Digital Commons Network^™

Purification Process Development Of Protein Subunit Based Vaccine Candidates Produced Using Recombinant E. Coli Expression System (Part - I), Davinder Chawla, Yan-Ping Yang

Vaccine Technology III

Proteins are key components of prophylactic vaccines against infectious diseases. Protein subunit based vaccine is an attractive alternative to the traditional detoxified bacterial or inactivated viral vaccine approach due to its highly purified and well characterized product nature. Purification of protein antigens to achieve consistent product purity and quality is an integral part of the protein subunit vaccine product development process. Expression levels of the recombinant proteins in bacterial expression system may be extremely high following rapid technology advancement. The challenges and approaches used to develop purification processes for novel protein vaccine candidates expressed at g/L level will be discussed.

Purification Process Development Of Protein Subunit Based Vaccine Candidates Produced Using Recombinant E. Coli Expression System (Part - Ii), Davinder Chawla, Yan-Ping Yang

Vaccine Technology III

Proteins are key components of prophylactic vaccines against infectious diseases. Protein subunit based vaccine is an attractive alternative to the traditional detoxified bacterial or inactivated viral vaccine approach due to its highly purified and well characterized product nature. Purification of protein antigens to achieve consistent product purity and quality is an integral part of the protein subunit vaccine product development process. Expression levels of the recombinant proteins in bacterial expression system may be extremely high following rapid technology advancement. The challenges and approaches used to develop purification processes for novel protein vaccine candidates expressed at g/L level will be discussed.

Final Conference Program, Barry Buckland, John Aunins, Paula Marques Alves, Kathrin Jansen

Vaccine Technology III

List of talks during the conference.

Development Of Recombinant Protein Based Chemical Conjugate Malaria Vaccines Targeting The Pre-Erythrocytic Stage, Transmission Blocking, Or Both, David L. Narum, Nicholas Macdonald, David Jones, Ruth Ellis, Yimin Wu, Patrick E. Duffy

Vaccine Technology III

The development of a Plasmodium falciparum malaria vaccine is critical for future control and elimination programs. Recombinant protein based chemical conjugate vaccines, covering different parasite stages, are being developed due to complexity of the parasite and sub-optimal immunogenicity of recombinant malaria proteins in humans, respectively. Chemical conjugation of recombinant malaria proteins to carrier proteins improves their immunogenicity in animal studies. A transmission blocking vaccine comprised of the ookinete protein Pfs25 chemically conjugated to Pseudomonas aeruginosa ExoProtein A (EPA) is currently being developed for pilot scale cGMP production. Bulk lots of Pfs25 and EPA have already been produced and released following …

Scale-Up Of An Intensified Process For Rad35 Adenovirus Production Using The Per.C6® Cell Substrate, Alfred Luitjens, Herman Van Herk

Vaccine Technology III

Tuberculosis is the world’s second deadliest infectious disease, with over 9 million new cases diagnosed in 2006. In collaboration with the Aeras Global Tuberculosis Vaccine Foundation, we are developing a recombinant tuberculosis adenovirus based vaccine (rAd35). Currently a series of three Phase I trials are taking place, with the first two studies indicating very promising results, safety and toleration. In November 2008 we started a Phase II study in South Africa. The manufacturing process supporting these clinical studies was developed using the PER.C6® cell substrate. With the productivity of this production process, scale-up to 10,000-liter bioreactor will be required to …

Commencement, Philadelphia (2010), Philadelphia College Of Osteopathic Medicine

Commencements

Philadelphia Campus Commencement program, 119th class. Degrees include Doctor of Osteopathic Medicine

Can Daytime Use Of Bed Nets Not Treated With Insecticide Reduce The Risk Of Dengue Hemorrhagic Fever Among Children In Vietnam?, Ataru Tsuzuki, Vu Dinh Thiem, Motoi Suzuki, Hideki Yanai, Toru Matsubayashi, Lay-Myint Yoshida, Le Huu Tho, Truong Tan Minh, Dang Duc Anh, Paul E. Kilgore, Masahiro Takagi, Koya Ariyoshi

Department of Pharmacy Practice

The purpose of this study was to investigate the prevalence of bed net use and elucidate the effect of daytime bed net use on preventing dengue hemorrhagic fever (DHF) among children in Vietnam. We conducted a population-based cross-sectional survey and a matched case–control study in Khanh Hoa Province where not only some pre-schoolchildren but also some school children, who take a nap during lunch break prior to returning to school, used bed nets during the day. Among 36,901 children 2–10 years of age, most used untreated bed nets during the night (98.3%) compared with 8.4% during the day. The results …

Spectator 2010-06-02, Editors Of The Spectator

The Spectator

No abstract provided.

