Digital Commons Network^™

Gene Transcription In Sea Otters (Enhydra Lutris); Development Of A Diagnostic Tool For Sea Otter And Ecosystem Health, Lizabeth Bowen, A. Keith Miles, Michael Murray, Martin Haulena, Judy Tuttle, William Van Bonn, Lance Adams, James L. Bodkin, Brenda Ballachey, James A. Estes, M. Tim Tinker, Robin Keister, Jeffrey L. Stott

United States Geological Survey: Staff Publications

Gene transcription analysis for diagnosing or monitoring wildlife health requires the ability to distinguish pathophysiological change from natural variation. Herein, we describe methodology for the development of quantitative real-time polymerase chain reaction (qPCR) assays to measure differential transcript levels of multiple immune function genes in the sea otter (Enhydra lutris); sea otter-specific qPCR primer sequences for the genes of interest are defined. We establish a ‘reference’ range of transcripts for each gene in a group of clinically healthy captive and free-ranging sea otters. The 10 genes of interest represent multiple physiological systems that play a role in immuno-modulation, …

Preliminary Assessment Of The Hoghopper™ For Excluding Non-Target Wildlife, Tyler A. Campbell, Michael J. Bodenchuk, John D. Eisemann, Steven J. Lapidge, Linton Staples, Phil Morrow

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine populations are expanding throughout the U.S., where they are causing increasing amounts of damage to agriculture, natural resources, and property and threaten human health and safety. Methods to control feral swine damage in the U.S. consist of integrated fencing, trapping, snaring, and shooting (including hunting with dogs) efforts. New methods that are being developed to control feral swine damage include toxicants and fertility control agents. For these emerging technologies to be effective at the population level, they must function through oral routes of delivery. Concurrent to the development of orallydelivered actives, a cost-effective system that delivers biologics to …

Effectiveness Of Theobromine And Caffeine Mixtures In Coyote Lure Operative Devices As A Predacide: A Simulated Field Study, Eric M. Gese, Patrick A. Darrow, John A. Shivik, Bruce A. Kimball, John D. Eisemann, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Predators are capable of causing damage to domestic livestock throughout North America. Lethal responses for managing livestock depredations may include the use of sodium cyanide in M- 44 devices. Currently, several states have banned the use of M-44s and several other states are forecast to ban these devices. Therefore, additional tools are being sought to expand the repertoire of options available for managing coyote depredations on domestic livestock. We evaluated the use of a theobromine:caffeine mixture delivered within a Coyote Lure Operative Device (CLOD) as an additional predacide for coyotes (Canis latrans). Results from six trials involving 38 …

Rats! Foiled Again: A History Of Rodent Control Methods Development At The National Wildlife Research Center, Kathleen A. Fagerstone, Michael W. Fall, Gary W. Witmer, William C. Pitt

United States Department of Agriculture Wildlife Services: Staff Publications

The National Wildlife Research Center (NWRC) and its predecessor laboratories have a long history of developing materials and methods for managing rodents and the damage they cause. The NWRC has been influential in exploring, developing, and maintaining legal uses of many traditional field rodenticides such as strychnine and zinc phosphide. Products have been developed for managing rodents in a variety of locales, and for managing a variety of species, from commensal rodents in urban areas, to pocket gophers and mountain beaver in forests, prairie dogs and ground squirrels on rangelands, and nutria and beaver in wetlands. Considerable research has also …

Long-Term Impacts Of Invasive Species On A Native Top Predator In A Large Lake System, Scott A. Rush, Gordon Paterson, Tim B. Johnson, Ken G. Drouillard, Gordon D. Haffner, Craig E. Hebert, Michael T. Arts, Daryl J. Mcgoldric, Sean M. Backus, Brian F. Lantry, Jana R. Lantry, Ted Schaner, Aaron T. Fisk

United States Geological Survey: Staff Publications

1. Declining abundances of forage fish and the introduction and establishment of non-indigenous species have the potential to substantially alter resource and habitat exploitation by top predators in large lakes.

2. We measured stable isotopes of carbon (δ¹³C) and nitrogen (δ¹⁵N) in field-collected and archived samples of Lake Ontario lake trout (Salvelinus namaycush) and five species of prey fish and compared current trophic relationships of this top predator with historical samples.

