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Smooth Muscle-Specific Expression Of Neurotrophin-3 In The Embryonic And Neonatal Gastrointestinal Tract Of The Mouse, Jennifer Mcadams, Edward A. Fox

Department of Psychological Sciences Faculty Publications

Neurotrophin-3 (NT-3), which is essential for the survival of a large proportion of vagal sensory neurons, is expressed in the developing gastrointestinal (GI) tract where it could contribute to this survival, to other aspects of vagal afferent development, and to the development of spinal afferents, postganglionic sympathetic neurons and intrinsic neurons. Identifying the functions of this peripheral NT-3 requires a detailed understanding of the localization and timing of its expression in the developing GI tract. Therefore, X-gal staining of embryos and neonates expressing the lacZ reporter gene from the NT-3 locus was used to characterize the spatiotemporal pattern of NT-3 …

Effects Of The Novel Endocannabinoid Uptake Inhibitor, Ly2183240, On Fear-Potentiated Startle And Alcohol-Seeking Behaviors In Mice Selectively Bred For High Alcohol Preference, Matthew S. Powers, G. D. Barrenha, N. S. Mlinac, Eric L. Barker, Julia Chester

Department of Psychological Sciences Faculty Publications

Rationale Alcohol-use disorders often occur together with anxiety disorders in humans which may be partly due to common inherited genetic factors. Evidence suggests that the endocannabinoid system (ECS) is a promising therapeutic target for the treatment of individuals with anxiety and/or alcohol-use disorders. Objectives The present study assessed the effects of a novel endocannabinoid uptake inhibitor, LY2183240, on anxiety- and alcohol-seeking behaviors in a unique animal model that may represent increased genetic risk to develop co-morbid anxiety and alcohol-use disorders in humans. Mice selectively bred for high alcohol preference (HAP) show greater fear-potentiated startle (FPS) than mice selectively bred for …

Air-Side Heat Transfer Enhancement By A V-Formation Delta-Winglet Array In A Developing Channel Flow, Jing He, Anthony M. Jacobi

International Refrigeration and Air Conditioning Conference

No abstract provided.

Pentylenetetrazol Produces A State-Dependent Conditioned Place Aversion To Alcohol Withdrawal In Mice, Julia Chester, Laran E. Coon

Department of Psychological Sciences Faculty Publications

The purpose of this study was to determine if aversive effects of alcohol withdrawal could be detected in mice using the place conditioning procedure and whether the GABAA receptor antagonist, pentylenetetrazol (PTZ), would increase the aversive effects of alcohol withdrawal and increase the probability of detecting conditioned place aversion. Subjects were alcohol-naïve mice from a specific line selectively bred for low alcohol preference (LAP1; n=91) and were assigned to three groups: alcohol withdrawal, PTZ alone, and PTZ + alcohol withdrawal. On four trials, mice received either a 4.0 g/kg intraperitoneal (i.p.) injection of alcohol (alcohol withdrawal, PTZ + alcohol withdrawal …

What Is Mine, Elizabeth Kaiser

Electronic Theses and Dissertations

No abstract provided.

Modeling Mitochondrial Bioenergetics With Integrated Volume Dynamics., Jason N. Bazil, Gregery T. Buzzard, Ann E. Rundell

Department of Mathematics Faculty Publications

Mathematical models of mitochondrial bioenergetics provide powerful analytical tools to help interpret experimental data and facilitate experimental design for elucidating the supporting biochemical and physical processes. As a next step towards constructing a complete physiologically faithful mitochondrial bioenergetics model, a mathematical model was developed targeting the cardiac mitochondrial bioenergetic based upon previous efforts, and corroborated using both transient and steady state data. The model consists of several modified rate functions of mitochondrial bioenergetics, integrated calcium dynamics and a detailed description of the K+-cycle and its effect on mitochondrial bioenergetics and matrix volume regulation. Model simulations were used to fit 42 …

