Digital Commons Network^™

Solution Phase And Membrane Immobilized Iron-Based Free Radical Reactions: Fundamentals And Applications For Water Treatment, Scott Romak Lewis

University of Kentucky Doctoral Dissertations

Membrane-based separation processes have been used extensively for drinking water purification, wastewater treatment, and numerous other applications. Reactive membranes synthesized through functionalization of the membrane pores offer enhanced reactivity due to increased surface area at the polymer-solution interface and low diffusion limitations. Oxidative techniques utilizing free radicals have proven effective for both the destruction of toxic organics and non-environmental applications. Most previous work focuses on reactions in the homogeneous phase; however, the immobilization of reactants in membrane pores offers several advantages. The use of polyanions immobilized in a membrane or chelates in solution prevents ferric hydroxide precipitation at near-neutral pH, …

The Influence Of Performance Accountability Culture On The Work Of High School Principals, Michael Ian Cohen

Seton Hall University Dissertations and Theses (ETDs)

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Discriminating Microsatellites From Macrophomina Phaseolina And Their Potential Association To Biological Functions, R. S. Arias, J. D. Ray, A. Mengistu, B. E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

One hundred and eighty-two microsatellites or simple-sequence-repeat (SSR) markers for Macrophomina phaseolina were developed. These were tested on 24 isolates of M. phaseolina obtained from seven plant species, and the genetic variation of isolates was studied in relation to potential biological processes that could be affected in this fungus. A total of 120 SSR markers were polymorphic, amplifying >90% of the 24 isolates tested. Thirty percent of the markers showed multiple alleles on individual samples. A large number of markers showed unique alleles in isolates collected from pumpkin and snap bean. DNA sequences corresponding to 43 markers had significant hits …

Field-Scale Soil Property Changes Under Switchgrass Managed For Bioenergy, Marty R. Schmer, M. A. Liebig, K. P. Vogel, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The capacity of perennial grasses to affect change in soil properties is well documented but information on switchgrass (Panicum virgatum L.) managed for bioenergy is limited. An on-farm study (10 fields) in North Dakota, South Dakota, and Nebraska was sampled before switchgrass establishment and after 5 years to determine changes in soil bulk density (SBD), pH, soil phosphorus (P), and equivalent mass soil organic carbon (SOC). Changes in SBD were largely constrained to near-surface depths (0–0.05 m). SBD increased (0–0.05 m) at the Nebraska locations (mean=0.16 Mgm^-3), while most South Dakota and North Dakota locations showed declines …

Community Structures Of Fecal Bacteria In Cattle From Different Animal Feeding Operations, Orin C. Shanks, Catherine A. Kelty, Shawn Archibeque, Michael Jenkins, Ryan Newton, Sandra L. Mclellan, Susan M. Huse, Mitchell L. Sogin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fecal microbiome of cattle plays a critical role not only in animal health and productivity but also in food safety, pathogen shedding, and the performance of fecal pollution detection methods. Unfortunately, most published molecular surveys fail to provide adequate detail about variability in the community structures of fecal bacteria within and across cattle populations. Using massively parallel pyrosequencing of a hypervariable region of the rRNA coding region, we profiled the fecal microbial communities of cattle from six different feeding operations where cattle were subjected to consistent management practices for a minimum of 90 days. We obtained a total of …

Use Of Gis-Based Site-Specific Nitrogen Management For Improving Energy Efficiency, Kevin F. Bronson, Peter C. Scharf, Newell R. Kitchen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To our knowledge, geographical information system (GIS)-based site-specific nitrogen management (SSNM) techniques have not been used to assess agricultural energy costs and efficiency. This chapter uses SSNM case studies for corn (Zea mays L.) grown in Missouri and cotton (Gossypium hirsutum L.) grown in Texas. In five case studies, the impact of SSNM will be compared with blanket N fertilizer recommendations. The five case studies are investigating (I) the impact N on energy produced in cotton production, (2) the impact of variable-rate N for cotton production based on soil nitrate and crop reflectance, (3) the feasibility of variable-rate …

The Experience Of Grief And Loss In Persons Affected By Long Qt Syndrome, David Haynes-Weller

PCOM Psychology Dissertations

No abstract provided.

