Digital Commons Network^™

Molecular Mechanisms Of Hepatic Insulin Resistance Following Injury, Shaoning Jiang

All ETDs from UAB

Insulin resistance commonly occurs following injuries or critical illness independent of previous diabetic status. The development of insulin resistance and hyperglycemia is associated with increased mortality and morbidity in critically ill patients. However, the molecular mechanisms underlying the acute insulin resistance following injuries remain poorly understood. With an animal model of trauma and hemorrhage, we have previously demonstrated the rapid development of insulin resistance in liver, and a critical role of TNFα in hemorrhage-induced defects in insulin signaling following resuscitation. However, hepat-ic insulin signaling is impaired prior to a significant increase in plasma TNFα, suggesting the initial development of hemorrhage-induced …

In Vitro Elucidation Of The Role And Mechanism Of Rankl In Tnf-And Il-1-Mediated Osteoclast Formation And Function, Joel Jules

All ETDs from UAB

The receptor activator of NF-қB (RANK) ligand (RANKL) and its receptor RANK play a critical role in osteoclast biology. RANK has three tumor necrosis factor receptor associated factor (TRAF)-binding motifs [PFQEP369-373 (Motif 1), PVQEET559-564 (Motif 2), and PVQEQG604-609 (Motif 3)] that regulate osteoclast formation and function. RANK also contains a TRAF-independent motif (IVVY535-538) that commits bone marrow macrophages (BMMs), which are osteoclast precursors, to the osteoclast lineage for osteoclastogenesis. Notably, tumor necrosis factor-α (TNF) and interlukin-1 (IL-1) utilize TRAFs to initiate most of the signaling pathways known to be activated by RANKL but fail to form osteoclasts unless attended by …

The Role Of The Intracellular Signaling Pathway In Ehrlichia Canis Infection In Vitro, Chang-Hyun Kim

All ETDs from UAB

Ehrlichia canis is a pleomorphic obligatory intracellular gram-negative bacterium that causes tick-borne canine ehrlichiosis. A systematic investigation on the pathogenesis of the disease has been hindered largely by lack of a reliable quantitative assay. Using quantitative real time PCR (qPCR), a quantitative assay for E. canis was developed and validated. Using the qPCR, the intracellular proliferation kinetics of E. canis in the canine 030F cell-line was studied for the first time. Many intracellular bacterial pathogens have been shown to use Ca2+ signaling in the host cells to induce their own internalization and proliferation. However, it is not clear how Ca2+ …

Identification And Characterization Of Downstream Target Genes Of The Bmp Signaling Pathway, Nam Chul Kim

All ETDs from UAB

During nervous system development, neurons proliferate, differentiate, project their axons to their targets and make synapses with them. At the neuromuscular junction of Drosophila, these functional synapses enlarge as the body grows to maintain physiologically effective synaptic transmission with the muscles. Drosophila Bone Morphogenetic Protein (BMP) type II receptor, Wishful thinking (Wit), is important in this proper synaptic growth and function. To identify transcriptional targets of Wit signaling, we performed microarray experiments comparing normal controls to wit mutants. Through this study, we found that some of the verified target genes showed isoform-specific regulation in wit mutants, and that several neuropeptide …

Spin Echo Ss-Parse: A Parse Mri Method To Estimate R2, R2' And Frequency In A Single Shot, Ningzhi Li

All ETDs from UAB

Rapid and accurate mapping of relaxation rates applies to a number of neuroimaging and functional magnetic resonance imaging (fMRI) studies. Previously developed relaxation rate mapping methods are based mostly on non-linear fitting of intensity images. For the first time, this study developed an ultrafast and direct transverse relaxation rate mapping technique, Spin Echo Single-Shot Parameter Assessment by Retrieval from Single Encoding (SE-SS-PARSE). Four useful imaging parameters, local transverse magnetization magnitude (Mxy0), frequency (f), reversible and irreversible transverse re-laxation rate (R2' and R2), can be estimated using an iterative searching algorithm simul-taneously and quantitatively. In addition, the SE-SS-PARSE technique is free …

