Digital Commons Network^™

Targeted Deletion Of Hepatocyte Abca1 Leads To Very Low Density Lipoprotein Triglyceride Overproduction And Low Density Lipoprotein Hypercatabolism, Soonkyu Chung, Jenelle M. Timmins, Myngan Duong, Chiara Degirolamo, Shunxing Rong, Janet K. Sawyer, Roshni R. Singaraja, Michael R. Hayden, Nobuyo Maeda, Lawrence L. Rudel, Gregory S. Shelness, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Loss of ABCA1 activity in Tangier disease (TD) is associated with abnormal apoB lipoprotein (Lp) metabolism in addition to the complete absence of high density lipoprotein (HDL). We used hepatocyte-specific ABCA1 knock-out (HSKO) mice to test the hypothesis that hepatic ABCA1 plays dual roles in regulating Lp metabolism and nascent HDL formation. HSKO mice recapitulated the TD lipid phenotype with postprandial hypertriglyceridemia, markedly decreased LDL, and near absence of HDL. Triglyceride (TG) secretion was 2-fold higher in HSKO compared with wild type mice, primarily due to secretion of larger TG-enriched VLDL secondary to reduced hepatic phosphatidylinositol 3-kinase signaling. HSKO mice …

A Novel Role For Abca1-Generated Large Pre- Β Migrating Nascent Hdl In The Regulation Of Hepatic Vldl Triglyceride Secretion, Soonkyu Chung, Abraham K. Gebre, Jeongmin Seo, Gregory S. Shelness, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

In Tangier disease, absence of ATP binding cassette transporter A1 (ABCA1) results in reduced plasma HDL and elevated triglyceride (TG) levels. We hypothesized that hepatocyte ABCA1 regulates VLDL TG secretion through nascent HDL production. Silencing of ABCA1 expression in oleate-stimulated rat hepatoma cells resulted in: 1) decreased large nascent HDL (>10 nm diameter) and increased small nascent HDL (<10 nm) formation, 2) increased large buoyant VLDL1 particle secretion, and 3) decreased phosphatidylinositol-3 (PI3) kinase activation. Nascent HDL-containing conditioned medium from rat hepatoma cells or HEK293 cells transfected with ABCA1 was effective in increasing PI3 kinase activation and reducing VLDL TG secretion in ABCA1-silenced hepatoma cells. Addition of isolated large nascent HDL particles to ABCA1-silenced hepatoma cells inhibited VLDL TG secretion to a greater extent than small nascent HDL. Similarly, addition of recombinant HDL, but not human plasma HDL, was effective in attenuating TG secretion and increasing PI3 kinase activation in ABCA1-silenced cells. Collectively, these data suggest that large nascent HDL particles, assembled by hepatic ABCA1, generate a PI3 kinase-mediated autocrine signal that attenuates VLDL maturation and TG secretion. This pathway may explain the elevated plasma TG concentration that occurs in most Tangier subjects and may also account, in part, for the inverse relationship between plasma HDL and TG concentrations in individuals with compromised ABCA1 function.

Niemann-Pick C1-Like 1 Deletion In Mice Prevents High-Fat Diet-Induced Fatty Liver By Reducing Lipogenesis, Lin Jia, Yinyan Ma, Shunxing Rong, Jenna L. Betters, Ping Xie, Soonkyu Chung, Nanping Wang, Weiqing Tang, Liqing Yu

Department of Nutrition and Health Sciences: Faculty Publications

Niemann-Pick C1-Like 1 (NPC1L1) mediates intestinal absorption of dietary and biliary cholesterol. Ezetimibe, by inhibiting NPC1L1 function, is widely used to treat hypercholesterolemia in humans. Interestingly, ezetimibe treatment appears to attenuate hepatic steatosis in rodents and humans without a defined mechanism. Overconsumption of a high-fat diet (HFD) represents a major cause of metabolic disorders including fatty liver. To determine whether and how NPC1L1 deficiency prevents HFD-induced hepatic steatosis, in this study, we fed NPC1L1 knockout (L1-KO) mice and their wild-type (WT) controls an HFD, and found that 24 weeks of HFD feeding causes no fatty liver in L1-KO mice. Hepatic …