Large Scale Application Of Vibration Sensors For Fan Monitoring At Commercial Layer Hen Houses, Yan Chen, Ji-Qin Ni, Claude A. Diehl, Albert J. Heber, Bill W. Bogan, Li-Long Chai

School of Agricultural & Biological Engineering Faculty Publications

Continuously monitoring the operation of each individual fan can significantly improve the measurement quality of aerial pollutant emissions from animal buildings that have a large number of fans. To monitor the fan operation by detecting the fan vibration is a relatively new technique. A low-cost electronic vibration sensor was developed and commercialized. However, its large scale application has not yet been evaluated. This paper presents long-term performance results of this vibration sensor at two large commercial layer houses. Vibration sensors were installed on 164 fans of 130 cm diameter to continuously monitor the fan on/off status for two years. The …

Mice Deficient In Brain-Derived Neurotrophic Factor Have Altered Development Of Gastric Vagal Sensory Innervation, Michelle C. Murphy, Edward A. Fox

Department of Psychological Sciences Faculty Publications

Vagal sensory neurons are dependent on neurotrophins to survive programmed cell death during development. Here, the contribution of brain-derived neurotrophic factor (BDNF) to the survival of gastric vagal sensory afferents was investigated. Also, based on BDNF roles in other sensory systems, its effects on axon guidance and mechanoreceptor differentiation were examined. Postmortem anterograde tracing with 1, 1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) was used to selectively label vagal projections to the stomach on postnatal day (P)0, 3, 4, and 6 in wild types and heterozygous or homozygous BDNF mutants. Sampling sites distributed throughout the ventral stomach wall were scanned with a confocal microscope …

From Baby Formula To Solid Food: How The Influence Of Media Has Nourished Children's Literature, Nicole L. Wilson

Wayne State University Dissertations

This dissertation juxtaposes children's literature and media and investigates the resulting influence of media on literature. Jay Bolter and Richard Grusin's ideas regarding Remediation in conjunction with Henry Jenkins ideas regarding Convergence Culture provide the framework for my ideas regarding media and their roles in children's literature.

One way media influence children's literature is through the realm of nostalgia. Adults reflect on their childhoods and the literature they encountered. Their desires to recreate the truths they recall learning from literature lead them to produce reflective and restorative remediations of texts that allow modern youths new opportunities with canonical texts.

Another …

Analysing The Effects Of Loss Of Sin3 In Drosophila Melanogaster, Aishwarya Swaminathan

Wayne State University Dissertations

Sin3A has been previously shown to be an essential gene for Drosophila viability and is implicated in the regulation of cell cycle. In this study, we show that SIN3 is not only required for embryonic viability but also for post-embryonic development. Genetic analysis suggests that the different isoforms of SIN3 may regulate unique sets of genes during development. The developmental lethality occurring due to ubiquitous knock down of SIN3 is hypothesized to be to the result of defects in cell proliferation. Conditional knock down of SIN3 in the wing discs results in a curly wing phenotype in the adult fly. …

The Social Interactions Of Students With Disabilties In A 5th Grade Level Inclusive Classroom And The Effect On Academic Achievement, Estella Marshall-Reed

Wayne State University Dissertations

No abstract provided.

Drosophila Nicotinamidase And Sir2 In Longevity Regulation And Oxidative Stress Response, And The Implications For Aging Theory, Gregory Miller

Wayne State University Dissertations

The protein deacetylase Sir2 has been shown to increase lifespan in a variety of organisms, possibly through its ability to engender the effects of dietary restriction. The Drosophila nicotinamidase DNAAM increases lifespan in a Sir2 dependant manner, and may play a role in oxidative stress resistance. Oxidative damage that accumulates in cells during normal aging contributes to a variety of diseases and pathologies, and can result in an increase in age specific mortality. Accordingly, the oxidative stress theory of aging predicts that organisms with an increased tolerance to oxidative damage will have an increased longevity, while organisms with decreased tolerance …

Minor Courts And Communities At The Frontier: The Justice Of The Peace In Early Missouri, Bonnie Aileen Speck

