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Regulation Of Metabolic Stress-Induced Snornas, Benjamin Steel Scruggs

All Theses and Dissertations (ETDs)

Accumulation of excess lipid in non-adipose tissues is associated with oxidative stress and organ dysfunction and plays an important role in diabetic complications. While a number of stress responses have been implicated, the precise molecular mechanisms linking lipid accumulation and cellular dysfunction are not fully understood. To elucidate molecular events critical for lipotoxicity, we used retroviral promoter trap mutagenesis to generate mutant Chinese hamster ovary cell lines resistant to lipotoxic and oxidative stress. This approach uncovered a previously unsuspected role for small nucleolar RNAs: snoRNAs) as critical mediators of lipotoxic cell death.

Herein we show that under lipotoxic conditions, intronic …

Molecular Characterization And Tissue-Specific Gene Expression Of Dermacentor Variabilis Α-Catenin In Response To Rickettsial Infection, Piyanate Sunyakumthorn, Natthida Petchampai, Michael T. Kearney, Daniel E. Sonenshine, Kevin R. Macaluso

Biological Sciences Faculty Publications

Alpha catenin is a cytoskeleton protein that acts as a regulator of actin rearrangement by forming an E-cadherin adhesion complex. In Dermacentor variabilis, a putative α-catenin (Dvα-catenin) was previously identified as differentially regulated in ovaries of ticks chronically infected with Rickettsia montanensis. To begin characterizing the role(s) of Dvα-catenin during rickettsial infection, the full-length Dvα-catenin cDNA was cloned and analysed. Comparative sequence analysis demonstrates a 3069-bp cDNA with a 2718-bp open reading frame with a sequence similar to Ixodes scapularis α-catenin. A portion of Dvα-catenin is homologous to the vinculin-conserved domain containing a putative …

Protection By 20-5,14-Hedge Against Surgically-Induced Ischemia Reperfusion Lung Injury In Rats, Irshad Ali, Stephanie Gruenloh, Ying Gao, Anne V. Clough, John R. Falck, Meetha Medhora, Elizabeth R. Jacobs

Mathematics, Statistics and Computer Science Faculty Research and Publications

Background

We previously reported that the cytochrome P450 product 20-hydroxyeicosatetraenoic acid has prosurvival effects in pulmonary artery endothelial cells and ex vivo pulmonary arteries. We tested the potential of a 20-hydroxyeicosatetraenoic acid analog N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine (20-5,14-HEDGE) to protect against lung ischemic reperfusion injury in rats. Furthermore, we examined activation of innate immune system components, high mobility group box 1 (HMGB1) and toll-like receptor 4 (TLR4), in this model as well as the effect of 20-5,14-HEDGE on this signaling pathway.

Methods

Sprague-Dawley rats treated with 20-5,14-HEDGE or vehicle were subjected to surgically induced, unilateral lung ischemia for 60 minutes followed by reperfusion …

Indian Relations In Utah During The Civil War, Kenneth L. Alford Ph.D.

Faculty Publications

A discussion of native American (Indian) relations in Utah Territory during the Civil War, including the differing policies of Mormon president Brigham Young and U.S. Army commander Colonel Patrick Edward Connor, the January 1863 Bear River Massacre (called the Battle of Bear River at that time), Indian superintendents, treaties, and reservations.

Private Parties, Legislators, And The Government's Mantle: On Intervention And Article Iii Standing, Suzanne B. Goldberg

Faculty Scholarship

This essay takes up questions regarding whether initiative proponents and legislators can defend a law in federal court when the government declines to defend. Looking first at intervention under the Federal Rules of Civil Procedure, I argue that neither has the cognizable interest needed to enter an ongoing lawsuit as a party. Yet even if they are allowed to intervene, these would-be defenders of state or federal law cannot take on the government’s mantle to satisfy Article III because the government’s standing derives from the risk to its enforcement powers, which is an interest that cannot be delegated to others. …

Health Check: Analyzing Trends In West Michigan 2012, Grand Valley State University

Health Check: Analyzing Trends in West Michigan

No abstract provided.

