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Kv7 K+ Channels In Airway Smooth Muscle Cells As Target For Asthma Therapy, Priyanka Prakash Kakad

Master's Theses

Airway hyperresponsiveness (AHR) is an important etiological feature of asthma, the molecular mechanisms of which are poorly understood, thus preventing development of specific and effective therapies. We investigate the role played by Kv7 voltage-activated potassium channels in the pathophysiology of AHR and evaluate the significance of their regulation in treatment of asthma. Our studies provide the first evidence for expression of multiple Kv7 channels in guinea pig and human airways and demonstrate that the expression of Kv7 channels is reduced in allergen-sensitized guinea pig airways. Our ex vivo functional studies reveal that clinically available Kv7 channel activators attenuate the bronchoconstrictor …

Agreement And Disagreement In Parent And Child Perceptions Of Spina Bifida Medical Responsibilities During The Transition To Adolescence, Alexandra Psihogios

Master's Theses

The purpose of this study was to examine the validity of informant discrepancies by evaluating levels of parent-child agreement and disagreement over who takes responsibility for SB medical tasks in relation to family conflict and medical adherence. Participants were 140 preadolescents with SB. Data used in this study are taken from the first wave of data collection (when adolescents were between the ages of 8-15) in a larger longitudinal study. Although disagreement levels predicting family conflict and medical adherence were non-significant (p > .05), a significant main effect of agreement and two significant Agreement x Conflict interactions predicting medical adherence were …

When Theory Meets Practice: Challenging Racial Inequality In A Post-Civil Rights Era, Victoria Brockett

Master's Theses

Knowledge about racial inequality is important because it can inform racially just practices. To this end, multiple scholars have shown how racial inequality operates and how it can be challenged in various facets of social life. However, what does challenging racism look like when theory meets practice? Building on racial formation theory, this thesis examines a racial justice organization's (RJO) training and consulting services through the lens of a political project that is rearticulating the meaning of race and thus, the role of race in the social structure. Evidenced by observations and interviews with RJO staff and their clients, this …

Midwife Or Med-Wife: Examining Emotion Work With Midwifery Students In Clinical Training, Jessica Anna Cebulak

Master's Theses

Midwives follow a holistic philosophy of care that goes beyond just medical intervention, providing support to both mother and family through the various stages of pregnancy and child birth. Yet, there is a lack of research in the US that examines how midwives invest emotion in their work, and the challenges they face when doing so. Drawing on the concept of Arlie Hochschild's (1979) emotion work as a lens for this study, I examine how midwifery students experience and manage emotion when delivering care to patients during clinical training in a large, urban hospital. Using eight qualitative, in-depth interviews with …

Introducing Environmental Toxicology In Instructional Labs: The Use Of A Modified Amphibian Developmental Toxicity Assay To Support Inquiry-Based Student Projects, Roger Sauterer, James R. Rayburn

Research, Publications & Creative Work

Introducing students to the process of scientific inquiry is a major goal of high school and college labs. Environmental toxins are of great concern and public interest. Modifications of a vertebrate developmental toxicity assay using the frog Xenopus laevis can support student-initiated toxicology experiments that are relevant to humans. Teams of students formulate hypotheses, perform experiments, analyze data, and present their results. By performing experiments to investigate the toxicity of household chemicals, pharmaceuticals, or agricultural chemicals, students will gain an appreciation of the environmental effects of improper disposal of common chemicals and industrial or agricultural run-off.

Zno Nanostructures: Growth, Characterization And Applications, Mikhail Ladanov

USF Tampa Graduate Theses and Dissertations

ZnO nanostructures have been investigated for quite a long time. However, only recently they triggered much interest due to advances in materials synthesis and characterization, as well as emerging demand for new nanostructured materials in novel device implementations.

A large part of the work was devoted to exploring new methodology for patterning growth sites and controlling nanowires morphology using the deposition methods that are compatible with integrated circuits (IC) processing. Microcontact printing was used to pattern the seeding layer, and, subsequently, ZnO nanowires through a resistless soft lithography process.