Reading Achievement In An Urban School District, Kathleen W. Lindsey

All ETDs from UAB

READING ACHIEVEMENT IN AN URBAN SCHOOL DISTRICT KATHLEEN W. LINDSEY EDUCATIONAL LEADERSHIP ABSTRACT The purpose of this study was to determine if differences in elementary reading comprehension achievement existed as a result of statewide implementation of the Alabama Reading Initiative post No Child Left Behind (NCLB). Research supports the premise that targeted instruction in reading comprehension yields increases in reading achievement for students. In an effort to increase students' reading comprehension, the Alabama State Department of Education embarked upon a bold initiative targeting all areas of reading. The Alabama Reading Initiative (ARI) was designed to address K-12 reading efforts in …

Role Of 14-3-3&Tau In Autophagy And Role Of Edd In P53 Regulation, Shiyun Ling

All ETDs from UAB

Unrestricted cell proliferation and suppression of cell death are two essential events for tumor development. My dissertation research involves two proteins, 14-3-3 &tau and EDD which are involved in diverse pathways related to these two fields in recent studies. Previous study demonstrates that 14-3-3ô regulates p21 degradation. Up-regulation of 14-3-3ô is seen in breast cancer and is correlated with the down-regulation of p21 in breast cancer. Amplification or overexpression of EDD was observed in breast cancer and ovarian cancers. Illustrating the new roles of these two proteins in proliferation and cell death will advance our knowledge in tumorigenesis and help …

Identification And Characterization Of Virulence Factors Of Mycoplasmas, Wenyi Luo

All ETDs from UAB

Mycoplasmas cause a group of diseases that are characterized by their chronicity and resistance to treatment. Diseases caused by the murine pathogens Mycoplasma pulmonis and Mycoplasma arthritidis are often studied as models of human diseases caused by a variety of chronic pathogens that induce a similar pathology. Among several candidate pathogenic factors, superantigens and degradative enzymes such as glycosidases are potentially important but not well characterized. The M. arthritidis mitogen (MAM) is a superantigen secreted by M. arthritidis. Its role with respect to arthritogenicity and toxicity is unclear. To improve the efficiency of transformation and hence enhance efforts to generate …

Lim Kinase 1 And Twinstar In Synaptic Plasticity Of Drosophila Motoneurons, James Brian Machamer

All ETDs from UAB

Modification of the actin cytoskeleton is essential for synaptic plasticity, which is believed to underlie the brain's capacity for learning and memory. Regulation of the actin cytoskeleton by LIM Kinases (LIMK1 and LIMK2) and their downstream targets Actin Depolymerization Factor/Cofilin (ADF/Cofilin) in the mouse forebrain modulates dendritic spine morphology, synaptic function, long-term potentiation (LTP), and learning and memory, but appear dispensable for the regulation of most forms of presynaptic plasticity. However, these analyses were performed in mice mutant for single members of these gene families, and thus the interpretation of these results is confounded by potential compensatory activity of homologous …

Translocation And Function Of Akt In The Mitochondria, Keri A. Mans

All ETDs from UAB

The ubiquitously expressed kinase Akt is a known survival protein, and is involved in multiple cell signaling cascades, notably the phosphatidylinositol 3-kinase (PI3K) pathway. Active Akt translocates from the plasma membrane to different subcellular compartments, including mitochondria, where it phosphorylates compartment-specific substrates. The mechanism of this translocation and the specific function of Akt within the mitochondria remains a mystery. The goals of this study were to elucidate the mechanism by which Akt enters the mitochondria and examine the role of Akt in mitochondrial function. Finally, the possibilities of using post-mortem human brain tissue to study mitochondrial function in normal and …

Translation Initiation Signaling Components Altered By Mechanical Load Dictate Skeletal Muscle Hypertrophy, David Lawrence Mayhew

All ETDs from UAB

The regulation of protein synthesis (i.e., mRNA translation) is an energetically costly and extensively regulated process, which is primarily regulated at the initiation step. Mechanical load is a potent stimulus of translation in skeletal muscle, and thus this tissue provides an excellent model system to study the plasticity of the translational apparatus. We investigated the effects of alterations in translation initiation cell signaling pathways in skeletal muscle using a variety of in vitro and in vivo mouse and human model systems. Collectively, our results suggest that, although many proteins in translational signaling pathways are responsive to mechanical load, the response …

The Environmental And Linguistic Linkages Between Poverty And Education, Susan Mccarroll

