Digital Commons Network^™

A Conserved Two-Component Regulatory System, Pids/Pidr, Globally Regulates Pigmentation And Virulence-Related Phenotypes Of Burkholderia Glumae, Hari Sharan Karki, Inderjit Kaur Barphagha, Jong Hyun Ham

Faculty Publications

Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B.glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B.glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, …

Inferring The Evolutionary History Of Divergence Despite Gene Flow In A Lizard Species, Scincella Lateralis (Scincidae), Composed Of Cryptic Lineages, Nathan D. Jackson, Christopher C. Austin

Faculty Publications

Although recent radiations are fruitful for studying the process of speciation, they are difficult to characterize and require the use of multiple loci and analytical methods that account for processes such as gene flow and genetic drift. Using multilocus sequence data, we combine hierarchical cluster analysis, coalescent species tree inference, and isolation-with-migration analysis to investigate evolutionary relationships among cryptic lineages of North American ground skinks. We also estimate the extent that gene flow has accompanied or followed diversification, and also attempt to account for and minimize the influence of gene flow when reconstructing relationships. The data best support seven largely …

Sperm Cryopreservation In Live-Bearing Xiphophorus Fishes: Offspring Production From Xiphophorus Variatus And Strategies For Establishment Of Sperm Repositories, Huiping Yang, Rafael Cuevas-Uribe, Markita G. Savage, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Cryopreservation of sperm from Xiphophorus fishes has produced live young in three species: X. hellerii, X. couchianus, and X. maculatus. In this study, the goal was to establish protocols for sperm cryopreservation and artificial insemination to produce live young in X. variatus, and to identify needs for repository development. The objectives were to: 1) collect basic biological characteristics of males; 2) cryopreserve sperm from X. variatus, 3) harvest live young from cryopreserved sperm, and 4) discuss the requirements for establishment of sperm repositories. The 35 males used in this study had a body weight of 0.298±0.096g (mean±SD), body length of …

The Faculty Notebook, September 2012, Provost's Office

Faculty Notebook

The Faculty Notebook is published periodically by the Office of the Provost at Gettysburg College to bring to the attention of the campus community accomplishments and activities of academic interest. Faculty are encouraged to submit materials for consideration for publication to the Associate Provost for Faculty Development. Copies of this publication are available at the Office of the Provost.

Assessment Of Co-Occurring Depression And Substance Use In An Ethnically Diverse Patient Sample During Behavioral Health Intake Interviews, Sarah Darghouth, Ora Nakash, Alisa Miller, Margarita Alegría

School for Social Work: Faculty Publications

Background: Co-occurring disorders present a challenge for providers who often fail to diagnose them with accuracy. This study explores the assessment process of co-occurring depressive and substance use disorders in community health clinics serving ethnically diverse patients. The goals are to identify how symptoms discussed in intake, as well as patient and provider characteristics, are associated with receiving a diagnosis of co-occurring disorders or not. Methods: Participation in the study consisted of videotaping the intake, conducting a semi-structured interview, and completing demographic and clinical measures. Quantitative analyses were conducted based on information coded from videotapes of intakes with 119 patients …

Sub-Antarctic And High Antarctic Notothenioid Fishes: Ecology And Adaptational Biology Revealed By The Icefish 2004 Cruise Of Rvib Nathaniel B. Palmer, H. William Detrich Iii, Bradley A. Buckley, Daniel F. Doolittle, Christopher D. Jones, Susanne J. Lockhart

Biology Faculty Publications and Presentations

The goal of the ICEFISH 2004 cruise, which was conducted on board RVIB Nathaniel B. Palmer and traversed the transitional zones linking the South Atlantic to the Southern Ocean, was to compare the evolution, ecology, adaptational biology, community structure, and population dynamics of Antarctic notothenioid fishes relative to the cool/temperate notothenioids of the sub-Antarctic. To place this work in a comprehensive ecological context, cruise participants surveyed the benthos and geology of the biogeographic provinces and island shelves on either side of the Antarctic Polar Front (or Antarctic Convergence). Genome-enabled comparison of the responses of cold-living and temperate notothenioids to heat …

