Digital Commons Network^™

Cross-Scale Morphology, Ahjond S. Garmestani, Craig R. Allen, C. S. Holling

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The scaling of physical, biological, ecological, and social phenomena is a major focus of efforts to develop simple representations of complex systems. Much of the attention has been on discovering universal scaling laws that emerge from simple physical and geometric processes. However, there are regular patterns of departures both from those scaling laws and from continuous distributions of attributes of systems. Those departures often demonstrate the development of self-organized interactions between living systems and physical processes over narrower ranges of scale.

Measuring The Relative Resilience Of Subarctic Lakes To Global Change: Redundancies Of Functions Within And Across Temporal Scales, David G. Angeler, Craig R. Allen, Richard K. Johnson

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

1. Ecosystems at high altitudes and latitudes are expected to be particularly vulnerable to the effects of global change. We assessed the responses of littoral invertebrate communities to changing abiotic conditions in subarctic Swedish lakes with long-term data (1988–2010) and compared the responses of subarctic lakes with those of more southern, hemiboreal lakes.

2. We used a complex systems approach, based on multivariate time-series modelling, and identified dominant and distinct temporal frequencies in the data; that is, we tracked community change at distinct temporal scales. We determined the distribution of functional feeding groups of invertebrates within and across temporal scales. …

Preparing Tomorrow’S Global Leaders: Honors International Education, Mary Kay Mulvaney, Kim Klein

National Collegiate Honors Council Monographs

contents

Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Mary Kay Mulvaney and Kim Klein

Part I: Transformative Pedagogies

Chapter 1: Academic Tourist or Scholar Abroad:

Deep Approaches to Learning. . . . . . . . . . . . . . . . . . . . . . . . . . . . …

Nebraska Forest Service Annual Report 2013, Susan Helmink

Nebraska Forest Service: Publications

Reducing Risks..................................2

The New Normal......................4

Damaging Outbreak..........................................5

Profitable Forests.............................................6

Community Participation..................................................8

Nebraska Champs.............................10

Preserving a Niobrara Gem..........................................................................11

Forestry Education........12

By the Numbers...................................14

Aging Like Fine Wine............................................15

Green Infrastructure................................16

Contributors: Jeanne Andelt Justin Evertson Dr. Mark Harrell Graham Herbst Christina Hoyt Dr. Scott J. Josiah Jessica Kelling Casey McCoy Troy Pabst Jeralyn Schluckebier Jay Seaton Adam Smith Laurie Stepanek Don Westover

Ncpa Newsletter October 2013

Nebraska College Preparatory Academy: Newsletters and Annual Reports

UPCOMING EVENTS

STUDENT SPOTLIGHT

TENTATIVE 2014 SUMMER DATES

2013 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …

Nefdc Exchange, Volume 26, Fall 2013, New England Faculty Development Consortium

New England Faculty Development Consortium Exchange

Contents

President's message - Deborah J. Clark,

Peer-Instruction in your Classroom: A Balancing Act - Dorothy A. Osterholt and Sophie Lampard Dennis, Landmark College

Call for Proposals for the Spring 2014 Conference

Spring conference: June 6, 2014, “Moving from STEM to STEAM: What Really Works”

Build Tomorrow’s Problem Solvers Today: Develop Positive Deviants! - Genevieve E. Chandler, University of Massachusetts-Amherst

Engaged Learning and the Art of Mindfulness in Higher Education - Yvonne Vissing, Salem State University and Michelle Solloway, Greater Los Angeles VA Health Care System

What Can Evolutionary Psychology Teach Us about Pedagogy? - Randy Laist, Goodwin College

Board …

Creating Room For A Singularity Of Our Own: Reading Sue Lange's "We, Robots", Marleen S. Barr

Publications and Research

The accessibility of Lange’s text might mitigate against recognizing its importance. Lange’s simple sentence structure and direct communicative mode convey a presently overlooked logical moral assertion: the impending Singularity is not a male-dominated patriarchal domain. The Singularity, in other words, should not be construed in a manner which excludes women and feminism. This assertion is patently obvious. But, nonetheless, it is often ignored. Before I read Lange’s novella as a description of the Singularity which feminists can embrace, I include the following background information: 1) a discussion about why the discourse relating to the Singularity needs to be expanded and …

