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The Role Of Uracil-Dna Glycosylase And Folate In The Repair Of Dna, Sarah Talal Dubaisi

Wayne State University Theses

Abstract

Role of Uracil DNA-Glycosylase and Folate

In the Repair of Oxidative Damage

By

Sarah Dubaisi

May2012

Advisor: Dr. Diane Cabelof

Major: Nutrition and Food Science

Degree: Masters of Science

The impact of DNA damage on genomic integrity has been widely investigated due to its association with cancer and ageing. DNA repair pathways play a critical role in preserving the genomic integrity and protecting the cells against DNA damage caused by oxidative stress and folate deficiency. Base Excision Repair (BER) is one of the major pathways involved in the cellular response to DNA damage, primarily the damage caused by oxidative …

Polymeric Nanocarriers For The Regional And Systemic Delivery Of Therapeutics To And Through The Lungs, Balaji Bharatwaj

Wayne State University Dissertations

The lungs are considered as one of the fastest portals of entry to the bloodstream and oral inhalation (OI) has long been accepted as the preferred mode of administration of therapeutics to the respiratory tract. However, despite its advantages, the lungs have been largely underutilized to target ailments not only of systemic relevance but also several other grave conditions including lung cancer and tuberculosis. Polymeric nanocarriers (PNCs) have several advantages over other drug delivery vehicles including sustained release of moieties, ease of cellular internalization and improved targeting, and hence hold the promise to greatly augment the potential of OI therapies. …

On The Disambiguation Of Meaning: The Effects Of Perceptual Focus And Cognitive Load, Lynne Kennette

Wayne State University Dissertations

Most research supports a non-selective (or exhaustive) account of activation whereby multiple meanings of a word are initially activated (as discussed in Degani & Tokowicz, 2009). But what happens to the non-selected meaning of an ambigious word (e.g., bark) and how is the decision made to select one meaning over the other? A great deal research by Gernsbacher and colleagues suggests that the non-selected meaning is "discarded" via active suppression, but a competing activation-only account is also proposed by Gorfein's research group. The present dissertation examines meaning-selection in ambiguous words using a word to elicit meaning context (rather than a …

Characterization Of Intracellular Interactions Between Dengue Virus And Host Proteins, Dumrong Mairiang

Wayne State University Dissertations

Dengue virus is the causative agent of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of world population live in areas where dengue is prevalent, leading to high levels of morbidity and mortality in many areas. Currently there are no vaccines or effective treatments. The virus is transmitted from one person to another by the yellow fever mosquito, Aedes aegypti. The genome of dengue virus encodes only ten proteins implying that the virus needs to interact with and utilize several host proteins for replication. In this project, I used high-throughput yeast two-hybrid screening to identify mosquito and …

Synthesis Of Oligosaccharide Mimetics By The Desulfurative Rearrangement Of Allylic Disulfides And Stereoelectronic Influence Of C-O Bonds On C- And O-Glycosylation, Myriame Moume-Pymbock

Wayne State University Dissertations

This dissertation focuses on two different problems. The first part of this manuscript presents the application of a novel methodology to access the synthesis of oligosaccharide mimetics while the second part focuses on the study of the stereoelectronic effects of proximal C-O bonds in C- and O-glycosylation reactions.

The first chapter provides a brief overview of the important roles that oligosaccharides and glycoconjugates play in biological processes and sets out the major advances that have been made in the past few decades in the synthesis of such complex structures while bringing out the ongoing challenges and the limitations that impede …

Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells, Jill A. Slater

Wayne State University Dissertations

HYPEROSMOTIC STRESS ENZYME SIGNALING MODULATES OCT4, NANOG,

AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF

MURINE EMBRYONIC STEM CELLS

by

JILL SLATER

MAY 2013

Advisor: Daniel Rappolee, Ph.D.

Major: Physiology

Degree: Doctor of Philosophy

Transcription factor expression and therefore lineage identity in the periimplantation

embryo and its stem cells may be influenced by extracellular stresses,

potentially affecting pregnancy outcome. Cellular stress forces cells to suppress some

normal activities (such as protein synthesis and cell proliferation) in order to repair

stress-damaged macromolecules and restore homeostasis. Therefore, any new

activities that embryonic cells initiate while concurrently funding the demands of the

stress …

The Predicament Of China's "Wto-Plus" Obligation To Eliminate Export Duties: A Commentary On The China-Raw Materials Case, Julia Ya Qin

Law Faculty Research Publications

No abstract provided.

Contracting Blame, Eric A. Zacks

Law Faculty Research Publications

No abstract provided.

