Digital Commons Network^™

Epistasis Between Comt And Mthfr In Maternal-Fetal Dyads Increases Risk For Preeclampsia, Lori D. Hill, Timothy P. York, Juan P. Kusanovic, Ricardo Gomez, Lindon J. Eaves, Roberto Romero, Jerome F. Strauss Iii

Obstetrics and Gynecology Publications

Preeclampsia is a leading cause of perinatal morbidity and mortality. This disorder is thought to be multifactorial in origin, with multiple genes, environmental and social factors, contributing to disease. One proposed mechanism is placental hypoxia-driven imbalances in angiogenic and anti-angiogenic factors, causing endothelial cell dysfunction. Catechol-O-methyltransferase (Comt)-deficient pregnant mice have a preeclampsia phenotype that is reversed by exogenous 2-methoxyestradiol (2-ME), an estrogen metabolite generated by COMT. 2-ME inhibits Hypoxia Inducible Factor 1α, a transcription factor mediating hypoxic responses. COMT has been shown to interact with methylenetetrahydrofolate reductase (MTHFR), which modulates the availability of S-adenosylmethionine (SAM), a COMT …

Nitric Oxide Synthase Inhibition Enhances The Antitumor Effect Of Radiation In The Treatment Of Squamous Carcinoma Xenografts, Robert J. G. Cardnell, Ross B. Mikkelsen

Radiation Oncology Publications

This study tests whether the nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine (L-NNA), combines favorably with ionizing radiation (IR) in controlling squamous carcinoma tumor growth. Animals bearing FaDu and A431 xenografts were treated with L-NNA in the drinking water. IR exposure was 10 Gy for tumor growth and survival studies and 4 Gy for ex vivoclonogenic assays. Cryosections were examined immunohistochemically for markers of apoptosis and hypoxia. Blood flow was assayed by fluorescent microscopy of tissue cryosections after i.v. injection of fluorospheres. Orally administered L-NNA for 24 hrs reduces tumor blood flow by 80% (pex vivo clonogenic assay compared to …

Flt3l Controls The Development Of Radiosensitive Dendritic Cells In The Meninges And Choroid Plexus Of The Steady-State Mouse Brain, Niroshana Anandasabapathy, Gabriel D. Victora, Matthew M. Meredith

Publications

Antigen-presenting cells in the disease-free brain have been identified primarily by expression of antigens such as CD11b, CD11c, and MHC II, which can be shared by dendritic cells (DCs), microglia, and monocytes. In this study, starting with the criterion of Flt3 (FMS-like receptor tyrosine kinase 3)-dependent development, we characterize the features of authentic DCs within the meninges and choroid plexus in healthy mouse brains. Analyses of morphology, gene expression, and antigen-presenting function established a close relationship between meningeal and choroid plexus DCs (m/chDCs) and spleen DCs. DCs in both sites shared an intrinsic requirement for Flt3 ligand. Microarrays revealed differences …

Synthetic Double-Stranded Rna Induces Innate Immune Responses Similar To A Live Viral Vaccine In Humans, Marina Caskey, François Lefebvre, Ali Filali-Mouhim

Publications

Adjuvants are critical for the success of vaccines. Agonists of microbial pattern recognition receptors (PRRs) are promising new adjuvant candidates. A mechanism through which adjuvants enhance immune responses is to stimulate innate immunity. We studied the innate immune response in humans to synthetic double-stranded RNA (polyinosinic:polycytidylic acid [poly IC] stabilized with poly-l-lysine [poly ICLC]), an agonist for toll-like receptor (TLR) 3, and the cytosolic RNA helicase MDA-5. Transcriptional analysis of blood samples from eight volunteers, after subcutaneous administration of poly ICLC, showed upregulation of genes involved in multiple innate immune pathways in all subjects, including interferon (IFN) and inflammasome signaling. …

Spiking Neurons And The First Passage Problem, Lawrence Sirovich, Bruce Knight

Knight Laboratory

We derive a model of a neuron's interspike interval probability density through analysis of the first passage problem. The fit of our expression to retinal ganglion cell laboratory data extracts three physiologically relevant parameters, with which our model yields input-output features that conform to laboratory results. Preliminary analysis suggests that under common circumstances, local circuitry readjusts these parameters with changes in firing rate and so endeavors to faithfully replicate an input signal. Further results suggest that the so-called principle of sloppy workmanship also plays a role in evolution's choice of these parameters.

