Digital Commons Network^™

Cpg And Interleukin-15 Synergize To Enhance Ifn- Production By Activated Cd8+ T Cells, Dustin Cobb, Siqi Guo, Ronald B. Smeltz

Microbiology and Immunology Publications

Interleukin-15 (IL-15) regulates the development and maintenance of memory CD8+ T cells. Paradoxically, we previously reported that IL-15 could enhance CD8+ T-cell responses to IL-12, a proinflammatory cytokine required for optimal priming of effector CD8+ T cells. To expand the physiological relevance of these findings, we tested IL-15 for its ability to enhance T-cell responses to bacterial CpG. Expectedly, CpG enhanced the production of IFN- by CD8+ T cells polyclonally activated with anti-CD3. However, addition of IL-15 to CpG-stimulated cultures led to a striking increase in IFN- production. The effect of CpG and IL-15 was also evident with CD8+ T …

Treml4, An Ig Superfamily Member, Mediates Presentation Of Several Antigens To T Cells In Vivo, Including Protective Immunity To Her2 Protein, Hiroaki Hemmi, Neeha Zaidi, Bei Wang

Publications

Members of the triggering expressed on myeloid cells (Trem) receptor family fine-tune inflammatory responses. We previously identified one of these receptors, called Treml4, expressed mainly in the spleen, as well as at high levels by CD8α ⁺ dendritic cells and macrophages. Like other Trem family members, Treml4 has an Ig-like extracellular domain and a short cytoplasmic tail that associates with the adaptor DAP12. To follow up on our initial results that Treml4-Fc fusion proteins bind necrotic cells, we generated a knockout mouse to assess the role of Treml4 in the uptake and presentation of dying cells in vivo. Loss of …

Strategy For Identifying Dendritic Cell-Processed Cd4+ T Cell Epitopes From The Hiv Gag P24 Protein, Leonia Bozzacco, Haiqiang Yu, Jörn Dengjel

Publications

Mass Spectrometry (MS) is becoming a preferred method to identify class I and class II peptides presented on major histocompability complexes (MHC) on antigen presenting cells (APC). We describe a combined computational and MS approach to identify exogenous MHC II peptides presented on mouse spleen dendritic cells (DCs). This approach enables rapid, effective screening of a large number of possible peptides by a computer-assisted strategy that utilizes the extraordinary human ability for pattern recognition. To test the efficacy of the approach, a mixture of epitope peptide mimics (mimetopes) from HIV gag p24 sequence were added exogenously to Fms-like tyrosine kinase …

Msum Student Academic Conference, Minnesota State University Moorhead

Student Academic Conference Abstract Books

Minnesota State University Moorhead Student Academic Conference abstract book.

Occupational Health And Safety Management Systems Assessment Training, William Brazile, Daniel Autenrieth, Del Sandfort

Safety Health & Industrial Hygiene

An occupational health and safety management system (OHSMS) is required for all Federal agencies by Executive Order 12196 – Occupational safety and health programs for Federal employees. Development of the Occupational Health and Safety Management Systems Assessment Training Manual was supported jointly by the U.S. Veterans Health Administration (VHA) and the Occupational Safety and Health Administration (OSHA). The manual was designed to be used in the training of government personnel on how to assess the OHSMS of Federal establishments and agencies. The manual is organized sequentially through each step in the process of conducting an OHSMS assessment. Trainees start …

Public Health Impact Of The Hpv Vaccinations: A Research Update, Kathleen Ragan

The Corinthian

In recent years, a large volume of medical and public health literature has been dedicated to the sexually transmitted infection Human Papillomavirus (HPV). The purpose of this paper is to provide a comprehensive assessment of recent empirical investigations which assess the public health impact of HPV and recent HPV vaccinations.

Medical School Watercooler Newsletter - January 1, 2012, Lindsey Lyle

Watercooler Newsletter

This is the January 1, 2012 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• New Poll Demonstrates Importance of Health Research, Development
• January Med School Café - 'Starting Your Year off Right: Making Resolutions a Reality'
• USA College of Medicine Welcomes Dr. Charles Eric McCathran
• Next Week's DSS - Dr. Richard Cone
• Dr. Fortwendel Awarded Research Grant

Toxicological Assessment Of Intra‐Tracheal Instilled Nanoceria On Kidneys Of Sprague‐Dawley Rats, Geeta Nandyala

