Digital Commons Network^™

Allergen Sensitization And Immunomodulation By Synthetic Ligands, Pul-042, In An Allergen-Induced Asthma Murine Model, Maryann A. Edwards

Dissertations and Theses (Open Access)

Allergen-induced asthma is the leading form of asthma and a chronic condition worldwide. Common allergens are known to contribute to the pathogenesis of this disease. Murine models of allergic asthma have mostly used an intraperitoneal route of sensitization (not airway) to study this disease. Allergic asthma pathophysiology involves the activation of T_H2-specific cells, which triggers production of IgE antibodies, the up-regulation of T_H2-specific cytokines (i.e. IL-4, IL-5, IL-9 and IL-13), increased airway eosinophilia, and mucin hypersecretion. Although there are several therapeutics currently treating asthmatic patients, some of these treatments can result in drug tolerance and …

Patterns Of Diseases And Preventive Measures Among Domestic Hajjis From Central, Saudi Arabia, Fahad S. Al-Jasser, Ibrahim A. Kabbash, Mohammad A. Almazroa, Ziad A. Memish

Saudi Medical Journal

OBJECTIVE: To identify the occurrence of upper respiratory tract infections (URTI), diarrheal diseases and trauma during the Hajj season, and the practice of some preventive measures by pilgrims. METHODS: A cross-sectional study during November and December 2009 among hajjis registered while visiting Primary Health Care Centers of Riyadh, Kingdom of Saudi Arabia to get mandatory meningococcal meningitis vaccination. On return from hajj, hajjis were contacted on telephone to collect information on occurrence of URTI and diarrhea along with other associated activities in Hajj. RESULTS: Out of 1507 hajjis, 54.7% developed symptoms; 97% reported upper respiratory tract symptoms, and 9.3% reported …

Leptospiral Hemolysins Induce Proinflammatory Cytokines Through Toll-Like Receptor 2-And 4-Mediated Jnk And Nf-Κb Signaling Pathways, Huan Wang, Yifei Hu, David M. Ojcius, X. Frank Yang, Chenglin Zhang, Shibiao Ding, Xu'ai Lin, Jie Yan

All Dugoni School of Dentistry Faculty Articles

Infection with pathogenic Leptospira species causes serious systemic inflammation in patients. Although a few leptospiral proinflammatory molecules have been identified, Leptospira likely encodes other unidentified strong inflammation stimulators. The pathogenic L. interrogans genome encodes numerous putative hemolysin genes. Since hemolysins from other bacteria can cause inflammatory reactions, we hypothesized that leptospiral hemolysins may function as proinflammatory stimulators that contribute to the strong inflammation associated with Leptospira infection.

Thy Brother’S Keeper? The Relationship Between Social Distance And Intensity Of Dehumanization During Genocide, Lisa Haagensen, Marnix Croes

Genocide Studies and Prevention: An International Journal

This paper puts forth the hypothesis that the degree of social distance between perpetrator and vic- tim groups prior to the outbreak of genocide is inversely related to the degree of severity of dehu- manization employed by the perpetrator group during genocide. Derived from psychological theory, this hypothesis is illustrated by using a primarily literature-based method of analysis combined with a vignette-designed severity of dehumanization scale. Three genocides are compared: the Rwandan Genocide, the Holocaust as it occurred in Western Europe, and the Holocaust as it occurred in East- ern Europe. The findings for Rwanda and the Holocaust in Eastern …

Gene Expression In Intestinal Mucosal Biopsy Specimens Obtained From Dogs With Chronic Enteropathy, Vicki L. Wilke, Dan Nettleton, Meghan J. Wymore, Jack M. Gallup, Cumhur Yusuf Demirkale, Mark R. Ackermann, Chris K. Tuggle, Amanda E. Ramer-Tait, Michael J. Wannemeuhler, Albert E. Jergens

Department of Food Science and Technology: Faculty Publications

Objective—To characterize mucosal gene expression in dogs with chronic enteropathy (CE). Animals—18 dogs with CE and 6 healthy control dogs.

