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2010

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Evaluation Of Biological Cell Properties Using Dynamic Indentation Measurement, Guoxin Cao, Namas Chandra Jan 2010

Evaluation Of Biological Cell Properties Using Dynamic Indentation Measurement, Guoxin Cao, Namas Chandra

Department of Engineering Mechanics: Faculty Publications

Viscoelastic mechanical properties of biological cells are commonly measured using atomic force microscope (AFM) dynamic indentation with spherical tips. A semiempirical analysis based on numerical simulation is built to determine the cell mechanical properties. It is shown that the existing analysis cannot reflect the accurate values of cell elastic/dynamic modulus due to the effects of substrate, indenter tip size, and cell size. Among these factors, substrate not only increases the true contact radius but also interferes the indentation stress field, which can cause the overestimation of cell moduli. Typically, the substrate effect is much stronger than the other two influences …


Tob1 Is A Constitutively Expressed Repressor Of Liver Regeneration, Karen J. Ho, Nhue L. Do, Hasan H. Otu, Martin J. Dib, Xianghui Ren, Keiichi Enjyoji, Simon C. Robson, Ernest F. Terwilliger, Seth J. Karp Jan 2010

Tob1 Is A Constitutively Expressed Repressor Of Liver Regeneration, Karen J. Ho, Nhue L. Do, Hasan H. Otu, Martin J. Dib, Xianghui Ren, Keiichi Enjyoji, Simon C. Robson, Ernest F. Terwilliger, Seth J. Karp

Department of Electrical and Computer Engineering: Faculty Publications

How proliferative and inhibitory signals integrate to control liver regeneration remains poorly understood. A screen for antiproliferative factors repressed after liver injury identified transducer of ErbB2.1 (Tob1), a member of the PC3/BTG1 family of mito-inhibitory molecules as a target for further evaluation. Tob1 protein decreases after 2/3 hepatectomy in mice secondary to posttranscriptional mechanisms. Deletion of Tob1 increases hepatocyte proliferation and accelerates restoration of liver mass after hepatectomy. Down-regulation of Tob1 is required for normal liver regeneration, and Tob1 controls hepatocyte proliferation in a dose-dependent fashion. Tob1 associates directly with both Caf1 and cyclindependent kinase (Cdk) 1 and …


Ranching And State School Land In Cimarron County, Oklahoma, Jacqueline Vadjunec, Rebecca Sheehan Jan 2010

Ranching And State School Land In Cimarron County, Oklahoma, Jacqueline Vadjunec, Rebecca Sheehan

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Ranchers in Cimarron County, Oklahoma, have turned to leasing school trust land to sustain and sometimes expand their operations. Changes in the land tenure process have undergone profound transformations in the last 20 years, greatly impacting land use in the region. Coupled with an almost decade-long drought, land managers pursuing seemingly “traditional” agricultural practices call upon increasingly complicated, mixed private and public tenure options in order to make ends meet. Using a political ecology framework, we examine conflicting relationships between school land, the state, and local land managers as well as the sustainability of cattle ranching on school trust land …


Book Review Of Just One Vote: From Jim Walding’S Nomination To Constitutional Defeat By Ian Stewart., James A. Mcallister Jan 2010

Book Review Of Just One Vote: From Jim Walding’S Nomination To Constitutional Defeat By Ian Stewart., James A. Mcallister

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

This story is almost Shakespearean in its dramatic proportions. It includes an overly ambitious politician frustrated by his leader’s refusal to make him a cabinet minister, an even more ambitious politician’s wife pushing him beyond his abilities and fostering his bitterness, and a government leader faced with a difficult colleague whose actions brought down the government, led to the leader’s resignation, and almost destroyed a political party. Just One Vote is a welcome addition to the already significant Canadian literature on the New Democratic Party of Manitoba. The attention paid to this subject reflects the party’s democratic socialist ideology and …


Book Review Of Native Peoples And Water Rights: Irrigation, Dams, And The Law In Western Canada By Kenichi Matsui, Oliver W. Maclaren Jan 2010