Borrelia Burgdorferi Cp32 Bpab Modulates Expression Of The Prophage Nucp Nuclease And Ssbp Single-Stranded Dna-Binding Protein, Alicia M. Chenail, Brandon L. Jutras, Claire A. Adams, Logan H. Burns, Amy Bowman, Ashutosh Verma, Brian Stevenson

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Borrelia burgdorferi BpaB proteins of the spirochete's ubiquitous cp32 prophages are DNA-binding proteins, required both for maintenance of the bacteriophage episomes and for transcriptional regulation of the cp32 erp operons. Through use of DNase I footprinting, we demonstrate that BpaB binds the erp operator initially at the sequence 5′-TTATA-3′. Electrophoretic mobility shift assays indicated that BpaB also binds with high affinity to sites located in the 5′ noncoding regions of two additional cp32 genes. Characterization of the proteins encoded by those genes indicated that they are a single-stranded DNA-binding protein and a nuclease, which we named SsbP and NucP, …

The Death Of A Disco Dancer; A Sense Of Order And Other Stories; The Scholar Of Moab, Shelah M. Miner, David M. Clark, Jack Harrell, Steven L. Peck

BYU Studies

DAVID CLARK. The Death of a Disco Dancer. Provo, Utah: Zarahemla Books, 2011.

JACK HARRELL. A Sense of Order and Other Stories. Salt Lake City: Signature Books, 2010.

STEVEN L. PECK. The Scholar of Moab. Torrey, Utah: Torrey House Press, 2011.

As an enthusiastic reader of literary fiction and as someone who is fascinated by Mormon culture, I am always on the lookout for works of literary fiction that contain Mormon themes or Mormon characters. While there are always plenty of new romances on the shelves at Deseret Book, and Mormon authors frequently find commercial and critical success writing science …

Racial And Ethnic Disparities In Post-Disaster Mental Health: Examining The Evidence Through A Lens Of Social Justice, Jonathan Purtle

Washington and Lee Journal of Civil Rights and Social Justice

No abstract provided.

Advancing Culturally And Linguistically Appropriate Services At All Phases Of A Disaster, C. Godfrey Jacobs, Darci L. Graves, Jennifer Kenyon, Guadalupe Pacheco

Washington and Lee Journal of Civil Rights and Social Justice

No abstract provided.

Ask, Tell, But Do Not Get Greedy: The Inequalities That Pervade In The Military In Light Of The Repeal Of “Don’T Ask, Don’T Tell”, David Barnes

Washington and Lee Journal of Civil Rights and Social Justice

No abstract provided.

Investigation Of A New Material For Heart Valve Tissue Engineering, Claire Brougham, Gerard Cooney, Thomas Flanagan, Stefan Jockenhoevel, Fergal O'Brien

Conference Papers

No abstract provided.

President's Newsletter, Brooks Keel

President's Newsletter

No abstract provided.

Spr Bulletin, Fall 2011 & Spring 2012, Uno Office Of Research And Creative Activity

Sponsored Programs Bulletins

This bulletin features Recent Grant Recipients and SPR News.

Variation In Genes Related To Cochlear Biology Is Strongly Associated With Adult-Onset Deafness In Border Collies, Jennifer S. Yokoyama, Ernst T. Lam, Alison L. Ruhe, Carolyn A. Erdman, Kathryn R. Robertson, Aubrey A. Webb, D. Colette Williams, Melanie L. Chang, Marjo K. Hytönen, Hannes Lohi, Steven P. Hamilton, Mark W. Neff

Anthropology Faculty Publications and Presentations

The domestic dog offers a unique opportunity to study complex disorders similar to those seen in humans, but within the context of the much simpler genetic backgrounds of pure breeds, which represent closed populations. We performed a whole-genome search for genetic risk factors of adult-onset deafness in the Border Collie, a breed of herding dog that relies on acute hearing to perceive and respond to commands while working. Adult-onset deafness in Border Collies typically begins in early adulthood and is similar to age-related hearing loss in humans. This earlier onset has particular impact on the utility of working Border Collies …

Maximal Force Characteristics Of The Ca2+-Powered Actuator Of Vorticella Convallaria, Sangjin Ryu, Matthew J. Lang, Paul Matsudaira

