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Computer-Based Skeletal Age Assessment Using Hand/Wrist Radiographs In Children 8-18 Years Old, Zhihong Ni

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Children's skeletons mature at different rates, and they can be affected by a variety of factors including disease, hormone imbalance or genetics. The assessment of skeletal maturity is a frequently performed procedure that allows the detection of hormonal, growth or genetic disorders. Several methods have been developed to estimate skeletal maturity. Most methods evaluate hand/wrist radiographs using indicators such as the ratios of various bone widths, the onset of the ossification of epiphysis and epiphyseal-diaphyseal fusion. Among those methods, the FELS method differs from others in the application of different grades to each indicator and the provision of a confidence …

Tissue Specific Effects Of Adipose Stem Cells (Asc) In A Melanoma Tumor Environment, Drew Michael Nedderman

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This study determined the tissue-specific effect of adipose stem cells (ASC) within a melanoma environment for development of a cell-based melanoma therapy. Analysis included a subcutaneous B16-F1 melanoma model using thirty-one C57BL/6 wild-type mice. Melanoma xenografts were treated with cell-based therapies of CFDA-SE-labeled human fibroblasts HF20x (control), non-differentiated ndASC or hematopoietic-differentiated HdASC. No tumor regression was observed in presence of cell-based therapies, thus, the HdASC group demonstrated an increase in tumor growth accompanied with an up-regulated macrophage response, and increased angiogenesis. In addition, this group demonstrated a decrease in Melan-A tumor marker and interferon-γ expression suggesting that ASC-supported tumor angiogenesis …

Biodegradation Of Groundwater Pollutants (Chlorinated Hydrocarbons) In Vegetated Wetlands: Role Of Aerobic Microbes Naturally Associated With Roots Of Common Plants, Christina Lynn Powell

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Chlorinated aliphatic hydrocarbons (CAHs) are often found as groundwater contaminants because of past industrial activities and disposal practices. CAHs pose a threat to human health and thus, create a need to find both natural and engineered processes that can remove these chlorinated compounds from the environment. Natural attenuation by oxidative biodegradation is especially important because it can allow for mineralization to carbon dioxide, a nontoxic end-product. The goal of this research was to evaluate the potential oxidative biodegradation of CAHs by microorganisms that are naturally associated with wetland plant roots. The research was divided into field work and laboratory batch …

Charaterization Of Chlorpyrifos Toxicity On The Pancreatic Beta Cell Line Rinm5f, Zhongyu Yan

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Chlorpyrifos (CPF) is a well-known organophosphate (OP) insecticide that inhibits acetylcholinesterase (AChE). Organophosphates have been shown to induce hyperglycemia in both humans and animals that cannot be explained by AChE inhibition alone. Pancreatic β-cells are responsible for secreting insulin and playing a fundamental role in glucose homeostasis. The aim of this research was to investigate CPF toxicity on the insulin secreting β-cell line RINm5f (RIN). In the first part of this dissertation, we determined that cytotoxic concentrations of CPF between 50 µM and 200 µM induced a dose-dependent increase in RIN cell apoptotic and necrotic death (p<0.05). Lower doses, i.e., 5-25 µM did not induce apoptosis or necrosis. In further mechanistic studies, inhibitors of c-Jun N-terminal kinase (JNK) and P38 pathways, i.e., SP600125 and SB202190, successfully attenuated CPF-induced apoptosis (p<0.05), suggesting cell death is mediated by JNK and/or p38. In addition, N-Acetyl-L-cysteine (NAC), an antioxidant, inhibited apoptosis suggesting that oxidative stress is also involved in RIN cell death. In the second part of this dissertation, low dose CPF's effects on insulin synthesis and secretion was studied. Our results showed that RIN cells treated with CPF for 4 hr exhibited a dose-dependent decrease in insulin secretion in response to stimulation by 50 mM potassium (p<0.05). Insulin secretion stimulated by 25 mM glucose also showed significant decreases upon incubation with various doses of CPF (p<0.05). Insulin synthesis involves proteolytic cleavage of proinsulin at two specific sites. The enzymes responsible for promoting proinsulin cleavage, i.e, prohormone convertase (PC) 1/3 and 2 (PC2), contain a serine hydrolase catalytic triad, His-Asp-Ser, similar to AChE. CPF modification of PC enzymes similar to AChE, could potentially interrupt the conversion of proinsulin to insulin and disrupt insulin secretion. PC2 enzyme activity was significantly inhibited by CPF at concentrations as low as 10 µM (p<0.01). RIN cells, for the first time, were found to secrete proinsulin was through a constitutive instead of a regulated pathway. In summary, our results show that CPF not only exerts a direct cytotoxic effect at concentrations above 50 µM, but also significantly impairs insulin secretion at lower doses. Low dose CPF could potentially affect endocrine function in vivo by inhibiting the proinsulin processing enzyme PC2.