Procedures—Small intestinal mucosal biopsy specimens were endoscopically obtained from dogs. Disease severity in dogs with CE was determined via inflammatory bowel index scores and histologic grading of biopsy specimens. Total RNA was extracted from biopsy specimens and microchip array analysis (approx 43,000 probe sets) and quantitative reverse transcriptase PCR assays were performed.

Results—1,875 genes were differentially expressed between dogs with CE and healthy control dogs; 1,582 (85%) genes were downregulated in dogs with CE, including neurotensin, fatty …

Melanopsin Mediates Retrograde Visual Signaling In The Retina, Dao-Qi Zhang, Michael A. Belenky, Patricia J. Sollars, Gary E. Pickard, Douglas G. Mcmahon

School of Veterinary and Biomedical Sciences: Faculty Publications

The canonical flow of visual signals proceeds from outer to inner retina (photoreceptors→bipolar cells→ganglion cells). However, melanopsin-expressing ganglion cells are photosensitive and functional sustained light signaling to retinal dopaminergic interneurons persists in the absence of rods and cones. Here we show that the sustained-type light response of retinal dopamine neurons requires melanopsin and that the response is mediated by AMPA-type glutamate receptors, defining a retrograde retinal visual signaling pathway that fully reverses the usual flow of light signals in retinal circuits.

Hemolytic Anemia In An Immuno-Competent Infant Due To Acute Cytomegalovirus Infection, Mohamed A. Hendaus

Saudi Medical Journal

No abstract provided.

13C Nmr Reveals No Evidence Of N–Π* Interactions In Proteins, Bradley Worley, Georgia Richard, Gerard S. Harbison, Robert Powers

Department of Chemistry: Faculty Publications

An n ‒ Π* interaction between neighboring carbonyl groups has been postulated to stabilize protein structures. Such an interaction would affect the ¹³C chemical shielding of the carbonyl groups, whose paramagnetic component is dominated by n ‒ Π* and Π ‒ Π* excitations. Model compound calculations indicate that both the interaction energetics and the chemical shielding of the carbonyl group are instead dominated by a classical dipole-dipole interaction. A set of high resolution protein structures with associated carbonyl ¹³C chemical shift assignments verifies this correlation and provides no evidence for an inter-carbonyl n ‒ Π* interaction.

Panic Behind The Mask: The Spanish Influenza Epidemic Of 1918 In New Orleans, Sarah Theresa Savage

Master's Theses

As part of the most devastating influenza pandemic in modern history, the Spanish Influenza epidemic in New Orleans left the city emotionally and physically crippled as residents struggled to resume daily life after thousands succumbed to a bloody cough and painful death in October 1918. When New Orleans public health officials reacted to the explosion of Spanish Influenza cases on October 10, 1918, the virus had already traveled throughout the population. Unlike previous influenza outbreaks, the 1918 epidemic killed primarily young healthy adults, the backbones of the working force and families. In an attempt to quarantine the ill from the …

Il-22 Signaling Contributes To West Nile Encephalitis Pathogenesis, Fengwei Bai, Penghua Wang, Lauren A. Zenewicz, Jianfeng Dai, David Gate, Gong Cheng, Long Yang, Feng Qian, Xiaoling Yuan, Ruth R. Montgomery, Richard A. Flavell, Terrence Town, Erol Fikrig

Faculty Publications

The Th17 cytokine, IL-22, regulates host immune responses to extracellular pathogens. Whether IL-22 plays a role in viral infection, however, is poorly understood. We report here that Il22^-/- mice were more resistant to lethal West Nile virus (WNV) encephalitis, but had similar viral loads in the periphery compared to wild type (WT) mice. Viral loads, leukocyte infiltrates, proinflammatory cytokines and apoptotic cells in the central nervous system (CNS) of Il22^-/- mice were also strikingly reduced. Further examination showed that Cxcr2, a chemokine receptor that plays a non-redundant role in mediating neutrophil migration, was significantly reduced in Il22^{-/- …}

Synthetic Biology: Does Re-Writing Nature Require Re-Writing Regulation?, Jordan Paradise, Ethan Fitzpatrick

Penn State Law Review (2003 - Present)

No abstract provided.

