Digital Commons Network^™

The Use Of Preclinical Models To Improve The Treatment Of Retinoblastoma, Katie Marie Nemeth

Theses and Dissertations (ETD)

Rodent models play an essential role in the development of new chemotherapeutics and dosing regimes. It is often difficult to carryout a clinical study for pediatric cancers due to the small patient population. Retinoblastoma, a pediatric cancer of the eye, is one example of a pediatric cancer that can benefit from preclinical studies. Over the years various retinoblastoma rodent models have been developed used to test various combination of broad-spectrum systemic chemotherapy. It was found form these studies that the combination of topotecan and carboplatin was effective. However both drugs cause myelosuppression and therefore administrating both of these drugs systemically …

Preosteoblast Behavior On A Synthetic Calcium Phosphate Scaffold Coated With Cell Derived Matrix, Daniel A. Young

Theses and Dissertations (ETD)

Bone grafts are commonly used for the treatment of large bone defects. Biodegradable, synthetic bone grafts combined with cells and growth factors as a tissue engineered construct can provide benefits over the common autografts and allografts currently used. Calcium phosphate (CaP) materials offer high biocompatibility and chemical similarity to natural bone tissue. Biodegradable beta-tricalcium phosphate (β-TCP) scaffolds with an interconnected porous network have been created to promote tissue ingrowth and regeneration in vitro and in vivo. Extracellular matrix (ECM) coated on titanium and polymer scaffolds has been shown to improve cell response to those material surfaces. Here, we attempted …

Discovery Of Quinic Acid Derivatives As Oral Anti-Inflammatory Agents, Kui Zeng

Theses and Dissertations (ETD)

Quinic acid (QA) esters found in hot water extracts of Uncaria tomentosa (a.k.a. Cat’s claw) exert anti-inflammatory activity through mechanisms involving inhibition of the pro-inflammatory transcription factor nuclear factor kappa B (NF-κB). Herein, we described the synthesis and biological testing of novel QA derivatives. Inhibition of NF-κB was assessed using A549 (Type II alveolar epithelial-like) cells that stably express a secreted alkaline phosphatase (SEAP) reporter driven by an NF-κB response element. A549- NF-κB cells were stimulated with TNF-α (10 ng/mL) in the presence or absence of QA derivative for 18 hours followed by measurement of SEAP activity. Amide substitution at …

The Effects Of Betadine®, Polyvinylpyrrolidone (Pvp), And Iodine On Regeneration In The Planarian Dugesia Tigrina., Traci R. Shaffer

Electronic Theses and Dissertations

The freshwater planarian has a great capacity to regenerate and is an ideal animal model in the study of stem cell and regeneration biology. In this study planarian regenerating new tails were exposed to nonlethal doses of Betadine®, Polyvinylpyrrolidone (PVP), and Iodine. Betadine® is a topical antiseptic commonly used in the healthcare setting and may have a detrimental effect on wound healing. PVP is linked to iodine to create povidone-iodine, the active ingredient in Betadine®. Initially, a preliminary test was performed on the worms to determine the nonlethal concentrations of these chemicals . After this nonlethal concentration was determined, tails …

Optimization And Economic Evaluation Of Industrial Gas Production And Combined Heat And Power Generation From Gasification Of Corn Stover And Distillers Grains, Ajay Kumar, Yaşar Demirel, David D. Jones, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thermochemical gasification is one of the most promising technologies for converting biomass into power, fuels and chemicals. The objectives of this study were to maximize the net energy efficiency for biomass gasification, and to estimate the cost of producing industrial gas and combined heat and power (CHP) at a feedrate of 2,000 kg/h. Aspen Plusbased model for gasification was combined with a CHP generation model, and optimized using corn stover and dried distillers grains with solubles (DDGS) as the biomass feedstocks. The cold gas efficiencies for gas production were 57% and 52%, respectively, for corn stover and DDGS. The selling …

Glycopeptidome Of A Heavily N-Glycosylated Cell Surface Glycoprotein Of Dictyostelium Implicated In Cell Adhesion, Christa L. Feasley, Jennifer M. Johnson, Christopher M. West, Catherine P. Chia