The Political Economy Of Affordable Housing Development In Suburban Hennepin County, Minnesota, Patrick Malloy

Geography Honors Projects

A shortage of housing affordable to low- and moderate-income households in job-rich suburbs is one of the biggest obstacles to upward mobility for those households, yet our understanding of the factors influencing the distribution of affordable housing development is weak. Focusing on suburbs in Hennepin County, Minnesota, I use qualitative case studies of eight cities and a statistical analysis of 39 to demonstrate that the geography of economic incentives to develop affordable units has the strongest influence. Contrary to common beliefs, opposition of middle-class suburbanites to affordable housing is a weak factor. Public policy should therefore focus on altering economic …

The Influence Of Stored Product Mites On The Function Of Skin Microvascular Endothelial Cells, Jennifer Graff Curp

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The prevalence of allergenic diseases and sensitization to stored product mites is increasing worldwide. Stored product mites are prevalent in homes, foods, and some work environments. Stored product mites need to be further studied so we can characterize their inflammatory properties to understand their role in skin disease, and provide proper treatment to patients with mite allergies. The purpose of the study was to show the role of stored product mites in modulating the inflammatory and immune response to human dermal endothelial cells in the skin. The epidermis serves as the first line of defense between the body and the …

A Novel Synergistic Diagnosis Methodology For Identifying Abnormalities In Wireless Capsule Endoscopy Videos, Alexandros Karargyris

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Wireless Capsule Endoscopy (WCE) is a new technology that allows medical personnel to view the gastrointestinal (GI) mucosa. It is a swallowable miniature capsule device the size of a pill that transmits thousands of screenshots of the digestive tract to a wearable receiver. When the procedure finishes the video is uploaded to a workstation for viewing. Capsule Endoscopy has been established as a tool to identify various gastrointestinal (GI) conditions, such as blood-based abnormalities, polyps, ulcers, Crohn's disease in the small intestine, where the classical endoscopy is not regularly used.

As of 2009 the market is dominated by Given Imaging …

Design And Development Of A Wireless Data Acquisition System For Fall Detection, Anoop Hanchinamane Ramakrishna

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With the mortality rate of the elderly on the rise due to fall related incidents, fall detection has become an important entity in the geriatric health care sector. The study conducted in this thesis involved the design and development of a Wireless Data Acquisition System (WDAS) with Bluetooth capabilities interfaced to pulse width modulated gyroscopic sensors. Each sensor used has a reference output pulse width which changes when rotated about its sensitive axis.Part of the effort involves the design and development of two versions of printed circuit boards for the application. Each board includes a Parallax Propeller micro- controller in …

Skeletal Pathology Of Tibiotarsi In Chick Embryos Exposed To Platinum Group Metals By Micro-Raman Spectroscopy, Jennifer L. Monahan

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Platinum group metals (PGMs) have been shown to accumulate in various tissues of organisms but their toxicity is not well-known. Raman spectroscopy is a powerful analytical technique that enables direct and non-destructive chemical characterization of bone tissues. PGMs were injected into chick embryos in the following concentrations: 0.1, 1.0, 5.0, or 10.0 ppm solutions of Pt^(IV), Rh^(III), Pd^(II) or PGMs mixtures (0.1 ppm or 1.0 ppm of each metal). The micro-Raman maps recorded on the paraffin embedded tibiotarsus cross-sections showed significant changes in the chemical composition and structure of the bone tissue as a result …

Algorithmic Techniques Employed In The Quantification And Characterization Of Nuclear Magnetic Resonance Spectroscopic Data, Paul Edward Anderson

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Nuclear magnetic resonance (NMR) based metabolomics is a developing research field with broad applicability, including the identification of biomarkers associated with pathophysiologic changes, sample classification based on the mechanism of toxicity, and clinical diagnosis. Intrinsic to these applications is the need for statistical and computational techniques to facilitate the associated data analysis. Further, a typical 1H NMR spectrum of pure proteins, biofluids, or tissue may contain thousands of resonances (i.e., peaks), thus, a pure visual inspection is insufficient to fully utilize the spectral information.

