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2012

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Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu Jan 2012

Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu

Turkish Journal of Biology

Glutathione S-transferase (GST) is the one of most widely used affinity tags in biotechnology applications. The present study named a GST gene as the TtGSTz1 gene, characterized with the conserved glutathione binding motif (SSTSWRVRIAL) under a GST zeta subfamily from Tetrahymena thermophila. Phylogenetic analysis of the TtGstz1p protein sequence, with its orthologs from different GST classes, showed that it is a member of the unicellular GSTz monophyletic clade, which is positioned close to the bacterial GSTz clade. Seven codons of the TtGSTz1 gene were first engineered by introducing silent mutations (TAA > CAA or TAG > CAG) to the code for glutamine …


Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya Jan 2012

Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya

Turkish Journal of Biology

In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C. The molecular weight of the phytase from Lactobacillus brevis was estimated as 73 kDa and 34 kDa on SDS-PAGE. The optimum activity of Lactobacillus brevis extracellular and intracellular enzymes emerged at 120 °C, pH 3.4 and at 110 °C, pH 3.4, respectively. The extracellular enzyme from Lactobacillus brevis was 91% stable for 5 …


Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao Jan 2012

Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao

Turkish Journal of Biology

The persistence of endosulfan and intermediate metabolite endosulfan sulfate in the environment and their toxic effects on biota necessitate their removal. This study investigated the bioaugmentation of endosulfan-contaminated soil by fungal inoculant Aspergillus niger ARIFCC 1053. The influence of bioaugmentation by A. niger on endosulfan-contaminated soil was evaluated with the help of change in pH and released chloride, and by thin layer chromatography and gas chromatography analysis. Its effects on soil functionality were monitored by estimating dehydrogenase and arylsulfatase enzyme activities. The endosulfan degradation reached an undetectable level on day 15. The pH of the medium was nearly neutral (6.9) …


Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin Jan 2012

Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin

Turkish Journal of Biology

Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.


Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin Jan 2012

Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin

Turkish Journal of Biology

Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and …


Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi Jan 2012

Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi

Turkish Journal of Biology

Hyperthyroidism increases the vulnerability of the heart to ischemia-reperfusion (I/R) injury. Regarding the fact that diazepam also affects cardiac I/R injury, the current study was conducted to investigate the effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart. The animals were divided into 6 groups: the control, hyperthyroid, control diazepam (1 mg/kg), hyperthyroid diazepam (1 mg/kg), control diazepam (5 mg/kg), and hyperthyroid diazepam (5 mg/kg) groups. Langendorff-perfused rat hearts underwent 40 min of global ischemia and 45 min of reperfusion. Different cardiac parameters, including the left ventricular developed pressure, heart rate, coronary flow, and rate pressure product (RPP), were measured. …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Biology

No abstract provided.


Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz Jan 2012

Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz

Turkish Journal of Biology

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in …


Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur Jan 2012

Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur

Turkish Journal of Biology

In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class …


Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural Jan 2012

Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural

Turkish Journal of Biology

Twenty-seven aqueous extracts obtained from 21 plants used in the treatment of respiratory tract infections as folk remedies in Turkey were investigated for their relative total phenolic contents and antioxidant, antibacterial, antimycobacterial, and antifungal activities. Antibacterial and antifungal activities against dermatophytes and yeast-like fungi were determined using the microdilution method. Antibacterial activity of all extracts was more pronounced against gram-positive bacteria than against gram-negative bacteria. These extracts also exhibited higher antifungal activity against Candida krusei than ketaconazole. Six extracts displayed antidermatophytic activity against Epidermophyton floccosum, while only 3 extracts were highly active against Trichophyton rubrum. The extracts showed strong activity …


Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem Jan 2012

Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem

Turkish Journal of Biology

An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified 2.09-fold to homogeneity with a yield of 18.4%. The enzyme had a molecular mass of 31 kDa and a specific activity of 14.122 U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants K_m and V_{max} determined for EG, with carboxyl methyl cellulose as a substrate, were 8.5815 mg CMC/mL and 20.121 U/mg protein, respectively. EDTA increased EG activity by 35% at …


Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun Jan 2012

Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun

Turkish Journal of Biology

Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a …


Effect Of Row Spacing On Seed And Forage Yield In Sainfoin (Onobrychis Viciifolia Scop.) Cultivars, Vladeta Stevovic, Rade Stanisavljevic, Dragan Djukic, Dragan Djurovic Jan 2012

Effect Of Row Spacing On Seed And Forage Yield In Sainfoin (Onobrychis Viciifolia Scop.) Cultivars, Vladeta Stevovic, Rade Stanisavljevic, Dragan Djukic, Dragan Djurovic

Turkish Journal of Agriculture and Forestry

A field experiment including 3 sainfoin cultivars (Makedonka, EG Norm, and the Sokobanja population) was set up in a seed production trial with the aim of determining adequate row spacing for seed and forage yield. The cultivars were sown at 3 different row spacings (20 cm, 50 cm, and 80 cm) and at a within-row spacing of 1 cm. Seed yield was determined over a 3-year period, whereas forage yield and quality were evaluated from the 2nd cut in the 2nd and 3rd years. Crop thinning through the years, distinct competitive ability of sainfoin, and a large impact of weather …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Biology

No abstract provided.


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Biology

No abstract provided.


Moringa Oleifera Leaf Extract: An Innovative Priming Tool For Rangeland Grasses, Wasif Nouman, Muhammad Tahir Siddiqui, Shahzad Maqsood Ahmed Basra Jan 2012

Moringa Oleifera Leaf Extract: An Innovative Priming Tool For Rangeland Grasses, Wasif Nouman, Muhammad Tahir Siddiqui, Shahzad Maqsood Ahmed Basra

Turkish Journal of Agriculture and Forestry

Moringa oleifera leaf extract (MLE) is rich in amino acids, ascorbate, zeatin, minerals, and many other compounds known for their growth-promoting potential. This study was planned to explore the potential of MLE as a seed priming agent to increase the germination rate and plant vigor of 3 range grasses, i.e. Cenchrus ciliaris, Panicum antidotale, and Echinochloa crusgalli. The priming strategies used were hydropriming, CaCl_2, PEG-8000 (-1.1 M Pa), MLE (concentrate; 1:10, 1:20, 1:30, and 1:40 dilutions with distilled water), and matripriming using saturated jute mat for 24 h. The primed seeds were placed between 2 moist Whatman No. 1 filter …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.


An Evaluation Of Seed Spacing Accuracy Of A Vacuum Type Precision Metering Unit Based On Theoretical Considerations And Experiments, İsmet Önal, Adnan Deği̇rmenci̇oğlu, Arzu Yazgi Jan 2012

An Evaluation Of Seed Spacing Accuracy Of A Vacuum Type Precision Metering Unit Based On Theoretical Considerations And Experiments, İsmet Önal, Adnan Deği̇rmenci̇oğlu, Arzu Yazgi

Turkish Journal of Agriculture and Forestry

Currently the most widely used machine for precision seeding of cotton and maize seed is vacuum type. The capture of seeds by vacuum plate and the release of seeds from the vacuum plate should be performed precisely without missing or doubling. The physical phenomena should be clarified theoretically to understand how the precision seeding mechanism works. To solve these problems, an attempt was made to develop a nomogram using equations describing the technical characteristics of the seeder used in this study and to describe the seed capture mechanism relying on basic principles of fluid mechanics and aerodynamic properties of seeds. …


The Effect Of Cadmium On Growth And Antioxidant Responses In The Safflower (Carthamus Tinctorius L.) Callus, Shahram Namjooyan, Ramezanali Khavarinejad, Franceous Bernard, Shahrokh Namdjoyan, Hamed Piri Jan 2012

The Effect Of Cadmium On Growth And Antioxidant Responses In The Safflower (Carthamus Tinctorius L.) Callus, Shahram Namjooyan, Ramezanali Khavarinejad, Franceous Bernard, Shahrokh Namdjoyan, Hamed Piri

Turkish Journal of Agriculture and Forestry

Safflower (Carthamus tinctorius L.) callus cultures grown in the presence of different concentrations (0-100 µM) of cadmium were assayed for growth, Cd accumulations, and antioxidative responses. Cadmium inhibited the growth of calli by 25% and 46% at 75 and 100 µM Cd concentrations, respectively. Cadmium concentration in the calli increased with an increased Cd supply in the growth medium. Calli accumulated 334 mg Cd kg^{-1} of their dry weight at 100 µM Cd. Cadmium induced oxidative stress, which was indicated by modulating antioxidant (glutathione and \alpha-tocopherol) levels and antioxidative enzymes. The effect of Cd on glutathione (GSH) was dose-dependent. The …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.