3. Relationships between δ¹⁵N and lake trout age were temporally consistent throughout Lake Ontario and confirmed the role of lake trout …

Genetic Measures Confirm Familial Relationships And Strengthen Study Design, Stacie J. Robinson, Ryan D. Walrath, Timonthy R. Vandeelen, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Social structure and behavioral interactions between individuals shape basic biological processes, such as breeding; foraging and predator avoidance; movement and dispersal; and disease transmission. We used a targeted trapping strategy to capture kin groups of white-tailed deer (Odocoileus virginianus) during 2007 and 2008 in Sandhill Wildlife Research Area, Wisconsin, USA, in order to observe social behaviors. Because inferring family relationships from observation of behavior is subjective, we usedmeasures of genetic relatedness and parentage assignment tests to determine that our capture strategy was efficient for capturing related pairs (78% of groups contained 1 dyad of related animals). The results of …

Comparative Risk Assessment Of The First-Generation Anticoagulant Rodenticide Diphacinone To Raptors, Barnett A. Rattner, Rebecca S. Lazarus, Karen M. Eisenreich, Katherine E. Horak, Steven F. Volker, Christopher M. Campton, John D. Eisemann, Carol U. Meteyer, John J. Johnston

United States Department of Agriculture Wildlife Services: Staff Publications

New regulatory restrictions have been placed on the use of some second-generation anticoagulant rodenticides in the United States, and in some situations this action may be offset by expanded use of first-generation compounds. We have recently conducted several studies with captive adult American kestrels and eastern screech-owls examining the toxicity of diphacinone (DPN) using both acute oral and short-term dietary exposure regimens. Diphacinone evoked overt signs of intoxication and lethality in these raptors at exposure doses that were 20 to 30 times lower than reported for traditionally used wildlife test species (mallard and northern bobwhite). Sublethal exposure of kestrels and …

Nwrc Chemical Effects Database – What’S Old Is New Again, Jeannette R. O'Hare, John D. Eisemann, Walter A. Bowles Jr., Edward W. Schafer Jr., Kathleen A. Fagerstone

United States Department of Agriculture Wildlife Services: Staff Publications

The United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services (USDA APHIS WS), National Wildlife Research Center (NWRC) “Chemical Effects Database” is an internet-accessible and searchable database that contains bioassay data records for chemicals evaluated for repellency, toxicity, reproductive inhibition, and immobilization of higher vertebrates, and phytotoxicity. These data are of value for environmental risk assessment, conduct of toxicology studies, and the development of safe, effective, and responsible tools to manage vertebrate pest species that cause damage. Chemical screening studies were conducted from 1943 to 1987 by predecessors of the NWRC, and by the U.S. Geological …

Predicting Impacts Of Increased Co2 And Climate Change On The Water Cycle And Water Quality In The Semiarid James River Basin Of The Midwestern Usa, Yiping Wu, Shuguang Liu, Alisa L. Gallant

United States Geological Survey: Staff Publications

Emissions of greenhouse gases and aerosols from human activities continue to alter the climate and likely will have significant impacts on the terrestrial hydrological cycle and water quality, especially in arid and semiarid regions. We applied an improved Soil and Water Assessment Tool (SWAT) to evaluate impacts of increased atmospheric CO₂ concentration and potential climate change on the water cycle and nitrogen loads in the semiarid James River Basin (JRB) in the Midwestern United States. We assessed responses of water yield, soil water content, groundwater recharge, and nitrate nitrogen (NO₃–N) load under hypothetical climate-sensitivity scenarios in terms …

Wildlife Services 2011 Research Needs Assessment, Mark E. Tobin, Stephanie A. Shwiff, John E. Mcconnell, Gary A. Littauer

United States Department of Agriculture Wildlife Services: Staff Publications

The mission of U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services (WS) is to provide federal leadership and expertise in managing problems caused by wildlife. Approximately every 5 years, WS conducts a research needs assessment (RNA) to help to align research priorities at the National Wildlife Research Center (NWRC, the research arm of the WS program) with WS program and customer needs. In 2011, the WS Deputy Administrator solicited input from employees throughout the WS program and representatives from other federal agencies, all 50 state wildlife agencies, various livestock and agricultural commodity groups, and non-governmental organizations. …

Assignment Of Measurable Costs And Benefits To Wildlife Conservation Projects, S. A. Shwiff, A. M. Anderson, R. Cullen, P. C. L. White, S. S. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

Success of wildlife conservation projects is determined bya suite of biological and economic factors. Donorand public understanding of the economic factors is becoming increasingly central to the longevity of funding for conservation efforts. Unlike typical economic evaluation, many costs and benefits related to conservation efforts are realised in nonmonetary tenns. We identify the types of benefits and costs that arise from conservation projects and examine several well developed techniques that economists use to convert benefits and costs into monetary values so they may be compared in a common metric. Costs are typically more readily identifiable than benefits, with financia l …

