Digital Commons Network^™

Structure-Function Studies Of Dna Binding Domain Of Response Regulator Kdpe Reveals Equal Affinity Interactions At Dna Half-Sites., Anoop Narayanan, Lak N. Paul, Sakshi Tomae, Dipak N. Patil, Pravindra Kumar, Dinesh A. Yernool

Department of Biological Sciences Faculty Publications

Expression of KdpFABC, a K+ pump that restores osmotic balance, is controlled by binding of the response regulator KdpE to a specific DNA sequence (kdpFABCBS) via the winged helix-turn-helix type DNA binding domain (KdpEDBD). Exploration of E. coli KdpEDBD andkdpFABCBS interaction resulted in the identification of two conserved, AT-rich 6 bp direct repeats that form half-sites. Despite binding to these half-sites, KdpEDBD was incapable of promoting gene expression in vivo. Structure-function studies guided by our 2.5 Å X-ray structure of KdpEDBD revealed the importance of residues R193 and R200 in the α-8 DNA recognition helix and …

Expression Of 14-3-3 Protein Isoforms In Mouse Oocytes, Eggs And Ovarian Follicular Development, Santanu De, Jennifer Marcinkiewicz, Srinivasan Vijayaraghavan, Douglas Kline

Biology Faculty Articles

Background

The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins. Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and, σ). These proteins associate with many intracellular proteins involved in a variety of cellular processes including regulation of the cell cycle, metabolism and protein trafficking. We are particularly interested in the role of 14-3-3 in meiosis in mammalian eggs and the role 14-3-3 proteins may play in ovarian function. Therefore, we examined the expression of 14-3-3 proteins in mouse oocyte and egg extracts by Western blotting after polyacrylamide gel electrophoresis, viewed fixed …

Transfer Pricing: Data Dumps And Comparability — Studies From The U.S., U.K., Canada, And Australia, Richard Thompson Ainsworth, Andrew B. Shact

Faculty Scholarship

Comparability is the heart of transfer pricing. The OECD, U.K., Canadian, Australian, and U.S. transfer pricing rules all echo one another on how critically important the comparability analysis is. Performing this analysis and proving comparability, however, is a demanding exercise.

What makes proving comparability so difficult is that the analysis is two sided. Both controlled and uncontrolled transactions must be thoroughly analyzed. Just as much effort needs to be applied to determine the functions, contract terms, risks and the economic conditions for the unrelated party comparables as is spent on analyzing the related parties (taxpayers).

But there is more to …

Optimal Population Of Foxp3+ T Cells In Tumors Requires An Antigen Priming-Dependent Trafficking Receptor Switch., Chuanwu Wang, Jee H. Lee, Chang H. Kim

Department of Comparative Pathobiology Faculty Publications

FoxP3+ T cells populate tumors and regulate anti-tumor immunity. The requirement for optimal population of FoxP3+ regulatory T cells in tumors remains unclear. We investigated the migration requirement and stability of tumor-associated FoxP3+ T cells. We found that only memory, but not naïve, FoxP3+ T cells are highly enriched in tumors. Almost all of the tumor-infiltrating FoxP3+ T cells express Helios, an antigen associated either with thymus-generated FoxP3+ T cells or activated T cells in the periphery. The tumor-infiltrating FoxP3+ T cells largely lack CD62L and CCR7, two trafficking receptors required for T cell migration into secondary lymphoid tissues. Instead, …

Glenohumeral Tuberculous Arthritis Complicated With Beta Haemolytic Streptococcus: An Extraordinary Rare Association: A Case Report, Rizwan Haroon Rashid, Muhammad Usman Sarwar, Jaweed Akhter, Shahryar Noordin

Section of Orthopaedic Surgery

Introduction: Septic arthritis of the glenohumeral joint is a rare entity and its diagnosis is difficult with a superadded infection in the presence of underlying tuberculosis. We report the first case of group B beta haemolytic streptococcal glenohumeral arthritis with underlying tuberculosis.
Case Presentation: A 40 year old lady previously diagnosed to have poliomyelitis, rheumatoid arthritis, hepatitis C, and diabetes mellitus for the last 10 years, presented to the emergency room with diabetic ketoacidosis. Two weeks prior to presentation she developed fever along with pain and swelling in left shoulder with uncontrolled blood sugars. Local examination of the shoulder revealed …

Genome-Wide Analysis Of Syntenic Gene Deletion In The Grasses, James C. Schnable, Michael Freeling, Eric Lyons

