Digital Commons Network^™

Designed Guanidinium-Rich Amphipathic Oligocarbonate Molecular Transporters Complex, Deliver And Release Sirna In Cells, E. I. Geihe, Christina B. Cooley, J. R. Simon, M. K. Kiesewetter, J. A. Edward, R. P. Hickerson, R. L. Kaspar, J. L. Hedrick, R. M. Waymouth, P. A. Wender

Chemistry Faculty Research

The polyanionic nature of oligonucleotides and their enzymatic degradation present challenges for the use of siRNA in research and therapy; among the most notable of these is clinically relevant delivery into cells. To address this problem, we designed and synthesized the first members of a new class of guanidinium-rich amphipathic oligocarbonates that noncovalently complex, deliver, and release siRNA in cells, resulting in robust knockdown of target protein synthesis in vitro as determined using a dual-reporter system. The organocatalytic oligomerization used to synthesize these co-oligomers is step-economical and broadly tunable, affording an exceptionally quick strategy to explore chemical space for optimal …

Annual Review Of The World Pheasant Association, 2011/2012, World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Reports and Other Materials

Chairman of Trustees' report, Richard Carden

Obituary: Reinhard Kruger (1938?-June 14, 2012)

Treasurer's report for the year ended April 30, 2012, Jon Riley

Achieving our charitable objectives, Philip J. K. McGowan

Galliformes Specialist Group, Peter J. Garson and Ilse Storch

Conserving Djibouti's priority land animals, Philip J.K. McGowan

Assessing the future of species on the brink, Matt Grainger

Myanmar workshop, Philip J. K. McGowan

What is the status of peafowl in Vietnam? Tommaso Savini, Vy Nguyen, and Niti Sukumal

Highlights from the European Conservation Breeding Group, Ludo Pinceel

Pipar project

Assessment Of The Role Of Solute Carrier Drug Transporters In The Systemic Disposition Of Fluoroquinolones: An In Vitro - In Vivo Comparison, Aditi Mulgaonkar

Theses and Dissertations

Fluoroquinolones (FQ) are broad-spectrum charged antimicrobials exhibiting excellent tissue/fluid permeation. Thus, FQ disposition depends essentially on active transport and facilitative diffusion. Although most early transporter studies investigating renal elimination of FQs have focused on apical efflux of FQs from renal proximal tubule cell (RPTC) into urine, their basolateral uptake mechanism(s) from blood into RPTC (i.e., first step to tubular secretion) has not yet been explored in detail. Renally expressed SLC22 members: organic anion (OATs) and cation (OCTs) transporters are known to transport such small organic ionic substrates (molecular weight ~400 Da). Hence it is of interest to explore the role …

August 2012 Commencement Program, Bowling Green State University

Commencement Programs

No abstract provided.

“That Pregnancy Can Bring Noise Into The Family”: Exploring Intimate Partner Sexual Violence During Pregnancy In The Context Of Hiv In Zimbabwe, Simukai Shamu, Naeemah Abrahams, Marleen Temmerman, Tamara Shefer, Christina Zarowsky

Obstetrics and Gynaecology, East Africa

Background: Globally, studies report a high prevalence of intimate partner sexual violence (IPSV) and an association with HIV infection. Despite the criminalisation of IPSV and deliberate sexual HIV infection in Zimbabwe, IPSV remains common. This study explored women’s and health workers’ perspectives and experiences of sexuality and sexual violence in pregnancy, including in relation to HIV testing.

Methods: This qualitative study was part of a larger study of the dynamics of intimate partner violence and HIV in pregnancy in Zimbabwe. Key informant interviews were conducted with health workers and focus group discussions were held with 64 pregnant or nursing mothers …

The Geographical Dimension Of The Development Effects Of Natural Resources, Abdur Chowdhury, Fabrizio Carmignani

Economics Faculty Research and Publications

We study the contribution of natural resource intensity to long-term development along different dimensions: per-capita income, institutional quality, and education. We allow natural resources to affect these dimensions differently in different regions of the world. The evidence suggests that natural resources are generally a positive driver of development, but in Sub-Saharan Africa (SSA) their contribution is almost negligible, if not even negative. We explain these cross-regional differences with the fact that in SSA more than anywhere else large resource endowments are combined with a particularly bad disease environment. Some historical evidence and formal econometric results support this hypothesis.