The Establishment And Regulation Of Melanocyte Stem Cells In Zebrafish, Thomas O'Reilly-Pol

All Theses and Dissertations (ETDs)

Stem cells are the cells that regulate the growth and repair of tissues in adult organisms. In this thesis, I sought to develop methodologies to dissect the function and regulation of stem cells during zebrafish melanocyte regeneration.

In the first part of this thesis, I develop a drug based method of ablating melanocytes in the adult zebrafish body. The drug is a copper chelator, neocuproine: NCP) that I show causes death specifically of melanocytes in adult, which allows for regeneration from melanocyte stem cells: MSCs).

In the next part of the thesis, I employ clonal lineage statistical analyses to study …

Natural Killer Cell Recruitment And Activation Following Orthopoxviral Infection, Melissa Anne Pak-Wittel

All Theses and Dissertations (ETDs)

The orthopoxviridae are large, complex DNA viruses with important historical and clinical significance. Members of this genus encode numerous proteins known to subvert the immune response to infection, providing a useful system to probe required host responses for resistance and survival. Natural killer: NK) cells provide an essential innate response to infection through direct lysis of infected cells and cytokine production. The innate anti-viral response following infection is associated with NK cell population expansion in draining LNs, where they are normally a rare population. We have identified mechanisms underlying NK cell expansion following orthopoxviral infection and have examined viral inhibition …

Evolution And Ecology During Domestication In The Neotropical Fruit Tree, Byrsonima Crassifolia (Malpighiaceae), Genevieve Kerry Marie Croft

All Theses and Dissertations (ETDs)

Plant domestication is the process by which people take wild plants into cultivation and shape them over time into reliable food sources enhanced for consumption and propagation via agriculture. It causes genetic, phenotypic, and ecological changes in cultivated populations. This process is frequently studied in domesticated crops. And yet incipiently domesticated taxa, intermediate to wild and domesticated plants, offer unique opportunities to study domestication as it occurs.

In this dissertation, I use the incipiently domesticated Neotropical fruit tree, Byrsonima crassifolia: Malpighiaceae), to study the timing and extent of genetic and ecological changes in a plant with a varied cultivation history …

The Role Of Autophagy And Il-17 In Bone Resorption, Carl Deselm

All Theses and Dissertations (ETDs)

Osteoclasts are essential for skeletal homeostasis: Teitelbaum, 2000). These macrophage-lineage cells function by generating a polarized microenvironment between themselves and bone wherein skeletal matrix is degraded. This resorptive compartment is isolated from the general extracellular space by an actin ring which encompasses the ruffled border, a convoluted plasma membrane structure formed by its fusion with lysosome-related vesicles containing an electrogenic H+ATPase, a chloride channel, LAMP-1, and cathepsin K. In consequence, resorption of bone reflects secretion of HCl to mobilize mineral, and cathepsin K to degrade the collagen-rich organic matrix, into the resorptive space: Stenbeck, 2002; Zhao et al., 2008). We …

Atm Deficiency -- A Multifaceted Defect In Lymphocyte Development, Beth Helmink

All Theses and Dissertations (ETDs)

The generation of a functional antigen receptor gene in developing lymphocytes requires that the second exon be assembled through a process known as V(D)J recombination, a process that necessarily involves the generation and repair of DNA double-strand breaks made by the Rag endonuclease. Double strand breaks incurred during G1 of the cell cycle activate ATM, a PI3-kinase-like kinase that, in response to genotoxic DNA damage, is known to phosphorylate hundreds of proteins with unique and diverse functions. Notably, deficiencies in ATM lead to ataxia-telangiectasia, a syndrome characterized by lymphopenia, genomic instability and a predisposition to tumors involving antigen receptor loci …

Establishing Winter Origins Of Migrating Lesser Snow Geese Using Stable Isotopes, Viviane Hénaux, Larkin A. Powell, Mark P. Vrtiska, Keith A. Hobson