Wayne State University Dissertations

ABSTRACT

MINOR COURTS AND COMMUNITIES AT THE FRONTIER JUSTICES OF THE PEACE IN EARLY MISSOURI

by

BONNIE A. SPECK

May 2011

Advisor: Sandra VanBurkleo

Major: American Legal and Constitutional History

Degree: History

This study focused on local and county courts operated by Missouri's justices of the peace between the Louisiana Purchase and roughly 1875. Its purpose was to investigate the role of township justices’ courts and county courts of commissioners in terms of interactions with local residents; effects of rulings and other court actions on everyday affairs; and wider impacts on Missouri society. Sources included territorial and …

Wastewater Treatment Comes To Detroit: Law, Politics, Technology And Funding, Barry Neal Johnson

Wayne State University Dissertations

Detroit was one of the cities identified by the International Joint Commission as polluting the Great Lakes in contravention of the Boundary Waters Treaty of 1909. The United States Public Health Service also reported on the pollution that entered the Detroit River from Detroit's sewers. Pollution by sewage threatened lives with a dramatic increase in cases of Typhoid Fever and other gastro intestinal sicknesses. Chlorination of water supplies reduced these incidents to an acceptable level minimizing the arguments of sanitarians proposing wastewater treatment. Expansion of the city to encompass a rapidly rising population channeled the city's financial resources into infrastructure. …

An Africentric Reading Protocol: The Speculative Fiction Of Octavia Butler And Tananarive Due, Tonja Lawrence

Wayne State University Dissertations

This examination of Africentric speculative fiction (ASF) applies an Africentric reading protocol to selected works of Octavia E. Butler and Tananarive Due. Butler's Parable Series and Due's African Immortals Series are examined using seven elements of Africentric narrative specific to cultural speculative fiction. Finally, I discuss the implications of using an Africentric reading protocol as an example of cultural analysis that can be adapted to the textual analysis of culturally specific works of fiction.

Post-Transcriptional Regulation Of Vibrio Cholerae Virulence Activator Toxt, Basel Hanna Abuaita

Wayne State University Dissertations

Vibrio cholera, the causative agent of the severe diarreal illness cholera, uses a complex array of gene regulation to induce its virulence determinants. During the early stage of infection, and upon response to unknown signals, virulence genes are turned on. ToxT protein is the primary virulence gene transcription activator. Once ToxT is produced, it amplifies its own expression through an auto-regulatory loop and directly binds and activates expression of various virulence factors including the toxin-coregulated pilus (TCP) and cholera toxin (CT). During the late stage of infection, virulence genes are turned off and the bacteria escape the host to resume …

Tracking Profiles Of Genomic Instability In Spontaneous Transformation And Tumorigenesis, Lesley Lawrenson

Wayne State University Dissertations

The dominant paradigm for cancer research focuses on the identification of specific genes for cancer causation and for the discovery of therapeutic targets. Alternatively, the current data emphasize the significance of karyotype heterogeneity in cancer progression over specific gene-based causes of cancer. Variability of a magnitude significant to shift cell populations from homogeneous diploid cells to a mosaic of structural and numerical chromosome alterations reflects the characteristic low-fidelity genome transfer of cancer cell populations. This transition marks the departure from micro-evolutionary gene-level change to macro-evolutionary change that facilitates the generation of many unique karyotypes within a cell population. Considering cancer …

The Interictal State In Epilepsy And Behavior, Daniel Tice Barkmeier

Wayne State University Dissertations

Epilepsy is one of the most common neurological diseases, affecting up to 1% of the world population. Epilepsy remains poorly understood and there are currently no medications to cure it. Patients with epilepsy have both seizures as well as another type of abnormal activity between seizures, known as interictal spikes. Interictal spikes have thus far been poorly researched, yet growing evidence supports an important role for them in epilepsy. In this project, we first show the high variability between reviewers in marking interictal spikes on intracranial EEG, and then develop and test an automated detection method to solve this problem. …

