Digital Commons Network^™

An Evaluation Of The Emerging Interventions Against Respiratory Syncytial Virus (Rsv)-Associated Acute Lower Respiratory Infections In Children, Harish Nair, Vasundhara R. Verma, Evropi Theodoratou, Lina Zgaga, Tanvir Huda, Eric Af Simoes, Peter F. Wright, Igor Rudan, Harry Campbell

Dartmouth Scholarship

Background: Respiratory Syncytial Virus (RSV) is the leading cause of acute lower respiratory infections (ALRI) in children. It is estimated to cause approximately 33.8 million new episodes of ALRI in children annually, 96% of these occurring in developing countries. It is also estimated to result in about 53,000 to 199,000 deaths annually in young children. Currently there are several vaccine and immunoprophylaxis candidates against RSV in the developmental phase targeting active and passive immunization.

Methods: We used a modified CHNRI methodology for setting priorities in health research investments. This was done in two stages. In Stage I, we systematically reviewed …

Effect Of Case Management On Neonatal Mortality Due To Sepsis And Pneumonia, Anita K. M. Zaidi, Hammad A. Ganatra, Sana Syed, Simon Cousens, Anne C. C. Lee, Robert Black, Zulfiqar A. Bhutta, Joy E. Lawn

Department of Paediatrics and Child Health

Background: Each year almost one million newborns die from infections, mostly in low-income countries. Timely case management would save many lives but the relative mortality effect of varying strategies is unknown. We have estimated the effect of providing oral, or injectable antibiotics at home or in first-level facilities, and of in-Patient hospital care on neonatal mortality from pneumonia and sepsis for use in the Lives Saved Tool (LiST). Methods: We conducted systematic searches of multiple databases to identify relevant studies with mortality data. Standardized abstraction tables were used and study quality assessed by adapted GRADE criteria. Meta-analyses were undertaken where …

Activation Of Multiple Apoptotic Pathways In Human Nasopharyngeal Carcinoma Cells By The Prenylated Isoflavone, Osajin, Tsung-Teng Huang, Fu-Guo Liu, Chia-Fong Wei, Chia-Chen Lu, Chang-Chieh Chen, Hung-Chi Lin, David M. Ojcius, Hsin-Chih Lai

All Dugoni School of Dentistry Faculty Articles

Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were explored. Osajin was found to significantly induce apoptosis of NPC cells in a dose- and time-dependent manner. Multiple molecular effects were observed during osajin treatment including a significant loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, enhanced expression of Fas ligand (FasL), suppression of glucose-regulated protein 78 kDa (GRP78), and activation of caspases-9, -8, …

Experimental Tuberculosis In The Wistar Rat: A Model For Protective Immunity And Control Of Infection, Amit Singhal, El Moukhtar Aliouat, Maxime Herve, Vanessa Mathys, Mehdi Kiass, Colette Creusy, Baptiste Delaire, Liana Tsenova, Laurence Fleurisse, Julie Bertout, Luis Camacho, Damian Foo, Hui Chien Tay, Jie Yee Siew, Warda Boukhouchi, Marta Romano, Barun Mathema, Veronique Dartois, Gilla Kaplan, Pablo Bifani

Publications and Research

Background: Despite the availability of many animal models for tuberculosis (TB) research, there still exists a need for better understanding of the quiescent stage of disease observed in many humans. Here, we explored the use of the Wistar rat model for the study of protective immunity and control of Mycobacterium tuberculosis (Mtb) infection.

Methodology/Principal Findings: The kinetics of bacillary growth, evaluated by the colony stimulating assay (CFU) and the extent of lung pathology in Mtb infected Wistar rats were dependent on the virulence of the strains and the size of the infecting inoculums. Bacillary growth control was associated with induction …

Optimization Of Multiplex Pcr For Multiple Locus Variable Number Tandem Repeat Analysis For Salmonella Typhimurium, George Hafez, Remington Turner, Andrea Haskett, Rajesh Nagarajan

College of Arts and Sciences Poster Presentations

Salmonella typhimurium infects an estimated 1.4 million in the United States and kills over five hundred annually.  The current way of categorization, Pulse Field Gel Electrophoresis (PFGE) and antibiotic sensitivity testing, lack the ability to distinguish between some closely related strains.  We are attempting to optimize a new technique of categorization, Multiple Locus Variable number tandem repeat Analysis (MLVA).  We made changes to temperature and concentration variables within the multiplex PCR procedure to streamline the identification process.  By optimizing the multiplex PCR procedure we intend to create more consistent amplification of the loci used in MLVA analysis, which will improve …

Prevalence Of Methicillin Resistant Staphylococcus Aureus On Computer Mice On The Campus Of Southern Adventist University, Aldo J. Espinoza

