Digital Commons Network^™

Acanthamoeba Genotype T4 Detected In Naturally-Infected Feline Corneas Found To Be In Homology With Those Causing Human Keratitis, I. Ithoi, R. Mahmud, M.H.A. Basher, A. Jali, A.M. Abdulsalam, J. Ibrahim, J.W. Mak

Research Publications (2011 to 2015)

A total of 10 out of 65 cornea swab samples from cats with eye symptoms showed Acanthamoeba-like morphology after cultivation. By PCR and DNA sequencing of Acanthamoeba diagnostic fragment 3 (DF3), all 10 isolates from the positive samples were categorized into two homologous groups of AfC1 (PM1, PM2, PM3, PF6, KM7, KF8, KMK9) and AfC2 (PM4, PM5, KFK10) due to the presence of bases A(354) and G(354), respectively. Furthermore, DF3 of AfC1 and AfC2 showed 100 similarity with Genbank reference isolates with the accession numbers DQ087314, EU146073 and U07401, GU808323, which were Acanthamoeba castellanii strains genotype T4 originating from human …

Alpha-Tomatine Attenuation Of In Vivo Growth Of Subcutaneous And Orthotopic Xenograft Tumors Of Human Prostate Carcinoma Pc-3 Cells Is Accompanied By Inactivation Of Nuclear Factor-Kappa B Signaling, S.T. Lee, P.F. Wong, H. He, J.D. Hooper, Mohd Rais Mustafa

Research Publications (2011 to 2015)

Background: Nuclear factor-kappa B (NF-κB) plays a role in prostate cancer and agents that suppress its activation may inhibit development or progression of this malignancy. Alpha (α)-tomatine is the major saponin present in tomato (Lycopersicon esculentum) and we have previously reported that it suppresses tumor necrosis factor-alpha (TNF-α)-induced nuclear translocation of nuclear factor-kappa B (NF-κB) in androgen-independent prostate cancer PC-3 cells and also potently induces apoptosis of these cells. However, the precise mechanism by which α-tomatine suppresses NF-κB nuclear translocation is yet to be elucidated and the anti-tumor activity of this agent in vivo has not been examined. Methodology/ Principal …

Amoebic Forms Of Blastocystis Spp. -Evidence For A Pathogenic Role, A. Rajamanikam, Suresh Kumar Govind

Research Publications (2011 to 2015)

Background: Blastocystis spp. are one of the most prevalent parasites isolated from patients suffering from diarrhea, flatulence, constipation and vomiting. Itâ��s pathogenicity and pathophysiology remains controversial to date. Protease activity and amoebic forms have been reported previously in symptomatic isolates but there has been no conclusive evidence provided to correlate the protease activity and any specific life cycle stage of the parasite thus far. Methods: Symptomatic isolates with amoebic form were tested for protease activity and compared with symptomatic and asymptomatic isolates without amoebic form for 10 days culture period. Results: The present study demonstrates an elevated protease activity in …

Anti-Inflammatory, Gastroprotective And Anti-Ulcerogenic Effects Of Red Algae Gracilaria Changii (Gracilariales, Rhodophyta) Extract, M.H. Shu, D. Appleton, Keivan Zandi, Sazaly Abubakar

Research Publications (2011 to 2015)

Background: Gracilaria changii (Xia et Abbott) Abbott, Zhang et Xia, a red algae commonly found in the coastal areas of Malaysia is traditionally used for foods and for the treatment of various ailments including inflammation and gastric ailments. The aim of the study was to investigate anti-inflammatory, gastroprotective and anti-ulcerogenic activities of a mass spectrometry standardized methanolic extract of Gracilaria changii. Methods: Methanolic extract of Gracilaria changii (MeOHGCM6 extract) was prepared and standardized using mass spectrometry (MS). Anti-inflammatory activities of MeOHGCM6 extract were examined by treating U937 cells during its differentiation with 10 mu g/ml MeOHGCM6 extract. Tumour necrosis factors-alpha …