School of Natural Resources: Faculty Publications

Increases in Snow Goose (Chen caerulescens) populations and large-scale habitat changes in North America have contributed to the concentration of migratory waterfowl on fewer wetlands, reducing resource availability, and enhancing risks of disease transmission. Predicting wintering locations of migratory individuals is critical to guide wildlife population management and habitat restoration. We used stable carbon (δ¹³C), nitrogen (δ ¹⁵N), and hydrogen (δ²H) isotope ratios in muscle tissue of wintering Snow Geese to discriminate four major wintering areas, the Playa Lake Region, Texas Gulf Coast, Louisiana Gulf Coast, and Arkansas, and infer the wintering locations …

Insight Into The Reactivity Of Metastasis Inhibitor, Imidazolium Trans-[Tetrachloro (Dimethyl Sulfoxide)(Imidazole)Ruthenate(Iii)], With Biologically-Active Thiols, Risikat Ajibola Adigun

Dissertations and Theses

Imidazolium trans-[tetrachloro (dimethyl sulfoxide)(imidazole)ruthenate(III)], NAMI-A, is an experimental metastasis inhibitor whose specific mechanism of activation and action remains to be elucidated. In the nucleophilic and reducing physiological environment; it is anticipated that the most relevant and available reductants upon introduction of NAMI-A as a therapeutic agent will be the biologically-relevant free thiols. The kinetics and mechanisms of interaction of NAMI-A with biologically-active thiols cysteamine, glutathione, cysteine and a popular chemoprotectant, 2-mercaptoethane sulfonate (MESNA) have been studied spectrophotometrically under physiologically-relevant conditions. The reactions are characterized by initial reduction of NAMI-A with simultaneous formation of dimeric thiol and subsequent ligand exchange with …

Atheist Scripts In A Nation Of Religiosity: Identity Politics Within The Atheist Movement, Jacqueline Frost

Dissertations and Theses

This thesis explores the use of identity politics within the atheist movement at both the national and individual levels. I conducted a content analysis of two national atheist groups and three best-selling atheist authors in order to assess the use of atheist identity politics at the national level. I then conducted 15 in-depth interviews with a sample of atheists in Portland, Oregon about their atheist identity and their reactions to and identification with national atheist movement strategies. Findings suggest that national atheist organizations and atheist authors are using a strategy of identity politics that encourage atheists to "come out" as …

At The End Of The Peninsula, Jonathan David Fine

Dissertations and Theses

In 1865, a settler named James John laid out a small neighborhood at the end of the north Portland peninsula, near the confluence of the Willamette and Columbia Rivers. For a half century, until its annexation to Portland in 1915, St. Johns was an independent municipality. Factories lined the waterfront, and a full complement of businesses in the small downtown area--grocers, bakers, hardware stores, clothing shops--catered to all the residents' needs. St. Johns was always a working-class town with a strong sense of identity. But after World War II, as Portland grew, St. Johns began to seem defined less by …

Designing New Drugs To Treat Cardiac Arrhythmia, Yanping Ye

Dissertations and Theses

Heart failure resulting from different forms of cardiomyopathy is defined as the inability of the heart to pump sufficient blood to meet the body's metabolic demands. It is a major disease burden worldwide and the statistics show that 50% of the people who have the heart failure will eventually die from sudden cardiac death (SCD) associated with an arrhythmia. The central cause of disability and SCD is because of ventricular arrhythmias. Genetic mutations and acquired modifications to RyR2, the calcium release channel from sarcoplasmic reticulum, can increase the pathologic SR Ca2+ leak during diastole, which leads to defects in SR …

Late-Holocene Flood History, Flood-Frequency, And Paleoclimate Analysis Of The Central Black Hills, South Dakota, Tessa M. Harden

Dissertations and Theses

Stratigraphic records in four basins in the central Black Hills in combination with hydraulic calculations show that all basins have experienced multiple large floods in the last 2,000 years with flow rates substantially larger than those gaged historically. Flood-frequency analyses for the study reaches account for 29 paleofloods inferred from interpretation of stratigraphic records locally extending back 1,000 to almost 2,000 years. The addition of paleoflood data to the gaged and historical data significantly reduced uncertainties related to flood-frequency. For all study reaches the 95-percent confidence intervals about the low-probability quantile estimates (100-, 200-, and 500-year recurrence-intervals) were reduced by …