School of Biological Sciences: Faculty Publications

Genetic analysis has implicated the cell surface glycoprotein gp130 in cell interactions of the social amoeba Dictyostelium, and information about the utilization of the 18 N-glycosylation sequons present in gp130 is needed to identify critical molecular determinants of its activity. Various glycomics strategies, including mass spectrometry of native and derivatized glycans, monosaccharide analysis, exoglycosidase digestion, and antibody binding, were applied to characterize a nonanchored version secreted from Dictyostelium. s-gp130 is modified by a predominant Man8GlcNAc4 species containing bisecting and intersecting GlcNAc residues and additional high-mannose N-glycans substituted with sulfate, methyl-phosphate, and/or core R3-fucose. Site mapping confirmed the occupancy …

Lineage-Specific Patterns Of Functional Diversification In The Α- And Β-Globin Gene Families Of Tetrapod Vertebrates, Federico G. Hoffmann, Jay F. Storz, Thomas A. Gorr, Juan C. Opazo

Jay F. Storz Publications

The α- and β-globin gene families of jawed vertebrates have diversified with respect to both gene function and the developmental timing of gene expression. Phylogenetic reconstructions of globin gene family evolution have provided suggestive evidence that the developmental regulation of hemoglobin synthesis has evolved independently in multiple vertebrate lineages. For example, the embryonic β-like globin genes of birds and placental mammals are not 1:1 orthologs. Despite the similarity in developmental expression profiles, the genes are independently derived from lineage-specific duplications of a β-globin pro-ortholog. This suggests the possibility that other vertebrate taxa may also possess distinct repertoires of globin genes …

Coenzyme Q10 And Oxidative Stress Markers In Seminal Plasma Of Iraqi Patients With Male Infertility, Omar F. Abdul-Rasheed, Yahya Y. Farid, Ussama S. Al-Nasiri

Saudi Medical Journal

OBJECTIVE: To evaluate seminal plasma coenzyme Q10 (CoQ10) levels and oxidative stress in patients with different types of male infertility. METHODS: A case-control study was carried out in the Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq from the period of November 2004 to July 2006. Sixty patients with male infertility were recruited from Al-Kadhimia Teaching Hospital, Baghdad and included in this study. The male patients were categorized according to their seminal fluid parameters to oligozoospermia (n=32), azoospermia (n=22), and asthenozoospermia (n=6). All obtained results from infertile men groups were compared with age-matched healthy volunteers as …

Proteomic Analysis Of Clear Cell Renal Cell Carcinoma. Identification Of Potential Tumor Markers, Chuan Y. Sun, Ya C. Zang, Yu X. San, Wei Sun, Liang Zhang

Saudi Medical Journal

OBJECTIVE: To identify new tumor specific proteins of renal cell carcinoma (RCC) using proteomic analysis. METHODS: Nine renal cell carcinomas were resected and these surgical operations were carried out from June 2007 to September 2008 in the Urology Department of the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. The cancer tissues and para-cancer normal tissues were preserved in liquid nitrogen. We analyzed the RCC tissues and para-cancer normal tissues by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The 16 differentially expressed protein spots (p<0.05 by Student t-test) were identified by matrix assisted laser desorption ionization/time of flight mass spectrometry and tandem mass spectrometry. RESULTS: Six proteins were down regulated and 10 proteins were up regulated in clear cell RCC compared with corresponding normal kidney tissue. The down regulated proteins were calbindin, ester hydrolase C11orf54, alcohol dehydrogenase, ammecr1-like protein, adenosine diphosphate (ADP)/adenosine-59-triphosphate (ATP) translocase 3 and leucine-tyrosine-arginine (LYR) motif-containing protein 5. The up regulated proteins were hypoxia inducible domain family member 1A, glutathione S-transferase P, thioredoxin-dependent peroxide reductase, peroxiredoxin-6, CD2-associated protein, annexin A5, gamma-enolase, retinal dehydrogenase 1, vimentin, and protein-glutamine gamma-glutamyltransferase 2. CONCLUSION: The data provide potential tumor markers for diagnosis of RCC.