Book Review Of Native Peoples And Water Rights: Irrigation, Dams, And The Law In Western Canada By Kenichi Matsui, Oliver W. Maclaren

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Native Peoples and Water Rights constitutes a valuable collection of historical case studies that shed light on a category of rights frequently overlooked. These detailed examinations identify the political, economic, and social factors of the late 19th and early 20th centuries that influenced legislative and judicial developments regarding the water rights of North America’s First Peoples. Beginning with the adoption of John Locke’s property theory in the proagrarian policies of the Jefferson administration, Matsui documents the formative period of water rights in western North America. This analysis skillfully contextualizes Chief Justice McKenna’s seminal decision in Winters v. United States 207 …


Book Review Of Identity Captured By Law: Membership In Canada’S Indigenous Peoples And Linguistic Minorities By Sébastien Grammond, Patrick Macklem Jan 2010

Book Review Of Identity Captured By Law: Membership In Canada’S Indigenous Peoples And Linguistic Minorities By Sébastien Grammond, Patrick Macklem

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

In Identity Captured by Law, Sébastien Grammond assesses the constitutional and international legality of rules that control membership in Indigenous societies and the official language minorities of Canada. Grammond’s main argument is that Indigenous and minority membership rules do not violate legal commitments to equality if there is sufficient correspondence between the legal criteria that determine membership and the actual criteria that group members themselves deploy to define themselves. Membership rules based on a racial conception of ethnic identity are less likely than those based on cultural or relational conceptions of ethnic identity to correspond to actual identities and therefore …


Book Review Of Unearthing Indian Land: Living With The Legacies Of Allotment By Kristin T. Ruppel. Tucson, Elizabeth James Jan 2010

Book Review Of Unearthing Indian Land: Living With The Legacies Of Allotment By Kristin T. Ruppel. Tucson, Elizabeth James

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

The legacies of allotment on reservations—fractionated heirship and dispossession most notably—have been apparent to numerous observers since the early 20th century. In Unearthing Indian Land: Living with the Legacies of Allotment, Kristin T. Ruppel explores contemporary efforts on the part of several Native individuals to correct more than a century of land tenure questions and outright fraud. Although such efforts have potential impact throughout Indian country, Unearthing Indian Land focuses on the local efforts of activists such as Ernee Werelus at Fort Hall in Idaho and Helen Sanders on the Quinault Reservation in Washington. Ruppel correctly traces the origins …


Book Review Of Insects Of Texas: A Practical Guide By David H. Kattes., Christopher J. Durden Jan 2010

Book Review Of Insects Of Texas: A Practical Guide By David H. Kattes., Christopher J. Durden

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

This guidebook is a well-illustrated, well-bound addition to our growing series on Texas insect fauna. Designed for the beginner and nonspecialist (and suitable for use in schools), it provides an identification aid for recognition of the groups to which common insects belong. Other references must be used in most cases to determine the species at hand. Today, BugGuide.Net will be the next step for the average reader. Most of the book is devoted to one-page presentations of a small selection of families, usually those that contain species most likely to be found by the casual observer. Common name, group name, …


Great Plains Research Volume 20, Number 2 (Fall 2010): Cover And Contents Jan 2010

Great Plains Research Volume 20, Number 2 (Fall 2010): Cover And Contents

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

SOCIAL SCIENCES

Ranching and State School Land in Cimarron County, Oklahoma Jacqueline Vadjunec and Rebecca Sheehan. 163

Determinants of Net Migration in Montana Evelyn D. Ravuri. 179

A Test of Personal Characteristcs that Influence Farmers’ Pro-Environmental Behaviors Courtney E. Quinn and Mark E. Burbach.193

GIS Spatial Analysis of University of Nebraska at Kearney Alumni Cohorts, 1930–2004 Paul R. Burger and Brett R. Chloupek. 205

The Really Good Buffalo Concept Test for “Values Added” Bison Diane Rickerl, Tim Nichols, and Carol Cumber. 215

NATURAL SCIENCES

Livestock Responses to Complementary Forages in Shortgrass Steppe Justin D. Derner and Richard H. Hart. 223 …