How Specialists Can Be Generalists: Resolving The "Parasite Paradox" And Implications For Emerging Infectious Disease, Salvatore J. Agosta, Niklas Janz, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The parasite paradox arises from the dual observations that parasites (broadly construed, including phytophagous insects) are resource specialists with restricted host ranges, and yet shifts onto relatively unrelated hosts are common in the phylogenetic diversification of parasite lineages and directly observable in ecological time. We synthesize the emerging solution to this paradox: phenotypic flexibility and phylogenetic conservatism in traits related to resource use, grouped under the term ecological fitting, provide substantial opportunities for rapid host switching in changing environments, in the absence of the evolution of novel host-utilization capabilities. We discuss mechanisms behind ecological fitting, its implications for defining specialists …

First Report Of Viral Pathogens Wssv And Ihhnv In Argentine Crustaceans [Note], Sergio Roberto Martorelli, Robin M. Overstreet, Jean A. Jovanovich Alvillar

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The pathogenic penaeid shrimp viruses white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV) are reported for the first time from Argentina. They both occurred in wild crustaceans in Bahia Blanca Estuary, with WSSV detected by polymerase chain reaction (pCR) or real time quantitative pCR (qpCR) methods as infecting samples as high as 56% of the penaeid Artemesia longinaris Bate, 1888, 67% of the grapsoid Cyrtograpsus angulatus dana, 1851, and 40% of the introduced palaemonid Palaemon macrodactylus Rathbun, 1902. The highest detected copy number was 39,600 copies/μg dNA. IHHNV was tested for using pCR in …

Barcoding Bushmeat: Molecular Identification Of Central African And South American Harvested Vertebrates, Mitchell J. Eaton, Greta J. Meyers, Sergios-Orestis Kolokotronis, Matthew S. Leslie, Andrew P. Martin, George Amato

United States Geological Survey: Staff Publications

The creation and use of a globally available database of DNA sequences from a standardized gene region has been proposed as a tool for species identification, assessing genetic diversity and monitoring the legal and illegal trade in wildlife species. Here, we contribute to the Barcode of Life Data System and test whether a short region of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene would reliably distinguish among a suite of commonly hunted African and South American mammal and reptile species. We used universal primers to generate reference barcode sequences of 645 bp for 23 species from five vertebrate …

Barcoding Bushmeat: Molecular Identification Of Central African And South American Harvested Vertebrates, Mitchell J. Eaton, Greta L. Meyers, Sergios-Orestis Kolokotronis, Matthew S. Leslie, Andrew P. Martin, George Amato

United States Geological Survey: Staff Publications

The creation and use of a globally available database of DNA sequences from a standardized gene region has been proposed as a tool for species identification, assessing genetic diversity and monitoring the legal and illegal trade in wildlife species. Here, we contribute to the Barcode of Life Data System and test whether a short region of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene would reliably distinguish among a suite of commonly hunted African and South American mammal and reptile species. We used universal primers to generate reference barcode sequences of 645 bp for 23 species from five vertebrate …

Security And Performance Analysis For Rfid Protocols, Bing Liang

Dissertations and Theses Collection (Open Access)

Radio Frequency Identification (RFID) is an advanced object identification technology that has already been applied in various industries. However, the insecure nature of the communication channel between readers and tags makes RFID systems vulnerable to various kinds of attacks. In recent years, many new methods have been proposed to improve the security of RFID systems, such as disabling tags, agent management and establishing cryptographic protocols. Among them, we focus on the last approach, which is more economic and convenient in certain cases. The first part of our work is to categorize typical existing RFID protocols according to their security levels. …

Does Dual Task Interference Affect Concurrent Duration Production?, Mary Merlau

Theses

Time perception has been cited as a good measure of workload because it seldom interferes with performance of tasks that do not require time estimation, is easy to implement, and is sensitive to task difficulty (Hart, 1975a; Zakay & Shub, 1998). It was hypothesized that duration productions could be used to measure workload caused by two word tasks that would interfere with one another as outlined by Wickens' (1980) multiple resource theory. To test this hypothesis participants estimated a 15 second duration while performing a visual animal name detection task, along with an auditory animal name detection task, or a …

