Digital Commons Network^™

Prediction And Testing Of Biological Networks Underlying Intestinal Cancer, Vishal N. Patel, Gurkan Bebek, Mark R. Chance

Faculty Scholarship

Colorectal cancer progresses through an accumulation of somatic mutations, some of which reside in so-called "driver" genes that provide a growth advantage to the tumor. To identify points of intersection between driver gene pathways, we implemented a network analysis framework using protein interactions to predict likely connections - both precedented and novel - between key driver genes in cancer. We applied the framework to find significant connections between two genes, Apc and Cdkn1a (p21), known to be synergistic in tumorigenesis in mouse models. We then assessed the functional coherence of the resulting Apc-Cdkn1a network by engineering in vivo single node …

Mip/Mtmr14 And Muscle Aging, Scott K. Powers, Michael B. Reid

Physiology Faculty Publications

No abstract.

The Bg News September 1, 2010, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. September 1, 2010. Volume 101-Issue 9.

Nona-Arginine Facilitates Delivery Of Quantum Dots Into Cells Via Multiple Pathways, Yi Xu, Betty Revon Liu, Han Jung Lee, Katie Shannon, Jeffrey G. Winiarz, Tien-Chun Wang, Huey-Jenn Chiang, Yue-Wern Huang

Biological Sciences Faculty Research & Creative Works

Semiconductor quantum dots (QDs) have recently been used to deliver and monitor biomolecules, such as drugs and proteins. However, QDs alone have a low efficiency of transport across the plasma membrane. In order to increase the efficiency, we used synthetic nona-arginine (SR9), a cell-penetrating peptide, to facilitate uptake. We found that SR9 increased the cellular uptake of QDs in a noncovalent binding manner between QDs and SR9. Further, we investigated mechanisms of QD/SR9 cellular internalization. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/SR9, indicating that internalization is an energy-dependent process. Results from both the pathway inhibitors and …

Exploratory Analysis Of Pathogen Resistance Responsible Genetic Elements In Two Eudicots From Whole Genome Sequence, Aubrey Bailey

Theses

Agricultural products are becoming more homogenous in order to conform to consumer expectations. In doing so, they have become a high-risk target for widespread crop failure due to pathogens or other agricultural maladies. Presented here are a collection of tools and a work-flow for the wholesale detection and characterization of disease resistance-associated proteins from whole-genome sequences. These techniques were also adapted for identifying potential miRNA regulatory sequences. Putative R-gene sequences identified from the Malus x Domestica 'Golden Delicious' genome were acquired for verification against wild apple species. Resistance gene analogs were PCR amplified from R-gene associated domains (TIR, NBS, and …

(Wp 2010-04) Mäki On Economics Imperialism, John B. Davis

Economics Working Papers

This paper reviews Uskali Mäki’s epistemic analysis of economics imperialism formulated in terms of the science unification ideal and the three constraints on imperialism he develops. It then examines the phenomenon of 'reverse imperialism' associated with the influence of other fields on economics especially since 1980, and advances a core-periphery model of the identity of economics as a field made up of a collection of different research programs. The discussion returns to Mäki’s constraints framework to re-evaluate the 'economics' imperialism of individual research programs, and evaluates what we learn from Mäki’s three constraints. The paper concludes with a brief comment …

Japanese Science Fiction And Conceptions Of The (Human) Subject, Maria Poulaki

CLCWeb: Comparative Literature and Culture

In her article "Japanese Science Fiction and Conceptions of the (Human) Subject" Maria Poulaki discusses the crisis that almost all essentialist categorizations have been facing in late modernity, in the context of which science fiction texts offer fertile ground to investigate the transitions brought about with the intensified invasion of the "human self" by its "nonhuman other." The analysis of a Japanese science fiction film draws a seemingly paradoxical connection between the Japanese version of modernity and self-identity with the relevant "Western" articulations found in the work of Bruno Latour and Alain Badiou. This connection points at a broader re-conceptualization …

The Cinema Of Control: On Diabetic Excess And Illness In Film, Kevin L. Ferguson

Publications and Research

While not rare, films that do represent diabetes must work around the disease’s banal invisibility,and images of diabetics in film are thus especially susceptible to metaphor and exaggeration.This essay is the first to outline a diabetic filmography, discussing medical and cinematic strategies for visualizing the disease as well as how the illness informs family plots and heroic characters in horror films. Doing so, it participates in a larger discussion of the manner in which film images of ill or disabled groups sustain notions of “normalcy” by both representing and denying otherness.

