Digital Commons Network^™

Electrochemical Analysis Based On Molecular Recognition Of Nucleic Acids And Its Applications, Dian-Ming Zhou, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu

Journal of Electrochemistry

Nucleic acid as the carrier of genetic information and the functional molecules for molecular biology and bioanalytical chemistry has attracted increasing interest in electrochemical analysis. This review presents a brief outline of some electrochemical analytical assays based on molecular recognition of nucleic acids. Most of these methods are focused on the detection of nucleic acid sequence, genetic mutation and nucleic acids as functional molecules.

Fabrication Of Ito Micro-Pore Electrochemical Sensor And The Detection Of Exocytosis, Hui Zhao, Ling Li, Pin-Gang He, Yu-Zhi Fang

Journal of Electrochemistry

Exocytosis is one of the major means for cells to transmit messages. Amperometric detection of exocytosis is an important method in studying communication between cells. In this study a new type of ITO micro-pore electrochemical sensor was fabricated by means of ITO and UV light lithographic mask and was used in the detection of exocytosis with promising results. Compared with the conventional method, the ITO micro-pore sensor can not only replace the carbon fiber electrode, but be combined with fluorescent or chemiluminescent methods in detecting the physiological phenomena of cells.

Surface Diffusion Of Adsorptive Species On Gold Nanoelectrodes, Bao-Fa Su, Wei Wang, Dong-Ping Zhan, Bin Ren, Zhong-Qun Tian

Journal of Electrochemistry

Gold nanoelectrodes were prepared successfully by a programmed laser puller. The Faraday adsorptions of oxygen and iodine, and the underpotential deposition of lead on the gold nanoelectrodes were investigated. The results showed that the active areas of nanoelectrodes were dramatically higher than their appearant geometry areas, which is caused by the surface diffusion of adsorptive species from the nanoscale gold/electrolyte interface to the adjacent gold surface.

Gpsa Student Council Meeting, 8/27/2011, Graduate And Professional Student Association

GPSA Meeting Agendas and Minutes

No abstract provided.

The Murray Ledger And Times, August 27, 2011, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Ripe For The Plucking: Centralized And Consolidated Library Budgets As Revenue Streams For Profit., Roger Cross

Articles

The accumulation and centralization of fiscal and selection power into the hands of library professionals could have benefited all, but inherent flaws were bound to arise. In particular the failure to adapt to increased large profit-driven publishers business. Libraries and universities failed to adequately foresee the extent of publishing monopolization. The academic library, as provider of campus-wide journal subscription, reduced demand by reducing the need to maintain individual departmental and faculty subscriptions. The end result was that a small number of publishers were able to fix pricing according to desired profit margins.

The Daily Gamecock, Friday, August 26, 2011, University Of South Carolina, Office Of Student Media

August

No abstract provided.

Daily Eastern News: August 26, 2011, Eastern Illinois University

August

No abstract provided.

Crystal Structure Of Plasminogen Activator Inhibitor-1 In An Active Conformation With Normal Thermodynamic Stability, Jan K. Jensen, Lawrence C. Thompson, Joel C. Bucci, Poul Nissen, Peter G.W. Gettins, Cynthia B. Peterson, Peter A. Andreasen, J. Preben Morth

Faculty Publications

The serpin plasminogen activator inhibitor-1 (PAI-1) is a crucial regulator in fibrinolysis and tissue remodeling. PAI-1 has been associated with several pathological conditions and is a validated prognostic marker in human cancers. However, structural information about the native inhibitory form of PAI-1 has been elusive because of its inherent conformational instability and rapid conversion to a latent, inactive structure. Here we report the crystal structure of PAI-1 W175F at 2.3 Å resolution as the first model of the metastable native molecule. Structural comparison with a quadruple mutant (14-1B) previously used as representative of the active state uncovered key differences. The …

Flyer News, Vol. 59, No. 1 (Aug. 26, 2011), University Of Dayton

Flyer News

Student-run newspaper of the University of Dayton.

Analysis On Jingtang Container Terminal Port Competition Strategy, Xiaojiao Du

World Maritime University Dissertations

No abstract provided.

Marketing Model Of Freight Forwarding Basing On Information Technology, Yang Li

World Maritime University Dissertations

No abstract provided.