Multilocus Sequence Typing For Characterization Of Staphylococcus Pseudintermedius, S. M. Solyman, C. C. Black, B. Duim, V. Perreten, E. Van Duijkeren, J. A. Wagenaar, L. C. Eberlein, L. N. Sadeghi, R. Videla, D. A. Bemis, S. A. Kania

United States Army: Publications

Staphylococcus pseudintermedius is an opportunistic pathogen in dogs. Four housekeeping genes with allelic polymorphisms were identified and used to develop an expanded multilocus sequence typing (MLST) scheme. The new seven-locus technique shows S. pseudintermedius to have greater genetic diversity than previous methods and discriminates more isolates based upon host origin.

Unique Structural Modifications Are Present In The Lipopolysaccharide From Colistin-Resistant Strains Of Acinetobacter Baumannii, Mark R. Pelletier, Leila G. Casella, Jace W. Jones, Mark D. Adams, Daniel V. Zurawski, Karsten R. O. Hazlett, Yohei Doi, Robert K. Ernst

United States Army: Publications

Acinetobacter baumannii is a nosocomial opportunistic pathogen that can cause severe infections, including hospital-acquired pneumonia, wound infections, and sepsis. Multidrug-resistant (MDR) strains are prevalent, further complicating patient treatment. Due to the increase in MDR strains, the cationic antimicrobial peptide colistin has been used to treat A. baumannii infections. Colistin-resistant strains of A. baumannii with alterations to the lipid A component of lipopolysaccharide (LPS) have been reported; specifically, the lipid A structure was shown to be hepta-acylated with a phosphoethanolamine (pEtN) modification present on one of the terminal phosphate residues. Using a tandem mass spectrometry platform, we provide definitive evidence that …

Lrrc6 Mutation Causes Primary Ciliary Dyskinesia With Dynein Arm Defects, Amjad Horani, Thomas W. Ferkol, David Shoseyov, Mollie G. Wasserman, Yifat S. Oren, Batsheva Kerem, Israel Amirav, Malena Cohen-Cymberknoh, Susan K. Dutcher, Steven L. Brody, Orly Elpeleg, Eitan Kerem

Open Access Publications

No abstract provided.

Serological Diagnosis Of North American Paragonimiasis By Western Blot Using Paragonimus Kellicotti Adult Worm Antigen, Peter U. Fischer, Kurt C. Curtis, Scott M. Folk, Patricia P. Wilkins, Luis A. Marcos, Gary J. Weil

Open Access Publications

No abstract provided.

Exposure To Chronic Mild Stress Prevents Kappa Opioid-Mediated Reinstatement Of Cocaine And Nicotine Place Preference, Ream Al-Hasani, Jordan G. Mccall, Michael R. Bruchas

Open Access Publications

No abstract provided.

Persistent Enteric Murine Norovirus Infection Is Associated With Functionally Suboptimal Virus-Specific Cd8 T Cell Responses, Vesselin T. Tomov, Lisa C. Osborne, Douglas V. Dolfi, Gregory F. Sonnenberg, Laurel A. Monticelli, Kathleen Mansfield, Herbert W. Virgin, David Artis, E. John Wherry

Open Access Publications

No abstract provided.

Comparison Of Pcr And Quantitative Real-Time Pcr Methods For The Characterization Of Ruminant And Cattle Fecal Pollution Sources, Meredith R. Raith, Catherine A. Kelty, John F. Griffith, Alexander Schriewer, Stefan Wuertz, Sophie Mieszkin, Michele Gourmelon, Georg H. Reischer, Andreas H. Farnleitner, Jared S. Ervin, Patricia A. Holden, Darcy L. Ebentier, Jennifer A. Jay, Dan Wang, Alexandria B. Boehm, Tiong Gim Aw, Joan B. Rose, E. Balleste, W.G. Meijer, Mano Sivaganesan, Orin C. Shanks