Aporphine Alkaloids From The Leaves Of Phoebe Grandis (Nees) Mer. (Lauraceae) And Their Cytotoxic And Antibacterial Activities, H. Omar, N.M. Hashim, A. Zajmi, N. Nordin, S.I. Abdelwahab, A.H.S. Azizan, A.H.A. Hadi, Hapipah Mohd Ali

Research Publications (2011 to 2015)

The oxoaporphine alkaloid lysicamine (1), and three proaporphine alkaloids, litsericinone (2), 8,9,11,12-tetrahydromecambrine (3) and hexahydromecambrine A (4) were isolated from the leaves of Phoebe grandis (Nees) Merr. (Lauraceae). Compounds 2 and 3 were first time isolated as new naturally occurring compounds from plants. The NMR data for the compounds 2–4 have never been reported so far. Compounds 1 and 2 showed significant cytotoxic activity against a MCF7 (human estrogen receptor (ER+) positive breast cancer) cell line with IC50 values of 26 and 60 µg/mL, respectively. Furthermore, in vitro cytotoxic activity against HepG2 (human liver cancer) cell line was evaluated for …

Armigeres Subalbatus Incriminated As A Vector Of Zoonotic Brugia Pahangi Filariasis In Suburban Kuala Lumpur, Peninsular Malaysia, A. Muslim, Mun Yik Fong, Rohela Mahmud, Yee Ling Lau, S. Sivanandam

Research Publications (2011 to 2015)

Background: In 2011, we reported occurrence of natural human infections with Brugia pahangi, a filarial worm of dogs and cats, in a surburb of Kuala Lumpur, the capital city of Malaysia. Our preliminary entomological survey at that time suggested the mosquito species Armigeres subalbatus as the vector of the zoonotic infections. In this present report, we provide biological evidence to confirm our preliminary finding. Findings: A total of 1798 adult female Ar. subalbatus mosquitoes was caught in the vicinity of the suburb, and 1599 were dissected for the presence of filarial larvae. Sixty-two mosquitoes were positive, and 27 of these …

Building A Dna Barcode Reference Library For The True Butterflies (Lepidoptera) Of Peninsula Malaysia: What About The Subspecies?, J.J. Wilson, K.W. Sing, Mohd Sofian-Azirun

Research Publications (2011 to 2015)

The objective of this study was to build a DNA barcode reference library for the true butterflies of Peninsula Malaysia and assess the value of attaching subspecies names to DNA barcode records. A new DNA barcode library was constructed with butterflies from the Museum of Zoology, University of Malaya collection. The library was analysed in conjunction with publicly available DNA barcodes from other Asia-Pacific localities to test the ability of the DNA barcodes to discriminate species and subspecies. Analyses confirmed the capacity of the new DNA barcode reference library to distinguish the vast majority of species (92) and revealed that …

Scanner: An Efficient And Accurate Trimming Tool For Illumina Next Generation Sequencing Reads, Xiang Zhou

All Computer Science and Engineering Research

Recent advances in High-Throughput Sequencing (HTS) technology have greatly facilitated the researches in bioinformatics field. With the ultra-high sequencing speed and improved base-calling accuracy, Illumina Genome Analyzer is currently the most widely used platform in the field. To use the raw reads generated from the sequencing machine, the 3’ adapter sequence attached to the real read in the process of ligation needs to be correctly trimmed. This is often done by some inhouse scripts or different packages with various parameters. They either use the Smith-Waterman algorithm or search for an exact match of the 3’ adapter sequence. In this report, …

The Individual Mandate's Due Process Legality: A Kantian Explanation, And Why It Matters, Peter Brandon Bayer

Scholarly Works

In National Federation of Independent Business v. Sebelius, one of the most controversial decisions of this young century, an intensely divided Supreme Court upheld the Patient Protection and Affordable Care Act's most provocative feature-the Individual Mandate-under Congress's taxing power. In so doing, the Court rejected what appeared to be the Individual Mandate's more applicable constitutional premise-Congress's authority to regulate interstate commerce. Yet, neither the Constitution's Taxing Clause nor its Commerce Clause provide the ultimate answer as to whether Congress may regulate the multi-billion dollar healthcare market by compelling unwilling persons to buy private health insurance. The final determination of the …