Deer Mice (Peromyscus Spp.) Biology, Damage And Management: A Review, Gary W. Witmer, Rachael S. Moulton

United States Department of Agriculture Wildlife Services: Staff Publications

The deer mouse is the most widely distributed and abundant small mammal in North America. They use a wide array of habitats, are very adaptable, and have a high reproductive potential. They play a number of roles in ecosystems, but can cause damage to orchards, forests, agriculture crops, and rangelands primarily through seed and newly-emerged seedling consumption. They also cause damage similar to house mice when they occupy buildings or other structures. Deer mice are important components in disease transmission especially of hantaviruses and Lyme disease. Damage reduction methods generally involve lethal control to reduce numbers using rodenticides and traps. …

Introduction: Double-Crested Cormorants Of The Great Lakes – St. Lawrence River Basin: Recent Studies, Movements And Responses To Management Actions, D.V. Chip Weseloh, Francesca J. Cuthbert, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

This paper introduces the second Special Publication of the Waterbird Society to address the biology and management of the Double-crested Cormorant (Phalacrocorax auritius) in North America. Since the late 1960s and early 1970s, when the species was at very low population levels, the Double-crested Cormorant has rebounded to its greatest population level in over 100 years. Such a significant increase has resulted in changes in community structure, and new stressors, in many aquatic ecosystems. Both Special Publications (1995 and 2013) have been focused on the biology and management of the species. The first volume dealt mainly with population …

Corrigendum To “Avian Use Of Solid Waste Transfer Stations” [Landscape Urban Plan. 104 (2012) 388–394], Brian E. Washburn

United States Department of Agriculture Wildlife Services: Staff Publications

No abstract provided.

Efficacy Of Proximity Loggers For Detection Of Contacts Between Maternal Pairs Of White-Tailed Deer, Ryan Walrath, Timonthy R. Vandeelen, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Contact frequency and duration estimates between individuals are important to understanding the behavioral ecology of wildlife species and the epidemiology of infectious diseases. A new technology uses proximity data loggers to record time and duration of contacts. We conducted an experiment at Sandhill Wildlife Management Area, located near Babcock, Wisconsin (USA) to compare probabilities of detecting intraspecific contacts among white-tailed deer (Odocoileus virginianus) maternal pairs (dams/fawns) based on detections from proximity loggers deployed on collars versus those obtained from direct observation. We defined 5 discrete probabilities of detection of a contact in terms of P (probability of detection …

Safety And Immunogenicity Of Onrab® In Raccoons And Skunks In West Virginia – 2011 Field Trial Report, D. D. Slate, R. Chipman, K. Nelson, C. Croson, S. Mills, C. Rupprecht, K. C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In 2011, a field trial was conducted in southeastern West Virginia to evaluate the safety and immunogenicity of a live recombinant human adenovirus (serotype 5)-rabies glycoprotein vaccine (ONRAB®) in wild raccoons and skunks. ONRAB® was selected for evaluation based on the success achieved in eliminating raccoon rabies from Ontario and Quebec, Canada, through the integration of this vaccine into rabies management strategies and significantly higher sero-positivity in comparison to Raboral V-RG® in studies along the U.S.-Canada border. The field trial site was composed of forested and agricultural habitats in an area where raccoon rabies is enzootic and oral rabies vaccine …

Invasives: The High Cost Of Indifference, Mark Pfost, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

At one time, wildlife professionals may have been blissfully ignorant about invasive species, classifying “plants” as lawn grass, flowers, trees, or weeds, and putting animals into two groups: species with bag limits and everything else. But those days of ignorance are long past. We see how invasives threaten local, even global, ecosystems, and we need to help create greater awareness about the scope of the problem.

Wildlife professionals must not only prepare to deal with how seven billion people, climate change, and habitat loss will affect native species and habitats, but also how those species and habitats will be affected …

Gonadal Abnormalities In Frogs (Lithobates Spp.) Collected From Managed Wetlands In An Agricultural Region Of Nebraska, Usa, Diana M. Papoulias, Matt S. Schwarz, Lourdes Mena

United States Geological Survey: Staff Publications

Nebraska’s Rainwater Basin (RWB) provides important wetland habitat for North American migratory birds. Concern exists that pesticide and nutrient runoff from surrounding row-crops enters wetlands degrading water quality and adversely affecting birds and wildlife. Frogs may be especially vulnerable. Plains leopard (Lithobates blairi) metamorphs from RWB wetlands with varying concentrations of pesticides were evaluated for a suite of biomarkers of exposure to endocrine active chemicals. Froglets had ovarian dysgenesis, high rates of testicular oocytes, and female-biased sex ratios however, there was no clear statistical association between pesticide concentrations and biomarkers. Data interpretation was hindered because timing and duration …