Experiences With Social Services Among Homeless Lgbtq Young Adults, Fallon Kadel

HIM 1990-2015

My findings imply that homeless LGBTQ young adults are very limited in where they can go to find LGBTQ-accepting services. Even though all the shelters that were interviewed claimed they did not discriminate against LGBTQ persons, they do not provide and are not affiliated with services or programs that are considered to be LGBTQ- accepting. Based on the research, shelters would rather have clients conform to their pre-existing structure rather than the shelters conforming to the specific needs of the clients.; The goal of this research is to explore what programs and services are being provided at various homeless shelters …

Synergistic Effects Of Nanosecond Pulsed Electric Fields Combined With Low Concentration Of Gemcitabine On Human Oral Squamous Cell Carcinoma In Vitro, Jing Wang, Jinsong Guo, Shan Wu, Hongqing Feng, Shujun Sun, Jie Pan, Jue Zhang, Stephen J. Beebe

Bioelectrics Publications

Treatment of cancer often involves uses of multiple therapeutic strategies with different mechanisms of action. In this study we investigated combinations of nanosecond pulsed electric fields (nsPEF) with low concentrations of gemcitabine on human oral cancer cells. Cells (Cal-27) were treated with pulse parameters (20 pulses, 100 ns in duration, intensities of 10, 30 and 60 kV/cm) and then cultured in medium with 0.01 mu g/ml gemcitabine. Proliferation, apoptosis/necrosis, invasion and morphology of those cells were examined using MTT, flow cytometry, clonogenics, transwell migration and TEM assay. Results show that combination treatments of gemcitabine and nsPEFs exhibited significant synergistic activities …

Dissolute Pluralism: An Ethics Of Queer Thought, Kristin P. Mccartney

College of Liberal Arts & Social Sciences Theses and Dissertations

Every practice has a theory, but multiracial queer social movements have centered theory in their praxis. In consciousness-raising and affinity groups, listservs and electronic forums, zines and community action research projects, participants frame the task of thought itself as collective and interactive, subject not just to evolving norms, but also to vicissitudes of duration, location, and timing, medium, ritual, and personality. I argue that the ephemeral character of these interventions is not incidental or regrettable, but designed to encourage organic participation and obstruct institutional codification. In turn, these values and tactics call academics to interrogate how they enter into this …

Yeast Surface Display Of Cd154, Iaryna Masniuk

Graduate Theses and Dissertations

The CD154 (CD40L) is a member of tumor necrosis factor (TNF) family that plays a crucial role in regulation of both cell-mediated and humoral immunity by activating antigen presenting cells (APCs). Previously, studies in the laboratory demonstrated that yeast surface display system allows the expression of biologically functional antigen, such as subunit HA, against influenza virus. Yeast surface display system seems to be an excellent system to be utilized to develop anti-influenza virus vaccine. Thus, it is of interest to study the adjuvant function of CD154 on the subunit HA vaccine against influenza virus. In this project, the CD154 encoding …

Comparing Single-Sex And Reformed Coeducation: A Constitutional Analysis, Nancy Chi Cantalupo

San Diego Law Review

One of the most enduring educational debates of the past three decades has dealt with the legality and advisability of sex-segregated education. This debate can often look confusing, given a large number of debaters and the diversity of their perspectives and agendas. More than this diversity, however, the debate is confusing because the debate has been structured as a contest between the "innovation" of sex-segregated education and status quo coeducation. Missing from the debate is a comparison between reformed coeducation and a single-sex alternative, a comparison that is markedly more useful in determining what ought to be done about the …

Adolescent Identity Versus The First Amendment: Sexuality And Speech Rights In The Public Schools, Steven J. Macias

San Diego Law Review

This Article examines the legal tensions that currently exist between public schools' attempts to protect gay students from bullying and harassment and antigay students' First Amendment rights to engage in allegedly harassing speech. First, it looks at the popular conception of the relationship between childhood or adolescence and sexuality by considering the political and legal fallout of the same-sex marriage debate. Then this Article turns to a conception of public schooling that would allow for the constitutionally permissible regulation of antigay-identity speech. Next, through the use of critical theory, this Article explains why First Amendment defenses to gay harassment have …

Institutional Data Book 2011-2012, University Of Minnesota, Morris Office Of Institutional Research

Institutional Effectiveness and Research Reports

No abstract provided.

