Digital Commons Network^™

14-3-3Γ Binds To Cam Kkα And Blocks Estrogen Signaling In Mcf-7 Cells, John M. Schmitt, Amanda P. Ankeny

Faculty Publications - Department of Biological & Molecular Science

Abstract Extracellular Signal-Regulated Kinase (ERK) is activated by estrogen (E2) downstream of CaM KK leading to cell growth in MCF-7 breast cancer cells. Previous studies have shown that ERK activation may be inhibited by cAMP and PKA. PKA has numerous cellular targets including CREB, Src, Raf-1, arrestins, and CaM KK. CaM KK is inhibited by direct PKA phosphorylation and the subsequent interaction with 14-3-3γ . Agonists that activate cAMP and PKA may block CaM KK activation of ERK and cell proliferation. Our goal was to evaluate the ability of cAMP and PKA to antagonize the effects of E2 on MCF-7 …

Transcription Factor Regulation Of Erk And Estrogen In Mcf-7 Cells, John M. Schmitt, Jessica N. Magill

Faculty Publications - Department of Biological & Molecular Science

Abstract ERK is activated by increased intracellular calcium downstream of the hormone estrogen (E2). E2 activates ERK via the CaM Kinases, specifically CaM KK and CaM KI in MCF-7 cells. ERK may control cell growth and proliferation through Elk-1, Rsk, SRF, CREB, and numerous other molecules and nuclear targets. Vitamin D, a hormone, has proven to be an effective antagonist of ERK and MCF-7 breast cancer cell growth. Our goal was to evaluate if the E2 pathway working through CaM KK and ERK regulated the transcription factors Elk-1, CREB, and SRF. We also examined the ability of vitamin D to …

Nature In A Box: Ecocriticism, Goethe’S Ironic Werther, And Unbalanced Nature, Heather I. Sullivan

Modern Languages and Literatures Faculty Research

Ecocriticism emphasizes how our bodily and ecological boundaries are just as porous, inter-penetrable, and open as are our cultural and linguistic realms. As individual bodies and communities, we are fully immersed in our material environment and participating in constant exchanges of matter and energy. In this essay, I nevertheless advocate for a cautious approach to the ecocritical question of contested boundaries. After all, some boundaries and membranes are necessary to maintain living organisms. Regarding Timothy Morton’s assertion that we are “radically open,” I note the need for stable and healthy membranes to sustain life, such as our porous yet enclosed …

Affinity Studies And Open Systems: A Non-Equilibrium, Ecocritical Reading Of Goethe's Faust, Heather I. Sullivan

Modern Languages and Literatures Faculty Research

Ecocriticism’s contributions to the current rejection of dualistic thinking are noteworthy, particularly when this interdisciplinary field concentrates on hybridity and “relations” that preexist essences. In this mode, ecocriticism participates in a broader development of “affnity studies” that encompass the many efforts across the disciplines toward reconfiguring our “intraactions” with the world in terms that avoid dichotomies and Newtonian linearity and that utilize instead nonlinear, nondualistic forms of “hybridity.” Hybrids, in Steve Hinchliffe’s words, are “more or less durable bodies made up of similarly hybrid and impermanent relations.

Chemical Evolution And Residence Time Of Groundwater In The Wilcox Aquifer Of The Northern Gulf Coastal Plain, Estifanos Haile

Theses and Dissertations--Earth and Environmental Sciences

This study aims to integrate groundwater geochemistry and mathematical modeling to determine the dominant geochemical processes and groundwater residence time within the Wilcox aquifer in the northern Gulf Coastal Plain. Groundwater samples were collected and analyzed for major ion chemistry, stable isotopes (¹⁸O, ²H, and ¹³C), and radioisotope ³⁶Cl content. Geochemical modeling enabled the identification of major sources and sinks of solutes in the aquifer. A two-dimensional, finite-difference, numerical model was used to determine the deep groundwater flow rate and transport of ³⁶Cl in the aquifer. Major ion chemistry shows a chromatographic pattern along …