History And Evaluation Of National-Scale Geochemical Data Sets For The United States, David B. Smith, Steven M. Smith, John D. Horton

United States Geological Survey: Staff Publications

Six national-scale, or near national-scale, geochemical data sets for soils or stream sediments exist for the United States. The earliest of these, here termed the ‘Shacklette’ data set, was generated by a U.S. Geological Survey (USGS) project conducted from 1961 to 1975. This project used soil collected from a depth of about 20 cm as the sampling medium at 1323 sites throughout the conterminous U.S. The National Uranium Resource Evaluation Hydrogeochemical and Stream Sediment Reconnaissance (NUREHSSR) Program of the U.S. Department of Energy was conducted from 1975 to 1984 and collected either stream sediments, lake sediments, or soils at more …

Remote Sensing Of Evapotranspiration For Operational Drought Monitoring Using Principles Of Water And Energy Balance, Gabriel B. Senay, Stefanie Bohms, James P. Verdin

United States Geological Survey: Staff Publications

Evapotranspiration (ET) is an important component of the hydrologic budget because it reflects the exchange of mass and energy between the soil–water–vegetation system and the atmosphere. Prevailing weather conditions influence potential or reference ET through variables such as radiation, temperature, wind, and relativity humidity. In addition to these weather variables, actual ET (ET_a) is also affected by land cover type and condition, as well as soil moisture. The dependence of ET_a on land cover and soil moisture, and its direct relationship with carbon dioxide assimilation in plants, makes it an important variable for monitoring drought, crop yield, …

Boots On The Ground: A First-Hand Account Of Conducting Psychological Research In Combat, Peter D. Harms, Paul B. Lester

P. D. Harms Publications

At the height of the surge in Iraq, Captain Paul Lester, an Army research psychologist, embedded himself with the 2nd Battalion, 6th Infantry Regiment in order to study leadership and psychological resilience in combat. Few researchers are ever presented with the opportunities and responsibilities inherent for a psychologist in this situation or with the decisions he faced when agreeing to take on this mission. In his own words, Captain Lester explains the nature of this mission, what makes research in this context so unique, and what lessons he learned.

Hepatic Abc Transporters And Triglyceride Metabolism, John S. Parks, Soonkyu Chung, Gregory S. Shelness

Department of Nutrition and Health Sciences: Faculty Publications

Purpose of review—Elevated plasma triglyceride and reduced HDL concentrations are prominent features of metabolic syndrome and type 2 diabetes. Individuals with Tangier disease also have elevated plasma triglyceride concentrations and very low HDL, resulting from mutations in ATP-binding cassette transporter A1 (ABCA1), an integral membrane protein that facilitates nascent HDL particle assembly. Past studies attributed the inverse relationship between plasma HDL and triglyceride to intravascular lipid exchange and catabolic events. However, recent studies also suggest that hepatic signaling and lipid mobilization and secretion may explain how HDL affects plasma triglyceride concentrations.

Recent findings—Hepatocyte-specific ABCA1 knockout mice have markedly reduced plasma …

Successful Aging: A Psychosocial Resources Model For Very Old Adults, G. Kevin Randall, Peter Martin, Mary Ann Johnson, Leonard W. Poon

Department of Nutrition and Health Sciences: Faculty Publications

Objectives.Using data from the first two phases of the Georgia Centenarian Study, we proposed a latent factor structure for the Duke OARS domains: Economic Resources, Mental Health, Activities of Daily Living, Physical Health, and Social Resources. Methods. Exploratory and confirmatory factor analyses were conducted on two waves of the Georgia Centenarian Study to test a latent variable measurement model of the five resources; nested model testing was employed to assess the final measurement model for equivalency of factor structure over time. Results. The specified measurement model fit the data well at Time 1. However, at Time 2, …

Regulation Of P53 Stability And Apoptosis By A Ror Agonist, Yongjun Wang, Laura A. Solt, Douglas J. Kojetin, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

Activation of p53 function leading to cell-cycle arrest and/or apoptosis is a promising strategy for development of anticancer therapeutic agents. Here, we describe a novel mechanism for stabilization of p53 protein expression via activation of the orphan nuclear receptor, RORa. We demonstrate that treatment of cancer cells with a newly described synthetic ROR agonist, SR1078, leads to p53 stabilization and induction of apoptosis. These data suggest that synthetic ROR agonists may hold utility in the treatment of cancer.

