Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

2010

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 16411 - 16440 of 18896

Full-Text Articles in Entire DC Network

2010-2011 Undergraduate Catalog, Armstrong Atlantic State University Jan 2010

2010-2011 Undergraduate Catalog, Armstrong Atlantic State University

Armstrong Office of the Registrar: Undergraduate Catalogs (Archived)

Academic catalog for Armstrong Atlantic State University.

Go to article

A Probabilistic Cell Model In Background Corrected Image Sequences For Single Cell Analysis, Nezamoddin N. Kachouie, Paul Fieguth, Eric Jervis Jan 2010

A Probabilistic Cell Model In Background Corrected Image Sequences For Single Cell Analysis, Nezamoddin N. Kachouie, Paul Fieguth, Eric Jervis

Mathematics and System Engineering Faculty Publications

Background: Methods of manual cell localization and outlining are so onerous that automated tracking methods would seem mandatory for handling huge image sequences, nevertheless manual tracking is, astonishingly, still widely practiced in areas such as cell biology which are outside the influence of most image processing research. The goal of our research is to address this gap by developing automated methods of cell tracking, localization, and segmentation. Since even an optimal frame-to-frame association method cannot compensate and recover from poor detection, it is clear that the quality of cell tracking depends on the quality of cell detection within each frame.Methods: …

Go to article

Why Beauty Still Cannot Be Measured, Ossi Naukkarinen Jan 2010

Why Beauty Still Cannot Be Measured, Ossi Naukkarinen

Contemporary Aesthetics (Journal Archive)

This article focuses on the question of whether the latest results achieved in sciences such as evolution studies and brain research can help us understand the nature of aesthetic judgments. It suggests that such approaches may offer interesting insights for understanding many problems in aesthetics, but for clarifying aesthetic judgments one needs a philosophical point of view. Aesthetic judgments cannot be proven right or wrong by scientific methods, and beauty or other aesthetic qualities cannot be directly measured. The “method” of both making and analyzing aesthetic judgments is discussion, and the article clarifies why this is still the case, even …

Go to article

Terrorism's Proscription And Core Elements Of An Objective Definition, Jordan J. Paust Jan 2010

Terrorism's Proscription And Core Elements Of An Objective Definition, Jordan J. Paust

Santa Clara Journal of International Law

This essay identifies core elements of an objective definition of terrorism as well as various words and phrases that others have used that are not part of an objective definition. In particular, an objective definition of terrorism must involve an intent of the perpetrator to produce a terror outcome as well as the existence of a terror outcome. The international community has recognized that terrorism is an international crime at least since 1985, but the community has been unable to agree on a definition or various core elements of a definition. This essay provides a possible basis for agreement by …

Go to article

The Impact Of Teacher Assigned But Not Graded Compared To Teacher Assigned And Graded Chemistry Homework On The Formative And Summative Chemistry Assessment Scores Of 11th-Grade Students With Varying Chemistry Potential, Jennifer L. Wilson Jan 2010

The Impact Of Teacher Assigned But Not Graded Compared To Teacher Assigned And Graded Chemistry Homework On The Formative And Summative Chemistry Assessment Scores Of 11th-Grade Students With Varying Chemistry Potential, Jennifer L. Wilson

Student Work

The study analyzed 2005 posttest data compared to 2008 posttest data to determine student end of school year academic achievement outcomes across three academic levels (above average, average, and below average chemistry potential) and two teacher homework evaluation methods (assigned but not graded and assigned and graded) on teacher prepared 11th-grade assessments, district prepared 11th-grade assessment, and district graduation requirement physical science strand 11th-grade science Essential Learner Outcome assessment. Overall, results indicated that students with above average (n = 16), average, (n = 17) and below average (n = 14) chemistry potential whom were given teacher assigned and graded chemistry …

Go to article

Ecology Of Mayfly (Insecta : Ephemeroptera) Nymphs In Three Streams Of Ulu Gombak Forest Reserve, Peninsular Malaysia., Sree Gayathree Selvantharan Jan 2010

Ecology Of Mayfly (Insecta : Ephemeroptera) Nymphs In Three Streams Of Ulu Gombak Forest Reserve, Peninsular Malaysia., Sree Gayathree Selvantharan

Student Works (2010-2019)

