Digital Commons Network^™

Synergetic Effect Of Recoverin And Calmodulin On Regulation Of Rhodopsin Kinase., Ilya I Grigoriev, Ivan I Senin, Natalya K Tikhomirova, Konstantin E Komolov, Sergei E Permyakov, Evgeni Yu Zernii, Karl-Wilhelm Koch, Pavel P Philippov

Department of Biochemistry and Molecular Biology Faculty Papers

Phosphorylation of photoactivated rhodopsin by rhodopsin kinase (RK or GRK1), a first step of the phototransduction cascade turnoff, is under the control of Ca(2+)/recoverin. Here, we demonstrate that calmodulin, a ubiquitous Ca(2+)-sensor, can inhibit RK, though less effectively than recoverin does. We have utilized the surface plasmon resonance technology to map the calmodulin binding site in the RK molecule. Calmodulin does not interact with the recoverin-binding site within amino acid residues M1-S25 of the enzyme. Instead, the high affinity calmodulin binding site is localized within a stretch of amino acid residues V150-K175 in the N-terminal regulatory region of RK. Moreover, …

Genes Adopt Non-Optimal Codon Usage To Generate Cell Cycle-Dependent Oscillations In Protein Levels., Milana Frenkel-Morgenstern, Tamar Danon, Thomas Christian, Takao Igarashi, Lydia Cohen, Ya-Ming Hou, Lars Juhl Jensen

Department of Biochemistry and Molecular Biology Faculty Papers

The cell cycle is a temporal program that regulates DNA synthesis and cell division. When we compared the codon usage of cell cycle-regulated genes with that of other genes, we discovered that there is a significant preference for non-optimal codons. Moreover, genes encoding proteins that cycle at the protein level exhibit non-optimal codon preferences. Remarkably, cell cycle-regulated genes expressed in different phases display different codon preferences. Here, we show empirically that transfer RNA (tRNA) expression is indeed highest in the G2 phase of the cell cycle, consistent with the non-optimal codon usage of genes expressed at this time, and lowest …

Impacts Of Urbanisation On The Native Avifauna Of Perth, Western Australia, Robert Davis, C Gole, Jd Roberts

Research outputs 2012

Urban development either eliminates, or severely fragments, native vegetation, and therefore alters the distribution and abundance of species that depend on it for habitat. We assessed the impact of urban development on bird communities at 121 sites in and around Perth, Western Australia. Based on data from community surveys, at least 83 % of 65 landbirds were found to be dependent, in some way, on the presence of native vegetation. For three groups of species defined by specific patterns of habitat use (bushland birds), there were sufficient data to show that species occurrences declined as the landscape changed from variegated …

Multiple Effects Of Physical Activity On Molecular And Cognitive Signs Of Brain Aging: Can Exercise Slow Neurodegeneration And Delay Alzheimer's Disease?, Belinda Brown, J J Peiffer, Ralph Martins

Research outputs 2012

Western countries are experiencing aging populations and increased longevity; thus, the incidence of dementia and Alzheimer's disease (AD) in these countries is projected to soar. In the absence of a therapeutic drug, non-pharmacological preventative approaches are being investigated. One of these approaches is regular participation in physical activity or exercise. This paper reviews studies that have explored the relationship between physical activity and cognitive function, cognitive decline, AD/dementia risk and AD-associated biomarkers and processes. There is now strong evidence that links regular physical activity or exercise to higher cognitive function, decreased cognitive decline and reduced risk of AD or dementia. …

Experimental Infection Of Malaria Mosquitoes With Entomopathogenic Fungus, Beauveria Bassiana Under Field Conditions, Annette Obukosia Busula

All Master’s Theses

Many successful laboratory studies have demonstrated the potential of using the entomopathogenic fungus, Beauveria bassiana against malaria mosquitoes. This study focussed on infection of wild malaria mosquitoes with the entomopathogeriic fungus B. bassiana under field conditions. Four experiments were carried out under laboratory conditions by use of World Health Organization bioassay cones, for exposure of3-6 days old, non-blood fed laboratory-reared Anopheles arabiensis mosquitoes on 100% black cotton cloths impregnated with spores of B. bassiana : from stocks of i) 80% viability for 10-70 minutes ii) 78, 51 and 50% including decayed spores of initially 80% viability iii) and 35% cotton …