Gulf Coast Faculty Council Minutes - August 26, 2011, Usm Gulf Coast Faculty Council

Gulf Coast Faculty Council Minutes

No abstract provided.

Runx2 Protein Expression Utilizes The Runx2 P1 Promoter To Establish Osteoprogenitor Cell Number For Normal Bone Formation, Julie C. Liu, Christopher J. Lengner, Tripti Gaur, Yang Lou, Sadiq Hussain, Marci D. Jones, Brent Borodic, Jennifer L. Colby, Heather A. Steinman, Andre J. Van Wijnen, Janet L. Stein, Stephen N. Jones, Gary S. Stein, Jane B. Lian

School of Chemical Engineering Faculty Publications

The Runt-related transcription factor, Runx2, is essential for osteogenesis and is controlled by both distal (P1) and proximal (P2) promoters. To understand Runx2 function requires determination of the spatiotemporal activity of P1 and P2 to Runx2 protein production. We generated a mouse model in which the P1-derived transcript was replaced with a lacZ reporter allele, resulting in loss of P1-derived protein while simultaneously allowing discrimination between the activities of the two promoters. Loss of P1-driven expression causes developmental defects with cleidocranial dysplasia-like syndromes that persist in the postnatal skeleton. P1 activity is robust in preosteogenic mesenchyme and at the onset …

Laforin, A Dual Specificity Phosphatase Involved In Lafora Disease, Is Present Mainly As Monomeric Form With Full Phosphatase Activity, Vikas V. Dukhande, Devin M. Rogers, Carlos Romá-Mateo, Jordi Donderis, Alberto Marina, Adam O. Taylor, Pascual Sanz, Matthew S. Gentry

Molecular and Cellular Biochemistry Faculty Publications

Lafora Disease (LD) is a fatal neurodegenerative epileptic disorder that presents as a neurological deterioration with the accumulation of insoluble, intracellular, hyperphosphorylated carbohydrates called Lafora bodies (LBs). LD is caused by mutations in either the gene encoding laforin or malin. Laforin contains a dual specificity phosphatase domain and a carbohydrate-binding module, and is a member of the recently described family of glucan phosphatases. In the current study, we investigated the functional and physiological relevance of laforin dimerization. We purified recombinant human laforin and subjected the monomer and dimer fractions to denaturing gel electrophoresis, mass spectrometry, phosphatase assays, protein-protein interaction assays, …

Leadership Approaches Of Selected Chief Academic Officers In Rural Community Colleges, Kent Hanson

Theses and Dissertations

Rural community colleges face many challenges, especially during times of diminished state funding and calls for greater accountability. The Chief Academic Officer (CAO) leads the academic enterprise of the institution and is responsible for setting the overall direction for program and course delivery. The purpose of this qualitative study was to explore the leadership approaches of rural community college chief academic officers. The following research questions were addressed: How do rural community college chief academic officers perceive their leadership approaches? To what extent does Situational Leadership Theory II (SLT II) apply to rural community college chief academic officers as they …

Racial Differences In Tumor Necrosis Factor-Α-Induced Endothelial Microparticles And Interleukin-6 Production, Michael D. Brown, Deborah L. Feairheller, Sunny R. Thakkar, Praveen Veerabhadrappa, Joon-Young Park

Faculty Publications

African Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels …

Altered Toll-Like Receptor 2-Mediated Endotoxin Tolerance Is Related To Diminished Interferon Beta Production., Ss Zaric, Wa Coulter, Ce Shelburne, Cr Fulton, Ms Zaric, A Scott, Mj Lappin, Dc Fitzgerald, Cr Irwin, Cc Taggart

Peninsula Dental School

Induction of endotoxin tolerance leads to a reduced inflammatory response after repeated challenge by LPS and is important for resolution of inflammation and prevention of tissue damage. Enterobacterial LPS is recognized by the TLR4 signaling complex, whereas LPS of some non-enterobacterial organisms is capable of signaling independently of TLR4 utilizing TLR2-mediated signal transduction instead. In this study we report that Porphyromonas gingivalis LPS, a TLR2 agonist, fails to induce a fully endotoxin tolerant state in a human monocytic cell line (THP-1) and mouse bone marrow-derived macrophages. In contrast to significantly decreased production of human IL-8 and TNF-α and, in mice, …

Uncertain Progress On The Fuzzy Boundaries Of Alzheimer's Disease: Reading Between The Guidelines, Peter J. Whitehouse

Faculty Scholarship

No abstract provided.