Activation Of Tumor Cell Death Program By Targeting The Ubiquitin-Proteasome Pathway: Significance In Cancer Treatment And Prevention, Michael Joseph Frezza

Wayne State University Dissertations

ACTIVATION OF TUMOR CELL DEATH PROGRAM BY TARGETING THE UBIQUITIN-PROTEASOME PATHWAY: SIGNIFICANCE IN CANCER TREATMENT AND PREVENTION

by

MICHAEL FREZZA

August 2010

Advisor: Dr. Q. Ping Dou

Major: Cancer Biology

Degree: Doctor of Philosophy

The ubiquitin-proteasome pathway serves as a quality control mechanism to regulate the degradation of intracellular proteins involved in a wide array of cellular processes including tumorigeneis. Thus targeting key features of protein turnover responsible for the growth and proliferation of cancer have emerged as a favorable approach in cancer therapy. Both in vitro and in vivo experimental and clinical results have demonstrated the potential use of …

Immunomodulatory Effect Of Host And Fungal Eicosanoids During Host-Pathogen Interactions With Candida Albicans, Gitanjali Kundu

Wayne State University Dissertations

Candida albicans, an opportunistic fungal pathogen, poses a significant clinical threat to immunocompromised patients. Diseases associated with this fungus ranges from superficial mucosal infection to life-threatening systemic candidiasis. The mechanisms by which Candida persists at mucosal surfaces in the face of an adaptive response are unclear. Candida produces immunomodulatory oxylipins that cross-react functionally with host eicosanoids, which are considered to play important role in regulating innate and adaptive immune responses. Our objective was to characterize the role of prostaglandins produced by the host and this fungus during host pathogen interactions, both in vitro with dendritic cells (DCs) and macrophages, and …

Determination Of The Essential Functions Of A Conserved Cyclin, Cyclin Y, In Drosophila, Dongmei Liu

Wayne State University Dissertations

The Drosophila gene CG14939 encodes a member of a highly conserved family of cyclins, the Y type cyclins, which have not been functionally characterized in any organism. Here I report the generation and phenotypic characterization of a null mutant of CG14939, which we rename Cyclin Y (CycY). I show that the null mutant, CycY^E8, is homozygous lethal with most mutant animals arresting during pupal development. The mutant exhibits delayed larval growth and major developmental defects during metamorphosis. Heat shock-induced expression of CycY at different times during development resulted in variable levels of rescue, the timing …

Evolution Of Lactate Dehydrogenase Genes In Primates, With Special Consideration Of Nucleotide Organization In Mammalian Promoters, Zack Papper

Wayne State University Dissertations

Concomitant with an increase in brain volume and mass, the allocation of energetic resources to the brain increased during stem anthropoid evolution, leading to humans. One mechanism by which this allocation may have occurred is through greater use of lactate as a neuronal fuel. Both the production of lactate, and conversion to pyruvate for use in aerobic metabolism, are catalyzed, in part, by the tetrameric enzyme lactate dehydrogenase (LDH). The two primary LDH genes, LDHA and LDHB, confer different rates of substrate turnover to the LDH enzyme, and these rates lend to the argument that LDHA supports anaerobic while LDHB …

Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target, Hanumantharao Paritala

Wayne State University Dissertations

N5-Carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase), a key enzyme in microbial de novo purine biosynthesis, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to N5-CAIR. To date, this enzyme has been observed only in microorganisms, and thus, it represents an ideal target for antimicrobial drug development. Here we report structural and functional studies on the Aspergillus clavatus N5-CAIR synthetase and identification of inhibitors for the enzyme. In collaboration with Dr. Hazel Holden of the University of Wisconsin, the three-dimensional structure of Aspergillus clavatus N5-CAIR synthetase was solved in the presence of either Mg2ATP or MgADP and AIR. These structures, determined to 2.1 …

Recommendations Regarding Establishment Of A Mediation Clinic, Cynthia A. Savage

Cardozo Journal of Conflict Resolution

This article proposes utilizing consistent terminology and goals and recommends methods and approaches for effective design and implementation of supervised, hands-on, mediation training programs within conflict resolution graduate programs. Although law school programs are not addressed directly in this article and might differ in some respects as to their goals, effective methods and approaches will be much the same regardless of the setting.