Regulation Of To Xin Synthesis By Clostridium Difficile, Charles Darkoh

Dissertations and Theses (Open Access)

Clostridium difficile is the leading definable cause of nosocomial diarrhea worldwide due to its virulence, multi-drug resistance, spore-forming ability, and environmental persistence. The incidence of C. difficile infection (CDI) has been increasing exponentially in the last decade. Virulent strains of C. difficile produce either toxin A and/or toxin B, which are essential for the pathogenesis of this bacterium. Current methods for diagnosing CDI are mostly qualitative tests that detect the bacterium, the toxins, or the toxin genes. These methods do not differentiate virulent C. difficile strains that produce active toxins from non-virulent strains that do not produce toxins or produce …

Deciphering The C-Type Lectin Receptor Signaling Pathway In Macrophages In Response To Candida Albicans, Sara Gorjestani

Dissertations and Theses (Open Access)

Candida albicans causes opportunistic fungal infections in humans and is a significant cause of mortality and morbidity in immune-compromised individuals. Dectin-2, a C-type lectin receptor, is required for recognition of C. albicans by innate immune cells and is required for initiation of the anti-fungal immune response. We set out to identify components of the intracellular signaling cascade downstream of Dectin-2 activation in macrophages and to understand their importance in mediating the immune response to C. albicans in vivo. Using macrophages derived from Phospholipase-C-gamma 1 and 2 (PLCγ1and PLCγ2) knockout mice, we demonstrate that PLCγ2, but not PLCγ1, is required …

Yeast Surface Display Of Cd154, Iaryna Masniuk

Graduate Theses and Dissertations

The CD154 (CD40L) is a member of tumor necrosis factor (TNF) family that plays a crucial role in regulation of both cell-mediated and humoral immunity by activating antigen presenting cells (APCs). Previously, studies in the laboratory demonstrated that yeast surface display system allows the expression of biologically functional antigen, such as subunit HA, against influenza virus. Yeast surface display system seems to be an excellent system to be utilized to develop anti-influenza virus vaccine. Thus, it is of interest to study the adjuvant function of CD154 on the subunit HA vaccine against influenza virus. In this project, the CD154 encoding …

The Antimycobacterial Activity Of Hypericum Perforatum Herb And The Effects Of Surfactants, Shujie Shen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tuberculosis is a stubborn and lethal infectious disease. The world-wide tuberculosis surveillance reports that the new TB cases ranges from seven to eight million per year and deaths ranges from 1.3 to 1.6 million every year. The treatment of tuberculosis mainly depends on antituberculosis drugs, such as isoniazid, rifampin, pyrazinamide and ethambutol. However, drug-resistance acquisition is an inevitable process during tuberculosis treatment. Today, multi-drug resistant and the extensively drug-resistant TB cases have been widely reported around the world. Thus, the discovery of novel antituberculosis drugs is essential to effective TB treatment. Hypericum perforatum, also called St. John’s Wort, is …

Compulsory Vaccination Laws: Searching For A Policy-Making Process Immune To Bias, Natalie A. Dana

Seton Hall Journal of Legislation and Public Policy

No abstract provided.

Usf Women's Club Yearbook, 2012–2013, Usf Women's Club

USF Women’s Club Collection

Yearbook of the USF Women's Club. Inside is a list of past presidents, officers, committee chairmen, and members for 2012–2013. The booklet also includes a tribute to Grace Allen, interest groups, calendar of events, and a copy of the constitution and by-laws from the handbook.

Microfluidic Based Human Blood Plasma Separation, Ruizhi Zhong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microfluidic based biotechnologies have been widely applied for biological and biomedical research and applications because the physical scales of microfluidics are suitable for biological cells and provide the ability and flexibility to control spatiotemporal biochemical parameters. The current efforts for biosensor technologies are aiming to develop detection technologies directly from small volumes of the human fluids, such as blood, urea, and saliva, for accurate, fast, and affordable detection and analysis.;Human blood provides information and supports the functioning of human body, such as nourishing tissues, regulating organs activities and defending harmful material. Blood components are the prime interests for biosensor applications …

The Use Of Cortisol And Hba1c As Biomarkers Of Stress In University Administrators, Jacilyn Marie Olson