This Is Not Your Father's Thor: Using Comics To Make Mythology Meaningful, Nick Kremer Jan 2010

This Is Not Your Father's Thor: Using Comics To Make Mythology Meaningful, Nick Kremer

SANE journal: Sequential Art Narrative in Education

This article documents one teacher's attempts to use Contact Zone pedagogy and contemporary comics to improve his instruction of classical mythology in a high school Language Arts classroom. He found that students were much more interested in and able to critically discuss myths from other cultures when they first had "model" conversations about myths from their own current society. The author makes the case for comic books as the most viable source of contemporary American mythology, reviews three specific graphic novels [The Dark Knight Returns, Marvels, and Ultimate Spider-Man] that work well in a mythology unit, discusses specific lessons and …


Everything I Learned About Teaching In The Contact Zone I Learned From Charles Xavier And The Uncanny X-Men, James Bucky Carter Ph.D. Jan 2010

Everything I Learned About Teaching In The Contact Zone I Learned From Charles Xavier And The Uncanny X-Men, James Bucky Carter Ph.D.

SANE journal: Sequential Art Narrative in Education

The article asserts that reading and studying comics via the lens of the contact zone help illuminate comics' literary and literacy value and helps illuminate notions of comics art and notions of schooling.


Effects Of Splat® Gm Sprayable Pheromone Formulation On Gypsy Moth Mating Success, Ksenia S. Onufrieva, Kevin W. Thorpe, Andrea D. Hickman, Patrick C. Tobin, Donna S. Leonard, E. Anderson Roberts Jan 2010

Effects Of Splat® Gm Sprayable Pheromone Formulation On Gypsy Moth Mating Success, Ksenia S. Onufrieva, Kevin W. Thorpe, Andrea D. Hickman, Patrick C. Tobin, Donna S. Leonard, E. Anderson Roberts

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Several integrated pest management programs rely on the use of mating disruption tactics to control insect pests. Some programs specifically target non-native species, such as the gypsy moth, Lymantria dispar (L.) (Lepidoptera: Lymantriidae).We evaluated SPLAT ® GM, a new sprayable formulation of the gypsy moth sex pheromone disparlure, for its ability to disrupt gypsy moth mating. The study was conducted in 2006, 2007, and 2008 in forested areas in Virginia, USA. Mating success of gypsy moth females was reduced by >99% and male moth catches in pheromone-baited traps by >90%, in plots treated with SPLAT ® GM at dosages ranging …


Differentiating Instruction In The High School Science Classroom: Instructional Accommodations For Students With Attention-Deficit/Hyperactivity Disorder, English Language Learners, And Gifted Learners, Nicole M. Kesselring Jan 2010

Differentiating Instruction In The High School Science Classroom: Instructional Accommodations For Students With Attention-Deficit/Hyperactivity Disorder, English Language Learners, And Gifted Learners, Nicole M. Kesselring

Graduate Research Papers

Students in today's classrooms come from many different backgrounds and have many different educational needs and learning styles. Today's education emphasizes inclusion of students with learning disabilities and a reduction of out-of-class services for gifted learners. Working to meet the needs of the broad spectrum of students is a big challenge for many of today's science teachers. Indications are that most teachers are making few proactive modifications based on learner variance. Inclusion of students with Attention-Deficit/Hyperactivity Disorder (ADHD) and English Language Learners (ELL) in science classrooms along with gifted learners calls for a need of differentiated or academically responsive instruction. …


“We Just Don’T Have The Possibility Yet”: U.S. Latina/O Narratives On Study Abroad, Kevin R. Mcclure, Katalin Szelényi, Elizabeth Niehaus, Aeriel A. Anderson, Jeffrey Reed Jan 2010

“We Just Don’T Have The Possibility Yet”: U.S. Latina/O Narratives On Study Abroad, Kevin R. Mcclure, Katalin Szelényi, Elizabeth Niehaus, Aeriel A. Anderson, Jeffrey Reed