Part I of this article will discuss terminology and propose a consistent clinic definition and clinic goals, and Part II will explore and make recommendations concerning clinic design. Part III will outline additional recommendations. Part IV provides …

The High Cost Of Mandatory Arbitration, Martha Nimmer

Cardozo Journal of Conflict Resolution

This Note traces the development of voluntary and mandatory arbitration in the United States, including the reasons for the rise in arbitration and the implications for Americans' civil rights. In voluntary arbitration, an employee agrees to arbitrate a workplace dispute instead of litigating a claim. Mandatory arbitration, in contrast, "requires an employee, as a condition of employment, to forego all access to a jury trial and use arbitration in place of a judicial forum for resolving statutory and contractual claims." The use of mandatory arbitration, particularly in employment disputes arising under Title VII, has increased dramatically since 1991 as a …

Climate Control Of Terrestrial Carbon Exchange Across Biomes And Continents, Chuixiang Yi, Daniel Ricciuto, Runze Li, John Wolbeck, Xiyan Xu, Mats Nilsson, Luis Aires, John D. Albertson, Christof Ammann, M Altaf Arain, Alessandro C. De Araujo, Marc Aubinet, Mika Aurela, Zoltan Barcza, Alan Barr, Paul Berbigier, Jason Beringer, Christian Bernhofer, Andrew T. Black, Paul V. Bolstad, Fred C. Bosveld, Mark S J Broadmeadow, Nina Buchmann, Sean P. Burns, Pierre Cellier, Jingming Chen, Jiquan Chen, Philippe Ciais, Robert Clement, Bruce D. Cook, Peter S. Curtis, D Bryan Dail, Ebba Dellwik, Nicolas Delpierre, Ankur R. Desai, Sabina Dore, Danilo Dragoni, Bert G. Drake, Eric Dufrêne, Allison Dunn, Jan Elbers, Werner Eugster, Matthias Falk, Christian Feigenwinter, Lawrence B. Flanagan, Thomas Foken, John Frank, Juerg Fuhrer, Damiano Gianelle, Allen Goldstein, Mike Goulden, Andre Granier, Thomas Grünwald, Lianhong Gu, Haiqiang Guo, Albin Hammerle, Shijie Han, Niall P. Hanan, László Haszpra, Bernard Heinesch, Carole Helfter, Dimmie Hendriks, Lindsay B. Hutley, Andreas Ibrom, Cor Jacobs, Torbjörn Johansson, Marjan Jongen, Gabriel Katul, Gerard Kiely, Katja Klumpp, Alexander Knohl, Thomas Kolb, Werner L. Kutsch, Peter Lafleur, Tuomas Laurila, Ray Leuning, Anders Lindroth, Heping Liu, Benjamin Loubet, Giovanni Manca, Michal Marek, Hank A. Margolis, Timothy A. Martin, William J. Massman, Roser Matamala, Giorgio Matteucci, Harry Mccaughey, Lutz Merbold, Tilden Meyers, Mirco Migliavacca, Franco Miglietta, Laurent Misson, Meelis Mölder, John Moncrieff, Russell K. Monson, Leonardo Montagnani, Mario Montes-Helu, Eddy Moors, Christine Moureaux, Mukufute M. Mukelabai, J William Munger, May Myklebust, Zoltán Nagy, Asko Noormets, Walter Oechel, Ram Oren, Stephen G. Pallardy, Kyaw Tha Paw U, João S. Pereira, Kim Pilegaard, Krisztina Pintér, Casimiro Pio, Gabriel Pita, Thomas L. Powell, Serge Rambal, James T. Randerson, Celso Von Randow, Corinna Rebmann, Janne Rinne, Federica Rossi, Nigel Roulet, Ronald J. Ryel, Jorgen Sagerfors, Nobuko Saigusa, María José Sanz, Giuseppe-Scarascia Mugnozza, Hans Peter Schmid, Guenther Seufert, Mario Siqueira, Jean-François Soussana, Gregory Starr, Mark A. Sutton, John Tenhunen, Zoltán Tuba, Juha-Pekka Tuovinen, Riccardo Valentini, Christoph S. Vogel, Jingxin Wang, Shaoqiang Wang, Weiguo Wang, Lisa R. Welp, Xuefa Wen, Sonia Wharton, Matthew Wilkinson, Christopher A. Williams, Georg Wohlfahrt, Susumu Yamamoto, Guirui Yu, Roberto Zampedri, Bin Zhao, Xinquan Zhao