Estimating The Amount Of Information Conveyed By A Population Of Neurons, Marshall Crumiller, Bruce Knight, Yunguo Yu, Ehud Kaplan

Knight Laboratory

Recent technological advances have made the simultaneous recording of the activity of many neurons common. However, estimating the amount of information conveyed by the discharge of a neural population remains a significant challenge. Here we describe our recently published analysis method that assists in such estimates. We describe the key concepts and assumptions on which the method is based, illustrate its use with data from both simulated and real neurons recorded from the lateral geniculate nucleus of a monkey, and show how it can be used to calculate redundancy and synergy among neuronal groups.

Student Academic Conference 2011, Minnesota State University Moorhead

Student Academic Conference Abstract Books

Minnesota State University Moorhead Student Academic Conference abstract book.

Escape From Competition: Neighbors Reduce Centaurea Stoebe Performance At Home But Not Away, Ragan Callaway, Lauren Waller, Alecu Diaconu, Robert Pal, Alexandra Collins, Heinz Mueller-Schaerer, John Maron

Biological Sciences

The greater abundance of some exotic plants in their nonnative ranges might be explained in part by biogeographic differences in the strength of competition, but these competitive effects have not been experimentally examined in the field. We compared the effects of neighbors on the growth and reproduction of spotted knapweed (Centaurea stoebe) in Europe, where it is native, and in Montana, where it is invasive. There were strong negative competitive effects of neighboring vegetation on C. stoebe growth and reproduction in Europe. In contrast, identical experiments in Montana resulted in insignificant impacts on C. stoebe. Although the mechanisms that produce …

Review Of Donna V. Jones, The Racial Discourses Of Life Philosophy: Négritude, Vitalism, And Modernity., Gregory Byala

Bryn Mawr Review of Comparative Literature

Donna V. Jones, The Racial Discourses of Life Philosophy: Négritude, Vitalism, and Modernity. New York: Columbia University Press, 2010. 231 pp. ISBN 9780231145480.

Review Of Joseph Carroll, Reading Human Nature: Literary Darwinism In Theory And Practice And Virginia Richter, Literature After Darwin: Human Beasts In Western Fiction, 1859-1939., Carlo Salzani

Bryn Mawr Review of Comparative Literature

Joseph Carroll, Reading Human Nature: Literary Darwinism in Theory and Practice. New York: SUNY Press, 2011. 368 pp. ISBN 9781438435220; Virginia Richter, Literature After Darwin: Human Beasts in Western Fiction, 1859-1939. Basingstoke: Palgrave Macmillan, 2011. 272 pp. ISBN 9780230273405.

The Green Legacy Project: Evaluating Campus Tree Benefits, Joy Fritschle, Gary Coutu, Joan Welch, Gerard D. Hertel

WCU Tree Campus USA Documents

Trees on our campus sequester and store carbon, mitigate urban heat island effects, ameliorate air pollution, reduce stormwater runoff, improve water quality, provide habitat for native birds and other animals, and enhance aesthetics. Adequate planning and management of this valuable resource first requires a sound understanding of existing conditions. To this end, a collaborative group of faculty, staff, and students at West Chester University initiated the Green Legacy Project. We conducted an inventory and assessment of north campus tree resources and developed a GIS management database. Approximately 1900 trees cover 16% of north campus, half of which are non-native species. …

Ampk Directly Inhibits Ndpk Through A Phosphoserine Switch To Maintain Cellular Homeostasis, R. U. Onyenwoke, L. J. Forsberg, L. Liu, Tyisha Williams, O. Alzate, J. E. Brenman

Biology Faculty Research

AMP-activated protein kinase (AMPK) is a key energy sensor that regulates metabolism to maintain cellular energy balance. AMPK activation has also been proposed to mimic benefits of caloric restriction and exercise. Therefore, identifying downstream AMPK targets could elucidate new mechanisms for maintaining cellular energy homeostasis. We identified the phosphotransferase nucleoside diphosphate kinase (NDPK), which maintains pools of nucleotides, as a direct AMPK target through the use of two-dimensional differential in-gel electrophoresis. Furthermore, we mapped the AMPK/NDPK phosphorylation site (serine 120) as a functionally potent enzymatic “off switch” both in vivo and in vitro. Because ATP is usually the most abundant …