All ETDs from UAB

Statistically, children who live in poverty are unfairly plagued primarily with a life of educational deficits. Researchers are constantly studying this phenomenon and have attributed several factors to the decrease in quality of life seen by impoverished children. For example, quality of the physical environment, of parental care, and of school systems all can play a large role in the intellectual ability of a child living in poverty. Moreover, children who have lower exposure to toxins, such as lead, have higher IQs and better reading ability than children who have had over-abundant exposure to negative environmental factors. Nationally, early education …

A Comparative Study Of Radiological Threat Environments And Radiation Control, Lisa C. Mccormick

All ETDs from UAB

Radiation Control Programs (RCPs) differ from state to state as does their integration with public health agencies. The purpose of this study was threefold: to describe and compare across states the environmental radiological attributes of RCPs, to describe how these programs are organized structurally within state government, and to identify if RCPs differ structurally in environments of differing radiological attributes. Publicly available information from the Nuclear Regulatory Commission and state agency websites was used to determine both the environmental attributes and the different approaches states use to organize RCPs. States were grouped based on environmental attributes by employing a principle …

What It Means For Grandparents To Raise Their Grandchildren As Primary Caregivers, Annette I. Mohan

All ETDs from UAB

ABSTRACT For generations, grandparents have taken a special role in the lives of their grandchildren- to nurture, love, encourage and support them to achieve their goals. However, in the past fifteen years, the definition of family has become more flexible, as it accommodated the increasing number of grandparents raising grandchildren as primary caregivers. This research studies this family structure, which was seen as a solution to raise young children whose parents were unfit to perform their role as parents or because of a crisis. Grandparents who raise their grandchildren as primary caregivers were chosen using purposeful snowball sampling. The grandchildren …

Genotyping Of Streptococcus Mutans Using Multilocus Sequence Typing, Stephanie Momeni

All ETDs from UAB

ABSTRACT The role of Streptococcus mutans has been extensively studied using a variety of genotyping techniques. Repetitive extragenic palindromic polymerase chain reaction (rep-PCR) is an effective tool for screening large-scale epidemiological studies with high discriminatory power and reproducibility. For this study, multilocus sequence typing (MLST) analysis is used to evaluate genotypes previously identified as unique using rep-PCR. Twenty-two unique S. mutans rep- PCR genotypes were selected from a longitudinal study. Four additional isolates were selected from each genotype group of the 6 most commonly occurring genotypes (n=30) for further analysis. Real-time PCR was performed using eight housekeeping S. mutans gene …

The Role Of Parental Autonomy Support In The Motivation Of The Health Behaviors Of Diet And Exercise In Older Adolescents, Shannon A. Morrison

All ETDs from UAB

regardless of the degree of heteroskedasticity. Moreover, in the presence of high leverage

Twenty Five Years Of Surgical Odyssey - 1985 - 2010, Dr. Muneer Amanullah

Department of Surgery Archives

• 25 Years: Just the First Step of a Long Journey Editor's Foreword
• Deans Message
• A Rewarding Journey
• The Journey Ahead
• First Chairperson's Message
• A Futuristic Tomosynthesis
• Snippets, On Reviewing 28 Years in an Organization
• Departmental 0rganogram
• Brief Profiles of Past and Present Department Professors
• Sectional Photographs
• Our Volumes Over the Years
• SURGERY ALUMNI - RESIDENCY & FELLOWSHIP PROGRAMS
• List Of Residents' Submitted CPSP Dissertations (FCPS-II)
• Publish Or Perish: A Compilation Of 25 Years of Department's Published Research
• Analysis Of 2 5 Years of Published Research
• Research Grants Received by The Department
• Surgical Grand Rounds: What Happens After the Cake and …

Effects Of Caregiving Strain On All-Cause Mortality, Martinique Perkins

All ETDs from UAB

Purpose. This study examined how perceived caregiving strain is related to quality of life, mental and emotional health, and mortality in a large, national sample. Methods. We used 3,714 caregivers from the REasons for Geographic and Racial Differences in Stroke (REGARDS) study. Participants had to complete baseline, in-home visit, and follow-up interviews to be included. Results. Approximately 12% of the REGARDS sample reported that they provided on-going care to a family member with chronic illness or a disability. Highly strained caregivers had the worst mental, physical, and emotional health but there was evidence of increased social support lessening the relationships …

The Contribution Of Different Mechanisms Of Viral Sequence Variation To The Evolution Of Positive-Sense Single-Stranded Rna Viruses, Brett Earl Pickett