Composition And Repellency Of Essential Oils Of Tagetes Minuta From Different Zones In Kenya Against Brown Ear Tick (Rhipicephalus Appendlculatusi, Benard Omache Makang' A

All Master’s Theses

Ticks are haematophagous ectoparasites capable of transmitting diseases to vertebrates and, therefore, constitute a threat to human, livestock and wildlife health. Though synthetic chemical aearicides have made a tremendous impact over the years in the control and management of the vector on livestock, ticks have developed resistance to most of them. In addition, the chemicals are toxic to non-target organisms. In some parts of Kenya, powdered parts of some plants, including those of Tagetes minuta L., are used to control ticks from the animals. The study aimed at characterizing and evaluating the repellency of the essential oils of T. minuta …

Economic Assessment Of Losses Due To Fruit Fly Infestation In Mango And The Willingness To Pay For An Integrated Pest Management Package In Embu District, Kenya, Caroline M. Mugure

All Master’s Theses

Mango production is a major form of income generation for Kenyan large and small-scale farmers. However, it is confronted with the major threat of fruit fly infestation which causes reduction of quality and quantity of marketable fruit resulting to considerable produce losses. New and cheaper methods to reduce fruit fly infestation levels in mango production have been developed, but farmers' willingness to pay (WTP) for them is not known. First, this study was conducted in Embu district, and it aimed to examine the magnitude of losses caused by fruit flies at the farm level via rejections during harvest using descriptive …

Optimization Of Growth Conditions Of Bacillus Thuringiensis Isolates From Various Sources In Kenya And Toxicity Assays Of Their Delta-Endotoxin Against Chilo Partellus, Daniel Anyika Kwalimwa

All Master’s Theses

Chemical pesticides have disadvantages such as high production costs, short persistence, comparative low efficacy, development of resistance to toxin and causing ecological damage. In order to obtain cheaper biopesticides, many locally available and inexpensive agricultural/industrial byproducts have potential as culture media for Bacillus thuringiensis Crystal protein production. In this study, cost-effective media were developed, based on locally available raw materials namely legumes, potato, and whey. Optimization studies indicate that pH 6.4-7.5 was best for sporulation and OD 600 was highest at 37 °C for all isolates tested. The optical density, protein concentration yield, sporulation and Chilo partellus larvicidal action were …

Apologies As Intellectual Property Remedies: Lessons From China, Xuan-Thao Nguyen

Articles

It is a frequent refrain that “the world is shrinking.” In this same vein, the global influence of China is clearly rising. Chinese businesses are becoming more prominent in the global market, and as such, the influence and effect of Chinese law is likewise gaining in import. Chinese intellectual property law is no different.

One notable aspect of Chinese intellectual property law is the availability of apology as a remedy. Despite a culture that places a high value on apology, and considerable legal scholarship and precedent regarding apology as remedy, many in the United States scoff at the notion of …

Feeling At Home: Learning, Law, Cognitive Science, And Narrative, Lea B. Vaughn

Articles

What is the "how and why" of law's affinity for narrative? In order to explain why the use of stories is such an effective teaching and presentation strategy in the law, this paper will consider theories and accounts from cognitive as well as evolutionary psychology, neuroscience, and, briefly, cultural anthropology. This account seeks to address "how" narrative helps us learn and use the law as well as "why" we are so compelled to use stories in teaching and in practice.

Brain science, simplified here, suggests that the first task is to grab someone's attention. Emotionally charged events are more likely …

A Call For Standards: An Overview Of The Current Status And Need For Guardian Standards Of Conduct And Codes Of Ethics, Karen E. Boxx, Terry W. Hammond

Articles

The role of trust in guardianships is rarely discussed, perhaps because of the assumption that court supervision of guardians reduces their power to act in any way other than trustworthy. However, as the number of persons needing guardianship protection increases while the resources available to courts to finance supervision decreases, the role of guardian is starting to become a more conventional fiduciary relationship complete with a hallmark downside-lack of supervision. Because of this trend, the concept of delineated standards for performance of a guardian's duties has taken on critical importance.

