Digital Commons Network^™

Separation Of Alcohol Soluble Sorghum Proteins Using Non-Porous Cation-Exchange Columns, Deidre L. Blackwell, Scott R. Bean

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Kafirins, the storage proteins and major protein of the cereal grain sorghum, play an important nutritional role for millions of people in parts of Africa and Asia. Kafirins are non-water soluble, being soluble only in the presence of detergents or aqueous alcohol mixtures and are among the most hydrophobic of the cereal proteins. Limited M_w heterogeneity of kafirins reduces their resolution when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Charge based separation techniques have been shown to have improved resolution of kafirins, but due to the nature of their solubility, ion-exchange (IE)-HPLC has not been widely used to …

Spillover Of Functionally Important Organisms Between Managed And Natural Habitats, Eleanor J. Blitzer, Carsten F. Dormann, Andrea Holzschuh, Alexandra-Maria Klein, Tatyana A. Rand, Teja Tscharntke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Land-use intensification has led to a landscape mosaic that juxtaposes human-managed and natural areas. In such human-dominated and heterogeneous landscapes, spillover across habitat types, especially in systems that differ in resource availability, may be an important ecological process structuring communities. While there is much evidence for spillover from natural habitats to managed areas, little attention has been given to flow in the opposite direction. This paper synthesizes studies published to date from five functionally important trophic groups, herbivores, pathogens, pollinators, predators, and seed dispersers, and discusses evidence for spillover from managed to natural systems in all five groups. For each …

Evidence For A Major Qtl Associated With Host Response To Porcine Reproductive And Respiratory Syndrome Virus Challenge, N. Boddicker, E. H. Waide, R. R. R. Rowland, J. K. Lunney, D. J. Garrick, J. M. Reecy, J. C. M. Dekkers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) causes decreased reproductive performance in breeding animals and increased respiratory problems in growing animals, which result in significant economic losses in the swine industry. Vaccination has generally not been effective in the prevention of PRRS, partially because of the rapid mutation rate and evolution of the virus. The objective of the current study was to discover the genetic basis of host resistance or susceptibility to the PRRS virus through a genome-wide association study using data from the PRRS Host Genetics Consortium PRRS-CAP project.

Three groups of approximately 190 commercial crossbred pigs from 1 breeding …

Influence Of Environmental Temperature On The Physiological, Endocrine, And Immune Responses In Livestock Exposed To A Provocative Immune Challenge, J.A. Carroll, N.C. Burdick, C. C. Chase Jr, S. W. Coleman, D. E. Spiers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Although livestock experience many stressors throughout their life, one of the most commonly experienced, and most difficult to control, is stress caused by fluctuations in environmental temperatures that extend beyond the thermoneutral (TN) zone for an animal. In swine, cold stress has long been recognized as a main cause of neonatal morbidity and mortality. A possible explanation for this increased morbidity and mortality may be related to their inability to generate a febrile response. Previously, we reported that the acute phase immune response, including the generation of fever, after exposure to lipopolysaccharide (LPS; Escherichia coli O111: B4; Sigma-Aldrich, St Louis, …

Animal Foraging As A Mechanism For Sediment Movement And Soil Nutrient Development: Evidence From The Semi-Arid Australian Woodlands And The Chihuahuan Desert, David J. Eldridge, Terry B. Koen, Aaron Killgore, Niki Huang, Walter G. Whitford

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An emerging area of interest in geomorphology over the past two decades has been the effects of biota on ecosystemprocesses.We examinedthe roles of a rangeof vertebrates onsoildisturbance in twomarkedlydifferent environments, the semi-arid woodland of eastern Australia and a Chihuahuan Desert grassland–shrubland in the south-westernUnited States. Foraging pits of soil-disturbingvertebrates variedmarkedly fromsmall scratchings of heteromyid (mainly Dipodomys spp.) rodents (1.8×10⁻⁴m³) to deep (1.0×10⁻² m³) excavations of the burrowing bettong (Bettongia leuseur) and greater bilby (Macrotis lagotis). Vertebratesmoved substantial volumes of soil in both environments, and activity was highly temporally and …

Genomics Of Tropical Fruit Tree Crops, R. S. Arias, James W. Borrone, Cecile L. Tondo, David N. Kuhn, Brian M. Irish, Raymond J. Schnell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The genetic improvement of tropical fruit trees is limited when compared to progress achieved in temperate fruit trees and annual crops. Tropical fruit tree breeding programs require significant resources to develop new cultivars that are adapted to modern shipping and storage requirements. The use of molecular markers in tropical fruit tree breeding is greatly assisting in solving a number of difficult challenges for breeders such as the development of complex family structures for recombination mapping and for recurrent selection. A review of the literature on molecular markers development and new techniques for increasing single-nucleotide polymorphic markers is discussed. The development …