Three sampling stations with various environmental quality gradients in Ulu Gombak Forest Reserve, Selangor, Peninsular Malaysia were selected in this study: Sungai Gombak, Anak Sungai Gombak and Sungai Batu 19. A total of six genera of ephemeropteran nymphs were found from the mentioned streams: Baetis sp. (Baetidae) (232), Thalerosphyrus sp. (Heptageniidae) (925), Campsoneuria sp. (Heptageniidae) (1432), Neurocaenis sp. (Tricorythidae) (471), Platybaetis sp. (Baetidae) (12) and Prosopistoma sp. (Prosopistomatidae) (1). The most dominant family of ephemeropteran nymphs is Heptageniidae while Neurocaenis sp. (Tricorythidae) were the only nymphs that showed significant difference in abundance between all three streams (p=0.05) using student t-test. …

Go to article

Response Surface Optimization Of Crystal Violet Decolorization By Pellets Of Pyncnoporus Sanguineus., Siti Suhana Sulaiman Jan 2010

Response Surface Optimization Of Crystal Violet Decolorization By Pellets Of Pyncnoporus Sanguineus., Siti Suhana Sulaiman

Student Works (2010-2019)

Ligninolytic enzymes of white-rot fungi have broad specificities and have been implicated in transformation and mineralization of organo-pollutant with chemical structure similar to lignin e.g. synthetic dyes. This study was carried out to optimize selected parameters for decolorization of Crystal violet by Pycnoporus sanguineus in shake flasks. The parameters studied were initial dye concentration (ppm), agitation speed (rpm) and process time. They were optimized using response surface methodology (RSM). Subsequent to the optimization, the decolorization process was scaled up in stirred tank reactor (STR). Effect of different geometry of impellers on the decolorization process was studied. Curved blade and angled …

Go to article

Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah Jan 2010

Isolation And Characterization Of Microsatellite Loci From Wild Striped Snakehead (Channa Striata)., Yasmeen Sameer Mahmoud Salah

Student Works (2010-2019)

Channa striata (better known as Haruan in Malaysia), is a freshwater species that is well known as a protein source and also for its pharmaceutical value among wounded patients. Unfortunately stocks have declined over the past few years, thus an in-depth study is needed to rule out genetic irregularities that might be causing the slow extinction of the species. This study was conducted for the purpose of studying the population diversity and genetic structure of Channa striata (striped snakehead) in seven different locations around the peninsular of Malaysia. Microsatellite markers were isolated and identified using Random Amplified Microsatellite (RAM) primers. …

Go to article

Municipal Solid Waste Management In Selected Malaysian Islands., Nagendran Periaiah Jan 2010

Municipal Solid Waste Management In Selected Malaysian Islands., Nagendran Periaiah

Student Works (2010-2019)

Rapid developments and tourism related activities in Islands have increased the per capita waste generation that led to increased waste disposal at landfill. To date, islands in Malaysia are generating approximately 400 metric tons of MSW. Approximately 60% of the waste are collected and disposed off in non-sanitary landfills. The remaining 35 % were illegally burned/ dumped and 5 % were dumped into ocean. 100% of landfills in Malaysian islands operate as mere open-dumps that lack proper lining system and without leachate treatment facilities. The vicinities bordering non-sanitary landfill sites became highly polluted due to certain harmful gases/ leachate generated …

Go to article

Synthesis, Characterization, Electrochemistry And Biological Properties Of Schiff Bases And Their Metal Complexes., Zuraini Kadir Jan 2010

Synthesis, Characterization, Electrochemistry And Biological Properties Of Schiff Bases And Their Metal Complexes., Zuraini Kadir

Student Works (2010-2019)

A series of new indole hydrazone Schiff bases and their metal complexes have been synthesized, characterized and evaluated for anti-ulcerogenic activity.The Schiff bases derived from indole-3-acetic acid hydrazide and 2-hydroxyacetophenone and their zinc(II), nickel (II) and copper complexes were synthesized by condensation in ethanol. The IR, 1H and 13C NMR, UV Visible data indicate that the hydrazone ligands are coordinated to zinc metal. Indole hydrazones coordinated to zinc(II), nickel(II) and copper(II) metals as dimer ligands. The IR spectrum of the free ligand indicates that in solid state the ligand remains in the keto form. Recrystallization of ligand and complex 5-methyl-2-hydroxyacetophenone …

Go to article

Water Quality Study Of Paya Indah Wetlands., Ridzuan Ahmad Jan 2010

Water Quality Study Of Paya Indah Wetlands., Ridzuan Ahmad

Student Works (2010-2019)