When considering hydrothermal growth of ZnO nanowires in the framework of …

Wetland And Lake Destruction, Development And Mental/Emotional Distress Among Residents Of Tampa Bay, Florida, Gina Larsen

USF Tampa Graduate Theses and Dissertations

The purpose of this research project is to understand how local environmental destruction in Tampa Bay, Florida, including changes in water resources and development activities, affects local Tampa Bay residents mentally and emotionally. The study also examines residents' personal connections with their landscape and documents the degree of stress that may be caused by experiencing environmental destruction through the use of interviews, freelists, and two psychometric stress scales (Hopkins Symptom Checklist-10 and the Environmental Distress Scale). The topic of emotional distress and environmental change has rarely been studied in social science research, particularly in the United States and with regards …

Linear Mixed-Effects Models: Applications To The Behavioral Sciences And Adolescent Community Health, Lizmarie Gabriela Maldonado

USF Tampa Graduate Theses and Dissertations

Linear mixed-effects (LME) modeling is a widely used statistical method for analyzing repeated measures or longitudinal data. Such longitudinal studies typically aim to investigate and describe the trajectory of a desired outcome. Longitudinal data have the advantage over cross-sectional data by providing more accuracy for the model. LME models allow researchers to account for random variation among individuals and between individuals.

In this project, adolescent health was chosen as a topic of research due to the many changes that occur during this crucial time period as a precursor to overall well-being in adult life. Understanding the factors that influence how …

Signals From Intraventricular Depth Electrodes Can Control A Brain-Computer Interface, Jerry J. Shih, Dean J. Krusienski

Electrical & Computer Engineering Faculty Publications

A Brain-Computer Interface (BCI) is a device that enables severely disabled people to communicate and interact with their environments using their brain waves. Most research investigating BCI in humans have used scalp-recorded electroencephalography (EEG). We have recently demonstrated that signals from intracranial electrocorticography (ECoG) and stereotactic depth electrodes (SDE) in the hippocampus can be used to control a BCI P300 Speller paradigm. We report a case in which stereotactic depth electrodes positioned in the ventricle were able to obtain viable signals for a BCI. Our results demonstrate that event-related potentials from intraventricular electrodes can be used to reliably control the …

Optical Detection Of Melting Point Depression For Silver Nanoparticles Via In Situ Real Time Spectroscopic Ellipsometry, S. A. Little, T. Begou, R. W. Collins, S. Marsillac

Electrical & Computer Engineering Faculty Publications

Silver nanoparticle films were deposited by sputtering at room temperature and were annealed while monitoring by real time spectroscopic ellipsometry (SE). The nanoparticle dielectric functions (0.75 eV-6.5 eV) obtained by SE were modeled using Lorentz and generalized oscillators for the nanoparticle plasmon polariton (NPP) and interband transitions, respectively. The nanoparticle melting point could be identified from variations in the oscillator parameters during annealing, and this identification was further confirmed after cooling through significant, irreversible changes in these parameters relative to the as-deposited film. The variation in melting point with physical thickness, and thus average nanoparticle diameter, as measured by SE …

Five Nuclear Loci Resolve The Polyploid History Of Switchgrass (Panicum Virgatum L.) And Relatives, Jimmy K. Triplett, Yunjing Wang, Jinshun Zhong, Elizabeth A. Kellogg

Research, Publications & Creative Work

Polyploidy poses challenges for phylogenetic reconstruction because of the need to identify and distinguish between homoeologous loci. This can be addressed by use of low copy nuclear markers. Panicum s.s. is a genus of about 100 species in the grass tribe Paniceae, subfamily Panicoideae, and is divided into five sections. Many of the species are known to be polyploids. The most well-known of the Panicum polyploids are switchgrass (Panicum virgatum) and common or Proso millet (P. miliaceum). Switchgrass is in section Virgata, along with P. tricholaenoides, P. amarum, and P. amarulum, whereas P. miliaceum is in sect. Panicum. We have …