Correlation Analyses Of Clinical And Molecular Findings Identify Candidate Biological Pathways In Systemic Juvenile Idiopathic Arthritis, Xuefeng B. Ling, Claudia Macaubas, Heather C. Alexander, Qiaojun Wen, Edward Chen, Sihua Peng, Yue Sun, Chetan Deshpande, Kuang-Hung Pan, Richard Lin, Chih-Jian Lih, Sheng-Yung P. Chang, Tzielan Lee, Christy Sandborg, Ann B. Begovich, Stanley N. Cohen, Elizabeth D. Mellins

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Clinicians have long appreciated the distinct phenotype of systemic juvenile idiopathic arthritis (SJIA) compared to polyarticular juvenile idiopathic arthritis (POLY). We hypothesized that gene expression profiles of peripheral blood mononuclear cells (PBMC) from children with each disease would reveal distinct biological pathways when analyzed for significant associations with elevations in two markers of JIA activity, erythrocyte sedimentation rate (ESR) and number of affected joints (joint count, JC).

Methods

PBMC RNA from SJIA and POLY patients was profiled by kinetic PCR to analyze expression of 181 genes, selected for relevance to immune response pathways. Pearson correlation and Student's t-test …

Hijacked By Statistics, Rescued By Wal-Mart V. Dukes: Probing Commonality And Due Process Concerns In Modern Class Action Litigation, Saby Ghoshray

Loyola University Chicago Law Journal

No abstract provided.

Hyphenated Artists: A Body Of Potential, Laura Reeder

Journal of Social Theory in Art Education

The author of this article, an art teacher, arts education advocate, teaching artist, pre-service art teacher supervisor and instructor confronts “either/or” professional identities in arts education. Multi-faceted artist/scholar/educator/ learner/advocate/personas are “unfenced” in order to navigate spaces of artistic, educational, and cultural production without having to pause for identification at borders. In this form, pedagogies for inventive social change emerge. Dialogue among fields of artists and educators links either/or, artist/teacher qualities in holistic and interdisciplinary descriptions such as artist-teacher, teaching-artist, etc. The hyphenated association has become postmodern shorthand for inclusive “both/and” professional identities that in the 21st century may be limiting …

Deconstructing Events: The Neural Bases For Space, Time, And Causality, Alexander Kranjec, Eileen R. Cardillo, Gwenda L. Schmidt, Matthew Lehet, Anjan Chatterjee

Faculty Publications

Space, time, and causality provide a natural structure for organizing our experience. These abstract categories allow us to think relationally in the most basic sense; understanding simple events requires one to represent the spatial relations among objects, the relative durations of actions or movements, and the links between causes and effects. The present fMRI study investigates the extent to which the brain distinguishes between these fundamental conceptual domains. Participants performed a 1-back task with three conditions of interest (space, time, and causality). Each condition required comparing relations between events in a simple verbal narrative. Depending on the condition, participants were …

Contribution Of The Infection-Associated Complement Regulator-Acquiring Surface Protein 4 (Erpc) To Complement Resistance Of Borrelia Burgdorferi, Claudia Hammerschmidt, Teresia Hallström, Christine Skerka, Reinhard Wallich, Brian Stevenson, Peter F Zipfel, Peter Kraiczy

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Borrelia burgdorferi evades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contribution of CRASP-4 in mediating complement resistance of B. burgdorferi and its interaction with human complement regulators. CRASP-4 (also known as ErpC) was immobilized onto magnetic beads and used to capture proteins from human serum. Following Western blotting, factor H (CFH), CFH-related protein 1 (CFHR1), CFHR2, and CFHR5 were identified as ligands of CRASP-4. To analyze the impact of native CRASP-4 on mediating survival of serum-sensitive cells in human serum, a B. garinii strain was generated …

A New Pneumatology: Comparing Joseph Smith's Doctrine Of The Spirit With His Contemporaries And The Bible, Lynne H. Wilson

BYU Studies

While Joseph Smith's teachings on the Holy Ghost appear to fall within the mainstream of the enthusiastic outbursts of the Second Great Awakening (circa 1800–1840), a closer look shows that his restored doctrines made an abrupt and radical departure from the pneumatology of his day. Many historians interpret Joseph's claim to revelation as a creative response to the cultural and religious stimulus of the "Burned-over District" in upstate New York. Focused research suggests that Joseph Smith's teachings on the Holy Ghost were not merely a product of his environment or a reaction to his culture. His biblical interpretations of the …

Combination Effects Of Docetaxel And Doxorubicin In Hormone-Refractory Prostate Cancer Cells, Eleftheria Tsakalozou, Allison M. Eckman, Younsoo Bae

Pharmaceutical Sciences Faculty Publications

Combination effects of docetaxel (DOC) and doxorubicin (DOX) were investigated in prostate cancer cells (PC3 and DU145). Combination indices (CIs) were determined using the unified theory in various concentrations and mixing ratios (synergy: CI < 0.9, additivity: 0.9 < CI < 1.1, and antagonism: CI > 1.1). DOC showed a biphasic cytotoxicity pattern with the half maximal inhibitory concentration (IC50) at the picomolar range for PC3 (0.598 nM) and DU145 (0.469 nM), following 72 h drug exposure. The IC50s of DOX were 908 nM and 343 nM for PC3 and DU145, respectively. Strong synergy was seen when PC3 was treated with DOC at concentrations lower than its IC50 values (0.125~0.5 nM) plus …

Ceria-Engineered Nanomaterial Distribution In, And Clearance From, Blood: Size Matters, Mo Dan, Peng Wu, Eric A. Grulke, Uschi M. Graham, Jason M. Unrine, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

AIMS: Characterize different sized ceria-engineered nanomaterial (ENM) distribution in, and clearance from, blood (compared to the cerium ion) following intravenous infusion.