Full Issue: Vol. 64, Issue 3

Sci-Tech News

No abstract provided.

The Biopax Community Standard For Pathway Data Sharing, E. Demir, M. P. Cary, S. Paley, K. Fukuda, C. Lemer, I. Vastrik, G. Wu, P. D’Eustachio, C. Schaefer, J. Luciano, F. Schacherer, I. Martinez-Flores, Z. Hu, V. Jimenez-Jacinto, G. Joshi-Tope, K. Kandasamy, A. C. Lopez-Fuentes, H. Mi, E. Pichler, I. Rodchenkov, A. Splendiani, S. Tkachev, J. Zucker, G. Gopinath, H. Rajasimha, R. Ramakrishnan, I. Shah, M. Syed, N. Anwar, O. Babur, M. Blinov, E. Brauner, D. Corwin, S. Donaldson, F. Gibbons, R. Goldberg, R. Hornbeck, A. Luna, P. Murray-Rust, E. Neumann, O. Reubenacker, M. Samwald, M. Van Iersel, S. Wimalaratne, K. Allen, B. Braun, M. Whirl-Carrillo, K. H. Cheung, Kam D. Dahlquist, A. Finney, M. Gillespie, E. Glass, L. Gong, M. Honig, O. Hubaut, D. Kane, S. Krupa, M. Kutmon, J. Leonard, D. Marks, D. Merberg, V. Petri, A. Pico, D. Ravenscroft, L. Ren, N. Shah, M. Sunshine, R. Tang, R. Whaley, S. Letovksi, K. H. Buetow, A. Rzhetsky, V. Schachter, B. S. Sobral, U. Dogrusoz, S. Mcweeney, M. Aladjem, E. Birney, J. Collado-Vides, S. Goto, M. Hucka, N. Le Novère, N. Maltsev, A. Pandey, P. Thomas, E. Wingender, P. D. Karp, C. Sander, G. D. Bader

Biology Faculty Works

Biological Pathway Exchange (BioPAX) is a standard language to represent biological pathways at the molecular and cellular level and to facilitate the exchange of pathway data. The rapid growth of the volume of pathway data has spurred the development of databases and computational tools to aid interpretation; however, use of these data is hampered by the current fragmentation of pathway information across many databases with incompatible formats. BioPAX, which was created through a community process, solves this problem by making pathway data substantially easier to collect, index, interpret and share. BioPAX can represent metabolic and signaling pathways, molecular and genetic …

Freshwater Fishes Of South Carolina By Fred C. Rohde, Rudolf G. Arndt, Jeffrey W. Foltz, And Joseph M. Quattro With Photographs By Fred C. Rohde-Book Review, Martin O'Connell

Earth and Environmental Sciences Faculty Publications

No abstract provided.

Noninvasive Fluorescence Monitoring Of Protoporphyrin Ix Production And Clinical Outcomes In Actinic Keratoses Following Short-Contact Application Of 5-Aminolevulinate, Christine B. Warren, Sara Lohser, Lauren C. Wene, Brian W. Pogue, Philip Bailin, Edward Maytin

Dartmouth Scholarship

Topical 5-aminolevulinic acid (ALA) is widely used in photodynamic therapy (PDT) of actinic keratoses (AK), a type of premalignant skin lesion. However, the optimal time between ALA application and exposure to light has not been carefully investigated. Our objective is to study the kinetics of protoporphyrin IX (P_pIX) accumulation in AK after short contact ALA and relate this to erythemal responses. Using a noninvasive dosimeter, P_pIX fluorescence measurements (5 replicates) were taken at 20-min intervals for 2 h following ALA application, in 63 AK in 20 patients. Data were analyzed for maximal fluorescent signal obtained, kinetic …

