeling, predicting and visualization of selected algae division growth, Chlorophyta for tropical Putrajaya Lakes and Wetlands (Malaysia). The system was trained and tested using five years of limnological time-series data sampled from tropical Putrajaya Lake and Wetlands (Malaysia). HEA is run on the training set in order to provide insights on the relationships between input variables and the algae abundance. Performances of the rule sets are assessed using Receiving Operating Characteristic (ROC) with true positive rate. The generated rules are tested …

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Proteomic Analysis On Host Responses To Chikungunya Virus Infection / Christina Thio Li Ping, Christina Li Ping Thio Jan 2013

Proteomic Analysis On Host Responses To Chikungunya Virus Infection / Christina Thio Li Ping, Christina Li Ping Thio

Student Works (2010-2019)

Chikungunya virus (CHIKV) is an arthropod-borne virus that has caused multiple unprecedented outbreaks in both tropical and temperate countries over the past five decades. There is no commercial vaccine or antiviral drug to date, due in part to the lack of knowledge and understanding of the biology and pathogenesis of this virus. Thus, there is an increasing need for researchers to focus their research efforts in this area of virology. The current study employed proteomics to investigate alterations of the whole cell proteome and secretome of WRL-68 cells during early CHIKV infection, with the main aim being to identify the …

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Chemically Modified Carbon Nanotubes (Cnts) For Adsorption Of Mercury / Nuruzatulifah Bt Asari @ Mansor, Nuruzatulifah Asari @ Mansor Jan 2013

Chemically Modified Carbon Nanotubes (Cnts) For Adsorption Of Mercury / Nuruzatulifah Bt Asari @ Mansor, Nuruzatulifah Asari @ Mansor

Student Works (2010-2019)

Functionalized multiwalled carbon nanotubes (MWCNTs) for the removal of mercury ion from aqueous solution were studied. This project had two parts, first part was the functionalization of MWCNTs using different method. Second, the performance study of the removal mercury ion using functionalized MWCNTs. Functionalization of MWCNTs was carried out using two separate methods. The first method was a gas phase treatment in a Universal Temperature Program (UTP) reactor by flowing H2SO4/SO3 vapour onto the MWCNTs. The second method was a liquid phase treatment which includes refluxing the MWCNTs in H2SO4. All samples were heated at different temperatures (100, 200, 300 …

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Ethical Implications Of Preimplantation Genetic Diagnosis (Pgd): A Qualitative Study On Three Selected Groups In Malaysia / Angelina Patrick Olesen, Angelina Patrick Olesen Jan 2013

Ethical Implications Of Preimplantation Genetic Diagnosis (Pgd): A Qualitative Study On Three Selected Groups In Malaysia / Angelina Patrick Olesen, Angelina Patrick Olesen

Student Works (2010-2019)

Pre-implantation genetic diagnosis or PGD refers to procedure performed on embryos prior to implantation in IVF trials. The technology enables the embryo to be screened for genetic disease and is usually applied when parents have risk of genetic diseases. Ethical issues raised from the utilization of PGD include gender biasness and discrimination towards people with disabilities often linked to societal obsession with perfectionism. This study explores participant perception of ethical issues posed by PGD, by focusing on four key themes: sanctity of life, play God, perfectionism and freedom of choice. Three focus groups were selected; medical personnel, religious representatives and …

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Selection, Characterization And Evaluation Of Streptomyces For The Control Of Anthracnose In Chilli / Pedram Shahbazi, Pedram Shahbazi Jan 2013

Selection, Characterization And Evaluation Of Streptomyces For The Control Of Anthracnose In Chilli / Pedram Shahbazi, Pedram Shahbazi

Student Works (2010-2019)

The actinomycetes, mainly Streptomyces spp., have been widely studied as potential biocontrol agents against plant pathogenic fungi. This project was undertaken to isolate and select Streptomyces strains showing potential in vitro and in vivo antifungal activity. Isolation was done from rhizosphere-derived soil of healthy and Colletotrichum-infested chilli plants in Ulu Chechuh in Kuala Selangor and Sungai Burung in the state of Selangor, Malaysia. The selected strains were evaluated for their ability to control chilli anthracnose pathogen, Colletotrichum spp. in vitro, and the best strain among them was chosen for green house experimental trials on chilli in vivo. A total of …

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Production Of Bovine Embryos In Vitro Through In Vitro Fertilisation (Ivf) And Intracytoplasmic Sperm Injection (Icsi) Techniques / Nor Fadillah Binti Awang, Nor Fadillah Awang Jan 2013

