Digital Commons Network^™

Cell Culture Method And Apparatus For Mechanically Stimulating Cells, Seth W. Donahue

Michigan Tech Patents

A cell culture assembly and a method for culturing cells that provide mechanical stimulation to cells. The cell culture assembly can include a flow chamber positioned in a fluid path and a support comprising cells positioned within the flow chamber to expose the cells to the fluid path. The cell culture assembly can further include a means for producing a steady flow of fluid in the fluid path, and a means for producing an oscillatory flow of fluid in the fluid path simultaneously with producing the steady flow of fluid in the fluid path to mechanically stimulate the cells. The …

Distribution Of A Novel Binding Site For Angiotensins Ii And Iii In Mouse Tissues, Felicia M. Rabey, Vardan T. Karamyan, Robert C. Speth

HPD Articles

A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding site occurs in other mouse tissues, 8 tissues were assayed for expression of this binding site by radioligand binding assay and compared with the expression of this binding site in the forebrain. Particulate fractions of homogenates of testis, epididymis, seminal vesicles, heart, spleen, pancreas, lung, skeletal muscle, and forebrain …

New Undergraduate Course Explores Intersection Of Math And Biology, Staff

Browse All News

No abstract provided.

High-Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis And Method Of Generating The Same, Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai

Pharmaceutical Sciences Faculty Patents

A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, …

Daily Eastern News: June 08, 2010, Eastern Illinois University

June

No abstract provided.

Development Of Novel Image Analysis Methods For The Morphological Quantification Of Filamentous Fungi, David Barry

Doctoral

Mycelial morphology is a critically important process property in fermentations of lamentous micro-organisms, as particular phenotypes are associated with maximum productivity. The design of systems capable of rapidly and accurately characterising morphology within a given process represents a signi cant challenge to biotechnologists, as the complex phenotypes that are manifested are often not easily quanti ed. A system has been developed for high-resolution characterisation of lamentous fungal growth, using membrane immobilization and fully-automatic imageprocessing software. The system has been used to quantify the early-stage hyphal di erentiation of Aspergillus oryzae on solid substrates, by measuring spore projected area and circularity, …

Independent - Jun. 8, 2010, Victoria A Tullock

The Independent (1988-present)

No abstract provided.

Graduate Programming For Working Professionals: What Makes A Difference?, Christine G. Springer

Public Policy and Leadership Faculty Presentations

Education: A Graduate Degree Designed for New and Working Professionals

Mission: To provide a well-rounded graduate learning experience to current and future crisis and emergency management leaders for effectively addressing natural, intentional and technical disasters

On The Use Of Cartographic Projections In Visualizing Phylogenetic Treespace, Mark J. Clement, Quinn O. Snell, Kenneth Sundberg

Faculty Publications

Phylogenetic analysis is becoming an increasingly important tool for biological research. Applications include epidemiological studies, drug development, and evolutionary analysis. Phylogenetic search is a known NP-Hard problem. The size of the data sets which can be analyzed is limited by the exponential growth in the number of trees that must be considered as the problem size increases. A better understanding of the problem space could lead to better methods, which in turn could lead to the feasible analysis of more data sets. We present a definition of phylogenetic tree space and a visualization of this space that shows significant exploitable …

Council On Academic Affairs Minutes, Jun 17, 2010, Eastern Kentucky University

Council on Academic Affairs

No abstract provided.

Ocean Currents Help Explain Population Genetic Structure, Crow White, Kimberly A. Selkoe, James Watson, David A. Siegel, Danielle C. Zacherl, Robert J. Toonen

Biological Sciences

No abstract provided.

New Hampshire Water Resources Research Center: Program Evaluation Report Fiscal Years 2003 - 2007, William H. Mcdowell

NH Water Resources Research Center Scholarship

No abstract provided.

Interview With Dr. Roger Ray: Rollins Alumnus And Professor Of Psychology, Roger Ray, Wenxian Zhang

Oral Histories

Roger Dean Ray came to Rollins College in 1969 to teach psychology. Over his tenure, he has authored numerous publications and engaged in many experimental projects. Eventually, Ray became the head of the Department of Psychology and the founding director of the Quantitative Learning and Teaching Center at Rollins.