New Records And Ecological Remarks Regarding The Tribe Stomoxyini (Diptera: Muscidae) From Israel, Günter Müller, Jerome Hogsette, Vasiliy Kravchenko, Edita Revay, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Stomoxyini is a small tribe with only ten genera and 49 known species worldwide (Zumpt 1973) belonging to the Muscidae, one of the largest dipteran. Apart from feeding occasionally on nectar (Müller et al. 2011a), the adult flies of this tribe are obligate blood feeders and can easily be recognized by their conspicuous proboscis adapted for biting and sucking blood (Zumpt 1973). Several members of the tribe, especially Stomoxys and Haematobia species, are major pests of domestic livestock. Two species, Stomoxys calcitrans (Linnaeus 1758) and Haematobia irritans (Linnaeus 1758), cause billions of USD in damages annually in the cattle …

Parent Perceptions Of Preadolescent Giftedness And Self Concept, Letitia Pickel

PCOM Psychology Dissertations

Parent perceptions of preadolescent giftedness with regard to the social-emotional development and self-concept of their children were investigated for this study. This study utilized a qualitative approach through information provided by the parents of 21 preadolescent children, with IQ‘s of 110 and above, who participate in a suburban school districts gifted and talented / enrichment program. Interviews were conducted with each parent and included 7 open-ended questions that were intended to elicit personal views regarding their child‘s social-emotional development and self-concept. Information from these interviews, incorporated with the background and demographic questionnaire were examined for potential themes and patterns with …

Climate Impacts On Agriculture: Implications For Forage And Rangeland Production, R. C. Izaurralde, A. M. Thomson, J. A. Morgan, P. A. Fay, H. W. Polley, J. L. Hatfield

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Projections of temperature and precipitation patterns across the United States during the next 50 yr anticipate a 1.5 to 2°C warming and a slight increase in precipitation as a result of global climate change. There have been relatively few studies of climate change effects on pasture and rangeland (grazingland) species compared to those on crop species, despite the economic and ecological importance of the former. Here we review the literature on responses of pastureland and rangeland species to rising atmospheric CO₂ and climate change (temperature and precipitation) and discuss plant and management factors likely to influence pastureland and rangeland …

Protecting Scientific Integrity: The Commercial Speech Doctrine Applied To Industry Publications, Joanna K. Sax

Faculty Scholarship

Pharmaceutical companies face increasing pressure to bring new treatments to market in order to survive. The economic reality of survival and profits may distort a company’s decision-making process regarding full disclosure on a particular new drug.

Part II of this article analyzes the publication tactics employed by some members of the pharmaceutical industry (hereinafter “industry”) and explains how some of the publications promote misleading information. Part III proposes policy recommendations to require accurate dissemination of the results of clinical trials in order to protect scientific integrity and the public welfare. Part IV of this article addresses whether industry publications are …

Evaluation Of Academic Scientists’ Responses To Situations That Pose A Conflict Of Interest, Joanna K. Sax

Faculty Scholarship

The industry-academy relationship has many benefits, but it also has potential drawbacks, including potential conflicts of interest (e.g., when the profit motives of a private company unduly influence academic responsibilities). To date, policies intended to regulate or manage financial conflicts of interest appear to be unsatisfying and inadequate. The present study examined predictors of the responses of academic scientists and clinicians to hypothetical situations in which financial and other conflicts of interest may arise. Academic scientists and clinicians at five medical schools completed an anonymous survey that included vignettes that posed a potential conflict of interest. Participants indicated the likelihood …

Blood Sugar Sex Magik: A Review Of Postconviction Dna Testing Statutes And Legislative Recommendations, Justin Brooks, Alexander Simpson

Faculty Scholarship

Forty-eight states, the District of Columbia, and the federal government have adopted some form of postconviction DNA testing law. Some significant challenges arise when these laws are applied to cases like Richards, which do not involve rape kits but rather require a broader view of how DNA testing can prove innocence. Furthermore, the laws are not uniform, and in the politically charged atmosphere of criminal lawmaking, some of the laws are poorly thought out. This Article reviews these postconviction statutes from the perspective of practitioners who litigate these cases, while also exploring the major questions that ought to be addressed …