Department of Agronomy and Horticulture: Faculty Publications

The grasses, Poaceae, are one of the largest and most successful angiosperm families. Like many radiations of flowering plants, the divergence of the major grass lineages was preceded by a whole-genome duplication (WGD), although these events are not rare for flowering plants. By combining identification of syntenic gene blocks with measures of gene pair divergence and different frequencies of ancient gene loss, we have separated the two subgenomes present in modern grasses. Reciprocal loss of duplicated genes or genomic regions has been hypothesized to reproductively isolate populations and, thus, speciation. However, in contrast to previous studies in yeast and teleost …

Epigenetic Regulation Of Genes Involved In Vascular Dysfunction In Preeclamptic Women, Ahmad Mousa

Theses and Dissertations

DNA methylation is the most recognizable epigenetic mechanism. In general, DNA hypomethylation is associated with increased gene expression whereas DNA hypermethylation is associated with decreased gene expression. To date, little is known about the role of DNA methylation in the pathophysiology of preeclampsia. In this study, we examined the differences in DNA methylation in omental arteries of normal pregnant and preeclamptic women using the high throughput Illumina HumanMethylation27 BeadChip assay. We found 1,685 genes with a significant difference in DNA methylation at a false discovery rate of < 10% with many inflammatory genes having reduced methylation. The thromboxane synthase gene was the most hypomethylated gene in preeclamptic women as compared to normal pregnant women. When we examined the expression of thromboxane synthase in omental arteries of normal pregnant and preeclamptic women we found it to be significantly increased in preeclamptic women. The increased expression was observed in vascular smooth muscle cells, endothelial cells and infiltrating neutrophils. Experimentally induced DNA hypomethylation increased the expression of thromboxane synthase in the neutrophil-like HL-60 cell line, whereas tumor necrosis factor α (TNFα), a neutrophil product, increased its expression in cultured human vascular smooth muscle cells (VSMC). These finding suggest that DNA methylation and release of TNFα by infiltrating neutrophils could contribute to the increased expression of thromboxane synthase in systemic blood vessels of preeclamptic women, contributing to the hypertension and coagulation abnormalities. We also explored the possible contribution of DNA methylation to the altered expression of genes involved in collagen metabolism in preeclampsia. Several matrix metalloproteinase (MMP) genes, including MMP1 and MMP8, were significantly less methylated in preeclamptic women, whereas TIMP and COL genes were either significantly more methylated or had no significant change in their DNA methylation status. Experimentally induced DNA hypomethylation increased the expression of MMP-1, but not TIMP-1 or COL1A1, in cultured VSMCs and increased the expression of MMP-1 and MMP-8 in HL-60 cells. These findings suggest that DNA methylation contributes to the imbalance in genes involved in collagen metabolism in blood vessels of preeclamptic women.

University Of Northern Iowa Faculty Senate Meeting Minutes, January 23, 2012, University Of Northern Iowa. Faculty Senate.

Documents - Faculty Senate

Meeting minutes from the Faculty Senate of the University of Northern Iowa.

Sbe Notícias, Ano 7, No. 215, January 21, 2012, Marcelo A. Rasteiro, Delci K. Ishida, Agatha Matsumoto

SBE Notícias

Associado da SBE é eleito chairman da ISCA; Nossa História; Foto revela beleza de cavernas em maior geleira da Europa; Robô consegue voar como morcego; Vida na caverna para escapar de radiação eletromagnética; CECAV seleciona gestor para Chapada Diamantina; 46º CBG inicia chamada de trabalhos; Estudo avalia a dinâmica de recursos alimentares de uma caverna em Goiás; Foto do Leitor: Gruta dos Paiva (SP-42)

“It’S Just Like The Resurrection”: The Boer Surrender To The Canadians At Paardeberg, Cameron Pulsifer

Canadian Military History

No abstract provided.

Science, Math, Social Studies, And Language Arts Achievement Of High School Students In Complete Programs Of Agriscience Education, Dennis W. Duncan, John C. Ricketts, Todd Shultz

Online Journal for Workforce Education and Development

The purpose of this descriptive and comparative study was to describe the academic achievement of FFA (Future Farmers of America) members in a complete program of agriscience at Jackson County Comprehensive High School (JCCHS). A secondary purpose was to compare science, math, social studies, and language arts achievement of senior agriscience students/FFA members to the achievement of all seniors at JCCHS. The findings indicate that senior agriscience students/FFA members had a higher percentage of students achieving at the highest level (Pass Plus) of Language Arts on the GHSGT (Georgia High School Graduation Test) than the remaining student population at JCCHS. …

A Postmodern International Business Communication Model In Three-Dimensions, Jane Thompson Johansen

Global Advances in Business Communication

Representational models of communication behavior have been created in the United States to describe specific communication problems since the early 1940’s when communication science was recognized as a separate social science. The three-dimensional model presented here describes the intricacies of 21st century international business communication. Its creation rests on three requirements demanded by current communication speed and technical communication capabilities. First, the model treats culture as a characteristic of communicators. Second, it discusses the dynamism or changes in communicators and the message itself. Third, it also describes the education of international communicators as they recontextualize their cultures to match each …

Nbc's Tom Brokaw Returns To Dominican Campus, Sarah Gardner, Dave Albee

Press Releases

Brokaw, former Michigan Governor Jennifer Granholm, and authors Eve Ensler, Isabel Allende, Anne Lamott, and Robert Bellah, are headlining Dominican's ILS Lecture Series this winter and spring.

