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Optimization And Toxocologic Effects Of Cancer Immuno-Electrogene Therapy Using A Tumor-Targeted Interleukin-12 Gene Construct, Scott Douglas Reed

LSU Doctoral Dissertations

This dissertation includes a comprehensive current review of reversible electroporation (EP) and other related physical gene transfection techniques; an overview of results of electrochemogene therapy (ECGT) used to treat naturally occurring spontaneous neoplasms in dogs; and the results of comprehensive, pre-clinical toxicology testing of electrogene therapy (EGT) of a tumor-targeted version of interleukin-12 (IL-12) in mice. Intralesional bleomycin (BLM) and feline interleukin-12 (fIL-12) DNA injection combined with trans-lesional EP resulted in complete cure of two recurrent oral squamous cell carcinomas and an acanthomatous ameloblastoma in a series of six cases of spontaneous neoplasia in pet dogs. The three remaining dogs, …

Lipid Bilayer-Nanoparticle Interactions In Nanotoxicology And Nanomedicine, Yanjing Chen

Open Access Dissertations

Nanoparticle (NP) interactions that may destabilize cell membranes and cause toxicity can also be used to design and control therapeutic assemblies. Fundamentally examining interactions between NPs and cell membranes is crucial to understanding NP toxicity mechanisms and the development of safe and non-toxic NP-based commercial and therapeutic applications. In this dissertation it is demonstrated that self-assembled lipid bilayers can be employed as model membranes to determine NP interact ions. First, protocols were developed for examining hydrophobic and hydrophilic NP-bilayer interactions using lipid bilayer vesicles or liposomes. Second, interactions between liposomes and NPs were characterized by examining changes in liposome structure, …

The Effect Of Perinatal Morbidity, Child Health And Maternal Influences On Social Competence At Age 12 Years In Formerly Preterm Infants, Nicole C. Smith

Open Access Dissertations

Prematurity is a national problem with rates climbing to the highest levels in four decades. Social competence is a critically important skill for children to master in order to become socially competent adults. Social competence is not fully studied in children born prematurely. Yet they are at risk for poorer social competence due to their physical, cognitive, motor and developmental difficulties. The purpose of this study was to examine the effect of perinatal morbidity, child health, and maternal influences on social competence at age 12 in a sample of formerly preterm infants and a full term comparison group. Two hypotheses …

Physical And Chemical Interactions Between Marine Phytoplankton And Particle-Colonizing Bacteria, Jason Robert Graff

Open Access Dissertations

It is inherently difficult to study the direct interactions between marine microbes due to the size of the organisms and microscale environments in which they exist. Recent evidence suggests that the direct associations between marine phytoplankton and particle-colonizing bacteria can influence marine microbial community succession, microalgal primary production, toxin production by marine phytoplankton, and have potential implications for human health. The goal of this dissertation was to elucidate some of the physical and chemical mechanisms that regulate microbial interactions, particularly the colonization of phytoplankton and other marine particles by bacteria. To accomplish these goals, I used a creative and multi-disciplinary …

Critical Moments In Classical Literature [Review], Lawrence Kim

Classical Studies Faculty Research

Critical Moments in Classical Literature is a curious book; deeply learned, elegantly written, and filled with subtle observations on a vast array of texts, but also somewhat diffuse, elusive, and in the end frustrating. On the face of it, the subtitle, Studies in the Ancient View of Literature and its Uses, is a good description of the book’s six chapters, each focused on a text constituting a ‘critical moment’ in ancient literary criticism: (1) Aristophanes’ Frogs, (2) Euripides’ Cyclops, (4) Dionysius of Halicarnassus’ On Imitation, (5) Longinus’ On the Sublime, and (6) Plutarch’s How the …

Analytical Evaluation Of Dispersion Of Exhaust From Rooftop Stacks On Buildings, Ted Stathopoulos, Hajra Bodhisatta, Ali Bahloul

Rapports de recherche scientifique

Cliquez ici pour la version française de ce rapport

« Dispersion » permet de décrire la façon dont les effluents des cheminées ou d’autres émissions des bâtiments sont transportés et dilués par le vent soufflé à travers un regroupement de bâtiments ou ses environs immédiats. La pollution atmosphérique est extrême lorsque l’air présente un niveau de pollution excessif pendant plusieurs heures ou plusieurs jours, et il peut en résulter un grand inconfort, des maladies et même des décès chez les personnes les plus vulnérables. Il y a forte probabilité de pollution extrême en présence d’inversions thermiques persistantes et de vents …