Graduate Theses and Dissertations

PURPOSE: The purpose of this study was to explore the impact of perceived stress and cardiorespiratory fitness on cortisol and HbA1c, biomarkers of stress, in a group of select university administrators. The impact of gender on these relationships was of special concern. METHODS: University administrators with job titles of Chancellor, Provost, Vice Chancellors and Vice Provosts of the university, and Deans and Associate Deans at the college level were recruited. Twenty-five administrators (15 males and 10 females) agreed to participate and completed a battery of assessments that included completion of the University Administrative Concerns Questionnaire, a finger-stick blood test for …

Hcv Behavioral Health Preparation Of The Racial/Ethnic Minority: Measurement Development Of A Hcv Treatment Readiness Tool In Spanish And English, Michele Vella, Joseph L. Yozviak Do, Facp, Judith Lash Rn

Department of Medicine

No abstract provided.

Inhibition Of Burkholderia Multivorans Adhesion To Lung Epithelial Cells By Bivalent Lactosides, Ciara Wight, Rosaria Leyden, Paul V. Murphy, Máire Callaghan, Trinidad Velasco-Torrijos, Siobhan Mcclean

Articles

Burkholderia cepacia complex (Bcc) is an opportunistic pathogen in cystic fibrosis patients which is inherently resistant to antimicrobial agents. The mechanisms of attachment and pathogenesis of Bcc, a group of 17 species, are poorly understood. The most commonly identified Bcc species in newly colonised patients, Burkholderia multivorans, continues to be acquired from the environment. Development of therapies which can prevent or reduce the risk of colonization on exposure to Bcc in the environment would be a better alternative to antimicrobial agents. Previously, it has been shown that Bcc strains bound to many glycolipid receptors on lung epithelia. Using a …

Refining The Global Spatial Limits Of Dengue Virus Transmission By Evidence-Based Consensus, Oliver J. Brady, Peter W. Gething, Samir Bhatt, Jane P. Messina, John S. Brownstein, Anne G. Hoen

Dartmouth Scholarship

Background: Dengue is a growing problem both in its geographical spread and in its intensity, and yet current global distribution remains highly uncertain. Challenges in diagnosis and diagnostic methods as well as highly variable national health systems mean no single data source can reliably estimate the distribution of this disease. As such, there is a lack of agreement on national dengue status among international health organisations. Here we bring together all available information on dengue occurrence using a novel approach to produce an evidence consensus map of the disease range that highlights nations with an uncertain dengue status.

Methods/Principle Findings: …

Multidrug Resistant Pulmonary Tuberculosis Treatment Regimens And Patient Outcomes: An Individual Patient Data Meta-Analysis Of 9,153 Patients, Shama D. Ahuja, David Ashkin, Monika Avendano, Rita Banerjee, Melissa Bauer, Jamie N. Bayona

Dartmouth Scholarship

Dick Menzies and colleagues report findings from a collaborative, individual patient-level meta-analysis of treatment outcomes among patients with multidrug-resistant tuberculosis.

Background: Treatment of multidrug resistant tuberculosis (MDR-TB) is lengthy, toxic, expensive, and has generally poor outcomes. We undertook an individual patient data meta-analysis to assess the impact on outcomes of the type, number, and duration of drugs used to treat MDR-TB.

Analysis Of Drug Resistance Determinants In Klebsiella Pneumoniae Isolates From A Tertiary-Care Hospital In Beijing, China, Bin Li, Yong Yi, Qi Wang, Patrick C.Y. Woo, Lin Tan, Hua Jing, George F. Gao, Cui Hua Liu

Faculty Publications

Background: The rates of multidrug-resistant (MDR), extensively drug-resistant (XDR) and pandrug-resistant (PDR) isolates among Enterobacteriaceae isolates, particularly Klebsiella pneumoniae, have risen substantially worldwide. Methodology/Principal Findings: To better understand the molecular mechanisms of drug resistance in K. pneumoniae, we analyzed the drug resistance determinants for K. pneumoniae isolates collected from the 306 Hospital, a tertiary-care hospital in Beijing, China, for the period of September 1, 2010-October 31, 2011. Drug susceptibility testing, PCR amplification and sequencing of the drug resistance determinants were performed. Conjugation experiments were conducted to examine the natural ability of drug resistance to disseminate among Enterobacteriaceae strains using a …