Selective Targeting Of Neuroblastoma Tumour-Initiating Cells By Compounds Identified In Stem Cell-Based Small Molecule Screens, Kristen M. Smith, Alessandro Datti, Mayumi Fujitani, Natalie Grinshtein, Libo Zhang, Olena Morozova, Kim M. Blakely, Susan A. Rotenberg, Loen M. Hansford, Freda D. Miller, Herman Yeger, Meredith S. Irwin, Jason Moffat, Marco A. Marra, Sylvain Baruchel, Jeffrey L. Wrana, David R. Kaplan

Publications and Research

Neuroblastoma (NB) is the most deadly extra-cranial solid tumour in children necessitating an urgent need for effective and less toxic treatments. One reason for the lack of efficacious treatments may be the inability of existing drugs to target the tumour-initiating or cancer stem cell population responsible for sustaining tumour growth, metastases and relapse. Here, we describe a strategy to identify compounds that selectively target patient-derived cancer stem cell-like tumour-initiating cells (TICs) while sparing normal paediatric stem cells (skin-derived precursors, SKPs) and characterize two therapeutic candidates. DECA-14 and rapamycin were identified as NB TIC-selective agents. Both compounds induced TIC death at …

Snip Journal Impact Indicator Accounts For Differences In Citation Characteristics And Database Coverage Between Properly Defined Subject Fields, Henk F. Moed

Against the Grain

No abstract provided.

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Against the Grain

No abstract provided.

A Consilient Science And Humanities In Mcewan's Enduring Love, Curtis D. Carbonell

CLCWeb: Comparative Literature and Culture

In his article "A Consilient Science and the Humanities in McEwan's Enduring Love" Curtis D. Carbonell provides a reading of a Third Culture novel that foregrounds the relationship of the sciences and the humanities. In Ian McEwan's novel we see a perfect example of how literary thinkers are listening to the world of science and speaking to it in return. This article responds to Stephen Greenberg's ideas about how Neo-Darwinian themes in the novel point to social themes by arguing that what underlies both of these is a deeper structure: the tension between C.P. Snow's Two Cultures, which is only …

Issues In Vendor/Library Relations-You Are Invited, Bob Nardini

Against the Grain

No abstract provided.

The Metaphysics Of Electronic Being, Michał Ostrowicki [Aka Sidey Myoo]

CLCWeb: Comparative Literature and Culture

In his article "The Metaphysics of Electronic Being" Michał Ostrowicki discusses the electronic environment as a sphere of being. To this end, the notion of the "electronic sphere" is used as a subject of ontological analysis. Ostrowicki postulates that the problematics of the electronic sphere represents a part of ontology and designates it as "ontoelectronics." He makes a distinction made between an electronic image and an electronic being, thus indicating that they differ from each other in their existential status and thereby deny any metaphysical equivalence between the two. This distinction between an electronic image and an electronic being is …

Gender In Winterson's Sexing The Cherry, Paul Kintzele

CLCWeb: Comparative Literature and Culture

In his article "Gender in Winterson's Sexing the Cherry" Paul Kintzele examines the ways in which Jeanette Winterson's 1989 novel explores and critiques aspects of gender and sexuality. While acknowledging the importance of the performance theory of gender that derives from the work of Judith Butler, Kintzele contends that such an approach must be complemented with a psychoanalytic approach that insists on a particular distinction between sex and gender. Although some scholars map the sex/gender distinction onto the perennial nature/nurture binary and thus reduce sex to biology or anatomy, scholars of psychoanalysis such as Joan Copjec and Charles Shepherdson, read …

Artificial Bowel Sphincters For Severe Fecal Incontinence. Are They A Solution?, Ahmad M. Zubaidi

Saudi Medical Journal

Fecal incontinence is a debilitating and common problem with a profound effect on a patient9s well being medically, socially, and economically. Non-operative management of this condition includes dietary modification, antidiarrheal medications, and biofeedback. Patients with severe incontinence can be divided into 2 categories. The first group includes patients with an identifiable and isolated anatomic sphincter defect who can expect 80% short-term surgical success using overlapping sphincteroplasty. The second group is patients who will not benefit from sphincteroplasty; fortunately, they are not obligated to permanent stomas. Artificial bowel sphincter (ABS) implantation is a well-established surgical technique, offers a chance for continence, …