Common practice within the NMR-based metabolomics community is to evaluate and validate novel algorithms on empirical …

Semantic Provenance: Modeling, Querying, And Application In Scientific Discovery, Satya Sanket Sahoo

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Provenance metadata, describing the history or lineage of an entity, is essential for ensuring data quality, correctness of process execution, and computing trust values. Traditionally, provenance management issues have been dealt with in the context of workflow or relational database systems. However, existing provenance systems are inadequate to address the requirements of an emerging set of applications in the new eScience or Cyberinfrastructure paradigm and the Semantic Web. Provenance in these applications incorporates complex domain semantics on a large scale with a variety of uses, including accurate interpretation by software agents, trustworthy data integration, reproducibility, attribution for commercial or legal …

Crustacean Endocrine Disruption Through A Pathway Involving Nuclear Receptors, Cyclic Nucleotides And Calcium Transporters, Laxminath Tumburu

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Endocrine disruption is a complex phenomenon in the sense that endocrine disrupting chemicals (EDCs) are known to act via multiple modes of action, but the mechanisms of actions are poorly understood. In crustaceans, calcium (Ca²⁺) apart from its role as a second messenger, is also a major constituent of the calcified exoskeleton which undergoes a periodic mineralization/demineralization process known as the molting cycle. Molt cycle is under the control of steroid hormones, ecdysteroids. EDCs disrupt this molting process via their interference with receptor-mediated ecdysteroid signaling. However, the hormonal regulation of Ca²⁺ flux in crustacean molting is poorly …

Toward A Whole-Landscape Approach For Sustainable Land Use In The Tropics, R. Defries, C. Rosenzweig

United States National Aeronautics and Space Administration: Publications

Increasing food production and mitigating climate change are two primary but seemingly contradictory objectives for tropical landscapes. This special feature examines synergies and trade-offs among these objectives. Four themes emerge from the papers: the important roles of both forest and agriculture sectors for climate mitigation in tropical countries; the minor contribution from deforestation-related agricultural expansion to overall food production at global and continental scales; the opportunities for synergies between improved food production and reductions in greenhouse gas emissions through diversion of agricultural expansion to already-cleared lands, improved soil, crop, and livestock management, and agroforestry; and the need for targeted policy …

Development Of A Multi-Stage Axial Flow Cyclone, Ta-Chih Hsiao, Da-Ren Chen, Lin Li, Paul Greenberg, Kenneth W. Street

United States National Aeronautics and Space Administration: Publications

A prototype multi-stage cyclone system consisting of an impaction inlet and five axial flow cyclone stages has been developed to classify simulants of Lunar and Martian dusts for various research and development needs of NASA’s space exploration missions. Individual axial flow cyclone stages can be either independently operated with an inline connection to other particle devices or cascaded together for particle separation and collection. The impaction inlet and first three cyclone stages were designed to operate at the flowrate of 50 lpm under pressure close to ambient. The last two cyclone stages were designed to operate under low pressure conditions …

Inferring Lake Depth Using Diatom Assemblages In The Shallow, Seasonally Variable Lakes Of The Nebraska Sand Hills (Usa): Calibration, Validation, And Application Of A 69-Lake Training Set, Avery L. C. Shinneman, Danuta M. Bennett, Sherilyn C. Fritz, Jens Schmieder, Daniel R. Engstrom, Aris Efting, John Holz

Department of Earth and Atmospheric Sciences: Faculty Publications

The Nebraska Sand Hills are a distinctive eco-region in the semi-arid Great Plains of the western United States. The water table underlying the Sand Hills is part of the High Plains/ Ogallala aquifer, an important water resource for the central Great Plains. Lake levels are affected directly by fluctuations in the water table, which is recharged primarily by local precipitation and responds quickly to climatically induced changes in regional water balance. Instrumental records are available for only 50–100 years, and paleolimnological data provide important insights into the extremes and variability in moisture balance over longer time scales. A set of …

Transparency, Accountability, And Competency: An Essay On The Obama Administration, Google Government, And The Difficulties Of Securing Effective Governance Symposium: What Change Will Come: The Obama Administration And The Future Of The Administrative State, Ronald J. Krotoszynski Jr.