A Multi-Year Survey Of Meiofaunal Abundance From The Northern Gulf Of Mexico Continental Shelf And Slope, Stephen C. Landers, Frank A. Romano Iii, Paul M. Stewart, Steve Ramroop

Research, Publications & Creative Work

This 3-yr (2007–09) survey documented meiofauna abundance across the northern Gulf of Mexico on the continental shelf and slope from south Texas to south Florida. Sediment samples were collected from depths ranging from 29 to 509 m (average = 132 m) on the National Oceanic and Atmospheric Admistration ship Gordon Gunter during the annual fall small pelagics fish-sampling cruise. A total of 259 sediment samples from 99 ShipekH grabs were analyzed. Meiofauna were isolated from the sediment by sieving (63-mm sieve) and concentrating the organisms via LudoxH centrifugation. Each year the two dominant animal groups were nematodes and copepods, followed …

Evolution Of Grasping Behaviour In Anthropomorphic Robotic Arms With Embodied Neural Controllers, Gianluca Massera

School of Engineering, Computing and Mathematics Theses

The works reported in this thesis focus upon synthesising neural controllers for anthropomorphic robots that are able to manipulate objects through an automatic design process based on artificial evolution. The use of Evolutionary Robotics makes it possible to reduce the characteristics and parameters specified by the designer to a minimum, and the robot’s skills evolve as it interacts with the environment. The primary objective of these experiments is to investigate whether neural controllers that are regulating the state of the motors on the basis of the current and previously experienced sensors (i.e. without relying on an inverse model) can enable …

Heart Rate And Accelerometry During Singles Footbag Net Play, Christopher Michael Siebert

Dissertations and Theses

This investigation examined the heart rate responses and movement characteristics of experienced footbag net players during singles play. Footbag net is a net/court sport similar to volleyball, but it is played with a footbag (e.g., Hacky-Sack^TM) using only the feet. In singles footbag net, players are allowed either one or two kicks to propel the footbag over the net. Subjects were 15 males and 1 female, ranging in age from 18- 60 years, with a mean age of 33.6 years. Subjects played two games of singles footbag net using two different scoring systems: "sideout" scoring and "rally" scoring. …

Sustainable Operations At Portland State University: Relevant Organizational Issues And A Path Forward, Molly Mae Bressers

Dissertations and Theses

Large organizations such as Portland State University (PSU) play an important role in the environmental impact and sustainability of a city, EcoDistrict and region. Through their resource use and operations, such organizations can assist in mitigating environmental damage, as well as educate their members and community. PSU does not currently have any formal policies that support sustainable operations, and there is room to improve the sustainability of campus operations. Feedback from PSU employees was solicited through two surveys, and these data were qualitatively analyzed to identify salient organizational issues that may serve to inhibit implementation of sustainable operations at PSU. …

On The Evolutionary Co-Adaptation Of Morphology And Distributed Neural Controllers In Adaptive Agents, Mariagiovanna Mazzapioda

School of Engineering, Computing and Mathematics Theses

The attempt to evolve complete embodied and situated artificial creatures in which both morphological and control characteristics are adapted during the evolutionary process has been and still represents a long term goal key for the artificial life and the evolutionary robotics community. Loosely inspired by ancient biological organisms which are not provided with a central nervous system and by simple organisms such as stick insects, this thesis proposes a new genotype encoding which allows development and evolution of mor- phology and neural controller in artificial agents provided with a distributed neural network. In order to understand if this kind of …

Ocean Acidification And The Loss Of Phenolic Substances In Marine Plants., Thomas Arnold, Christopher Mealey, Hannah Leahey, A. Whitman Miller, Jason M. Hall-Spencer, Marco Milazzo, Kelly Maers

School of Biological and Marine Sciences

Rising atmospheric CO(2) often triggers the production of plant phenolics, including many that serve as herbivore deterrents, digestion reducers, antimicrobials, or ultraviolet sunscreens. Such responses are predicted by popular models of plant defense, especially resource availability models which link carbon availability to phenolic biosynthesis. CO(2) availability is also increasing in the oceans, where anthropogenic emissions cause ocean acidification, decreasing seawater pH and shifting the carbonate system towards further CO(2) enrichment. Such conditions tend to increase seagrass productivity but may also increase rates of grazing on these marine plants. Here we show that high CO(2) / low pH conditions of OA …

Temperate And Tropical Brown Macroalgae Thrive, Despite Decalcification, Along Natural Co2 Gradients, Vivienne R. Johnson, Bayden D. Russell, Katharina E. Fabricius, Colin Brownlee, Jason M. Hall-Spencer

School of Biological and Marine Sciences

No abstract provided.