A study had been carried out on Paya Indah Wetlands’ water quality from December 2006 to February 2007. This was to determine the status of water quality. From the analysis on Water Quality Index (WQI) at 15 sampling stations, it was observed that WQI of Paya Indah at most stations were within polluted range. From the comparative study made on WQI at major lakes and canals between year 1996 and 2006/2007, it was observed that Canal C2 and Canal C4 show slight improvement (48.7 – 50.0 and 41.5 – 59.2 respectively). However, major lakes of Paya Indah shows decrease of …

Go to article

Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah Jan 2010

Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah

Student Works (2010-2019)

In vitro regeneration of Gerbera jamesonii Bolus ex. Hook f. was successfully carried out in this study. Petiole and leaf explants from 8-week-old aseptic seedlings were used as source of explants. Different response was observed when different explants were cultured on the culture media. MS (Murashige and Skoog, 1962) supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA was identified as the optimum medium for shoot regeneration, while petiole explant was identified as the most responsive explant in this study. Petiole explants regenerated shoots (94.3 } 2.5 %) and produced 9.3 } 0.6 shoots per explant. MS basal medium was …

Go to article

Studies Of Antioxidant And Angiotensin Converting Enzyme Inhibitory Activity Of Orthosiphon Stamineus (Misai Kucing)., Che Wan Imanina Che Wan Takwa Jan 2010

Studies Of Antioxidant And Angiotensin Converting Enzyme Inhibitory Activity Of Orthosiphon Stamineus (Misai Kucing)., Che Wan Imanina Che Wan Takwa

Student Works (2010-2019)

Abstract NA

Go to article

Isolation And Characterization Of Actinobacteria From Soil Samples Of Signy Island, Antarctica., Pan Shing Yi Jan 2010

Isolation And Characterization Of Actinobacteria From Soil Samples Of Signy Island, Antarctica., Pan Shing Yi

Student Works (2010-2019)

In this study, ninety five actinobacterial isolates were cultured from seven soils samples collected on Signy Island, maritime Antarctica. Nine different isolation media were used and the isolates plates were incubated at 15ºC for up to four months. SCN agar serves as the better media for the isolation of actinobacteria. Presence of actinobacteria in the soil samples was also detected using PCR with actinobacteria specific primers. The actinobacterial isolates were characterized based on morphology (Gram-stain, coverslip examination, colour grouping), analysis of DAP isomers, antibacterial activities and by molecular methods (ARDRA, analysis of 16S rRNA sequence and detection of NRPS gene). …

Go to article

Physiological And Biochemical Attributes Of Red-Fleshed Dragon Fruit, Hylocereus Polyrhizus., Punitha R. Vasanthan Jan 2010

Physiological And Biochemical Attributes Of Red-Fleshed Dragon Fruit, Hylocereus Polyrhizus., Punitha R. Vasanthan

Student Works (2010-2019)

An understanding of the postharvest parameters is essential for the successful market introduction of red-fleshed dragon fruit, Hylocereus polyrhizus, which has been recently developed as fruit crop. In this study, fruit at three different harvest maturities were evaluated in terms of physiological and biochemical aspects. Fruit which were harvested at complete red stage maintained their quality for up to one week. On the other hand, fruit harvested at early maturity attained the physical properties of fruit ripened on the plant (firmness reduced, peel colour changes, pH increased) but they contained noticeably lower concentrations of soluble sugars suggesting that fruit can …

Go to article

Marine Algae As A Source Of Photosensitisers For Photodynamic Therapy Of Cancer., Tang Yee Voon Jan 2010

Marine Algae As A Source Of Photosensitisers For Photodynamic Therapy Of Cancer., Tang Yee Voon

Student Works (2010-2019)

Photodynamic therapy (PDT) involves the intravenous administration of a photosensitiser that preferentially localises in cancerous tissues more than the surrounding normal tissues. Subsequent illumination of photosensitiser generates reactive oxygen species that cause irreversible photo-damage to the cancer cells. To date, most photosensitisers that have been approved for clinical use or are currently in clinical trials are cyclic tetrapyrroles which are derived or inspired by naturally occurring chlorophyll and its degradation products. Some non-tetrapyrrolic photosensitisers have also been found in nature suggesting that there are potential photosensitisers with interesting structures in nature. To this end, this thesis outlines the collection of …