Rotate And Hold And Scan (Rahas): Structured Light Illumination For Use In Remote Areas, Eli Ross Crane

University of Kentucky Master's Theses

As a critical step after the discovery of material culture in the field, archaeologists have a need to document these findings with a slew of different physical measurements and photographs from varying perspectives. 3-D imaging is becoming increasingly popular as the primary documenting method to replace the plethora of tests and measurements, but for remote areas 3-D becomes more cumbersome due to physical and environmental constraints. The difficulty of using a 3-D imaging system in such environments is drastically lessened while using the RAHAS technique, since it acquires scans untethered to a computer. The goal of this thesis is to …

Mathematical Modeling Of Clostridium Thermocellum’S Metabolic Responses To Environmental Perturbation, Bless Adotey

Theses and Dissertations--Biosystems and Agricultural Engineering

Clostridium thermocellum is a thermophilic anaerobe that is capable of producing ethanol directly from lignocellulosic compounds, however this organism suffers from low ethanol tolerance and low ethanol yields. In vivo mathematical modeling studies based on steady state traditional metabolic flux analysis, metabolic control analysis, transient and steady states’ flux spectrum analysis (FSA) were conducted on C. thermocellum’s central metabolism. The models were developed in Matrix Laboratory software ( MATLAB^® (The Language of Technical Computing), R2008b, Version 7.7.0.471)) based on known stoichiometry from C. thermocellum pathway and known physical constraints. Growth on cellobiose from Metabolic flux analysis (MFA) and Metabolic …

Moisture Control Methodology For Gas Phase Compost Biofilters, Lucas Dutra De Melo

Theses and Dissertations--Biosystems and Agricultural Engineering

Gas phase biofilters are used for controlling odors from animal facilities. Some characteristics can affect their performance and moisture content is one very important. A methodology for controlling and measuring moisture content is required to optimize these systems. An experiment was conducted to determine the appropriate placement of a set of soaker hoses 1.2 m in length for water application. It was found that the soaker hose installed in the lower region of the biofilter coupled with appropriate and timely application of water was able to minimize drying of the compost. Thermal conductance proved to be a reliable indicator for …

Nigrostriatal Dopamine-Neuron Function From Neurotrophic-Like Peptide Treatment And Neurotrophic Factor Depletion, Ofelia Meagan Littrell

Theses and Dissertations--Neuroscience

Trophic factors have shown great promise in their potential to treat neurological disease. In particular, glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor for midbrain dopamine (DA) neurons in the substantia nigra (SN), which lose function in Parkinson’s disease (PD). GDNF progressed to phase II clinical trials, which did not meet proposed endpoints. The large size and binding characteristics of GDNF have been suspected to contribute to some of the shortcomings of GDNF related to delivery to target brain regions. Smaller peptides derived from GDNF (Dopamine-Neuron Stimulating Peptides – DNSPs) have been recently investigated …

Lysophosphatidic Acid Production And Signaling In Platelets, Zachary Bennett Fulkerson

Theses and Dissertations--Physiology

Lysophosphatidic acid (LPA) belongs to a class of extracellular lipid signaling molecules. In the vasculature, LPA may regulate platelet activation and modulate endothelial and smooth muscle cell function. LPA has therefore been proposed as a mediator of cardiovascular disease.

The bulk of circulating LPA is produced from plasma lysophosphatidylcholine (LPC) by autotaxin (ATX), a secreted lysophospholipase D (lysoPLD). Early studies suggest that some of the production of circulating LPA is platelet-dependent. ATX possesses an N-terminal somatomedin B-like domain suggesting the hypothesis that ATX interacts with platelet integrins which may localize ATX to substrate in the membrane and/or alter the catalytic …

Mechanism And Kinetics Of Covalent Amide-Linked Adduct Formation In Solution And Solid Peptide Formulations, Michael Paul Dehart

Theses and Dissertations--Pharmacy

Peptides and proteins are prone to chemical and physical degradation in solutions and solids. One of the most common chemical degradation pathways involves deamidation of asparagine residues through a reactive succinimide intermediate resulting in the formation of aspartyl and isoaspartyl degradation products.

