Digital Commons Network^™

Effect Of Dietary Treatment With Dimethylarsinous Acid (DmaIii) On The Urinary Bladder Epithelium Of Arsenic (+3 Oxidation State) Methyltransferase (As3mt) Knockout And C57bl/6 Wild Type Female Mice, Puttappa R. Dodmane, Lora L. Arnold, Karen L. Pennington, David J. Thomas, Samuel M. Cohen

United States Environmental Protection Agency: Publications

Chronic exposure to inorganic arsenic (iAs) is carcinogenic to the human urinary bladder. It produces urothelial cytotoxicity and proliferation in rats and mice. DMA^V, a major methylated urinary metabolite of iAs, is a rat bladder carcinogen, but without effects on the mouse urothelium. DMA^III was shown to be the likely urinary metabolite of DMA^V inducing urothelial changes and is also postulated to be one of the active metabolites of iAs. To evaluate potential DMA^III-induced urothelial effects, it was administered to As3mt knockout mice which cannot methylate arsenicals. Female C57BL/6 wild type and As3mt knockout …

Does Location Really Matter? An Inter-Colony Comparison Of Seabirds Breeding At Varying Distances From Productive Oceanographic Features In The Bering Sea, Ann Harding, Rosana Paredes, Robert Suryan, Daniel Roby, David Irons, Rachael Orben, Heather Renner, Rebecca Young, Christopher Barger, Ine Dorresteijn, Alexander Kitaysky

United States Fish and Wildlife Service: Publications

Central place foragers, such as breeding seabirds, need to commute between their nests and foraging grounds, thus close proximity of the breeding colony to productive oceanographic features might be beneficial for seabird reproduction. We tested this hypothesis by investigating the at-sea foraging and breeding behavior of thick-billed murres (Uria lomvia) nesting at three colonies (Bogoslof, St. Paul, and St. George Islands) in the Bering Sea located at different distances from the productive continental shelf-break. We found that distances to feeding are as differed only during night trips among colonies. St. Paul murres foraged entirely on the shelf, whereas …

Impacts Of Migratory Sandhill Cranes (Grus Canadensis) On Microbial Water Quality In The Central Platte River, Nebraska, Usa, Jason R. Vogel, Dale W. Griffin, Hon S. Ip, Nicholas J. Ashbolt, Matthew T. Moser, Jingrang Lu, Mary K. Beitz, Hodon Ryu, Jorge W. Santo Domingo

United States Geological Survey: Staff Publications

Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of birdderived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water …

Exposure Amplitude And Repetition Affect Bottlenose Dolphin Behavioral Responses To Simulated Mid-Frequency Sonar Signals, Dorian S. Houser, Steve W. Martin, James J. Finneran

United States Navy: Publications

Thirty bottlenose dolphins (Tursiops truncatus) participated in a controlled exposure study using an acoustic stimulus that consisted of a simulated tactical sonar signal (1-s duration, 3250–3450 Hz). Each dolphin was trained a behavior requiring it to swim across an enclosure, touch a paddle, and return to the starting location. Ten-trial control and exposure sessions were performed with each dolphin. Dolphins were placed into one of five groups, each of which received a 115, 130, 145, 160, 175 or 185 dB re 1 μPa (rms) sound pressure level (SPL) exposure while crossing the enclosure on exposure trials. A canonical …

Antimicrobial Activity Of Nanoemulsion In Combination With Cetylpyridinium Chloride In Multidrug-Resistant Acinetobacter Baumannii, Yoon Y. Hwang, Karthikeyan Ramalingam, Diane R. Bienek, Valerie Lee, Tao You, Rene Alvarez