Go to article

Ligand-Protein Interactions Of Inhibitors With Dengue Virus Type 2 Serine Protease : A Structural Study., Rozana Othman Jan 2010

Ligand-Protein Interactions Of Inhibitors With Dengue Virus Type 2 Serine Protease : A Structural Study., Rozana Othman

Student Works (2010-2019)

Dengue is a serious infectious disease that is endemic in over 100 countries. There has been an estimate of 50 million infection per year globally, with more than 2.5 billion people are at risk for epidemic transmission. Two principal illnesses associated with dengue are Dengue Fever (DF) and Dengue Haemorrhagic Fever (DHF). To date, there is no licensed vaccine or therapeutic drug available for these illnesses, although there have been reports of some vaccine candidates in clinical trials. Treatments have only been supportive thus far. This thesis describes part of our ongoing effort to search for a lead therapeutic agent …

Go to article

Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song Jan 2010

Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song

Student Works (2010-2019)

Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC2F7 lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 …

Go to article

Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin Jan 2010

Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin

Student Works (2010-2019)

This study was aimed to investigate the effects of physiological stresses such as pruning, shading, phloemic stress and plant hormones such as gibberellic acid (GA3) and naphthalene acetic acid (NAA) on the growth, development and flowering process of bougainvillea plants. The plants (Bougainvillea glabra and Bougainvillea spectabilis) were grown under prevailing conditions (temperature 21-32°C, maximum PAR 2100 μE m-2 s-1 and relative humidity of 60-90%) using completely randomized design. Field experiments as well as biochemical analysis were carried out at the Plant Physiology Garden and the Plant Physiology Laboratory, Institute of Biological Sciences, University of Malaya. Results on frequent pruning …

Go to article

The Effect Of Heating On The Antioxidants Content In Edible Oil., Premanand Krishnan Jan 2010

The Effect Of Heating On The Antioxidants Content In Edible Oil., Premanand Krishnan

Student Works (2010-2019)

There are many types edible oils with various brand found in the market. The types of oils involved in the analysis for the antioxidants content were palm oil, olive oil, canola oil, sunflower oil, soy bean oil, corn oil, gingerly oil, rice bran oil, mixed oil and ayurvedic oil. The method used to evaluate the amount antioxidants in this study was 2, 2-diphenyl-1-picrylhydrazyl (DPPH). In this test, the scavenging of the DPPH radicals was followed by monitoring the decrease in absorbance at 515 nm. Heating the oil samples to various temperatures shows negative effect on the antioxidants content. The antioxidants …

Go to article

The Thermal Characteristic Of Imipenem Encapsulated In Low And High Molecular Weight Of Polyethylene Glycol., Abdul Aziz Ismail Jan 2010

The Thermal Characteristic Of Imipenem Encapsulated In Low And High Molecular Weight Of Polyethylene Glycol., Abdul Aziz Ismail

Student Works (2010-2019)

Microencapsulation technique is widely used in pharmaceutical industries to control the release of a drug. Biodegradable polymer such as Polyethylene glycol (PEG) is frequently used as encapsulation material. This study has tried to produce microencapsulation product of Imipenem antibiotics in Polyethylene Glycol.Commercially available product called Tienam® was used as a major source of Imipenem. We used freeze-drying method to produce the microencapsulation products. The successful of producing of this product is confirmed by the yellowish appearance of the product whereby it shows the colour properties of Tienam® Thermal analysis using Differential Scanning Calorimeter (DSC) was used to characterize pure materials …

Go to article

Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan Jan 2010

Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan

Student Works (2010-2019)

Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, …

Go to article

Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong Jan 2010

Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong

Student Works (2010-2019)

Tectonic movement and climatic oscillations during the Cenozoic have had dramatic effect on the biota of the tropical rain forest. This study aims to reveal the phylogeography and evolutionary history of a Peninsular Malaysian endemic tropical timber, Neobalanocarpus heimii (Dipterocarpaceae), based on chloroplast DNA (cpDNA) variation. Fifteen haplotypes were identified from 10 intraspecific variable sites of five non-coding cpDNA regions: trnL intron, trnS-trnG spacer, trnG intron, trnK intron and psbK-trnS spacer. Two major genealogical cpDNA lineages of N. heimii were elucidated: a widespread southern and a northern region. The species is predicted to survive in multiple refugia during climatic oscillation: …

Go to article

Enterobacterial Repetitive Intergenic Consensus-Pcr (Eric-Pcr) Analysis Among Raw Vegetables Isolates Of Campylobacter Jejuni., Tuan Suraya Tuan Soh Jan 2010

