Digital Commons Network^™

Coxsackievirus B3 Infection Leads To The Generation Of Cardiac Myosin Heavy Chain-Α-Reactive Cd4 T Cells In A/J Mice, Arunakumar Gangaplara, Chandirasegaran Massilamany, Deborah M. Brown, Gustavo A. Delhon, Asit K. Pattnaik, Nora Chapman, Noel Rose, David J. Steffen, Jay Reddy

Jay Reddy Publications

Enteroviruses like coxsackievirus B3 (CVB3) are common suspects in myocarditis/dilated cardiomyopathy patients. Autoimmunity has been proposed as an underlying mechanism, but direct evidence of its role is lacking. To delineate autoimmune response in CVB3 myocarditis, we used IA^k dextramers for cardiac myosin heavy chain (Myhc)-α 334–352. We have demonstrated that myocarditis-susceptible A/J mice infected with CVB3 generate Myhc-α-reactive CD4 T cells and such a repertoire was absent in naïve mice as measured by proliferative response to Myhc-α 334–352 and IA^k dextramer staining. We also detected Myhc-α 334–352 dextramer⁺ cells in the hearts of CVB3-infected mice. The autoreactive …

Non-Muscle Myosin Iia Transports A Golgi Glycosyltransferase To The Endoplasmic Reticulum By Binding To Its Cytoplasmic Tail, Armen Petrosyan, Mohamed F. Ali, Shailendra Kumar Verma, Helen Cheng, Pi-Wan Cheng

United States Department of Veterans Affairs: Publications

The mechanism of the Golgi-to-ER transport of Golgi glycosyltransferases is not clear. We utilize a cell line expressing the core 2 N-acetylglucosaminyltransferase-M (C2GnT-M) tagged with c-Myc to explore this mechanism. By immunoprecipitation using anti-c-Myc antibodies coupled with proteomics analysis, we have identified several proteins including non-muscle myosin IIA (NMIIA), heat shock protein (HSP)-70 and ubiquitin activating enzyme E1 in the immunoprecipitate. Employing yeast-two-hybrid analysis and pulldown experiments, we show that the C-terminal region of the NMIIA heavy chain binds to the 1–6 amino acids in the cytoplasmic tail of C2GnT-M. We have found that NMIIA co-localizes with C2GnT-M at the …

The Relationship Between Subclinical Atherosclerosis, Non–High-Density Lipoprotein Cholesterol, Exercise, And Diet Among Male Participants Of The Pacc Project, Lauren A. Simprini, Todd C. Villines, Michael Rich, Allen J. Taylor

United States Army: Publications

BACKGROUND: Non-high-density lipoprotein (HDL) cholesterol is recommended as a secondary lipid goal treated initially with lifestyle modification. However, the relationship between non-HDL and subclinical atherosclerosis is unknown. We examined the independent relationships between coronary artery calcium (CAC), lipids including non-HDL, exercise, and diet among healthy male participants of the Prospective Army Coronary Calcium (PACC) Project.

METHODS: Male participants from the PACC Project (n = 1637, mean age 42.8 years; no history of coronary heart disease) were studied. We used validated surveys to measure dietary quality and habitual physical exercise. Fasting lipid concentrations and other cardiovascular risk variables …

Nebraska Summary: S900 Massey Ferguson 7619, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

University Of Nebraska- Lincoln Fact Book 2011-2012

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

Fact Book Table of Contents

Introduction ......................................................................................... 1

Table of Contents .......................................................................................... 2

General Information

University of Nebraska-Lincoln Core Values ................................................ 4

The Role of the University of Nebraska-Lincoln ........................................ 5

The Missions of the University of Nebraska-Lincoln ......................................... 5

Teaching...................................................................................................... 6

Research. .............................................................................. 7

Service........................................................................................... 7

Institutional and Professional Accreditations..................................................... 8

University of Nebraska-Lincoln Administrative Organization Chart ................... 11

Student Credit Hours (SCH)

Total SCH: Fall Semester Since 1979 ....................................................... 12

Total SCH: Spring Semester Since 1993 ......................................................... 13

SCH by College and Course Level, Fall and Spring Semester - 5 Year Trend ..... 14

SCH by College and …

Water Current, Volume 44, No. 1, Winter 2012

Water Current Newsletter

No abstract provided.