August 26, 2011, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

Lawrence University Awarded $552,000 Nsf Grant For Advanced Research Instrumentation, Lawrence University

Press Releases

The largest instrumentation grant in Lawrence University’s history — $552,666 from the National Science Foundation — will fund the purchase of a confocal microscope system to support biological research and strengthen hands-on research training.

Confocal microscopy is a cutting edge technique that provides the best available resolution of microscopic images and allows the reconstruction of three-dimensional structures from images obtained through the microscope. Seven teams of faculty mentors and student researchers — six from Lawrence and one from the University of Wisconsin-Fox Valley — will use the microscope to advance understanding in developmental biology, cell biology, physiology and biochemistry.

Current …

Hrs Review, August 2011, Human Resource Services, Boise State University

HRS Review

No abstract provided.

Water - Activities, Jane Qiong Zhang, Linda Vanasupa

Materials Engineering

The document contains activities for students in higher education. These activities are focused on water issues related to sustainability. It is designed for the general engineering or science audience. Its design is based on the framework of the Fink Taxonomy of Significant Learning. Each activity includes a profile that indicates whether the activity involves direct or indirect information sources, whether the experience is doing or observing and whether it involves individual or group reflection. The profile also includes notes for faculty, an estimate of the time investment, a graphic of the developmental aims with respect to the six areas of …

Population, Jane Qiong Zhang, Linda Vanasupa

Materials Engineering

This document is a set of learning materials to support the teaching of population issues particularly related to sustainability in engineering. It is designed for the general engineering or science audience. Its design is based on the framework of the Fink Taxonomy of Significant Learning. The document is a fully-editable set of slides in open source format (pptx) that includes references to targeted learning objectives on each slide and annotations in the notes section of each slide.

Population - Learning Objectives, Jane Qiong Zhang, Linda Vanasupa

Materials Engineering

This document is a list of learning objectives based on the framework of Fink’s taxonomy of significant learning: foundational knowledge, application, integration, human dimension, caring, and learning how to learn. It represents the aspirations for learning that the population is based on.

Glenn Gates V. Rohm & Haas Co

2011 Decisions

USDC for the Eastern District of Pennsylvania

Water, Jane Qiong Zhang, Linda Vanasupa

Materials Engineering

This document is a set of learning materials to support the teaching of water issues particularly related to sustainability in engineering. It is designed for the general engineering or science audience. Its design is based on the framework of the Fink Taxonomy of Significant Learning. The document is a fully-editable set of slides in open source format (pptx) that includes references to targeted learning objectives on each slide and annotations in the notes section of each slide.

Mph Principles Of Public Health: Fall 2011 Syllabus, Nina Wallerstein, Robert Rhyne, Jonathan Eldredge

Principles of Public Health 2010-2017

No abstract provided.

Innovation Begins With People: Leading With A New Lens, Connie I. Reimers-Hild

Kimmel Education and Research Center: Faculty and Staff Publications

Innovation Begins with People: Leading with a New Lens will focus on developing innovation strategies for communities. Topics covered include leadership, creativity, innovation, teamwork, trends, challenges and opportunities.

Evaluation Of The Global Association Between Cholesterol-Associated Polymorphisms And Alzheimer's Disease Suggests A Role For Rs3846662 And Hmgcr Splicing In Disease Risk, Christopher R. Simmons, Fanggeng Zou, Steven G Younkin, Steven Estus

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: Recent genome-wide association studies (GWAS) have identified single nucleotide polymorphisms (SNP)s that are essentially unequivocally associated with peripheral cholesterol. Since the alleles of the APOE gene, which modulate peripheral cholesterol metabolism, and midlife plasma cholesterol are both associated with Alzheimer's disease (AD) risk, we have evaluated the hypothesis that SNPs associated with plasma cholesterol are also associated with AD.

RESULTS: Seventeen non-APOE SNPs reproducibly associated with cholesterol per GWAS were tested for association with AD in ~2,000 AD and ~4,000 non-AD subjects. As a group, these SNPs are associated with AD. Two SNPs in particular, rs3846662 and rs1532085, are …