Enterobacterial Repetitive Intergenic Consensus-Pcr (Eric-Pcr) Analysis Among Raw Vegetables Isolates Of Campylobacter Jejuni., Tuan Suraya Tuan Soh

Student Works (2010-2019)

A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for the presence of virulence genes and genetic diversity using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All C. jejuni isolates were obtained from the laboratory of Food Science and Biotechnology, University Putra Malaysia, Serdang, Selangor. When specific PCR amplification were determined, all raw vegetables isolates of Campylobacter jejuni contained cadF and ceuE genes. Whereas, 12 (12/20) isolates were positive cdtB and 6 (6/20) were positive cdtC. None of the C. jejuni isolates was cdtA positive. Using ERIC-PCR analysis, all 20 isolates of C. jejuni were …

Go to article

Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa., Hassan Mahmood Kzar Jindal Jan 2010

Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa., Hassan Mahmood Kzar Jindal

Student Works (2010-2019)

In recent times, researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help protect the human body against oxidative stress, and replace the synthetic additives that might be carcinogenic. In present study, the crude extracts of leaves and fruits from Ardisia crispa were investigated for their chemical constituents and antioxidant activity. Preliminary phytochemical screening of the crude extracts via TLC analysis revealed the presence of several secondary compounds mainly, terpenoids and phenolic compounds in addition to alkaloids and saponins. HPLC analysis showed the possibility of presence of gallic acid in the fruits …

Go to article

Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari Jan 2010

Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari

Student Works (2010-2019)

The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …

Go to article

Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah Jan 2010

Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah

Student Works (2010-2019)

The aim of this study was to isolate protein/peptide and secondary metabolites from plant and fermented sources. The extracts were used to test for antimicrobial and antioxidant properties. In the current study protein/peptide extracts of Allium sativum showed promising results with a 15mm inhibition zone against Escherichia coli, 28mm against Staphylococcus aureus, 16.3mm against Bacillus cereus and 9mm against Pseudomonas aeruginosa. The minimum bacteriocidal concentration (MBC) results showed that Allium sativum protein/peptide extract is only bacteriostatic and not bacteriocidal. Andrographis paniculata showed the most promising result for the ethanolic extracts with an inhibition zone of 14mm against B. cereus, 11.5mm …

Go to article

Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A Hussein Jan 2010

Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A Hussein

Student Works (2010-2019)

Conventional techniques for removing dissolved heavy metals are only practical and cost-effective when applied to high strength wastes with heavy metal ion concentrations greater than 100 ppm. The possibility of using a non-living algal biomass to solve this problem was studied. Lead(II) was used in this study because it has been reported to cause several disorders in human. The non-living algal biomass was obtained from the filamentous green alga Spirogyra neglecta. The effects of initial concentration and contact time, pH, and temperature on the adsorption of lead(II) by the non-living algal biomass were studied. The equilibrium isotherms and kinetics were …

Go to article

Estimation Of The Product Yield And Collection Efficiency Of Peripheral Blood Stem Cell Harvesting Using Peripheral Blood Cd34[+] Cells Quantification., Habsah Aziz Jan 2010

Estimation Of The Product Yield And Collection Efficiency Of Peripheral Blood Stem Cell Harvesting Using Peripheral Blood Cd34[+] Cells Quantification., Habsah Aziz

Student Works (2010-2019)

Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study …

Go to article

Genotype And Allele Frequencies Of Nine Str Loci In Sikhs From Malaysia., Tan Chin Teck Jan 2010

Genotype And Allele Frequencies Of Nine Str Loci In Sikhs From Malaysia., Tan Chin Teck

Student Works (2010-2019)

Allele frequencies for nine short tandem repeats (STRs) gene loci (CSF1PO, TPOX,TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317) were obtained from analyses of saliva DNA from 109 unrelated healthy Sikh individuals residing in Malaysia. DNA was extracted using phenol/chloroform/isoamly alcohol method. 2ng of DNA for each sample was amplified following the instruction in GenePrint® STR Systems (Promega Corporation) CTT, FFv and SilverSTR III Multiplex kits. PCR products were separated in denaturing polyacrylamide gel electrophoresis and silver stained using Promega DNA Silver Staining System. The results were then calculated and processed to establish a statistical database for Sikh community. Chi-square test …

Go to article

First Prev Next Last