The Production Of Cauliflower Microshoots Using Curd Meristematic Tissues And Hypocotyl–Derived Callus., Hz Rihan, M Al-Shamari, Mp Fuller

School of Biological and Marine Sciences

The capacity for microshoot production from cauliflower was investigated applying two different protocols. In the first, cauliflower curd meristematic tissue was used as a source of explants. The meristematic layer was shaved off and the clusters produced were homogenised using a commercial blender. In terms of its effect on the number and viability of microshoots, the use of 30 s blending duration treatment was found to be optimal between several treatments tested in the range 15 to 120 sec. Explants were cultivated in agitated S23 (MS (Murashige and Skoog, 1962) + 3 % sucrose) liquid media supplemented with different combinations …

Salmon People In An Era Of Depleting Salmon: The Swinomish Indian Tribal Community’S Climate Adaptation Action Plan As A Manifestation Of Tribal Sovereignty, Kelly Davis

Tribal Law Journal

Climate change presents novel challenges to indigenous peoples striving to maintain their place-based subsistence cultures. Climate change is altering physical environments, tribes are experiencing detrimental impacts, and adaptation is necessary to preserve indigenous lifestyles. The Swinomish Indian Tribal Community, a Coast Salish tribe in northwestern Washington State, has taken the initiative in tribal climate change adaptation efforts.

In 2010, the Swinomish Office of Planning and Community Development issued a thorough Climate Adaptation Action Plan, which delineates strategies the vulnerable coastal, salmon-dependent tribe must take. Resilient social systems, like American Indian tribes, are capable of anticipating and planning for the future. …

Gull Egg Mass As A Function Of Length And Width, Melissa Mccormick

Honors Theses

The mass of a Glaucous-winged gull (Larus glaucescens) egg provides a convenient estimation of parental energy allocated towards reproduction. Obtaining accurate mass measurements, however, can be hindered by logistical constraints associated with field research. In this study, I create a mathematical model to estimate egg mass from easily determined measurements of length and width. Egg measurements were collected at a gull colony on Protection Island National Wildlife Refuge, WA. These measurements facilitated the estimation and validation of model parameters. A multi-variable allometric model incorporating both length and width variables provided the best estimation of egg mass.

The Effect Of Geographical Scale Of Sampling On Dna Barcoding., Johannes Bergsten, David T. Bilton, Tomochika Fujisawa, Miranda Elliott, Michael T. Monaghan, Michael Balke, Lars Hendrich, Joja Geijer, Jan Herrmann, Garth N. Foster, Ignacio Ribera, Anders N. Nilsson, Timothy G. Barraclough, Alfried P. Vogler

School of Biological and Marine Sciences

Eight years after DNA barcoding was formally proposed on a large scale, CO1 sequences are rapidly accumulating from around the world. While studies to date have mostly targeted local or regional species assemblages, the recent launch of the global iBOL project (International Barcode of Life), highlights the need to understand the effects of geographical scale on Barcoding's goals. Sampling has been central in the debate on DNA Barcoding, but the effect of the geographical scale of sampling has not yet been thoroughly and explicitly tested with empirical data. Here, we present a CO1 data set of aquatic predaceous diving beetles …

The Gatekeepers: Clergy Involvement In Referrals And Collaboration With Mental Health And Substance Abuse Professionals, Curtis J. Vanderwaal Dr., E. I. Hernandez, A. R. Sandman

Faculty Publications

No abstract provided.

