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The Significance Of Gender In Literacy Instructions, Julie Kragness

Theses and Dissertations

No abstract provided.

Numerical Methods For Fmri Data Analysis, Geethmala Sridaran

Dartmouth College Master’s Theses

Brain imaging data are increasingly analyzed via a range of machine-learning methods. In this thesis, we discuss three specific contributions to the field of neuroimaging analysis methods: 1. To apply a recently-developed technique for identifying and viewing similarity structure in neuroimaging data, in which candidate representational structures are ranked; 2. Provide side-by-side analyses of neuroimaging data by a typical non-hierarchical (SVM) versus hierarchical (Decision Tree) machine-learning classification methods; and 3. To develop a novel programming environment for PyMVPA, a current popular analysis toolbox, such that users will be able to type a small number of packaged commands to carry out …

Loss Of Gprc5a Enhances Survival In Normal And Malignant Lung Epithelial Cells By Eliciting Persistent Stat3 Activation Induced By Autocrine Lif, Yulong Chen

Dissertations and Theses (Open Access)

Signal transduction and activator of transcription 3 (Stat3) is activated by cytokines and growth factors in many cancers. Persistent activation of Stat3 plays important role in cell growth, survival, and transformation through regulating its targeted genes.

Previously, we found that mice with a deletion of the G protein-coupled receptor, family C, group 5, member a (Gprc5a) gene develop lung tumors indicating that Gprc5a is a tumor suppressor. In the present study, we examined he mechanism of Gprc5a-mediated tumor suppression. We found that epithelial cells from Gprc5a knockout mouse lung (Gprc5a^-/- cells) survive better in vitro in medium deprived …

Effect Of Acute Administration Of Angiopoietin-1 In Experimental Traumatic Spinal Cord Injury: Magnetic Resonance Imaging And Neurobehavioral Studies, Chirag B. Patel

Dissertations and Theses (Open Access)

Spinal cord injury (SCI) is a devastating condition that affects people in the prime of their lives. A myriad of vascular events occur after SCI, each of which contributes to the evolving pathology. The primary trauma causes mechanical damage to blood vessels, resulting in hemorrhage. The blood-spinal cord barrier (BSCB), a neurovascular unit that limits passage of most agents from systemic circulation to the central nervous system, breaks down, resulting in inflammation, scar formation, and other sequelae. Protracted BSCB disruption may exacerbate cellular injury and hinder neurobehavioral recovery in SCI.

In these studies, angiopoietin-1 (Ang1), an agent known to reduce …

Artemis Interacts With The Cul4a Ubiquitin E3 Ligase Complex And Regulates The Cell Cycle Progression, Yiyi Yan

Dissertations and Theses (Open Access)

Artemis, a member of the SNM1 gene family, is one of the six known components of the non-homologous end joining pathway. It is a multifunctional phospho-protein that has been shown to be modified by the phosphatidylinositol 3-kinases (PIKs) DNA-PKcs, ATM and ATR in response to a variety of cellular stresses. Artemis has important roles in V(D)J recombination, DNA double strand breaks repair and damage-induced cell-cycle checkpoint regulation. The detailed mechanism by which Artemis mediates its functions in these cellular pathways needs to be further elucidated. My work presented here demonstrates a new function for Artemis in cell cycle regulation as …

Characterization Of Alpha-Galactosylceramide As A Mucosal Adjuvant, Amy N. Courtney

Dissertations and Theses (Open Access)

Adjuvants are essential components of vaccine formulations that enhance adaptive immune responses to antigens, particularly for immunizations targeting the tolerogenic mucosal tissues, which are more biologically relevant for protective immunity against pathogens transmitted by the mucosal routes. Adjuvants possess the inherent capacity to bridge innate and adaptive immune responses through activating innate immune mediators. Here evidence is presented in support of the effectiveness of a synthetic glycolipid, alpha-Galactosylceramide (-GalCer), as an adjuvant for mucosal immunization with peptide and protein antigens, by oral and intranasal routes, to prime antigen-specific immune responses in multiple systemic and mucosal compartments. The adjuvant activity of …