Production Of Bovine Embryos In Vitro Through In Vitro Fertilisation (Ivf) And Intracytoplasmic Sperm Injection (Icsi) Techniques / Nor Fadillah Binti Awang, Nor Fadillah Awang

Student Works (2010-2019)

The objective of this study was to develop an optimal in vitro produced embryos (IVP) protocol using two methods of insemination, namely in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) protocols by using different qualities of oocytes in Malaysian cattle. The ovaries were collected from slaughtered cattle from the local abattoir. The oocyte morphology was observed and grouped into three groups: Groups A, B and C, based on cumulus cell compactness. Then the oocytes were subdivided based on the cytoplasm quality, namely homogenous and heterogenous (Experiment 1). The oocytes were matured in humidified 5% CO2 incubator at 38.5°C at …

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Production Of Laccase Enzyme Using Rice Husk As Substrate In Fungal Solid-State Fermentation / Teck Nam, Ang, Teck Nam Ang Jan 2013

Production Of Laccase Enzyme Using Rice Husk As Substrate In Fungal Solid-State Fermentation / Teck Nam, Ang, Teck Nam Ang

Student Works (2010-2019)

To produce laccase enzyme in large volume at affordable prices, a fungal solid-state fermentation (SSF) process that uses lignocellulosic by-product from rice milling industry – rice husk was introduced. This research aims to provide deeper insights into major aspects of fungal solid-state fermentation from the selection and pretreatment of solid substrate, selection of fungi to assessment and optimization of the process parameters. The research assesses all the mentioned aspects of solid-state fermentation for the production of laccase enzyme using the oyster mushroom Pleurotus sajor-caju. No pretreatment reagent has been reported effective for pretreating rice husk, the research thus reports on …

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Wound Healing Potential Of Copper (Ii) Bis [N'- (5-Chloro-1hindol-3-Yl) Methyl Nicotinic Hydrazide] On Experimentally Induced Excision Wounds In Rats / Morvarid Rahnama, Morvarid Rahnama Jan 2013

Wound Healing Potential Of Copper (Ii) Bis [N'- (5-Chloro-1hindol-3-Yl) Methyl Nicotinic Hydrazide] On Experimentally Induced Excision Wounds In Rats / Morvarid Rahnama, Morvarid Rahnama

Student Works (2010-2019)

Considering the importance of wounds in human health proclaims the necessity of discovering and developing new effective drug-to-be agents. Cl-indole-nicotinic is a Schiff base compound with promising structural potentials in accelerating healing of wounds. The purpose of present study is to investigate Cl-indole-nicotinic effects on wound healing and acute toxicity on Sprague Dawley rats as well as its activity as an antimicrobial and an antioxidant agent. The manually induced dermal incision on dorsal neck area of four groups of male SD rats were treated for 10 days with high dose compound (50 mg/kg), low dose compound (25 mg/kg), CMC 5%, …

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Method Validation For The Determination Of Hexavalent Chromium In Seawater And Other Matrices By Colorimetric Method / Yong Pui San, Pui San Yong Jan 2013

Method Validation For The Determination Of Hexavalent Chromium In Seawater And Other Matrices By Colorimetric Method / Yong Pui San, Pui San Yong

Student Works (2010-2019)

The research project is about the method validation for the determination of hexavalent chromium in seawater and other matrices by colorimetric method. This method is validated by referring to the APHA 3500 B- Cr Colorimetric Method. The current colorimetric method of determination of hexavalent chromium in wastewater is carried out by using UV-VIS Spectrocopy. This method is developed to determine the trace level of hexavalent chromium up to a minimum 0.001 mg /L or 1 ppb of hexavalent chromium in seawater, river water and industrial effluent by using the Discrete Analyzer. The calibration range is within 0 to 0.25 mg …

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Effects And Control Of Barnacle Infestation On Mangrove Seedlings In A Mangrove Rehabilitation Site / Tan Yee Siang, Yee Siang Tan Jan 2013

Effects And Control Of Barnacle Infestation On Mangrove Seedlings In A Mangrove Rehabilitation Site / Tan Yee Siang, Yee Siang Tan

Student Works (2010-2019)

A study on the development, effects and control of biofouling on replanted mangrove seedlings (40-50cm height) was carried out, from April 2008 to July 2010, at Kampung Sungai Haji Dorani and Kampung Sungai Limau, Selangor, Malaysia. The dominant biofoulers were barnacles (Amphibalanus amphitrite), which were the main causal organism of mangrove seedling damage or death. The high density (>100 no./ 10 cm section of plant stem) and cover (>30 %) of barnacles appeared to obstruct development of new leaves, shoots and roots, as well as imposing weight loads as high as six times the weight of the seedlings. …

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