Volume 47 - Issue 14 - Friday, January 20, 2012, Rose Thorn Staff

The Rose Thorn Archive

The Rose Thorn, Rose-Hulman's independent student newspaper.

Spacial Ecology, Behavior, And Habitat Use Of Terrestrial Coastal Giant Salamanders (Dicamptodon Tenebrosus) In The Central Washington Cascades, Brandon Fessler

Master of Biology Theses

I used radio-telemetry and remote camera monitoring ot investigate the terrestrial ecology Coastal Giant Salamanders (Dicamptodon tenebrosus). I tracked twelve salamanders an average of 319 days (range: 85-470) between June 2010 and September 2011. The majority of movements between tracking sessions were <20 m(85%, n= 209). The maximum movement distance observed was 271 m over three days. Camera monitoring showed D. tenebrosus move nocturnally, typically between 1900h and 0500h. Upland habitat use was common for D. tenebrosus, although all animals remained within 50 mof streams or seeps. Salamanders exhibited both range and refuge site fidelity; animals returned to previously occupied locations with wide variation ni the time (range: 6-381 days) and total distance traveled (range: 3-259 m) since last occupation. Salamanders were visible (both day and night) during 33.8% of radio-telemetry and 56.5% of camera monitoring sessions, typically seen facing out of refuge entrances.

Winter 2012 First Year Ra Training On Lgbtiqq Issues, Annie Russell

Lesbian Gay Bisexual Transgender Queer Center

This is the Winter 2012 First Year RA Training on LGBTIQQ Issues presented by Annie Russell.

January 20, 2012, Ohio University Board Of Trustees Meeting Minutes, Ohio University Board Of Trustees

Board of Trustees Meeting Minutes

Meeting minutes document the activities of Ohio University's Board of Trustees.

Pressure-Overload-Induced Subcellular Relocalization/Oxidation Of Soluble Guanylyl Cyclase In The Heart Modulates Enzyme Stimulation., Emily J Tsai, Yuchuan Liu, Norimichi Koitabashi, Djahida Bedja, Thomas Danner, Jean-Francois Jasmin, Michael P Lisanti, Andreas Friebe, Eiki Takimoto, David A Kass

Department of Stem Cell Biology and Regenerative Medicine Faculty Papers & Presentations

RATIONALE: Soluble guanylyl cyclase (sGC) generates cyclic guanosine monophophate (cGMP) upon activation by nitric oxide (NO). Cardiac NO-sGC-cGMP signaling blunts cardiac stress responses, including pressure-overload-induced hypertrophy. The latter itself depresses signaling through this pathway by reducing NO generation and enhancing cGMP hydrolysis.

OBJECTIVE: We tested the hypothesis that the sGC response to NO also declines with pressure-overload stress and assessed the role of heme-oxidation and altered intracellular compartmentation of sGC as potential mechanisms.

METHODS AND RESULTS: C57BL/6 mice subjected to transverse aortic constriction (TAC) developed cardiac hypertrophy and dysfunction. NO-stimulated sGC activity was markedly depressed, whereas NO- and heme-independent sGC …

Volume Cxxix, Number 11, January 20, 2012, Lawrence University

The Lawrentian

No abstract provided.

Charge-Based Interaction Conserved Within Histone H3 Lysine 4 (H3k4) Methyltransferase Complexes Is Needed For Protein Stability, Histone Methylation, And Gene Expression, Douglas P. Mersman, Hai Ning Du, Ian M. Fingerman, Paul F. South, Scott D. Briggs

Faculty Publications

Histone H3 lysine 4 (H3K4) methyltransferases are conserved from yeast to humans, assemble in multisubunit complexes, and are needed to regulate gene expression. The yeast H3K4 methyltransferase complex, Set1 complex or complex of proteins associated with Set1 (COMPASS), consists of Set1 and conserved Set1-associated proteins: Swd1, Swd2, Swd3, Spp1, Bre2, Sdc1, and Shg1. The removal of the WD40 domain-containing subunits Swd1 and Swd3 leads to a loss of Set1 protein and consequently a complete loss ofH3K4methylation. However, until now, how these WD40 domain-containing proteins interact with Set1 and contribute to the stability of Set1 and H3K4 methylation has not been …