United States Environmental Protection Agency: Publications

The State of California has mandated the preparation of a guidance document on the application of fecal source identification methods for recreational water quality management. California contains the fifth highest population of cattle in the United States, making the inclusion of cow-associated methods a logical choice. Because the performance of these methods has been shown to change based on geography and/or local animal feeding practices, laboratory comparisons are needed to determine which assays are best suited for implementation. We describe the performance characterization of two end-point PCR assays (CF128 and CF193) and five real-time quantitative PCR (qPCR) assays (Rum2Bac, BacR, …

Fumigation Of A Laboratory-Scale Hvac System With Hydrogen Peroxide For Decontamination Following A Biological Contamination Incident, K. M. Meyer, M. W. Calfee, J. P. Wood, L. Michelsen, B. Attwood, M. Clayton, A. Touati, R. Delafield

United States Environmental Protection Agency: Publications

Aims

To evaluate hydrogen peroxide vapor (H₂O₂) for its ability to inactivate Bacillus spores within a laboratory-scale heating, ventilation and air-conditioning (HVAC) duct system.

Methods and Results

Experiments were conducted in a closed-loop duct system, constructed of either internally lined or unlined galvanized metal. Bacterial spores were aerosol-deposited onto 18-mm-diameter test material coupons and strategically placed at several locations within the duct environment. Various concentrations of H₂O₂ and exposure times were evaluated to determine the sporicidal efficacy and minimum exposure needed for decontamination. For the unlined duct, high variability was observed in the …

Effect Of Dietary Treatment With Dimethylarsinous Acid (DmaIii) On The Urinary Bladder Epithelium Of Arsenic (+3 Oxidation State) Methyltransferase (As3mt) Knockout And C57bl/6 Wild Type Female Mice, Puttappa R. Dodmane, Lora L. Arnold, Karen L. Pennington, David J. Thomas, Samuel M. Cohen

United States Environmental Protection Agency: Publications

Chronic exposure to inorganic arsenic (iAs) is carcinogenic to the human urinary bladder. It produces urothelial cytotoxicity and proliferation in rats and mice. DMA^V, a major methylated urinary metabolite of iAs, is a rat bladder carcinogen, but without effects on the mouse urothelium. DMA^III was shown to be the likely urinary metabolite of DMA^V inducing urothelial changes and is also postulated to be one of the active metabolites of iAs. To evaluate potential DMA^III-induced urothelial effects, it was administered to As3mt knockout mice which cannot methylate arsenicals. Female C57BL/6 wild type and As3mt knockout …

Does Location Really Matter? An Inter-Colony Comparison Of Seabirds Breeding At Varying Distances From Productive Oceanographic Features In The Bering Sea, Ann Harding, Rosana Paredes, Robert Suryan, Daniel Roby, David Irons, Rachael Orben, Heather Renner, Rebecca Young, Christopher Barger, Ine Dorresteijn, Alexander Kitaysky

United States Fish and Wildlife Service: Publications

Central place foragers, such as breeding seabirds, need to commute between their nests and foraging grounds, thus close proximity of the breeding colony to productive oceanographic features might be beneficial for seabird reproduction. We tested this hypothesis by investigating the at-sea foraging and breeding behavior of thick-billed murres (Uria lomvia) nesting at three colonies (Bogoslof, St. Paul, and St. George Islands) in the Bering Sea located at different distances from the productive continental shelf-break. We found that distances to feeding are as differed only during night trips among colonies. St. Paul murres foraged entirely on the shelf, whereas …

Impacts Of Migratory Sandhill Cranes (Grus Canadensis) On Microbial Water Quality In The Central Platte River, Nebraska, Usa, Jason R. Vogel, Dale W. Griffin, Hon S. Ip, Nicholas J. Ashbolt, Matthew T. Moser, Jingrang Lu, Mary K. Beitz, Hodon Ryu, Jorge W. Santo Domingo

United States Geological Survey: Staff Publications

Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of birdderived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water …

Exposure Amplitude And Repetition Affect Bottlenose Dolphin Behavioral Responses To Simulated Mid-Frequency Sonar Signals, Dorian S. Houser, Steve W. Martin, James J. Finneran