Practical Use Of Srtm Digital Elevation Dataset In The Urban-Watershed Modeling

Journal of Spatial Hydrology

The advent of satellite-based elevation dataset acquired by Shuttle Radar Topography Mission (SRTM) made new and novel techniques possible to model hydrological process in midsize to large scale watersheds. This application is important in regions with poor photogrammetric coverage and land use in appropriate scale. Watersheds are natural integrators of hydrological processes and as such require an integrated approach in data analysis and modeling. The first task is to delineate watershed boundaries accurately using terrain dataset. This research assessed the effectiveness of satellite base Digital Elevation Model (DEM) with photogrammetric base DEM in the Upper Klang watershed which is a …

On Corrupting The Youth, John Gee

Review of Books on the Book of Mormon 1989–2011

Review of Chrsitian Smith. Souls in Transition: The Religious and Spiritual Lives of Emerging Adults. and Review of Mark D. Regenerus. Forbidden Fruit: Sex and Religion in the Lives of American Teenagers.

Revealing The Role Of Receptor Wsx1: A Double-Edged Sword In Tumor Progession, Denada Dibra

LSU Doctoral Dissertations

Tumor initiation and progression are dependent on both aberrant gene expression in tumor cells and the communication between tumor cells and its micro- and systemic microenvironment. Many tumor suppressor genes and oncogenes have been characterized to suppress or promote tumor growth, but fewer genes in tumors are well-characterized as interacting with immune cells in the host to promote or inhibit tumor growth. The interleukin (IL) 27 receptor WSX1 is expressed in immune cells and induces an IL27-dependent immune response. Opposing this conventional dogma, our initial results reveal a much higher level of WSX1 expression in multiple types of epithelial tumor …

Effects Of Delays To Response Blocking When Used As Treatment For Problem Behavior Maintained By Automatic Reinforcement, Megan Leigh Kliebert

LSU Master's Theses

Response blocking and response interruption are common interventions for problem behavior maintained by automatic reinforcement in the treatment literature, but these interventions may be extremely challenging for caregivers to implement with fidelity (i.e., immediately blocking each instance). We evaluated the effects of challenges to the procedural integrity of response blocking and interruption procedures upon the maintenance of treatment effects for problem behavior maintained by automatic reinforcement for two young men by measuring aberrant behavior under several conditions including a baseline condition, an immediate response blocking or interruption condition, and delayed response blocking or interruption conditions (e.g., 3-s, 15-s, and 30-s …

Cell Extract-Based Reprogramming Of Somatic Cells, Laura Whitney Coley

LSU Master's Theses

The differentiation potential of adult stem cells (ASC) has long been thought to be limited to cell lineages present in the organ from which they are derived; however, several studies have challenged this notion by demonstrating that some ASC exhibit a remarkably high degree of plasticity. Unlike terminally differentiated somatic cells, the less differentiated state of ASC can assume the functional phenotypes and expression profiles of cells unique to other tissues. The expansive repertoire of differentiation potential exhibited by ASC suggests these cells possess characteristics similar to pluripotent cells, including epigenomic regulatory pattern. Therefore, ASC may be better equipped for …

The Role Of Bone Marrow In Siv Pathogenesis Using The Rhesus Macaque Model, Amy F. Gill

LSU Doctoral Dissertations

CD4+ memory T cells are depleted in mucosal tissues post human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection without restoration to pre-infection levels during progressive course of disease. Bone marrow (BM) as a hematopoietic organ has been investigated for hematologic and morphologic changes during HIV infection. However, BM as a primary lymphoid tissue during HIV infection has been poorly characterized. We proposed BM was also a site of CD4+ T cell depletion driven by increased apoptosis during progressive HIV disease. We chose to investigate bone marrow changes using the premier non-human primate Rhesus macaque SIV model for the …

In Vitro And In Vivo Evaluation Of A Brucella Putative Hemagglutinin, Lauren E. Duhon