Born in New Castle, Indiana, in 1941, Ray earned his B.A. in mathematics and psychology from Rollins College. He then studied at the University of Wisconsin for two years before completing his Ph.D. in general experimental psychology at the University of Tennessee in 1969.

While at Rollins College, Ray engaged in …

Ada News - 06/07/2010, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Tobacco Mosaic Virus Based Thin Film Sensor For Detection Of Volatile Organic Compounds, Michael A. Bruckman, Jie Liu, Goutam Koley, Yu Li, Brian C. Benicewicz, Zhongwei Niu, Qian Wang

Faculty Publications

A thin film sensor for the detection of volatile organic compounds (VOC) was fabricated by deposition of oligo-aniline grafted tobacco mosaic virus (TMV) onto a glass substrate. The oligo-aniline motifs were conjugated onto the TMV surface by a traditional diazonium coupling reaction to tyrosine residues followed by Cu(I) catalyzed alkyne-azide cycloaddition (CuAAC) reaction. The modified TMV was easily fabricated into a thin film by directly drop coating onto a glass substrate. Upon integration of the glass substrate into a prototypical device, the virus-based thin film exhibited good sensitivity and selectivity toward ethanol and methanol vapour.

June 7, 2010, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

Transcriptomic Responses In Mouse Brain Exposed To Chronic Excess Of The Neurotransmitter Glutamate, Xinkun Wang, Xiaodong Bao, Ranu Pal, Abdulbaki Agbas, Elias K. Michaelis

Faculty and Staff Publications

Background: Increases during aging in extracellular levels of glutamate (Glu), the major excitatory neurotransmitter in the brain, may be linked to chronic neurodegenerative diseases. Little is known about the molecular responses of neurons to chronic, moderate increases in Glu levels. Genome-wide gene expression in brain hippocampus was examined in a unique transgenic (Tg) mouse model that exhibits moderate Glu hyperactivity throughout the lifespan, the neuronal Glutamate dehydrogenase (Glud1) mouse, and littermate 9 month-old wild type mice.

Results: Integrated bioinformatic analyses on transcriptomic data were used to identify bio-functions, pathways and gene networks underlying neuronal responses to increased Glu synaptic release. …

Investigating Executive Functions In Men Seeking Help For Hypersexual Behavior Using Neuropsychological Testing, Rory C. Reid

Theses and Dissertations

Patients seeking help for hypersexual behavior often exhibit features of impulsivity, cognitive rigidity, poor judgment, deficits in emotion regulation, and excessive preoccupation with sex. Some of these characteristics are also common among patients presenting with neurological pathology associated with executive dysfunction. These observations led to the current investigation in which differences across scores on objective neuropsychological tests of executive functioning were explored in a group of hypersexual male patients (n = 30) compared with a non-hypersexual community sample (n = 30) of men. Using multivariate statistics, differences between the groups were examined yielding significant differences on measures of hypersexuality. However, …

Celogentin C And Thioviridamide: Synthetic And Structural Studies, Dmitry Nikolayevich Litvinov

Theses and Dissertations

Project I Celogentin C, isolated by Kobayashi from the seeds of Celosia argentea in 2001 exhibits strong inhibitory behavior towards polymerization of tubulin – globular protein, which plays crucial role during the cell division cycle. We have attempted synthesis of the left-hand ring of celogentin C via the intramolecular alkylation and the Knoevenagel condensation strategies. Utilizing synthetic methodologies in the field of nitroacetyl chemistry, developed by Kornblum and Rajappa we have successfully prepared the intermolecular Knoevenagel condensation product – the late-stage precursor to the left-hand ring of celogentin C. Synthesis of this key intermediate subsequently led to the preparation of …

God, Huntington's Disease And The Meaning Of Life, Kenneth P. Serbin

At Risk for Huntington's Disease

No abstract provided.