Effects Of Reactive Oxygen Species On Life History Traits Of Caenorhabditis Elegans, Samson William Smith

Dissertations and Theses

Evolutionary life history theory predicts that tradeoffs among fitness-related phenotypes will occur as a result of resource limitations and/or physiological constraints. Such tradeoffs are defined as the cost(s) incurred on one component of fitness (e.g., reproduction) by the increased expression of another fitness-related trait (e.g., longevity). Only recently have researchers begun to investigate the mechanistic bases of life history tradeoffs. A recent proposal is that reactive oxygen species (ROS) have a central role in shaping life history traits and tradeoffs. Research on disparate animal taxa has highlighted strong correlations between oxidative stress resistance and fitness-related life history traits, for example. …

Revisiting The Work We Know So Little About: Race, Wealth, Privilege, And Social Justice, Stephanie M. Wildman, Beverly Moran, Margalynne J. Armstrong

Faculty Publications

No abstract provided.

Who Owns An Avatar?: Copyright, Creativity, And Virtual Worlds, Tyler T. Ochoa

Faculty Publications

Today's massively multi-player online role-playing games (MMORPGs) offer their users the ability to create or customize their own avatars with distinctive visual appearances. This Article contends that users who take advantage of that ability are exercising significant creative choices, such that they should be considered the "authors" and copyright owners of their own avatars. The Copyright Act envisions several types of collaborative authorship, including joint authorship, works made for hire, and collective works. None of these models provides a good fit for user-created avatars, because avatars meet some, but not all, of the elements for each model. Here, the two …

Seasonal Variation In Heat Fluxes, Predicted Emissions Of Malodorants, And Wastewater Quality Of An Anaerobic Swine Waste Lagoon, Rezaul Mahmood

School of Natural Resources: Faculty Publications

No abstract provided.

Crime And Enforcement In Immigrant Neighborhoods: Evidence From New York City, Garth Davies, Jeffrey Fagan

Faculty Scholarship

Immigration and crime have received much popular and political attention in the past decade, and have been a focus of episodic social attention for much of the history of the U.S. Recent policy and legal discourse suggests that the stigmatic link between immigrants and crime has endured, even in the face of evidence to the contrary. This study addresses the relationship between immigration and crime in urban settings, focusing on areal units where immigrants tend to cluster spatially as well as socially. We ask whether immigration creates risks or benefits for neighborhoods in terms of lower crime rates. The question …

Aging Phenotypes Of Common Marmosets (Callithrix Jacchus), Corinna N. Ross, K. Davis, G. Dobek, S. D. Tardif

Biology Faculty Publications (Archived)

Characterizing the phenotypic changes associated with aging in a short-lived primate is necessary in order to develop better translational models for human health, aging, and disease research. A population of conventionally housed marmoset monkeys was assessed to determine if phenotypes of body composition, hematology, and morphometrical measures were associated with age or risk of death. We found that the cause of mortality in older marmosets was more likely to be due to cardiac and chronic kidney disease than in younger marmosets. Older marmosets have decreased fat mass, morphometric measures, and serum albumin. Older marmosets are more likely to show a …

Humor-Related Social Exchanges And Mental Health In Assisted Living Residents, Ann Elizabeth Mcqueen

Dissertations and Theses

Social contact is known to be vital for older adults' mental and physical health, but few studies of social interactions have taken place in long-term care settings. The current study investigated whether the psychological well-being of assisted living residents was influenced by factors associated with residents' social interactions involving humor. Specific aims of the present study were to develop and test a measure related to humor-related social exchanges, to examine how humor-related social exchanges affect residents' mental health, and to explore whether humor-related social exchanges mediated the effects of resident and facility characteristics on indices of mental health. One hundred …