United States Navy: Publications

Acinetobacter baumannii has emerged as a serious problematic pathogen due to the ever-increasing presence of antibiotic resistance, demonstrating a need for novel, broad-spectrum antimicrobial therapeutic options. Antimicrobial nanoemulsions are emulsified mixtures of detergent, oil, and water (droplet size, 100 to 800 nm) which have broad antimicrobial activity against bacteria, enveloped viruses, and fungi. Here, we screened the antimicrobial activities of five nanoemulsion preparations against four Acinetobacter baumannii isolates to identify the most suitable preparation for further evaluation. Among them, N5, which contains 10% (vol/vol) Triton X-100, 25% (vol/vol) soybean oil, and 1% (wt/vol) cetylpyridinium chloride (CPC), showed the best efficacy …

The Polysaccharide Capsule Of Campylobacter Jejuni Modulates The Host Immune Response, Alexander C. Maue, Krystle L. Mohawk, David K. Giles, Frédéric Poly, Cheryl P. Ewing, Yuening Jiao, Ginyoung Lee, Zuchao Ma, Mario A. Monteiro, Christina L. Hill, Jason S. Ferderber, Chad K. Porter, M. Stephen Trent, Patricia Guerry

United States Navy: Publications

Campylobacter jejuni is a major cause of bacterial diarrheal disease worldwide. The organism is characterized by a diversity of polysaccharide structures, including a polysaccharide capsule. Most C. jejuni capsules are known to be decorated nonstoichiometrically with methyl phosphoramidate (MeOPN). The capsule of C. jejuni 81-176 has been shown to be required for serum resistance, but here we show that an encapsulated mutant lacking the MeOPN modification, an mpnC mutant, was equally as sensitive to serum killing as the nonencapsulated mutant. A nonencapsulated mutant, a kpsM mutant, exhibited significantly reduced colonization compared to that of wild-type 81-176 in a mouse intestinal …

Identification Of BlaOxa-51-Like, BlaOxa-58, BlaDim-1, And BlaVim Carbapenemase Genes In Hospital Enterobacteriaceae Isolates From Sierra Leone, Tomasz A. Leski, Umaru Bangura, David H. Jimmy, Rashid Ansumana, Stephen E. Lizewski, Robert W. Li, David A. Stenger, Chris R. Taitt, Gary J. Vora

United States Navy: Publications

We describe the results of a molecular epidemiological survey of 15 carbapenemase-encoding genes from a recent collection of clinical isolates from Mercy Hospital in Bo, Sierra Leone. The most salient findings revealed that (i) 60% of the isolates harbored multiple carbapenemase genes; (ii) the bla_DIM-1 gene, which has previously only been reported in The Netherlands, is also circulating in this environment; and (iii) bla_OXA-51-like and bla_OXA-58 genes, which were thought to reside exclusively in Acinetobacter species, can also be found in members of the Enterobacteriaceae.

Application Of Chromosomal Dna And Protein Targeting For The Identification Of Yersinia Pestis, Marilynn A. Larson, Shi-Jian Ding, Shawn R. Slater, Anna Hanway, Amanda M. Bartling, Paul D. Fey, Oksana Lockridge, Stephen C. Francesconi, Steven H. Hinrichs

United States Navy: Publications

Purpose: A comprehensive strategy was developed and validated for the identification of pathogens from closely related near neighbors using both chromosomal and protein biomarkers, with emphasis on distinguishing Yersinia pestis from the ancestral bacterium Yersinia pseudotuberculosis.

Experimental design: Computational analysis was used to discover chromosomal targets unique to Y. pestis. Locus identifier YPO1670 was selected for further validation and PCR was used to confirm that this biomarker was exclusively present in Y. pestis strains, while absent in other Yersinia species. RT-PCR and Western blot analyses were utilized to evaluate YPO1670 expression and MRM MS was performed to identify …

Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with …

Bovine Herpes Virus 1 (Bhv-1) And Herpes Simplex Virus Type 1 (Hsv-1) Promote Survival Of Latently Infected Sensory Neurons, In Part By Inhibiting Apoptosis, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