United States Navy: Publications

Thirty bottlenose dolphins (Tursiops truncatus) participated in a controlled exposure study using an acoustic stimulus that consisted of a simulated tactical sonar signal (1-s duration, 3250–3450 Hz). Each dolphin was trained a behavior requiring it to swim across an enclosure, touch a paddle, and return to the starting location. Ten-trial control and exposure sessions were performed with each dolphin. Dolphins were placed into one of five groups, each of which received a 115, 130, 145, 160, 175 or 185 dB re 1 μPa (rms) sound pressure level (SPL) exposure while crossing the enclosure on exposure trials. A canonical …

Antimicrobial Activity Of Nanoemulsion In Combination With Cetylpyridinium Chloride In Multidrug-Resistant Acinetobacter Baumannii, Yoon Y. Hwang, Karthikeyan Ramalingam, Diane R. Bienek, Valerie Lee, Tao You, Rene Alvarez

United States Navy: Publications

Acinetobacter baumannii has emerged as a serious problematic pathogen due to the ever-increasing presence of antibiotic resistance, demonstrating a need for novel, broad-spectrum antimicrobial therapeutic options. Antimicrobial nanoemulsions are emulsified mixtures of detergent, oil, and water (droplet size, 100 to 800 nm) which have broad antimicrobial activity against bacteria, enveloped viruses, and fungi. Here, we screened the antimicrobial activities of five nanoemulsion preparations against four Acinetobacter baumannii isolates to identify the most suitable preparation for further evaluation. Among them, N5, which contains 10% (vol/vol) Triton X-100, 25% (vol/vol) soybean oil, and 1% (wt/vol) cetylpyridinium chloride (CPC), showed the best efficacy …

The Polysaccharide Capsule Of Campylobacter Jejuni Modulates The Host Immune Response, Alexander C. Maue, Krystle L. Mohawk, David K. Giles, Frédéric Poly, Cheryl P. Ewing, Yuening Jiao, Ginyoung Lee, Zuchao Ma, Mario A. Monteiro, Christina L. Hill, Jason S. Ferderber, Chad K. Porter, M. Stephen Trent, Patricia Guerry

United States Navy: Publications

Campylobacter jejuni is a major cause of bacterial diarrheal disease worldwide. The organism is characterized by a diversity of polysaccharide structures, including a polysaccharide capsule. Most C. jejuni capsules are known to be decorated nonstoichiometrically with methyl phosphoramidate (MeOPN). The capsule of C. jejuni 81-176 has been shown to be required for serum resistance, but here we show that an encapsulated mutant lacking the MeOPN modification, an mpnC mutant, was equally as sensitive to serum killing as the nonencapsulated mutant. A nonencapsulated mutant, a kpsM mutant, exhibited significantly reduced colonization compared to that of wild-type 81-176 in a mouse intestinal …

Identification Of BlaOxa-51-Like, BlaOxa-58, BlaDim-1, And BlaVim Carbapenemase Genes In Hospital Enterobacteriaceae Isolates From Sierra Leone, Tomasz A. Leski, Umaru Bangura, David H. Jimmy, Rashid Ansumana, Stephen E. Lizewski, Robert W. Li, David A. Stenger, Chris R. Taitt, Gary J. Vora

United States Navy: Publications

We describe the results of a molecular epidemiological survey of 15 carbapenemase-encoding genes from a recent collection of clinical isolates from Mercy Hospital in Bo, Sierra Leone. The most salient findings revealed that (i) 60% of the isolates harbored multiple carbapenemase genes; (ii) the bla_DIM-1 gene, which has previously only been reported in The Netherlands, is also circulating in this environment; and (iii) bla_OXA-51-like and bla_OXA-58 genes, which were thought to reside exclusively in Acinetobacter species, can also be found in members of the Enterobacteriaceae.

Application Of Chromosomal Dna And Protein Targeting For The Identification Of Yersinia Pestis, Marilynn A. Larson, Shi-Jian Ding, Shawn R. Slater, Anna Hanway, Amanda M. Bartling, Paul D. Fey, Oksana Lockridge, Stephen C. Francesconi, Steven H. Hinrichs

United States Navy: Publications

Purpose: A comprehensive strategy was developed and validated for the identification of pathogens from closely related near neighbors using both chromosomal and protein biomarkers, with emphasis on distinguishing Yersinia pestis from the ancestral bacterium Yersinia pseudotuberculosis.