Therapeutic Outcomes Of Older Patients With Acute Myeloid Leukemia, Raihan Sajid, Bushra Moiz, Natasha Ali, Nausheen Kamran, Adil N. Adil, Usman Shaikh, Mohammad Khurshid

Saudi Medical Journal

OBJECTIVE: To evaluate the therapeutic outcomes of acute myeloid leukemia (AML) in elderly patients. METHODS: This study was conducted at the Aga Khan University Hospital, Karachi, Pakistan over 11 years from January 1997 to August 2008. This was a descriptive case series study. We investigated the impact of disease biology and various treatment protocols on the outcome in this population. RESULTS: A total of 55 evaluable patients (>60 years of age) were diagnosed with AML including 34 (61.8%) males and 21 (38.2%) females. The median age was 67 years (range 60-86 years) at the time of presentation. The AML …

Regional Disparity In Prevalence Of Malnutrition In Saudi Children, Mohammad I. El-Mouzan, Abdullah S. Al-Herbish, Abdullah A. Al-Salloum, Peter J. Foster, Ahmad A. Al-Omar, Mansour M. Qurachi, Tatjana Kecojevic

Saudi Medical Journal

OBJECTIVE: To evaluate the regional difference in the prevalence of malnutrition in Saudi children. METHODS: Data for this study were collected over 2 years (2004 and 2005). A cross-sectional representative sample of the Saudi population of healthy children below 5 years of age was used to calculate the prevalence of malnutrition. The study was carried out in the College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia. Body measurements of the weight, length, and height were performed according to standard recommendations. Standard deviation scores were determined using the Lambda, Mu, and Sigma (LMS) statistical methodology. The 1978 NCHS/WHO …

The Ability Of The Pre-Admission Criteria To Predict Performance In A Saudi Medical School, Mohammad O. Al-Rukban, Fadi M. Munshi, Hamza M. Abdulghani, Ibrahim Al-Hoqail

Saudi Medical Journal

OBJECTIVE: To evaluate the ability of preadmission criteria used in most health professional schools in Saudi Arabia to predict the in-program performance. METHODS: This retrospective cohort study was conducted at King Fahd Medical City, Faculty of Medicine, Riyadh, Kingdom of Saudi Arabia between July and September 2008. Four sets were used to examine the predictive power of preadmission variables. The variables are the academic abilities (high school grades), aptitude test, achievement test, and an interview. The criterion variables were the undergraduate grade point averages9 (GPAs) of medical college students (n=193). The correlation between admission variables and the GPA was examined …

Nucleophosmin Gene Mutation In Acute Myeloid Leukemia Patients, Mervat M. Khorshied, Wael A. Said, Hebat A. Shaaban

Saudi Medical Journal

No abstract provided.

Comparison Of Extraction Techniques For Δ-9-Tetrahydrocannabinol From Urine, Ashlyn Christine Harmon

Master's Theses

With the increasing use of marijuana, efficient methods to extract and detect Δ-9-THC in urine samples and relate the concentrations found to time is a necessity so that impairment can be determined. Δ-9-THC is conjugated to glucuronic acid in urine and must be freed before the compound can be detected by chromatographic procedures. In past research, either a strong alkaline solution or β-glucuronidase has been used to cleave the bond between the Δ-9-THC and glucuronic acid before an alkaline extraction. Δ-9-THC has a pKa of 10.6 and therefore is always extracted at an alkaline pH. In this study, both a …

Variation In Social Behavior Throughout The Estrous Cycle Of A Captive Killer Whale Orcinus Orca, Kristina Marie Horback

Master's Theses

The study of cetaceans in captivity provides information on behavior, acoustics, reproduction and physiology that is often difficult to obtain with free-ranging populations. The present study examined the influence of the estrous cycle of a captive female killer whale on the rate, duration, initiation and reception of social behavior she performed with her only pool mate, a male Atlantic bottlenose dolphin. Although these two delphinid species do not commonly associate in the wild, these pool mates often engaged in affiliative tactile and social behaviors. The objectives of this project were to: (a) examine the influence of cycli~ gonadal steroid hormones …