Relationships Among Music Listening, Temperament, And Cognitive Abilities Of Four-Year-Old Children, John W. Flohr, Diane C. Persellin, Daniel C. Miller, Harry Meeuwsen

Music Faculty Research

The purpose of the study was twofold: (a) to investigate electrophysiological (EEG) responses in children while listening to two contrasting styles of music, and (b) to investigate the relationship between listening to recorded music and the cognitive abilities of four-year-old children. EEG data were collected on a baseline condition of eyes open. These data were then compared to EEG data produced when children listened to selections of music by Bach, while they listened to selections of rock music, and while they performed a standardized cognitive test of visual closure. Behavioral data were also collected on sex, age, home environment, and …

Aquilegia, Vol. 35 No. 3, Fall 2011: Newsletter Of The Colorado Native Plant Society, Bob Henry, Mo Ewing

Aquilegia - Colorado Native Plant Society

No abstract provided.

Influence Of Season And Sex On Terrapene C. Carolina (Eastern Box Turtle) Movements: An Observation Of A Population In West Virginia, Nathalie Caroline Aall

Theses, Dissertations and Capstones

Environmental conditions influence box turtle movements on multiple temporal scales as they navigate their home ranges. This study focuses on the impacts of seasonal shift and sex on straight-line distances traveled, movements along elevations, and home range sizes in the Eastern Box Turtle, Terrapene c. carolina. Radio telemetry was used to track nine T. c. carolina (5 males; 4 females) from May to October 2010 at Beech Fork State Park in Wayne County, West Virginia. Turtles were tracked once a day at two-day intervals. At each turtle location, microhabitat variables recorded were relative humidity, soil and ground surface temperature, canopy …

Dietary Interventions Against Mammary Glands And Prostate Cancers, Juliana Adedayo Akinsete

Theses, Dissertations and Capstones

Dietary components modulate normal cellular functions and, in cancer cells, alter processes that lead to cancer and/or its progression. The change in the patterns of human food production and consumption over time has contributed to increasing risk for diseases including cancer. Omega-3 and omega-6 fatty acids are classes of essential polyunsaturated fatty acids in the human diet and are required for normal growth and development. Omega-3 and omega-6 fats are thought to be required in the ratio of 1:1 to 1:4 on which humans are thought to have evolved. However, the Western diet has greatly shifted from this ratio. The …

The Perception And Knowledge Of Undergraduate Students About School Psychology, Jessica D. Crislip

Theses, Dissertations and Capstones

The profession of school psychology has changed significantly over the course of its existence. With more positions available than can be filled and the growing need for colleges and universities to produce new generations of school psychologists, it is important to understand what people know about the field. The present study was conducted to examine the relationship between the current level of knowledge that undergraduate students in West Virginia have about the field and the decision to select school psychology as a profession. Participants completed a true/false questionnaire. Results indicated a significant difference in school psychology knowledge between those students …

Mechanisms Of Yttrium Oxide Toxicity In Hek293 Cells, Sravanthi Bodapati

Theses, Dissertations and Capstones

As a non-metal oxide, yttrium oxide (Y2O3) nanoparticles have numerous applications in chemical synthesis, mechanical polishing and as additives to drugs, cosmetics, varnishes and food. Recent data have suggested that these particles are capable of inducing oxidative stress and cytotoxicity in human endothelial cell lines. To examine the potential mechanisms of yttrium oxide toxicity, human embryonic kidney (HEK293) cells were exposed to 1, 5, 10, 50 and 100 μM of Y2O3 nanoparticles for 12, 24, 36 or 48 hr. We hypothesized that exposure of HEK293 kidney cells to Y2O3 nanoparticles would be associated with increased evidence of intracellular oxidative stress …

Evaluation Of Toxicological Effects Of Intra Tracheal Instilled Ceo2 Nanoparticles On The Heart Of Male Sprague-Dawley Rats, Radhakrishna Para

Theses, Dissertations and Capstones

The growing application of cerium oxide nanoparticles (CeO2 NP) in several industrial products is likely to be associated with increased risk of inhalation and exposure. How the inhalation of CeO2 NP may affect cardiac structure and function has to our knowledge, not been examined. To examine whether inhalation of CeO2 NP affects cardiac structure and function, male Sprague Dawley rats underwent a single intra tracheal instillation of nanoparticles (7 mg/kg body weight). Animals were sacrificed 1, 3, 14, and 28 days after instillation and protein isolates from the hearts were examined for the presence of oxidative stress, autophagy and apoptosis. …