Registration Of ‘Denali’ Wheat, Scott D. Haley, Jerry J. Johnson, Frank B. Peairs, John A. Stromberger, Emily E. Hudson, Scott A. Seifert, Rebecca A. Kottke, Victoria A. Valdez, Jeff B. Rudolph, Terry J. Martin, Guihua Bai, Xianming Chen, Robert L. Bowden, Yue Jin, James A. Kolmer, Ming-Shun Chen, Bradford W. Seabourn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

‘Denali’ (Reg. No. CV-1075, PI 664256) hard red winter wheat (Triticum aestivum L.) was developed by the Colorado Agricultural Experiment Station and released cooperatively by Colorado State University (CSU) and Kansas State University (KSU) in August 2011 through a marketing agreement with the Colorado Wheat Research Foundation. In addition to researchers at CSU and KSU, USDA-ARS researchers at Manhattan, KS, St. Paul, MN, and Pullman, WA participated in its development. Denali was selected from the cross CO980829/‘TAM 111’ made in 2001 at Fort Collins, CO. CO980829 is an experimental line from CSU with the pedigree ‘Yuma’ (PI 559720)/PI 372129//CO850034/3/4*Yuma/4/NEWS12. …

Reduction Of Mosquito Biting Pressure By Timed-Release 0.3% Aerosolized Geraniol, Edita E. Revay, Amy Junnila, Daniel L. Kline, Rui-De Xue, Ulrich R. Bernier, Vasiliy D. Kravchenko, Zoya Yefremova, Günter Müller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We conducted a study to determine the degree of personal protection provided by the Terminix^® ALLCLEAR^® Mosquito Mister – Lantern Edition. This outdoor unit was operated to disperse an aerosolized aqueous 0.3% geraniol emulsion in timed-release intervals of 5.0, 7.5, and 10.0 min. Human volunteers participated in landing catch experiments to test the effect of geraniol sprayed at pre-set time intervals, at two distances: (1) 18 ft (5.49 m), the maximum effective distance claimed by the manufacturer, and (2) 9 ft (2.74 m), half the effective distance from the unit. When aerosolized geraniol was dispensed, reductions in biting …

Natural Product Studies Of U.S. Endangered Plants: Volatile Components Of Lindera Melissifolia (Lauraceae) Repel Mosquitoes And Ticks, Joonseok Oh, John J. Bowling, John F. Carroll, Betul Demirci, K. Hüsnü Can Baser, Theodor D. Leininger, Ulrich R. Bernier, Mark T. Hamann

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The number of endangered plant species in the U.S. is significant, yet studies aimed towards utilizing these plants are limited. Ticks and mosquitoes are vectors of significant pathogenic diseases of humans. Repellents are critical means of personal protection against biting arthropods and disease transmission. The essential oil and solvent extracts from Lindera melissifolia (Walt.) Blume (Lauraceae) (pondberry) drupes were gathered and analyzed by GC and GC–MS. The essential oil obtained from this endangered plant showed a significant dose dependent repellency of ticks and a moderate mosquito repellent effect while the subsequent hexanes extract was completely ineffective. Fractional freezing enriched the …

Indirect Effects Of Parasites In Invasions, Alison M. Dunn, Mark E. Torchin, Melanie J. Hatcher, Peter M. Kotanen, Dana M. Blumenthal, James E. Byers, Courtney A.C. Coon, Victor M. Frankel, Robert D. Holt, Ruth A. Hufbauer, Andrew R. Kanarek, Kristina A. Schierenbeck, Lorne M. Wolfe, Sarah E. Perkins

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Introduced species disrupt native communities and biodiversity worldwide. Parasitic infections (and at times, their absence) are thought to be a key component in the success and impact of biological invasions by plants and animals. They can facilitate or limit invasions, and positively or negatively impact native species.

2. Parasites have not only direct effects on their hosts, but also indirect effects on the species with which their hosts interact. Indirect effects include density-mediated effects (resulting from parasite-induced reduction in host reproduction and survival) as well as trait-mediated indirect effects (resulting from parasite-induced changes in host phenotype, behavior or life …

Compositional And Mechanical Properties Of Peanuts Roasted To Equivalent Colors Using Different Time/Temperature Combinations, Kristin A. Mcdaniel, Brittany L. White, Lisa L. Dean, Timothy H. Sanders, Jack P. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanuts in North America and Europe are primarily consumed after dry roasting. Standard industry practice is to roast peanuts to a specific surface color (Hunter L-value) for a given application; however, equivalent surface colors can be attained using different roast temperature/time combinations, which could affect product quality. To investigate this potential, runner peanuts from a single lot were systematically roasted using 5 roast temperatures (147, 157, 167, 177, and 187 ^◦C) and to Hunter L-values of 53 ± 1, 48.5 ± 1, and 43 ± 1, corresponding to light, medium, and dark roasts, respectively. Moisture contents (MC) …