In this work, kinetic studies in aqueous solutions demonstrated that the succinimide intermediate is susceptible to attack by nucleophiles other than water. This may lead to a variety of alternative degradants. In solutions containing high concentrations of ammonia, the succinimide generated in the deamidation of the model peptides Phe-Asn-Gly and Phe-isoAsn-Gly underwent back-reaction with ammonia leading to peptide …

Viability Of A Controllable Chaotic Micromixer Through The Use Of Titanium-Nickel Shape Memory Alloy, David Ryan Lilly

Theses and Dissertations--Mechanical and Aerospace Engineering

Microfluidic devices have found applications in a number of areas, such as medical analysis, chemical synthesis, biological study, and drug delivery. Because of the small channel dimensions used in these systems, most microchannels exhibit laminar flow due to their low Reynold’s number, making mixing of fluids very challenging. Mixing at this size scale is diffusion-limited, so inducing chaotic flow patterns can increase the interface surface area between two fluids, thereby decreasing overall mixing time.

One method to create a chaotic flow within the channel is through the introduction of internal protrusions into the channel. In such an application protrusions that …

Target Validation Of Uk-101 And Functional Studies Of Β1i, Marie V. Wehenkel

Theses and Dissertations--Pharmacy

β1i is a major catalytic subunit of the immunoproteasome, an alternative form of the constitutive proteasome, and its upregulation has been demonstrated in a variety of disease states including cancer. Our lab has developed a small molecule inhibitor of β1i, dubbed UK-101. While UK-101 causes apoptosis in cancer cell lines, it was not clear whether this apoptotic effect was directly mediated by its irreversible inhibition of β1i. Since off-target effects are major roadblocks for the development of new and effective pharmaceuticals, target validation studies in this system would assist in the further progression of β1i inhibitors towards preclinical trials. Our …

Studies Of Solubilization Of Poorly Water-Soluble Drugs During In Vitro Lipolysis Of A Model Lipid-Based Drug Delivery System And In Mixed Micelles, Lin Song

Theses and Dissertations--Pharmacy

Lipid-based drug delivery systems (LBDDSs) are becoming an increasingly popular approach to improve the oral absorption of poorly-water soluble drugs. Several possible mechanisms have been proposed to explain the means by which LBDDSs act in vivo to enhance absorption. The goal of the current dissertation is to provide a better understanding of one proposed mechanism; the capability of lipoidal components in LBDDS formulations to create and maintain a drug in a supersaturated state under simulated GI conditions. Moreover, molecular details of equilibrium solubilization of a drug in a series of model lipid assemblies were examined. The results of these studies …

Preclinical Evaluation Of Lobeline For The Treatment Of Adhd: Comparison With Psychostimulant Therapies, Yolanda D. Williams

Theses and Dissertations--Pharmacy

This dissertation work investigated the effect of acute and repeated in vivo administration of lobeline on dopamine transporter (DAT) and vesicular monoamine transporter (VMAT2) function. The effects of lobeline were then compared to the effects of acute and repeated in vivo administration of methylphenidate and amphetamine to determine if lobeline produced similar effects compared to these Attention Deficit Hyperactivity Disorder (ADHD) medications. These medications are considered the first line of pharmacotherapy for ADHD, although there is a growing concern associated with their potential for abuse and other side effects. This merits the need for novel ADHD treatments that have a …

Role Of The Sexual Cycle In Development Of Genotypic And Phenotypic Diversity In Gibberella Zeae, Sladana Bec

Theses and Dissertations--Plant Pathology

Gibberella zeae (anamorph Fusarium graminearum) is a homothallic ascomycete pathogen that is responsible for causing Fusarium head blight (FHB) of wheat and small grains. In addition to causing a reduction in yield, harvested grain is frequently contaminated with trichothecene mycotoxins that are harmful for human and animal health. Use of wheat varieties with resistance to FHB is an important strategy to lower its impact. In order to produce varieties with durable resistance, we must understand the origin and degree of genetic diversity present in the pathogen population. In my research, I focused my efforts on an investigation of the …