Department of Educational Administration: Faculty Publications

Whether indirectly from governmental and non-governmental organizations or directly from higher education institutions, students receive messages that they should study abroad. Studying in a foreign country is considered essential if students are to be marketable to future employers and prepared to lead the U.S. into a new era. Despite the presence of such messages, the understanding of what it means to be absent from the undergraduate student population willing and able to study in a foreign country is severely limited. Importantly, what are the perceptions and experiences of students who repeatedly hear the value of study abroad and who, at …


A Model Of Tuberculosis Transmission And Intervention Strategies In An Urban Residential Area, Elsje Pienaar, Aaron M. Fluitt, Scott E. Whitney, Alison G. Freifeld, Hendrik J. Viljoen Jan 2010

A Model Of Tuberculosis Transmission And Intervention Strategies In An Urban Residential Area, Elsje Pienaar, Aaron M. Fluitt, Scott E. Whitney, Alison G. Freifeld, Hendrik J. Viljoen

Department of Chemical and Biomolecular Engineering: Faculty Publications

The model herein aims to explore the dynamics of the spread of tuberculosis (TB) in an informal settlement or township. The population is divided into households of various sizes and also based on commuting status. The model dynamics distinguishes between three distinct social patterns: the exposure of commuters during travel, random diurnal interaction and familial exposure at night. Following the general SLIR models, the population is further segmented into susceptible (S), exposed/latently infected (L), active/infectious (I), and recovered (R) individuals. During the daytime, commuters travel on public transport, while non-commuters randomly interact in the community to mimic chance encounters with …


Corrosion Of Steel Shipwrecks In The Marine Environment: Uss Arizona—Part 2, Donald L. Johnson, Brent M. Wilson, James D. Carr, Matthew A. Russell, Larry E. Murphy, David L. Conlin Jan 2010

Corrosion Of Steel Shipwrecks In The Marine Environment: Uss Arizona—Part 2, Donald L. Johnson, Brent M. Wilson, James D. Carr, Matthew A. Russell, Larry E. Murphy, David L. Conlin

Department of Chemistry: Faculty Publications

This article is a continuation of Part 1 (October 2006 MP) on the USS Arizona hull. It discusses additional methods being investigated for use in determining corrosion rates with a minimal impact on the existing structure. Marine biofouling (concretion) has accumulated on the hull. Concretion samples have been studied using x-ray diffraction, environmental electron microscopy, and corrosion potential (Ecorr) to characterize the relationship between concretion and the steel substrate. The data presented in this study confirm the viability of concretion analysis as a minimum-impact procedure to estimate the corrosion rate of mild steel in seawater.


Response To Akhil Reed Amar's Address On Applications And Implications Of The Twenty-Fifth Amendment, John D. Feerick Jan 2010

Response To Akhil Reed Amar's Address On Applications And Implications Of The Twenty-Fifth Amendment, John D. Feerick

Faculty Scholarship

Life has taught those of us who have lived as long as I have that the seemingly impossible can happen and that we must be prepared to deal with the unimaginable on a moment's notice. In October 1963, I wrote an article for the Fordham Law Review in which I contemplated the need for such preparations should the unimaginable indeed strike: "The problem of presidential inability has now been generally forgotten by our national legislators as well as by the public. Since we have a young, able and healthy President, all indications are that the issue will remain dormant until …


Review Of Breathing In The Fullness Of Time By William Kloefkorn, David Pichaske Jan 2010

Review Of Breathing In The Fullness Of Time By William Kloefkorn, David Pichaske

Great Plains Quarterly (through 2013)

The central metaphor in this final installment of Nebraska State Poet Bill Kloefkorn's four-part celebration of life in the Great Plains is air. Whereas his three previous memoirs- water, fire, and earth-explored childhood and adolescent memories, Kloefkorn here focuses mainly on adult experiences in college and the Marine Corps, teaching English at Nebraska Wesleyan, classroom adventures as a poet-in-residence, and his celebrated victory in the North Platte, Nebraska, hog-calling contest. Time and tradition are central concerns in this book, as is desire-in football and marriage, in writing poetry and being a good Marine or hog caller, in overcoming adversities like …