All ETDs from UAB

The Flaviviridae family of positive-sense single-stranded RNA (+ssRNA) viruses includes viral taxa which greatly impact public health worldwide. To explore how the viruses within the Flaviviridae family evolve, we examined the extent to which these viral taxa use nucleotide covariance, spontaneous mutation, and/or homologous recombination to vary their genotype as well as the resulting phenotype. We developed and used CovarView to assist us in simultaneously viewing and inspecting the results from whole genome covariance analyses. This resulted in the identification of previously-characterized RNA functional structures in the genomes of hepatitis C virus (HCV), as well as a new RNA functional …

Regulation Of Glycogen Synthase Kinase 3 In The Pathophysiology And Treatment Of Mood Disorders, Abigail Marie Polter

All ETDs from UAB

Mood disorders are devastating psychiatric illnesses that will affect as many as one in every five persons worldwide over the course of their lifetime. Significant gaps still remain in our understanding of these illnesses and outcomes for many patients are far from optimal. Glycogen Synthase Kinase 3 (GSK3) is a protein kinase that is increasingly recognized as playing an important role in a number of neuronal functions, as well as in pathological states such as mood disorders. GSK3 is inhibited in the brain by phosphorylation on an N-terminal serine. Several treatments for mood disorders, including lithium, antidepressants, and atypical antipsychotics …

Assessing Knowledge, Attitudes, And Beliefs Of Teachers In Alabama Concerning Comprehensive Hiv Education, Steven Brent Powell

All ETDs from UAB

The Alabama State Department of Education has guidelines for educators to insure HIV information is taught by teachers starting in fifth grade (Alabama Course of Study: Health Education, 2009). As assessed by The School Health Profile (Profiles), 55% of Alabama teachers received professional development on HIV/AIDS. In addition, less than half of grades 9-12 teachers taught how condoms work, the importance of consistent condom use, and how to obtain condoms (U.S. Department of Health and Human Services, 2009). The Youth Risk Behavior Surveillance Survey (YRBSS) administered to grades 9-12 determined that 57% of youth in Alabama are sexually active, with …

Cognitive-Behavioral Intervention To Enhance Communication Skills For Cancer Patients, Heather Prayor-Patterson

All ETDs from UAB

Introduction: With nearly 12 million Americans living with or having a history of cancer, the patient-doctor communication process is especially important for this population. Cancer patients are often dissatisfied with the quality of the information they receive regarding their treatment, side effects, symptom control, and the quality of the relationship with their healthcare provider. A patient's self-efficacy during patient-doctor interactions influences the level of trust in his/her doctor, satisfaction with care, and the type of information exchanged. Studies have focused on intervening in patient communication, but there are limitations in the literature, including a focus on improving self-efficacy, addressing communication …

Exploring The Effects Of Limited English Language Proficiency On Disability: A Pilot Study Of Adult Chinese-, Japanese-, And Korean-Language Speakers In The United States, Xiaofei Qiao

All ETDs from UAB

This is a pilot study contending that limited language proficiency is associated with disability outcomes for Chinese-, Japanese-, and Korean-speaking Americans in the United States, as determined by application of the Disablement Process model. Using the 5 percent public-use micro-data samples (PUMS) from the United States Census 2000, this study explores the relationship by employing a Poisson regression on these three language groups from a comparative perspective. The findings shed light on the significant effects of limited language proficiency as a predisposing risk factor on disability outcomes and also demonstrate the importance of individual, family, and community characteristics as either …

Response Dynamics Of Retinal Ganglion And Early Visual Cortical Cells To Simulated Optical Blur, Michael Lance Risner

All ETDs from UAB

Visual neurons are highly sensitive to changes in luminance around the mean level of light. The effects of changes in luminance contrast on the responses of retinal ganglion cells (RGCs) and V1 neurons have been extensively studied. Sharp transitions in luminance signify edges, which are key features in the visual environment. However, edges are rarely sharp and may be blurred. Edges are generally blurred because eyes have a restricted depth of field, sources of light are rarely point sources, edges are typically smooth, and rapid motion induces blur. The purpose of this research was to understand how RGCs and V1 …

Heparanase Drives The Aggressive Myeloma Phenotype: Preclinical Development Of A Heparanase Inhibitor For The Treatment Of Multiple Myeloma, Joe Ritchie