Site Fidelity, Associations And Long-Term Bonds Of Short-Finned Pilot Whales Off The Island Of Hawai`I, Sabre D. Mahaffy

Dissertations and Theses

Short-finned pilot whales (Globicephala macrorhynchus) are the most frequently sighted odontocete in a long-term study in Hawaiʹi (representing 23.8% of all odontocete sightings from directed research efforts), yet little has been published on this species in Hawaiian waters. Studies elsewhere have suggested that short-finned pilot whales travel in stable mixed-sex groups composed of strongly associated individuals; however temporal analyses of social structure are lacking. To examine site fidelity, association patterns and temporal relationships, I analyzed data from 267 directed research and opportunistic encounters of short-finned pilot whales off the island of Hawai`i from 2003 through 2007. Sightings occurred year-round. Analysis …

Plasma Levels Of Polychlorinated Biphenyls, Non-Hodgkin Lymphoma, And Causation, Michael D. Freeman, Sean S. Kohles

Mechanical and Materials Engineering Faculty Publications and Presentations

Polychlorinated biphenyls (PCBs) are synthetic chlorinated hydrocarbons that have extensively polluted the environment and bioaccumulated in the food chain. PCBs have been deemed to be probable carcinogens by the Environmental Protection Agency, and exposure to high levels of PCBs has been consistently linked to increased risk of non-Hodgkin lymphoma (NHL). In the present article we present a forensic epidemiologic evaluation of the causal relationship between NHL and elevated PCB levels via application of the Bradford-Hill criteria. Included in the evaluation is a meta-analysis of the results of previously published case-control studies in order to assess the strength of association between …

Ultrasonic Wave Propagation Assessment Of Native Cartilage Explants And Hydrogel Scaffolds For Tissue Engineering, Sean S. Kohles, Shelley S. Mason, Anya P. Adams, Robert J. Berg, Jessica Blank, Fay Gibson, Johnathan Righetti, Lesha S. Washington, Asit K. Saha

Mechanical and Materials Engineering Faculty Publications and Presentations

Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including …

Long-Term Responses Of Phalaris Arundinacea And Columbia River Bottomland Vegetation To Managed Flooding, Tina Schantz Farrelly

Dissertations and Theses

I sought to determine the effect of managed flooding on Phalaris arundinacea L. and other plant species distributions in a large wetland complex, Smith and Bybee Wetlands (SBW), in northwestern Oregon. Altered hydrology has reduced historically high spring flow and prematurely initiated the historic summer drying period at SBW. This alteration has increased the coverage of invasive plants (e.g., P. arundinacea) causing a decrease in native plant cover and thus degrading ecological functions. SBW managers installed a water control structure (WCS) between SBW and the Columbia Slough/River system to impound winter rainfall and thus approximate the ecological benefits that natural …

Short Stories, Alisa Eve Welch

Dissertations and Theses

In these six intertwining fictional short stories, one fateful decision ripples through the lives of multiple generations. Annie is an unmarried young mother during World War II when she leaves her young daughter in the care of a childless couple. When Annie fails to return for the child after days and then years, a new and fragile family is formed only to be tested by Annie's eventual return. The other stories in this collection follow the daughters and granddaughters who have to navigate their own lives in the shadow of this abandonment. Spanning multiple decades, Annie's decision remains a pivotal …

A Historical And Archaeological Study Of The Nineteenth Century Hudson's Bay Company Garden At Fort Vancouver: Focusing On Archaeological Field Methods And Microbotanical Analysis, Elaine C. Dorset

Dissertations and Theses

The Hudson's Bay Company (HBC), a British fur-trading enterprise, created a large garden at Fort Vancouver, now in southwest Washington, in the early- to mid-19th century. This fort was the administrative headquarters for the HBC's activities in western North America. Archaeological investigations were conducted at this site in 2005 and 2006 in order to better understand the role of this large space, which seems incongruous in terms of resources required, to the profit motive of the HBC. Questions about the landscape characteristics, and comments by 19th century visitors to the site provided the impetus for theoretical research of gardens as …