The Costs Of Consensus In Statutory Construction, Ethan J. Leib, Michael Serota Jan 2010

The Costs Of Consensus In Statutory Construction, Ethan J. Leib, Michael Serota

Faculty Scholarship

Finding methodological consensus for statutory interpretation cases is all the rage these days.1 Some in the academy sing the praises of a singular judicial approach to questions of statutory interpretation and bemoan the frustrations associated with judges implementing a mélange of interpretive techniques. And now, thanks to Abbe Gluck’s authoritative article, Laboratories of Statutory Interpretation, proponents of interpretive uniformity have evidence that some state courts seem to be applying methodological stare decisis to decide questions of statutory interpretation. After exhaustive reading and analysis of state statutory interpretation cases—cases that have received far less attention than their federal counterparts—Gluck describes several …


Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff Jan 2010

Prolonged Intensive Dominance Behavior Between Gray Wolves, Canis Lupus, L. David Mech, H. Dean Cluff

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Dominance is one of the most pervasive and important behaviors among wolves in a pack, yet its significance in free-ranging packs has been little studied. Insights into a behavior can often be gained by examining unusual examples of it. In the High Arctic near Eureka, Nunavut, Canada, we videotaped and described an unusually prolonged and intensive behavioral bout between an adult male Gray Wolf (Canis lupus) and a male member of his pack, thought to be a maturing son.With tail raised, the adult approached a male pack mate about 50 m from us and pinned and straddled this …


Native Daughters, Christina Devries, Jordan Pascale, Shannon Smith, Katie Stearns, Carson Vaughan, Molly Young, Clay Lomneth, Alan Eno, Astrid Munn, Matt Buxton, Judi M. Gaiashkibos, Shannon Smith, Hannah Peterson, Elizabeth Gamez, Maggie Liggett, Katie Stearns, Natasha Richardson, Johnna Hjersman, Krista Vogel, Elizabeth Gasaway, Charlie Pfister, Matt Buxton, Anthony Troester, Christine Lesiak, Scott Winter, John Wunder, Joe Starita, Princella Parker, Nancy Kelsey Jan 2010

Native Daughters, Christina Devries, Jordan Pascale, Shannon Smith, Katie Stearns, Carson Vaughan, Molly Young, Clay Lomneth, Alan Eno, Astrid Munn, Matt Buxton, Judi M. Gaiashkibos, Shannon Smith, Hannah Peterson, Elizabeth Gamez, Maggie Liggett, Katie Stearns, Natasha Richardson, Johnna Hjersman, Krista Vogel, Elizabeth Gasaway, Charlie Pfister, Matt Buxton, Anthony Troester, Christine Lesiak, Scott Winter, John Wunder, Joe Starita, Princella Parker, Nancy Kelsey

College of Journalism and Mass Communications: Student Media

For almost two years, Nebraska’s College of Journalism and Mass Communications has benefited from this perfect storm, riding a project wave dedicated to a singular idea: You can’t really understand American history without understanding Native American history. And you can’t understand Native American history without understanding the critical role Native women have played in defining, enriching and protecting that history. Underwritten by a $125,000 Carnegie Foundation grant, this journalism project is intended to substantially raise the profile of Native Daughters. To that end, the college enrolled two dozen of its best and brightest students – reporters, photographers, videographers, Web masters, …


Limiting Factors Of Five Rare Plant Species In Mesic Forests, Hawai'i Volcanoes National Park, Linda W. Pratt, Joshua R. Vandemark, Melody Euaparadorn Jan 2010

Limiting Factors Of Five Rare Plant Species In Mesic Forests, Hawai'i Volcanoes National Park, Linda W. Pratt, Joshua R. Vandemark, Melody Euaparadorn

United States Geological Survey: Staff Publications

Five rare or endangered plant species native to Kīpuka Puaulu and Kīpuka Kī were studied for two years to determine their stand structure, patterns of reproductive phenology, success of fruit production, potential pollinators, greenhouse seed germination rates, presence of soil seed banks, impacts of seed-predating rats, seed predation by insects, seedling predation by Kalij pheasant (Lophura leucomelanos), and seedling survival with different treatments. Species monitored were the trees Hibiscadelphus giffardianus (hau kuahiwi), Melicope hawaiensis (manena), M. zahlbruckneri (alani), and Zanthoxylum dipetalum var. dipetalum (kāwa`u), and the vine Sicyos macrophyllus (`ānunu).