Department of Mechanical and Materials Engineering: Faculty Publications

The millisecond stalk contraction of the sessile ciliate Vorticella convallaria is powered by energy from Ca²⁺ binding to generate contractile forces of ~10 nN. Its contractile organelle, the spasmoneme, generates higher contractile force under increased stall resistances. By applying viscous drag force to contracting V. convallaria in a microfluidic channel, we observed that the mechanical force and work of the spasmoneme depended on the stalk length, i.e., the maximum tension (150–350 nN) and work linearly depended on the stalk length (~2.5 nN and ~30 fJ per 1 mm of the stalk). This stalk-length dependency suggests that motor units of …

Endogenous Inhibitor Proteins That Connect Ser/Thr Kinases And Phosphatases In Cell Signaling., Masumi Eto, David L Brautigan

Department of Molecular Physiology and Biophysics Faculty Papers

Protein phosphatase activity acts as a primary determinant of the extent and duration of phosphorylation of cellular proteins in response to physiological stimuli. Ser/Thr protein phosphatase-1 (PP1) belongs to the PPP superfamily, and is associated with regulatory subunits that confer substrate specificity, allosteric regulation, and subcellular compartmentalization. In addition, all eukaryotic cells contain multiple heat-stable proteins that originally were thought to inhibit phosphatase catalytic subunits released from the regulatory subunits, as a fail-safe mechanism. However, discovery of C-kinase-activated PP1 inhibitor, Mr of 17 kDa (CPI-17) required fresh thinking about the endogenous inhibitors as specific regulators of particular phosphatase complexes, acting …

Center For Quantitative And Qualitative Research Methods, Todd C. Headrick, Rhonda K. Kowalchuk, Yanyan Sheng, Jennifer Koran

Reports

No abstract provided.

Ensc 360.01: Applied Ecology, Vicki J. Watson

University of Montana Course Syllabi

No abstract provided.

Low Power Data Acquisition For Microimplant Biometric Monitoring Of Tremors, Tania Khanna '01

Doctoral Dissertations

In recent years, trends in the medical industry have created a growing demand for implantable medical devices. In particular, the need to provide doctors a means to continuously monitor biometrics over long time scales with increased precision is paramount to efficient healthcare. To make medical implants more attractive, there is a need to reduce their size and power consumption. Small medical implants would allow for less invasive procedures, greater comfort for patients, and increased patient compliance. Reductions in power consumption translate to longer battery life. The two primary limitations to the size of small medical implants are the batteries that …

Oncolog, Volume 57, Number 09, September 2012, Zach Bohannan, Sunita Patterson

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Stereotactic Body Radiation Therapy Achieves High Control Rates in Lung Cancer: Stereotactic body radiation (SBRT), a relatively new treatment modality, has recently become a very successful treatment-and may even exceeded the effectiveness of surgery-for early-stage lung cancer
• Inflammatory Breast Cancer: Clinical Challenge, Research Enigma- Inflammatory breast cancer (IBC) stands apart from other cancers of the breast in its unusual clinical presentation, its aggressiveness, and its poor prognosis. Researchers and clinicians are working to clarify what distinguishes IBC from other breast cancers and to discover treatments that improve patient outcomes
• HOUSE CALL: Reducing Cancer-Causing Chemicals in Outdoor Cooking: Tips for healthy …

Between Heaven And Earth: Christian Perspectives On Environmental Protection (Book Review), Jeff Ploegstra

Pro Rege

Reviewed Title: Van Dyke, Fred. Between Heaven and Earth: Christian Perspectives on Environmental Protection. Santa Barbara: Praeger, 2010. 237 pages. ISBN 978-0-313-37536-1.