A Functional Calcium-Transporting Atpase Encoded By Chlorella Viruses, Maria Cristina Bonza, Holger Martin, Ming Kang, Gentry L. Lewis, Timo Greiner, Sonia Giacometti, James L. Van Etten, Maria Ida De Michelis, Gerhard Thiel, Anna Moroni

James Van Etten Publications

Calcium-transporting ATPases (Ca²⁺ pumps) are major players in maintaining calcium homeostasis in the cell and have been detected in all cellular organisms. Here, we report the identification of two putative Ca²⁺ pumps, M535L and C785L, encoded by chlorella viruses MT325 and AR158, respectively, and the functional characterization of M535L. Phylogenetic and sequence analyses place the viral proteins in group IIB of P-type ATPases even though they lack a typical feature of this class, a calmodulin-binding domain. A Ca²⁺ pump gene is present in 45 of 47 viruses tested and is transcribed during virus infection. Complementation analysis of …

Mitochondria-Centric Activation Induced Cell Death Of Cytolytic T Lymphocytes And Its Implications For Cancer Immunotherapy, Arvind Chhabra

UCHC Articles - Research

Premature death of the adoptively transferred cytolytic T lymphocytes (CTL) by means of activation induced cell death (AICD) represents one of the major constraints in devising an effective anti-cancer immune intervention strategy. Understanding the mechanism of AICD is, therefore, critical for developing methods to interfere with this death process. Although the existing paradigm on AICD centers around the initiation of the cascade of events originating from the engagement of death receptors leading to the activation of effector caspases and eventually resulting in cell death, recent findings have questioned the universal role of caspases as the cell death executioners. We here …

Recognizing And Managing Common Crop Disease In South Dakota, Martin A. Draper, Darrel Deneke

SDSU Extension Extra Archives

Diseases of plants may be expressed differently under varying environmental conditions or in various host species. Disease is the outward expression of an infection, yet in some cases there may be no outward evidence of disease. Outward expression may be some deviation from normal growth, change in appearance, or alteration of the quality or value of the crop. Plant diseases may be caused by biotic agents, infectious microorganisms, or abiotic causes such as chemical deposition, nutrient imbalances, or environmental factors. This publication concentrates on biotic or infectious diseases and provides information on common abiotic problems. Infectious plant diseases may be …

Spirituality As A Life Line: Women Living With Hiv/Aids And The Role Of Spirituality In Their Support System, Jennifer L. Peterson, Malynnda A. Johnson, Kelly E. Tenzek

Journal of Interdisciplinary Feminist Thought

For many women living with HIV/AIDS, incorporating spirituality into their lives helps them organize their experience, empower self-reconstruction, and manage stigma. (Stanley, 1999). Because of the potential relationship of spirituality and social support, the specific aim of this study is to examine the role of spirituality in the support experiences of women living with HIV or AIDS. In this case, spirituality or a connection to God, offered the women an opportunity to develop meaning and perspective taking, to have a source of support, to provide control through a more powerful being, and to offer a path to community.

Sizing Up Pharmacotherapy For Obesity., Michael A. Valentino, Andre Terzic, Scott A. Waldman

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Obesity has increased over the last 20 years, from a condition affecting only a small portion of populations in developed countries, into a global pandemic. The impact of obesity can be appreciated in the context of the populations at risk, and it is estimated that >1 billion adults worldwide are overweight (BMI >25 kg/m2), 300 million of whom are clinically obese (BMI >30 kg/m2). In the United States, 65% of adults are overweight, and 32.2% of them are obese, a prevalence that has doubled over 20 years. In industrialized countries, obesity rates have tripled, coinciding with adoption of a Western …

Two-Sided Exact Tests And Matching Confidence Intervals For Discrete Data, Michael P. Fay

The R Journal

There is an inherent relationship between two-sided hypothesis tests and confidence intervals. A series of two-sided hypothesis tests may be inverted to obtain the matching 100(1-)% confidence interval defined as the smallest interval that contains all point null parameter values that would not be rejected at the α level. Unfortunately, for discrete data there are several different ways of defining two-sided exact tests and the most commonly used two sided exact tests are defined one way, while the most commonly used exact confidence intervals are inversions of tests defined another way. This can lead to inconsistencies where the exact test …

What Leona Helmsley Can Teach Us About The Charitable Deduction, Ray D. Madoff

Chicago-Kent Law Review

Leona Helmsley named a number of beneficiaries under her will (both human and canine), but among the unnamed beneficiaries are scholars interested in studying the role of philanthropy in the United States. By directing that an estimated $8 billion be used for the benefit of dogs, Mrs. Helmsley brought in to high relief policy issues regarding the appropriateness of the unlimited charitable deduction. I argue that these concerns are equally applicable, albeit less obvious, when it comes to more traditional charitable bequests. In this paper I will discuss the appropriateness of the unlimited estate tax deduction (particularly in light of …

Organizing Competition And Cooperation After American Needle, Randal C. Picker

Coase-Sandor Institute for Law & Economics Research Paper Series

No abstract provided.