Effect Of Growth Promotants On The Occurrence Of Endogenous And Synthetic Steroid Hormones On Feedlot Soils And In Runoff From Beef Cattle Feeding Operations, Shannon L. Bartelt-Hunt, Daniel D. Snow, William L. Kranz, Terry L. Mader, Charles A. Shapiro, Simon Van Donk, David P. Shelton, David Tarkalson, Tian Zhang

West Central Research and Extension Center, North Platte

Supplements and growth promotants containing steroid hormones are routinely administered to beef cattle to improve feeding efficiency, reduce behavioral problems, and enhance production. As a result, beef cattle manure will contain both synthetic steroids as well as a range of endogenous steroids including androgens, estrogens, and progestogens. A two-year controlled study was conducted in which beef cattle were administered steroid hormones via subcutaneous implants and feed additives and the occurrence of 16 endogenous and synthetic steroid hormones and metabolites was evaluated in runoff from beef cattle feedlots and in manure and soil collected from feedlot surfaces. Samples were extracted and …

Ultrafast Resonance-Enhanced Multiphoton Ionization In The Azabenzenes: Pyridine, Pyridazine, Pyrimidine, And Pyrazine, Timothy D. Scarborough, David B. Foote,, Cornelis J. Uiterwaal

C.J.G.J. Uiterwaal Publications

We report on the ultrafast photoionization of pyridine, pyridazine, pyrimidine, and pyrazine. These four molecules represent a systematic series of perturbations into the structure of a benzene ring which explores the substitution of a C–H entity with a nitrogen atom, creating a heterocyclic structure. Data are recorded under intense-field, single-molecule conditions. The pulses (50 fs, 800 nm) are focused into the molecular vapor, and ion mass spectra are recorded for intensities of ∼10¹³ W/cm² to ∼10¹⁵ W/cm². We measure ion yields in the absence of the focal volume effect without the need for deconvolution of …

In Vitro Induction Of Infection-Related Hyphal Structures In Plant Pathogenic Fungi, William R Rittenour, Steven D. Harris

Department of Plant Pathology: Faculty Publications

In recent years, a voluminous amount of genomic data has been generated for several plant pathogenic fungi. Multiple studies have utilized these genomic data to advance our knowledge about the molecular mechanisms of plant pathogenesis. However, not all plant pathogenic fungi share the same infection strategies, and several genes have been identified that are crucial for plant pathogenesis in one fungus, but dispensable in others. In order for data on biological relevance to keep pace with accumulating genomic data, new biological assays need to be developed for several pathogenic fungi. Accordingly, we have developed an in vitro assay that allows …

Forest Bioenergy Feedstock Harvesting Effects On Water Supply, Daniel G. Neary, Karen A. Koestner

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Water flowing from forested catchments, both unmanaged and managed, is highly valued in terms of both quantity and quality. Increasing energy production using wood feedstocks produces varying degrees of impacts and thereby raises concerns about environmental impacts on the highly valued water supply resources of forest ecosystems. The term water supply encompasses both key components of water quantity and water quality. Water quantity considers the amount of increases or decreases, timing, consistency, and relative magnitude of water yields. Plant nutrients (anions and cations), fertilizers, herbicides, ash additions, temperature, dissolved oxygen, pH, bacteria, and sediment comprise water quality. Within the life …

Stochastic And Deterministic Processes Regulate Spatio-Temporal Variation In Seed Bank Diversity, Alejandro A. Royo, Todd E. Ristau

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Question: Seed banks often serve as reservoirs of taxonomic and genetic diversity that buffer plant populations and influence post-disturbance vegetation trajectories; yet evaluating their importance requires understanding how their composition varies within and across spatial and temporal scales (α- and β-diversity). Shifts in seed bank diversity are strongly governed by the deterministic role of differential seed longevities. Nevertheless, spatio-temporal variability in propagule depletion from and recruitment into seed banks may alter both α- and β-diversity. Here, we ask to what degree deterministic seed exhaustion, stochasticity in recruitment and mortality, or both, shape α- and β-diversity?