An Innovative Phase I Trial Design Allowing For The Identification Of Multiple Potential Maximum To Lerated Doses With Combination Therapy Of Targeted Agents, Sarina A. Piha-Paul

Dissertations and Theses (Open Access)

Treatment for cancer often involves combination therapies used both in medical practice and clinical trials. Korn and Simon listed three reasons for the utility of combinations: 1) biochemical synergism, 2) differential susceptibility of tumor cells to different agents, and 3) higher achievable dose intensity by exploiting non-overlapping toxicities to the host. Even if the toxicity profile of each agent of a given combination is known, the toxicity profile of the agents used in combination must be established. Thus, caution is required when designing and evaluating trials with combination therapies. Traditional clinical design is based on the consideration of a single …

Targeting The Blood-Brain Barrier With A Non-Canonical Iron-Mimicry Mechanism, Michael G. Ozawa

Dissertations and Theses (Open Access)

Treatment of central nervous system (CNS) diseases is limited by the blood-brain barrier (BBB), a selective vascular interface restricting passage of most molecules from blood into brain. Specific transport systems have evolved allowing circulating polar molecules to cross the BBB and gain access to the brain parenchyma. However, to date, few ligands exploiting such systems have proven clinically viable in the setting of CNS diseases.

We reasoned that combinatorial phage-display screenings in vivo would yield peptides capable of crossing the BBB and allow for the development of ligand-directed targeting strategies of the brain. Here we show the identification of a …

E2f1 And Tumor Suppression: The Role Of P21, Mirnas, And The Dna Damage Response, Regina L. Weaks

Dissertations and Theses (Open Access)

E2F1 is a multi-faceted protein that has roles in a number of important cellular processes including cell cycle regulation, apoptosis, proliferation, and the DNA damage response (DDR). Moreover, E2F1 has opposing roles in tumor development, acting as either a tumor suppressor or an oncogene depending on the context. In human cancer, E2F1 is often deregulated through aberrations in the Rb-p16INK4a-cyclin D1 pathway. In these studies we examined three mechanisms by which E2F1 might mediate its tumor suppressive properties: p21-induced senescence, miRNAs, and the DNA damage response. We found that E2F1 acts as a tumor suppressor in response to ras activation …

Direct Inputs To Off Α And G9 Ganglion Cells From Aii Amacrine Cells In Rabbit Retina, Wei-Li Liu

Dissertations and Theses (Open Access)

In the mammalian retina, AII amacrine cells are essential in the rod pathway for dark-adapted vision. But they also have a “day job”, to provide inhibitory inputs to certain OFF ganglion cells in photopic conditions. This is known as crossover inhibition. Physiological evidence from several different labs implies that AII amacrine cells provide direct input to certain OFF ganglion cells. However, previous EM analysis of the rabbit retina suggests that the dominant output of the AII amacrine cell in sublamina a goes to OFF cone bipolar cells (Strettoi et al., 1992).

Two OFF ganglion cell types in the rabbit retina, …

A Metagenomic Study Of The Tick Midgut, Daniel T. Yuan

Dissertations and Theses (Open Access)

A Metagenomic Study of the Tick Midgut
Daniel Yuan, B.S.
Supervisory Professor : Steven J. Norris, Ph.D.
Southern tick–associated rash illness (STARI) or Master’s disease is a Lyme-like illness that occurs following bites by Amblyomma americanum, the lone-star tick. Clinical symptoms include a bull’s eye rash similar to the erythema migrans lesions of Lyme disease, as well as fever and joint pains. Lyme disease is caused by Borrelia burgdorferi and related spirochetes. However, B. burgdorferi has not been detected in STARI patients, or in ticks in the South Central U.S. The causative agent of STARI has not been identified, …

Modeling Sporadic Tumor Formation Driven By Telomere Dysfunction In The Gastrointestinal Tract, Suzanne S. Chan

Dissertations and Theses (Open Access)

Colorectal cancer is a complex disease that is thought to arise when cells accumulate mutations that allow for uncontrolled growth. There are several recognized mechanisms for generating such mutations in sporadic colon cancer; one of which is chromosomal instability (CIN). One hypothesized driver of CIN in cancer is the improper repair of dysfunctional telomeres. Telomeres comprise the linear ends of chromosomes and play a dual role in cancer. Its length is maintained by the ribonucleoprotein, telomerase, which is not a normally expressed in somatic cells and as cells divide, telomeres continuously shorten. Critically shortened telomeres are considered dysfunctional as they …