Mating System In Natural Populations Of Taurus Cedar (Cedrus Libani A.Rich.), Behi̇ye Banu Bi̇lgen, Yusuf Kurt, Nuray Kaya Jan 2012

Mating System In Natural Populations Of Taurus Cedar (Cedrus Libani A.Rich.), Behi̇ye Banu Bi̇lgen, Yusuf Kurt, Nuray Kaya

Turkish Journal of Agriculture and Forestry

The aim of this study was to estimate patterns of the mating system in 2 natural Cedrus libani A.Rich. populations in Elmalı, Antalya, with the aid of isozyme markers. Isozyme analysis was performed on both maternal and embryo tissues of the seeds collected from Avlankuzu and Çamkuyusu populations. Eleven loci encoding 8 enzyme systems were analyzed. The Avlankuzu and Çamkuyusu populations were a homogeneous and similar gene pool for the loci studied. Based on a multilocus estimator, the proportion of viable seeds originating from outcrossing (tm) was 0.940 for the Avlankuzu population and 0.944 for the Çamkuyusu population. In other …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.


Effects Of Low-Molecular-Weight Organic Acids On Phosphorus Sorption Characteristics In Some Calcareous Soils, Neda Moradi, Mirhasan Rasouli Sadaghiani, Ebrahim Sepehr, Babak Abdollahi Mandoulakani Jan 2012

Effects Of Low-Molecular-Weight Organic Acids On Phosphorus Sorption Characteristics In Some Calcareous Soils, Neda Moradi, Mirhasan Rasouli Sadaghiani, Ebrahim Sepehr, Babak Abdollahi Mandoulakani

Turkish Journal of Agriculture and Forestry

Understanding the role of organic acids in phosphorus sorption in soils is very important for economic and environmentally friendly management of soil P. Thus, calcareous surface soils (0-30 cm) from West Azerbaijan Province, Iran, were sampled to study the effect of different organic acids on P sorption. Soil samples (2.5 g) were equilibrated with 25 mL of 0.01 M CaCl_2 solution containing 0-20 mg P L^{-1} and 5 mmol L^{-1} of different organic acids (citric, oxalic, and malic acid). The sorption data were well fitted to Freundlich and linear equations but not to the Langmuir equation. The standard P requirements …


Effects Of Compatibilizer And Nanolayered Silicate On Physical And Mechanical Properties Of Pp/Bagasse Composites, Behzad Kord Jan 2012

Effects Of Compatibilizer And Nanolayered Silicate On Physical And Mechanical Properties Of Pp/Bagasse Composites, Behzad Kord

Turkish Journal of Agriculture and Forestry

Bagasse, an agricultural waste, was investigated for wood plastic nanocomposite production. The incorporation of nanolayered silicate has been considered in recent years for improving the properties of thermoplastic composite. In this study a composite of polypropylene (PP) and bagasse fiber (BF), with different concentrations of nanoclay and maleic anhydride (MA) as compatibilizer, were fabricated by injection molding. Then physical and mechanical properties of the composites were tested according to ASTM standards. The results indicated that tensile modulus and flexural strength increased with an increase in clay loading; however, impact strength and water absorption decreased with an increase in nanolayered silicates. …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.


Morphological And Physiological Differences Between Bareroot And Container Juniperus Excelsa Seedlings, Ayşe Deli̇göz Jan 2012

Morphological And Physiological Differences Between Bareroot And Container Juniperus Excelsa Seedlings, Ayşe Deli̇göz

Turkish Journal of Agriculture and Forestry

The effects of stock type on morphological and physiological characteristics were compared in 1 + 0 Crimean juniper (Juniperus excelsa Bieb.) seedlings. In October 2010, the morphological characteristics of both stock types were determined. The physiological condition of both stock types was evaluated by measuring water relation, root growth potential (RGP), and root electrolyte leakage (REL) parameters in seedlings at periodic intervals from October 2010 to April 2011. Container seedlings had greater root collar diameter, higher height, and greater shoot dry weight than bareroot seedlings, but these differences were not significant. However, the shoot-to-root ratios of the container seedlings were …


Cover And Contents Jan 2012

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.