Theses, Dissertations and Capstones

Recent studies suggest that use of nanoceria in industry is associated with increased risk of human and environmental exposure. How inhaled nanoceria might affect the kidneys is not yet known. To investigate this possibility, Sprague Dawley rats were exposed to a single dose of 7mg/kg body weight cerium oxide nanoparticles by intratracheal instillation. Animals were sacrificed at day 1, 3, 14, or 28 days after exposure and the kidneys collected for histological and biochemical analysis. No significant difference was observed in kidney to body weight ratio between the different groups. Hematoxylin and eosin staining did not reveal any significant changes …

The Effect Of Auditory Call Playback On Anuran Detection And Capture Rates, Derek Adam Bozzell

Theses, Dissertations and Capstones

Calls of male anurans during breeding seasons are species-specific identification tools. However, males cease calling after any nearby disturbance, including those of researchers. I proposed a variation on current methods that attempts to reduce this lag in calling after researcher-created disturbance by utilizing the propensity for competition in male frogs. I surveyed 14 breeding sites in Cabell and Wayne counties during the 2010 and 2011 breeding seasons. First, I used traditional visual encounter surveys (VESs). After using automated recording devices to gather site-specific recordings of calls of all species present, I conducted secondary VESs while playing these playlists over a …

Correlation Of Discharge Diagnostic Codes With Laboratory-Confirmed Methicillin-Resistant Staphylococcus Aureus Bloodstream Infections At A Large Pediatric Hospital, Andrew J. Kreppel

Master of Public Health Program Student Publications

Background: Methicillin-resistant Staphylococcus aureus (MRSA)-specific hospital discharge codes were introduced in October 2008. The purpose of this project is to study the correlation of these codes with laboratory-proven pediatric MRSA bloodstream infections.

Methods: Laboratory and discharge databases were used to identify patientsChildren’s Hospital Medical Center from October 2008 through December 2010 with MRSA bloodstream infections. The laboratory database identified patients with a positive blood culture for MRSA, and the discharge database identified patients with an MRSA bloodstream discharge code (038.12 for MRSA sepsis or 041.12 for MRSA infection with either 790.7 for bacteremia or 771.81 for newborn sepsis or 771.83 …

Redefining Chronic Toxoplasmosis: A T Cell Exhaustion Perspective, Rajarshi Bhadra, Imtiaz A. Khan

Microbiology, Immunology, and Tropical Medicine Faculty Publications

No abstract provided.

The Adjuvanticity Of An O. Volvulus-Derived Rov-Asp-1 Protein In Mice Using Sequential Vaccinations And In Non-Human Primates, Jing Wang, Nancy Trichoche, Lanying Du, Meredith Hunter, Bin Zhan, Gaddam Goud, Elizabeth S. Didier, Jing Liu, Lu Lu, Preston A. Marx, Shibo Jiang, Sara Lustigman

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the Onchocerca volvulusrecombinant protein rOv-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6. However, because it is a protein, the r …

Application Of Nanomedicine In Emergency Medicine: Point-Of-Care Testing And Drug Delivery In Twenty - First Century, Ali Pourmand, Mohammed Reza Pourmand, Justin Wang, Robert Shesser

Emergency Medicine Faculty Publications

The application of emerging nanotechnology to the practice of medicine represents a frontier of nanomedicine. Nanomedicine has been defined as a science which emphasizes the use of nanoscale tools in conjunction with background knowledge of the human body for medical diagnosis and treatment. Application of nanomedicine in EM may give EM providers the opportunity to diagnose and treat life-threatening diseases in a shorter period of time. These applications include diagnostic utilities, preventive medicine, targeted pharmacotherapy, and tissue regeneration.

Impact Of Antiviral Treatment And Hospital Admission Delay On Risk Of Death Associated With 2009 A/H1n1 Pandemic Influenza In Mexico, Gerardo Chowell, Cecile Viboud, Lone Simonsen, Mark A. Miller, Santiago Echevarría-Zuno, Margotx González-León, Víctor H. Borja Aburto

Global Health Faculty Publications

Background

Increasing our understanding of the factors affecting the severity of the 2009 A/H1N1 influenza pandemic in different regions of the world could lead to improved clinical practice and mitigation strategies for future influenza pandemics. Even though a number of studies have shed light into the risk factors associated with severe outcomes of 2009 A/H1N1 influenza infections in different populations (e.g., [1-5]), analyses of the determinants of mortality risk spanning multiple pandemic waves and geographic regions are scarce. Between-country differences in the mortality burden of the 2009 pandemic could be linked to differences in influenza case management, underlying population health, …