LSU Doctoral Dissertations

Brucellosis, a zoonotic disease caused by Brucella spp., presents both health and economic difficulties for livestock, wildlife, and humans. While brucellosis is nearly eradicated in the United States, the disease remains detrimental in many countries worldwide. Attempts to produce a safe and effective small ruminant vaccine have been met with limited success. The current vaccine for bovine brucellosis in the United States is B. abortus RB51. This strain transiently colonizes the host and induces a cell-mediated immune response. Levels of protection have not been demonstrated in goats and thus it is considered a relatively poor caprine vaccine that probably does …

Patterns And Mechanisms Of The Exploitation Of Mutualisms, Ellen Mcgrail Reid

LSU Master's Theses

Mutualisms are reciprocally exploitative interactions providing net benefits to both partners. These interactions can be exploited, in turn, by individuals that take advantage of benefits offered by one or both partners in a mutualism, while offering no benefits in return. For many mutualist-exploiter interactions the mechanisms allowing exploitation, and the maintenance of mutualisms in the face of exploitation, are still poorly understood. Here I describe manipulative field and laboratory experiments to investigate the mechanisms used by an exploiter to invade an ant-plant mutualism. I tested two non-mutually exclusive hypotheses for how a coreid (Mozena sp., Hemiptera: Coreidae) feeds on mymecophytic …

Dissolution Of Chitosan In Ionic Liquids For Acylation Chemistry And Formation Of Cellulose-Chitosan Blends, Cristina Stefanescu

LSU Doctoral Dissertations

Dissolution of chitosan in ionic liquids was accomplished. It has been shown that 1-butyl-3-methylimidazolium acetate (BMIMAc) is a better solvent than 1-butyl-3-methylimidazolium chloride (BMIMCl). Dissolution of chitosan in BMIMCl required a prior regeneration of chitosan from 1% acetic acid solution. In the case of BMIMAc, both dried chitosan and regenerated chitosan from acetic acid solution have been dissolved in a relatively short amount of time. While concentrations up to 2 wt% of chitosan in BMIMCl could be obtained, concentrations of 10 wt % of chitosan in BMIMAc were realized. Homogeneous phthalation and benzoylation of chitosan were achieved in these ionic …

Caged Morpholino Oligonucleotide For Control Of Gene Expression In Zebrafish, Chad Michael Jarreau

LSU Master's Theses

To control gene expression in vivo with spatial and temporal precision remains a significant hurdle in laboratory studies of development as well as clinical genetic therapies. Here we demonstrate such control over gene expression by use of photochemistry to reversibly inactivate the hybridization of a nucleic acid analog used for specific protein knockdown. A morpholino oligonucleotide, commonly used for knockdown of protein expression in developmental studies, was “caged” using carbodiimide conjugate chemistry which yielded photocleavable adducts that can be removed with light exposure. Photochemical inactivation approaches to produce caged molecules have been used to control the spatiotemporal activity of biomolecules …

Parameterization Of Microwave Assisted Oil Extraction And Its Transesterification To Biodiesel, Akanksha V. Kanitkar

LSU Master's Theses

Currently, a majority of the world’s energy needs are met through use of fossil fuels, petroleum, coal and natural gas. The depletion of petroleum reserves, rising and extremely volatile crude oil prices, and environmental concerns have led to search for renewable and environmentally friendly fuels. The ultimate goal of this research was to develop, test and optimize a batch microwave system using traditional and alternate non-food feedstocks. Microwave assisted extraction (MAE) of oil from conventional feedstocks (soybeans and rice bran) and an alternative feedstock (Chinese tallow tree (CTT) seeds) was carried out. The study concluded that MAE of oil is …

Quantification Of Energy Intake Using Food Image Analysis, Sertan Kaya

LSU Master's Theses

Obtaining real-time and accurate estimates of energy intake while people reside in their natural environment is technically and methodologically challenging. The goal of this project is to estimate energy intake accurately in real-time and free-living conditions. In this study, we propose a computer vision based system to estimate energy intake based on food pictures taken and emailed by subjects participating in the experiment. The system introduces a reference card inclusion procedure, which is used for geometric and photometric corrections. Image classification and segmentation methods are also incorporated into the system to have fully-automated decision making.