Managing Pasture As A Crop - Darrell Emmich, Darrell Emmick

Faculty & Staff Scholarship

No abstract provided.

Long-Term Survival Of Anoxia Despite Rapid Atp Decline In Embryos Of The Annual Killifish Austrofundulus Limnaeus., Jason Podrabsky, Michael Menze, Steven Hand

Faculty and Staff Scholarship

Embryos of the annual killifish Austrofundulus limnaeus can survive for months in the complete absence of oxygen. Survival of anoxia is associated with entry into a state of metabolic dormancy known as diapause. However, extreme tolerance of anoxia is retained for several days of post-diapause development. Rates of heat dissipation in diapause II and 4 days post-diapause II embryos were measured under aerobic conditions and during the transition into anoxia. Phosphorylated adenylate compounds were quantified in embryos during entry into anoxia and after 12 hr of aerobic recovery. Rates of heat dissipation were not affected by exposure to anoxia in …

Depression As A Risk Factor For Breast Cancer : Investigating Methodological Limitations In The Literature., Patrick Pössel, Erica Adams, Jeffrey C. Valentine

Faculty and Staff Scholarship

Purpose: A relationship between depression and the development of breast cancer has not been convincingly shown in the research conducted over the past three decades. Methods: In an effort to better understand the conflicting results, a review was conducted focusing on the methodological problems associated with this literature, including time frame between the assessment of depression and the diagnosis of breast cancer and the use of somatic items in measuring depression. Fifteen breast cancer prospective studies were reviewed. Results: While twelve of the studies found positive associations between depression and breast cancer development, three studies found negative associations. With regards …

Carrs Surveillance Study: Design And Methods To Assess Burdens From Multiple Perspectives, Manisha Nair, Mohammed K. Ali, Vamadevan S. Ajay, Roopa Shivashankar, Viswanathan Mohan, Rajendra Pradeepa, Mohan Deepa, Hassan Mehmood Khan, Muhammad Masood Kadir, Zafar Fatmi, Srinath Srinath Reddy, Nikhil Tandon, Km Venkat Narayan, Dorairaj Prabhakaran

Community Health Sciences

Background: Cardio-metabolic diseases (CMDs) are a growing public health problem, but data on incidence, trends, and costs in developing countries is scarce. Comprehensive and standardised surveillance for non-communicable diseases was recommended at the United Nations High-level meeting in 2011.
Aims: To develop a model surveillance system for CMDs and risk factors that could be adopted for continued assessment of burdens from multiple perspectives in South-Asian countries.
Design: Hybrid model with two cross-sectional serial surveys three years apart to monitor trend, with a three-year prospective follow-up of the first cohort.Sites: Three urban settings (Chennai and New Delhi in India; Karachi in …

Aloe Striata Plant Defense Compounds Produced In Response To Jasmonic Acid, Salicylic Acid, And Eriophyid Mite Salivary Extract Elicitor Compounds, Fritz Light

Master's Theses

Aloe mites are herbivores of the genus Aloe (ALOACEAE) and are associated with hyperplastic growth in various aloe species, but the biochemistry of this interaction is poorly understood. In an effort to characterize plant defense responses to herbivory in the genus Aloe, a salivary extract was isolated from aloe mites (Aceria aloinis Keifer) and its bioactivity was tested using a hypocotyl elongation assay. Subsequently, Aloe striata plants were treated with jasmonic acid (JA), salicylic acid (SA), and the mite salivary extract. Using water and methanol, compounds of different polarity were extracted from aloe tissues that had been frozen …