Location: Temperate hardwood …

Adaptive Divergence At The Margin Of An Invaded Range, Francis F. Kilkenny, Laura F. Galloway

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Invasive plant species threaten biological communities globally. However, relatively little is known about how evolutionary processes vary over the course of an invasion. To evaluate the importance of historical and adaptive drivers of range expansion, we compare the performance of North American populations of invasive Lonicera japonica from areas established 100–150 years ago, now the southern core of the range, to populations from the northern range margin, established within the last 65 years. Growth and survival of individuals from 17 core and 14 margin populations were compared in common gardens at both regions. After three years, margin plants were larger …

Identification Of Sprague's Pipit Nest Predators, Stephen K. Davis, Stephanie L. Jones, Kimberly Dohms, Teslin Holmes

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Nest predation is the primary factor influencing grassland songbird reproductive success. Understanding factors driving spatial and temporal variation in nest survival requires that we identify the primary nest predators and factors influencing predator abundance and behavior. Predation events are rarely witnessed, and the identification of nest predators is inferred, often incorrectly, from nest remains or observations of potential predators. We used video photography to identify predators of Sprague's Pipit (Anthus spragueii) nests in Saskatchewan and Montana. We monitored 60 nests in Saskatchewan and 11 nests in Montana and documented at least ten different species preying upon eggs and …

Nest Defense- Grassland Bird Responses To Snakes, Kevin Ellison, Christine Ribic

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Predation is the primary source of nest mortality for most passerines; thus, behaviors to reduce the impacts of predation are frequently quantified to study learning, adaptation, and coevolution among predator and prey species. Video surveillance of nests has made it possible to examine real-time parental nest defense. During 1999-2009, we used video camera systems to monitor 518 nests of grassland birds. We reviewed video of 48 visits by snakes to 34 nests; 37 of these visits resulted in predation of active nests. When adult birds encountered snakes at the nest (n = 33 visits), 76% of the encounters resulted …

Insights From A Synthesis Of Old And New Climate-Proxy Data From The Pyramid And Winnemucca Lake Basins For The Period 48 To 11.5 Cal Ka, Larry Benson, J.P. Smoot, S.P. Lund, S.A. Mensing, F.F. Foit Jr., R.O. Rye

United States Geological Survey: Staff Publications

A synthesis of old and new paleoclimatic data from the Pyramid and Winnemucca lake basins indicates that, between 48.0 and 11.5∙10³ calibrated years BP (hereafter ka), the climate of the western Great Basin was, to a degree, linked with the climate of the North Atlantic. Paleomagnetic secular variation (PSV) records from Pyramid Lake core PLC08-1 were tied to the GISP2 ice-core record via PSV matches to North Atlantic sediment cores whose isotopic and(or) carbonate records could be linked to the GISP2δ¹⁸O record. Relatively dry intervals in the western Great Basin were associated with cold Heinrich events and …

Laboratory Evaluation Of Large-Scale Decontamination Approaches, M. W. Calfee, S. P. Ryan, J. P. Wood, L. Mickelsen, C. Kempter, L. Miller, M. Colby, A. Touati, M. Clayton, N. Griffin-Gatchalian, S. Mcdonald, R. Delafield

United States Environmental Protection Agency: Publications

Aims: To evaluate the effectiveness of two spray-based decontamination methods for surface contamination reduction and to determine the potential for contamination spread by these methods.