Status Of A Reintroduced Black Bear Population In The Big South Fork Area Of Kentucky, Sean Mccarthy Murphy

Theses and Dissertations--Forestry and Natural Resources

Large carnivores have been subjected to overexploitation and extensive habitat loss for centuries. Reintroduction has become an increasingly used tool for recovering and reestablishing large carnivore populations; however, most reintroductions have either failed or resulted in small populations that are vulnerable to deleterious demographic, environmental, and genetic effects that can lead to population loss or extinction. Longterm monitoring of small, reintroduced populations is critical to population persistence and viability. To evaluate long-term reintroduction success and current status of a recently reintroduced, small black bear (Ursus americanus) population in the Big South Fork area of Kentucky, I used non-invasive …

Genetic Immunization In The Horse: The Potential For Enhanced Immune Responses With Deacylated Polyethyleneimine (Pei) And Immunostimulatory Cytokines As Vaccine Adjuvants, Deborah Lee Even

Theses and Dissertations--Veterinary Science

DNA vaccines in larger animals, such as horses, are generally less effective and elicit significantly weaker immune responses, than in small animal model systems. To provide optimal protection against pathogenic microorganisms, the induction of both humoral and cellular immune responses from DNA vaccination may be necessary. One limitation to DNA immunization in the horse is the difficulty in generating high levels of antigen-specific antibody and CTL responses. Previous work in the laboratory has demonstrated that expression constructs containing native sequences encoding the surface unit (SU) envelope glycoprotein (pCiSU) of the Equine Infectious Anemia Virus (EIAV) are ineffective at stimulating immune …

Courts' Increasing Consideration Of Behavioral Genetics Evidence In Criminal Cases: Results Of A Longitudinal Study, Deborah W. Denno

Faculty Scholarship

This article, which is part of a symposium honoring David Baldus, presents a unique study of all criminal cases (totaling thirty-three) that addressed behavioral genetics evidence from June 1, 2007, to July 1, 2011. The study builds upon this author’s prior research on all criminal cases (totaling forty-eight) that used such evidence during the preceding thirteen years (1994-2007). This combined collection of eighty-one criminal cases employing behavioral genetics evidence offers a rich context for determining how the criminal justice system has been handling genetics factors for nearly two decades, but also why the last four years reveal particularly important discoveries. …

Writing Spaces: Readings On Writing, Volume 2, Charles Lowe, Pavel Zemliansky

Open Textbooks

Volumes in Writing Spaces: Readings on Writing offer multiple perspectives on a wide-range of topics about writing. In each chapter, authors present their unique views, insights, and strategies for writing by addressing the undergraduate reader directly. Drawing on their own experiences, these teachers-as-writers invite students to join in the larger conversation about the craft of writing. Consequently, each essay functions as a standalone text that can easily complement other selected readings in writing or writing-intensive courses across the disciplines at any level.

Profiling Executive Dysfunction In Adolescents With Autism, Nicole R. Rogers

PCOM Psychology Dissertations

Executive Functions (EF) have been assigned a causative role in a number of disorders, including schizophrenia, Tourette syndrome, autism, obsessive compulsive disorder, violent and criminal behavior, and nearly all learning disabilities. While the term executive functions in the professional literature easily yields more than 150 references, empirical research in which executive functions are studied and linked with specific disorders, such as autism, continues to help answer long-held questions about the disorder and adds to the literature base in order to better understand and treat the disorder. The purpose of this study was to review archival data collected using the Behavior …

Encyclopedia Of Snow, Ice And Glaciers, Vijay P. Singh, Pratap Singh, Umesh K. Haritashya