Admissions Viewbook, 2010-2011, Illinois State University, Office Of Admissions

Admissions Viewbook

Recruitment booklet created by Illinois State University's Office of Admissions

From The Classroom To The Courtroom: Intelligent Design And The Constitution, Jay D. Wexler

Faculty Scholarship

Although the Supreme Court of the United States has never developed a single clear test for determining what kinds of state action violate the Establishment Clause of the First Amendment, schools that attempt to teach or introduce intelligent design as a purportedly scientific alternative to evolution likely fall afoul of the First Amendment's commands. Under the Court's most relevant precedent, Edwards v. Aguillard, teaching intelligent design violates the Establishment Clause because, among other things, there is an enormous disconnect between the purpose of teaching intelligent design and its effect. Moreover, public school teachers do not possess any First Amendment right …

A Study Of Recent Classification Algorithms And A Novel Approach For Biosignal Data Classification, Eyup Cinar

Theses

Analyzing and understanding human biosignals have been important research areas that have many practical applications in everyday life. For example, Brain Computer Interface is a research area that studies the connection between the human brain and external systems by processing and learning the brain signals called Electroencephalography (EEG) signals. Similarly, various assistive robotics applications are being developed to interpret eye or muscle signals in humans in order to provide control inputs for external devices. The efficiency for all of these applications depends heavily on being able to process and classify human biosignals. Therefore many techniques from Signal Processing and Machine …

Infrastructure Investment Strategies For Increased Agro-Industrial Outputs In Kosovo : [Presentation Given August 12, 2010], Siti Insiana

Theses

Not provided.

The Development Of A Model Program For The Effective Management Of Biomedical Waste In The Caribbean. A Review Of Barriers Within Public Hospitals In The Caribbean., Andrea Spence

Theses

When biomedical waste is improperly managed it poses a health hazard and risk to healthcare workers, sanitation workers and the general public in contracting infectious/dangerous diseases. Biomedical waste is also generated from individuals in their homes from the use of syringes or diagnostic lancets that are not properly managed. Biomedical waste is considered special waste and has a health and environmental hazard that can result in infections and diseases such as HIV/AIDs. Discarded sharps pose a health risk to the public particularly solid waste workers who may suffer a needle stick injury if they are not properly packaged for disposal. …

Are National Class Actions Constitutional?: A Reply To Hogg And Mckee, Janet Walker

Osgoode Hall Law Journal

This article argues that there is no constitutional impediment to the certification of multijurisdictional class actions by provincial superior courts, and no constitutional requirement to confine plaintiff classes to those in which each claim has a real and substantial connection to the forum. Neither the text of the Constitution nor the constitutionally mandated rules of the conflict of laws restrict court jurisdiction in this way. Rather, the principles of order and fairness require Canadian courts to exercise jurisdiction over multi-jurisdictional class actions in a way that maximizes the objectives of class actions, and minimizes the incidence of overlapping classes and …

Hate Speech And The Language Of Racism In Latin America: A Lens For Reconsidering Global Hate Speech Restrictions And Legislation Models, Tanya K. Hernandez

Faculty Scholarship

In Latin America, like many countries in Europe, hate speech is prohibited. Yet Latin America is rarely included in the transnational discussion regarding the regulation of hate speech. Instead, the discourse focuses on a comparison of the advisability of Europe's hate speech regulations and free speech acceptance of hate speech in the United States. As a result, the ability to fundamentally examine the connections between hate speech and inequality, in addition to the most effective legal mechanisms for addressing it, is undermined. It is especially critical to broaden the hate speech debate now that we are seeing an apparent rise …

Value Of Intersectional Comparative Analysis To The Post-Racial Future Of Critical Race Theory: A Brazil-U.S. Comparative Case Study, The Commentary: Critical Race Theory: A Commemoration: Response, Tanya K. Hernandez

Faculty Scholarship

This Commentary Article aims to illustrate the value of comparative law to the jurisprudence of Critical Race Theory (CRT), particularly with reference to the CRT project of deconstructing the mystique of "postracialism. " The central thesis of the Article is that the dangerous seductions of a U.S. ideology of "post-racialism" are more clearly identified when subject to the comparative law lens. In particular, a comparison to the Brazilian racial democracy version of "post-racialism"is an instructive platform from which to assess the advisability of promoting post-racial analyses of U.S. racial inequality. In Part I the Article introduces the value of comparative …

The Consequence Of Human Differences, Jospeh Vining

Articles

This essay explores the ways in which the recognition of individual and person in the legal form of thought distinguishes it from forms of thought in evolutionary biology and mathematics that are put forward as means to a complete picture of the world. The essay observes that the legal form of thought is in fact deeply involved in our modern understanding of Nature itself.