Advancing Global Tobacco Control: Exploring Worldwide Youth Attitudes And Behaviors Toward Tobacco Use And Control, Sreenivas Phanikumar Veeranki

Electronic Theses and Dissertations

Tobacco use continues to be the leading cause of preventable death in the world. The disproportionate increase in tobacco use in low- and middle-income countries needs immediate attention. Many smokers begin smoking as adolescents and are most likely to become permanent smokers. Moreover, youth are highly targeted by tobacco industry strategies. However, a gap exists in literature to understand worldwide youth tobacco use and control. The purpose of this study is to 1) identify factors that influence never-smoking youths‟ smoking susceptibility, 2) explore characteristics that influence youth exposure to environmental tobacco smoke (ETS) and 3) to delineate key determinants of …

Impact Of Hybrid Rice On Food Security: A Spatial Equilibrium Analysis Of Global Adoption And Diffusion Of Hybrid Rice Varieties, Till Ludwig

Graduate Theses and Dissertations

Rice is the staple crop for most consumers in Asia. More than half of the global population depends on rice. However, scarce resources for agricultural production and unfavorable conditions will make it hard to meet future demand in rice and threaten future food security. Hybrid rice technology is a method to increase the productivity of resources needed for rice production. Current developments show not only yield improvements in comparison to existing conventional and hybrid varieties, but also fuel hopes for new abiotic and biotic stress tolerance. The objective of this study is to determine what impact hybrid rice varieties can …

Contextual Influences On Small Group Decision-Making: A Field Investigation, Sarah Kathryn Chorley

Graduate Theses and Dissertations

This thesis examines small group decision-making among groups of educators in an elementary school through the Bona Fide Group Perspective, which focuses on a group and its relationships to elements in its context. Twenty-six faculty members were interviewed and 26 meetings were recorded; these were analyzed using thematic analysis. The results indicated contextual, relational, and discursive factors influenced the decision-making process. Most critically, power, status, overlapping group memberships, and competing Discourses shaped the group decision-making process.

Assessing Knowledge Acquisition Through Lecture And Cooperative Learning: Traditional And Non-Traditional Secondary Students In Agriculture, Beth Ann Jean Bills-Hunt

Graduate Theses and Dissertations

Currently, students entering secondary agricultural education programs have less knowledge and hands-on experience about agriculture than previous generations. Agricultural educators are challenged to vary teaching practices to meet student needs. The purpose of this study was to determine if there is a difference in knowledge acquisition between students enrolled in secondary agricultural education classes taught by cooperative learning and lecture instruction. Additionally, this study explored the knowledge acquisition of traditional and non-traditional secondary agricultural education students and perceptions of instructional methods presented. Eight schools participated in the study. No significant difference was seen in test scores between methods of instruction, …

Mass Latex Balloon Releases And The Potential Effects On Wildlife, Stephan Irwin

All Dissertations

The first component of the study examined the fate of balloons after release to determine aspects of where, in what state, and how long they persist in the environment to assess potential risk of exposure to wildlife. Tagged balloons from sporting events gave estimates of mean distances traveled from releases. Effects on the structural integrity of the latex balloon as it reaches the upper atmosphere were also tested to determine the physical state when fragments landed. Degradation studies were conducted to determine the length of time latex can persist in the environment after exposure to various environmental conditions (sun, shade, …

The Crayfish (Decapoda: Cambaridae) Subgenus Hiaticambarus: Undescribed Diversity And Life History Characteristics Of Selected Members, Danny Jones

All Dissertations

A taxonomic study of the crayfish species Cambarus (Hiaticambarus) longirostris was conducted. Multivariate and univariate statistical analyses of morphometric data and examination of morphological characters revealed the existence of four undescribed species from populations previously considered to belong to C. longirostris; these were located on the southern extents of the range of C. longirostris. Three of these new species were morphologically similar to the group of species containing C. coosawattae, C. chasmodactylus, C. elkensis, C. longirostris, C. longulus, and C. manningi, while one was morphologically similar to the C. fasciatus, C. …