Capstone Research Projects

Microorganisms are present everywhere in the environment. Unbeknownst to most people Staphylococcus aureus is a primary pathogen responsible for many skin infections and is carried by 25% of humans in the anterior nares. Due to the infective nature of Staphylococcus aureus, it is important to determine its prevalence on fomites by testing commonly used surfaces. For these reasons a research experiment was designed to determine the prevalence of S. aureus and Methicillin Resistant S. aureus (MRSA) in computer labs on the campus of Southern Adventist University during the fall semester of 2010. The research experiment was conducted in computer labs …

Epignetics And Allergic Disease, Jacob Sweeney '11

Honor Scholar Theses

In this thesis I will propose a novel framework for understanding allergy. I will argue that exposure to certain early-life environments lead to life-long allergic disease through epigenetic mechanisms. Although my argument currently exists as an unsubstantiated hypothesis, I will review indirect evidence that I have gathered throughout the immunologic and epigenetic literature to support my claim. | will then propose a set of experiments which will test my hypothesis. This thesis will address current scientific “hot topics” and their implications will challenge fundamental tenants of biology. Rather than jumping straight into the argument I will begin with some background …

Acceptance Of The Human Papillomavirus Vaccine Amount College Age Adults: Survey Research, Emily Pippo

Honors Capstones

Capstone submitted as a graduation requirement for BSU Honors Program.

Accelerating Finite State Projection Through General Purpose Graphics Processing, Thomas Trimeloni

Theses and Dissertations

The finite state projection algorithm provides modelers a new way of directly solving the chemical master equation. The algorithm utilizes the matrix exponential function, and so the algorithm’s performance suffers when it is applied to large problems. Other work has been done to reduce the size of the exponentiation through mathematical simplifications, but efficiently exponentiating a large matrix has not been explored. This work explores implementing the finite state projection algorithm on several different high-performance computing platforms as a means of efficiently calculating the matrix exponential function for large systems. This work finds that general purpose graphics processing can accelerate …

2011 April 5 - Medical Student Education Committee Minutes, Medical Student Education Committee, East Tennessee State University

Medical Student Education Committee Minutes

No abstract provided.

Inequalities Of Caries Experience In Nevada Youth Expressed By Dmft Index Vs. Significant Caries Index (Sic) Over Time, Marcia Ditmyer, Georgia Dounis, Connie Mobley, Eli Schwarz

Dental Medicine Faculty Research

Background: With the increasingly polarized distribution of dental caries among children and adolescents, the usual DMFT measure has become a less meaningful population descriptor. To re-focus on identifying the high caries prevalence group the Significant Caries Index (SiC) was created. The aims of this study were to analyze the prevalence and severity of dental caries in Nevada youth over a period of eight years and to compare its expression by means of DMFT and SiC; analyze the caries trends in the population and their underlying factors, and determine whether Nevada youth were at risk for significantly high levels of dental …

Ada News - 04/04/2011, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

April 4, 2011 Reporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

La Crosse Fish Health Center Onalaska, Wisconsin

United States Fish and Wildlife Service: Publications

The La Crosse Fish Health Center (Center) is located in Onalaska, Wisconsin in the southwest corner of the state bordering the Mississippi River. The Center is one of nine fish health laboratories operated by the U.S. Fish and Wildlife Service to serve the National Fish Hatchery System. The Center was established in 1962 and moved to its current facilities in 1995. The staff consists of eight permanent w/ 1 vacancy, 4 experience program students, and 2 student volunteers.

As an aquatic animal health laboratory, the Center services include health inspections, diagnostics, training opportunities, and technical information exchange to five Regional …

An Effective Pedagogical Approach To Understanding Qualitative And Quantitative Organic Chemistry By Using A Gas Chromatograph Mass Spectrometer, Sagir Patel

Undergraduate Student Symposium

One main objective of organic chemistry courses is to show students how much of a desired organic product is synthesized in the reaction mixture. Here, we report how a Gas Chromatography-Mass Spectrometry (GC-MS) instrument can be applied to a set of common organic chemistry experiments for identifying the main product, any minor product(s) and quantifying the product yield. GC-MS is a powerful analytical instrumentation tool capable of detecting, identifying and quantifying many volatile organic compounds. The GC-MS technique is routinely used in chemistry research and manufacturing laboratories. Despite its wide application in organic chemistry and ease of use, many colleges …

Analyzing Hotel Room Cleanliness On A Microscopic Level, Ahmad Khalid, Kodi Stevens

Undergraduate Student Symposium

The cost of a hotel room is usually correlated to the quality of the hotel room and the quality of services provided during one’s stay; e.g. the cleanliness of the room and the thoroughness of the cleaning staff. The cleaner a hotel is the more desirable it is. The goal of this experiment was to analyze hotel cleanliness at the microscopic level to determine if pathogenic micro-organisms were present. Samples were taken from telephones, toilet seats, remotes, toilet flushers, bath tubs and doorknobs from five different hotels and grown at room temperature on nutrient agar plates, counted and then isolated. …