Specific, Reversible Cytostatic Protection Of Normal Cells Against Negative Effects Of Chemotherapy, Benjamin B. Mull

Dissertations and Theses (Open Access)

Chemotherapy is a common and effective method to treat many forms of cancer. However, treatment of cancer with chemotherapy has severe side effects which often limit the doses of therapy administered. Because some cancer chemotherapeutics target proliferating cells and tissues, all dividing cells, whether normal or tumor, are affected. Cell culture studies have demonstrated that UCN-01 is able to reversibly and selectively arrest normal dividing cells; tumor cells lines do not undergo this temporary arrest. Following UCN-01 treatment, normal cells displayed a 50-fold increase in IC50 for camptothecin; tumor cells showed no such increased tolerance.

We have examined the response …

The Effects Of Headlight Intensity And Clothing Contrast On Pedestrians' Own Estimated Recognition Distances At Night, Rachel Rosenberg

All Theses

Inadequate pedestrian detection is a crucial contributing factor in fatal nighttime collisions involving pedestrians. Pedestrians typically overestimate how recognizable they are to oncoming drivers and little is known about what affects pedestrians' estimates of how recognizable they think they are. This study explored the extent to which pedestrians believed their conspicuity was affected by headlight intensity and clothing reflectance. Participants in four clothing conditions and in four different levels of headlight intensity walked to and from a parked vehicle until they felt recognizable to the driver. Estimated recognition distances did not change with variations in headlight intensity, suggesting that pedestrians …

The Effect Of Predation And Immigration On Aquatic Macroinvertebrate Community Structure, Jenna Stanek

All Theses

To assess relative influences of local and regional processes, I created different intensities of predation (local process) and immigration (regional process) in an enclosure/exclosure field experiment on aquatic macroinvertebrate communities in a stream ecosystem. Twenty-four enclosures were manipulated in a two level, full factorial, repeated measures design which created four treatments: high predation/high immigration; high predation/low immigration; low predation/high immigration; and low predation/low immigration. I conducted Non-metric Multidimensional Scaling and Principal Coordinate Analysis on community data to determine differences in treatments. To look at changes in trophic structure, I quantified the percentage of each functional feeding group for each sampling …

Understanding The Role Of Electrostatics On Protein-Protein Binding, Rooplekha C Mitra

All Theses

Ionized groups carry net charge and thus play a major role in the electrostatic interactions between the ligand and receptor. So understanding the role of electrostatics on protein-protein interactions is crucial for understanding the contribution of ionizable groups to the binding. However, their ionization states depend on many factors including pH of water phase. The complexity comes from the fact that the pKa's of ionizable groups may be quite different from their standard values and even may change due to protein-protein binding. The main difficulty in modeling plausible ionization changes induced by the complex formation arises from the differences in …

Cultural Variation In The Visual Aspects Of Television Commercials: Comparing The Use And Effect Of Field Information In Chinese And Us Television Commercials, Jin Liu

All Theses

Cross-cultural psychology literature demonstrates cultural variation in visual perception patterns and field information inclusion in static visual designs between Chinese and American cultures. This study attempts to empirically investigate whether such variation exists in these two cultures' video images such as television commercials, and whether the commercials customized to cultural preference are more effective than others as previous research suggests. The research results correspond to previous claims about the visual design patterns in Chinese and American cultures, based on which suggestions for cinematic practice are provided. This research contends that variation in information inclusion and exclusion rules manifests differences in …

Hemocompatibility And Endothelialization Of Elastin And Collagen Scaffolds: The Role Of Plasma Proteins, Shear Stress And Integrins, Nithya Swaminathan

All Theses

Cardiovascular diseases are the leading cause of death worldwide as reported by the World Health Organization. Biomaterials have been extensively used in blood-contacting applications as heart valves, stents, grafts and catheters. However failure due to thrombosis hinders the long term patency and potential of these devices. Purified elastin scaffolds derived from porcine arteries as potential vascular graft materials are being investigated in our laboratory. In this study, we investigated the influence of factors such as shear stress and the presence of plasma proteins on the platelet response, and endothelial cell retention on purified elastin scaffolds to evaluate their hemocompatibility.