Dynamical (E, 2e) Studies Using Tetrahydrofuran As A Dna Analog, Christopher J. Colyer, Susan M. Bellm, B. Lohmann, G. Friedrich Hanne, Ola A. Al-Hagan, Don H. Madison, Chuangang Ning

Physics Faculty Research & Creative Works

Triple differential cross sections for the electron-impact ionization of the outer valence orbital of tetrahydrofuran have been measured using the (e, 2e) technique. The measurements have been performed with coplanar asymmetric kinematics, at an incident electron energy of 250 eV and at an ejected electron energy of 10 eV, over a range of momentum transfers. The experimental results are compared with theoretical calculations carried out using the molecular three-body distorted wave model. The results obtained are important for gaining an understanding of electron driven processes at a molecular level and for modeling energy deposition in living tissue.

Selection For Non-Amyloidogenic Mutants Of Islet Amyloid Polypeptide (Iapp) Identifies An Extended Region For Amyloidogenicity, Ayano Fox, Thibaut Snollaerts, Camille Errecart Casanova, Anastasia Calciano, Luiza A. Nogaj, David A. Moffet

Chemistry and Biochemistry Faculty Works

The aggregation of the 37-residue polypeptide IAPP, as either insoluble amyloid or as small oligomers, appears to play a direct role in the death of pancreatic β-islet cells in type II diabetes. While IAPP has been known to be the primary component of type II diabetes amyloid, the molecular interactions responsible for this aggregation have not been identified. To identify the aggregation-prone region(s), we constructed a library of randomly generated point mutants of IAPP. This mutant IAPP library was expressed in E. coli as genetic fusions to the reporter protein enhanced green fluorescent protein (EGFP). Because IAPP aggregates rapidly, both …

Using Insects To Promote Science Inquiry In Elementary Classrooms, Douglas A. Golick, Tiffany M. Heng-Moss, Marion D. Ellis

Department of Entomology: Faculty Publications

The University of Nebraska-Lincoln and Nebraska public schools created Bugs in the Classroom, a professional development initiative with the goal of empowering teachers to use insects in science inquiry instruction in elementary classrooms. The initiative included workshops for elementary educators on science inquiry and teaching with insects. This paper includes a description of the workshop as well as an evaluation of the impact of the workshop on participating teachers' knowledge of scientific inquiry, entomology knowledge, and inquiry practice. Also included are recommendations for similar professional development activities.

The Relation Between The Arterial Stress And Restenosis Rate After Coronary Stenting, Linxia Gu, Shijia Zhao, Aswini K. Muttyam, James M. Hammel

Department of Mechanical and Materials Engineering: Faculty Publications

Two commercially available stents (the Palmaz–Schatz (PS) and S670 stents) with reported high and low restenosis rates, respectively, have been investigated in this paper. Finite element models simulating the stent, plaque, and artery interactions in 3 mm stenosed right coronary arteries were developed. These models were used to determine the stress field in artery walls after stent implantation. The material properties of porcine arteries were measured and implemented in the numerical models. The stress concentration induced in the artery by the PS stent was found to be more than double that of the S670 stent. It demonstrated a good correlation …

Increased Plant Uptake Of Nitrogen From 15N-Depleted Fertilizer Using Plant Growth-Promoting Rhizobacteria, A. O. Adesemoye, H. A. Torbert, J. W. Kloepper

Department of Plant Pathology: Faculty Publications

Harmful environmental effects resulting from fertilizer use have spurred research into integrated nutrient management strategies which can include the use of specific microorganisms to enhance nutrient use efficiency by plants. Some strains of plant growth-promoting rhizobacteria (PGPR) have been reported to enhance nutrient uptake by plants, but no studies with PGPR have used ¹⁵N isotope techniques to prove that the increased N in plant tissues came from the N applied as fertilizer. The current study was conducted to demonstrate that a model PGPR system can enhance plant uptake of fertilizer N applied to the soil using different rates of …

My Life In Biology: Paul A. Johnsgard, Paul A. Johnsgard

Papers in Ornithology

I was born in 1931 in the very small town of Christine, North Dakota, on the Red River about 20 miles south of Fargo. My granddad owned a general store there, and my father worked in that store for as long as we lived in Christine, which was until 1939. These were the Depression years, and my major memories of that time are of hot dusty streets in the summer and bitterly cold winters, when I had to walk across town to school. ...