The Effect Of Thimerosal-Containing And Thimerosal-Free Pediatric Flu Vaccine On Gene Expression In Saccharomyces Cerevisiae, Christie Rubio

Undergraduate Student Symposium

Mercury-based preservatives including thimerosal, typically added to some vaccines, may pose health concerns as mercury toxicity has been implicated in many human diseases. In this study, microarray technology and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to examine the potential effects of a typical dose of thimerosal-containing vaccine (TCV) or thimerosal-free (TFV) pediatric flu vaccine on gene expression in Saccharomyces cerevisiae, a model organism that shares roughly 30% of its genome with humans. Yeast were grown in three treatments: standard media (YEPD), YEPD plus TCV, and YEPD plus TFV. Microarray gene expression data were examined for variability both within …

The Effects Of Categorization On Memory, Logan Armstrong

Undergraduate Student Symposium

The purpose of this study was to explore the effects of categorization on our ability to remember events and objects. This is an important question because little research has tested the notion that the ability to categorize helps produce better memory. Morris & Baker-Ward (2007) studied a similar question when they examined whether having the vocabulary to describe an experience affected the ability to remember that experience. Specifically, they tested whether infants who had learned their colors prior to participating in an experiment involving different colored bubbles would recall the experiment better than those who learned the colors after the …

On Parmenides’ Reality, Nathaniel Dolan

Undergraduate Student Symposium

The treatment being submitted here for the Farquhar College of Arts and Sciences Undergraduate Student Symposium began quite simply in the form of a journal entry submitted as one of the required elements of Dr. Toscano’s Introduction to Philosophy (PHIL 1010) in the 2010 fall term. The professor’s review and critique of that original work (which was comparably brief compared to this final edition) produced remarks from Dr. Toscano that motivated me to reevaluate my own interpretations of the proposition on Reality put forth by Parmenides of Elea in the fifth century BCE. Having studied philosophy as a personal interest …

More Than Just A Memory, Sebastian Acosta-Nijamkin

Undergraduate Student Symposium

“More than just a memory” is a film about love and friendship. Ethan is confronted with a tough decision: whether to tell the girl he loves how he really feels, or whether to accept the fact that she loves someone else and that Ethan will only be a friend in her eyes. Throughout the film, Ethan is driving in a car, and flashbacks of the night where he chose to be just a friend keep entering his mind. That night he made a choice -- a choice that would change his life forever. The film is directed by Sebastian Acosta-Nijamkin.

Transcriptome Analysis Of Lagenidium Giganteum, Jennifer L. Grant

Undergraduate Student Symposium

Mosquitoes are carriers of numerous diseases for instance, malaria and west-Nile virus. Lagenidium giganteum is a parasitic organism during larval stage that infects and kills mosquitoes. In time, this could be proven to be useful in bioengineering to create an environmentally safe insecticide. Molecular research was performed in order to determine the imperative genes in Lagenidium giganteum that hinder the development in mosquito larvae. A culture of Lagenidium giganteum was used to extract RNA and initiate a transcriptome analysis. Large scale sequencing led to the annotation of 20,000 transcripts. PCR amplification was performed using a Qiagen PCR kit in order …

Cognitive Inhibition In Bilinguals Vs. Monolinguals, Juliana Acosta, Raquel Chocron, Jacqueline Pablos

Undergraduate Student Symposium

Bilingualism has been linked to enhanced cognitive abilities and these enhanced abilities are believed to be mediated by the same frontal lobe system that controls language output in bilinguals. Moreover, recent research suggests that this bilingual advantage may be moderated by degree of balance between the two languages such that those who are equally proficient in their two languages show the best performance on cognitive tasks compared to unbalanced bilinguals and monolinguals. For instance, results from one study revealed that balanced bilinguals showed significantly fewer errors on the Wisconsin Card Sorting Test (WCST) than unbalanced bilinguals. Thus, the purpose of …

Budding Brain, Kurshaad Cheenibas, Smit Patel, Adam Adbulhafid, Patrick Cao

Undergraduate Student Symposium

Mathematics is used to illuminate the world around us by describing what we see through various models. Models can range from simple linear models of gravity and line weaver Burk to complex models such as models of storms. Simple models are easy to use but lack the accuracy in prediction that more complex model provide. The difference between this dynamic model and statistical model are descriptive while dynamic model are predictive. Descriptive model make sense of data and predictive model use the data you have to make prediction. This research seeks to uncover cross disciplinary nature of dynamic model and …

A Regression Study Of The Relation Between Rocks And Fish Assemblages, Kaitlyn Brucker

Undergraduate Student Symposium

The objective of this study is to see if the amount of rock in a given transect is related to the fish that inhabited the area. A regression analysis is conducted to find the relation between the two variables, rocks and fish. Besides finding the model, the analysis includes the confidence interval for the coefficient s, residual plots, predicted intervals of the number of fish for selected values of the amount of the rock, and identifying outliers and influential points by looking at various statistics, such as RESIDUAL, STUDENTIZED RESIDUALS, COVRATIO, and DFFITS.