Limiting factors identified for H. giffardianus were rat …


Status Of The California Condor (Gymnogyps Californianus) And Efforts To Achieve Its Recovery, Jeffrey R. Walters, Scott R. Derrickson, D. Michael Fry, Susan M. Haig, John M. Marzluff, Joseph M. Wunderle Jr. Jan 2010

Status Of The California Condor (Gymnogyps Californianus) And Efforts To Achieve Its Recovery, Jeffrey R. Walters, Scott R. Derrickson, D. Michael Fry, Susan M. Haig, John M. Marzluff, Joseph M. Wunderle Jr.

United States Geological Survey: Staff Publications

The California Condor (Gymnogyps californianus; hereafter “condor”; Fig. 1) has long been symbolic of avian conservation in the United States. Its large size, inquisitiveness, and association with remote places make it highly charismatic, and its decline to the brink of extinction aroused a continuing public interest in its plight. By 1982, only 22 individuals remained of this species whose range once encompassed much of North America. The last wild bird was trapped and brought into captivity in 1987, which rendered the species extinct in the wild (Snyder and Snyder 1989). In the 1980s, some questioned whether viable populations …


Avian Subspecies And The U.S. Endangered Species Act, Susan M. Haig, Jesse D'Elia Jan 2010

Avian Subspecies And The U.S. Endangered Species Act, Susan M. Haig, Jesse D'Elia

United States Geological Survey: Staff Publications

Scientific debate over identification of taxa below the species level has persisted for centuries. This issue can be especially problematic for avian species, because dispersal is often orders of magnitude greater than in other vertebrates, leaving genetic differences among groups proportionately smaller. While the debate lingers, management decisions, often with millions of dollars and potential extinctions resting on the outcome, are regularly made by agencies tasked with maintaining lists of threatened and endangered taxa. With outdated taxonomic treatments and no formal policy or guidelines for defining species or subspecies, agencies have no authority to cite in determining limits to species …


Court Review, Volume 46, Issue 4 (Complete) Jan 2010

Court Review, Volume 46, Issue 4 (Complete)

Court Review: Journal of the American Judges Association

Book Review

116 Fugitive Justice: Slavery and the Law in Pre-Civil War America (Karen Arnold-Burger)

Articles

118 Iowa’s 2010 Judicial Election: Appropriate Accountability or Rampant Passion? (Roy A. Schotland)

130 Breaking Impasses in Judicial Settlement Conferences: Seven (More) Techniques for Resolution (Morton Denlow)

136 Selected Cases from the United States Supreme Court’s 2009-2010 Term (Tim J. Davis)

Departments

114 Editor’s Note

115 President’s Column

152 The Resource Page


Iowa’S 2010 Judicial Election: Appropriate Accountability Or Rampant Passion?, Roy A. Schotland Jan 2010

Iowa’S 2010 Judicial Election: Appropriate Accountability Or Rampant Passion?, Roy A. Schotland

Court Review: Journal of the American Judges Association

The 2010 Iowa judicial elections were, as former Arkansas Governor Mike Huckabee said soon after, of an “historic nature,” likely “one that . . . will give legs to a larger movement over the next few years.” The election he referred to, in which Iowans voted against retention of three justices who had participated in their Supreme Court’s unanimous decision to uphold gay marriage, surely is one of the most significant judicial elections ever. It also was the highest-visibility judicial election since 1986, when Californians voted down the retention of Chief Justice Rose Bird and two of her colleagues.