α-Herpesvirinae subfamily members, including herpes simplex virus type 1 (HSV-1) and bovine herpes virus 1 (BHV-1), initiate infection in mucosal surfaces. BHV-1 and HSV-1 enter sensory neurons by cell-cell spread where a burst of viral gene expression occurs. When compared to non-neuronal cells, viral gene expression is quickly extinguished in sensory neurons resulting in neuronal survival and latency. The HSV-1 latency associated transcript (LAT), which is abundantly expressed in latently infected neurons, inhibits apoptosis, viral transcription, and productive infection, and directly or indirectly enhances reactivation from latency in small animal models. Three anti-apoptosis genes can be substituted for LAT, which …

Original Article Sirt1-Deficient Mice Exhibit An Altered Cartilage Phenotype, Odile Gabay, Kristien J. Zaal, Christelle Sanchez, Mona Dvir-Ginzberg, Viktoria Gagarina, Yingje Song, Xiao Hong He, Michael W. Mcburney

Uniformed Services University of the Health Sciences

tObjective: We previously demonstrated that Sirt1 regulates apoptosis in cartilage in vitro. Here weattempt to examine in vivo cartilage homeostasis, using Sirt1 total body knockout (KO) mice.Method: Articular cartilage was harvested from hind paws of 1-week and 3-week-old mice carrying wildtype (WT) or null Sirt1 gene. Knees of Sirt1 haploinsufficient mice also were examined, at 6 months. Jointcartilage was processed for histologic examination or biochemical analyses of chondrocyte cultures.Results: We found that articular cartilage tissue sections from Sirt1 KO mice up to 3 weeks of age exhibitedlow levels of type 2 collagen, aggrecan, and glycosaminoglycan content. In contrast, protein levels …

Isolation And Genomic Characterization Of Chaoyang Virus Strain Rok144 From Aedes Vexansnipponii From The Republic Of Korea, John S. Lee, Nathan D. Grubaugh, John P. Kondig, Michael J. Turell, Heung-Chul Kim, Terry A. Klein, Monica L. O’Guinn

United States Army: Publications

During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2 km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets^TM, sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into126 pools, one Aedes vexan snipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details …

Construction And Nonclinical Testing Of A Puumala Virus Synthetic M Gene-Based Dna Vaccine, R. L. Brocato, M. J. Josleyn, V. Wahl-Jensen, C. S. Schmaljohn, J. W. Hooper

United States Army: Publications

Puumala virus (PUUV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-lengthMsegment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRG/PUU-M(s2). pWRG/PUU-M(s2) delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG/ PUU-M(s2) protected hamsters against infection with PUUV but not against infection by related HFRS-associated …

Genome Sequence Of Moraxella Macacae 0408225, A Novel Bacterial Species Isolated From A Cynomolgus Macaque With Epistaxis, Jason T. Ladner, Chris A. Whitehouse, Galina I. Koroleva, Gustavo F. Palacios

United States Army: Publications

Moraxella macacae is a recently described bacterial species that has been associated with at least two outbreaks of epistaxis in macaques. Here we present the first genome sequence of this novel species, isolated from a symptomatic cynomolgus macaque at the U.S. Army Medical Research Institute of Infectious Diseases.

University Of Nebraska- Lincoln: Fact Book 2012-2013

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

Fact Book Table of Contents

Introduction ............................................................................ 1

Table of Contents .................................................................... 2

General Information

University of Nebraska-Lincoln Core Values (LEADERS)............................... 5

The Role of the University of Nebraska-Lincoln ................................................. 6

The Missions of the University of Nebraska-Lincoln ......................................... 6

Teaching..................................................................................................... 7

Research....................................................................................................... 8

Service.............................................................................................. 8

Institutional and Professional Accreditations................................................ 9

University of Nebraska-Lincoln Administrative Organization Chart ................... 12

Student Credit Hours (SCH)

Total SCH: Fall Semester Since 1979 ............................................................... 13

Total SCH: Spring Semester Since 1993 .................................................... 14