From “Food Miles” To “Moneyball”: How We Should Be Thinking About Food And Climate, Bret C. Birdsong

Scholarly Works

Since Michael Pollan popularized the push to eat local food in his bestseller, The Omnivore’s Dilemma, the concept of “food miles” has been something of a rallying cry and an organizing principle in the marketing of the local food movement. Among locavores and their sympathizers, the term seems to encapsulate all that is wrong with the food system. Fresh grapes from Chile make their way to supermarkets from Maine to Minnesota, and even California. Major food conglomerates process commodity ingredients like corn, soy, and wheat into packaged food that travels across the country and across oceans before landing on …

Developmental Detour: How The Minimalism Of Miller V. Alabama Led The Court's "Kids Are Different" Eighth Amendment Jurisprudence Down A Blind Alley, Mary E. Berkheiser

Scholarly Works

No abstract provided.

Where Do The Prophets Stand?: Hamdi, Myth And The Master's Tools, Linda H. Edwards

Scholarly Works

No abstract provided.

Consumer Perceptions Of Poultry Production In Arkansas: Perceptions Analysis, Stuart Estes, Leslie D. Edgar

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Poultry production holds an important place in Arkansas economically and as a food source. The importance of poultry production ultimately hinges on the demands of the consumer. With this in mind, this study surveyed consumers to assess their perceptions of poultry production in Arkansas. The instrument, used to survey consumers, was created by the researcher and an expert committee at the University of Arkansas. Consumers were interviewed through direct communication at grocery stores in northwest Arkansas. Data gathered from the study were analyzed for descriptive and correlational statistics. Two key findings were that consumers were unsure about the use of …

Bat Activity Over Wetlands: Temporal And Spatial Variation, Sarah Stankavich

EWU Masters Thesis Collection

"Foraging animals face decisions about when and where to forage based, in part, on prey availability, which can fluctuate temporally as well as spatially. For temperate insectivorous bats, wetlands are important forage habitats because they provide water and high abundances of insects. My overall objective was to identify abiotic and biotic factors that influence bat foraging activity over wetlands in the channeled scablands of eastern Washington. I surveyed 12 wetland sites at Turnbull National Wildlife Refuge (TNWR) in Cheney, Washington, 3 or 4 times during summer 2012. Ten stations were set up at each site to measure insect abundance using …

The Ogbanje In Little Bee By Chris Cleave, Courtney A. Harler

EWU Masters Thesis Collection

"In Chris Cleave's 2008 novel Little Bee, he offers readers a literary representation of the Nigerian spirit-child for the purpose of political and post-colonial examination. Cleave's intentions are revealed through a full study of this traditional Nigerian concept. The Nigerian spirit-child, known as the ogbanje in the lgbo language and the abiku in the Yoruba language of Western Nigeria, is imprisoned in an endless cycle of birth, death, and rebirth. The concept of the ogbanje-abiku is indeed related to the overall concept of reincarnation, but it differs in the sense that the child who is unduly linked to the spirit …

Dna Barcoding Of Jatropha Curcas, Terence L. Stephens

EWU Masters Thesis Collection

"DNA barcoding involves sequencing a standard region of DNA to be used as a tool for rapid species identification. The cytochrome C oxidase gene (COXI) is widely accepted as the standard barcode for animals and currently there is no barcode for land plants. The Consortium for the Barcode of Life (CBOL) has proposed two regions of DNA to be used as a dual-locus barcode. In this study I examine the two proposed barcoding regions, ribulose-1 ,5-bisphosphate carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and …

Efficacy Of Tamoxifen And Thymoquinone On Tumor Development, Lipid Peroxidation, And Total Antioxidant Capacity In Dmba Induced Mammary Carcinoma In Female Sprague-Dawley Rats, Nadiah S. Alotaibi