Cross-Race Advising Relationships: The Role Of Race In Advising Relationships Between Black Doctoral Student Protégés And Their White Faculty Advisors, Marco Javon Barker

LSU Doctoral Dissertations

The purpose of this phenomenological study was to examine the role of race in cross-race advising relationships between White faculty advisors and their Black doctoral student protégés. I examined the racial context of doctoral education experiences and relationships between doctoral students and faculty. Blacks with doctoral degrees comprise only 0.3% of those 18 years and older and only 3.5% of those with doctoral degrees (U.S. Census, 2000). Although there has been an increase in the number of Blacks enrolling in doctoral programs (Cook & Cordova, 2006), Nettles and Millett (2006) found that Blacks and Latin Americans have higher attrition rates …

Chemistry Of Rhenium(I) Tricarbonyl Complexes Of Biomedical Relevance, Theshini Perera

LSU Doctoral Dissertations

fac-[Re^I(CO)₃L]ⁿ complexes serve as models for short-lived fac-[^99mTc^I(CO)₃L] imaging tracers. Dangling groups on L, needed to achieve desirable biodistribution, complicate the NMR spectra, which are not readily understood. In fac-[Re^I(CO)₃L]⁺ with less complicated L, NH groups (exo-NH) projecting toward the L face sometimes showed an upfield shift attributable to steric shielding of the exo-NH group from the solvent by the chelate rings. To investigate whether exo-NH groups in six-membered rings exhibit the same effect and whether the …

Modeling The Impacts Of Pulsed Riverine Inflows On Hydrodynamics And Water Quality In The Barataria Bay Estuary, Anindita Das

LSU Doctoral Dissertations

Eutrophication and coastal wetland loss are the major environmental problems affecting estuaries around the world. In Louisiana, controlled diversions of the Mississippi River water back into coastal wetlands are thought to be an important engineering solution that could reverse coastal land loss. There are concerns, however, that freshwater diversions may increase nutrient inputs and create severe eutrophication problems in estuaries and wetlands adjacent to the diversion sites. My dissertation research concerns modeling the effects of the observed and hypothetical freshwater diversion discharges on the hydrodynamics, salinity and water quality in the Barataria estuary, a deltaic estuary in south Louisiana. This …

Understanding The Use Of Barrier Islands As Nesting Habitat For Louisiana Birds Of Concern, Cecilia Marie Leumas

LSU Master's Theses

Colonial nesting seabirds are threatened by habitat loss and degradation, human disturbance, predation, and climate change. Several species of conservation concern concentrate high percentages of their total U.S. populations in Louisiana breeding colonies. We studied seabirds, including Royal Terns, Sandwich Terns, and Black Skimmers, nesting on Isles Dernieres barrier islands along the Gulf coast of Louisiana. Two of the four islands in this chain host extensive seabird colonies and two do not. We used an experimental approach to test the hypothesis that large terns and skimmers are prevented from nesting on Trinity Island, the largest of the Isles Dernieres, by …

Evolutionary Dynamics Of Bird Populations In Southeast Asia, Haw Chuan Lim

LSU Doctoral Dissertations

A confluence of factors determines the biological diversity we observe today. Here, I conducted three investigations of the historic, geographic and ecological factors that shaped the morphological and genetic diversity of rainforest birds in Southeast Asia. (1) In the Oriental Dwarf Kingfisher (Ceyx erithaca) complex, the birds are highly polymorphic in plumage. Quantitative analysis of this variation indicated that a large proportion of birds in Sumatra, the Malay Peninsula and Borneo have plumages intermediate between the northerly black form from mainland Asia and the southerly rufous form from Java. Phylogenetic analyses indicated that birds from continental Southeast Asia (north of …

Resistant Starch And Sodium Butyrate Reduce Body Fat In Rodents, Kirk Adam Vidrine

LSU Master's Theses

Introduction: Obesity levels in the United States have significantly increased in the last forty years. Lifestyle and pharmacological treatments have been largely ineffective in treating obesity for most people. Both Resistant Starch (RS) and Dietary Sodium Butyrate (SB) are bioactivties which have shown the ability to decrease body fat levels of rodents without increasing physical activity or decreasing energy intake. Glucagon-like peptide 1 (GLP-1) and peptide YY (PYY) are gut hormones that may be involved in increased energy expenditure at a cellular level with dietary RS and SB. Objective: To discern if SB and RS both work through the increase …