Hiv-1 Resistant Cdk2-Knockdown Macrophage-Like Cells Generated From 293t Cell-Derived Human Induced Pluripotent Stem Cells, Marina Jerebtsova, Namita Kumari, Min Xu, Gustavo Brito Alvim De Melo, Xiaomei Niu, Kuan-Teh Jeang, Sergei Nekhai

Pediatrics Faculty Publications

A major challenge in studies of human diseases involving macrophages is low yield and heterogeneity of the primary cells and limited ability of these cells for transfections and genetic manipulations. To address this issue, we developed a simple and efficient three steps method for somatic 293T cells reprogramming into monocytes and macrophage-like cells. First, 293T cells were reprogrammed into induced pluripotent stem cells (iPSCs) through a transfection-mediated expression of two factors, Oct-4 and Sox2, resulting in a high yield of iPSC. Second, the obtained iPSC were differentiated into monocytes using IL-3 and M-CSF treatment. And third, monocytes were differentiated into …

Reasons For Low Pandemic H1n1 2009 Vaccine Acceptance Within A College Sample, Russell D. Ravert, Linda Y. Fu

Pediatrics Faculty Publications

This study examined health beliefs associated with novel influenza A (H1N1) immunization among US college undergraduates during the 2009-2010 pandemic. Undergraduates (ages 18–24 years) from a large Midwestern University were invited to complete an online survey during March, 2010, five months after H1N1 vaccines became available. Survey items measured H1N1 vaccine history and H1N1-related attitudes based on the health belief literature. Logistic regression was used to identify attitudes associated with having received an H1N1 vaccine, and thematic analysis of student comments was conducted to further understand influences on vaccine decisions. Among the 296 students who participated in the survey, 15.2% …

Spread Of White-Nose Syndrome On A Network Regulated By Geography And Climate, Sean P. Maher, Andrew M. Kramer, J. Tomlin Pulliam, Marcus A. Zokan, Sarah E. Bowden, Heather D. Barton, Krisztian Magori, John M. Drake

College of Natural and Applied Sciences

Wildlife and plant diseases can reduce biodiversity, disrupt ecosystem services and threaten human health. Emerging pathogens have displayed a variety of spatial spread patterns due to differences in host ecology, including diffusive spread from an epicentre (West Nile virus), jump dispersal on a network (foot-and-mouth disease), or a combination of these (Sudden oak death). White-nose syndrome is a highly pathogenic infectious disease of bats currently spreading across North America. Understanding how bat ecology influences this spread is crucial to management of infected and vulnerable populations. Here we show that white-nose syndrome spread is not diffusive but rather mediated by patchily …

Call To Action: Responding To New Hampshire's Prescription Drug Abuse Epidemic, The New Hampshire Governor's Commission On Alcohol And Drug Abuse Prevention, Intervention And Treatment

Manchester Research Group

Report of prescription drug abueuse in New Hampshire.

Tetanus Toxoid, Reduced Diphtheria Toxoid And Acellular Pertussis Vaccination And Influenza Vaccination Of Pregnant And Postpartum Women, Colleen M. Mccormick

Master of Public Health Program Student Publications

Introduction: Infants ≤2 months are at greatest risk for morbidity/mortality from pertussis. Tdap vaccines given in late pregnancy or postpartum can protect infants from pertussis. Pregnancy increases risks for maternal and perinatal complications. Influenza vaccine (FLUV) given in pregnancy can protect women and newborns.

Objectives: To determine Tdap and FLUV rates in pregnant and postpartum patients and identify factors associated with vaccination.

Methods: Miami Valley Hospital delivery records from 01/2009-12/2011 were retrospectively reviewed. Data reviewed included age, insurance, race/ethnicity, and county of residence. Descriptive statistics analyzed prevalence of immunization, timing, and demographics. Chi-square and odds ratios detected differences between characteristics. …

Hello Hollywood

Madison Magazine

No abstract provided.

Microbiology Society Taps Jmu Senior

Madison Magazine

The American Society for Microbiology selected Jonathan Barrett ('12) as a 2011 ASM Research Fellow

Community Benefit Report For 2012, Children's Mercy Hospital

Community Benefits

Fiscal year report on the community benefits provided by Children's Mercy Kansas City to the communities in the Kansas City region.