A Framework For Active Learning: Revisited, Yue Chen

Master's Projects

Over the past decade, algorithm visualization tools have been researched and developed to be used by Computer Science instructors to ease students’ learning curve for new concepts. However, limitations such as rigid animation frameworks, lack of user interaction with the visualization created, and learning a new language and environment, have severely reduced instructors’ desire to use such a tool. The purpose of this project is to create a tool that overcomes these limitations. Instructors do not have to get familiar with a new framework and learn another language. The API used to create algorithm animation for this project is through …

Parallel Programming Recipes, Thuy C. Nguyenphuc

Master's Projects

Parallel programming has become vital for the success of commercial applications since Moore’s Law will now be used to double the processors (or cores) per chip every technology generation. The performance of applications depends on how software executions can be mapped on the multi-core chip, and how efficiently they run the cores. Currently, the increase of parallelism in software development is necessary, not only for taking advantage of multi-core capability, but also for adapting and surviving in the new silicon implementation. This project will provide the performance characteristics of parallelism for some common algorithms or computations using different parallel languages. …

Geometrical Structure And Analysis Of Association Rule, Rajat Sar

Master's Projects

Association rule mining helps us to identify the association between items from a large transactional data set. It has always been a time consuming process because of repeatedly scanning of the data set. Apriori Algorithm [1] and FP-Tree Algorithm [2] are the two methods to find out the association of items in a large transactional item set. Both the above algorithm works differently (Apriori follows Bottom-Up Approach & FP-Tree follows Top-Down Approach) in order to get the association. Associations of items generated from the above two algorithms can be represented in geometry. The geometrical form of associations is called Simplical …

What The Body Stories Of Girls Tell Us About Autonomy And Connection During Adolescence, Colleen Mcmillan

Theses and Dissertations (Comprehensive)

This qualitative study aimed to understand what the body stories of girls who exhibit signs of disordered eating reveal about the concepts of autonomy and connection during early adolescence. The study was guided by the research question “Are the symptoms of disordered eating one of the ways the female body “talks” about the experience of disconnection during adolescence?” Informed by Relational Cultural Theory, data was collected from two focus groups of 16 adolescent girls aged 11 to 14 years. Each group met six times over a four month period. Because many aspects of lived experience cannot be expressed verbally, the …

Spatial And Spatial-Temporal Analysis Of Grizzly Bear Movement Patterns As Related To Underlying Landscapes Across Multiple Scales, Barbara Lynn Carra

Theses and Dissertations (Comprehensive)

Studying the movements of grizzly bears (Ursus arctos) in Alberta is imperative for scientifically informed management practices. To properly balance industry requirements with conservation imperatives, it is necessary to understand the spatial and spatial-temporal movement patterns of grizzly bears as they relate to underlying landscape properties. As part of the Foothills Research Institute Grizzly Bear Research Program, this dissertation explored both fine and largescale movement patterns generated from global positioning system (GPS) radiotelemetry data.

Between 1999 and 2005, grizzly bears were captured and radio-collared across western Alberta. The temporal resolution of GPS data collection had a large impact …

Tundra Snow Cover Properties From In-Situ Observation And Multi-Scale Passive Microwave Remote Sensing, Andrew Rees

Theses and Dissertations (Comprehensive)

Tundra snow cover is important to monitor as it influences local, regional, and global scale surface water balance, energy fluxes, and ecosystem and permafrost dynamics. Moreover, recent global circulation models (GCM) predict a pronounced shift in high latitude winter precipitation and mean annual air temperature due to the feedback between air temperature and snow extent. At regional and hemispheric scales, the estimation of snow extent, snow depth and, snow water equivalent (SWE) is important because high latitude snow cover both forces and reacts to atmospheric circulation patterns. Moreover, snow cover has implications on soil moisture dynamics, the depth, formation and …