Publications and Research

No abstract provided.

Feral Swine Behavior Relative To Aerial Gunning In Southern Texas, Tyler A. Campbell, David Long, Bruce Leland

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) impact resources through their destructive feeding behavior, competition with native wildlife, and impacts to domestic animal agriculture. We studied aerial gunning on feral swine to determine if aerial gunning altered home range and core area sizes, distances between home range centroids, and distances moved by surviving individuals. We collected data before, during, and after aerial gunning in southern Texas. Using Global Positioning System collars deployed on 25 adult feral swine at 2 study sites, we found home range and core area sizes did not differ before and after aerial gunning. However, feral swine moved …

The Economic Impacts To Commercial Farms From Invasive Monkeys In Puerto Rico, Richard M. Engeman, Jose´ E. Laborde, Bernice U. Constantin, Stephanie A. Shwiff, Parker Hall, Anthony Duffiney, Freddie Luciano

United States Department of Agriculture Wildlife Services: Staff Publications

Beginning in the 1930s and continuing through the 1970s, rhesus macaques and patas monkeys were introduced to presumed secure locations, primarily coastal islets, in Puerto Rico. Escapes into the wild began almost immediately after introduction. Today the combined range of the two species covers approximately 600 km² of southwestern Puerto Rico, where serious conflicts with agricultural interests have resulted. The Puerto Rico Department of Agriculture surveyed about 90% of commercial farmers in the range of the monkeys to begin quantifying damage by monkeys and the associated economic losses during the years 2002–2006. During that time, total economic losses by …

An Evaluation Of Diazacon As A Potential Contraceptive In Non-Native Rose-Ringed Parakeets, Mark Lambert, Giovanna Massel, Christi Yoder, David Cowan

United States Department of Agriculture Wildlife Services: Staff Publications

Rose-ringed parakeets (Psittacula krameri) have become widely established outside their native range through accidental or deliberate release. Potential economic impacts on agriculture, conservation concerns, and mixed public opinion regarding the species have highlighted the need to develop effective but humane management options. Fertility control might provide such a solution if a safe and environmentally benign contraceptive was available. The chemical 20,25-diazacholesterol dihydrochloride (diazacon) has previously been used to reduce reproductive output in avian species through reduction of blood cholesterol and cholesterol-dependent reproductive hormones. We orally dosed captive rose-ringed parakeets with a solution of either 9 mg/kg or 18 …

Evaluation Of Feral Swine- Specific Feeder Systems, David B. Long, Tyler A. Campbell, Giovanna Massei

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) have been introduced across many portions of the globe, including rangeland ecosystems of the United States. Feral swine populations are expanding because of their adaptability, high reproductive potential, and because they are (accidentally and intentionally) released by humans. Today, feral swine are the most abundant exotic ungulate in the United States.

Rangeland ecosystems are impacted by feral swine primarily through soil disturbance caused by rooting activities. Within these systems, natural disturbances (e.g., burrowing, grazing by native animals, and periodic fire) generally increase or maintain species diversity. However, rooting by feral swine often occurs at …