SCH by College and Course Level, Fall and Spring Semester - 5 Year Trend .... 15

SCH by College and Course …

Water Current, Volume 45, No. 1, Winter 2013

Water Current Newsletter

Winter Storm Cancels January WRAP Meeting

Two Interview for Nebraska Water Center Directorship

UNL Scientist Cites Managing Risks in New Keystone XL Pipeline Route

Meet the Faculty - Derek M. Heeren

From the Interim Director: Interviewing Permanent Director

Expect More Impacts as Nebraska Drought Rolls into Second Year

The Nebraska Water Resources Association

Free Public Water Lectures Continue Through April

2013 Water and Natural Resources Tour Is June 25-27: Managing Nebraska's Water Resources

Climate Masters of Nebraska Creating Informed Activists

Drought Webinar Series

February Planning Trip for the June Water and Natural Resources Tour

News from the EPA: EPA Releases …

Odorous Voc Emission Following Land Application Of Swine Manure Slurry, David B. Parker, John Gilley, Bryan Woodbury, Ki-Hyun Kim, Geordie Galvin, Shannon L. Bartelt-Hunt, Xu Li, Daniel D. Snow

Nebraska Water Center: Faculty Publications

Swine manure is often applied to crop land as a fertilizer source. Odor emissions from land-applied swine manure may pose a nuisance to downwind populations if manure is not applied with sufficient forethought. A research project was conducted to assess the time decay of odorous volatile organic compound (VOC) emissions following land application of swine manure. Three land application methods were compared: surface application, incorporation 24 h after surface application, and injection. Emission rates were measured in field plots using a small wind tunnel and sorbent tubes. VOCs including eight volatile fatty acids, five aromatics, and two sulfur-containing compounds were …

Heifer Calving Date Positively Influences Calf Weaning Weights Through Six Parturitions, R. A. Cushman, L. H. Kill, Richard N. Funston, E. M. Mousel, G. A. Perry

West Central Research and Extension Center, North Platte

Longevity and lifetime productivity are important factors influencing profitability for the cowcalf producer. Heifers that conceive earlier in the breeding season will calve earlier in the calving season and have a longer interval to rebreeding. Calves born earlier in the calving season will also be older and heavier at weaning. Longevity data were collected on 2,195 heifers from producers in South Dakota Integrated Resource Management groups. Longevity and weaning weight data were collected on 16,549 individual heifers at the U.S. Meat Animal Research Center (USMARC). Data were limited to heifers that conceived during their first breeding season. Heifers were grouped …

Spatio-Temporal Dynamics Of Pneumonia In Bighorn Sheep, E. Frances Cassirer, Raina K. Plowright, Kezia R. Manlove, Paul C. Cross, Andrew P. Dobson, Kathleen A. Potter, Peter J. Hudson

United States Geological Survey: Staff Publications

1. Bighorn sheep mortality related to pneumonia is a primary factor limiting population recovery across western North America, but management has been constrained by an incomplete understanding of the disease. We analysed patterns of pneumonia-caused mortality over 14 years in 16 interconnected bighorn sheep populations to gain insights into underlying disease processes.

2. We observed four age-structured classes of annual pneumonia mortality patterns: all-age, lamb-only, secondary all-age and adult-only. Although there was considerable variability within classes, overall they differed in persistence within and impact on populations. Years with pneumonia-induced mortality occurring simultaneously across age classes (i.e. all-age) appeared to be …

Protein Kinases: Structure Modeling, Inhibition, And Protein-Protein Interactions, Khaled M. Elokely

Electronic Theses and Dissertations

Human protein kinases belong to a large and diverse enzyme family that contains more than 500 members. Deregulation of protein kinases is associated with many disorders, and this is why protein kinases are attractive targets for drug discovery. Due to the high conservation of the ATP binding pocket among this family, designing specific and/or selective inhibitors against certain member(s) is challenging. Several studies have been conducted on protein kinases to validate them as suitable drug targets. Although there are numerous target-validated protein kinases, the efforts to develop small molecule inhibitors have so far led to only a limited number of …

The Design, Synthesis, And Biological Evaluation Of Aplysinopsin Analogs As Potential Neuromodulators, Kevin Lewellyn