Mechanisms That Regulate Peripheral Immune Responses To Control Organ Specific Autoimmunity, Gerard F. Hoyne

Health Sciences Papers and Journal Articles

The immune system must balance the need to maintain a diverse repertoire of lymphocytes to be able to fight infection with the need to maintain tolerance to self proteins. The immune system places strict regulation over the ability of T cells to produce the major T cell growth factor interleukin 2 as this cytokine can influence a variety of immune outcomes. T cells require the delivery of two signals one through the antigen receptor and a second through the costimulatory receptor CD28. The immune system uses a variety of E3 ubiquitin ligases to target signaling proteins that function downstream of …

Analysis Of Biological Features Associated With Meiotic Recombination Hot And Cold Spots In Saccharomyces Cerevisiae, Loren Hansen, Nak-Kyeong Kim, Leonardo Mariño-Ramírez, David Landsman

Mathematics & Statistics Faculty Publications

Meiotic recombination is not distributed uniformly throughout the genome. There are regions of high and low recombination rates called hot and cold spots, respectively. The recombination rate parallels the frequency of DNA double-strand breaks (DSBs) that initiate meiotic recombination. The aim is to identify biological features associated with DSB frequency. We constructed vectors representing various chromatin and sequence-based features for 1179 DSB hot spots and 1028 DSB cold spots. Using a feature selection approach, we have identified five features that distinguish hot from cold spots in Saccharomyces cerevisiae with high accuracy, namely the histone marks H3K4me3, H3K14ac, H3K36me3, and H3K79me3; …

Genome-Wide Association Studies At The Interface Of Alzheimer’S Disease And Epidemiologically Related Disorders, Christopher Ryan Simmons

University of Kentucky Doctoral Dissertations

Genome-wide association studies (GWAS)s provide an unbiased means of exploring the landscape of complex genetic disease. As such, these studies have identified genetic variants that are robustly associated with a multitude of conditions. I hypothesize that these genetic variants serve as excellent tools for evaluation of the genetic interface between epidemiologically related conditions. Herein, I test the association between SNPs associated with either (i) plasma lipids, (ii) rheumatoid arthritis (RA) or (iii) diabetes mellitus (DM) and late-onset Alzheimer’s disease (AD) to identify shared genetic variants. Regarding the most significantly AD-associated variants, I have also attempted to elucidate their molecular function. …

The Absence Of Abcd2 Reveals A Novel Role For Peroxisomes In The Protection From Metabolic Syndrome, Jingjing Liu

University of Kentucky Doctoral Dissertations

ABCD2 (D2) is a peroxisomal ATP binding cassette (ABC) transporter that is expressed in brain, adrenal and liver. D2 is transcriptionally regulated by key transcriptional factors that control lipid and glucose metabolism. Therefore, we examined its role in adipose tissue. These studies revealed that D2 is highly abundant in adipose tissue and upregulated during adipogenesis. However, D2 deficiency does not affect either adipogenesis or lipid accumulation. An examination of the lipid profile of adipose tissue revealed the accumulation of C20 and C22 fatty acids in D2 deficient (D2‐/‐) mice. When challenged with a diet enriched in erucic acid (C22:1, 10% …

Review Of Nature And Scripture In The Abrahamic Religions: Up To 1700, Jame Schaefer

Theology Faculty Research and Publications

No abstract provided.

Communication Apprehension Vs. Social Phobia And Related Conditions A Correlational Study, Christine M. Donaldson

Electronic Theses and Dissertations

Of all social situations, public speaking is the most prevalent fear in both the general population and among social phobic individuals (Mannuzza, Schneier, Chapman, & Liebowitz, 1995; Stein, Walker, & Forde, 1996). The fear of public speaking is referred to as communication apprehension (CA) by members of the communication field; in other programs of study, this condition has been categorized and conceptualized in a wide variety of ways ranging from stage fright to reticence. Several scholarly fields including communication, social psychology, the health sciences and the social sciences, seek to find an explanation and effective intervention for this prevalent condition. …