The 2001 Wingspan Conference, the second national conference on guardianship …

The Influence Of Seawater And Sulfate Reduction On Phosphate Release From Tidal Wetland Soils In The St. John’S River, Florida, Asher Williams

UNF Graduate Theses and Dissertations

Climate change and increasing sea level elevation are predicted to increase salinity in estuarine tidal wetlands in the Southeastern United States. Since much of the ecosystem function in these areas is predicated upon salinity regimes, many fundamental changes are likely to occur as a result. The influence of salinity and SO4 2- reduction on PO4 3- release from tidal wetland soils was evaluated along a salinity gradient at three sites in The St. John’s River, Florida using both field and laboratorybased methods. Porewater was sampled over the course of 10 months to determine ambient levels of SO4 2- and PO4 …

Contribution Of Spores To The Ability Of Clostridium Difficile To Adhere To Surfaces, Lovleen Tina Joshi, Daniel S. Phillips, Catrin F. Williams, Abdullah Alyousef, Les Baillie

Peninsula Dental School

ABSTRACT Clostridium difficile is the commonest cause of hospital-acquired infection in the United Kingdom. We characterized the abilities of 21 clinical isolates to form spores; to adhere to inorganic and organic surfaces, including stainless steel and human adenocarcinoma cells; and to germinate. The composition of culture media had a significant effect on spore formation, as significantly more spores were produced in brain heart infusion broth (Student's t test; P = 0.018). The spore surface relative hydrophobicity (RH) varied markedly (14 to 77%) and was correlated with the ability to adhere to stainless steel. We observed no correlation between the ribotype …

The Socio-Temporary In Architecture: Territories Of Second-Order Cybernetics, S Murrani

School of Art, Design and Architecture

No abstract provided.

Low-Cost Educational Optical Coherence Tomography System For Thickness Measurmentstomography System For Thickness Measurments, Joshua Barr, Paul V. Jansz, Steven Hinckley, Graham Wild

Research outputs 2012

We have developed an inexpensive rudimentary low coherence interferometer that can be used to measure sample thickness in the micron to mm range, and for exploring educational aspects of interferometry and optical coherence tomography.

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Probiotic Properties, Sensory Qualities, And Storage Stability Of Probiotic Banana Yogurts, Songül Çakmakçi, Bülent Çeti̇n, Tamer Turgut, Mustafa Gürses, Ahmet Erdoğan

Turkish Journal of Veterinary & Animal Sciences

The quality properties of probiotic yogurt samples made with banana marmalade (BM), which can be a probiotic product, were examined. Yogurt samples were produced from cow milk inoculated with yogurt cultures and probiotic cultures (Lactobacillus acidophilus, Bifidobacterium bifidum, and an equal mixture of the 2 strains), and then 15% BM was added to each yogurt sample. Acidity, pH, bacteria counts, and sensory analysis of the yogurt samples were investigated on days 1, 3, 5, 7, 10, and 14 during storage at 4 °C. Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, L. acidophilus, and B. bifidum counts generally decreased during the storage …

Survival And Growth Of Common Carp (Cyprinus Carpio L.) Exposed To Different Water Ph Levels, Mohammad Saeed Heydarnejad

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to examine the survival and growth performance of common carp (Cyprinus carpio L.) in water with different pH values. Carp (17.8 ± 1.21 cm; 52.14 ± 7.13 g) were transferred to 21 tanks previously adjusted with 7 different pH values: 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0. Each value had 3 replicates, and all experiments lasted for 21 days. The survival and growth of carp were assessed at days 7, 14, and 21. At the end of the 21 days, greater weight, length, survival, and biomass were found for pH values of …

Short Term Effects Of Genistein On The Testes Of Quail (Coturnix Coturnix), Meli̇ke Belkiz Erkan, Serap Eki̇nci̇