MATERIALS & METHODS: Cerium (Ce) was quantified in whole blood, serum and clot (the formed elements) up to 720 h.

RESULTS: Traditional pharmacokinetic modeling showed best fit for 5 nm ceria ENM and the cerium ion. Ceria ENMs larger than 5 nm were rapidly cleared from blood. After initially declining, whole blood 15 and 30 nm ceria increased (results that have not been well-described by traditional pharmacokinetic modeling). The cerium ion and 5 and 55 nm ceria did not …

The Hybrid Laboratory: Informal Spaces For Public-Science Interaction, Whitney Ann Ferrin-Rodriguez

Master's Theses

While growing trends in participatory science and public outreach have made science more accessible to publics, science communities and publics have long interpreted science and laboratories as private domains, perpetuating the idea that science is reserved for "experts" and locations for science are deemed "private." Many studies that examine public-science interaction, however, seldom consider the role of space and place. I argue that material spaces shape public-science interactions. My analysis takes place at an institutionalized informal education platform: a hybrid laboratory: a space used by "expert" astronomers to create new visual knowledge and "lay" publics to actively participate in astronomy. …

Antibodies Against The Envelope Glycoprotein Promote Infectivity Of Immature Dengue Virus Serotype 2, Julia M. Da Silva Voorham, Izabela A. Rodenhuis-Zybert, Nilda Vanesa Ayala Nunez, Tonya M. Colpitts, Heidi Van Der Ende-Metselaar, Erol Fikrig, Michael S. Diamond, Jan Wilschut, Jolanda M. Smit

Open Access Publications

No abstract provided.

Physiological Notch Signaling Maintains Bone Homeostasis Via Rbpjk And Hey Upstream Of Nfatc1, Xiaolin Tu, Jianquan Chen, Joohyun Lim, Courtney M. Karner, Seung-Yon Lee, Julia Heisig, Cornelia Wiese, Kameswaran Surendran, Raphael Kopan, Manfred Gessler, Fanxin Long

Open Access Publications

No abstract provided.

Ligand-Induced Movements Of Inner Transmembrane Helices Of Glut1 Revealed By Chemical Cross-Linking Of Di-Cysteine Mutants, Mike Mueckler, Carol Makepeace

Open Access Publications

No abstract provided.

Effects Of Doxycycline On Gene Expression In Wolbachia And Brugia Malayi Adult Female Worms In Vivo, Ramakrishna U. Rao, Yuefang Huang, Sahar Abubucker, Michael Heinz, Seth D. Crosby, Makedonka Mitreva, Gary J. Weil

Open Access Publications

No abstract provided.

Potential Implications Of Hcn Channel Dysfunction After Subarachnoid Hemorrhage, Ananth K. Vellimana

Open Access Publications

No abstract provided.

Differentiation Of The Lateral Compartment Of The Cochlea Requires A Temporally Restricted Fgf20 Signal, Sung-Ho Huh, Jennifer Jones, Mark E. Warchol, David M. Ornitz

Open Access Publications

No abstract provided.

Constitutively Active Canonical Nf-Kb Pathway Induces Severe Bone Loss In Mice, Jesse E. Otero, Tim Chen, Kaihua Zhang, Yousef Abu-Amer

Open Access Publications

No abstract provided.

Causal Links Between Dorsal Medial Superior Temporal Area Neurons And Multisensory Heading Perception, Yong Gu, Gregory C. Deangelis, Dora E. Angelaki

Open Access Publications

No abstract provided.

Network Analysis Of Corticocortical Connections Reveals Ventral And Dorsal Processing Streams In Mouse Visual Cortex, Quanxin Wang, Olaf Sporns, Andreas Burkhalter

Open Access Publications

No abstract provided.

Functional Relationships Between Lipid Metabolism And Liver Regeneration, David A. Rudnick, Nicholas O. Davidson

Open Access Publications

No abstract provided.

Intestinal Adenocarcinoma Arising In Urinary Conduits, Genevieve C. Moyer, Robert L. Grubb Iii, Frank E. Johnson

Open Access Publications

No abstract provided.

Mhc Class I Cross-Presentation By Dendritic Cells Counteracts Viral Immune Evasion, Katrin Nopora, Caroline A. Bernhard, Christine Ried, Alejandro A. Castello, Kenneth M. Murphy, Peggy Marconi, Ulrich Koszinowski, Thomas Brocker

Open Access Publications

No abstract provided.

Three Ontologies To Define Phenotype Measurement Data, Mary Shimoyama, Rajni Nigam, Leslie Sanders Mcintosh, Rakesh Nagarajan, Treva Rice, D. C. Rao, Melinda R. Dwinell

Open Access Publications

No abstract provided.