Persistence Of Mycobacterium Bovis Bacillus Calmette– Guerin (Bcg) Danish In White-Tailed Deer (Odocoileus Virginianus) Vaccinated With A Lipid-Formulated Oral Vaccine, M. V. Palmer, T. C. Thacker, W. R. Waters, S. Robbe-Austerman, F. E. Aldwell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacterium bovis, the causative agent of tuberculosis in animals, has a broad host range, including humans. Historically, public health concerns prompted programs to eradicate tuberculosis from cattle in many nations. Eradication efforts decreased the prevalence of bovine tuberculosis; nevertheless, some countries encountered significant obstacles, not least of which was a wildlife reservoir of M. bovis. Efforts to decrease the size of the affected wildlife populations have neither eliminated disease nor eliminated transmission to cattle. Consequently, the use of a vaccine for wildlife is being explored. The vaccine most studied is M. bovis BCG, an attenuated live vaccine, first …

Susceptibility Of Three Strains Of Blue Catfish, Ictalurus Furcatus (Valenciennes), To Ichthyophthirius Multifiliis, D-H Xu, P.H. Klesius, Brian G. Bosworth, N Chatakondi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study compared the susceptibility of three blue catfish strains (D&B, USDA 101 and USDA 102) to the parasite Ichthyophthirius multifiliis (Ich). In Trial I, a cohabitation study (all strains stocked communally) was conducted and fish were exposed to theronts at 0, 200, 1000, 5000 or 25 000 theronts fish^-1, respectively. All fish died when exposed to theronts at 5000 or 25 000 theronts fish^-1. When exposed to 1000 theronts fish^-1, USDA 102 strain of blue catfish showed significantly lower mortality (78.5%) compared to USDA 101 and D&B strains (92.7% and 100%). In Trial …

Antibacterial Activity Of Cinnamaldehyde And Sporan Against Escherichia Coli O157:H7 And Salmonella, Nadine Yossa, Jitendra Patel, Dumitru Macarisin, Patricia Millner, Charles Murph, Gary Bauchan, Y. Martin Lo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The in vitro antimicrobial effect of cinnamaldehyde and Sporan in combination with acetic acid against Escherichia coli O157:H7 and Salmonella was investigated in Luria-Bertani broth (7 log cfu/mL) containing cinnamaldehyde or Sporan (800 and 1,000 ppm) alone or in combination with 200 ppm acetic acid, and incubated at 37C for up to 6 h. Surviving populations of test pathogens were determined by spiral plating on selective media. E. coli O157:H7 and Salmonella were undetectable after 1 h in the presence of 800 ppm cinnamaldehyde. A 1,000 ppm Sporan significantly reduced Salmonella and E. coli O157:H7 populations by 1.83 and 3.02 …

Soil Maps Of Wisconsin, Alfred E. Hartemink, Birl Lowery, Carl Wacker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Legacy soil maps are an important input in digital soil mapping. This paper traces how reconnaissance soil maps in Wisconsin evolved between the 1880s and the present with some discussion on future directions. The first soil map in the USA was made in Wisconsin by the geologist T.C. Chamberlin in 1882. The second soil map of Wisconsin was made by A.R. Whitson in 1927, and the third by F.D. Hole in 1976. Soil texture and physiography were the major diagnostic mapping criteria. As more detailed county soil surveys were completed and knowledge of the soils increased, a higher level of …

Early Weaning In Northern Great Plains Beef Cattle Production Systems: Iii. Steer Weaning, Finishing And Carcass Characteristics, R.C. Waterman, T.W. Geary, J. A. Paterson, R.J. Lipsey, W.R. Shafer, L. L. Berger, D. B. Faulkner, J.W. Homm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Studies were conducted to evaluate the effect of early weaning steer calves on BW gain, feedlot performance, and carcass characteristics in two herds located in the Northern Great Plains, USA. Steer calves from predominantly Angus x Hereford dams were stratified within damage and calving date (Fort Keogh Livestock and Range Research Laboratory (LARRL), Miles City, MT, USA; n=354)and randomly assigned to one of three weaning treatments. In addition, steer calves from Angus and Angus x Simmental dams (n=200; Judith Gap (JG), MT, USA) were stratified within breed group by age, calving date, and AI sire. Steer calves …