Rediscovering The Maryland Darter (Etheostoma Sellare), Tyler Russell Hern

Theses, Dissertations and Capstones

The Maryland darter has not been observed since 1988. Historic populations were located in Deer Creek, Swan Creek, and Gashey’s Run all of which occur in the lower Susquehanna drainage. At these locations, specimens were collected or observed at the lowest riffle of the stream. Some researchers suggest this may be a large river darter. The Conowingo Dam complicates surveying below the facility due to the rapid fluctuations in water levels caused by regulation for power production. Surveying efforts included trawling in the mainstem, and visual surveys in the mainstem, and searching/sampling all historically known locations of Etheostoma sellare. During …

Pitx2 Is Overexpressed In Follicular Cell-Derived Thyroid Cancer And Promotes Thyroid Cancer Proliferation By Regulating Cell Cycle, Yue Huang

Theses, Dissertations and Capstones

Thyroid cancer is the most prevailing malignancy of the endocrine system. Its incidence is rapidly rising at the second fastest rate of all malignancies in the United States, making it a significant health problem. Although the majority of thyroid cancer is slowly-growing and well-differentiated, available treatment options are very limited, and most of them require complete removal of the thyroid gland and surrounding tissues. Patients who have undergone thyroid removal have to take life-long hormone replacement therapy, which is very inconvenient and costly. Therefore, there is an urgent need to develop new treatments for this disease. As a prerequisite for …

Cancer Cachexia And Cardiac Atrophy In The Apcmin/+ Mice Model Of Colon Cancer, Nandini Durga Prasanna Kumar Manne

Theses, Dissertations and Capstones

Cancer cachexia is a muscle wasting condition that occurs in response to a malignant growth in the body. Cachexia is associated with heart failure and is estimated to be the immediate cause of death in about a third of all cancer patients. The purpose of this study was to investigate cardiac atrophy in the APCmin/+ mouse model of colorectal cancer. Compared to age matched C57BL/6 (BL6) mice, APCmin/+ body mass and heart mass were lower at 12 (11.1 Ѡ4.5% and 7.6 Ѡ2.8%, respectively) and 20-weeks (26.1 Ѡ2.5% and 6.0 Ѡ3.8%, respectively) of age (P < 0.05). Immunoblot analysis revealed that these changes in mass were accompanied by increased activation of protein kinase B (Akt Thr 473: 74.4 Ѡ10.9% and 216.0 Ѡ19.6% ; Akt Ser 308: 161.6 Ѡ31.7% and 367.4 Ѡ41.6% at 12- and 20-weeks, respectively, (P < 0.05)), mammalian target of rapamycin (mTOR Ser2448: 23.2 Ѡ13.2% and 44.0 Ѡ16.4% at 12- and 20-weeks, respectively, (P < 0.05)), 5' adenosine monophosphate-activated protein kinase (AMPK: 19.6 Ѡ5.2% and 22.5 Ѡ5.5% at 12- and 20-weeks, respectively, (P < 0.05)) and elevated levels of the autophagy regulator beclin1 (4.7 Ѡ3.3% and 9.5 Ѡ3.0% at 12- and 20-weeks, respectively, (P < 0.05)). No evidence of increased cardiac apoptosis, protein ubiquitination or activation of cardiac caspases or calpains was noted. Taken together, these data suggest that the cardiac atrophy that occurs in the 12- and 20-week old APCmin/+ mouse is relatively modest compared to that seen with other tumor models [1] and is associated with evidence of increased cardiac autophagy.