Biospeologica Bibliographia Publications 2010-1, Jean-Pierre Besson, Bernard Lebreton

KIP Articles

No abstract provided.

Labrad : Vol 35, Issue 2 - June 2010, Aga Khan University Hospital, Karachi

LABRAD - Departments of Pathology & Laboratory Medicine and Radiology Archives

• The Atypical Lymphocytes
• High Performance Liquid Chromatography (HPLC): An Effective Tool for the Diagnosis of Various Hemoglobinopathies
• Platelet Transfusions: What We Need to Know
• Measuring Homocysteine Levels
• Parathyroid Hormone: A Marker of Vitamin D Deficiency and Insufficiency
• Surrogate Measures of Insulin Sensitivity
• Foetal Autopsy
• Laboratory Diagnosis of Vaginitis due to Trichomonas Vaginalis
• AKU Clinical Laboratory Continuing Medical Education Seminars

Antimicrobial Activities Of Some Euphorbiaceae Plants Used In The Traditional Medicine Of Akwa Ibom State Of Nigeria, A. Essiett Uduak, K. Ajibesin Kola’

Ethnobotanical Leaflets

Nine plant species belonging to the Euphorbiaceae family and used in traditional medicine in Akwa Ibom State of Nigeria were evaluated for in vitro antimicrobial activity using agar diffusion method. The stem bark of Maesobotrya dusenii gave the most significant effect followed by its root bark. The inhibitory effect of M. dusenii stem bark extract (37 mm) on Pseudomonas aeruginosa was higher than that of Chloramphenicol (35 mm). However, Alchornea laxiflora leaf extract showed the weakest activity. The minimum inhibitory concentration of the extracts ranged between 12.5 and 250 μg/mL. The results of the antimicrobial effects validated the use of …

Christians, The Care Of Creation, And Global Climate Change (Book Review), Delmar Vander Zee

Pro Rege

Reviewed Title: Scott, Lindy (ed). Christians, the Care of Creation, and Global Climate Change. Eugene, OR: Wipf and Stock Publishers, 2008. 144pp. ISBN 13:978-1-55635-844-9.

The Effects Of Public Infrastructure And Household Characteristics On Inequality And Infant Mortality In Mexico: What Has Changed Between 1990 And 2005?, Richard S. Meindl, Jose Antonio Roldan Amaro, Cynthia Thompson, Tremaine L. Gregory, Marcela Alvarez Izazaga, Gabriel Saucedo Arteaga

Peer Reviewed Articles

The reduction of infant mortality (IM) continues to be a primary goal throughout much of the developing world, and a suite of methods have been proposed with which to achieve it. However, most attempts at IM reduction have taken place within the context of an almost bewilderingly complex regimen of simultaneous changes occurring in the physical environment, sociopolitical structure, and health care. Which of these various elements is truly causal in reducing IM and which may be merely a correlated but nonprimary agent? During the past two decades Mexico has constituted an extraordinary field laboratory with which to decipher the …

Commencement Program 2010, Loma Linda University

Commencement Programs

CONTENTS

1 | Message from the President

3 | 2010 Events of Commencement

4 | The Academic Procession

6 | Significance of Academic Regalia

8 | The Good Samaritan

9 | University History Highlights

11 | Loma Linda University Song - "Healing Love"

12 | The Speakers

22 | The University Honorees

36 | The School Honorees

59 | The Programs

• School of Medicine, 60
• School of Pharmacy, 77
• School of Dentistry, 83
• School of Science and Technology and School of Religion, 99
• School of Nursing, 112
• School of Allied Health Professions - Physical Therapy, 121
• School of Allied Health …

“‘Not In Canada’: The Non-Ebola Panic And Media Misrepresentation Of The Black Community”, Charles T. Adeyanju, Temitope Oriola

African Journal of Criminology and Justice Studies

This paper investigates the case of a non-Ebola panic in Hamilton, Ontario in 2001. Using in-depth interviews of journalists, hospital staff, members of the Congolese-Black community and content analysis of coverage by four Canadian newspapers, this study shows that the media problematized the case by cross-articulating a health scare of Ebola with immigration, crime and “race”. Utilizing extant “late modernity” literature and its attendant anxiety over risks, the study shows how a “scapegoat” was created, feasted upon by the media and aroused the collective conscience of mainstream Canadian society. Consequently, the voice of the community was misrepresented and underrepresented in …

Atención Hospitalaria De La Leishmaniosis Tegumentaria Americana En Los Miembros Inferiores, Pedro Navarro, Linda Lugo, María Antonia De La Parte, Luis Alfonso Colmenares Suárez, Nathalie Chacon, Amando Martin, Sylvia Silva, Jorge Schuller, Eduardo Salas, Adayza Figueredo