Chapter 2 Switchgrass Breeding, Genetics, And Genomics, Michael D. Casler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass was one of the dominant species of the North American tallgrass prairie and savanna ecosystems that once dominated a large portion of the continent. It is currently used for pasture, hay production, soil conservation, and biomass production for conversion to energy. Switchgrass was selected in 1992 as the herbaceous model species to develop dedicated cellulosic bioenergy crops. Breeding and genetics studies began on switchgrass in the 1950s, focused on utilization in livestock agriculture. Recent developments have rapidly increased the rate of gain for biomass yield, largely by increasing the focus and intensity of selection and improving the choice of …

Prevalence And Genotypes Of Enterocytozoon Bieneusi In Weaned Beef Calves On Cow-Calf Operations In The Usa, Mónica Santín, David Dargatz, Ronald Fayer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To determine the prevalence and genotype distribution of Enterocytozoon bieneusi in weaned beef calves in the USA, fecal samples were collected from 819 calves (6– 18 months of age) from 49 operations. Feces were sieved and subjected to density gradient centrifugation to remove fecal debris and to concentrate spores. DNA extracted from each sample was subjected to the polymerase chain reaction (PCR) to amplify the complete internal transcriber spacer (ITS). All PCR-positive specimens were sequenced to determine the genotype(s) present. Overall, E. bieneusi was detected in 34.8% of the 819 fecal samples. The highest prevalence was found in the Midwest …

Elevated Co2 Affects Plant Responses To Variation In Boron Availability, Sasmita Mishra, Scott A. Heckathorn, Jonathan M. Frantz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aim Effects of elevated CO₂ on N relations are well studied, but effects on other nutrients, especially micronutrients, are not. We investigated effects of elevated CO₂ on response to variation in boron (B) availability in three unrelated species: seed geranium (Pelargonium x hortorum), barley (Hordeum vulgare), and water fern (Azolla caroliniana).

Methods Plants were grown at two levels of CO₂ (370, 700 ppm) and low, medium, and high B. Treatment effects were measured on biomass, net photosynthesis (P_n) and related variables, tissue nutrient concentrations, and B transporter protein BOR1. …

Impaired Object Tracking In Children With Chromosome 22q11.2 Deletion Syndrome, Margarita H. Cabaral, Elliott A. Beaton, Joel Stoddard, Tony J. Simon

Psychology Faculty Publications

Background:

Chromosome 22q11.2 Deletion Syndrome (22q11.2DS) occurs in approximately 1:4,000 live births with a complex and variable presentation that includes medical, socioemotional and psychological symptoms with intellectual impairment. Cognitive impairments in spatiotemporal and visuospatial attention have also been reported. However, maintenance of selective attention to dynamic and interacting objects has not been systematically investigated in children with 22q11.2DS.

Methods:

We used a multiple object tracking task to assay capacity and resolution performance of children with 22q11.2DS aged 7 to 14 years versus age-matched typically developing (TD) peers.

Results:

Children with 22q11.2DS but not TD children demonstrated impaired performance when task …

The Effects Of Summer Camp On Self-Esteem And Self-Concept In Children Diagnosed With Cancer, Meghan E. Connell

All NMU Master's Theses

No abstract provided.

A Molecular Genetic Assessment Of Sex Ratios From Breeding, Migratory And Overwintering Common Loons, Abigail Leigh Debiak

All NMU Master's Theses

No abstract provided.

Do Not Resuscitate And Other Imperatives, Stacy B. Harrison

All NMU Master's Theses

No abstract provided.

In Mirrors And Through Windows, Laura Mead

All NMU Master's Theses

No abstract provided.

Classification Of Mental Illness In The 18th Century: A Comparison Of The Nosologies Of Carl Linnaeus, Francois Bossier De Sauvages, Rudolpho Vogel And William Cullen, Heather Ann Munsche

All NMU Master's Theses

No abstract provided.

The Use Of Single And Paired Suppressive Strains Of Streptomyces Species To Reduce Soilborne Pathogenic Populations Of Streptomyces Scabies And Reduce Symptoms And Occurrence Of Potato Scab Disease, Michael Jay Peters

All NMU Master's Theses

No abstract provided.

Effects Of 1Α,25-Dihydroxyvitamin D3 (Calcitriol) On Cultured Glioblastoma Multiforme Cells, Justine M. Pinskey

All NMU Master's Theses

No abstract provided.

Michigan Prisoner Reentry Initiative And Sex Offenders: An Exploratory Study Of A Sex Offender Transition Program, Lynda Rayburn-James

All NMU Master's Theses

No abstract provided.

The Brontёs And The Beasts: Newly Awakened Sympathies And Shifting Representations Of Other-Than-Human Animals In Victorian Literature, Teresa Sherman-Jones

All NMU Master's Theses

No abstract provided.

Inhibition Of Mir26a Microrna Restores Sensitivity To Temozolomide, Angela G. Stewart

All NMU Master's Theses

No abstract provided.