Avoiding Confirmation Bias Through Awareness And Training, Jon Stacy Byrd

Master's Theses

In any profession where conclusions or opinions are generated by humans, there is a realization of possible error. In the profession of forensic science, errors are not taken lightly. In fact most agencies have been known to have had a no tolerance policy when it comes to errors. This means that if an analyst made a mistake in casework, he or she could be terminated. Over the past 15 years, the words "error" and "bias" have become synonymous with forensic science in the media. Major errors have surfaced in several high profile cases only to cast doubt on the forensic …

Comparison Of The Biological Attributes, First And Second Level Of Detail Of Friction Ridge Skin Of The Palms And Fingers, Kelley Bryant Counts

Master's Theses

Since the case of Daubert v. Merrell Dow Pharmaceuticals, the Daubert criteria is nothing new to forensic science. Today's practices and techniques presented in a court of law are held to high scientific scrutiny. For nearly 100 years, expert witness testimony concerning fingerprint identification has been allowed into courts with little challenge, as it is supported by several areas of research that acknowledge that no two fingerprints are the same; they will remain unchanged during an individuals lifetime, and that fingerprints have a general systematic classification system. In the past, the assumption was always made that palm prints adhered to …

A Parameterization Study Of Short Read Assembly Using The Velvet Assembler, Alex Christopher Elliot

Master's Theses

In this study, we examine approaches to the problem of assembling large, contiguous sections of genetic code from short reads generated from laboratory techniques. We explore the Eulerian Path approach in detail, utilizing a de Bruijn Graph, and demonstrate current software technologies and algorithms using a sample genome. We investigate the input parameters of Velvet and discuss choice implications in the context of the E. coli putA/b1014 gene.

The Development Of A Comprehensive Mechanism For Intracellular Calcium Oscillations: A Theoretical Approach And An Experimental Validation, Amanda A. Borges, Deanna Salter, Sandor Kadar, Steven B. Symington

Pell Scholars and Senior Theses

Calcium is an important second messenger for cellular communication. Theoretical models help scientists understand its signalling mechanism. A comprehensive model was developed in order to minimize any limitations in the models currently presented in the literature. Experimental results support the model and therefore the theoretical model provides a plausible explanation of the dynamics of the calcium-signaling mechanism. In the future, additional verification will be performed using various experimental configurations on PC12 cells. Further, the model will be used to predict the response of cells to environmental factors such as pesticides and heavy metals.

Screening Culture: Is Western Media To Blame For “Tainting” Traditional Third World Values?, Angelina M. Campbell

Pell Scholars and Senior Theses

This thesis questions the idea that, through globalization, American media infiltrates Third World countries and subsequently changes or "taints" traditional culture.

The Influence Of Cardiorespiratory Fitness On Lung Cancer Mortality, Xuemei Sui, Duck-Chul Lee, Charles E. Matthews, Swann Arp Adams, James R. Hébert, Timothy S. Church, Chong-Do Lee, Steven N. Blair

Faculty Publications

Purpose—Previous studies have suggested that higher levels of physical activity may lower lung cancer risk; however, few prospective studies have evaluated lung cancer mortality in relation to cardiorespiratory fitness (CRF), an objective marker of recent physical activity habits. Methods—Thirty-eight thousand men, aged 20 to 84 years without history of cancer, received a preventive medical examination at the Cooper Clinic in Dallas, TX, between 1974 and 2002. CRF was quantified as maximal treadmill exercise test duration and was grouped for analysis as low (lowest 20% of exercise duration), moderate (middle 40%), and high (upper 40%). Results—A total of 232 lung cancer …

Il-2 Regulation Of Il-24 Expression Leads To Growth Suppression Of Melanoma Cells, Emily Jen

Dissertations and Theses (Open Access)

Melanoma is known to be highly resistant to chemotherapy. Treatment with high dose IL-2 has shown significant clinical benefit in a minority of metastatic melanoma patients and has lead to long term survival in a few cases. However, this treatment is associated with excessive multiorgan toxicities, which severely limits its use. We hypothesize that one mechanism of effective IL-2 therapy is through the direct upregulation of IL-24 production in melanoma tumors and subsequent IL-24 mediated tumor growth suppression.