Even …


Induction Of Interferon And Interferon Signaling Pathways By Replication Of Defective Interfering Particle Rna In Cells Constitutively Expressing Vesicular Stomatitis Virus Replication Proteins, Debasis Panda, Phat X. Dinh, Lalit Beura, Asit K. Pattnaik Jan 2010

Induction Of Interferon And Interferon Signaling Pathways By Replication Of Defective Interfering Particle Rna In Cells Constitutively Expressing Vesicular Stomatitis Virus Replication Proteins, Debasis Panda, Phat X. Dinh, Lalit Beura, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

We show here that replication of defective interfering (DI) particle RNA in HEK293 cells stably expressing vesicular stomatitis virus (VSV) replication proteins potently activates interferon (IFN) and IFN signaling pathways through upregulation of IFN- promoter, IFN-stimulated response element (ISRE) promoter, and NF-κB promoter activities. Replication of DI particle RNA, not mere expression of the viral replication proteins, was found to be critical for induction of IFN and IFN signaling. The stable cells supporting replication of DI RNA described in this report will be useful in further examining the innate immune signaling pathways and the host cell functions in viral genome …


Chronology And Evolution Of The Hiv-1 Subtype C Epidemic In Ethiopia, Damien C. Tully, Charles Wood Jan 2010

Chronology And Evolution Of The Hiv-1 Subtype C Epidemic In Ethiopia, Damien C. Tully, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective—To reconstruct the onset date and evolutionary history of the HIV-1 subtype C epidemic in Ethiopia - one of the earliest recorded subtype C epidemics in the world.

Design—HIV-1 C env sequences with a known sampling year isolated from HIV-1 positive patients from Ethiopia between 1984 and 2003.

Methods—Evolutionary parameters including origin and demographic growth patterns were estimated using a Bayesian coalescent-based approach under either strict or relaxed molecular clock models.

Results—Bayesian evolutionary analysis indicated a most recent common ancestor date of 1965 with three distinct epidemic growth phases. Regression analysis of root-to-tip distances revealed a highly similar estimate for …


Enhancement Of Autophagy During Lytic Replication By The Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator, Hui-Ju Wen, Zhilong Yang, You Zhou, Charles Wood Jan 2010

Enhancement Of Autophagy During Lytic Replication By The Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator, Hui-Ju Wen, Zhilong Yang, You Zhou, Charles Wood

Nebraska Center for Virology: Faculty Publications

Autophagy is one of two major degradation systems in eukaryotic cells. The degradation mechanism of autophagy is required to maintain the balance between the biosynthetic and catabolic processes and also contributes to defense against invading pathogens. Recent studies suggest that a number of viruses can evade or subvert the host cell autophagic pathway to enhance their own replication. Here, we investigated the effect of autophagy on the KSHV (Kaposi’s sarcoma-associated herpesvirus) life cycle. We found that the inhibition of autophagy reduces KSHV lytic reactivation from latency, and an enhancement of autophagy can be detected during KSHV lytic replication. In addition, …


Heat Flow And Hydrologic Characteristics At The And-1b Borehole, Andrill Mcmurdo Ice Shelf Project, Antarctica, Roger H. Morin, Trevor Williams, Stuart A. Henrys, Diana Magens, Frank Niessen, Dhiresh Hansaraj Jan 2010

Heat Flow And Hydrologic Characteristics At The And-1b Borehole, Andrill Mcmurdo Ice Shelf Project, Antarctica, Roger H. Morin, Trevor Williams, Stuart A. Henrys, Diana Magens, Frank Niessen, Dhiresh Hansaraj

United States Geological Survey: Staff Publications

The Antarctic Drilling Program (ANDRILL) successfully drilled and cored a borehole, AND-1B, beneath the McMurdo Ice Shelf and into a flexural moat basin that surrounds Ross Island. Total drilling depth reached 1285 m below sea floor (mbsf) with 98 percent core recovery for the detailed study of glacier dynamics. With the goal of obtaining complementary information regarding heat fl ow and permeability, which is vital to understanding the nature of marine hydrogeologic systems, a succession of three temperature logs was recorded over a five day span to monitor the gradual thermal recovery toward equilibrium conditions. These data were extrapolated to …