Adhesion Of Transferrin To Tisifilcon A Contact Lenses, Darshan Solanki, Stephanie Brinon

Undergraduate Student Symposium

Tear proteins are known to deposit differently on contact lenses of different materials, causing lens wearer comfort to be compromised. We examined the adhesion of transferrin to contact lenses made of tisifilcon A, a rigid gas-permeable silicone hydrogel (FDA Group III). Lenses were incubated 2.0 mg/ml solutions of human holo-transferrin for 1, 2, 3, and 4 days, and protein adhesion was determined by bicinchoninic acid assay. Transferrin adhesion increased from day 0 to day 3 and then dropped after days 4 and 5. This pattern resembled transferrin adhesion to lenses made of polymacon (FDA Group I), alphafilcon (FDA Group …

Tmnt: Dynamic Models Of Cancer And Hiv, Christina Gobin, Emily Nguyen, Fayssa Salomon, Arash Nasajpour

Undergraduate Student Symposium

Differential equations are used to build dynamic mathematical models for systems and nonlinear phenomena, which dynamically change with time. Ordinary differential equations describe a relation that contains functions of only one independent variable, and one or more of their derivatives with respect to that variable. Applications of those models are found in biological systems. In one study, homogeneous mathematical models are used to describe the interactions between cancerous cells and the immune system. Modeling using differential equations will allow better understanding of the behavior and spreading of those malignant cells. The models will investigate the dynamics of populations of cancer …

The Impact Of Psychological Stress On An Acute Stress Challenge: An Investigation Into Biochemical, Social And Personality Correlates, Tatiana Viena, Isabelle Barbu

Undergraduate Student Symposium

Stress exposure activates a variety of physiological coping systems. While acute stress responses are considered important for healthy coping, overexposure to stress results in an alteration of the functioning of the stress (HPA) axis, leading to a variety of deleterious psychological and physiological effects. For that reason, understanding and explaining these mechanisms is essential. Here, we sought to determine whether chronic psychological (examination) stress would result in a decreased ability to mount an acute stress and immune response. We also assessed locus of control and social support measures. Thirty subjects participated in 2 sessions (baseline and examination week) in which …

The Effects Of Traditional Weight Training Versus Crossfit On Various Measures Of Physical Performance: A Case Report In A 25 Year Old Healthy And Fit Female, Inna Dumova

Undergraduate Student Symposium

The purpose of this case study was to determine the effects of the CrossFit Workout versus Traditional Weight Training on various performance indices. During the 4-week experimental periods for each of the workouts, the subject consumed a standard Mediterranean diet. Each workout was conducted 3 times per week during those 4 weeks. No pre-workout stimulants were used in either of the workout regimens. Tests were done at the end of the experimental periods to determine the effects each workout had on strength and performance, which were compared to the pre-workout “control” data. Significant changes were found in the vertical jump …

The Effects Of Sugar Substitutes On Gene Expression In Saccharomyces Cerevisiae, Hannah Bromberg

Undergraduate Student Symposium

Sugar substitutes are marketed as being healthier than sucrose (table sugar). Within this experiment, information about how table sugar and three different sugar substitutes, Splenda (sucralose-based), Truvia (stevia-based), and Equal (aspartame-based), affect gene expression in yeast was examined. Since the genomes of yeast and humans are approximately 30% similar, the specific genes selected for analysis in yeast overlapped with genes that are expressed in humans. Thus, Saccharomyces cerevisiae (Baker’s yeast) was used as a model eukaryotic organism in order to examine the effects of sugar substitute exposure on gene expression. The yeast was grown under four different conditions: 1) A …

The Effect Of Water Temperature On Florida Manatee (Trichechus Manatus Latirostris) Abundance In Port Everglades, Brittany Lape

Undergraduate Student Symposium

Port Everglades, located near Nova Southeastern University’s Oceanographic Center, is a wintering area for the Florida manatee (Trichechus manatus latirostris), because it is a warm-water habitat. This study used data that were collected on trips through the Port, which was then examined with the following criteria for comparison: past water temperature versus current water temperature, past surveyed manatee numbers versus current manatees surveyed, and water temperature versus manatees surveyed. These comparisons were examined to illustrate any climate change trends that have occurred, as well as how those have affected the manatees in Port Everglades. These data were then …