Analysis Of Media Agenda-Setting Effect On Consumer Confidence In The Safety Of The U.S. Food System Across Consumer Segments, Abhishek Bhagwat Bharad

LSU Master's Theses

Recent food recalls and food scares in the United States have increased consumers’ risk perceptions about food borne illness and decreased their confidence in the safety of the U.S. food supply. Results from a continuous tracking of consumer confidence and media coverage of food safety events over a 67 week period between May 2008 and August 2009 are reported in the study. Factor analysis is performed on consumer characteristic statements to identify seven factors. Factor scores for these seven factors are used as inputs in a consumer segmentation procedure. A two step segmentation approach, hierarchical cluster analysis followed by partition …

Value-Added Processing Of Rice And Rice By-Products, Rebecca C. Schramm

LSU Doctoral Dissertations

World competition has encouraged United States rice farmers and rice mills to be efficient in farming and production practices. Efforts to augment economic competitiveness include development of new varieties, improvements in milling practices, and identification of uses for rice products and by-products. The research detailed in this dissertation adds to the body of knowledge in milling practices and identification of uses for rice bran. To improve the prediction of milled rice quality at industrial scale, correlations for milling quality among laboratory, pilot, and industrial scale mills were identified for Clearfield 161. Final industrial product whiteness was ten points higher than …

Data Transfer Scheduling With Advance Reservation And Provisioning, Mehmet Balman

LSU Doctoral Dissertations

Over the years, scientific applications have become more complex and more data intensive. Although through the use of distributed resources the institutions and organizations gain access to the resources needed for their large-scale applications, complex middleware is required to orchestrate the use of these storage and network resources between collaborating parties, and to manage the end-to-end processing of data. We present a new data scheduling paradigm with advance reservation and provisioning. Our methodology provides a basis for provisioning end-to-end high performance data transfers which require integration between system, storage and network resources, and coordination between reservation managers and data transfer …

Fabrication, Characterization, Modeling And Testing Of A Nanostructured Bulk Thermoelectric Cooler, Dinesh Pinisetty

LSU Doctoral Dissertations

New generation micro/nano devices are emerging to monitor, control and act on living systems. Particularly, in the field of cryobiology, there is a need to monitor and control temperature at the cellular level. An important step towards achieving this aim is to fabricate a novel bulk nanostructured thermoelectric cooler (TEC). As a first step towards achieving efficient localized control of temperature in biological systems, Bismuth-telluride (Bi2Te3) and Antimony-Telluride (Sb2Te3) arrays of nanowires and nanotubes were fabricated, characterized and modeled. A thermal conductivity model originally developed by Dames and Chen for superlattice nanowires was extended to nanotubes. Based on this model …

The Returns And Volatility Of Agribusiness Stocks: How Do They Measure Up To Non-Agribusiness Stocks?, Benjamin Michael Clark

LSU Master's Theses

Little literature exists that analyzes the historical risk and return associated with investing in the US agricultural sector. However, several observable trends have recently made investing in the US agricultural sector an area of interest among investors. Stock price indexes are a commonly used tool for summarizing the historical performance of a specific sector. A market-capitalization weighted stock price index is created to represent the largest US agribusiness firms since 1970, using the US Census Standard Industrial Classification system and the Economic Research Service’s definition of agribusiness. Geometric returns, standard deviation, Sharpe Ratio, and beta values are calculated for one, …

Effects Of Meal Timing On Anabolic Hormone Status And Energy Metabolism In Neonatal Dairy Calves, Katherine Claire Simon

LSU Master's Theses

Twelve neonatal Holstein bull calves (38.52 ± 5.87 kg) were fed milk replacer at a fixed or varied meal time to determine the effects on metabolic hormone secretion, average daily intake, growth, and energy metabolism. Body weights were measured every two weeks from birth to 8 weeks. Rumen fluid was collected every two weeks from week 2 through 8. Serial blood collections were conducted every two weeks from week 2 through 8. Blood was collected, beginning at 0530, at 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, and 150 minutes. Plasma was analyzed for ghrelin, leptin, growth hormone …