EWU Masters Thesis Collection

"Tamoxifen (TAM) is a drug used for treatment and prevention of breast cancer. However, TAM has been reported to induce liver tumors in several animal models due to overproduction of free radicals and lipid peroxidation. Thymoquinone (TQ), from Nigella sativa seeds, is a Middle East natural medicine and it has been used to treat a number of diseases, including cancer. The aim of my study was to investigate the effect of TQ and TAM on tumor development, lipid peroxidation level, and total antioxidant capacity in DMBA treated female Sprague-Dawley rats. Five groups of 10 rats (control, DMBA, TAM, TQ, and …

The Lamona Rockshelter: Cordage From The Columbia Plateau, Melodie Peterson-Bailey

EWU Masters Thesis Collection

No abstract provided.

"The Worry That You Are Yourself": Darl's Unforgivable Neurodiversity In As I Lay Dying, Neal Hallgarth

EWU Masters Thesis Collection

"This paper is concerned with the character of Darl Bundren and the repeated motif of madness in William Faulkner's As I Lay Dying. Possible psychiatric explanations for Darl's madness, including linguistic evidence for his psychotic breakdown, are explored. The paper does not agree with the critical use of words such as "insane" or "crazy" as the book itself questions the verity of such labels. Critical analyses concerning Darl tend to be sympathetic but use derogatory language when defending the character. Articles related to the relatively new idea of neurodiversity will counter the backhanded sympathy with which the critics regard Darl. …

Assessing Naturalistic Decision Making By Experienced And Inexperienced Interrogators In High Stakes Interviews, Amy Bethany Ross

Open Access Theses & Dissertations

The current thesis aimed to improve the ecological validity of human intelligence interrogation research. Although field practitioners and researchers have worked together to improve the scientific validity and practical execution of interrogation techniques, research has yet to understand how, when and why interrogators implement such techniques. This thesis investigated the active decision making processes of interrogators that occur during high value interrogations. The theory of Naturalistic Decision Making was used to capture the naturalistic characteristics of high value interrogations- ambiguity, time pressure and high stakes. To capture the decision-making processes that are associated with skilled performance, this research conducted in-depth …

Refining Molecular Approaches For Bacterial Source Tracking (Bst) In Texas, Joy Truesdale

Open Access Theses & Dissertations

Bacterial source tracking (BST) is a new science that aids in identifying sources of human and animal fecal contamination of environmental water. There are currently two approaches used in BST, library-dependent and library-independent methods. The fecal bacterium E. coli is often used in library dependent methods because it indicates fecal contamination, has been used in human health risk assessments, and has water quality regulatory significance. A disadvantage of E. coli library dependent BST methods is that identification libraries of E. coli isolated from known fecal sources are needed to identify the sources of E. coli isolated from water samples. Further, …

Shadows On The Wall, Daniel Bruce Greenhalgh

Open Access Theses & Dissertations

Thom Roth has spent his entire life in the Refuge, a secure and fundamentally hierarchical facility which protects the remnants of human civilization. Under the tutelage of Minister Trask, Thom has risen to become a primary contender for an administrative appointment, as well as secured the affections of Dee McKay, Trask's granddaughter. However, when Thom's best friends, Geoff and Scott, discover a small, secret tunnel underneath the Refuge, the three friends' learn that the foundation of their home was decidedly messier than they had been taught. On the other side of the tunnel they find another, smaller Refuge, with fewer …

Ruthenium-Azole Complexes As Chemotherapeutic Agents Against Leishmaniasis, Eva Iniguez

Open Access Theses & Dissertations

Leishmaniasis is a neglected disease that affects millions of people worldwide. Poor efficacy and high toxicity of the limited treatment options demonstrate the need for the development of new chemotherapeutic agents to treat this disease. In order to respond this concern, the development of novel Ru-azole complexes as chemotherapeutic agents by inhibiting the cytochrome P-450 dependent C14-α-demethylation of lanosterol to ergosterol which is essential for the parasite survival. In addition, Ruthenium would enhance the activity of the parental drug, by helping ketoconazole/clotrimazole to cross the parasite membrane, together presenting low toxicity. The viability and citotoxicity of the Ru-azole compunds was …