All ETDs from UAB

Heparanase, an endoglycosidase which cleaves heparan sulfate chains at specific sites, is rarely expressed in normal tissues but becomes evident in many human cancers. We have previously shown that heparanase promotes myeloma growth and angiogenesis through modulation of the tumor microenvironment. Recognition that heparanase drives the aggressive myeloma phenotype has led to new strategies designed to therapeutically target this enzyme. SST0001, a non-anticoagulant heparin that is 100% N-acetylated and 25% glycol split, was previously described as a potent inhibitor of heparanase activity in vitro, and, in limited in vivo experiments, SST0001 was identified to have efficacy as an anti-tumor agent …

Principals Mentoring Teachers To Improve Instructional Practices: A Multiple Case Study, Walter Burke Wren

All ETDs from UAB

ABSTRACT The purpose of this multiple case study was to examine the mentoring relationship between principals and their teachers in regards to instructional practice at three demo-graphically diverse elementary schools. Multiple data sources were used to conduct qua-litative research which resulted in extensive data collection and analysis. The collection of data was conducted through direct observations, semi-structured interviews, and a review of documents provided by each research site. This case study used the theories of adult learning, instructional leadership and transformational leadership to explain the findings, provide focused implications for mentoring processes between principals and teachers, and to influence the …

Therapeutic Interventions For Chlorine Gas Induced Lung Injury, Amit Kumar Yadav

All ETDs from UAB

Rationale: Nitrite (NO2-) has been shown to limit injury to the heart, liver and kidneys in various models of ischemia-reperfusion injury. Currently, potential protective effects of systemic NO2- in limiting lung injury or enhancing repair have not been documented. Objectives: To assess the efficacy and mechanisms by which post-exposure intra-peritoneal injections of NO2- mitigate Cl2 induced lung injury in rats. Methods: Rats were exposed to Cl2 (400 ppm) for 30 minutes and returned to room air. Nitrite (1mg/Kg) or saline were administered intraperitoneally at 10 min, 2, 4 and 6 hrs post exposure. Rats were sacrificed at 6 hrs or …

The Role Of Map Kinase Cascade In Msp Signaling Response, Youfeng Yang

All ETDs from UAB

The MSP domain is an evolutionarily conserved immunoglobulin-like structure of about 120 amino acids (Miller et al., 2001). A P56S missense mutation in the MSP domain of the human vapb gene is associated with a dominantly inherited form of Amyotropic Lateral Sclerosis (ALS) or Spinal Muscular Atrophy (SMA) (Nishimura et al., 2004). In C. elegans, secreted MSP binds to the VAB-1 Eph receptor and other receptors on oocyte and ovarian sheath cell surfaces (Miller et al., 2003). We have recently shown that the P56S mutation prevents secretion of the VAPB MSP domain, suggesting that the signaling function is important in …

Il-21 Dictates The Quality Of The Cd8 T Cell Response During Acute And Chronic Infections, John Seung-Hoon Yi

All ETDs from UAB

CD8 T cells contribute to the control of intracellular pathogens including viruses and certain bacteria. Recognition of foreign antigens by antigen-specific CD8 T cells initiates a differentiation process by which CD8 T cells gain effector functions including the ability to rapidly produce cytokines and kill infected cells. Currently, it is not well understood what signals dictate the generation of robust CD8 T cell responses, however it has been demonstrated that without CD4 T cell help CD8 T cell responses are compromised. CD4 T cells are the primary producers of interleukin-21 (IL-21), which has broad immunological actions. Thus for this dissertation, …

Cardiovascular Endothelial Regeneration On An Electrospun Scaffold, Xing Zhang

All ETDs from UAB

A bi-layer electrospun nano-fibrous scaffold comprising synthetic polymers and natural proteins was constructed as the prototype of an arterial substitute for coronary ar-tery disease. This scaffold featured a highly porous microstructure analogous to native extracellular matrix. Uni-axial tensile test suggested that its excellent mechanical strength could restore normal blood flow immediately after implantation. Subsequent biocompati-bility study demonstrated that scaffolding materials, either traditional or degraded, were non-toxic to the regenerated human endothelium on the luminal layer. Moreover, this re-generated endothelium possessed critical functions, such as the formation of tight junc-tions, the secretion of prostacyclin, and the prevention of platelet adhesion. Thereafter, …