July 31, 2012 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Real Time Pcr As A Diagnostic Tool For Hbv Infection In Iraq, Mohammed Ghanim

Iraqi Postgraduate Medical Journal

ABSTRACT : BACKGROUND: HBV infection is a worldwide infectious disease that acutely infects 2 billion yearly, thus finding a precise, accurate and sensitive diagnostic test for this infection is highly advisable. Real Time PCR has been introduced to achieve that mission. OBJECTIVE: Confirm the role of Real Time PCR as a precise tool for diagnosis of HBV infection in Iraq. PATIENTS AND METHODS: In this study, 40 patients of HBV with HBs Antigen positive serological test and 20 individuals with HBs Antigen negative were selected to be the material of the study and they were tested by Real Time PCR …

Inhibitory Effect Of Lactobacilli Filtrate On Klebsiella Pneumoniae Biofilm, Harith J.F. Al-Mathkhury

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: The formation of biofilms by K. pneumoniae is thought to protect the bacteria from antibiotics since these entities are highly resistant to such antimicrobial agents. OBJECTIVE: Investigating the role of Lactobacillus spp. supernatant in eliminating K. pneumoniae biofilms. METHODS: Three hundred and twelve specimens of urine, wounds and sputum were collected from patients attending five hospitals in Baghdad. All specimens were identified using biochemical tests and Api 20 E system. The ability of K. pneumoniae isolates to produce biofilm were evaluated using crystal violet staining technique in pre-sterilized 96-well polystyrene microtiter plates. The effect of MIC of Amikacin …

Inhibitory Effect Of Lactobacilli Filtrate On Klebsiella Pneumoniae Biofilm, Harith J.F. Al-Mathkhury

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: The formation of biofilms by K. pneumoniae is thought to protect the bacteria from antibiotics since these entities are highly resistant to such antimicrobial agents. OBJECTIVE: Investigating the role of Lactobacillus spp. supernatant in eliminating K. pneumoniae biofilms. METHODS: Three hundred and twelve specimens of urine, wounds and sputum were collected from patients attending five hospitals in Baghdad. All specimens were identified using biochemical tests and Api 20 E system. The ability of K. pneumoniae isolates to produce biofilm were evaluated using crystal violet staining technique in pre-sterilized 96-well polystyrene microtiter plates. The effect of MIC of Amikacin …

Comparative Study In Neonates With Septicemia Using Meropenem Versus Ceftriaxone Plus Vancomycin., Huda Y Matloub

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: Neonatal septicemia is an important cause of mortality in both developed and developing countries . The type and pattern of organisms that cause neonatal sepsis changes over time. In addition the causative organisms have developed increased drug resistance for the last two decades . OBJECTIVE: To compare the effectiveness and tolerability of two different antibiotic regimens in the initial treatment of suspected neonatal septicemia. PATIENTS AND METHODS: This is a controlled clinical trial on 80 neonates with a presumptive diagnosis of septicemia who were admitted to the children welfare teaching hospital of Baghdad from August 2010 to March …

Association Of Environmental Traits With The Geographic Ranges Of Ticks (Acari: Ixodidae) Of Medical And Veterinary Importance In The Western Palearctic. A Digital Data Set, A. Estrada-Peña, Robert Farkas, Thomas G.T. Jaenson, Frank Koenen, Maxime Madder, Ilaria Pascucci, Mo Salman, Jordi Tarrés-Call, Frans Jongejan

Harold W. Manter Laboratory of Parasitology: Library Materials

We compiled information on the distribution of ticks in the western Palearctic (11^oW, 45^oE; 29^oN, 71^oN), published during 1970–2010. The literature search was filtered by the tick’s species name and an unambiguous reference to the point of capture. Records from some curated collections were included. We focused on tick species of importance to human and animal health, in particular: Ixodes ricinus, Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. sulcata, Hyalomma marginatum, Hy. lusitanicum, Rhipicephalus annulatus, R. bursa, and the R. sanguineus group. A few records of other species (I. canisuga, I. …