Regulation Of Expression Of Citrate Synthase By The Retinoic Acid Receptor-Related Orphan Receptor A (Rorα), Christine Crumbley, Yongjun Wang, Subhashis Banerjee, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

The retinoic acid receptor-related orphan receptor α (RORα) is a member of the nuclear receptor superfamily of transcription factors that plays an important role in regulation of the circadian rhythm and metabolism. Mice lacking a functional RORα display a range of metabolic abnormalities including decreased serum cholesterol and plasma triglycerides. Citrate synthase (CS) is a key enzyme of the citric acid cycle that provides energy for cellular function. Additionally, CS plays a critical role in providing citrate derived acetyl-CoA for lipogenesis and cholesterologenesis. Here, we identified a functional RORα response element (RORE) in the promoter of the CS gene. ChIP …

Spatially Explicit Models For Inference About Density In Unmarked Or Partially Marked Populations, Richard B. Chandler, J. Andrew Royle

United States Geological Survey: Staff Publications

Recently developed spatial capture-recapture (SCR) models represent a major advance over traditional capture-recapture (CR) models because they yield explicit estimates of animal density instead of population size within an unknown area. Futhermore, unlike nonspatial CR methods, SCR models account for heterogeneity in capture probability arising from the juxtaposition of animal activity centers and sample locations. Although the utility of SCR methods is gaining recognition, the requirement that all individuals can be uniquely identified exludes their use in many contexts. In this paper, we develop models for situations in which individual recognition is not possible, thereby allowing SCR concepts to be …

The Impact Of University Patenting On Mobility Of Scientists, Xi Ye

Dissertations and Theses Collection (Open Access)

After the passage of Bayh-Dole Act, universities have been actively involved in patenting. At the same time, the booming of university patenting has brought up huge controversies and debates in academia. A large body of literature is devoted, from a broad macro-level view, to investigate the impact of intellectual property rights (IPR) on the research activities of universities. However, very few empirical studies have been conducted to study the impact of university patenting on the mobility of individuals who have been granted these patents. This study, aiming to provide a different insight to the extant literature, employs data from U.S. …

The Impact Of Perceived Csr On Employee Performance And Turnover Intention: An Examination Of The Mediating Effect Of Organizational Justice And Organization-Based Self-Esteem, Alicia Ting Shiun Ho

Dissertations and Theses Collection (Open Access)

Research on corporate social responsibility (CSR) has spanned across a few decades and in various fields. Yet only a handful of academic studies have investigated the relationship between CSR and a commonly neglected stakeholder – the employees. The employees are an essential part of the organization and will be highly influenced by the CSR initiatives carried out by the organization. In my paper, I intend to bridge the gap between CSR and the employee. A theoretical model is presented to show how employees' perception of CSR subsequently impacts their performance and turnover intentions, mediated by organizational justice and organization-based self-esteem. …

Resistance Patterns Of Mycobacterium Tuberculosis Isolates From Pulmonary Tuberculosis Patients In Nairobi, Perpetual Wangui Ndung’U, Samuel Kariuki, Zipporah Ng’Ang’A, Gunturu Revathi

Pathology, East Africa

Introduction: In Kenya, which ranks thirteenth of 27 high tuberculosis burden countries, diagnosis is based on Ziehl-Neelsen staining alone and patients are treated without information on sensitivity patterns. This study aimed to determine resistance patterns of Mycobacterium tuberculosis isolated from pulmonary samples.

Methodology: Pulmonary tuberculosis patients in Nairobi were randomly sampled after informed consent and recruited into the study using a structured questionnaire. Specimens were cultured in liquid and solid media, and drug susceptibility tests were performed for first-line drugs including (isoniazid, rifampin, streptomycin, ethambutol and pyrazinamide).

Results: Eighty-six (30%) of 286 isolates were resistant to at least one of …

Child Representation In America: Progress Report From The National Quality Improvement Center, Donald N. Duquette, Julian Darwall

Articles

Few dispute that children in the child welfare system need effective representation. In October 2009, the U.S. Children's Bureau named the University of Michigan Law School the National Quality Improvement Center on the Representation of Children in the Child Welfare System (QIC-ChildRep). The QIC-ChildRep is a five-year, multimillion dollar project, charged with gathering, developing, and communicating knowledge on child representation. In addition, the QIC-ChildRep is tasked with promoting a consensus on the role of the child's legal representative and providing one of the first random assignment experimental design research projects on the legal representation of children.