Lignin Modification In Arabidopsis And Populus For Studies Of Gene Function And Improving Lignin Degradation, Yi Xu

All Dissertations

Lignin is one of the most abundant biopolymers in plants and plays an important role in plant structure and stress defense. Lignin is also considered to be a hallmark of vascular plants because of its crucial role in plant terrestrialization. However, lignin is an undesired component in the pulp and paper industry, bioethanol production, and forage digestibility. Thus, understanding the functions and the evolution of lignin biosynthesis genes can not only advance our knowledge of the evolution of land-adaptation for vascular plants but also help guide the effort to exploit the potential for genetic manipulation of lignin for desirable traits …

Investigating The Role Of Mechanosensitive Ion Channels In Urothelial Cell Pressure Mechanotransduction, Shawn Olsen

All Dissertations

Overactive bladder (OAB) is a bladder disorder that is characterized by bladder storage symptoms of urgency with or without urge incontinence, frequency, nocturia, and, as of 2003, affected approximately 16.5% of adults in the United States with an annual treatment cost of over $65 billion. While therapies are available to mitigate the symptoms of OAB, there are no treatments for the cause of OAB, due to the lack of understanding of the etiology of the disorder. Recent research has provided evidence that the bladder urothelium is not just a passive barrier, but is also sensitive to various chemical and mechanical …

Adolescent Perceptions Of Digital Play: A Study In Third-Person Effects, Wendy Blanchard

All Dissertations

ADOLESCENT PERCEPTIONS OF DIGITAL PLAY:
A STUDY IN THIRD-PERSON EFFECTS
ABSTRACT
With the third-person effect as a conceptual framework, this study examined perceived effects of digital media and electronic devices among charter high school students in the Southeastern United States. In studying third-person perceptions, the study built on research by Schmierbach, Boyle, Xu and McLeod (2011), who analyzed perceived effects of digital gaming among college students.
In addition to items addressing the positive and negative effects of digital media and electronic devices, participants in the current research responded to questions about time spent exercising and in the outdoors, time spent …

The Influence Of Flagship Species On In Situ And Ex Situ Wildlife Tourists' Connection To Wildlife And Pro-Conservation Behaviors, Jeffrey Skibins

All Dissertations

Annually, millions of tourists visit natural areas and zoos primarily to view flagship species such as lions and elephants. Venues rely on the inherent charisma of these species to increase visitation and anchor conservation efforts. Expected visitor outcomes from the use of flagships include raised levels of awareness and pro-conservation behaviors. However, the role of flagships in wildlife tourism has been criticized for not delivering conservation benefits for species of interest or biodiversity, and producing negative site impacts. Furthermore, little is known about how the connection to a species influences conservation behaviors. This dissertation addresses this gap in knowledge by …

Essays In Family Economics, Bing Bai

All Dissertations

The first chapter examines the effect of the number of years children spend living with a single-parent family instead of a two-parent family on children's completed schooling, based on a sample of children from the PSID. To deal with the endogeneity of mothers' family structure decisions, I exploit the variation across states and over time in unilateral divorce laws, unmarried fertility ratios, welfare rules, earned income tax credit rates, and labor market conditions that generate plausibly exogenous changes in mothers' family structure choices. I construct a set of extensive measures for these contextual variables and use them as instruments to …

Ecology And Phylogeny Of The Biting-Midge Genus Culicoides (Diptera: Ceratopogonidae), Dustin Swanson

All Dissertations

Flies of the genus Culicoides are small, biting midges in the family Ceratopogonidae. These flies are of medical, veterinary, and economic importance because their blood-feeding behavior can cause stress to hosts and transmit disease agents. Despite the importance of these flies, little is known about their biology, especially the ecology of the immature stages and the phylogenetic relationships among taxa. The objectives of my study were to address these two areas of Culicoides biology.
Larval Culicoides were collected from aquatic habitats in four ecoregions of South Carolina, USA. Eleven ecological variables were recorded for each sample. Larvae were identified by …