Articles

No abstract provided.

Predatory Bundling And The Exclusionary Standard, J. Shahar Dillbary

Articles

Recent decisions--all relying on a stylized example first provided by the Ortho court--hold that a multi-product seller that uses a bundled discount in a way that excludes an equally or more efficient competitor engages in predatory bundling. According to these decisions, a bundle can be considered “predatory” even when the price of the bundle exceeds its cost. This Article shows that the Ortho court's stylized example and its monopoly leveraging theory are erroneous. This Article further demonstrates that even when a bundle's price excludes more efficient competitors and even when a component in the bundle is priced below cost, and …

Living History Interview Comment, Richard Delgado, Jean Stefancic

Articles

No abstract provided.

Table Of Contents

Michigan Journal of Public Health

No abstract provided.

Evaluation Of Prevalence, Transmission, And Prevention Methods Of Salmonellosis From Pet Turtles In Michigan, Karina Ballester, Edwin Vazquez, Mahdi Saeed, Paul C. Bartlett

Michigan Journal of Public Health

The sale and distribution of turtles with a carapace smaller than four inches in diameter was banned by federal law in 1975 on the grounds that such animals were frequently associated with human Salmonella infections. However, the popularity of these small turtles as pets has resurfaced, and in many places in Michigan they are being sold illegally. This study was conducted in Michigan to determine the prevalence of Salmonella spp. in the most popular pet turtle species, Trachemys scripta elegans commonly known as red-eared sliders, and to evaluate the compliance of pet stores with laws concerning the sale of these …

Presynaptic Regulation Of Carotid Body Type I Cells By Histaminergic And Muscarinic Receptors, Carrie Marie Thompson

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Type I cells are one of two main cell types located within the carotid body. These cells respond to hypoxia, hypercapnia, and acidosis by releasing excitatory and inhibitory neurotransmitters. This causes increased firing of the carotid sinus nerve and restores blood gas levels to their physiological values. While previous studies have shown whether individual neurotransmitters are excitatory or inhibitory, this work demonstrates how the interplay between two neurotransmitters may potentially shape the output of the carotid body. Histamine, which has previously been shown to have no effect on intracellular Ca2+ in type I cells, may function to modulate the actions …

Fault Diagnosis Of Vehicular Electric Power Generation And Storage, Hithesh Sanjiva Uliyar

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Automotive vehicles are becoming increasingly dependent on electric power, and this trend will accelerate. The health of the vehicular electric power generation and storage (EPGS) system is crucial to enhance vehicle safety, fuel economy, and customer satisfaction.

In this research, a state-of-health (SOH) monitoring method is developed for automotive EPGS system, focusing on alternator related faults. Parity relations, characterizing the correlation among EPGS system signals under normal operating conditions, are generated using principal/minor component analysis techniques. Based on a design of structured residuals, the faults under consideration, including shorted diodes, belt slippage, and regulator fault, are successfully detected and isolated. …

The Importance Of Perceived Similarity Within Faculty-Faculty Mentoring Dyads, Emily N. Polander

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This study investigated the effects of gender similarity, perceived similarity, and relationship type (formal vs. informal) within faculty-faculty mentoring dyads on various mentoring outcomes from the protégé's perspective. Perceived similarity was expected to be a stronger predictor of relationship satisfaction, affective commitment, job satisfaction, and turnover intent than gender similarity. Perceived similarity was also examined as a potential mediator of relationship type and relationship satisfaction. Tenure-track faculty who reported having mentors (N = 45) answered questions regarding their primary career mentor and other workplace attitudes. Results indicated that perceived similarity had a positive, greater effect than gender similarity on relationship …

Domain-Specific Core Self-Evaluations And Stressors As Predictors Of Strains, Michael Raymond Hoepf