Physician Assistant Studies Faculty Publications

La leishmaniosis tegumentaria americana (LTA) como enfermedad endémica crónica continúa afectando a la población del medio rural venezolano, ocasionándole morbilidad y limitaciones laborales. Mediante la descripción de casos clínicos se evaluaron 38 pacientes con LTA que recibieron atención hospitalaria por úlceras leishmánicas en miembros inferiores. De los pacientes descritos 63 % eran niños, 84 % procedía del Estado Miranda, 66 % tenía una lesión ulcerosa única, 45 % recibió cefadroxilo previo al tratamiento antiparasitario específico y el 24 % había recibido inmunoterapia previa para la enfermedad. Treinta y siete pacientes (97 %) recibieron antimoniato de meglumina en series terapéuticas de …

Annual Report Of Undergraduate Research Fellows, August 2009 To May 2010, Office Of Research And Sponsored Programs. Morehead State University.

Office of Research and Sponsored Programs Reports and Publications

Annual Report of Undergraduate Research Fellows from August 2009 to May 2010.

Antimicrobial Activity Of Selected Fungi Isolated From Dee And Barrientos Islands, Antarctica., Shamia Mohamedain Abd Elgadir Younis

Student Works (2010-2019)

This study reports the screening of selected fungi isolated from Dee and Barrientos Islands, Antarctica, for their antimicrobial activities using two methods: a) plug assay method and b) disk diffusion method. The plug assay was carried out as preliminary screening forbiological activity against bacteria: Bacillus subtilis, Staphylococcus aureus,Escherichia coli, pseudomonas aeruginosa, and yeast: Candida albicans. The results showed that 35% from species possessed antibacterial activity; and these included: Geomyces sp.50-1/S7, Unidentified 75-1/S1, Geomyces sp.12-1/S21, Penecillium sp.75-1/S10, Geomyces sp.3-1/S5, Geomyces sp.3-4/S5 and Geomyces sp.146/S5. Most of the strains exhibited antibacterial activity against Gram-positive bacteria S. aureus and B. subtilis …

Therapeutic Modalities And Educational Approaches In Clergy Stress Reduction, Shaun Tress Campbell

Loma Linda University Electronic Theses, Dissertations & Projects

Clergy are listed among administrators, social workers, healthcare providers, fire fighters, and police officers as professionals with high levels of occupational stress (Hill et al., 2003; Greenberg, 1990). Clergy are valuable resources to the American culture in that they perform an array of services to comfort and assist others. From natural catastrophes to personal misfortune, pastors are often expected to be present to offer consolation or to extend ecclesiastical services. Researchers have found that pastors are the primary mental health counselors for tens of millions of Americans (Weaver et al., 2002). Darling et al. (2004) report, "Clergy in U.S. culture …

Global Warming: Myths & Realities, Dennis Jaynes

Social Sciences

The topic of Global Warming has been surrounded with controversy since it was first proposed. From evidence that has come from years of studies, a majority of the world’s governments and scientists are in agreement that the Earth’s global temperatures are warming. However, there are others who are skeptical of the science; they spread their message by trying to discredit the scientists or have even gone as far as to cover it up. The intent of this paper is to examine the myths and compare them side by side with the latest scientific data to determine what is real and …

Poisonous Rangeland Plants In San Luis Obispo County, Sara Litten, Amanda Ou

Animal Science

Poisonous Rangeland Plants in San Luis Obispo County is a comprehensive educational guide to rangeland plants that are toxic to domestic livestock. This guide begins with an exploration of how the biological systems are affected by the poisonous plant toxins. The biochemistry behind these toxins is included in the discussion. Next, reference material for fourteen plants that inhabit San Luis Obispo County is provided. This information includes specific toxins found in poisonous plants, affected animals, symptoms of poisoning, stages of growth, lethal dose, and distribution of the plant in California. This section of the guide is filled with helpful photos …

Influence Of Type 2 Bovine Viral Diarrhea Virus NPro On Enhancement Of Bovine Respiratory Syncytial Virus Replication Mediated By Antagonism Of Host Cell Interferon Type I Responses, Abdulrahman Abdulaziz A. Alkheraif

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, Family Flaviviridae. The virus can infect many species of animals of the order Artiodactyla. The BVDV genome encodes an auto protease, N^pro, that degrades interferon regulatory factor-3 (IRF-3) reducing type I interferon (IFN-I) production from host cells. Bovine respiratory syncytial virus (BRSV) is a member of the genus Pneumovirus, Family Paramyxoviridae. Concurrent infection with BVDV and BRSV causes more severe respiratory and enteric disease than infection with either virus alone. Our hypothesis was that N^pro modulates the innate immune responses to BVDV infection …