Methods and Results: Material coupons (treated plywood and concrete) were contaminated with c. 1 × 10⁷ spores of Bacillus atrophaeus by aerosol deposition. Decontaminants (pH-adjusted bleach or Spor-Klenz^® RTU) were applied to coupons by either backpack sprayer or gas-powered sprayer. Contact time, reapplication frequency and rinse method were also varied. In addition to surface removal efficacy, partitioning of contamination between the rinsate and aerosol fractions was determined. Results indicated that pH-adjusted bleach …

Connecting Soil To The Cloud: A Wireless Underground Sensor Network Testbed, John Tooker, Xin Dong, Mehmet C. Vuran, Suat Irmak

School of Computing: Conference and Workshop Papers

In this demo, a novel underground communication system and an online underground sensor network testbed is demonstrated. The underground communication system, developed in the Cyber-physical Networking (CPN) Laboratory at the University of Nebraska-Lincoln, includes an underground antenna that is tailored to mitigate the adverse effects of soil on underground communication. An online connection is established with the CPN underground sensor network testbed that is located at Clay Center, Nebraska. The underground sensor network testbed consists of a network of underground communication systems equipped with soil moisture sensors and a mobile data harvesting unit equipped with cellular communication capabilities. Real-time soil …

Undergraduate And Graduate Course Descriptions, 2012 Winter, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Winter 2012.

Framing A Blaxicana Identity: A Cultural Ethnography Of Family, Race And Community In The Valley Homes, Lincoln Heights, Ohio, 1955-1960, Ana Viola Thorne

CGU Theses & Dissertations

Framing a Blaxicana Identity: A Cultural Ethnography of Family, Race and Community in the Valley Homes, Lincoln Heights, Ohio, 1955-1960 (Blaxicana Identity) is set within the construct of identity formation, against a backdrop of color and culture clash, and the social construction of race. The author's narrative will constitute contextual introductions to discussion topics and iterate direct correlations of her lived experience to larger community and cultural accounts that helped to shape aspects of her Blaxicana identity. The individual and community perceptions of what it means and what it feels like to grow up Negro, Mexican and female in an …

Optimization And Development Of In Vitro Bioassays To Determine Structure-Activity Relationships For Cannabinoid Receptor 1 And Cannabinoid Receptor 2, Afeef Husni

Electronic Theses and Dissertations

Cannabis has been around for thousands of years and has been used recreationally, medicinally, and for fiber. Over 400 compounds have been isolated from Cannabis sativa with approximately 100 being cannabinoids. Of those 100 compounds, Δ9-THC has been determined as the primary constituent, which is also responsible for the psychoactivity associated with Cannabis. Along with Δ9-THC, cannabidiol has been studied in depth for its therapeutic effects. This has currently been the focus of many researchers since cannabidiol does not cause psychotropic effects.

Cannabinoid receptors belong to the large superfamily of G protein-coupled receptors (GPCRs). Approximately 30% of marketed drugs target …

Pharmaceutical Formulation Development Utilizing Hot Melt Extrusion And Other Techniques For Development Of Immediate And Controlled Release Dosage Forms, Noorullah Naqvi Mohammed

Electronic Theses and Dissertations

Hot melt extrusion (HME) was evaluated as a processing technology for the manufacture of immediate as well as controlled release formulations for oral delivery of two model compounds. For immediate release applications lower molecular weight grades of Hydroxypropyl cellulose polymers, EF and ELF, were utilized as carrier matrices to form solid solutions of a poorly soluble compound, Ketoprofen (KPR). Thermal characterization techniques were used to confirm thermal stability, miscibility and setting up processing conditions for extrusion. Extruded matrices were pelletized to be filled into pellets or further milled and compressed into tablets. Pellets exhibited a carrier dependent release with ELF …

Recent Advances In The Application Of Non Thermal Methods For The Prevention Of Salmonella In Foods, Shilpi Gupta, Nissreen Abu-Ghannam

Books/Book Chapters/ Proceedings

Food-borne illness as a result of consumption of foods contaminated with pathogenic bacteria is a world-wide concern. The presence and subsequent growth of micro-organisms in food in addition to improper storage not only results in spoilage but also in a reduction of food quality. The microbiological safety in ready to eat products is a cause of big concern not only for the consumers and food industries but also for the regulatory agencies. The number of documented outbreaks of foodborne diseases has increased in the last decade with Salmonella spp., Listeria monocytogenes and Escherichia coli being responsible for the largest number …

The Rollamo 2012, Missouri University Of Science And Technology

Yearbooks

No abstract provided.