Electronic Theses and Dissertations

Aplysinopsins are tryptophan-derived natural products that have been isolated from a variety of marine organisms and have been shown to possess a range of biological activities. Initial synthesis of a library of 50 aplysinopsin analogs revealed that of the 12 serotonin receptor subtypes and 34 other CNS receptors, aplysinopsin analogs shoa high affinity for the 5-HT2B and 5-HT2C receptor subtypes, with selectivity for 5-HT2B over 5-HT2C. Bromination at C-4 and C-5 of the indole ring resulted in greater binding affinities, with Ki's as low as 35 nM. In addition, biological evaluation of the MAO-A and MAO-B inhibitory activities of these …

Vol. 42, No. 1: Full Issue, Denver Journal Of International Law & Policy

Denver Journal of International Law & Policy

No abstract provided.

Dynamics Of Shame In Genesis 3 And 4: Missiological Implications For Today, Andrew Tompkins

Journal of Adventist Mission Studies

No abstract provided.

Cultural Actions Toward The Dead In Selected Cultures In Africa: Missiological Implications, Gaius A. Umahi

Journal of Adventist Mission Studies

No abstract provided.

A Brief History Of The Development Of Minnesota's Criminal Law, Bradford Colbert, Frances Kern

William Mitchell Law Review

No abstract provided.

2013 Presentation Abstracts, Grand Valley State University

Academic Conference Fund Abstracts

No abstract provided.

The Role Of Disgust In Eating Disorders, Eun Ha Kim

Electronic Theses and Dissertations

Disgust, a basic emotional response, which influences approach/avoidance behaviors, has been studied in various psychological disorders, including anxiety, depression, and eating disorders. The current study employed exploratory correlations and hierarchical linear regressions (controlling for race, anxiety, and depression) in order to be able to observe the relationships among three traditional domains of disgust (i.e., core, animal-reminder, contamination) and eating disordered behaviors. Results revealed significant correlations between core disgust and dieting behaviors, in addition to contamination disgust and bulimia and symptoms of food preoccupation as well as behaviors concerning self-control around food-related stimuli (i.e., oral control). More importantly, disgust sensitivity was …

Mounds In The Landscape: A Comparative Study Of Landscape Archaeology In English-Speaking Northwest Europe And North America, Jennifer Ann Rich

Electronic Theses and Dissertations

Humans have been intrigued by their surrounding landscape for centuries. Sometimes intrigue has led to particular manipulations of the land by groups of people, such as the building of mounds and other monuments. Thus, the study of past landscape use is an important part in understanding our own interests. Over the years, developments in archaeology have come to include various perspectives on how past landscapes should be interpreted. This thesis will examine the changes within the theoretical perspectives in landscape archaeology through the decades. Within the regions of English-speaking Northwest Europe (including Britain and Ireland) and North America, I will …

Reconsidering The Theoretical/Practical Divide: The Philosophy Of Nishida Kitarō, Lockland Vance Tyler

Electronic Theses and Dissertations

Over the years professional philosophy has undergone a number of significant changes. One of these changes corresponds to an increased emphasis on objectivity among philosophers. In light of new discoveries in logic and science, contemporary analytic philosophy seeks to establish the most objective methods and answers possible to advance philosophical progress in an unambiguous way. By doing so, we are able to more precisely analyze concepts, but the increased emphasis on precision has also been accompanied by some negative consequences. These consequences, unfortunately, are much larger and problematic than many may even realize. What we have eventually arrived in at …

The Darker Angels Of Our Nature: The South In American Horror Film, Steven Clayton Saunders

Electronic Theses and Dissertations

How does one make a region horrific? For well over half of a century, the American South has functioned as a site for national anxieties over race and modernization. This study uses an inter-disciplinary approach in order to understand the various forces involved in the construction of the South in American horror cinema. Particular attention is paid to the influence that images of the civil rights movement have had on the development and evolution of the South as a horrific and terrifying space for the rest of the nation. It focuses on four main subcategories of the genre: the white …