Elastin …

The Wilderness In Wilderness Therapy Techniques, Jane Brison

All Theses

The fields of Therapeutic Recreation, Criminal Justice, and Psychology currently use wilderness therapy techniques in the treatment of adolescents experiencing difficult life transitions. While literature exists that reports on the effectiveness of Wilderness Therapy techniques in addressing the issues that adolescents' face, there is a lack of understanding regarding exactly why these programs work. One theme that has been minimally addressed in the literature is the role that the 'wilderness' plays in these therapeutic techniques. People's perceptions of the wilderness environment vary due to experience and exposure. These varying perceptions could play a role in the effectiveness of these techniques …

Comparing The Effectiveness And Acceptability Of The Jump Into Foods And Fitness (Jiff) And Quest For Health Nutrition Interventions Within An African-American Population, Jermaine Shaw

All Theses

The severity of the obesity epidemic has increased the necessity for nutrition education programs. These programs must be culturally and age appropriate in order to be more effective in bringing about positive behavior changes in the target populations.
This study examined the effectiveness and acceptability of the Jump into Foods and Fitness (JIFF) and Quest for Health nutrition education programs within a pre-adolescent African-American population. Two classes from the Boys and Girls Club of Sumter, S.C. were randomly assigned to one of the nutrition education programs. Class one was assigned to the JIFF curriculum, and included 23 participants. Class two …

Sensor Based Nitrogen Management For Cotton Production In Coastal Plain Soils, Wesley Porter

All Theses

The main objective of this four year study was to develop, refine, and employ sensor-based algorithims to determine the mid season nitrogen requirements for production of irrigated and dryland cotton (Gossypium hirsutum L.) in Coastal Plains soils. The secondary objective of the project was to develop and test equipment for variable rate application of nitrogen to commerical cotton fields utilizing the developed algorithim. Two different production fields at Clemson's Edisto Research and Education Center near Blackville, SC were used. One field, equiped with an overhead irrigation system, was used during the 2007 and 2010 production seasons to develop the algorithm …

Genome-Wide Screening For Functional Factors In Listeria Monocytogenes Biofilm Formation, Yong Ouyang

All Theses

ABSTRACT
Listeria monocytogenes is a ubiquitous gram positive food borne pathogen. Ingestion of L.monocytogenes contaminated food can cause serious infections in immune-compromised persons. In addition to planktonic growth, this pathogen can also grow as biofilms under adverse conditions, which has been proved to be more resistant than its planktonic counterpart to various eradications, such as antibiotic treatments. Compared with the extensively studied intracellular replication mechanisms, L.monocytogenes biofilm developmental process is not well understood.
Our research group initiated a systemic study on the molecular mechanisms of L.monocytogenes biofilm formation. A whole genome-scale screening for functional factors involved in L.monocytogenes biofilm development …

In Vitro Analysis Of Auto-Loom Woven Test Patches For Hernia Repair, Bryant Mersereau

All Theses

Hernias are defects in the layers of the abdominal wall that can cause discomfort or pain and lead to serious health problems if left untreated. A significant portion of the world's population is afflicted by hernia formation, and the cost of treating those affected is in the billions of dollars in the US alone. The current best practices for repairing hernias involve the surgical implantation of polymeric meshes over and around the defect site. The mesh, acting as a synthetic replacement for the damaged abdominal wall layers, provides a barrier to further visceral protrusions through the defect, a support framework …

Autecology Of The Timber Rattlesnake (Crotalus Horridus) In The South Carolina Mountains., Jeff Mohr

All Dissertations

Although the most common rattlesnake in the eastern United States, the timber rattlesnake (Crotalus horridus) is little studied and remains poorly understood. Furthermore, populations have been severely diminished throughout its range and only a few scattered metapopulations remain in what was once a large and extensive North American range. Whereas some timber rattlesnake populations in woodland communities of the northeastern and western US have been studied, information on those occurring along the southern part of its range is virtually non-existent. In South Carolina there has been relatively little research done on this rattlesnake and there has been no formal study …