Department of Earth, Environment and Sustainability Faculty Publications

The objective of this encyclopedia is to present the current state of scientific understanding of various aspects of earth’s cryosphere – snow, glaciers, ice caps, ice sheets, ice shelves, sea ice, river and lake ice, and permafrost – and their related interdisciplinary connections under one umbrella. Therefore, every effort has been made to provide a comprehensive coverage of cryosphere by including a broad array of topics, such as the atmospheric processes responsible for snow formation; snowfall observations; snow cover and snow surveys; transformation of snow to ice and changes in their properties; classification of ice and glaciers and their worldwide …

A Basic Theory Of Benford’S Law, Arno Berger, Theodore P. Hill

Research Scholars in Residence

Drawing from a large, diverse body of work, this survey presents a comprehensive and unified introduction to the mathematics underlying the prevalent logarithmic distribution of significant digits and significands, often referred to as Benford’s Law (BL) or, in a special case, as the First Digit Law. The invariance properties that characterize BL are developed in detail. Special attention is given to the emergence of BL in a wide variety of deterministic and random processes. Though mainly expository in nature, the article also provides strengthened versions of, and simplified proofs for, many key results in the literature. Numerous intriguing problems …

Saplings 2011, Sean M. Goins, Joseph Braner, Amy L. Myers, Charlie Jackson, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Sapling data (woody species >50 centimeters in height and <2.5 cm diameter at breast height) collected in 2011 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

Overstory 2011, Sean M. Goins, Joseph Braner, Amy L. Myers, Charlie Jackson, Albert J. Burky, Julia I. Chapman, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Overstory data (woody species ≥2.5 centimeters in diameter at breast height) collected in 2011 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.

2011 Program And Abstracts, University Of Dayton. Department Of Mathematics

Undergraduate Mathematics Day: Programs, Lectures, Promotional Materials

Program and abstracts of papers presented at the 2011 Undergraduate Mathematics Day held at the University of Dayton.

Perfect And Abundant Numbers - A Perfect And Abundant Source For Undergraduate Research Projects (Abstract), Judy Holdener

Undergraduate Mathematics Day: Programs, Lectures, Promotional Materials

In June of 2009, the 47th perfect number was discovered by the Great Internet Mersenne Prime Search (GIMPS), a collaborative computing project involving volunteers from all over the world. Like all known perfect numbers before it, this number is even. Does an odd perfect number exist? Are there infinitely many perfect numbers?

Can Luce Irigaray's Notion Of Sexual Difference Be Applied To Transsexual And Transgender Narratives?, Danielle Poe

Philosophy Faculty Publications

For over thirty years, Luce lrigaray's work on sexual difference has been the subject of debate about whether sexual difference is essential, necessary, oppressive, or some combination of these. I examine critiques from people who claim that her work is based on an essentialism that is dismissive and harmful to transsexual and transgender discourse. I argue that lrigaray's ethics, based on sexual difference, has the potential to lead to discussions about all difference, including differences in sexuality. lrigaray's complex understanding of sexual difference as natural, cultural, spiritual, and morphological can help us interpret transsexual narratives, narratives by people who seek …

Index Of Titles And Authors, Volumes 1-22

Basic Communication Course Annual

No abstract provided.

Ursinus Magazine, Winter 2011, Wendy Greenberg, Kathryn Campbell, Ellen Labrecque, John E. F. Corson, Jim Wagner, Bobby Fong, Lori G. Piccone, Catherine Chambliss, Robert Strauss

Ursinus Magazine, 1995–Present

First Rhodes Scholar for Ursinus • Periclean faculty leader • Mike McGarvey jersey retired • Faculty books • Community partnership parade • The Philip and Muriel Berman Museum of Art upcoming exhibitions • Ursinus football's Chris Rusiewicz named Assistant Coach of the Year • Sustainability staff • The road ahead: A conversation with Dr. Bobby Fong • Walking across England • Making it work • Summing it up: Professor Catherine Chambliss on mothers, careers, and the balancing act • The gift of bees • Taste tested, mother approved • Behind the lens in Africa • Life in Madagascar • Class …