The Role Of Protein Kinase C In The Supraesophageal Ganglion Of Female Acheta Domesticus, Sunny Kim

Honors Theses

The phonotactic response of female Acheta domesticus to the male's calling song is modulated by hormones. The presence of Juvenile Hormone III in the supraesophageal ganglion causes crickets to be more selective. This project evaluates the possibility of a second messenger system involving protein kinase C as the molecular mechanism of Juvenile Hormone III control. A solution of Juvenile Hormone III and a protein kinase C blocker was nanoinjected into the supraesophageal ganglion of nselective female crickets, causing them to remain unselective. This suggests that Juvenile Hormone III utilizes a protein kinase C-mediated second messenger system.

A Study Of Marine Benthic Algae Along A Natural Carbon Dioxide Gradient, Vivienne R Johnson

School of Biological and Marine Sciences Theses

Increasing atmospheric CO2 is causing unprecedented changes in seawater chemistry, yet the uncertainty of the ecological response to these projected changes, termed ‘ocean acidification’, remains considerable at present. To predict the effects of these changes, we need to improve our understanding of the responses of marine primary producers since these drive biogeochemical cycles and determine the structure and function of benthic habitats. The majority of experiments on the effects of ocean acidification on photoautrophs to date have mainly focused on oceanic microalgae, leaving benthic assemblages largely overlooked. Carbon dioxide vents are providing a means for examining and predicting the impacts …

Cultivating Teachers’ Morality And The Pedagogy Of Emotional Rationality, Minkang Kim

Australian Journal of Teacher Education

Teachers are expected to act ethically and provide moral role models in performing their duties, even though teacher education has often relegated the cultivation of teachers’ ethical awareness and moral development to the margins. When it is addressed, the main theoretical assumptions have relied heavily on the cognitivist developmental theories of Piaget and Kohlberg. A major pedagogical problem in adopting these theories of moral reasoning is that they may not help teachers to act as moral agents in real-life classrooms. This paper argues that one underlying difficulty is the insufficient attention given to the role of emotion in moral reasoning, …

The Utah Bioprospecting Act Of 2010: (Unintentional) State-Level Implementation Of The United Nations Convention On Biodiversity, William M. Fischer

Colorado Technology Law Journal

No abstract provided.

2012 Weed Science Research Report – Pink Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2012. It includes work on non-cropland and the management of Canada thistle and leafy spurge in North Dakota.

Correlates Of Morbidity And Mortality In Severe Necrotizing Pancreatitis, K. M. Pal, Pashtoon Murtaza Kasi, Mohammad Tayyeb, S. M. Faisal Mosharraf, Zafar Fatmi

Community Health Sciences

Acute severe pancreatitis is associated with a high morbidity and mortality and frequently is accompanied by underlying pancreatic parenchymal necrosis. Patients with pancreatic necrosis must be identified, because the morbidity and mortality rate in this subgroup is much higher. Our objective was to compare the clinical outcomes of these patients based on the degree of pancreatic necrosis. A total of 35 patients were noted to have pancreatic necrosis. These were divided into 2 groups based on extent of necrosis: group A had less than 50% necrosis and group B had more than 50% necrosis. The rate of mortality (5% versus …

Extent Of Liver Inflammation In Predicting Response To Interferon Alpha & Ribavirin In Chronic Hepatitis C Patients: A Cohort Study, Shirin Mirza, Amna Rehana Siddiqui, Saeed Hamid, Muhammad Umar, Shaheena Bashir

Community Health Sciences

Background: Liver inflammation due to HCV infection leads to fibrosis, which is an independent predictor of treatment response to interferon therapy in Chronic Hepatitis C (CHC) patients. This relationship has not been studied for liver inflammation on pretreatment liver biopsy and End of Treatment Response (ETR). ALT is a less invasive test than liver biopsy for measuring liver inflammation. Aim of this study was to compare ETR to Interferon alpha (recombinant Interferon) & Ribavirin in CHC patients having higher and lower grades of liver inflammation and to determine the diagnostic accuracy of pretreatment ALT for grades of liver inflammation.Methods: A …