Rna-Seq Based Transcriptional Map Of Bovine Respiratory Disease Pathogen "Histophilus Somni 2336"., Ranjit Kumar, Mark L. Lawrence, James Watt, Amanda M. Cooksey, Shane C. Burgess, Bindu Nanduri

CVM Publications

Genome structural annotation, i.e., identification and demarcation of the boundaries for all the functional elements in a genome (e.g., genes, non-coding RNAs, proteins and regulatory elements), is a prerequisite for systems level analysis. Current genome annotation programs do not identify all of the functional elements of the genome, especially small non-coding RNAs (sRNAs). Whole genome transcriptome analysis is a complementary method to identify "novel" genes, small RNAs, regulatory regions, and operon structures, thus improving the structural annotation in bacteria. In particular, the identification of non-coding RNAs has revealed their widespread occurrence and functional importance in gene regulation, stress and virulence. …

Kenyon Collegiate - January 20, 2012

Kenyon Collegiate

No abstract provided.

The Daily Gamecock, Friday, January 20, 2012, University Of South Carolina, Office Of Student Media

January

No abstract provided.

Minutes, College Of Arts & Sciences Academic Programs Committee Meeting, January 20, 2012, University Of South Florida St. Petersburg. College Of Arts And Sciences

Academic Programs Committee Meeting Minutes

Minutes from the January 20, 2012, meeting of the University of South Florida St. Petersburg College of Arts & Sciences's Academic Programs Committee.

Desiccation Induces Viable But Non-Culturable Cells In Sinorhizobium Meliloti 1021, Jan A.C. Vriezen, Frans J. De Bruijn, Klaus R. Nüsslein

Biological Sciences: Faculty Publications

Sinorhizobium meliloti is a microorganism commercially used in the production of e.g. Medicago sativa seed inocula. Many inocula are powder-based and production includes a drying step. Although S. meliloti survives drying well, the quality of the inocula is reduced during this process. In this study we determined survival during desiccation of the commercial strains 102F84 and 102F85 as well as the model strain USDA1021.

The survival of S. meliloti 1021 was estimated during nine weeks at 22% relative humidity. We found that after an initial rapid decline of colony forming units, the decline slowed to a steady 10-fold reduction …

Injury Risk At Work, Safety Motivation, And The Role Of Masculinity: A Moderated Mediation, Timothy J. Bauerle

Master's Theses

No abstract provided.

Heterogeneity In Mitochondrial Morphology And Membrane Potential Is Independent Of The Nuclear Division Cycle In Multinucleate Fungal Cells, John P. Gerstenberger, Patricia Occhipinti, Amy S. Gladfelter

Dartmouth Scholarship

In the multinucleate filamentous fungus Ashbya gossypii, nuclei divide asynchronously in a common cytoplasm. We hypothesize that the division cycle machinery has a limited zone of influence in the cytoplasm to promote nuclear autonomy. Mitochondria in cultured mammalian cells undergo cell cycle-specific changes in morphology and membrane potential and therefore can serve as a reporter of the cell cycle state of the cytoplasm. To evaluate if the cell cycle state of nuclei in A. gossypii can influ

Likelihood Of Occurrence Of Bark Beetle Attacks On Conifer Forests In Honduras Under Normal And Climate Change Scenarios, Alexander J. Hernandez, Javier Saborio, R. Douglas Ramsey, Samuel Rivera

Wildland Resources Faculty Publications

Conifer forests cover approximately 27% of Honduras (∼3 million ha), and have been traditionally affected by bark beetle (Dendroctonus frontalis) outbreaks. These outbreaks impact ecosystem health and predispose more attacks. We developed a logistic model to assess the forests’ susceptibility to a beetle attack. Models were fitted using climatic, topographic and remote sensing variables. We show a method to generate pseudo-absences based on a multi-temporal wetness index. Our optimized threshold to convert the continuum of probabilities into 0 and 1 value was 0.65. Our overall accuracy was 68.7%. We also developed models that integrate climate change scenarios. Our …

Remembering President Evelyn Handler, Lois Shea

UNH Today Archive

No abstract provided.

Statistical Methods For Normalization And Analysis Of High-Throughput Genomic Data, Tobias Guennel

Theses and Dissertations

High-throughput genomic datasets obtained from microarray or sequencing studies have revolutionized the field of molecular biology over the last decade. The complexity of these new technologies also poses new challenges to statisticians to separate biological relevant information from technical noise. Two methods are introduced that address important issues with normalization of array comparative genomic hybridization (aCGH) microarrays and the analysis of RNA sequencing (RNA-Seq) studies. Many studies investigating copy number aberrations at the DNA level for cancer and genetic studies use comparative genomic hybridization (CGH) on oligo arrays. However, aCGH data often suffer from low signal to noise ratios resulting …