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The current study examines the use of a frame-of-reference (FOR) in the evaluation of core self-evaluations, stressors and strains. The study investigates the prediction that using a FOR that matches predictors and outcomes will result in a stronger relationship between the constructs being measured, specifically CSE, stressors, and strains. In addition, the study examines the extent to which following this procedure will add incremental validity after non-contextualized predictors are controlled. The hypotheses in this study were generally supported, although the hypotheses related to the school domain were not supported as strongly as those related to general and work domain. The …

Structural Health Monitoring Of A Thermal Protection System For Fastener Failure With A Validated Model, Randy Joseph Tobe

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Air vehicles flying at hypersonic speeds encounter extreme thermal, aerodynamic and acoustic loads. To maintain the structural integrity of the flight vehicle, a thermal protection system shields the main structure from these loads. Therefore, maintaining the health of the thermal protection system is critical for a successful mission and vehicle safety. One of the more common types of failure in a mechanically attached thermal protection system is fastener failure. Since reducing vehicle turnaround between flights is desired, creating an automated system to perform structural health monitoring on the fastener health of the thermal protection system is needed. This can be …

Cellular Effects Of Replicating A Polypurine-Polypyrimidine Sequence And The Interactions Of Due-B With Replication Proteins, Shere Lynne Myers

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This work investigates two questions regarding DNA replication. The first aim examines the interactions of DUE-B with replication proteins and the second explores the cellular effects of replicating a polypurine polypyrimidine sequence in human cells.

DUE-B siRNA decreases the chromatin binding of essential replication proteins Cdc45, PCNA and RPA. DUE-B also co-immunoprecipitates with Cdc45 and TopBP1. In vitro kinase assays suggest that the checkpoint protein ATR may phosphorylate DUE-B. These experiments lend further evidence that DUE-B plays an important role in the initiation of eukaryotic DNA replication.

To investigate the effects of replicating a polypurine-polypyrimidine sequence prone to secondary structure …

Novel Synthesis Of Polyhydrogenated Fullerenes, Angela Campo

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Hydrogenated fullerenes are of interest as a starting material in metal fulleride synthesis. By reacting C₆₀H₂ with various metals, alloyed metal fullerides could be produced. To achieve this goal, first a reliable synthesis of C₆₀H₂ must be determined. C₆₀H₂ is difficult to synthesis exclusively; C₆₀H₄ and C₆₀H₆ are also produced. Reduction of C₆₀ with NaBH₄ produced a mixture of products as well as excessive unreacted C₆₀. Attempts to modify this reaction to achieve C₆₀H₂ exclusively were unsuccessful. A …

A Screen Of One's Own The Tpec And Feminist Technological Textuality In The 21st Century, Amy J. Barnickel

Electronic Theses and Dissertations

In this dissertation, I analyze the 20th century text, A Room of One's Own, by Virginia Woolf (2005), and I engage with Woolf's concept of a woman's need for a room of her own in which she can be free to think for herself, study, write, or pursue other interests away from the oppression of patriarchal societal expectations and demands. Through library-based research, I identify four screens in Woolf's work through which she viewed and critiqued culture, and I use these screens to reconceptualize "a room of one's own" in 21st Century terms. I determine that the new "room" is …

The Rhetoric Of The Regional Image Interpreting The Visual Products Of Regional Plannning, Alissa Barber Torres

Electronic Theses and Dissertations

The Rhetoric of the Regional Image: Interpreting the Visual Products of Regional Planning investigates the manner in which visual conventions and visual contexts of regional visioning scenarios affect their interpretation by urban and regional planners, who use visual communication to meet the technical and rhetorical demands of their professional practice. The research assesses Central Florida‘s ―How Shall We Grow?‖ regional land use scenario using focus groups and interviews with planning professionals, a corresponding survey of community values, and rhetorical analysis to explore the ―How Shall We Grow?‖ scenario as persuasive communication. The Rhetoric of the Regional Image proposes specific recommendations …

Life Coaching: Possibilities For Occupational Therapy Practice, Amy L. Heinz, Karen Mccarthy, Wendy Pentland, Camille M. Dieterle, Jennie M. Antolak

Collected Faculty Scholarship

No abstract available