Are Distracted Drivers Aware That They Are Distracted?: Exploring Awareness, Self-Regulation, And Performance In Drivers Performing Secondary Tasks, Matthew Crisler

All Dissertations

Research suggests that driving while talking on a mobile telephone causes drivers not to respond to important events but has a smaller effect on their lane-keeping ability. This pattern is similar to research on night driving and suggests that problems associated with distraction may parallel those of night driving. Here, participants evaluated their driving performance before and after driving a simulated curvy road under different distraction conditions. In experiment 1 drivers failed to appreciate their distraction-induced performance decrements and did not recognize the dissociation between lane-keeping and identification. In Experiment 2 drivers did not adjust their speed to offset being …

Modeling Of Cell Transfer And Process-Induced Cell Damage In Laser-Assisted Cell Direct Writing, Wei Wang

All Dissertations

Laser-assisted cell direct-write technique has been obtaining more and more attention in different biomaterial direct writing applications. A typical laser-assisted cell direct-write process can be divided into two main stages: the cell droplet ejection and cell droplet landing. The objective of this study is to model the cell mechanical profile during the cell droplet ejection and cell landing and further model the cell damage.
The possible cell damage during the droplet ejection process in laser-assisted cell direct writing may come from two different sources: the phase explosion-induced bubble expansion and the thermoelastic stress wave. The bubble expansion-induced stress wave is …

The Role Of Car And Pxr In Toxicant Sensitivity, Linda Mota

All Dissertations

The Constitutive Androstane Receptor (CAR) and the Pregnane X Receptor (PXR) are nuclear receptors of significant importance in the regulation of enzymes that metabolize, detoxify and eliminate compounds from the body. In this study we assessed the protective role of CAR and PXR in the basal and inducible regulation of Cytrochrome P450s (CYPs), and the potential of CAR and PXR to help protect individuals from the organophosphate, parathion and the plasticizer, nonylphenol, putatively due to improved metabolism and elimination. Knockout models of these receptors were used to model susceptible populations such as children that are known to have lower CAR …

An Exogenous Approach To Circumventing Demolition By Neglect: The Impact Of Agricultural Preservation On Historic Colonial Towns, Galen Newman

All Dissertations

The effects of city decentralization and counter-urbanization of the American landscape have resulted in simultaneous negative impacts on both historic structures and agricultural landscapes. Rapid conversion of farmland has helped to facilitate the relocation of both populations and commercial activities in communities across the United States, leaving inner cities replete with functionless, unused, and unmaintained heritage structures. As civic core areas have become shells of their former selves, many once-vital structures have been removed while others have been abandoned and left to decay--a process known as demolition by neglect. While historic preservation efforts have attempted to salvage these historic structures, …

An Examination Of Heritage Resource Internship Experiences And Land Management Agency's Role In Inspiring Emerging Leaders, Carin Vadala

All Dissertations

To address the shortage of people seeking to fill employment vacancies, the land management agencies are using the Student Conservation Association (SCA) and internships as a means of recruiting the next generation of leaders. Yet little research has been conducted on internships in cultural and natural heritage resource parks. This study used a two-phase linear exploratory method to investigate the experiences of cultural and natural heritage resource interns. Additionally, the study sought to determine if and how internships shape vocational, recreational, social and personal identities concerning parks and protected areas. Using significant life experiences, environmental socialization and emerging adulthood theories, …

Chemical Modification And Biological Activity Exploration Of The Natural Product-Gossypol, Juanjuan Yin

All Dissertations

Gossypol is a naturally occurring, highly colored yellow pigment indigenous to the small intercellular pigment glands of the cotton plant genus Gossypium, which is proposed to be part of plant's defense system. Gossypol is characterized by wide contraceptive, antiviral, anticancer and antifungal properties. However, the relatively high toxicity of gossypol precludes its application in medical therapy. For this reason the syntheses and tests of gossypol derivatives, have been tried to enable their application as drugs. In our study, gossypol based methylation, glycosylation and nanoconjugate reactions were explored. The gossypol derivatives were all fully characterized by NMR, MS, FT-IR, UV spectrometry, …