The Effects Of Parent-Child Interaction Therapy On Symptoms And Impairment In Young Children With Attention-Deficit/Hyperactivity Disorder, Stephanie M. Wagner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Attention-Deficit/Hyperactivity Disorder (ADHD) is an externalizing disorder beginning in childhood with symptoms and impairment persisting into adulthood for many individuals. Although ADHD is identifiable and diagnostically valid during the preschool years, the majority of the research has focused on treating school-aged children. Some research suggests that behavioral parent training (BPT) programs are efficacious in treating young children. Yet, results are inconsistent and previous research has methodological limitations that restrict interpretations of the data. Parent-Child Interaction Therapy (PCIT), a widely-disseminated evidence-based BPT program for young children with disruptive behavior problems, incorporates components (e.g., in vivo coaching) that may be particularly effective …

Rna In Forensic Science: Novel Techniques For Biological Evidence, Stephanie T. Young

Graduate Theses, Dissertations, and Problem Reports (ETD)

The techniques currently utilized for collecting biological evidence at the scenes of violent crimes such as murder, rape, and kidnapping are employed simply to preserve evidence for future DNA analysis. Although DNA is extremely useful in forensic casework, biological samples possess yet another valuable molecule, RNA. The use of RNA in forensic analysis of samples is currently on the rise. In the past, RNA was thought to degrade much too quickly to be of any benefit to the forensic community. The work presented in this dissertation seeks to demonstrate otherwise. While true that RNA is not as stable as DNA, …

De Novo Asymmetric Synthesis Of Biologically Active Carbohydrate Natural Products And Related Sar Studies, Bulan Wu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The carbohydrate portion of natural products plays an important role in its biological activity, such as solubility, target binding, tissue targeting, and membrane transportation. Over the last fourteen years, Dr. O'Doherty's group has been developing de novo methodologies to build the desired carbohydrate functionality and stereochemistry within each sugar from simple achiral starting materials in stark contrast to the traditional carbohydrate approach using known sugar isomers as starting materials. The methodologies depend on a highly diasteroselective palladium-catalyzed glycosylation reaction to control the anomeric stereochemistry, and a highly enantioselective Noyori reduction to install the sugar absolute stereochemistry. Appropriate post-glycosylation transformations create …

Subcellular Distribution Of Apoptosis Associated Speck-Like Protein Mediates Inflammasome Assembly: A Novel Mechanism In The Regulation Of Interleukin-1beta Release, Nicole B. Bryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Inflammation is an essential component of the innate immune defense against pathogens. The process of inflammation is mediated by the rapid release of intercellular mediators, which function in the activation and recruitment of cellular components of both the innate and adaptive immune system. However, dysregulation of this process, resulting in sustained inflammation is the underlying cause of a number of common pathological conditions. These include hereditary autoinflammatory disorders, gout, arthritis, asthma, and cancer. Furthermore, IL-1beta is a key component of the tumor microenvironment, which serves to recruit tumor-associated macrophages, promote angiogenesis, enhance NF-kB activation, and suppress anti-tumor immunity. The studies …

Two-Dimensional Polymer And Thin-Film Semiconductor-Based Photonic Crystals For Biosensing Applications, Bashar M. Hamza

Graduate Theses, Dissertations, and Problem Reports (ETD)

Detecting biomolecules at very low concentrations is of a significant importance for a wide variety of applications ranging from human health to national security. A diverse class of sensing platforms utilizing the specificity of physical properties of materials and their change in the presence of target analytes has been developed. The main objective of such systems is to deliver cost-effective, ultrasensitive, and reliable sensors that can withstand noisy environments (i.e. dirty samples) with efficient operational characteristics (low power, high throughput, etc.). Optical, electrochemical, and mechanical sensors have demonstrated promising detection capabilities, which further encouraged research aimed at producing even much …

Androgen Increases Angiotensin Receptor Type 1a On Smooth Muscle Cells To Promote Angiotensin Ii-Induced Abdominal Aortic Aneurysms, Xuan Zhang

University of Kentucky Doctoral Dissertations

The purpose of this study was to determine whether androgen promotes AT1aR expression on smooth muscle to confer high prevalence of AngII-induced AAAs in hyperlipidemic mice. In addition, we also investigate the role of androgen in the progression of established AngII-induced AAAs.