Changes In Net Ecosystem Production Over The Past 40 Years In Arctic Tundra Ponds Near Barrow, Alaska: Application Of Historic And Modern Techniques, Nickole Ann Miller

Open Access Theses & Dissertations

Arctic environments have gained interest recently due to their sensitivity to climate change. As warming has created longer growing seasons, extended ice-free periods as well as permafrost thaw, the production in freshwater ecosystems has the potential to be greatly impacted. It is hypothesized that as climate change and warming occurs, increases in primary production will occur and ponds will become carbon sinks. Net ecosystem production (NEP) was determined using phytoplankton and periphyton samples collected weekly during the growing season (June - August) from arctic tundra ponds near Barrow, Alaska. Enrichment with 14C was used to determine both weekly photosynthetic rates …

How I Rode The Bus To Become A Professor At The University Of Denver Sturm College Of Law; Reflections On Keyes's Legacy For The Metropolitan, Post-Racial, And Multiracial Twenty-First Century, Tom I. Romero Ii

Scholarly Works

No abstract provided.

We Are Always Already Imprisoned: Hyper-Incarceration And Black Male Identity Performance, Frank Rudy Cooper

Scholarly Works

In this Essay, Professor Frank Rudy Cooper recenters the experiences of men of color, particularly those of black men, in light of Reagan's War on Drugs and recent scholarship illustrating the over-representation of men of color in prison for petty drug use. The mainstream's depiction of black men as always already imprisoned disciplines us into the never-finished quest to prove we are a "Good Black Man," rather than a "Bad Black Man." In order to propose greater empathy for black men's imprisonment, this article proceeds in the following manner. In Part I, Professor Cooper sets the stage for considering the …

What Cognitive Dissonance Tells Us About Tone In Persuasion, Kathryn M. Stanchi

Scholarly Works

This Article takes the first step in thinking about where good advocacy should draw the line between zeal and coercion. Legal advocates differ about how to navigate that line.' Is the best service to the client to be found in the most aggressive, strongest, hard-line approach? Or is a more tempered, reasonable approach most likely to produce the best results?

This Article looks at cognitive science for guidance on this question. One cognitive process that seems to be integral to tone is cognitive dissonance, a concept I will explain in Part II. I then take a close look at two …

Ships Of The Fleet: An Essay On The Weltanschauung Of The Apostolate And The Mission Of The Spirit, Art Mcphee

The Asbury Journal

In honor of Bishop Sundo Kim

This paper was presented in Asbury Theological Seminary, on November 14, 2012, at Dr. Art McPhee’s installation in the Sundo Kim Chair for Evangelism and Practical Theology Expertise: Evangelization Studies. In this essay, he explores the role of the Holy Spirit in mission and evangelism as an often-overlooked subject in the Church today.

Engaging Witchcraft Accusations Among Christians As A Vehicle Of African Traditional Religious Self-Advocacy In African Contexts, Robert Magoola

The Asbury Journal

That African Christianity is growing in numbers has become a familiar fact; testimony to the commitment of African preachers and adherents to the gospel of Christ. However, a disturbing trend is sweeping over many parts of sub-Saharan Africa in places such as Uganda. It is the increased number of witchcraft accusations that are being leveled against Christians and Christian leaders. In this paper, Robert Magoola acknowledges this unfortunate predicament facing the African church as it maligns Christian gospel proclamation and enhances African traditional religion’s self- advocacy. He evaluates the possible credibility of field-based evidence on the basis of a parable …

Understanding Christian Perfection And Its Struggle With Antinomianism, Victoria L. Campbell

The Asbury Journal

Every so often, a professor at Asbury Theological Seminary will notice a current student with exceptional promise. The Asbury Journal wants to help highlight the work of rising academics by publishing works from such students. This paper is an example of such a work, brought to the attention of the editor by Dr. Larry Wood.

Much of the confusion regarding John Wesley’s phrase, Christian perfection, comes from the western tendency to define “perfection” as a state of infallibility (from the Latin perfectio) rather than a process of spiritual maturing based upon the Greek word for perfection, teleios (Matthew …