Vocabulary And Phonological Awareness In 3- To 4-Year-Old Children: Effects Of A Training Program, Iuliana Elena Baciu

Theses and Dissertations (Comprehensive)

The impact of a preschool training program that combined a vocabulary instruction strategy with phonological awareness activities and instruction in the alphabetic principle, as well as incidental teaching of basic vocabulary items was evaluated using a pretest-posttest design with a control group. This language and literacy (LL) training targeted three to four year-old English as a second language (L2) learners and monolingual (L1) English speakers (n = 63) and was conducted twice a week for two hours, for a total of 24 weeks. Both language groups contained a low socioeconomic status (SES) and a middle SES groups. The children …

Dhamma Education: The Transmission And Reconfiguration Of The Sri Lankan Buddhist Tradition In Toronto, Deba Mitra Bhikkhu

Theses and Dissertations (Comprehensive)

This research documents the transition of a religious tradition from a foreign-born generation to a new generation who call Canada home. It examines how first-generation Sri Lankan Buddhists in Toronto transmit their Buddhist tradition to their Canadian children, how the latter receive what is passed on to them, and what happens within the tradition through the process of transmission and reception. These issues are of paramount concern not only for Buddhist communities in North America but also for other immigrant and refugee groups undergoing the process of resettlement. Working with two Sri Lankan temples in Toronto, the Toronto Mahavihara and …

Our Personal Core Identity: A Wesleyan Perspective, Jim Boetcher

The Asbury Journal

Humans have many relational identities that vary with changes in relationships such as being a mother or a daughter, but one's core identity remains independent of these social roles. This paper explores human personal core identity theologically by drawing on Methodism as it existed under the leadership of John Wesley, and scientifically by using three cutting edge scientific research projects: The Human Family Tree and The Human Genome Project present empirical evidence that all humans are genetically related and only one race exists - the human race. Discovering Ardi: Changing Our Understanding of Human Origins shows that humans did not …

Book Reviews, Michael Matlock, Bradley Johnson, Charles Meeks, Amos Yong, Joseph Okello, Kandace Brooks, J. Ellsworth Kalas

The Asbury Journal

BibleWorks. DVD-ROM and CD-ROM, version 8 2009. BibleWorks, LLC. $349
Reviewed by Michael D. Matlock and Bradly T. Johnson

Paul A. Hartog, ed.
The Contemporary Church and the Early Church: Case Studies in Ressourcement
Eugene, OR: Wipf and Stock, 2010
Reviewed by Charles Meeks

Gary B. McGee
Miracles, Missions, and American Pentecostalism
American Society of Missiology series 45
2010. Maryknoll: Orbis Books
Reviewed by Amos Yong

John R. Levison
Filled with the Spirit
Grand Rapids, Michigan: William B. Eerdmans Publishing, 2009.
Reviewed by Joseph B. O. Okello

Constance M. Cherry
The Worship Architect: A Blueprint for Designing Culturally Relevant and …

Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay, D. T. Gauthier, K. S. Reece, J. Xiao, M. W. Rhodes, H. I. Kator, R. J. Latour, C. F. Bonzek, J. M. Hoenig, W. K. Vogelbein

Biological Sciences Faculty Publications

Striped bass (Morone saxatilis) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli). Mycobacterium …

Morphological Differences Among Eyeless Amphipods In The Genus Stygobromus Dwelling In Different Subterranean Habitats, David C. Culver, John R. Holsinger, Mary C. Christman, Tanja Pipan

Biological Sciences Faculty Publications

The amphipod genus Stygobromus occurs in a variety of subterranean habitats in North America, including caves, phreatic (groundwater) lakes, and superficial subterranean habitats (seeps and epikarst). The habitats share the absence of light but differ in other features, such as pore size of the habitat, available food, and degree of seasonality. Measurements of body size, antennal size, and antennal segment number of type specimens were compared for 56 species occurring in the eastern United States. Except for differences in body size, differences among species in the four different habitats were not significant. Body size was related to relative pore size …