An Anatomical, Histological And Ultrastructural Study On The Placenta Of The Red-Shanked Douc Langur (Pygathrix Nemaeus), Damri Darawiroj, Natthaya Tuaprakone, Yongchai Utara

The Thai Journal of Veterinary Medicine

A red-shanked douc langur (Pygathrix nemaeus nemaeus) is currently enlisted as an endangered primatespecies. This study was undertaken to describe the gross anatomy, histology and ultrastructural morphology of thedouc langur term placenta by light, scanning, and transmission electron microscopy. The primary disc of bidiscoidalplacenta was separated to 4-6 small cotyledonary subdivisions and was attached by an umbilical cord. The chorionfrondosum of placenta was classified as villous, haemomonochorial and deciduate type. The interhaemal area wascomposed of a layer of syncytiotrophoblast that occasionally clustered as the syncytial knot, fetal capillaryendothelium and its basement membrane. The intervillous mineralized fibrinoid mass and syncytial knot …

Five Year Issue-Based Review: September 2012. A Comprehensive Study Of The Southeast District Of University Of Nebraska–Lincoln Extension

Southeast Research and Extension Center: Reports and Administration

CONTENTS

1. Vision and Overview ... 5-8

2. Next Generation Extension ... 9

a New Delivery Systems ... 11-14

b Measuring Return on Investments ... 15-19

c Partnerships ... 20-23

d Translational Research ... 24-29

e Staying on the Cutting Edge ... 30-31

3. Agriculture and Science Literacy ... 33-34

a Livestock ... 35-39

b Crops ... 40-43

c Water ... 44-47

d Nutrition ... 48-51

4. SREC Contributions: ... 53

IANR Growing a Healthy Future – 2025 Goals

a Food

Crops ... 55-59

Nutrition ... 60-64

Livestock ... 65-66

b Water

Crops & Urban ... 67-68

c People

4-H …

Plains Harvest Mouse (Reithrodontomys Montanus Griseus): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding species of interest. The Nebraska Natural Legacy Project recognizes the plains harvest mouse (Reithrodontomys montanus griseus) as a Tier I at-risk species of high conservation need. Some general management recommendations are made here regarding the plains harvest mouse; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the plains harvest mouse …

Topeka Shiner (Notropis Topeka): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the Topeka shiner (Notropis topeka) as a Tier I at-risk species of high priority for conservation. Some general management recommendations are made here regarding Topeka shiners; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the Topeka shiner that …

The Wild Civilized: An Environmental Art Studio Installed Outdoors In An Institutional Space, Neil Goss

Textile Society of America: Symposium Proceedings

In 2011, I set up an outdoor dyeing and weaving studio that also functioned as a sculptural installation, The Earthway Studio. The purpose was multi-dimensional: to educate the university community and the public about culturally pluralistic fiber processes such as spinning, dyeing and weaving, to glean and process a bulk of my materials on site and to inspire others through demonstrations and performances to ignite a relationship with the land and to offer the concept of tolerance for diversity when looking at different approaches to living life. The purpose of this conference presentation is to share the experience of the …

Creep Evaluation Of (Orthotic) Cast Materials During Simulated Clubfoot Correction, T. L. Cohen, H. Altiok, Sergey Tarima, P. A. Smith, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

The Ponseti method is a widely accepted and highly successful conservative treatment of pediatric clubfoot that relies on weekly manipulations and cast applications. However, the material behavior of the cast in the Ponseti technique has not been investigated. The current study sought to characterize the ability of two standard casting materials to maintain the Ponseti corrected foot position by evaluating creep response. A dynamic cast testing device (DCTD) was built to simulate a typical pediatric clubfoot. Semi-rigid fiberglass and rigid fiberglass casting materials were applied to the device, and the rotational creep was measured at various constant torques. The movement …

Characterisation Of Friction And Lubrication Regimes In Premium Tubular Connections, F Stewart, Hr Le, Ja Williams, Et Al

School of Biological and Marine Sciences

A friction test rig has been developed to carry out repeated sliding friction tests for premium tubular connections. The test rig enables accurate measurement of friction in various contact regimes which are relevant to the threaded connections between tubular components. Higher load tests can simulate the contact in metal-to-metal seals under very high contact pressures by using perpendicular pin-on-pin tests. The contact in the thread loading flank under intermediate pressures can be simulated by using larger radius coupon-on-coupon tests. The measured coefficient of friction is well correlated with a lubrication parameter combining lubricant film thickness and initial surface roughness.

Going Deep For Drug Discovery: An Ocean To Bedside Approach To Explore Sub-Seafloor Microbes For The Next Generation Of Antibiotics, Stephanie Forschner-Dancause, L. Laplante, David C. Smith, David C. Rowley

Pharmacy Practice and Clinical Research Faculty Publications

No abstract provided.