Experimental design: Computational analysis was used to discover chromosomal targets unique to Y. pestis. Locus identifier YPO1670 was selected for further validation and PCR was used to confirm that this biomarker was exclusively present in Y. pestis strains, while absent in other Yersinia species. RT-PCR and Western blot analyses were utilized to evaluate YPO1670 expression and MRM MS was performed to identify …

Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with …

Bovine Herpes Virus 1 (Bhv-1) And Herpes Simplex Virus Type 1 (Hsv-1) Promote Survival Of Latently Infected Sensory Neurons, In Part By Inhibiting Apoptosis, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

α-Herpesvirinae subfamily members, including herpes simplex virus type 1 (HSV-1) and bovine herpes virus 1 (BHV-1), initiate infection in mucosal surfaces. BHV-1 and HSV-1 enter sensory neurons by cell-cell spread where a burst of viral gene expression occurs. When compared to non-neuronal cells, viral gene expression is quickly extinguished in sensory neurons resulting in neuronal survival and latency. The HSV-1 latency associated transcript (LAT), which is abundantly expressed in latently infected neurons, inhibits apoptosis, viral transcription, and productive infection, and directly or indirectly enhances reactivation from latency in small animal models. Three anti-apoptosis genes can be substituted for LAT, which …

Original Article Sirt1-Deficient Mice Exhibit An Altered Cartilage Phenotype, Odile Gabay, Kristien J. Zaal, Christelle Sanchez, Mona Dvir-Ginzberg, Viktoria Gagarina, Yingje Song, Xiao Hong He, Michael W. Mcburney

Uniformed Services University of the Health Sciences

tObjective: We previously demonstrated that Sirt1 regulates apoptosis in cartilage in vitro. Here weattempt to examine in vivo cartilage homeostasis, using Sirt1 total body knockout (KO) mice.Method: Articular cartilage was harvested from hind paws of 1-week and 3-week-old mice carrying wildtype (WT) or null Sirt1 gene. Knees of Sirt1 haploinsufficient mice also were examined, at 6 months. Jointcartilage was processed for histologic examination or biochemical analyses of chondrocyte cultures.Results: We found that articular cartilage tissue sections from Sirt1 KO mice up to 3 weeks of age exhibitedlow levels of type 2 collagen, aggrecan, and glycosaminoglycan content. In contrast, protein levels …

Isolation And Genomic Characterization Of Chaoyang Virus Strain Rok144 From Aedes Vexansnipponii From The Republic Of Korea, John S. Lee, Nathan D. Grubaugh, John P. Kondig, Michael J. Turell, Heung-Chul Kim, Terry A. Klein, Monica L. O’Guinn

United States Army: Publications

During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2 km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets^TM, sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into126 pools, one Aedes vexan snipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details …

Construction And Nonclinical Testing Of A Puumala Virus Synthetic M Gene-Based Dna Vaccine, R. L. Brocato, M. J. Josleyn, V. Wahl-Jensen, C. S. Schmaljohn, J. W. Hooper

United States Army: Publications

Puumala virus (PUUV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-lengthMsegment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRG/PUU-M(s2). pWRG/PUU-M(s2) delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG/ PUU-M(s2) protected hamsters against infection with PUUV but not against infection by related HFRS-associated …

Genome Sequence Of Moraxella Macacae 0408225, A Novel Bacterial Species Isolated From A Cynomolgus Macaque With Epistaxis, Jason T. Ladner, Chris A. Whitehouse, Galina I. Koroleva, Gustavo F. Palacios

United States Army: Publications

Moraxella macacae is a recently described bacterial species that has been associated with at least two outbreaks of epistaxis in macaques. Here we present the first genome sequence of this novel species, isolated from a symptomatic cynomolgus macaque at the U.S. Army Medical Research Institute of Infectious Diseases.