Graduate Showcase 2012: Education For The Future, The Graduate School, Grand Valley State University

Graduate Showcase Previous Years

No abstract provided.

2012 Presentation Abstracts, Grand Valley State University

Academic Conference Fund Abstracts

No abstract provided.

The Grammar Of Context: Breakfast, Bumper Stickers, And Beyond, Cheryl Almeda, Jonathan Bush

Language Arts Journal of Michigan

No abstract provided.

Pheromonal Interactions Among Gametophytes Of Osmundastrum Cinnamomeum And The Origins Of Antheridiogen Systems In Leptosporangiate Ferns, Stephanie N. Hollingsworth, Eric A. Andres, Gary K. Greery

Peer Reviewed Publications

Antheridiogen systems, whereby notch-bearing, archegoniate gametophytes induce maleness in ameristic neighbors, have been detected in many core leptosporangiate ferns. Previous studies have failed to detect an antheridiogen system in Osmundales, which is sister to all other extant leptosporangiates; hence, antheridiogen systems are thought to have evolved after their divergence. Detailed studies of morphological development and patterns of gender expression in Osmundales and other basal leptosporangiate clades are needed to clarify how antheridiogen systems evolved. Here, we tracked the development and gender expression of gametophytes of Osmundastrum cinnamomeum grown in isolation and multispore populations exposed to basal media (control), gibberellic acid …

Estimated Change In Tundra Ecosystem Function Near Barrow, Alaska Between 1972 And 2010, M. J. Lara, S. Villarreal, D. R. Johnson, R. D. Hollister, P. J. Webber, C. E. Tweedie

Peer Reviewed Publications

How the greening of Arctic landscapes manifests as a change in ecosystem structure and function remains largely unknown. This study investigates the likely implications of plant community change on ecosystem function in tundra near Barrow, Alaska. We use structural data from marked plots, established in 1972 and resampled in 1999, 2008 and 2010 to assess plant community change. Ecosystem functional studies were made close to peak growing season in 2008 and 2010 on destructive plots adjacent to marked plots and included measurement of land–atmosphere CH4 and CO2 exchange, hyperspectral reflectance, albedo, water table height, soil moisture, and plant species cover …

Tundra Vegetation Change Near Barrow, Alaska (1972–2010), S. Villarreal, R. D. Hollister, D. R. Johnson, M. J. Lara, P. J. Webber, C. E. Tweedie

Peer Reviewed Publications

Knowledge of how arctic plant communities will respond to change has been largely derived from plot level experimental manipulation, not from trends of decade time scale environmental observations. This study documents plant community change in 330 marked plots at 33 sites established during the International Biological Program near Barrow, Alaska in 1972. Plots were resampled in 1999, 2008 and 2010 for species cover and presence. Cluster analysis identified nine plant communities in 1972. Non-metric multidimensional scaling (NMS) indicates that plant communities have changed in different ways over time, and that wet communities have changed more than dry communities. The relative …

Rhododendron Species In The Indian Eastern Himalayas: New Approaches To Understanding Rare Plant Species Distributions, Shaily Menon, Mohamed Latif Khan, Ashish Paul, A. Townsend Peterson

Peer Reviewed Publications

Rhododendrons are an important, dominant, and primitive group of flowering plants with considerable ecological and economic importance found in the temperate, subalpine and alpine regions of western Arunachal Pradesh, India. In addition to aesthetic, sacred, and ethnomedicinal values, several species have commercial and social importance. Rhododendrons are one of the preferred plant species used by local inhabitants in the region. Anthropogenic disturbance associated with deforestation, unsustainable extraction, over-exploitation, and agricultural practices have collectively put pressure on Rhododendron species; as a result many species have become endangered, rare, or threatened. Knowledge of the specifics of a species’ distribution is essential for …