Turkish Journal of Veterinary & Animal Sciences

Although there have been a number of studies on the effects of phytoestrogens, especially genistein, on testes, its effects on the testis tissue of birds have not been studied yet. The present study aims to reveal the effects of genistein on quail testes through the use of histological and immunohistochemical methods. In the study, a total of 64 male quails (Coturnix coturnix) were used, each of which was 50 days old. Three different doses of genistein were administered by gavage to the separate groups at intervals of 15 and 30 days. According to the results of the histological analysis and …

The Influence Of Egg Shell Crack Types On Hatchability And Chick Quality, Mozhdeh Moosanezhad Khabisi, Ahmad Salahi, Seyed Naser Mousavi

Turkish Journal of Veterinary & Animal Sciences

Fertile eggs were obtained from a commercial flock of Ross broiler breeders (51 weeks old) and candled to determine the presence of hairline or star cracks. Eggs (5400 total) were assigned in equal numbers to 3 treatments (hairline-cracked, star-cracked, and normal eggs) and incubated for 21 days. Upon hatching, 10 chicks per treatment were euthanized, and organ weights were measured. Compared with normal eggs, the presence of egg shell cracks (regardless of type) resulted in higher egg weight loss at the time of transfer; a decrease in hatchability, hatch of fertile eggs, chick length, yolk-free body mass (P < 0.01), and percentage of hatchling breast and liver weight (P < 0.05); and an increase in the contamination rate of eggs (P < 0.01). Weight loss in hairline-cracked eggs was significantly higher than in star-cracked eggs (P < 0.001). Percentage hatchability and body weight of chicks hatched from star-cracked eggs was higher than in chicks from hairline-cracked eggs (P < 0.001). Crack type did not have a significant effect on chick length and body weight uniformity; however, the Pasgar score as a quality indicator was higher in chicks hatched from star-cracked eggs than those from hairline-cracked eggs (P < 0.05). There were no significant differences among treatments in terms of heart and small intestine weight. The contamination rate of hairline-cracked eggs was higher than that of star-cracked (P < 0.01). A higher incidence of embryonic mortality was observed during days 1-8 of incubation for shell-cracked eggs (P < 0.001). Total embryonic mortality in hairline-cracked, star-cracked, and normal eggs was 51.1%, 36.6%, and 13.3%, respectively. This study showed that egg shell cracks reduced incubation parameters and chick quality. The negative effects of hairline cracks were more pronounced than those of star cracks.

The Effect Of Pentylenetetrazole-Induced Seizures On Blood-Brain Barrier Integrity In A Rat Model Of Preeclampsia, Mutlu Küçük, Mehmet Kaya, Nurcan Orhan, Oğuzhan Eki̇zoğlu, Erol Arslan, Nadi̇r Arican, Rabi̇a Candan Gürses, İmdat Elmas, Ri̇vaze Kalayci, Bülent Ahishali

Turkish Journal of Veterinary & Animal Sciences

The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Methodological Tools, Ethan P. White, Xiao Xiao, J. B. Nick, Richard M. Sibly

Biology Faculty Publications

Methodological approaches for comparing metabolic theory predictions to empirical data fall into two general categories: 1) determining if the general functional form of a relationship predicted by the theory is valid; and 2) determining whether the observed values of the parameters match the specific quantitative predictions made by the theory. Both of these categories of analysis rely on being able to accurately determine the best fitting form of a model with the same general functional form as that of MTE, so we will begin by discussing how this has typically been done using OLS regression on appropriately transformed data. Potential …

Investigating Human Impact In The Environment With Faded Scaffolded Inquiry Supported By Technologies, D. T. Longhurst, M. Longhurst, A. M. Duffy, Paul G. Wolf

Biology Faculty Publications

Teaching science as inquiry is advocated in all national science education documents and by leading science and science teaching organizations. In addition to teaching science as inquiry, we recognize that learning experiences need to connect to students’ lives. This article details how we use a sequence of faded scaffolded inquiry supported by technologies to engage students meaningfully in science connected to their lives and schoolyards. In this approach, more teacher guidance is provided earlier in the inquiry experiences before this is faded later in the sequence, as students are better prepared to complete successful inquiries. The sequence of inquiry experiences …

High Tunnel Winter Spinach Production, T. Ernst, D. Drost, Brent Black

Plants, Soils, and Climate Faculty Publications

No abstract provided.