First, we sought to examine the role of endogenous androgen in the growth of established AngII-induced AAAs. By castrating male mice, we demonstrated that removal of endogenous androgen significantly decreased the progressive lumen dilation of established AngII-induced AAAs in male ApoE-/- mice, but had no effect on external AAA diameters. These results suggest that androgen contributes to the …

Effects Of Corticosterone And Ethanol Co-Exposure On Hippocampal Toxicity: Potential Role For The Nmda Nr2b Subunit, Tracy Renee Butler

University of Kentucky Doctoral Dissertations

Chronic ethanol (EtOH) exposure produces neuroadaptations within the NMDA receptor system and alterations in HPA axis functioning that contribute to neurodegeneration during ethanol withdrawal (EWD). Chronic EtOH exposure and EWD, as well as corticosteroids, also promote increased synthesis and release of polyamines, which allosterically potentiate NMDA receptor open-channel time at the NR2B subunit. The current studies investigated effects of 10 day EtOH and corticosterone (CORT) co-exposure on toxicity during EWD in rat organotypic hippocampal slice cultures, and alterations in function and/or density of the NR2B subunit of the NMDA receptor that may mediate CORT-potentiation of toxicity during EWD. We hypothesized …

Post-Transcriptional Regulation Of Afp And Igm Genes, Lilia M. Turcios

University of Kentucky Doctoral Dissertations

Gene expression can be regulated at multiple steps once transcription is initiated. I have studied two different gene models, the α-Fetoprotein (AFP) and the immunoglobulin heavy chain (IgM) genes, to better understand post-transcriptional gene regulation mechanisms. The AFP gene is highly expressed during fetal liver development and dramatically repressed after birth. There is a mouse strain-specific difference between adult levels of AFP, with BALB/cJ mice expressing 10 to 20-fold higher levels compared to other mouse strains. BALB/cJ mice express low levels of Zhx2 and thus incompletely repress AFP. Despite differences in steady state AFP mRNA levels in the adult liver …

Efficacy Of Organically Certifiable Materials And Natural Compounds Against Foliar Hemibiotrophic And Necrotrophic Fungi In Cantaloupe And Tomato, Merari Feliciano-Rivera

University of Kentucky Doctoral Dissertations

Kentucky reported a solid 13.1% growth in certified organic land from 1997 to 2002. The relative lack of research on disease management practices in Kentucky consistent with organic regulations is an issue that needs to be addressed to provide more reliable information to local farmers. Thus, the first objective of this research was to investigate the potential disease control obtained with natural, organically certifiable spray materials against Colletotrichum orbiculare in vitro and in vivo. The second objective was to test certifiable spray materials in combinations to identify synergistic interactions. The third objective was to evaluate Organic Material Review Institute …

Fabrication And Study Of Molecular Devices And Photovoltaic Devices By Metal/Dielectric/Metal Structures, Bing Hu

University of Kentucky Doctoral Dissertations

A new class of electrodes with nanometer-scale contact spacing can be produced at the edge of patterned metal/insulator/metal this film structures. A key challenge is to produce insulator layers with low leakage current and have pristine metal contacts for controlled molecular contacts. Atomic layer deposition of high quality Al₂O₃ thin films onto Au electrodes was enabled by surface modification with a self-assembled monolayer of -OH groups that react with a monolayer of trimethylaluminum gas source. Ar ion milling was then used to expose the edge of the Au/dielectric/Au structure for molecular electrode contacts. The junctions are characterized …

Factors Related To Birth Transition Success Of Late-Preterm Infants, Karen L. Wright

Electronic Theses and Dissertations

Problem: Identifying the factors effecting birth transition success of late preterm infants may improve early recognition of newborn compromise. Multiple explanatory variables may be associated with birth transition success or failure. The purpose of the study was to determine the prevalence of, and clinical-epidemiological and demographic predictive factors for birth transition success of late preterm infants. Methods: A retrospective case-control chart review was used to compare the characteristics of successful and unsuccessful birth transition of 35 and 36 week gestational age late-preterm infants delivered in a large tertiary-care center during calendar year 2007. A mixture of categorical and numeric variables …