Waterfowl became increasingly important to me because of my mother's cousin "Bud" Morgan, who at that …

A Case Study In Unethical Transgressive Bioethics: "Letter Of Concern From Bioethicists" About The Prenatal Administration Of Dexamethasone., Laurence B Mccullough, Frank A Chervenak, Robert L Brent, Benjamin Hippen

Department of Pediatrics Faculty Papers

On February 3, 2010, a "Letter of Concern from Bioethicists," organized by fetaldex.org, was sent to report suspected violations of the ethics of human subjects research in the off-label use of dexamethasone during pregnancy by Dr. Maria New. Copies of this letter were submitted to the FDA Office of Pediatric Therapeutics, the Department of Health and Human Services (DHHS) Office for Human Research Protections, and three universities where Dr. New has held or holds appointments. We provide a critical appraisal of the Letter of Concern and show that it makes false claims, misrepresents scientific publications and websites, fails to meet …

B7-H3 And Cd133 Expression In Non-Small Cell Lung Cancer And Correlation With Clinicopathologic Factors And Prognosis, Yue-Hua Xu, Guang-Bo Zhang, Jia-Min Wang, Hua-Cheng Hu

Saudi Medical Journal

OBJECTIVE: To detect the expression of B7-H3 and CD133 in human non-small cell lung cancer (NSCLC) specimens and lung benign lesions, and to evaluate the correlation between the 2 biomarkers and clinicopathologic features. METHODS: This is a case-control study of 102 tissue specimens collected from NSCLC participants undergoing thoracic surgery in the Second Affiliated Hospital of Soochow University, Suzhou, China, between January 2006 and December 2008. From the 102 patients, 25 adjacent non-cancer samples were verified pathologically as normal tissue (positive group), and 24 benign inflammatory lesion tissues were used as control (negative group). Specimens from 126 participants were stained …

Clinical Characteristics Of 74 Pandemic H1n1 Influenza Patients From Turkey. Risk Factors For Fatality, Emin E. Tutuncu, Baris Ozturk, Yunus Gurbuz, Asli Haykir, Irfan Sencan, Ferit Kuscu, Gulay Dede, Aysegul U. Kilic, Gonul C. Senturk

Saudi Medical Journal

OBJECTIVE: To evaluate the clinical characteristics and certain risk factors that may be associated with fatal outcome in patients with H1N1 influenza. METHODS: This retrospective study was conducted between October and December 2009 in the Department of Infectious Diseases and Clinical Microbiology, SB Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey. Data regarding the epidemiological and clinical characteristics of 74 hospitalized cases of confirmed pandemic H1N1 influenza were reviewed. RESULTS: The median age was 49 (18-83) years, and 34 (46%) were males. The most common symptom and signs on admission were cough (91.9%) and fever more than 38 degrees …

Effects Of Prostate Manipulation On Serum Total And Free Prostate Specific Antigen, And Free-To-Total Prostate Specific Antigen Ratio, Yun-Hua Lin, Yong-Guang Jiang, Ming-Chuan Li, Yong Luo, Jun-Sheng Wang

Saudi Medical Journal

OBJECTIVE: To evaluate the effects of the different types of manipulation on prostate total specific antigen (tPSA), free prostate specific antigen (fPSA), and free-to-total prostate specific antigen (f/tPSA). METHODS: A total of 160 males were enrolled from January 2006 to December 2009 in the Urology Department, Beijing Anzhen Hospital affiliated to the Capital Medical University, Beijing, China. Of these patients, 23 had digital rectal examination (DRE), 21 had urethral catheterization, 28 had rigid cystoscopy, 35 had prostate biopsy, 35 underwent transurethral resection of the prostate (TURP), and 18 underwent suprapubic prostatectomy. Blood samples were taken before, at 24 hours, and …