Medical School Watercooler Newsletter - June 6, 2010, Lindsey Lyle

Watercooler Newsletter

This is the June 6, 2010 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• USA Medical Students To Receive White Coats June 26
• Dr. Karen Fagan: Her Journey Through ELAM Program
• Webinar for New LexisNexis Interface
• "The Challenges of Parkinson's Disease"
• NIH Funds Consortium to Develop Lab Models for Mitochondrial Diseases

Advanced Biofuels: Commercializing The Efficient Syngas-To-Ethanol Platform, Wes Bolsen

CO2 Summit: Technology and Opportunity

No abstract provided.

Abstracts Of Oral Presentations

CO2 Summit: Technology and Opportunity

Abstracts of Oral Presentations and Keynote Lectures

How To Make Cement And Concretes With Lower Co2 Emissions, Ellis Gartner

CO2 Summit: Technology and Opportunity

No abstract provided.

Enhanced Photosynthetic Growth, Biodiesel And Electricity Production Using C. Vulgaris And P. Putida/S. Cerevisiae, Pranabendu Mitra

CO2 Summit: Technology and Opportunity

No abstract provided.

Deafdigest: Gold Edition, June 6, 2010, Barry Strassler

DeafDigest Gold 2010

No abstract provided.

After The License Approval: How Analytics Can Keep You In The Market, Robert Sitrin

Vaccine Technology III

Getting a BLA approved for a new vaccine can be a daunting task, but keeping the market supplied after licensure of a successful vaccine can be an even bigger challenge. Analytics can play an important role in keeping product supplied to the marketplace, especially during the critical launch phase The talk will describe the application of analytical comparability to GARDASIL®, Merck’s novel new vaccine to prevent cervical cancer, in order to bridge product produced by a launch facility to that produced by a scale-up facility without the need for a clinical trial. This effort assured a smooth transition of supply …

A Vaccine Prototype Using Baculovirus Expression System For The Control Of Avian Influenza Virus, Mauricio Realpe, R. Mora, L. Castellanos, F. Robles, C. González-Hernández

Vaccine Technology III

A clade 1 sequence of H5 haemaglutinin from an Asian Avian H5N1 isolate was used as a the template to chemically synthetize a codon optimized version for expression in insect cells. A single clone was chosen for expression optimization and changes were introduced in order to maximize the amount of protein to be produced and to resemble another sequence demonstrated to be present in an isolate causing disease in Humans.

Preliminary analysis at lab scale have shown promising yields of the haemaglutinin using an activity titration assay and an ELISA-based detection method. Optimization of the cell seeding, MOI, and time …

Rabies Virus Glycoprotein (Rvgp) Expression In Drosophila S2 Cells And By Semliki Forest Virus (Sfv). Synthesis And Protection Studies, Carlos Pereira

Vaccine Technology III

Here we report the utilization of Drosophila melanogaster Schneider 2 (S2) cells and the Semliki Forest Virus (SFV) expression system to produce the rabies virus glycoprotein (RVGP). Although they represent different expression systems, they offer similar advantages in generating high-level expression of functional membrane proteins. They are both relatively easy and safe to handle and are scalable. RVGP synthesized by S2 cells and SFV carrying an mRNA coding for RVGP (SFV-RVGP) were assayed in mouse protection studies. We have constructed S2 gene vectors with the hygromycin selection gene (H) in which the RVGP gene is inserted under the control of …

Human Cells For Prostate Cancer Vaccine Therapy - The Impact Of Centrifugation Upon Key Product Quality Attributes, Michael Delahaye, Katherine Lawrence, Carol Chu Mike Hoare, Stephen Ward

Vaccine Technology III

Centrifugation is a unit operation used within a wide range of bioprocesses, including the production of a whole cell cancer vaccine to treat Hormone Refractory Prostate Cancer (HRPC) which has been tested in Phase II clinical trials. To quantify the effect of centrifugation-related stresses upon a whole cell vaccine population, a design of experiments (DOE) based investigation at micro-scale was implemented. Qualification of the cells may be by membrane integrity and by surface marker density as well as cytokines released and retained during bioprocessing. These and possibly other factors combine to affect the biopotency of the cells and their clinical …