Five melanoma cell lines were treated with high dose recombinant hIL-2 at 1000U/ml. Three of the cell lines (A375, WM1341, WM793) showed …

Impact Of Workplace Stress On Job Satisfaction Moderated By Religious Coping, Celina A. Esparza

Theses and Dissertations - UTB/UTPA

Workplace stress has been associated with negative job satisfaction outcomes, therefore leading employees to engage in coping strategies to minimize the consequences of the stressors. Although much research has been conducted on workplace stressors and their consequences, a small amount of literature exists on how women and Hispanics cope with workplace stressors or how people cope with the stressors in religious ways. To investigate the relationships among these factors, data was collected via an online survey completed by 268 university employees at a southwestern university. Self-report data collected suggested that both women and Hispanics use religion to cope with workplace …

Differential Use Of Language By Adolescents Across Modes Of Written Communication, Kris A. Vossler

Theses and Dissertations

The purpose of this study was to investigate how adolescents in a small urban setting in the upper Midwest use alternative modes of written communication (i.e., text messaging, email, instant messaging, and social networking) for discourse purposes in order to identify ways in which these modes could be utilized by non speaking individuals. This study explored the factors related to choice of mode, including identity of the communication partner, number of communication partners, purpose of the communication, and personal preferences of the individual.

This qualitative study followed grounded theory methodology and used interviews as the primary means of data collection. …

Sex And Political Legitimacy: An Examination Of Byzantine Empresses (399 -1056 C.E.), Cheridan E. Austin

Honors College Theses

An examination of the three ways Empresses were able to assert political power in the Byzantine Empire from 399 - 1056

Infections And Inequalities: A Cultural Perspective On Theeconomic, Political, And Social Influences That Govern Medical Care In Haiti, Herby Jeanty

Honors College Theses

Medicine is a pivotal aspect in life for any individual. It provides a guideline for an individual well-being, along with nurturing and caring responsibilities he or she has towards another. However, statistics have shown that millions of men, women, and children around the world lack access to basic medical necessities. This paper will focus on diseases, such as tuberculosis that can be easily contained and cured often run rampant amongst families and neighbors living in the slums in Haiti. It will also explain the economic discrepancies, political imbalance, and social issues that govern health care in developing countries such as …

Learned Communication Within A Patient Centered Medical School Curriculum, Susan J. Splichal

Theses and Dissertations

No abstract provided.

Transformative Pedagogy In Conversation: The Role Of Instructor Interventions In Peer Feedback For Speech Outline Development, Michael J. Stasio

Communication & Leadership Dissertations and Theses

This study investigated the efficacy of written and oral peer feedback used as an instructional strategy to support student learning in speech outline development. One hundred seven students from a California Community College participated in a mixed-methods evaluative quasi-experimental study conducted in a two-fold process with both quantitative and qualitative measures. Existing literature on peer-centered learning has various gaps. These include four areas of uncertainty: implementation interventions, trust, self-perceived benefits, and contexts. Overall perceived learning benefits and four specific instructor intervention variables were measured in this study: (1) creation of a safe space for collaboration, (2) student training on the …

Assessing The Demand For Simplified Stormwater Modeling Tools Within The Design Profession To Facilitate The Adoption Of Sustainable Stormwater Practices, Austin Malone Moore

Theses and Dissertations

Stormwater runoff is a major environmental concern, particularly in urban environments. Trends in managing stormwater have evolved (and continue to evolve) from a quantity only approach into a sustainable approach, which integrates quantity, quality, the environment, and aesthetics. Best management practices (BMPs) and Low Impact Development (LID) are two well-documented techniques capable of managing to sustainable standards. There are a number of stormwater models available to design professionals today. However, there are few which integrate site-scale BMP/LID analysis in a simplified fashion. The purpose of this study is to determine if there is a demand in the design profession for …

Effects Of Dietary Fat On Resistant Starch Fermentation In Mice, Diana Badkoobeh

Honors Capstones

No abstract provided.