Determination Of Intraspecific Nuclear Dna Content Variation In Common Vetch (Vicia Sativa L.) Lines And Cultivars Based On Two Distinct Internal Reference Standards, İskender Ti̇ryaki̇, Meti̇n Tuna Jan 2012

Determination Of Intraspecific Nuclear Dna Content Variation In Common Vetch (Vicia Sativa L.) Lines And Cultivars Based On Two Distinct Internal Reference Standards, İskender Ti̇ryaki̇, Meti̇n Tuna

Turkish Journal of Agriculture and Forestry

The 2C nuclear DNA contents of 40 common vetch (Vicia sativa L.) lines and cultivars were determined by using both safflower (Carthamus tinctorius L.) and barley (Hordeum vulgare L.) as internal reference standards in flow cytometry analysis and correlated with plant morphological characters and 1000-seed weight. The t-test results showed a significant difference between the mean DNA values of the barley and safflower standards (t = -16.74, P = 0.0001). Data analysis also indicated significant (P < 0.01) intraspecific nuclear DNA content variation within the common vetch lines and cultivars for both internal standards used. The DNA content values ranged from 3.342 pg to 3.652 pg and from 3.600 pg to 4.002 pg for the internal standards of safflower and barley, respectively. The DNA differences within the internal standards were 0.310 pg and 0.402 pg for safflower and barley, respectively. The internal standard of barley constantly produced higher DNA content values than the values of safflower standard for all vetch lines and cultivars. Nuclear DNA content differences between 2 internal standards for the same plant material reached as high as 15.59% (0.540 pg), which is equivalent to 528.12 Mbp DNA. No significant correlations between 1000-seed weight and the nuclear DNA contents of barley (P < 0.51) or safflower (P < 0.76) were detected.


Comparison Of Hybrid Maize Obtained From Inbred Lines That Are Selected Via Top-Crossing And Discriminant Analysis, Ahmet Öz Jan 2012

Comparison Of Hybrid Maize Obtained From Inbred Lines That Are Selected Via Top-Crossing And Discriminant Analysis, Ahmet Öz

Turkish Journal of Agriculture and Forestry

This research was done at the Black Sea Agricultural Research Institute in Samsun, in 2006 and 2007. The study was conducted to determine the effectiveness of discriminant analysis in determining the parents among inbred lines in hybrid corn breeding. Grain yield and some yield components of the hybrid maize, which was obtained from inbred lines that were selected in accordance with top-crossing and discriminant analysis, was examined in the research. According to the average of 2 years, the average grain yield of 11 maize hybrids obtained via top-crossing was 9160 kg ha^{-1}, whereas the average grain yield of 8 maize …


Evaluation Of Fatty Acid Compositions And Some Seed Characters Of Common Wild Plant Species Of Turkey, Muhammet Tonguç, Sabri̇ Erbaş Jan 2012

Evaluation Of Fatty Acid Compositions And Some Seed Characters Of Common Wild Plant Species Of Turkey, Muhammet Tonguç, Sabri̇ Erbaş

Turkish Journal of Agriculture and Forestry

Seeds of 14 commonly found plant species from 3 different families (Brassicaceae, Dipsacaceae, and Asteraceae) were investigated for 1000-seed weights (g), total oil (%), total protein (%), total soluble sugar contents (TSS; mg g^{-1}), and fatty acid compositions (%). Correlation coefficients of fatty acids from different families were investigated, as well. Palmitic, stearic, oleic, linoleic, linolenic, eicosenoic, and erucic acids were present in varying amounts. Within the family Brassicaceae, only Cardaria draba did not contain eicosenoic and erucic acids, while species of the Asteraceae and Dipsacaceae did not contain linolenic, eicosenoic, or erucic acids. The 1000-seed weights ranged between 0.17 …