Plant Scientist Seeks Understanding Landscape Architect For Meaningful Soil-Plant-Water Relationship, Kelly L. Kopp

Plants, Soils, and Climate Faculty Publications

No abstract provided.

Evaluation Of The Possibility Of Using Oligosaccharide-Free Pea In Japanese Quail Nutrition, Danuta Szczerbinska, Zofia Tarasewicz, Piotr Gulewicz, Danuta Majewska, Marek Ligocki

Turkish Journal of Veterinary & Animal Sciences

In this paper, evaluation of the effect of processed pea seeds (free of raffinose family oligosaccharides) on the performance traits and the results of reproduction in Japanese quails is presented. A flock of 72 quails was divided into 3 groups consisting of equal numbers of birds (18 females and 6 males). Birds were kept in cages in an environmentally controlled room with a lighting cycle of 17 h of light and 7 h of dark, and they were fed 1 of 3 different diets. Whereas diet 1 served as the control, diet 2 was supplemented with 5% and diet 3 …

Molecular And Kinetic Characterization Of Lactate Dehydrogenase Enzyme In The Heart And Breast Muscle Of Japanese Quail (Coturnix Japonica), Ram Pratap Singh, Kochiganti Venkata Hanumat Sastry, Radha Agarwal, Nitin Kumar Pandey, Vishesh Kumar Saxena, Jag Mohan

Turkish Journal of Veterinary & Animal Sciences

In the present investigation, attempts were made to characterize lactate dehydrogenase (LDH) isozyme in quail heart and breast muscle by using electrophoresis, kinetic, and molecular techniques. Polyacrylamide gel electrophoresis of the heart and breast muscle revealed a single isozyme. Kinetic studies were carried out for further functional characterization of LDH isozyme in the heart and breast muscle. Substrate inhibition, enzyme kinetics, and inhibitor (urea) studies indicated that the isozymes present in these tissues differed in their functional properties. LDH isozyme present in the heart and breast muscle exhibited properties of LDH-A and LDH-B isozyme, respectively. Furthermore, RT-PCR amplification of Ldh-a …

Urinary Diversion Via Preputial Urethrostomy With Bilateral Pubic-Ischial Osteotomy In A Dog, Masaaki Katayama, Yasuhiko Okamura, Hiroaki Kamishina, Yuji Uzuka

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old, 8.7-kg intact male Pekingese dog was presented with stricture of the urethral opening after a perineal urethrostomy revision surgery. Total penile amputation and preputial urethrostomy with bilateral pubic-ischial osteotomy were performed. After surgery, the dog was able to urinate through the natural preputial orifice, avoiding urine scalding associated with urethral anastomosis to the adjacent skin. Nine months after surgery, no major complications were noted with the exception of a small amount of urine dribbling after urination.

Protective Effects Of Salvia Officinalis Extract Against Cyclophosphamide-Induced Genotoxicity And Oxidative Stress In Rats, Fulya Üstün Alkan, Feraye Esen Gürsel, Ati̇la Ateş, Mustafa Özyürek, Kubi̇lay Güçlü, Mehmet Altun

Turkish Journal of Veterinary & Animal Sciences

Medicinal plants, especially rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Salvia officinalis L., an aromatic and medicinal plant, is widely used in folk medicine and is well known for its antioxidant properties. Therefore, the present study was designed to investigate the antioxidative, possible genotoxic, and antigenotoxic potency of S. officinalis extract against cyclophosphamide (CYP)-induced oxidative stress and genotoxicity in Wistar albino rats. Animals were orally dosed with S. officinalis extract (50, 100, and 150 mg/kg body weight) for 7 days before the administration of a single intraperitoneal dose of CYP (40 mg/kg …