Effects Of Land Use In The Ohio River Basin On The Distribution Of Coliform And Antibiotic Resistant Bacteria In The Ohio River, Gene Chou

Theses, Dissertations and Capstones

Recent studies indicate that antibiotic resistant bacteria can be useful as indicators of water quality (1, 2, 5, 7, 8, 9, 10). Studies in our laboratory have shown that fecal pollution did not fully explain the distribution or the frequency of antibiotic resistant bacteria in the Ohio River (27, 28). Therefore, it is important to understand the factors that affect the distribution of antibiotic resistant bacteria in aquatic habitat. The purpose of this study was to examine the correlations between land use, water quality, and concentration of antibiotic resistant bacteria in the Ohio River. Mid-channel water samples were collected at …

The Application Of The Lamaze Method In The Management Of Acute Pain: A Comparison Of Non-Pharmacological Pain Management Techniques, Emily Marie Selby-Nelson

Theses, Dissertations and Capstones

Pain is a primary concern in health care. Considering the limitations of pharmacological interventions, identifying the effectiveness of alternative pain management is crucial. Utilizing a pre-post design, different psychological approaches to acute pain management were tested. Participants included 85 college females who were randomly assigned to one of four conditions (Long Lamaze, Brief Lamaze, Acceptance of pain, and a Control group). Participants first underwent an Algometer pain task, then received training or control interactions once a week for four weeks, and were tested again on the pain task. Amount of time participants were able to withstand the pain administered in …

Plesiosaur Body Shape And Its Impact On Hydrodynamic Properties, Courtney D. Richards

Theses, Dissertations and Capstones

Despite the variability of cross-sectional body shape within Plesiosauria, its impact on plesiosaur buoyancy and stability has never been investigated. This study focused on Tatenectes, Cryptoclidus, and Muraenosaurus due to their variable body morphologies. Reconstructions were created based on measurements and photographs from fossil remains. The ability of computer models, based upon the reconstructions, to reach equilibrium after submersion, sink via lung deflation, and recover from a lateral roll was tested. For the computer models, Muraenosaurus was replaced with Thalassomedon, which had a similar morphology. Cryptoclidus and Thalassomedon recovered from submersion faster than Tatenectes. All models achieved negative buoyancy with …

The Little Coal River Improvement Project, West Virginia: An Initial Study Of Sediment, Bacteria And Benthic Macroinvertebrates, Emily Vargo

Theses, Dissertations and Capstones

The Little Coal River, located in southern West Virginia, has undergone many changes over the last few years in an effort to reverse the negative effects of various point source and non-point source pollutions. Marshall University, in cooperation with the West Virginia Department of Environmental Protection, Army Corp of Engineers, and the Coal River Group, has designed and implemented a plan for rehabilitation known as the Little Coal Improvement Project. Several improvement structures were strategically installed within the Little Coal River during the summer of 2007. The functions of these structures include sediment flushing and overall habitat improvement. Monitoring these …

Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor

Theses, Dissertations and Capstones

Blacknose dace (Rhinichthys) are one of the most common cyprinid fishes in eastern North America. They also have been a topic of debate for over 30 years because morphology-based systematics has failed to clearly define their taxa. Taxonomists classify the complex into two species and one subspecies: the eastern form, R. atratulus atratulus; and the western form R. obtusus obtusus, and southern form R. obtusus meleagris. This research uses the mitochondrial cytochrome b gene and genomic RAG 2 gene in a phylogenetic analysis to help clarify species relations according to differences between each current species. Maps have been created to …

Plastron Reduction And Associated Myology In Turtles, And Its Implications For Functional Morphology And Natural History, He Zhu

Theses, Dissertations and Capstones

This study seeks to understand the locomotor implications of plastron reduction in turtles through dissection, description and qualitative and quantitative analysis of muscle masses. A comparative study will be conducted between a turtle species with a reduced plastron (Chelydra serpentina) and a species with a full plastron (Trachemys scripta elegans) to gain a better understanding of how the reduced plastron in snapping turtles affects their myology and gait. Quantitative data on muscular mass and length will be obtained for statistical comparison between the two clades. Data collected will be used to make inferences on gait, stance and natural history.

Predictors Of Postoperative Respiratory Failure In Patients Receiving Anesthesia, Marty Henley

Theses, Dissertations and Capstones

Introduction: Postoperative Respiratory failure has been identified as a prevention quality indicator by The Agency for Health Research and Quality (AHRQ). Postoperative Respiratory Failure is associated with increased length of stay, increased costs and increased morbidity and mortality. Unfortunately, current research is limited regarding scoring methods which are predictive for postoperative respiratory failure. This retrospective case control study was conducted to determine if the various tools of evaluation of risk currently available and to determine if any are predictive of the risk of developing postoperative respiratory failure.

Methodology: The design of this research was a retrospective case control study conducted …

Numeric Index To Faculty Senate Recommendations, Marshall University

Recommendations

No abstract provided.