Heeding The Cry For Help: Addressing Lgbt Bullying As A Public Health Issue Through Law And Policy, Christina Meneses, Nicole Grimm

University of Maryland Law Journal of Race, Religion, Gender and Class

No abstract provided.

Virginia Commonwealth University Graduate And Professional Programs Bulletin Courses [2012], Virginia Commonwealth University

VCU Bulletins

Listing of professional and graduate courses for 2012-2013

Minocycline Modulates Antigen-Specific Ctl Activity Through Inactivation Of Mononuclear Phagocytes In Patients With Htlv-I Associated Neurologic Disease, Yoshimi Enose-Akahata, Eiji Matsuura, Yuetsu Tanaka, Unsong Oh, Steven Jacobson

Neurology Publications

Background The activation of mononuclear phagocytes (MPs), including monocytes, macrophages and dendritic cells, contributes to central nervous system inflammation in various neurological diseases. In HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), MPs are reservoirs of HTLV-I, and induce proinflammatory cytokines and excess T cell responses. The virus-infected or activated MPs may play a role in immuneregulation and disease progression in patients with HTLV-I-associated neurological diseases.

Results Phenotypic analysis of CD14+ monocytes in HAM/TSP patients demonstrated high expression of CX3CR1 and HLA-DR in CD14lowCD16+ monocytes, compared to healthy normal donors (NDs) and asymptomatic carriers (ACs), and the production of TNF-α and IL-1β in …

Use Of Controlled Release (Encapsulated Chemical Oxidant) Polymer For Remediation Of Contaminated Water Used For Intentional Biothreat Terrorism And Its Potential Application For Disinfection Of Agricultural Waste Pollution., Christopher Ross Jackson

Theses

The threat of biological agents being used to harm mass populations are of extreme importance in the 21st century. Pathogenic agents such as bacteria, viruses, fungi, protozoan, and helminthes are of major concern in the wake that they can be used to cause death to anyone exposed. Agents like these can be disseminated by air, through water, or from animal, food, or human-to-human transmission. Controlled release methods are extensively used in the medical and agricultural industry to release chemicals at controlled rates for drug delivery in the human body or pesticide treatments for farming. Few researchers have investigated the use …

Evaluating The Effectiveness Of Nurse-Focused Computerized Clinical Decision Support On Urinary Catheter Practice Guidelines, Robin Lynn Neal Lang

Nursing Theses and Capstone Projects

A growing national emphasis has been placed on health information technology (HIT) with robust computerized clinical decision support (CCDS) integration into health care delivery. Catheter-associated urinary tract infection is the most frequent health care-associated infection in the United States and is associated with high cost, high volumes and determined to be preventable through the application of evidence-based guidelines. The purpose of this quasi-experimental, ex post facto study was to evaluate the impact of an evidence-based practice guideline computerized clinical decision support (CCDS) intervention in patients with a urinary catheter device. Correlational relationships were explored among patient and nurse-specific demographics as …

Evidence-Based Litigation Reform, Emily Spottswood

Scholarly Publications

No abstract provided.

Protein-Based Identification Of Quantitative Trait Loci Associated With Malignant Transformation In Two Her2+ Cellular Models Of Breast Cancer, Yogesh M. Kulkarni, David J. Klinke

Faculty & Staff Scholarship

Background

A contemporary view of the cancer genome reveals extensive rearrangement compared to normal cells. Yet how these genetic alterations translate into specific proteomic changes that underpin acquiring the hallmarks of cancer remains unresolved. The objectives of this study were to quantify alterations in protein expression in two HER2+ cellular models of breast cancer and to infer differentially regulated signaling pathways in these models associated with the hallmarks of cancer.

Results

A proteomic workflow was used to identify proteins in two HER2 positive tumorigenic cell lines (BT474 and SKBR3) that were differentially expressed relative to a normal human mammary epithelial …

No. 12: The State Of Food Insecurity In Johannesburg, Michael Rudolph, Florian Kroll, Shaun Ruysenaar, Tebogo Dlamini

African Food Security Urban Network

Johannesburg is the economic hub of South Africa and the Southern African region. At the same time, it is a city of extremes which juxtaposes ostentatious wealth and conspicuous consumption with grinding poverty and food insecurity. Not enough is known about the prevalence and nature of food insecurity in the city, making it dif!cult to challenge and plan to reduce the urban food gap. This paper uses AFSUN data from three lower-income areas of the city (Alexandra, Orange Farm and the Inner City) to examine the characteristics and drivers of food insecurity in Johannesburg. Despite high overall levels of food …